CN211696982U - Nematode sampling separator - Google Patents

Abstract

The utility model discloses a nematode sampling separator, which comprises a separator body, wherein the separator body comprises an extraction bottle, a separation bottle and a storage bottle; a screen mesh and a reversible retaining screen are sequentially distributed in the separation bottle from top to bottom; the lower end of the separation bottle is opened and is detachably connected and communicated with the storage bottle; the turnover intercepting sieve is circular when viewed from above, the diameter of the turnover intercepting sieve is matched with the diameter of an inner cavity of the separating bottle, and the turnover intercepting sieve is rotatably connected with the separating bottle through a rotating shaft. Adopt the utility model has the advantages of to pine wood nematode class separation effectual, impurity such as separation back saw-dust, billet remain few, live worm extraction rate height.

Description

Nematode sampling separator

Technical Field

The utility model relates to a nematode sampling separation technical field such as woods, crops, concretely relates to nematode sampling separator.

Background

Nematodes can be parasitic on more than 2000 plants and can be rapidly propagated with a short life history. In recent years, nematodes are seriously generated in successive years, the generation area is always increased, 21 provinces in China have reports, and particularly, the pine wood nematodes and the root-knot nematodes become serious diseases of pines and crops all over the world along with the continuous expansion of the generation situation. And it is very necessary to the insect pest early warning to the nematode sampling, however when needs carry out the sampling separation, it is too many based on the impurity such as saw-dust, the billet, the root of a tree, perhaps the clod often is the cubic viscosity, adopt violent stirring to cause the nematode death easily, it is unfavorable to follow-up data detection, and sample and impurity viscous bottom of sinking after the leaching are difficult to realize the nematode separation, adopt simple sieving, the too close nematode of screen cloth is held back, and the simple settlement of conventional screen cloth also can appear a large amount of impurity and finally also can't effectively separate, and the impurity that contains too much also can't use subsequent automatic checkout device.

The above background disclosure is only provided to aid in understanding the concepts and technical solutions of the present invention, and it does not necessarily belong to the prior art of the present patent application, and it should not be used to assess the novelty and inventive step of the present application without explicit evidence that the above content has been disclosed at the filing date of the present patent application.

SUMMERY OF THE UTILITY MODEL

The utility model provides a to above-mentioned technical problem provide a sampling separation is effectual, the impurity remains few nematode sampling separator and sampling separation method after the separation.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a nematode sampling separator, the separator body comprises an extraction bottle, a separation bottle and a storage bottle; a screen mesh and a reversible retaining screen are sequentially distributed in the separation bottle from top to bottom; the lower end of the separation bottle is opened and is detachably connected and communicated with the storage bottle; the turnover intercepting sieve is circular when viewed from above, the diameter of the turnover intercepting sieve is matched with the diameter of an inner cavity of the separating bottle, and the turnover intercepting sieve is rotatably connected with the separating bottle through a rotating shaft.

Furthermore, the turnover interception sieve is in a shape of a hemispherical groove, the main body is made of a gauze with more than 300 meshes, and the upper part of the main body is combined with a circle of sealing ring.

Furthermore, the tail end of the rotating shaft is radially provided with a spherical plunger, and a shaft sleeve at the tail end of the rotating shaft is internally provided with a clamping hole corresponding to the spherical plunger; the other end of the rotating shaft is provided with a manual thumb wheel; two or more than two turnover retaining screens are distributed in sequence from top to bottom.

Furthermore, a funnel-shaped inner cavity is arranged at the middle upper part of the separation bottle, and the sieve is distributed in the funnel-shaped inner cavity; the screen mesh comprises a coarse screen and a fine screen; the coarse screen is made of a screen with 30 meshes, 40 meshes or 50 meshes; the fine screen is made of a screen mesh of 100-300 meshes; the coarse screen is distributed above the fine screen.

Furthermore, it is equipped with a plurality of fixture blocks to correspond on the inner chamber that leaks hopper-shaped, the coarse screen with the fine screen outer fringe corresponds the draw-in groove has been seted up to the fixture block, and passes through the draw-in groove with the connection can be dismantled to the separation bottle.

Further, the lower part of the separation bottle is in a funnel-shaped structure, and an opening at the lower end of the separation bottle is communicated with the storage bottle.

Further, the leaching bottle comprises a bottle body and a bottle cap; the bottle cover is distributed with a pressure bar; a spring groove is axially formed in the middle of the bottle cap; the compression bar sequentially penetrates through the spring groove and the bottle cap; springs are distributed in the spring grooves; the middle upper part of the pressure lever is provided with a spring limiting block corresponding to the spring, and the spring is distributed below the spring limiting block; the lower end of the pressure rod is provided with a pressing net; the immersion-pressing net is made of a 10-20 mesh screen.

Further, the leaching bottle is detachably connected to the upper part of the separation bottle.

Further, still include the safety cover, the cover can be dismantled to the safety cover and overlap the outside of separation bottle.

The utility model has the advantages of compared with the prior art:

(1) the utility model is provided with the leaching bottle, adopts the mode of the pressure rod, the spring and the leaching net component, and repeatedly moves up and down in the extracting agent through the leaching net, so that the crude extract of the collected sample is quickly and fully scattered, and the nematode is directly extruded without hard objects, thereby preventing the hard objects from causing damage and death of the nematode, and preserving the survival rate of the nematode in the extracting solution to the maximum extent; meanwhile, the problems that in the traditional leaching process, samples and impurities are deposited at the bottom of the bottle and are adhered to the bottom of the bottle, and nematodes penetrate into impurity layers at the bottom of the bottle due to the fact that the nematodes move downwards under the action of gravity in water, and cannot be leached effectively and the like are solved; by adopting the reticular leaching net, impurities such as sawdust, wood blocks, tree roots and the like which are usually remained are effectively coarsely screened under the condition of improving the efficiency of scattering and screening nematodes by crude extracts, and the leaching effect is good.

(2) The utility model discloses a be equipped with and to overturn and hold back the sieve, adopt fine and close screen cloth, ensure to permeate water, realize the effect of holding back of fine impurity in to the leaching solution, intercept it earlier through utilizing nematode downstream and fine impurity settling effect principle promptly, then hold back the sieve to overturning, the face down is held back to overturning this moment, utilizes the characteristic of gravity guide nematode downstream promptly, makes the nematode further downstream, and fine impurity is detained by fine and close screen cloth, uses 2 and above through the coincide can overturn and hold back the sieve and ensure more down fine impurity less, and obtain more pressing close to actual live nematode quantity and kind, draw high-efficient, convenient, effectual.

(3) The utility model discloses a be equipped with coarse screen and fine screen, can further divide the sieve to the leaching solution, do benefit to the dismantlement washing and the change in later stage through establishing into the dismantlement mode of dismantling.

(4) The utility model discloses a be equipped with transparent safety cover, realize the protection to storage bottle and manual thumb wheel.

Drawings

Fig. 1 is a cross-sectional view of the present invention;

fig. 2 is a schematic view of the reversible retention screen of the present invention.

Detailed Description

As shown in figures 1-2, the nematode sampling separator comprises a separator body, a leaching bottle, a separation bottle 7 and a storage bottle 16. The leaching bottle comprises a bottle body 5 and a bottle cap 2; the bottle cap 2 is connected with the bottle body 5 through a thread structure, and a sealing gasket can be arranged at the connection part for improving the compactness; a pressure rod 4 is distributed on the bottle cap 2; a spring groove 2-1 is axially formed in the middle of the bottle cap 2; the compression bar 4 sequentially penetrates through the spring groove 2-1 and the bottle cap 2, and a sealing ring can be arranged at a movable contact position of the compression bar to improve compactness; springs 3 are distributed in the spring grooves 2-1; the middle upper part of the pressure lever 4 is provided with a spring limiting block corresponding to the spring 3, the spring 3 is distributed below the spring limiting block, and the spring 3 extrudes the spring limiting block upwards to play a role in resetting when in use; the lower end of the pressure lever 4 is provided with a pressing net 6; the impregnation and pressing net 6 is made of a 10-20 mesh screen. The lower extreme of leaching bottle of this embodiment can dismantle through helicitic texture and connect in the upper portion of separation bottle 7 links into an organic whole, also plays the bottle lid effect of separation bottle 7 promptly.

A screen mesh and a reversible retention screen 11 are sequentially distributed in the separation bottle 7 from top to bottom; a funnel-shaped inner cavity 10 is formed in the middle upper part of the separation bottle 7, and the screen is distributed in the funnel-shaped inner cavity 10; the screen of this embodiment comprises a coarse screen 9 and a fine screen 8; the coarse screen 9 is made of a screen with 30 meshes, 40 meshes or 50 meshes; the fine screen 8 is made of a screen mesh of 100-300 meshes; the coarse screen 9 is distributed above the fine screen 8 and mainly plays a role in further screening impurities. This embodiment it is equipped with a plurality of fixture blocks to let out funnel shaped inner chamber 10 on the correspondence, coarse screen 9 with 8 outer fringe of fine screen correspond the draw-in groove has been seted up to the fixture block, and passes through the draw-in groove with the connection can be dismantled to separation bottle 7, does benefit to later stage sieve and separation bottle and can overturn and hold back the washing operation of sieve 11. The turnover intercepting sieve 11 is circular when overlooked, the diameter of the turnover intercepting sieve is matched with the diameter of the inner cavity of the separation bottle 7, and two sides of the turnover intercepting sieve are rotatably connected with the separation bottle 7 through a rotating shaft. The reversible retaining screen 11 of this embodiment is shaped like a hemispherical groove, and of course, it may be designed as a slightly concave groove mesh screen structure or a circular plane mesh screen structure, its main body is made of gauze with more than 300 meshes, and its upper portion is combined with a circle of sealing ring 11-1 to improve compactness, and therefore it is made of dense gauze, mainly considering its retaining performance for fine impurities and ensuring the stability of water pressure in the bottle during the turnover process of the reversible retaining screen 11, and ensuring the smooth operation. The tail end of the rotating shaft on one side is radially provided with a spherical plunger 13, a shaft sleeve 14 at the tail end of the rotating shaft is internally provided with a clamping hole corresponding to the spherical plunger, the spherical plunger 13 is mainly used for preventing the turnover intercepting screen 11 from swinging or repeatedly turning back after the turnover intercepting screen 11 is turned down, and certainly, the clamping hole and the spherical plunger 13 are matched in arrangement position, such as horizontal arrangement, so as to ensure that the turnover intercepting screen 11 is fixed in a horizontal direction in a clamping state; of course, the above function can be achieved by pressing the sealing ring 11-1 without providing the spherical plunger 13, but the effect of the continuous use of the sealing ring is inferior to that of the above structure; a manual shifting wheel 12 is arranged at one end of the rotating shaft at the other side, and the turning operation is realized through the manual shifting wheel 12; two or more than two turnover retaining screens 11 are distributed in sequence from top to bottom. The lower part of the separation bottle 7 is of a funnel-shaped structure, and the opening at the lower end of the separation bottle is communicated with the storage bottle 16 and is detachably connected through a thread structure. For preventing the outdoor collision of carrying the process to storing bottle 16 and manual thumb wheel, improve the protecting effect, this embodiment still includes safety cover 15, safety cover 15 adopts transparent material to constitute, can dismantle the cover the outside of separation bottle 7, of course, in order to facilitate the observation, this embodiment leaching bottle, separation bottle 7 and storing bottle 16 all adopt transparent material to make.

The nematode sampling and separating method by adopting the nematode sampling separator comprises the following steps:

s1: loading a sample, namely pouring a certain amount of purified water/extractant into the leaching bottle, then inserting the pressing net into the middle upper part of the leaching bottle, loading a sample into the leaching bottle and placing the sample on the pressing net, and then tightening the bottle cap;

s2: scattering and leaching, and repeatedly and slowly pressing the pressure rod to ensure that the crude sample in the leaching bottle is fully stirred and mixed with the purified water/the extracting agent; until the sample is completely scattered and no obvious sample and impurities such as wood chips, barks, clods and the like are adhered to the bottom of the bottle, the leaching is completed;

s3: coarse filtration, namely taking out the leaching net after the leaching operation is finished, and discarding impurities such as sawdust, barks, wood blocks and the like remained on the leaching net; then pouring a certain amount of purified water/extractant into the separation bottle, pouring the leaching liquor into the separation bottle, rinsing the leaching bottle with purified water/extractant, and pouring rinsing liquid into the separation bottle, and finally ensuring that the liquid amount in the separation bottle is not higher than the coarse screen, so as to ensure that the liquid below the coarse screen in the separation bottle is filled in the whole separation process and ensure the separation effect of each separation link of the nematodes (the separation is based on the characteristic of utilizing the downward movement of the nematodes in water); settling operation (which means that the bottle body is usually kept still, but the phenomenon that the bottle body is slightly bounced to promote the movement of the nematodes which tend to move downwards in water is not excluded) for 10-30 min;

s4: settling and retaining, namely turning the uppermost reversible retaining screen (turning the manual thumb wheel by 180 degrees), performing settling operation for 10-20 min, turning the next reversible retaining screen, and repeating the operation until the lowermost reversible retaining screen is turned, and performing settling operation for 10-20 min;

s5: and finally, taking down the storage bottle to finish the separation operation, and finally, separating the pests in the storage bottle to be used for living observation under a microscope or automatic detection of species/quantity and the like.

Through adopting the technical scheme of the utility model, compare prior art scheme and realize following beneficial effect:

Claims (9)

1. the utility model provides a nematode sampling separator, includes the separator body, its characterized in that: the separator body comprises a leaching bottle, a separation bottle and a storage bottle; a screen mesh and a reversible retaining screen are sequentially distributed in the separation bottle from top to bottom; the lower end of the separation bottle is opened and is detachably connected and communicated with the storage bottle; the turnover intercepting sieve is circular when viewed from above, the diameter of the turnover intercepting sieve is matched with the diameter of an inner cavity of the separating bottle, and the turnover intercepting sieve is rotatably connected with the separating bottle through a rotating shaft.

2. A nematode sampling separator as claimed in claim 1, wherein: the reversible intercepting screen is in a hemispherical groove shape, the main body is made of gauze with more than 300 meshes, and the upper part of the main body is combined with a circle of sealing ring.

3. A nematode sampling separator as claimed in claim 1, wherein: the tail end of the rotating shaft is radially provided with a spherical plunger, and a shaft sleeve at the tail end of the rotating shaft is internally provided with a clamping hole corresponding to the spherical plunger; the other end of the rotating shaft is provided with a manual thumb wheel; two or more than two turnover retaining screens are distributed in sequence from top to bottom.

4. A nematode sampling separator as claimed in claim 1, wherein: a funnel-shaped inner cavity is formed in the middle upper part of the separation bottle, and the screen is distributed in the funnel-shaped inner cavity; the screen mesh comprises a coarse screen and a fine screen; the coarse screen is made of a screen with 30 meshes, 40 meshes or 50 meshes; the fine screen is made of a screen mesh of 100-300 meshes; the coarse screen is distributed above the fine screen.

5. A nematode sampling separator as claimed in claim 4, wherein: the funnel-shaped inner cavity is correspondingly provided with a plurality of clamping blocks, the coarse screen and the fine screen are correspondingly provided with clamping grooves on the clamping blocks, and the clamping grooves are detachably connected with the separation bottle.

6. A nematode sampling separator as claimed in claim 1, wherein: the lower part of the separation bottle is of a funnel-shaped structure, and an opening at the lower end of the separation bottle is communicated with the storage bottle.

7. A nematode sampling separator as claimed in claim 1, wherein: the leaching bottle comprises a bottle body and a bottle cap; the bottle cover is distributed with a pressure bar; a spring groove is axially formed in the middle of the bottle cap; the compression bar sequentially penetrates through the spring groove and the bottle cap; springs are distributed in the spring grooves; the middle upper part of the pressure lever is provided with a spring limiting block corresponding to the spring, and the spring is distributed below the spring limiting block; the lower end of the pressure rod is provided with a pressing net; the immersion-pressing net is made of a 10-20 mesh screen.

8. A nematode sampling separator as claimed in claim 1, wherein: the leaching bottle is detachably connected to the upper part of the separation bottle.

9. A nematode sampling separator as claimed in claim 1, wherein: still include the safety cover, the cover can be dismantled to the safety cover and overlap the outside of separation bottle.

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