CN105203377A - Purifying device for pretreatment of Aspergillus flavus and method adopting same - Google Patents
Purifying device for pretreatment of Aspergillus flavus and method adopting same Download PDFInfo
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- CN105203377A CN105203377A CN201510632151.3A CN201510632151A CN105203377A CN 105203377 A CN105203377 A CN 105203377A CN 201510632151 A CN201510632151 A CN 201510632151A CN 105203377 A CN105203377 A CN 105203377A
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Abstract
The invention belongs to the field of pretreatment equipment, and particularly relates to a purifying device for pretreatment of Aspergillus flavus and a method adopting the same. The purifying device comprises a purifying column and a liquid storage pipe, the purifying column is composed of a rubber sealing head and a plastic pipe, diameter of the lower end of the rubber sealing head is 12mm while that of the upper end of the same is 8mm, a liquid passing hole is formed in the center of the rubber sealing head, degreased cotton is filled between the outer side of the liquid passing hole and the inner wall of the rubber sealing head, the upper end of the rubber sealing head is inserted into the plastic pipe, and a leakproof quartz filter element piece, purifying filler and a sealing quartz filter element piece are sequentially arranged in the plastic pipe from bottom to top. The purifying device is simple in structure, low in manufacturing cost, needless of negative pressure suction in the process of purifying and stable in purifying performance, purifying speed can be controlled manually, and purifying effect can meet subsequent detection needs.
Description
Technical field
The invention belongs to pre-processing device field, particularly relate to a kind of aspergillus flavus pre-treatment purification plant and adopt the method for this purification plant.
Background technology
Aflatoxin is the compound that a class chemical constitution is similar, is the derivant of dihydrofuran cumarin, and that Common Toxicity is stronger is B1, B2, G1, G2 etc.Aflatoxin toxicity is extremely strong, when people's intake is large, acute poisoning can occur, occur oxyhepatitis, hemorrhagic necrosis, hepatic cell fattydegeneration and bile duct proliferation.When trace continues to take in, can slow poisoning be caused, growth disorder, cause fibrous lesions, cause proliferation of fibrous tissue.The carcinogenicity of aflatoxin also ranks first, and is one of the strongest known carcinogenic substance at present.Aspergillus flavus is common in soil, animals and plants, various nut, particularly easily pollutes the grain oil products such as peanut, corn, rice, soybean, wheat.
At present, in aspergillus flavus detection method, the decontaminating column used is immune affinity column, the manufacture method more complicated of this decontaminating column, by the impact of immune antiboidy activity, purifying property is unstable, and price is very high, while increasing actual testing cost, poor stability is also for work brings inconvenience.
Summary of the invention
The present invention is directed to the deficiency that above-mentioned prior art exists, provide a kind of structure simple, use efficient aspergillus flavus pre-treatment purification plant and adopt the method for this purification plant.
The technical scheme that the present invention solves the problems of the technologies described above is as follows: a kind of aspergillus flavus pre-treatment purification plant, its special character is, comprise decontaminating column and liquid storage pipe, described decontaminating column is made up of rubber seal head and plastic tube, the lower end diameter of described rubber seal head is 12mm, upper end diameter is 8mm, and described rubber seal head center is provided with liquid-through hole, is filled with absorbent cotton outside described liquid-through hole and between described rubber seal head inwall; The upper end of described rubber seal head is inserted in described plastic tube, is provided with the green chip of leakproof quartz from bottom to up successively, purifies filler and seal quartzy filter element slice in described plastic tube.
On the basis of technique scheme, the present invention can also do following improvement.
Further, described liquid storage pipe is the glass test tube of long 7cm, internal diameter 1cm.
The beneficial effect of above-mentioned further scheme is adopted to be that the internal diameter of liquid storage pipe is slightly less than the diameter of described rubber seal head lower end, improves the sealing between decontaminating column and liquid storage pipe.
Further, described plastics pipe range 15cm, internal diameter is 7.5mm.
The beneficial effect of above-mentioned further scheme is adopted to be that the internal diameter of plastic tube is slightly less than the diameter of described rubber seal head upper end, improves the sealing between rubber seal head and plastic tube.
Further, described purification filler composition is: 50mgPSA, 40mg ketjenblack EC, 50mgC18 reverse phase silica gel filler, 3g sodium chloride, 1g anhydrous sodium acetate and 2g anhydrous sodium sulfate.
The beneficial effect of above-mentioned further scheme is adopted to be utilize the impurity in purification filler removal sample liquid.
Further, described PSA is the ethylene diamine-modified silica filler of N-propyl group, and described C18 reverse phase silica gel filler is the reverse phase silica gel filler that octadecyl is modified.
Adopt the method for above-mentioned aspergillus flavus pre-treatment purification plant, step is as follows:
(1) take 10g, particle diameter be 40 object samples in 50mL centrifuge tube, add 40mL acetonitrile solution, vortex oscillation 1min, ultrasonic extraction 30min, centrifugal 8min under 5000r/min condition;
(2) the supernatant 5mL of step (1) is got in liquid storage pipe, the rubber seal head of decontaminating column is inserted in liquid storage pipe downward vertically, and be depressed into bottom liquid storage pipe, make the solution in liquid storage pipe successively via rubber seal head liquid-through hole, leakproof quartz filter element slice, purify filler and seal quartzy filter element slice upper reaches, and be stored in plastic tube;
(3) from the plastic tube of step (2), take out 2mL solution, add in 50mL flat bottom flask, decompression distillation, use 1mL methanol-eluted fractions, via hole diameter is after 0.22 μm of nylon leaching film filters, and adopts Liquid Chromatography-Tandem Mass Spectrometry combined instrument to detect.
Wherein, described sample is peanut, corn, rice, soybean or wheat.
Described acetonitrile solution is the volume ratio of acetonitrile and water is 8:2.
The invention has the beneficial effects as follows: purification plant structure of the present invention is simple, and cheap for manufacturing cost, purification process is without the need to taking out negative pressure, and manually can control purification speed, purifying property is stablized, and clean-up effect can meet follow-up detection demand.
Accompanying drawing explanation
Fig. 1 is the structural representation of decontaminating column of the present invention;
In figure, 1, plastic tube; 2, quartzy filter element slice is sealed; 3, filler is purified; 4, the green chip of leakproof quartz; 5, absorbent cotton; 6, liquid-through hole; 7, rubber seal head.
Embodiment
Be described principle of the present invention and feature below in conjunction with example, example, only for explaining the present invention, is not intended to limit scope of the present invention.
Embodiment 1
A kind of aspergillus flavus pre-treatment purification plant, its special character is, comprise decontaminating column and liquid storage pipe, described decontaminating column is made up of rubber seal 7 and plastic tube 1, the lower end diameter of described rubber seal 7 is 12mm, upper end diameter is 8mm, and described rubber seal 7 center are provided with liquid-through hole 6, is filled with absorbent cotton 5 outside described liquid-through hole 6 and between described rubber seal 7 inwall; The upper end of described rubber seal 7 is inserted in described plastic tube 1, is provided with the green chip 4 of leakproof quartz, purification filler 3 from bottom to up successively and seals quartzy filter element slice 2 in described plastic tube 1.
Wherein, described liquid storage pipe is the glass test tube of long 7cm, internal diameter 1cm.
Described plastics pipe range 15cm, internal diameter is 7.5mm.
Described purification filler composition is: reverse phase silica gel filler, 3g sodium chloride, 1g anhydrous sodium acetate and 2g anhydrous sodium sulfate that the ethylene diamine-modified silica filler of 50mgN-propyl group, 40mg ketjenblack EC, 50mg octadecyl are modified.
Adopt the method for above-mentioned aspergillus flavus pre-treatment purification plant, step is as follows:
(1) take 10g, particle diameter is that 40 object samples are in 50mL centrifuge tube, add 40mL acetonitrile solution (acetonitrile: water=8:2 (v/v)), vortex oscillation 1min, ultrasonic extraction 30min, centrifugal 8min under 5000r/min condition;
(2) the supernatant 5mL of step (1) is got in liquid storage pipe, the rubber seal head of decontaminating column is inserted in liquid storage pipe downward vertically, and be depressed into bottom liquid storage pipe, make the solution in liquid storage pipe successively via rubber seal head liquid-through hole, leakproof quartz filter element slice, purify filler and seal quartzy filter element slice upper reaches, and be stored in plastic tube;
(3) from the plastic tube of step (2), take out 2mL solution, add in 50mL flat bottom flask, decompression distillation, use 1mL methanol-eluted fractions, via hole diameter is after 0.22 μm of nylon leaching film filters, and adopts Liquid Chromatography-Tandem Mass Spectrometry combined instrument to detect.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. an aspergillus flavus pre-treatment purification plant, it is characterized in that, comprise decontaminating column and liquid storage pipe, described decontaminating column is made up of rubber seal head and plastic tube, the lower end diameter of described rubber seal head is 12mm, upper end diameter is 8mm, and described rubber seal head center is provided with liquid-through hole, is filled with absorbent cotton outside described liquid-through hole and between described rubber seal head inwall; The upper end of described rubber seal head is inserted in described plastic tube, is provided with the green chip of leakproof quartz from bottom to up successively, purifies filler and seal quartzy filter element slice in described plastic tube.
2. purification plant according to claim 1, is characterized in that, described liquid storage pipe is the glass test tube of long 7cm, internal diameter 1cm.
3. purification plant according to claim 1, is characterized in that, described plastics pipe range 15cm, internal diameter is 7.5mm.
4. purification plant according to claim 1, is characterized in that, described purification filler composition is: 50mgPSA, 40mg ketjenblack EC, 50mgC18 reverse phase silica gel filler, 3g sodium chloride, 1g anhydrous sodium acetate and 2g anhydrous sodium sulfate.
5. purification plant according to claim 4, is characterized in that, described PSA is the ethylene diamine-modified silica filler of N-propyl group, and described C18 reverse phase silica gel filler is the reverse phase silica gel filler that octadecyl is modified.
6. adopt a method for aspergillus flavus pre-treatment purification plant, it is characterized in that, step is as follows:
(1) take 10g, particle diameter be 40 object samples in 50mL centrifuge tube, add 40mL acetonitrile solution, vortex oscillation 1min, ultrasonic extraction 30min, centrifugal 8min under 5000r/min condition;
(2) the supernatant 5mL of step (1) is got in liquid storage pipe, the rubber seal head of decontaminating column is inserted in liquid storage pipe downward vertically, and be depressed into bottom liquid storage pipe, make the solution in liquid storage pipe successively via rubber seal head liquid-through hole, leakproof quartz filter element slice, purify filler and seal quartzy filter element slice upper reaches, and be stored in plastic tube;
(3) from the plastic tube of step (2), take out 2mL solution, add in 50mL flat bottom flask, decompression distillation, use 1mL methanol-eluted fractions, via hole diameter is after 0.22 μm of nylon leaching film filters, and adopts Liquid Chromatography-Tandem Mass Spectrometry combined instrument to detect.
7. method according to claim 6, is characterized in that, described sample is peanut, corn, rice, soybean or wheat.
8. method according to claim 6, is characterized in that, described acetonitrile solution is the volume ratio of acetonitrile and water is 8:2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510632151.3A CN105203377A (en) | 2015-09-29 | 2015-09-29 | Purifying device for pretreatment of Aspergillus flavus and method adopting same |
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| CN201510632151.3A CN105203377A (en) | 2015-09-29 | 2015-09-29 | Purifying device for pretreatment of Aspergillus flavus and method adopting same |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107607659A (en) * | 2017-11-10 | 2018-01-19 | 贵州大学 | A kind of improved gas chromatograph |
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| CN101234263A (en) * | 2007-11-02 | 2008-08-06 | 江苏省农业科学院 | Device and method for extracting chemical composition from plant |
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2015
- 2015-09-29 CN CN201510632151.3A patent/CN105203377A/en active Pending
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| CN101234263A (en) * | 2007-11-02 | 2008-08-06 | 江苏省农业科学院 | Device and method for extracting chemical composition from plant |
| CN101954202A (en) * | 2009-07-16 | 2011-01-26 | 中国科学院化学研究所 | Constant-pressure closed ascending chromatographic column device and application method thereof |
| CN103185756A (en) * | 2011-12-28 | 2013-07-03 | 中国石油天然气股份有限公司 | Sample pretreatment method for analysis of anions in alkanolamine solution |
| CN203044027U (en) * | 2012-12-24 | 2013-07-10 | 北京农产品质量检测与农田环境监测技术研究中心 | Suction pipe for fast detecting pesticide residue |
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| YONGGANG LI ET AL.: "Ultra-high performance liquid chromatography tandem mass spectrometry for simultaneous analysis of aflatoxins B1, G1, B2, G2, zearalenone and its metabolites in eggs using a QuEChERS-based extraction procedure", 《ANALYTICAL METHODS》 * |
| 小E哥哥7379 上传: "上海泉岛公司", 《百度文库：HTTPS://WENKU.BAIDU.COM/VIEW/1855507701F69E314332945C.HTML》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107607659A (en) * | 2017-11-10 | 2018-01-19 | 贵州大学 | A kind of improved gas chromatograph |
| CN107607659B (en) * | 2017-11-10 | 2024-02-06 | 贵州大学 | Improved meteorological chromatograph |
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Application publication date: 20151230 |