CN211697629U

CN211697629U - Multifunctional electrochemical biosensor and analyte testing system

Info

Publication number: CN211697629U
Application number: CN202020132611.2U
Authority: CN
Inventors: 李梦阁
Original assignee: Bioland Technology Shenzhen Co ltd
Current assignee: Bioland Technology Shenzhen Co ltd
Priority date: 2020-01-20
Filing date: 2020-01-20
Publication date: 2020-10-16
Anticipated expiration: 2030-01-20

Abstract

The utility model belongs to the technical field of sample test sensor, a multi-functional electrochemistry biosensor and analyte test system is related to, including the sensor body that connects externally in the analysis appearance, the sensor body is including the substrate layer, electrode layer and the well interlayer that set up range upon range of in proper order, be formed with sampling channel and at least two mutually independent reposition of redundant personnel passageways on the well interlayer, each reposition of redundant personnel passageway is connected in sampling channel's end and is communicated with sampling channel, the electrode layer includes at least two reaction electrodes, a reference electrode and at least two connecting electrodes, reaction electrode and connecting electrode share a reference electrode, each reaction electrode sets up on the reposition of redundant personnel passageway that corresponds, each reposition of redundant personnel passageway is provided with the reference electrode; the reaction electrode is positioned at the front end of the reference electrode; each connecting electrode is connected with the corresponding reaction electrode and is connected with the corresponding external port of the analyzer, and the multifunctional electrochemical biosensor and the analyte testing system have multiple functions, less electrodes and greater clinical significance.

Description

Multifunctional electrochemical biosensor and analyte testing system

Technical Field

The utility model relates to a sample test sensor technical field especially relates to a multi-functional electrochemistry biosensor and analyte test system.

Background

An electrochemical biosensor is an analytical test device in which an immobilized biological component or a biological body itself undergoes a biochemical reaction with electron transfer on an electrode surface to convert the electron transfer generated by the biochemical reaction into an electrical signal that can be used for detection. Electrochemical biosensors are the first biosensors to come out, and have been widely used in the fields of medical care, food industry, agriculture, environmental detection and the like because of their high sensitivity, easy miniaturization, capability of detection in complex system samples and the like.

As an example of blood tests in the field of health care, it is known that clinical blood tests are generally classified into various test categories, mainly including items of whether or not each component in blood is acceptable, four items of blood coagulation, blood sugar, blood fat, blood cholesterol, and various anemias. Taking a blood sugar examination project as an example, currently, an electrochemical blood sugar test paper or a general sensor is usually adopted in each hospital to examine blood sugar, and considering that the existing medical resources are in shortage, the flow of a patient is relatively large all the year round no matter a large hospital or a small hospital, and blood examination is a conventional means for a doctor to diagnose whether the patient has certain diseases or not, so that the situation that the blood test result can be obtained for several hours or even the next day after the patient is queued in the hospital to wait for blood drawing is often seen. One of the reasons why the blood test result is slow in the discovery of the utility model is the product of clinical blood test, such as the test item of the electrochemical blood glucose test paper is single, thereby being not beneficial to improving the blood test efficiency.

SUMMERY OF THE UTILITY MODEL

The utility model discloses aim at solves the inspection item of current clinical blood detection product more single, leads to the lower technical problem of blood detection efficiency.

In order to solve the above technical problem, an embodiment of the present invention provides a multifunctional electrochemical biosensor, which adopts the following technical solutions:

the multifunctional electrochemical biosensor comprises an analyzer with at least two external ports and a sensor body externally connected with the analyzer, wherein the sensor body comprises a base material layer, an electrode layer and a middle interlayer which are sequentially stacked;

a sample feeding channel and at least two mutually independent shunt channels are formed on the middle partition layer, each shunt channel is connected to the tail end of the sample feeding channel and communicated with the sample feeding channel, and a reaction area for biochemical reaction of an analyte is arranged in each shunt channel;

the electrode layer comprises at least two reaction electrodes, a reference electrode and at least two connecting electrodes, and all the reaction electrodes and the connecting electrodes are matched with one reference electrode;

each reaction electrode is arranged on the corresponding shunt channel, and each shunt channel is provided with the reference electrode; on each of the shunt channels, the reaction electrode is positioned at the front end of the reference electrode; and each connecting electrode is connected with the corresponding reaction electrode and is connected with the corresponding external port of the analyzer.

In some embodiments, each of the reaction electrodes is disposed along a width direction of the corresponding shunt channel, and a width of each of the reaction electrodes is greater than a width of the corresponding shunt channel.

In some embodiments, the reference electrode includes at least two first reference sub-electrodes integrally designed, each of the reference sub-electrodes is disposed along a width direction of the corresponding shunt channel and located at a rear end of the corresponding reaction electrode, and a width of each of the reference sub-electrodes is greater than a width of the corresponding shunt channel.

In some embodiments, the length of each reference sub-electrode is greater than the length of the bottom of the corresponding shunt channel.

In some embodiments, the reference electrode further includes at least two second reference sub-electrodes, each of the second reference sub-electrodes is integrally designed with the first reference sub-electrode, and each of the second reference sub-electrodes corresponds to each of the connection electrodes one to one.

In some embodiments, each of the connection electrodes is staggered.

In some embodiments, the multifunctional electrochemical biosensor further comprises an upper cover layer, the upper cover layer being stacked with the middle barrier layer and opposite to the electrode layer.

In some embodiments, the size and shape of each of the diversion channels are the same.

In some embodiments, the shunt channels, the reaction electrodes and the connection electrodes correspond to one another, the number of the shunt channels, the number of the reaction electrodes and the number of the connection electrodes are all 3, and the number of the reference electrodes is 1.

In order to solve the above technical problem, the embodiment of the present invention further provides an analyte testing system, which adopts the following technical solution: the analyte test system includes the multifunctional electrochemical biosensor described above.

Compared with the prior art, the embodiment of the utility model provides a multi-functional electrochemistry biosensor and analyte test system mainly has following beneficial effect:

the multifunctional electrochemical biosensor is characterized in that the middle partition layer is designed to be branched into at least two mutually independent shunt channels from the sample feeding channel, corresponding reaction electrodes are arranged in each shunt channel, at least two connecting electrodes are arranged, the connecting electrodes correspond to the reaction electrodes one to one, and all the reaction electrodes and the connecting electrodes share one reference electrode, so that different biochemical reactions can be generated by shunting reactants into different shunt channels through one sample adding, the biochemical indexes of at least two projects can be displayed in an analyzer at one time, the integral structure is simplified through the sharing of the multifunctional circuit, and in a word, the multifunctional electrochemical biosensor and the analyte testing system are multifunctional, few in electrodes, beneficial to expanding the clinical application range and great in clinical significance.

Drawings

In order to illustrate the solution of the present invention more clearly, the drawings needed for describing the embodiments will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts. Wherein:

FIG. 1 is a schematic diagram of the overall design of a multifunctional electrochemical biosensor including a circuit structure according to an embodiment of the present invention, wherein the top cover layer and the analyzer have been removed;

FIG. 2 is an exploded view of the multifunctional electrochemical biosensor of FIG. 1, wherein the multifunctional electrochemical biosensor has a top cover layer;

FIG. 3 is an enlarged view of a portion of the multifunctional electrochemical biosensor in FIG. 1, wherein the view is mainly used to embody the structure of the reaction region of the sensor;

FIG. 4 is an enlarged view of a portion of the multifunctional electrochemical biosensor of FIG. 1 at B, wherein the view is mainly used to show the layout of the electrodes connecting the sensor body with the analyzer.

The reference numbers in the drawings are as follows:

100. a multifunctional electrochemical biosensor; 10. a sensor body;

1 a substrate layer;

2. an electrode layer; 21. a reaction electrode; 22. a reference electrode; 221. a first reference sub-electrode; 222. a second reference sub-electrode; 23; connecting the electrodes;

3. a middle barrier layer; 31. a sample introduction channel; 32. a flow dividing channel; 33. a reaction zone;

4. an upper cover layer; 41. and (4) a groove.

Detailed Description

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs; the terminology used herein in the description is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention, for example, the terms "length," "width," "upper," "lower," "left," "right," "front," "rear," "vertical," "horizontal," "top," "bottom," "inner," "outer," etc. refer to an orientation or position illustrated in the drawings, which are for convenience of description only and are not to be construed as limiting of the present disclosure.

The terms "including" and "having," and any variations thereof, in the description and claims of this invention and the description of the above figures are intended to cover non-exclusive inclusions; the terms "first," "second," and the like in the description and in the claims, or in the drawings, are used for distinguishing between different objects and not necessarily for describing a particular sequential or chronological order. In the description and claims of the present invention and in the description of the above figures, when an element is referred to as being "fixed" or "mounted" or "disposed" or "connected" to another element, it can be directly or indirectly located on the other element. For example, when an element is referred to as being "connected to" another element, it can be directly or indirectly connected to the other element.

Furthermore, reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the invention. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. It is explicitly and implicitly understood by one skilled in the art that the embodiments described herein can be combined with other embodiments.

It should be noted that the multifunctional electrochemical biosensor 100 belongs to a dry electrochemical biosensor technology, and is mainly used for rapidly detecting biochemical indicators of a blood sample in vitro, and actually, can also be used for detecting other suitable analyte samples, such as liquid samples such as urine. In the case of detecting a blood sample, the multifunctional electrochemical biosensor 100 can be used in medical institutions, home doctors, patients, and the like.

The embodiment of the present invention provides a multifunctional electrochemical biosensor 100, as shown in fig. 1, the multifunctional electrochemical biosensor 100 includes an analyzer (not shown) and a sensor body 10, wherein the analyzer has at least two external ports (not shown), and the sensor body 10 is externally connected to the analyzer. Note that the analyzer is designed according to the function of the sensor body 10. Specifically, each external port in the analyzer is electrically connected to each connecting electrode 23 and reference electrode 22 (specifically, the second reference sub-electrode 222) described below in the sensor body 10, so that the electrochemical signal detected by the sensor body 10 can be displayed on the display of the analyzer by the conversion method of the analyzer, thereby detecting a plurality of reactant items.

In the embodiment of the present invention, as shown in fig. 1 and 2, structurally, the sensor body 10 includes a substrate layer 1, an electrode layer 2, and a middle spacer layer 3 stacked in sequence. In addition, in practice, in the embodiment of the present invention, the multifunctional electrochemical biosensor 100 further includes an upper cover layer 4, wherein the upper cover layer 4 is stacked with the middle partition layer 3 and is opposite to the electrode layer 2, i.e. the upper cover layer 4 is disposed on the top surface of the middle partition layer 3. It is understood that the substrate layer 1, the electrode layer 2, the middle layer 3 and the upper cover layer 4 are sequentially stacked. In this embodiment, the substrate layer 1 may serve as a support substrate, the electrode layer 2 may serve as a design layer of a circuit, the middle spacer layer 3 may serve as a design layer of a channel, and a complete channel structure for reactant sample introduction and reaction may be finally formed by a stacking and matching design between the upper cover layer 4 and the middle spacer layer 3.

Specifically, in this embodiment, the substrate layer 1 may be made of a PET material, but may also be made of other suitable materials. The circuit layer is disposed on the base material layer 1 by screen printing, but may be disposed on the base material layer 1 in other suitable manners. In addition, particularly in the present embodiment, the sensor body 10 of the multifunctional electrochemical biosensor 100 may have a sheet shape for simplifying the structure, that is, the shape of the sensor body may be similar to that of a conventional electrochemical blood glucose test strip.

In the embodiment of the utility model, as shown in fig. 1 to fig. 3, in structural design, be formed with injection channel 31 and two at least reposition of redundant personnel passageways 32 on the intermediate lamella 3, wherein, each reposition of redundant personnel passageway 32 is connected in injection channel 31's end, and communicate with each other with injection channel 31, do not take place to associate for making the testing result, each reposition of redundant personnel passageway 32 is mutually independent, in addition, each reposition of redundant personnel passageway 32 interior has the reaction zone 33 who supplies analyte biochemical reaction, thus, the reactant that gets into from injection channel 31's entry can shunt to in the reposition of redundant personnel passageway 32, biochemical reaction takes place respectively independently, with this can be through a sample application, conveniently once only once detect multiple biochemical index to same reactant fast, also, carry out the detection of a plurality of projects.

It should be noted that, in order to realize biochemical reaction, the reaction area 33 of each branch channel 32 is coated with biochemical active substances, and usually, in order to detect multiple biochemical indexes for one time of the same reactant, the components of the biochemical active substances in each branch channel 32 are different, so that the characteristic components in the reactant such as blood can perform biochemical reaction with each biochemical active substance, thereby obtaining different biochemical results. Of course, they may be the same. Generally, these biochemically active substances may be proteins, enzymes, or the like.

In the embodiment of the present invention, as shown in fig. 1, fig. 2 and fig. 4, in terms of circuit design, the electrode layer 2 includes at least two reaction electrodes 21, one reference electrode 22 and at least two connection electrodes 23, wherein all the reaction electrodes 21 and all the connection electrodes 23 are paired together with one reference electrode 22. That is, all the reaction electrodes 21 share one reference electrode 22, all the connection electrodes 23 share one reference electrode 22, and further all the reaction electrodes 21 and all the connection electrodes 23 share one reference electrode 22, in other words, only 1 reference electrode 22 is provided on the sensor body 10, and one reference electrode 22 is shared for detection of different parameters of the same reactant. Thus, in the conventional design, each time a parameter is detected, at least 4 electrodes are generally required, such as satisfying the detection function, the start function, the full blood detection function, etc., so that 12 electrodes are required for detecting 3 parameters.

In the embodiment of the present invention, as shown in fig. 1 and 3, in order to realize biochemical reaction, each reaction electrode 21 is disposed on the corresponding flow dividing channel 32, and each flow dividing channel 32 is provided with the reference electrode 22. In each of the flow-dividing channels 32, the reaction electrode 21 is located at the front end of the reference electrode 22, that is, after the reactant is divided into the corresponding flow-dividing channel 32 from the sample inlet channel 31, the reactant needs to pass through the corresponding reaction electrode 21 first and then pass through the reference electrode 22. Correspondingly, in order to facilitate the transmission of the results of the biochemical reaction to the analyzer, each connecting electrode 23 is connected to the corresponding reaction electrode 21 and is connected to a corresponding external port of the analyzer (not shown).

It can be understood that the shunt channels 32, the reaction electrodes 21 and the connection electrodes 23 correspond to each other one by one, and each of the reaction electrodes 21 and the reference electrode 22 together detect one parameter, so that the number of the shunt channels 32, the reaction electrodes 21 and the connection electrodes 23 is equal to the number of parameters or items that the multifunctional electrochemical biosensor 100 can detect.

Specifically, in the present embodiment, the number of the flow-dividing channels 32, the reaction electrodes 21, and the connection electrodes 23 is 3, and the number of the reference electrodes 22 is 1. Taking the reactant as blood as an example, in the embodiment, the multifunctional electrochemical biosensor 100 can be used to detect three indexes of blood sugar, blood fat and blood cholesterol. In fact, of course, other different functional indexes can be designed according to different requirements, such as four blood fat items, liver function, kidney function and the like can be detected simultaneously.

For convenience of introduction, taking the detection of three parameters as an example, as shown in fig. 1 and fig. 3, the sample injection channel 31 and each of the flow splitting channels 32 are respectively numbered as 1, 2, 3 and 4, wherein the channel 1 is the sample injection channel 31, so that the reactant can be split into the

channels

2, 3 and 4 after being injected from the channel 1. Corresponding to each shunt channel 32, each reaction electrode 21 is an a, b and c electrode, and the a, b and c electrodes correspond to a reference electrode d (also referred to as a first reference sub-electrode 221), and in addition, each connection electrode 23 connected with the analyzer is an a1, b1 and c1 electrode, so that in the No. 2 shunt channel 32, the reactant passes through the reaction electrode a and the reference electrode d in sequence, and then the corresponding reaction signal is transmitted to the analyzer through the corresponding external port by the connection electrode a1, so that one parameter can be detected; in the No. 3 shunt channel 32, the reactant passes through the reaction electrode b and the reference electrode d in sequence, and then the corresponding reaction signal is transmitted to the analyzer through the corresponding external port by the connecting electrode b1, so that another parameter can be detected; in the flow-dividing channel No. 4, the reactant passes through the reaction electrode c and the reference electrode d in sequence, and then the corresponding reaction signal is transmitted to the analyzer through the corresponding external port by the connecting electrode c1, so that another parameter can be detected.

In summary, compared with the prior art, the multifunctional electrochemical biosensor 100 has at least the following beneficial effects: the multifunctional electrochemical biosensor 100 is designed in such a way that the middle partition layer 3 is branched into at least two mutually independent shunt channels 32 from the sample feeding channel 31, each shunt channel 32 is provided with a corresponding reaction electrode 21, at least two connecting electrodes 23 are arranged, each connecting electrode 23 corresponds to the corresponding reaction electrode 21 one by one, and all the reaction electrodes 21 and the connecting electrodes 23 share one reference electrode 22, so that different biochemical reactions can be generated by shunting reactants into different shunt channels 32 through one sample feeding, the biochemical indexes of at least two items can be displayed in an analyzer at one time, and the overall structure is simplified through the sharing of a multifunctional circuit.

In order to make the technical solution of the present invention better understood, the technical solution of the embodiment of the present invention will be clearly and completely described below with reference to fig. 1 to 4.

In some embodiments, as shown in fig. 1 and 3, each reaction electrode 21 is disposed along the width direction of the corresponding flow dividing channel 32, and the width of each reaction electrode 21 is greater than the width of the corresponding flow dividing channel 32. Thus, it is ensured that the printing error does not affect the area of each reaction electrode 21.

In some embodiments, as shown in fig. 1 and 3, the reference electrode 22 includes at least two first reference sub-electrodes 221 integrally designed, wherein each reference sub-electrode is disposed along the width direction of the corresponding shunt channel 32 and located at the rear end of the corresponding reaction electrode 21, and the width of each reference sub-electrode is greater than the width of the corresponding shunt channel 32, so that the influence of printing errors can be eliminated.

In some embodiments, as shown in fig. 1 and 3, each reference sub-electrode has a length that is greater than the length of the bottom of the corresponding shunt channel 32. Thus, the reference electrode 22 can detect whether the sample injection is sufficient or not, and the full blood detection can be performed.

In some embodiments, as shown in fig. 1 and 4, in order to facilitate the transmission of different biochemical reaction results to the analyzer, the reference electrode 22 further comprises at least two second reference sub-electrodes 222, wherein each second reference sub-electrode 222 is integrally designed with the first reference sub-electrode 221, and each second reference sub-electrode 222 is in one-to-one correspondence with each connecting electrode 23. It is understood that each second reference sub-electrode 222 and each first reference sub-electrode 221 actually belong to the same reference electrode 22. In addition, in the present embodiment, any two second reference sub-electrodes 222 can be used as the starting electrodes for starting the analyzer, so that the number of electrodes can be further reduced.

Specifically, in the present embodiment, the three second reference sub-electrodes 222 are d1, d2 and d3, respectively, but in reality, they are the same reference electrode 22 as the reference electrode d.

In some embodiments, as shown in fig. 1 and 4, the connecting electrodes 23, such as the a1, b1 and c1 electrodes, are staggered, so that the electrodes with different functions can be prevented from being mistakenly contacted or short-circuited.

In some embodiments, as shown in fig. 1 and 3, the diversion channels 32 are the same size and shape. Like this, can conveniently carry out the same amount and divide the appearance to detect, in fact of course, each reposition of redundant personnel passageway 32 size can be different, correspondingly, each reposition of redundant personnel passageway 32 shape also can be different, and specifically can be according to actual need and decide. It should be noted that, a groove 41 is formed on the side of the upper cover layer 4 close to the middle partition layer 3, so as to be conveniently matched with the sample feeding channel 31 and the shunt channel 32 formed on the middle partition layer 3 to form a complete channel together.

It should be noted that, in fact, the multifunctional electrochemical biosensor 100 may further include a biological recognition element, a signal converter, etc. to ensure that the sensor can realize complete detection, but the structure of these components can adopt the existing structure, and the protection key points of the present invention are not described herein, so that the details are not repeated herein.

As can be understood from the above, in the present embodiment, the multifunctional electrochemical biosensor 100 has the following features: 1) the kit is multifunctional, and can detect various biochemical indexes at one time; 2) the whole body only adopts one reference electrode 22, and the number of the used electrodes is reduced through electrode sharing, so that the whole structure is simplified, the manufacturing cost is reduced, and the reference electrode 22 can be used as the shared reference electrode 22, a starting electrode for starting the analyzer and a full blood detection electrode for detecting the sample injection amount of a sample.

Based on above-mentioned multi-functional electrochemical biosensor 100, the embodiment of the present invention further provides an analyte test system, wherein, this analyte test system includes above-mentioned multi-functional electrochemical biosensor 100, it should be noted that, this analyte test system can be used for the detection to each function index of blood, still can detect each index of other liquid flows such as urine.

In summary, compared with the prior art, the analyte test system has at least the following beneficial effects: by adopting the multifunctional electrochemical biosensor 100, the analyte testing system has various functions, simple structure and low manufacturing cost, is beneficial to expanding the clinical application range and has great clinical significance.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.

Claims

1. The multifunctional electrochemical biosensor is characterized by comprising an analyzer with at least two external ports and a sensor body externally connected with the analyzer, wherein the sensor body comprises a substrate layer, an electrode layer and a middle interlayer which are sequentially stacked;

2. The multifunctional electrochemical biosensor as claimed in claim 1, wherein each of the reaction electrodes is disposed along a width direction of the corresponding flow dividing channel, and the width of each of the reaction electrodes is greater than the width of the corresponding flow dividing channel.

3. The multifunctional electrochemical biosensor of claim 1, wherein the reference electrode comprises at least two first reference sub-electrodes integrally designed, each of the reference sub-electrodes is disposed along the width direction of the corresponding shunt channel and located at the rear end of the corresponding reaction electrode, and the width of each of the reference sub-electrodes is greater than the width of the corresponding shunt channel.

4. The multifunctional electrochemical biosensor of claim 3, wherein each of said reference sub-electrodes has a length greater than the length of the bottom of the corresponding flow-splitting channel.

5. The multifunctional electrochemical biosensor of claim 3, wherein said reference electrode further comprises at least two second reference sub-electrodes, each of said second reference sub-electrodes is integrally designed with said first reference sub-electrode, and each of said second reference sub-electrodes corresponds to each of said connection electrodes.

6. The multifunctional electrochemical biosensor of claim 1, wherein each of said connecting electrodes is staggered.

7. The multifunctional electrochemical biosensor of claim 1 further comprising a top cover layer disposed in a stack with the separator layer opposite the electrode layer.

8. The multifunctional electrochemical biosensor as claimed in any one of claims 1 to 7, wherein each of the diversion channels is identical in size and shape.

9. The multifunctional electrochemical biosensor of any one of claims 1 to 7, wherein the shunt channels, the reaction electrodes and the connection electrodes correspond to each other one by one, and the number of the shunt channels, the number of the reaction electrodes and the number of the connection electrodes are 3, and the number of the reference electrodes is 1.

10. An analyte test system, characterized in that it comprises a multifunctional electrochemical biosensor according to any one of claims 1 to 9.