CN211652547U

CN211652547U - Fluorescent probe detector for simultaneously detecting hydrogen sulfide and nitric oxide

Info

Publication number: CN211652547U
Application number: CN202020242418.4U
Authority: CN
Inventors: 许贯虹; 姚瑶; 李胜男; 陈佳婧; 贺采灵; 黄琼叶; 胡琴
Original assignee: Nanjing Medical University
Current assignee: Nanjing University; Nanjing Medical University
Priority date: 2020-03-03
Filing date: 2020-03-03
Publication date: 2020-10-09
Anticipated expiration: 2030-03-03

Abstract

The utility model relates to a fluorescent probe detector for simultaneously detecting hydrogen sulfide and nitric oxide, which comprises a box cover and a box body which are connected by a buckle-type groove, wherein the box cover is provided with a notch for conveniently placing a test strip; the box body covers the black background plate, a charging type power supply plate is arranged between the box body and the black background plate, the box cover is of a cavity structure, and ultraviolet lamp beads are arranged on the periphery of the inner side of the box cover; after the fluorescent probe strip is placed in the incision, it is placed on a black background plate. The two ends of the fluorescent probe test strip are respectively a hydrogen sulfide sampling area and a nitric oxide sampling area, and the middle is a blank control area. When the test paper box is used, the ultraviolet lamp is turned on, a solution containing a sample is dripped onto the fluorescent probe test paper strip, the test paper strip is placed through the notch of the box cover, the sample molecules selectively react with the fluorescent probe loaded on the test paper, the test paper emits fluorescence of different colors under a fluorescent lamp, the content of the observed result can be judged by contrasting with a standard color card, the operation is rapid, the test paper box is simple and practical, the result is accurate, and the reproducibility is good.

Description

Fluorescent probe detector for simultaneously detecting hydrogen sulfide and nitric oxide

Technical Field

The utility model relates to a harmful gas check out test set field, concretely relates to fluorescence detection appearance of hydrogen sulfide and nitric oxide concentration in simultaneous detection environment.

Background

The hydrogen sulfide is a flammable and dangerous chemical substance, can form an explosive mixture when being mixed with air, and can cause explosive combustion when meeting open fire and high heat. Low concentrations of hydrogen sulfide have an effect on the eyes, respiratory system and central nervous system. When the concentration of hydrogen sulfide in the environment rises, the absorption of a small amount of high-concentration hydrogen sulfide can cause hemoglobin to lose oxygen carrying capacity, so that oxygen deficiency of tissues is caused, death is caused in severe cases, and meanwhile, the skin and mucous membranes have strong stimulation and corrosion effects, so that coagulation necrosis of the tissues is generated.

Nitric oxide is less reactive, but is readily oxidized in air to nitrogen dioxide, which is highly corrosive and toxic. When it enters the respiratory tract, it produces strong irritation and erosion to the lung tissue, which can cause pulmonary edema. Meanwhile, the nitric oxide has strong oxidizing property, and can easily catch fire and burn when being contacted with inflammable substances and organic substances.

The limit value of the hydrogen sulfide content in the national standard is 30mg/m³The limiting value of the first-grade standard annual average concentration value of nitrogen dioxide is 0.04mg/m³. In order to detect the quality of environmental gas, the concentration of hydrogen sulfide and nitric oxide in the environment needs to be detected, and along with the improvement of the requirement of people on the environmental quality, strong demands on various environment-friendly detection instruments begin to appear.

At present, a detection instrument for detecting environment harmful gas in the market is expensive, needs to be operated by professional personnel, and is not suitable for ordinary consumers to use in daily life. Although the appearance of the detection reagent alleviates this situation to some extent, there are problems of complicated operation steps, small and insensitive detection concentration range, and unstable performance. Meanwhile, detection test strips for detecting harmful gases exist in the market, but the detection limit is low, the detection range is narrow, and only a certain type of single gas can be detected.

Disclosure of Invention

In order to overcome the defects of the prior art, the utility model aims to provide a fluorescence probe detector for simultaneously detecting hydrogen sulfide and nitric oxide, which has the advantages of reliable result, good reproducibility, convenient use and low cost.

The utility model provides a detect hydrogen sulfide and nitric oxide's fluorescence probe detector simultaneously, includes upper and lower complex lid and box body, the lid with the box body is connected through buckle formula recess, is equipped with the incision of being convenient for place the test paper strip on the lid.

The groove is arranged on the edge of the box body.

According to the fluorescent probe detector for simultaneously detecting hydrogen sulfide and nitric oxide, the box body is covered with the black background plate, the rechargeable power supply plate is arranged between the box body and the black background plate, and the shape of the black background plate is matched with that of the box body; the box body is also provided with a hole groove and a switch which are convenient for the switching on of the charging type power supply.

In the fluorescent probe detector for simultaneously detecting hydrogen sulfide and nitric oxide, the box cover is of a cavity structure, and ultraviolet lamp beads are arranged on the periphery of the inner side of the box cover; the notch on the box cover is rectangular, and the fluorescent probe test strip is placed on the black background plate after being placed in the notch.

In the fluorescent probe detector for simultaneously detecting hydrogen sulfide and nitric oxide, the fluorescent probe test strip comprises a handle and a sample application area; the areas of the sampling area at the two ends are respectively a hydrogen sulfide sampling area and a nitric oxide sampling area, and the middle area is a blank comparison area.

According to the fluorescent probe detector for simultaneously detecting hydrogen sulfide and nitric oxide, the three areas of the sampling area are provided with the corresponding test paper, and the hydrogen sulfide sampling area and the nitric oxide sampling area at the two ends are provided with the fluorescent probes in a printing mode. The fluorescent probe of the sample application area comprises 3-azidophthalimide for detecting hydrogen sulfide and 4- ((3-amino-2-naphthyl) aminomethyl) benzoic acid (DAN-1) for detecting nitric oxide.

Has the advantages that:

1. the fluorescent probe detector can simultaneously detect the concentration of hydrogen sulfide and nitric oxide, and overcomes the problem that the existing fluorescent probe detector can only detect the concentration of single gas at one time.

When the test paper is used, the solution containing the sample is only required to be dropped on the test paper, the result can be obtained in a short time, the operation is quick, and the result is accurate.

Drawings

FIG. 1 is a schematic structural diagram of a fluorescence probe detector for simultaneously detecting hydrogen sulfide and nitric oxide according to the present invention;

FIG. 2 is a schematic structural diagram of a fluorescent probe strip.

The kit comprises a kit cover-2, a kit body-3, a black background plate-4, a groove-5, a hole groove-6, a switch-7, an ultraviolet lamp bead-8, a notch-9, a fluorescent probe test strip-10, a handle-11, a sampling area 12 and a blank control area 13.

Detailed Description

In order to make the technical problem solved by the present invention, the technical solution adopted by the present invention and the technical effect achieved by the present invention clearer, the following description further illustrates the technology of the present invention by combining the accompanying drawings and by means of the specific implementation manner.

Example 1

FIG. 1 is a schematic structural diagram of a fluorescence probe detector for simultaneously detecting hydrogen sulfide and nitric oxide according to the present invention; referring to fig. 1, a fluorescent probe detector 1 for simultaneously detecting hydrogen sulfide and nitric oxide includes a box cover 2 and a box body 3 which are vertically matched; in the embodiment, the box cover 2 is a cuboid with irregular four corners; the box cover 2 is connected with the box body 3 through a clamping groove 5.

The box cover 2 is of a cavity structure, and ultraviolet lamp beads 8 are arranged on the periphery of the inner side of the box cover. The box body 3 is covered with a black background plate 4, the shape of the black background plate 4 is matched with that of the box body 3, and a rechargeable power panel is arranged between the box body 3 and the black background plate 4.

The edge of the box body 3 is provided with a groove 5 which can be inserted and clamped by the box cover 2, and the box body 3 is also provided with a hole groove 6 and a switch 7 which are convenient for the charging type power supply to be switched on. The box cover 2 is provided with a notch 9 for placing the test paper, the notch 9 is rectangular, and the fluorescent probe test paper is placed on the black background plate 4 after being placed from the notch 9.

FIG. 2 is a schematic structural diagram of a fluorescent probe strip. Referring to FIG. 2, the fluorescent probe strip 11 includes a handle 11 and a sample application region 12; the areas of the sampling area 12 at the two ends are a hydrogen sulfide sampling area and a nitric oxide sampling area respectively, the middle area is a blank comparison area 13, three areas are filled with test paper corresponding to each other, and the hydrogen sulfide sampling area and the nitric oxide sampling area 13 at the two ends are loaded with fluorescent probes in a printing mode. The fluorescent probe comprises 3-azidophthalimide for detecting hydrogen sulfide and 4- ((3-amino-2-naphthyl) aminomethyl) benzoic acid (DAN-1) for detecting nitric oxide (hydrogen sulfide probe molecule design reference: Tetrahedron Letters 55 (2014) 5566-type 5569; nitric oxide probe molecule reference: Biological & pharmaceutical bulletin, 1997, 20(12), 1229-type 1232).

When the fluorescent probe detector is used, a rechargeable power panel of the fluorescent probe detector is electrified, an ultraviolet lamp is turned on, a solution containing a sample is dripped onto a fluorescent probe test strip, the fluorescent probe test strip is placed on a black background plate 4 in the detector from a notch 9, sample molecules selectively react with a fluorescent probe loaded on the test strip, the test strip emits fluorescence of different colors under the fluorescence, and the content of the test strip can be judged according to an observation result by contrasting a standard color card.

The above description is only for the preferred embodiment of the present invention, and for those skilled in the art, there are variations on the detailed description and the application scope according to the idea of the present invention, and the content of the description should not be construed as a limitation to the present invention.

Claims

1. The utility model provides a detect fluorescence probe detector of hydrogen sulfide and nitric oxide simultaneously, its characterized in that, includes upper and lower complex lid and box body, the lid with the box body is connected through buckle formula recess, is equipped with the incision of being convenient for place the test paper strip on the lid.

2. The fluorescence probe detector of claim 1, wherein the notch is disposed at the edge of the case.

3. The fluorescence probe detector for simultaneously detecting hydrogen sulfide and nitric oxide as claimed in claim 1, wherein the box body is covered with a black background plate, a rechargeable power supply plate is placed between the box body and the black background plate, and the shape of the black background plate is matched with that of the box body; the box body is also provided with a hole groove and a switch which are convenient for the switching on of the charging type power supply.

4. The fluorescence probe detector for simultaneously detecting hydrogen sulfide and nitric oxide according to claim 1, wherein the box cover has a cavity structure, and ultraviolet lamp beads are arranged around the inner side of the box cover; the notch on the box cover is rectangular, and the fluorescent probe test strip is placed on the black background plate after being placed in the notch.

5. The fluorescence probe detector of claim 1 or 4, wherein the fluorescence probe strip comprises a handle and a sample application region; the areas of the sampling area at the two ends are respectively a hydrogen sulfide sampling area and a nitric oxide sampling area, and the middle area is a blank comparison area.

6. The fluorescence probe detector for simultaneously detecting hydrogen sulfide and nitric oxide according to claim 5, wherein three areas of the spotting area are provided with corresponding test paper, and the hydrogen sulfide spotting area and the nitric oxide spotting area at two ends are loaded with fluorescence probes by printing.