CN100534566C - Device and method for extracting chemical composition from plant - Google Patents
Device and method for extracting chemical composition from plant Download PDFInfo
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- CN100534566C CN100534566C CNB2007101345818A CN200710134581A CN100534566C CN 100534566 C CN100534566 C CN 100534566C CN B2007101345818 A CNB2007101345818 A CN B2007101345818A CN 200710134581 A CN200710134581 A CN 200710134581A CN 100534566 C CN100534566 C CN 100534566C
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Abstract
The invention relates to a device used for quickly extracting chemical constituents from plants and a method thereof, which belongs to the field of chemical analysis technology. An extracting device comprises a cylinder, a plunger rod, a filtering screen plate and a faucet, the plunger rod is arranged on the internal upper end of the cylinder, the filtering screen plate is arranged on the internal lower end of the cylinder, the faucet is arranged on the external lower end of the cylinder. Different extracting solvents can be chosen according to the properties of the chemical constituents of the plants, in the condition of the rotating speed of 14,000 to 22,000r/min, high-speed agitation can be carried on for 0.5 to 2min, then the faucet is opened, external pressure is added for filtering, and filter residue is stirred with the solvents for reextraction. The device of the invention gathers the extracting samples and the filtering into a device can reduce the loss of samples in the transferring and extracting processed, at the same time, through adding external pressure for filtering, filtering speed can be improved, so the extracting process is only 10 to 20 minutes, thus achieving the good extracting effect, simple and fast operation and the hign practicality.
Description
Technical field
The present invention relates to the device and method of chemical composition in a kind of rapid extraction plant, belong to the chemical analysis technology field.Be particularly related to plant chemical ingredient residue rapid extraction technology.
Background technology
Extracting chemical composition to be measured from plant sample is the key of analyzing and testing.Used extraction and filter are two independently unit, sample extracts at extraction flask, be transferred to the filtrate receiving flask by filter, the whole leaching process cycle is longer, sample will easily cause object loss to be measured, especially for trace constituent through repeatedly shifting, repeatedly shift to extract and cause losing of object to be analyzed, impact analysis result's the degree of accuracy probably.
Summary of the invention
Technical problem
The objective of the invention is to overcome problems of the prior art, provide a kind of and integrate extraction and filter extraction element simple in structure, with low cost, easy to operate, that extraction efficiency is high and method.
Technical scheme
For achieving the above object, the invention provides a kind of like this device, it comprises cylinder, plunger rod, filtration sieve plate, cock, described plunger rod is positioned at the column body upper end, and is detachable, during sample extraction it extracted from cylinder, insert during filtration in the cylinder, pressurization leaches sample liquid.Described filtration sieve plate is positioned at the column body lower end, and cock is positioned at the outside lower end of cylinder, and the closed position is put in cock during extraction, and open position is put in cock during filtration.
The present invention also provides the method for chemical composition in a kind of rapid extraction plant, and this method comprises the following steps:
(1) testing sample of pulverizing is added in the extraction element provided by the invention;
(2) select the corresponding solvent that extracts according to the character of object to be determined;
(3) high-speed stirred 0.5～2min under 14000～22000r/min rotating speed;
(4) open cock, insert plunger rod pressure filtration to the filtrate collection container;
(5) filter residue repeating step (3)-(4) is 2～3 times, and merging filtrate is treated thickening-purification technology and detection.
Beneficial effect
Adopt the device and method of chemical composition in the rapid extraction plant of the present invention, sample extraction and filtration are combined in the device, can reduce sample in the loss of shifting leaching process; Character according to object to be determined is selected different extraction solvents, can satisfy the different target thing and analyze demand; Simultaneously, filter by external pressurized, improve the rate of filtration, whole leaching process only needs 10～20min.Extraction effect is consistent with conventional method, and the agricultural chemicals rate of recovery can reach 76%～109%, can satisfy the residue detection needs.Therefore, the present invention is applied widely, and is convenient to use, with low cost.
Description of drawings
Fig. 1 is an extraction element structural representation of the present invention.
Among the figure: 1, plunger rod, 2, cylinder, 3, filter sieve plate, 4, cock
Fig. 2 extraction, filtration common method and the invention process effect compare: dotted line is the common method effect, and solid line is the inventive method effect
The specific embodiment
As shown in Figure 1, this extraction element monnolithic case syringe-like is made of cylinder 2, plunger rod 1, the cock 4 of filtering sieve plate 3 and being positioned at the bottom.Cylinder 2 and plunger rod 1 are made by polypropylene material, and volume has 10～100ml as required.Remove upper end plunger rod 1 during extraction, added sample and extracted solvent by cylinder 2 upper port, insert plunger rod 1 during filtration, pressurization is leached sample extracting solution by the outlet of cylinder 2 bottoms.Filter sieve plate 3 and make by the polypropylene fibre filter plate, thickness 2mm, the mesh-structured physicochemical properties on it are stable, can play the effect of filtering and intercepting.Cock 4 is a polytetrafluoroethylmaterial material, closes when extracting sample, opens during filtration.
Serve as to extract detected object with fenvalerate agricultural chemicals in the pears below, further specify specific implementation method of the present invention.Mainly may further comprise the steps:
The first step: remove plunger rod, the closed position is put in cock.
Second step: get pears sample that 5g smashes in extraction column, add the 20ml acetonitrile, mix.
The 3rd step: with refiner homogenate 1min under the 12000r/min rotating speed.
The 4th step: open cock, insert plunger rod, pressurization is filtered to the 100ml Erlenmeyer flask with sample extracting solution.
The 5th step: carefully take off plunger rod, cut-out tap adds the 10ml acetonitrile.
The 6th step: repeat～the four step of the 3rd step 2～3 times, merging filtrate, rotation concentrates after-purification, uses gas chromatographic detection.
Implementation result: see Fig. 2.
Adopt apparatus and method of the present invention, extraction effect is consistent with conventional method, average recovery rate for pyrethrin pesticide in the pears reaches 78.51%～101.59%, relative standard deviation 10.36%～14.23%, the average recovery rate of organophosphorus insecticide is 76.21%～109.75%, relative standard deviation 10.96%～16.58% meets the residue detection requirement.
This device of table 1 is used for extracting pears chrysanthemum ester class, organophosphorus pesticide residue rate of recovery result
Claims (2)
1, a kind of device that extracts chemical composition in the plant, it comprises cylinder, plunger rod, filtration sieve plate, cock, it is characterized in that:
Described plunger rod is positioned at the column body upper end, and is detachable, during sample extraction it extracted from cylinder, inserts during filtration in the cylinder, and pressurization leaches sample liquid; Described filtration sieve plate is positioned at the column body lower end, and cock is positioned at the outside lower end of cylinder, and the closed position is put in cock during extraction, and open position is put in cock during filtration.
2, the described device of claim 1 is used for extracting the method for plant chemical composition, comprises the following steps:
(1) testing sample of pulverizing is added in the described device of claim 1;
(2) select the corresponding solvent that extracts according to the character of object to be determined;
(3) add extraction solvent, high-speed stirred 0.5～2min under 14000～22000r/min rotating speed;
(4) open cock, insert plunger rod pressure filtration to the filtrate collection container;
(5) filter residue repeating step (3)-(4) is 2～3 times, and merging filtrate is treated thickening-purification technology and detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007101345818A CN100534566C (en) | 2007-11-02 | 2007-11-02 | Device and method for extracting chemical composition from plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007101345818A CN100534566C (en) | 2007-11-02 | 2007-11-02 | Device and method for extracting chemical composition from plant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101234263A CN101234263A (en) | 2008-08-06 |
| CN100534566C true CN100534566C (en) | 2009-09-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2007101345818A Expired - Fee Related CN100534566C (en) | 2007-11-02 | 2007-11-02 | Device and method for extracting chemical composition from plant |
Country Status (1)
| Country | Link |
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| CN (1) | CN100534566C (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI467014B (en) * | 2011-09-13 | 2015-01-01 | Taigen Bioscience Corp | A liquid handling plunger for biological sample in a tube |
| CN105203377A (en) * | 2015-09-29 | 2015-12-30 | 齐国良 | Purifying device for pretreatment of Aspergillus flavus and method adopting same |
| CN105641969A (en) * | 2016-01-08 | 2016-06-08 | 严修长 | Traditional Chinese medicine extraction tank |
| CN107497133B (en) * | 2017-07-12 | 2019-09-03 | 上海伽誉生物科技有限公司 | The preparation method of the degeneration-resistant factor HPEs of haematococcus |
| CN110470808A (en) * | 2018-05-11 | 2019-11-19 | 中国石油化工股份有限公司 | Water quality many reference amounts detection device and detection method based on manual injector |
| CN113318475A (en) * | 2021-04-27 | 2021-08-31 | 西藏农牧学院 | Method for separating active ingredients of fomes linnaeus from fomes linnaeus |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2249901Y (en) * | 1995-09-01 | 1997-03-19 | 汪若梅 | Solid extraction collumn |
| JP2004108838A (en) * | 2002-09-17 | 2004-04-08 | Showa Denko Kk | Cartridge column for extracting solid phase |
| CN101028569A (en) * | 2006-03-03 | 2007-09-05 | 中国林业科学研究院资源昆虫研究所 | Fast extraction of plant chemical ingredient |
-
2007
- 2007-11-02 CN CNB2007101345818A patent/CN100534566C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2249901Y (en) * | 1995-09-01 | 1997-03-19 | 汪若梅 | Solid extraction collumn |
| JP2004108838A (en) * | 2002-09-17 | 2004-04-08 | Showa Denko Kk | Cartridge column for extracting solid phase |
| CN101028569A (en) * | 2006-03-03 | 2007-09-05 | 中国林业科学研究院资源昆虫研究所 | Fast extraction of plant chemical ingredient |
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| CN101234263A (en) | 2008-08-06 |
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Granted publication date: 20090902 Termination date: 20101102 |