CN211697194U

CN211697194U - Low-temperature filtering and uniformly mixing device for protein purification

Info

Publication number: CN211697194U
Application number: CN202020217221.5U
Authority: CN
Inventors: 牛晓旭; 张文婧; 赵巧辉; 李桂林; 付光宇; 吴学炜; 杨增利
Original assignee: Zhengzhou Immuno Biotech Co Ltd
Current assignee: Zhengzhou Immuno Biotech Co Ltd
Priority date: 2020-02-27
Filing date: 2020-02-27
Publication date: 2020-10-16
Anticipated expiration: 2030-02-27

Abstract

The utility model discloses a low-temperature filtering and mixing device for protein purification, which comprises a constant-temperature magnetic stirrer and an upper opening stirring pot which is arranged on the constant-temperature magnetic stirrer in a matching way, wherein a stirring rotor which is controlled to rotate by the constant-temperature magnetic stirrer is arranged at the center of the inner bottom surface of the stirring pot; an upper opening insulation can covering the outer side of the stirring pot is arranged on the top surface of the constant-temperature magnetic stirrer, and an insulation partition plate is horizontally arranged at the position, close to the top, of the inner cavity of the insulation can; an insertion hole is formed in the heat-insulating partition plate, a filtrate funnel with a conical structure is inserted into the insertion hole in a matched manner and is positioned in a cavity above the heat-insulating partition plate, and a liquid outlet at the bottom of the filtrate funnel is communicated with a flow guide pipe which vertically extends into the stirring pot downwards. The utility model has the advantages of simple structure, simple operation, safety and high efficiency; when satisfying conventional protein purification operation, realize filtering and the mixing to the protein under the low temperature environment, the effectual experimenter of having avoided works under the long-term low temperature environment of department, improves work efficiency, reduces the experiment cost.

Description

Low-temperature filtering and uniformly mixing device for protein purification

Technical Field

The utility model belongs to the technical field of the protein purification technique and specifically relates to a low temperature filtration, mixing device are used in protein purification.

Background

At present, in the biological medicine industry, protein purification relates to pretreatment and process treatment of a protein sample solution, and low-temperature filtration and uniform mixing operation of the protein sample solution at a lower temperature are required. In order to ensure that the purified protein sample has better activity and stability, the laboratory personnel are often required to carry out filtering and mixing operation in a refrigerating chamber, and adverse effects on the health of the working personnel can be caused for a long time; and the staff who operates under the cold-stored environment can lead to operating time extension because of the reduction of manual flexibility, and work efficiency reduces, and unexpected risk factor increases, and the accuse temperature cost of walk-in chamber is relatively great in addition, can increase the resource consumption, causes a large amount of wasting of resources.

Disclosure of Invention

The utility model aims to provide a simple structure, it is easy and simple to handle, safe efficient low temperature filtration, mixing device for protein purification.

In order to achieve the above purpose, the utility model can adopt the following technical proposal:

the utility model discloses a low-temperature filtering and mixing device for protein purification, which comprises a constant-temperature magnetic stirrer and an upper opening stirring pot which is arranged on the constant-temperature magnetic stirrer in a matching way, wherein a stirring rotor which is controlled to rotate by the constant-temperature magnetic stirrer is arranged at the center of the inner bottom surface of the stirring pot; an upper opening heat preservation box which is covered on the outer side of the stirring pot is arranged on the top surface of the constant-temperature magnetic stirrer, and a heat preservation partition plate is horizontally arranged at the position, close to the top, of the inner cavity of the heat preservation box; the stirring kettle is characterized in that an insertion hole is formed in the heat-insulating partition plate, a filtrate funnel with a conical structure is inserted into the insertion hole in a matching manner and located in a cavity above the heat-insulating partition plate, and a drainage pipe vertically extending downwards into the stirring kettle is communicated with a liquid outlet at the bottom of the filtrate funnel.

The front side wall of the constant-temperature magnetic stirrer is provided with a rotating speed control panel, a temperature control panel and a power switch, and the side wall of the constant-temperature magnetic stirrer extends outwards to be provided with an external power line.

An upper cover for covering a cavity above the heat insulation partition plate is hinged on the opening at the top of the heat insulation box, a front opening for taking and placing the stirring pot is formed in the front side wall of the heat insulation box, and a front opening door is hinged on the front opening; the upper cover, the front opening door and the heat-insulating partition plate are all transparent plates; handles which are convenient to hold are arranged on the outer side surfaces of the upper cover and the front opening door; and silica gel sealing strips used for plugging gaps are arranged on the outer edges of the upper cover and the front opening door.

The utility model has the advantages of simple structure, easy operation, safety and high efficiency. The filtrate funnel and the stirring pot cover of the constant-temperature magnetic stirrer are arranged in the heat insulation box to work, so that the filtration and the uniform mixing of the protein in a low-temperature environment are realized while the conventional protein purification operation is met, the filtration and the uniform mixing efficiency is ensured, the experimental controllability is enhanced, the experiment personnel are effectively prevented from working in the low-temperature environment for a long time, the personal health of the experiment personnel is ensured, the working efficiency during the operation is improved, and the experimental cost is reduced; in addition, the whole filtering and mixing process does not need to use a refrigerating chamber, so that the temperature control cost is greatly reduced, the resource consumption is reduced, and the resource waste is avoided.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Detailed Description

As shown in figure 1, protein purification is with low temperature filtration, mixing device, including constant temperature magnetic stirrers 1 and supporting upper shed agitator kettle 2 that sets up on it, agitator kettle 2 is used for accepting the albumen sample solution of treating the mixing, the interior bottom surface center department of agitator kettle 2 is provided with by constant temperature magnetic stirrers 1 control pivoted stirring rotor 3. Specifically, a rotating speed control panel 9, a temperature control panel 10 and a power switch 11 are arranged on the front side wall of the constant-temperature magnetic stirrer 1, an external power line 12 is arranged on the side wall of the constant-temperature magnetic stirrer 1 in an outward extending mode, the rotating speed control panel 9 and the temperature control panel 10 are touch panels with liquid crystal display, and the constant-temperature magnetic stirrer 1 is used for controlling the stirring rotor 3 to rotate so as to uniformly mix protein sample solution contained in the stirring pot 2; in addition, in the process of uniformly mixing, the constant-temperature magnetic stirrer 1 can continuously cool the protein sample solution in the stirring pot 2, so that the protein sample solution is stirred in a low-temperature environment all the time, and the activity of the protein sample solution is ensured.

An upper opening insulation can 4 which covers the outer side of the stirring pot 2 is arranged on the top surface of the constant-temperature magnetic stirrer 1, an insulation partition plate 5 is horizontally arranged at the position, close to the top, of an inner cavity of the insulation can 4, and the loss of temperature in the process of uniformly mixing the protein sample solution is slowed down through the insulation can 4; an insertion hole 6 is formed in the heat-insulating partition plate 5, a filtrate funnel 7 with a conical structure is inserted into the insertion hole 6 in a matching manner and located in a cavity above the heat-insulating partition plate 5, a liquid outlet at the bottom of the filtrate funnel 7 is communicated with a flow guide pipe 8 which extends into the stirring pot 2 vertically and downwards, and the flow guide pipe 8 and the filtrate funnel 7 are of an integral structure.

In order to prevent the filtrate funnel 7 from being exposed outside for a long time, an upper cover 13 covering a cavity above the heat-insulating partition plate 5 is hinged on the top opening of the heat-insulating box 4; in addition, a front opening which is convenient for taking and placing the stirring pot 2 is arranged on the front side wall of the heat preservation box 4, and a front opening door 14 is hinged on the front opening; the upper cover 13, the front opening door 14 and the heat-insulating partition plate 5 are transparent plates, so that observation is facilitated; handles 15 which are convenient to hold are arranged on the outer side surfaces of the upper cover 13 and the front opening door 14; in addition, in order to improve the sealing performance of the upper cover 13 and the front opening door 14, a silicone sealing strip 16 for sealing the gap is arranged on the outer edges of the upper cover 13 and the front opening door 14.

When in use, firstly, the stirring pot 2 is placed in the heat preservation box 4 through the front opening of the heat preservation box, then the filtrate funnel 7 is inserted into the insertion hole 6 on the heat preservation partition plate 5 from top to bottom, the guide pipe 8 at the lower part of the filtrate funnel 7 just sticks to the inner side wall of the stirring pot 2 and extends into the stirring pot, and the lower end of the guide pipe 8 is close to the inner bottom surface of the stirring pot 2; then, proper filter paper is selected to be placed in the filtrate funnel 7, the upper cover 13 is buckled, the filter paper is connected with an external power line 12 of the constant-temperature magnetic stirrer 1, a power switch 11 is turned on, the temperature control panel 10 is adjusted according to the experiment requirements, and the filter paper is turned on to be precooled for 30-90 min; after the temperature in the heat preservation box 4 reaches the set temperature, the upper cover 13 is opened, the protein sample solution to be filtered is poured into the filtrate funnel 7, the rotating speed control panel 9 is adjusted and opened, the protein sample solution filtered by the filtrate funnel 7 is uniformly mixed and stirred until all the protein sample solution is filtered, the filtrate funnel 7 is taken out, the upper cover 13 is closed, the stirring is continued for 20-40 min at a low temperature, the uniform mixing of the protein sample is ensured, the constant-temperature magnetic stirrer 1 can be closed, and the front opening door 14 is opened to take out the stirring pot 2 for next experimental operation.

Claims

1. A low-temperature filtering and uniformly mixing device for protein purification comprises a constant-temperature magnetic stirrer (1) and an upper opening stirring pot (2) which is arranged on the constant-temperature magnetic stirrer in a matched manner, wherein a stirring rotor (3) which is controlled to rotate by the constant-temperature magnetic stirrer (1) is arranged at the center of the inner bottom surface of the stirring pot (2); the method is characterized in that: an upper opening insulation can (4) which is covered on the outer side of the stirring pot (2) is arranged on the top surface of the constant-temperature magnetic stirrer (1), and an insulation partition plate (5) is horizontally arranged at the position, close to the top, of the inner cavity of the insulation can (4); the improved stirring kettle is characterized in that an insertion hole (6) is formed in the heat-insulating partition plate (5), a filtrate funnel (7) with a conical structure is arranged in a cavity above the heat-insulating partition plate (5) in the insertion hole (6) in an adaptive insertion mode, and a drainage pipe (8) which vertically extends into the stirring kettle (2) downwards is communicated with a liquid outlet at the bottom of the filtrate funnel (7).

2. The low-temperature filtering and uniformly mixing device for protein purification according to claim 1, which is characterized in that: the front side wall of the constant-temperature magnetic stirrer (1) is provided with a rotating speed control panel (9), a temperature control panel (10) and a power switch (11), and the side wall of the constant-temperature magnetic stirrer (1) extends outwards to be provided with an external power line (12).

3. The low-temperature filtering and uniformly mixing device for protein purification according to claim 1, which is characterized in that: an upper cover (13) covering a cavity above the heat preservation partition plate (5) is hinged to the top opening of the heat preservation box (4), a front opening convenient for taking and placing the stirring pot (2) is formed in the front side wall of the heat preservation box (4), and a front opening door (14) is hinged to the front opening.

4. The low-temperature filtering and uniformly mixing device for protein purification according to claim 3, which is characterized in that: the upper cover (13), the front opening door (14) and the heat-insulating partition plate (5) are all transparent plates.

5. The low-temperature filtering and uniformly mixing device for protein purification according to claim 3, which is characterized in that: handles (15) convenient to hold are arranged on the outer side surfaces of the upper cover (13) and the front opening door (14).

6. The low-temperature filtering and uniformly mixing device for protein purification according to claim 3, which is characterized in that: and silica gel sealing strips (16) for plugging gaps are arranged on the outer edges of the upper cover (13) and the front opening door (14).