CN211611319U

CN211611319U - Remote control's flexible electron chitosan of pulse electrical stimulation is applied ointment or plaster

Info

Publication number: CN211611319U
Application number: CN201921519990.4U
Authority: CN
Inventors: 谈伟强; 王晓凤; 赵婉意; 楼栋; 方三华; 陈剑
Original assignee: Zhejiang University ZJU
Current assignee: Zhejiang University ZJU
Priority date: 2019-09-12
Filing date: 2019-09-12
Publication date: 2020-10-02
Anticipated expiration: 2029-09-12

Abstract

The utility model provides a remote control's flexible electron chitosan of pulse electro-stimulation is applied ointment or plaster comprises terminal and the flexible electron chitosan of pulse electro-stimulation, the terminal includes application and the terminal communication device that the flexible electron chitosan of pulse electro-stimulation was applied ointment or plaster. The pulse electrical stimulation flexible electronic chitosan application comprises a wireless communication device, a back lining layer, a pulse electrical stimulation device, a protective film, an early warning device, a drug cushion layer and release paper. The utility model discloses can acquire patient's surface of a wound treatment information in real time, effectively mobilize the communication and the supervision of chronic surface of a wound regularity treatment between the doctor-patient, accomplish the effective treatment of long-term law. The utility model discloses the early warning before the electro photoluminescence has the guard action to patient's physical and mental health. The common electrical stimulation equipment needs to be used independently, and has large volume, difficult movement and inconvenient operation. And the utility model discloses a device is small, convenient to carry, and has remote control's function, the very big facility of bringing for patient's life.

Description

Remote control's flexible electron chitosan of pulse electrical stimulation is applied ointment or plaster

Technical Field

The utility model belongs to medical application, concretely relates to remote control's flexible electron chitosan of pulse electrical stimulation is applied ointment or plaster.

Background

The difficult-to-heal wound surface of diabetes is a big problem faced by the global health care system. The chronic wound of diabetes has the characteristics of long course of disease, susceptibility to infection, more complications, high treatment cost and the like, and needs long-term, regular and personalized treatment. However, under the pace of modern compact and fast-paced life, frequent and expensive treatment causes patients with chronic wounds of diabetes to feel tired and desperate, and many people often cannot follow medical advice, so that the wounds are worsened and finally even endanger life.

The clinical treatment of the diabetic wound is mainly debridement and wound dressing covering. The wound dressing not only can protect the wound from being damaged by environmental irritants, but also provides an optimal microenvironment so that the tissues of the wound can be continuously reconstructed. The chitosan has the advantages of good biocompatibility, degradability, no toxicity, antibiosis and the like, and the application potential in the aspect of dressing is continuously explored. The chitosan with high and low molecular weight has different pharmacological effects, such as the antibacterial effect of gram-positive bacteria staphylococcus aureus is gradually enhanced along with the increase of the molecular weight of the chitosan. In the case of gram-negative bacteria, the antibacterial action is gradually increased with the decrease in molecular weight. And the low molecular weight chitosan has relatively better moisturizing performance. Therefore, the chitosan with different molecular weights is mixed to prepare the chitosan mixture, and the performances of the chitosan can be fully exerted. Nevertheless, chitosan dressings are less effective for the treatment of chronic wounds of diabetes. In recent years, people find that the pulse electrical stimulation has a more definite curative effect on treating chronic wounds. Because it can simulate endogenous damage current, it can induce the migration and proliferation of repairing cell and stimulate the secretion of several growth factors, so that it can promote the healing of wound surface. The treatment of diabetic wounds by electrical stimulation has gradually entered the clinic.

With the rapid development of computer science and information technology, remote medicine is driving the development of modern medical technology to wider and deeper fields exceeding 'space' and 'time' with astonishing speed and influence, developing new modes and new boundaries of medical services, breaking the limits of traditional medicine in 'environment', 'place' and other aspects, and playing a greater and greater role in improving the quality of life of people. The mobile phone and the computer are high-tech products, bring much convenience to life, and are necessities of life.

Most of the existing electrical stimulation devices are used independently, and have large volume and difficult movement, so that patients are very inconvenient to use. The remote control is not possible, and the treatment of the wound surface is difficult to keep regular due to the long course of disease of the diabetic wound surface patient. In addition, for the patients with diabetic wounds, the psychological construction before the electrical stimulation is very important for the physical and mental health of the patients. Therefore, the pulse electrical stimulation flexible electronic chitosan application capable of being controlled remotely is designed, and the pulse electrical stimulation device 7 is made of flexible electronic materials and is convenient to use. The preparation of flexible electronic material adopts "flexible printed circuit board" technique, uses polyimide as the substrate, adopts micro-nano processing technique, designs the special performance of can stretching of meandering structure in order to realize the device to promote the adaptability of device to the curved surface, reinforcing and the laminating degree of skin. In addition, family members and doctors of the patient can remotely carry out electrostimulation treatment on the wound of the patient, and the electric stimulation treatment device has the functions of forecasting before electrostimulation and reminding at fixed time by electrostimulation time, so that the healing of the wound is better promoted, and the medical experience of the patient is improved.

Disclosure of Invention

The to-be-solved technical problem of the utility model is to current electro photoluminescence defect, apply ointment or plaster wireless communication technique, electro photoluminescence, flexible printed circuit board technique and chitosan and combine together, provide humanized, controllable remote control's pulse electro photoluminescence flexible electron chitosan and apply ointment or plaster.

In order to solve the technical problem, the utility model discloses a technical scheme is: a remote-control flexible electronic chitosan application electrically stimulated by pulses comprises a terminal 1 and a flexible electronic chitosan application 2 electrically stimulated by pulses.

The terminal 1 can be a mobile phone or a computer terminal and comprises an application program 3 of pulse electrical stimulation flexible electronic chitosan application and a terminal communication device 4, the application program of the pulse electrical stimulation flexible electronic chitosan application comprises a patient information module 3-1, an electrical stimulation parameter module 3-2 and a communication module 3-3, and the patient information module 3-1 is used for providing basic information of a patient, such as information of age, sex, past history and the like. The electrical stimulation parameter module 3-2 can set electrical stimulation parameters and directly issue commands to the pulse electrical stimulation flexible electronic chitosan application 2 of the patient, and the communication module 3-3 can send information to the mobile phone terminal of the patient, the family members of the patient and the mobile phone or the computer terminal of the doctor.

The shape of the pulse electrical stimulation flexible electronic chitosan application 2 can be various shapes, such as a square, a circle, a triangle and the like, and the shape and the size of the application are determined according to the shape of a wound.

The pulse electrical stimulation flexible electronic chitosan application 2 comprises a wireless communication device 5, a back lining layer 6, a pulse electrical stimulation device 7, a protective film 8, an early warning device 9, a medicine cushion layer 10 and release paper 11. The pulse electrical stimulation flexible electronic chitosan application 2 is sequentially provided with a back lining layer 6, a pulse electrical stimulation device 7, two layers of protective films 8, a medicine cushion layer 10 and release paper 11 from the uppermost layer to the lowermost layer, wherein the wireless communication device 5 and the early warning device 9 are simultaneously positioned between the two layers of protective films 8.

The pulse electrical stimulation device 7 is made of flexible electronic materials and can emit pulse electricity of 30-150V, 50-100 mus and 80-105 pps. The preparation of flexible electronic material adopts "flexible printed circuit board" technique, uses polyimide as the substrate, designs special sinuous structure in order to realize the tensile property of device to promote the adaptability of device to the curved surface, reinforcing and the laminating degree of skin, its preparation adopts micro-nano processing technique. The pulse electrical stimulation device 7 comprises a flexible substrate 7-1, a battery 7-2 (a button battery or a flexible film battery), a pulse switch circuit 7-3 (consisting of a direct current-direct current boosting module 7-4, a pulse modulation module 7-5 and a circuit switch 7-6), a current sensor 7-7, a lead 7-8, a positive electrode 7-9 and a negative electrode 7-10; wherein the sensor is a current sensor, and the electrodes comprise peripheral positive electrodes 7-9 and central negative electrodes 7-10. After the pulse electrical stimulation device 7 is manufactured, waterproof glue of a circuit board is sprayed, and short circuit caused by water infiltration is prevented.

The pulse electrical stimulation device 7 is positioned in the center of the backing layer 6, and the peripheral positive electrodes 7-9 and the central negative electrodes 7-10 penetrate through the two protective films 8 and the medicine pad layer 10. The protection film 8 has two-layerly, and upper protection film 8 is paintd medical adhesive and is linked to each other with pulse electrical stimulation device 7, and lower floor's protection film 8 is paintd medical adhesive and is linked to each other with medicine bed course 10, puts into between wireless communication device 5 and the early warning device 9 between two-layer protection film 8, and the following of medicine bed course 10 is glued with from type paper 11 and is even.

The protective film 8 is an insulating material, and may be a high temperature resistant polyester film (PET film), an electrical insulating film, etc., and the protective film 8 may prevent water of the drug pad layer 10 from permeating the pulse electro-stimulation device 7, the early warning device 9, and the wireless communication device 5, and may prevent a short circuit of a circuit due to the insulating material.

The early warning device 9 is an electronic alarm and is connected with the sensors 7-7 through leads.

The medicinal pad layer 10 is a medical material with adsorption capacity, and can be absorbent cotton, medical gauze, medical sponge, medical cotton pad, medical cotton spunlace and the like; the chitosan mixture is adsorbed on the drug cushion layer 10 and is uniformly distributed; the chitosan mixture is prepared by mixing high-molecular weight chitosan and low-molecular weight chitosan according to the weight ratio of 1: 4-4: 1, the lower surface of the medicinal pad layer 10 is a mixture layer of vaseline and paraffin oil, and the vaseline and the paraffin oil are mixed according to the weight ratio of 1: 2-2: 1.

The back lining layer 6 is self-adhesive air-permeable polyurethane film, PU film, non-woven fabric and the like, and has viscosity, the back lining layer 6 is glued with the pulse electrical stimulation device 7, the medicine cushion layer 10 and the peripheral exposed part of the release paper 11, and each part is reinforced, the back lining layer 6 and the glue of the release paper 11 are connected, so that the whole application is integrated. The peripheral exposed part refers to the part of the periphery of each layer which is not glued with other layers but is coated with glue.

The terminal communication device 4 and the wireless communication device 5 may be bluetooth communication devices or wireless communication devices.

The terminal 1 comprises a mobile phone terminal of a patient and a mobile phone or a computer terminal of an attending doctor, and the patient and the doctor can simultaneously obtain information of wound treatment, including preset electrical stimulation time and current magnitude, actual electrical stimulation time and current magnitude and the like.

The utility model has the advantages of it is following: 1) can obtain the effective information of the wound treatment of the patient in real time. 2) Effectively mobilize the supervision of doctors and patients' families on the regular treatment of the chronic wound of the diabetic patients, and really realize the regular treatment for a long time. 3) The early warning before the electrical stimulation has a protective effect on the physical and mental health of the diabetic patient. 4) As for the common electrical stimulation equipment, the device needs to be used independently, has large volume and difficult movement, and is very inconvenient for patients to use. And the utility model discloses a pulse electrical stimulation flexible electron device is small, convenient to carry, and has remote control's function, the very big facility of bringing for patient's life.

Drawings

Fig. 1 is a schematic diagram of a remotely controlled pulsed electrical stimulation flexible electronic chitosan application.

Figure 2 is a control schematic diagram of a remotely controlled pulsed electrical stimulation flexible electronic chitosan application.

Fig. 3 is a front view of a remotely controlled pulsed electrical stimulation device for pulsed electrical stimulation of a flexible electronic chitosan application.

Fig. 4 is a functional schematic diagram of the application program of the pulse electrical stimulation flexible electronic chitosan application of the present invention.

FIG. 5 is a comparative graph showing wound healing of the wound surface of diabetic rats to which the single-phase high-voltage pulse electrostimulation chitosan patch of this example 9 was applied.

FIG. 6 is the area and healing rate statistics of the wound surface of diabetic rats to which the single-phase high-voltage pulse electrostimulation chitosan patch of this example 9 was applied.

FIG. 7 is a comparison graph of wound healing promotion in diabetic rats by six groups of electrical parameters of the chitosan patch (surface electrode) for pulsed stimulation in different electrical parameters in the present example 10.

FIG. 8 is a chart showing statistics of wound healing rates of diabetic rats promoted by six groups of electrical parameters of the chitosan patch (surface electrode) for electrical stimulation.

FIG. 9 is a chart showing statistics of the healing rate and the healing days of wound wounds of diabetic rats promoted by six groups of the pulsed electrical stimulation chitosan application (surface electrode) with different electrical parameters in the example 10.

FIG. 10 is a comparison of six groups of electrical parameters of the present example 11 of electrical stimulation with chitosan patch (inserted minimally invasive electrodes) to promote wound healing in diabetic rats.

FIG. 11 is a chart showing statistics of wound healing rates of diabetic rats promoted by six groups of electrical parameters of the pulsed stimulation chitosan patch (inserted minimally invasive electrodes) in the example 11.

FIG. 12 is a chart showing statistics of six groups of electrical parameters of the pulsed electrical stimulation chitosan patch (inserted minimally invasive electrodes) in the present example 11 for promoting the healing rate of the wound surface of the diabetic rat and the healing days.

FIG. 13 shows the scratching test and the Transwell pattern of the HUVEC cells of example 12.

FIG. 14 shows the results of Western blotting and ELISA adsorption experiments in this example 13.

Description of the numbering

1 terminal 2 pulse electrical stimulation flexible electronic chitosan application

Application program 4 terminal communication device with 3-pulse electrical stimulation flexible electronic chitosan application

5 backing layer of wireless communication device 6

7 pulse electro photoluminescence device 8 protection film

9 early warning device 10 medicine cushion layer

11 release paper

3-1 patient information module and 3-2 electrical stimulation parameter module

3-3 communication module

7-1 Flexible substrate 7-2 Battery

7-3 pulse switch circuit 7-4 DC-DC boost module

7-5 pulse modulation module 7-6 circuit switch

7-7 current sensor 7-8 conducting wire

7-9 positive electrode 7-10 negative electrode.

Detailed Description

The invention will be further explained with reference to the drawings and the specific embodiments. The scope of protection of the present invention is not limited by the embodiments. It will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the following claims.

Example 1

Referring to fig. 1-4, a remote-controlled pulsed electrical stimulation flexible electronic chitosan application comprises a terminal 1 and a pulsed electrical stimulation flexible electronic chitosan application 2.

Referring to fig. 1, since the lengths and widths of the respective layers are different, after the adhesive bonding to the upper and lower layers, the relatively long or wide layer has a periphery (periphery exposed by the adhesive) which is not bonded but coated by the adhesive, the backing layer 6 is made of self-adhesive breathable polyurethane film, PU film, non-woven fabric, etc., which has its own viscosity, and the backing layer 6 is bonded to the pulse electrical stimulation device 7, the medicated pad layer 10, and the peripheral exposed portion of the release paper 11, so as to reinforce the bonding of each portion to the backing layer 6 and the release paper 11, thereby integrating the whole application. The peripheral exposed part refers to the part of the periphery of each layer which is not glued with other layers but is coated with glue.

The exposed parts of the pulse electrical stimulation device 7, the medicine cushion layer 10 and the release paper 11 are coated with medical adhesive at the same time, and the adhesion between each part and the back lining layer 6 is reinforced. The backing layer 6 is the same size as the release paper 11.Upper layer ofThe size of the protective film 8 is suitable for the pulse electrical stimulation device 7,lower layerThe size of the protective film 8 is matched with that of the medicine cushion layer 10.

The terminal 1 comprises a mobile phone terminal of a patient and a mobile phone or a computer terminal of an attending doctor, and the patient and the doctor can simultaneously obtain information of wound treatment, including preset electrical stimulation time and current magnitude and actual electrical stimulation time and current magnitude.

EXAMPLE 2A remotely controlled flexible electronic chitosan patch for electrical pulse stimulation (circular; electrical stimulation parameters: 30V,100pps, 100. mu.s; 3% chitosan mixture; weight ratio of vaseline to paraffin oil 1: 2; patient controlled by mobile phone)

Adding 1.5g of high molecular weight chitosan (molecular weight 100 ten thousand) and 1.5g of low molecular weight chitosan (molecular weight 5 thousand) into 97g of 1% acetic acid solution, stirring uniformly to obtain 3% chitosan mixed solution, filtering and sterilizing; and (3) immersing the medical sponge into the chitosan mixed solution for 24 hours, taking out the medical sponge, and drying the medical sponge in a drying oven at the temperature of 60-65 ℃ until water does not drip, thus obtaining the medical sponge containing 3% of chitosan mixed solution. Coating vaseline and paraffin oil mixture (weight ratio of vaseline to paraffin oil is 1:2, and sterilizing with high pressure steam) on the lower part of the dried medical sponge to obtain medicinal pad layer 10.

The early warning device 9 (an electronic alarm connected with a current sensor through a lead) and the wireless communication device 5 (a patch type DTU module E16) are connected with the pulse electrical stimulation device 7 and positioned between the two layers of protective films 8 (electrical insulating films).

The pulse electrical stimulation device 7 is arranged above the early warning device 9 and the wireless communication device 5, the pulse electrical stimulation device 7 is made of flexible electronic materials and comprises a flexible substrate 7-1, a battery 7-2 (a button battery), a pulse switch circuit 7-3 (composed of a direct current-direct current boosting module 7-4, a pulse modulation module 7-5 and a circuit switch 7-6), a current sensor 7-7, a positive electrode 7-9 and a negative electrode 7-10, and the above structures except the flexible substrate 7-1 are connected through a lead 7-8. The electrodes of the pulsed electrical stimulation device 7 are gold electrodes comprising peripheral positive electrodes 7-9 and central negative electrodes 7-10, all passing through a protective film 8 (electrical insulation film) and a drug pad layer 10. The pulse electrical stimulation device 7 can send out 30V,100pps and 100 mus pulse electrical stimulation.

The back lining layer 6 is self-adhesive air-permeable polyurethane film, which has viscosity and can be bonded with the exposed parts of the lower pulse electric stimulation device 7, the medicine cushion layer 10 and the release paper 11 to form a whole. The exposed parts of the pulse electrical stimulation device 7, the medicine cushion layer 10 and the release paper 11 are coated with medical adhesive at the same time, so that the adhesion between each part and the back lining layer 6 is reinforced. The pulsed electrical stimulation device 7 is located in the center of the backing layer 6, and all electrodes pass through the two protective films 8 and the drug pad layer 10. The wireless communication device 5 and the early warning device 9 are positioned between two layers of protective films 8 (electrical insulating films), the upper layer of protective film 8 is coated with medical adhesive and connected with the pulse electrical stimulation device 7, and the lower layer of protective film 8 is coated with medical adhesive and connected with the medicine cushion layer 10. And (3) sticking single-side glassine release paper 11 below the medicine cushion layer 10 to prepare the pulse electrical stimulation flexible electronic chitosan application.

The attending physician can adjust a suitable pulse electrical stimulation flexible electronic chitosan application, such as a circular application with the radius of 3cm, to emit pulse electrical stimulation of 30V,100pps and 100 mus according to the wound condition of the patient. According to the condition of the wound treated for the first time, a treatment scheme is established, and if the condition of the wound is poor, a doctor can give an order of stimulating for 1h every day. Patient tears from type paper 11 after going home, applies paste the flexible electron chitosan of pulse electrical stimulation and cover to the surface of a wound, and patient passes through wireless communication device 5 with the electrical stimulation command through the electrical stimulation parameter module at cell phone terminal, reachs early warning device 9, and early warning device 9 is behind 3 sound early warnings, and the electrical stimulation command conveys the flexible electron chitosan of pulse electrical stimulation of patient and applies ointment or plaster, and the electro photoluminescence treatment begins. The current sensor monitors whether a current load exists or not in each treatment period, if no current load exists, the current load is fed back to a mobile phone terminal of a patient through the wireless communication device 5, the early warning device 9 is started, the early warning device 9 gives an early warning to the patient through 5 sounds, and electrical stimulation treatment is directly carried out; if the current load exists, the actual treatment information is fed back to the mobile phone terminals of the doctor and the patient through the wireless communication device 5, so that the patient is treated regularly and humanizedly.

EXAMPLE 3A remote-controlled flexible electronic chitosan patch for electrical stimulation by pulses (Square; electrical stimulation parameters: 40V,105pps, 80 μ s; 1% chitosan mixture, weight ratio of vaseline to paraffin oil 2: 1; doctor's control via mobile phone)

Adding 0.5g of high molecular weight chitosan (molecular weight of 20 ten thousand and 50 ten thousand half) and 0.5g of low molecular weight chitosan (molecular weight of 5 ten thousand and 10 ten thousand half) into 99g of 0.5% sorbic acid solution, stirring uniformly to obtain 1% chitosan mixed solution, sterilizing and filtering; and (3) immersing the medical gauze into the chitosan mixed solution for 12 hours, taking out the medical gauze, and drying the medical gauze for 6 hours in a freeze-drying machine to obtain the gauze containing the 3% chitosan mixed solution. Coating vaseline and paraffin oil mixture (weight ratio of vaseline to paraffin oil is 2: 1) on the lower part of the dried medical gauze, and sterilizing with high pressure steam to obtain medicinal pad layer 10.

The warning device 9 and the wireless network communication device are the same as those of the

embodiment

1 or 2.

The early warning device 9 and the wireless network communication device are provided with the pulse electrical stimulation device 7 above, the pulse electrical stimulation device 7 is made of flexible electronic materials and comprises a flexible substrate 7-1, a battery 7-2 (a flexible thin film battery), a pulse switch circuit 7-3 (consisting of a direct current-direct current boosting module 7-4, a pulse modulation module 7-5 and a circuit switch 7-6), a current sensor 7-7, a positive electrode 7-9 and a negative electrode 7-10, and the above structures except the flexible substrate 7-1 are connected through a lead 7-8. The electrodes of the pulsed electrical stimulation device 7 are silver electrodes comprising peripheral positive electrodes 7-9 and central negative electrodes 7-10, all passing through a protective film 8 (electrical insulating film) and a drug pad layer 10. The pulse electrical stimulation device 7 can send out pulse electrical stimulation of 40V,105pps and 80 mus.

The backing layer 6 is self-adhesive non-woven fabric, and the connection mode of each layer of the flexible electronic chitosan application is electrically stimulated by pulse in the same way as in the

embodiment

1 or 2.

The attending physician adapts a suitable pulsed electrical stimulation flexible electronic chitosan patch, such as a square, 3 x 3cm, to deliver a pulsed electrical stimulation of 30V,100pps,100 mus, depending on the patient's wound condition (size, shape, infection, necrosis, etc.). According to the condition of the wound to be treated for the first time, a treatment scheme is established, and if the condition of the wound is normal, a doctor can give an order of stimulating for 1h every 2 days. Patient tears after getting home and from type paper 11, apply ointment or plaster the flexible electron chitosan of pulse electrical stimulation to cover to the surface of a wound, and the doctor passes through wireless communication device 5 with the electrical stimulation order through the electrical stimulation parameter module at cell phone terminal, reachs early warning device 9, and early warning device 9 is behind 3 sound early warnings, and the electrical stimulation order conveys to patient's pulse electrical stimulation flexible electron chitosan and applies ointment or plaster, and the electro photoluminescence treatment begins. The current sensor monitors whether a current load exists or not in each treatment period, if the current load does not exist when the expected treatment time is reached, the current load is fed back to a mobile phone terminal of a doctor through the wireless communication device 5, the early warning device 9 is started, the early warning device 9 gives an early warning to the patient through 5 sounds, and electrical stimulation treatment is directly carried out; if the current load exists, the actual treatment information is fed back to the mobile phone terminal of the doctor through the wireless communication device 5, so that the patient is treated regularly and in a humanized manner.

EXAMPLE 4A remote-controlled flexible electronic chitosan patch for electrical stimulation by pulses (rectangle; electrical stimulation parameters: 50V,100pps, 100. mu.s; 5% chitosan mixture; weight ratio of vaseline to paraffin oil 1: 1; controlled by the patient via mobile phone)

Adding 1g of high molecular weight chitosan (molecular weight is 20 ten thousand respectively) and 4g of low molecular weight chitosan (molecular weight is 5 thousand, 1 ten thousand is 1.5g respectively, molecular weight is 5 ten thousand and 1 ten thousand is 1g) into 95g of 1% sorbic acid solution, stirring uniformly to prepare 5% chitosan mixed solution, sterilizing and filtering; and (3) immersing the medical cotton pad into the chitosan mixed solution for 18 hours, taking out the cotton pad, and drying the cotton pad in a drying box at the temperature of 60-65 ℃ for 2 hours to obtain the cotton pad containing 5% of chitosan mixed solution. Coating vaseline and paraffin oil mixture (weight ratio of vaseline to paraffin oil is 1: 1) on the lower part of the dried medical sponge, and sterilizing with high pressure steam to obtain medicinal pad layer 10.

The early warning device 9 (an electronic alarm connected with a current sensor through a wire) and the Bluetooth communication device are connected with the pulse electrical stimulation device 7 and positioned between two layers of protective films 8(PET films).

The pulse electrical stimulation device 7 is arranged above the early warning device 9 and the Bluetooth communication device, the pulse electrical stimulation device 7 is made of flexible electronic materials and comprises a flexible substrate 7-1, a battery 7-2 (a button battery), a pulse switch circuit 7-3 (composed of a direct current-direct current boosting module 7-4, a pulse modulation module 7-5 and a circuit switch 7-6), a current sensor 7-7, a positive electrode 7-9 and a negative electrode 7-10, and the above structures except the flexible substrate 7-1 are connected through a lead 7-8. The electrodes of the pulse electrical stimulation device 7 are silver electrodes, and comprise positive electrodes 7-9 at the periphery and negative electrodes 7-10 at the center, and all the electrodes penetrate through a protective film 8(PET film) and a medicine pad layer 10. The pulse electrical stimulation device 7 can send out 50V,100pps and 100 mus pulse electrical stimulation.

The backing layer 6 is self-adhesive PU film, and the connection mode of the layers of the flexible electronic chitosan application is electrically stimulated by pulse in the same way as the

embodiment

1 or 2.

The attending physician adapts a suitable pulsed electrical stimulation flexible electronic chitosan patch, such as a rectangular patch, 5 x 10cm, to deliver pulsed electrical stimulation of 50V,100pps, 100 mus, depending on the wound condition (size, shape, infection, necrosis, etc.) of the patient. According to the condition of the wound treated for the first time, a treatment scheme is established, if the condition of the wound is poor, a doctor can give a prescription for electrically stimulating for 1h 2 times every day at an interval of 6 h. Patient tears from type paper 11 after going home, applies paste the flexible electron chitosan of pulse electrical stimulation and cover to the surface of a wound, and patient passes through wireless communication device 5 with the electrical stimulation command through the electrical stimulation parameter module at cell phone terminal, reachs early warning device 9, and early warning device 9 is behind 3 sound early warnings, and the electrical stimulation command conveys the flexible electron chitosan of pulse electrical stimulation of patient and applies ointment or plaster, and the electro photoluminescence treatment begins. The current sensor monitors whether a current load exists or not in each treatment period, if no current load exists, the current load is fed back to a mobile phone terminal of a patient through the wireless communication device 5, the early warning device 9 is started, the early warning device 9 gives an early warning to the patient through 5 sounds, and electrical stimulation treatment is directly carried out; if the current load exists, the actual treatment information is fed back to the mobile phone terminals of the doctor and the patient through the wireless communication device 5, so that the patient is treated regularly and humanizedly.

EXAMPLE 5A remotely controlled flexible electronic chitosan patch for electrical pulse stimulation (circular; electrical stimulation parameters: 60V,105pps, 80 μ s; 5% chitosan mixture, 1:2 by weight of petrolatum to paraffin oil; computer controlled by a doctor)

Adding 5g of a mixture of high molecular weight and low molecular weight chitosans (1 g of each of the molecular weights of 3 thousand, 5 ten thousand, 20 ten thousand, 30 ten thousand and 80 ten thousand) into 95g of 1% acetic acid solution, stirring uniformly to obtain 5% chitosan mixed solution, sterilizing and filtering; and (3) immersing the medical sponge into the chitosan mixed solution for 24 hours, taking out the sponge, and drying the sponge for 12 hours in a freeze-drying machine until no water drops, thus preparing the medical sponge containing 5% of chitosan mixed solution. Coating vaseline and paraffin oil mixture (weight ratio of vaseline to paraffin oil is 1:2, and sterilizing with high pressure steam) on the lower part of the dried medical sponge to obtain medicinal pad layer 10.

embodiment

1 or 2.

The early warning device 9 and the wireless network communication device are provided with the pulse electrical stimulation device 7 above, the pulse electrical stimulation device 7 is made of flexible electronic materials and comprises a flexible substrate 7-1, a battery 7-2 (a button battery), a pulse switch circuit 7-3 (consisting of a direct current-direct current boosting module 7-4, a pulse modulation module 7-5 and a circuit switch 7-6), a current sensor 7-7, a positive electrode 7-9 and a negative electrode 7-10, and the above structures except the flexible substrate 7-1 are connected through a lead 7-8. The electrodes of the pulse electro-stimulation device 7 are silver electrodes, and comprise peripheral positive electrodes 7-9 and central negative electrodes 7-10, all of which pass through a protective film 8 (electrical insulating film) and a drug pad layer 10, and all of which pass through the protective film 8(PET film) and the drug pad layer 10. The pulse electrical stimulation device 7 can send out 60V,105pps and 80 mus pulse electrical stimulation.

The backing layer 6 is self-adhesive PU film, and the connection mode of the layers of the flexible electronic chitosan application is electrically stimulated by pulse in the same way as the embodiment 1.

The attending physician adapts a suitable pulsed electrical stimulation flexible electronic chitosan patch, such as a circular patch, with a radius of 5cm, to deliver 50V,100pps, 100 μ s pulsed electrical stimulation, depending on the wound condition (size, shape, infection, necrosis, etc.) of the patient. According to the wound condition of the first diagnosis and treatment, a treatment scheme is established, and if the wound condition is better, a doctor can give an order of electrical stimulation for 1h every 2 days. Patient tears from type paper 11 after going home, applies ointment or plaster the flexible electron chitosan of pulse electrical stimulation and cover to the surface of a wound, and the doctor passes through wireless communication device 5 with the electrical stimulation order through the electrical stimulation parameter module at computer terminal, reachs early warning device 9, and early warning device 9 is behind 3 sound early warnings, and the electrical stimulation order conveys to patient's pulse electrical stimulation flexible electron chitosan and applies ointment or plaster, and the electro photoluminescence treatment begins. The current sensor monitors whether a current load exists or not in each treatment period, if no current load exists, the current load is fed back to a computer terminal of a doctor through the wireless communication device 5, the early warning device 9 is started, and the early warning device 9 gives an early warning to the patient through 5 sounds and directly performs electric stimulation treatment; if the current load exists, the actual treatment information is fed back to the computer terminal of the doctor through the wireless communication device 5, so that the patient is treated regularly and in a humanized manner.

EXAMPLE 6A remotely controlled flexible electronic chitosan patch for electrical pulse stimulation (Square; electrical stimulation parameters: 70V,100pps, 100. mu.s; 1% chitosan mixture, weight ratio of vaseline to paraffin oil 1: 1; patient controlled by mobile phone)

Adding 0.5g of high molecular weight chitosan (molecular weight of 20 ten thousand and 50 ten thousand half) and 0.5g of low molecular weight chitosan (molecular weight of 5 ten thousand and 10 ten thousand half) into 99g of 0.5% citric acid solution, stirring uniformly to obtain 1% chitosan mixed solution, sterilizing and filtering; and (3) soaking the absorbent cotton into the chitosan mixed solution for 24 hours, taking out the absorbent cotton, and drying the absorbent cotton in a freeze-drying machine for 12 hours to obtain the absorbent cotton containing 1% of chitosan mixed solution. Coating vaseline and paraffin oil mixture (weight ratio of vaseline to paraffin oil is 1: 1) on the lower part of the dried absorbent cotton, and sterilizing with high pressure steam to obtain medicinal pad layer 10.

The early warning device 9 and the wireless network communication device are the same as those in

embodiment

1 or 4.

The early warning device 9 and the wireless network communication device are provided with the pulse electrical stimulation device 7 above, the pulse electrical stimulation device 7 is made of flexible electronic materials and comprises a flexible substrate 7-1, a battery 7-2 (a button battery), a pulse switch circuit 7-3 (consisting of a direct current-direct current boosting module 7-4, a pulse modulation module 7-5 and a circuit switch 7-6), a current sensor 7-7, a positive electrode 7-9 and a negative electrode 7-10, and the above structures except the flexible substrate 7-1 are connected through a lead 7-8. The electrodes of the pulse electrical stimulation device 7 are titanium electrodes, and comprise positive electrodes 7-9 at the periphery and negative electrodes 7-10 at the center, and all the electrodes penetrate through a protective film 8(PET film) and a medicine pad layer 10. The pulse electrical stimulation device 7 can send out 70V,100pps and 100 mus pulse electrical stimulation.

The back lining layer 6 is self-adhesive air-permeable polyurethane film, and the connection mode of each layer of the pulse electrical stimulation flexible electronic chitosan application is the same as that of the

embodiment

1 or 2.

The attending physician adapts a suitable pulsed electrical stimulation flexible electronic chitosan patch, such as a square patch, 5 x 5cm, to deliver a pulsed electrical stimulation of 70V,100pps,100 mus, depending on the wound condition (size, shape, infection, necrosis, etc.) of the patient. According to the condition of the wound treated for the first time, a treatment scheme is established, and if the condition of the wound is poor, a doctor can give an order of 1h of daily electrical stimulation. Patient tears from type paper 11 after going home, applies paste the flexible electron chitosan of pulse electrical stimulation and cover to the surface of a wound, and patient passes through wireless communication device 5 with the electrical stimulation command through the electrical stimulation parameter module at cell phone terminal, reachs early warning device 9, and early warning device 9 is behind 3 sound early warnings, and the electrical stimulation command conveys the flexible electron chitosan of pulse electrical stimulation of patient and applies ointment or plaster, and the electro photoluminescence treatment begins. The current sensor monitors whether a current load exists or not in each treatment period, if no current load exists, the current load is fed back to a computer terminal of a doctor through the wireless communication device 5, the early warning device 9 is started, and the early warning device 9 gives an early warning to the patient through 5 sounds and directly performs electric stimulation treatment; if the current load exists, the actual treatment information is fed back to the computer terminal of the doctor through the wireless communication device 5, so that the patient is treated regularly and in a humanized manner.

EXAMPLE 7A remote-controlled flexible electronic chitosan patch for electrical stimulation by pulses (rectangle; electrical stimulation parameters: 80V,90pps, 90 μ s; 5% chitosan mixture; weight ratio of vaseline to paraffin oil: 2: 1; doctor's control via mobile phone)

Adding 2.5g of high molecular weight chitosan (molecular weight of 20 ten thousand and 50 ten thousand and half) and 2.5g of low molecular weight chitosan (molecular weight of 5 ten thousand and 10 ten thousand and half) into 95g of 1% acetic acid solution, stirring uniformly to obtain 5% chitosan mixed solution, sterilizing and filtering; and uniformly spraying the mixed solution on medical gauze, taking out the medical gauze, and drying the medical gauze in an oven at the temperature of 60-65 ℃ for 12 hours to prepare the washing cotton containing 5% of chitosan mixed solution. Coating vaseline and paraffin oil mixture (weight ratio of vaseline to paraffin oil is 2: 1) on the lower part of the dried absorbent cotton, and sterilizing with high pressure steam to obtain medicinal pad layer 10.

The warning device 9 and the Bluetooth communication device are the same as those in

embodiment

1 or 4.

The pulse electrical stimulation device 7 is arranged above the early warning device 9 and the Bluetooth communication device, the pulse electrical stimulation device 7 is made of flexible electronic materials and comprises a flexible substrate 7-1, a battery 7-2 (a button battery), a pulse switch circuit 7-3 (composed of a direct current-direct current boosting module 7-4, a pulse modulation module 7-5 and a circuit switch 7-6), a current sensor 7-7, a positive electrode 7-9 and a negative electrode 7-10, and the above structures except the flexible substrate 7-1 are connected through a lead 7-8. The electrodes of the pulse electro-stimulation device 7 are gold electrodes, comprising peripheral positive electrodes 7-9 and central negative electrodes 7-10, all passing through a protective film 8(PET film) and a drug pad layer 10. The pulse electrical stimulation device 7 can send out 80V,90pps and 90 mus pulse electrical stimulation.

embodiment

1 or 2.

The attending physician adapts a suitable pulsed electrical stimulation flexible electronic chitosan patch, such as a rectangular 10 x 10cm patch, to deliver 80V,90pps, 90 μ s pulsed electrical stimulation, depending on the wound condition (size, shape, infection, necrosis, etc.) of the patient. According to the condition of the wound treated for the first time, a treatment scheme is established, if the condition of the wound is poor, a doctor can give a prescription for electrically stimulating for 1h 2 times every day at an interval of 6 h. Patient tears after getting home and from type paper 11, apply ointment or plaster the flexible electron chitosan of pulse electrical stimulation to cover to the surface of a wound, and the doctor passes through wireless communication device 5 with the electrical stimulation order through the electrical stimulation parameter module at cell phone terminal, reachs early warning device 9, and early warning device 9 is behind 3 sound early warnings, and the electrical stimulation order conveys to patient's pulse electrical stimulation flexible electron chitosan and applies ointment or plaster, and the electro photoluminescence treatment begins. The current sensor monitors whether a current load exists or not in each treatment period, if no current load exists, the current load is fed back to a mobile phone terminal of a doctor through the wireless communication device 5, the early warning device 9 is started, and the early warning device 9 gives an early warning to the patient through 5 sounds and directly performs electric stimulation treatment; if the current load exists, the actual treatment information is fed back to the mobile phone terminal of the doctor through the wireless communication device 5, so that the patient is treated regularly and in a humanized manner.

EXAMPLE 8A remotely controlled flexible electronic chitosan patch for electrical pulse stimulation (circular; electrical stimulation parameters: 80V,90pps, 90 μ s; 5% chitosan mixture; weight ratio of vaseline to paraffin oil 1: 2; doctor controlled by computer)

Adding 5g of a mixture of high molecular weight chitosan and low molecular weight chitosan (1 g of each of the molecular weights of 3 thousand, 5 ten thousand, 20 ten thousand, 30 ten thousand and 80 ten thousand) into 95g of 0.5% citric acid solution, stirring uniformly to obtain a 5% chitosan mixed solution, sterilizing and filtering; soaking the medical cotton spunlace cloth in the chitosan mixed solution for 24 hours, taking out the medical cotton spunlace cloth, and drying the medical cotton spunlace cloth for 12 hours in a freeze-drying machine until no water drops, thus obtaining the medical cotton spunlace cloth containing 5% of chitosan mixed solution. Coating vaseline and paraffin oil mixture (weight ratio of vaseline to paraffin oil is 1: 2) on the lower part of the dried medical cotton spunlace fabric, and sterilizing with high pressure steam to obtain medicinal pad layer 10.

The early warning device 9 and the wireless communication device 5 are the same as those in

embodiment

1 or 2.

The wireless Bluetooth communication device is characterized in that a pulse electrical stimulation device 7 is arranged above the early warning device, the pulse electrical stimulation device 7 is made of flexible electronic materials and comprises a flexible substrate 7-1, a battery 7-2 (a flexible thin film battery), a pulse switch circuit 7-3 (composed of a direct current-direct current boosting module 7-4, a pulse modulation module 7-5 and a circuit switch 7-6), a current sensor 7-7, a positive electrode 7-9 and a negative electrode 7-10, and the above structures except the flexible substrate 7-1 are connected through a lead 7-8. The electrodes of the pulse electro-stimulation device 7 are titanium electrodes, and comprise peripheral positive electrodes 7-9 and central negative electrodes 7-10, all of which pass through a protective film 8 (electrical insulating film) and a drug pad layer 10. The pulse electrical stimulation device 7 can send out 100V,100pps and 100 mus pulse electrical stimulation.

embodiment

1 or 2.

The attending physician can adjust a suitable flexible electronic chitosan application for pulse electrical stimulation, such as a round application with a radius of 10cm, to give 100V,100, 100 pulse electrical stimulation according to the wound condition (size, shape, infection, necrosis, etc.) of the patient. According to the wound condition of the first diagnosis and treatment, a treatment scheme is established, and if the wound condition is more, a doctor can give an order of 1h of daily electrical stimulation. Patient tears from type paper 11 after going home, applies ointment or plaster the flexible electron chitosan of pulse electrical stimulation and cover to the surface of a wound, and the doctor passes through wireless communication device 5 with the electrical stimulation order through the electrical stimulation parameter module at computer terminal, reachs early warning device 9, and early warning device 9 is behind 3 sound early warnings, and the electrical stimulation order conveys to patient's pulse electrical stimulation flexible electron chitosan and applies ointment or plaster, and the electro photoluminescence treatment begins. The current sensor monitors whether a current load exists or not in each treatment period, if no current load exists, the current load is fed back to a computer terminal of a doctor through the wireless communication device 5, the early warning device 9 is started, and the early warning device 9 gives an early warning to the patient through 5 sounds and directly performs electric stimulation treatment; if the current load exists, the actual treatment information is fed back to the computer terminal of the doctor through the wireless communication device 5, so that the patient is treated regularly and in a humanized manner.

EXAMPLE 9 Experimental study on the promotion of wound healing in diabetic rats by a single-phase high-voltage pulse electrical stimulation flexible electronic chitosan application (electrical parameters: 40V,100pps, 100 μ s)

Normal SD rats were fed with a high-fat and high-sugar diet for one month and then injected with STZ (35mg/kg) to measure blood glucose. Random blood glucose>Taking 12 type II diabetes rats after successful model building, inducing and maintaining with gas anesthetic (isoflurane), preparing skin, and making 2 × 2cm of whole skin layer (including flesh membrane) at 2cm around the left and right parts of spinal column²Square wound surface of (2). The parameters of the pulse electrical stimulation device applied by the pulse electrical stimulation chitosan are set to 40V,100pps and 100 mus, and then the pulse electrical stimulation device is placed on the wound surface of a diabetic rat. The left wound was the control group and the right wound was the experimental group. Every 2 days for 1 hour. Photographs were taken at 3 rd, 7 th, 14 th and 21 st after surgery to record wound healing. The area of the wound surface was measured by Image J software, and statistical analysis of data was performed by GraphPad Prism software, data are expressed as SD + -SEM, p<A difference of 0.05 is statistically significant.

Fig. 5 is a comparison graph of wound healing of the single-phase high-voltage pulse electrostimulation chitosan patch applied to the wound of diabetic rats in example 9, which is photographs of the wound on

days

3, 7, 14 and 21. The left wound was the control group and the right wound was the experimental group. The results of the study showed that the wound surface covered with the single-phase high-voltage pulse electrostimulation chitosan patch healed faster than the control group, as shown in fig. 6. A is a statistical chart of the wound area of a single-phase high-voltage group; b is a statistical chart of the wound healing rate of the monophasic high-voltage group. The results show that the application of the monophasic high-voltage pulse electrical stimulation chitosan (electrical parameters: 40V,100pps, 100 mus) can accelerate the healing of the wound of the diabetic rat.

EXAMPLE 106 Experimental study of groups of Electrical stimulation with different electrical parameters on chitosan application (surface electrode) to promote wound healing in diabetic rats

Normal SD rats were fed with a high-fat and high-sugar diet for one month and then injected with STZ (35mg/kg) to measure blood glucose. The random blood sugar of more than 16.7mmol/L is the standard for the model building success of the type II diabetic rats. 72 type II diabetic rats successfully molded were randomly divided into 6 groups (12 rats per group), and the electrical parameters were grouped into a single-phase high-voltage group (40V, 100. mu.s, 100pps), a single-phase low-voltage group (4V, 100. mu.s, 100pps), a single high-voltage + direct current (40V, 100. mu.s, 100 pps; 4V), a direct current group (4V), a two-phase high-voltage group (. + -. 40V, 100. mu.s, 100pps), and a two-phase low-voltage group (. + -. 4V, 100. mu.s, 100 pps).

Maintaining under induction of gas anesthetic (isoflurane), preparing skin, and making 2 × 2cm of full skin layer (including periosteum) at 2cm around the left and right parts of spinal column²Square wound surface of (2). Then an electrode (surface electrode) applied with chitosan by pulse electrical stimulation is placed on the wound surface of a diabetic rat, a negative electrode is placed at the center of the wound surface, and a positive electrode is placed at a normal skin position 1cm away from the edge of the wound. The left wound was the control group and the right wound was the experimental group. Every 2 days for 1 hour. Photographs were taken at 3 rd, 7 th, 14 th and 21 st after surgery to record wound healing. The area of the wound surface was measured by Image J software, and statistical analysis of data was performed by GraphPad Prism software, data are expressed as SD + -SEM, p<A difference of 0.05 is statistically significant.

Fig. 7 is a photograph of wound surface of diabetic rat on

days

3, 7, 14 and 21 after applying 6 groups of pulse electrical stimulation chitosan patch with different electrical parameters in this example 10, wherein the left side wound surface is a control group, and the right side wound surface is an electrical stimulation experimental group.

Referring to fig. 8 and 9, wherein fig. 8A-D are statistical plots of the healing rate for 6 sets of different electrical parameters of pulsed electrical stimulation of

chitosan application days

3, 7, 14, 21; fig. 9A-D are statistical graphs of the healing-promoting rates for 6 groups of different electrical parameters of the pulsed electrical stimulation chitosan application on

days

3, 7, 14, and 21 (experimental group-control wound healing rate); fig. 9E is a line graph of a statistical graph of the healing-promoting rates (experimental group healing rate-control wound healing rate) for 6 groups of pulsed electrical stimulation chitosan patches with different electrical parameters on

days

3, 7, 14, and 21; FIG. 9F is a comparison of the number of healing days for 6 sets of pulsed electrical stimulation of chitosan applications with different electrical parameters; experimental results show that the single-phase high-voltage pulse electrical stimulation group and the single-phase high-voltage pulse electrical stimulation and direct current electrical group can promote healing of the wound surface of type ii diabetes (fig. 9A-D), and comparison of data of 6 groups of different electrical parameters shows that the unidirectional high-voltage pulse electrical stimulation group (HVMPC) can promote healing of the wound surface of diabetes most effectively, and is the optimal electrical stimulation parameter (fig. 9). And (4) prompting by a result: unidirectional high voltage pulsed electrical stimulation (HVMPC) may be the optimal electrical stimulation.

EXAMPLE 116 Experimental study of groups of different electrical parameters of pulsed electrostimulation chitosan applications (inserted minimally invasive electrodes) to promote healing of diabetic rat wounds

Normal SD rats were fed with a high-fat and high-sugar diet for one month and then injected with STZ (35mg/kg) to measure blood glucose. The random blood sugar of more than 16.7mmol/L is the standard for the model building success of the type II diabetic rats. 72 type II diabetic rats successfully molded are randomly divided into 6 groups (12 rats in each group), namely a monophasic high-voltage pulse electrical stimulation group (40v,100 pps,100 mus), a monophasic low-voltage pulse electrical stimulation group (4v,100 pps,100 mus), a biphasic high-voltage pulse electrical stimulation group (40v,100 pps,100 mus), a biphasic low-voltage pulse electrical stimulation group (4v,100 pps,100 mus), a direct-current electrical stimulation group (4v) and a monophasic high-voltage pulse electrical stimulation composite direct-current electrical stimulation group (40v,100 pps,100 mus; 4 v).

Maintaining under induction of gas anesthetic (isoflurane), preparing skin, and making 2 × 2cm of full skin layer (including periosteum) at 2cm around the left and right parts of spinal column²Square wound surface of (2). Then the pulsed electrical stimulation is chitosanThe electrodes (inserted minimally invasive electrodes) applied with sugar are placed on the wound surface of a diabetic rat, the negative electrode is placed in the center of the wound surface, and the positive electrode is placed on the normal skin 1cm away from the edge of the wound. The left wound was the control group and the right wound was the experimental group. Every 2 days for 1 hour. Photographs were taken at 3 rd, 7 th, 14 th and 21 st after surgery to record wound healing. The area of the wound surface was measured by Image J software, and statistical analysis of data was performed by GraphPad Prism software, data are expressed as SD + -SEM, p<A difference of 0.05 is statistically significant.

Referring to fig. 10, it is a photograph of wound surface of diabetic rat on

days

3, 7, 14, and 21 with 6 groups of electrical stimulation chitosan application (inserted minimally invasive electrodes) with different electrical parameters applied in this example 11, the left side wound surface is a control group, and the right side wound surface is an electrical stimulation experimental group.

Referring to fig. 11 and 12, wherein fig. 11A-D are statistical plots of the healing rates of 6 sets of pulsed electrical stimulation chitosan patches (inserted minimally invasive electrodes) of different electrical parameters applied to diabetic rat wounds on

days

3, 7, 14, and 21; fig. 12A-D are statistical plots of the healing-promoting rates for 6 groups of different electrical parameters of pulsed electrical stimulation chitosan applications (inserted minimally invasive electrodes) on

days

3, 7, 14, and 21 (experimental group healing-control wound healing); fig. 12E is a line graph of a statistical graph of the healing-promoting rates (experimental group healing rate-control wound healing rate) for 6 groups of pulsed electrical stimulation chitosan patches (inserted minimally invasive electrodes) of different electrical parameters on

days

3, 7, 14, and 21; figure 12F is a comparison of the number of healing days for 6 sets of pulsed electrical stimulation chitosan applications (inserted minimally invasive electrodes) of different electrical parameters.

The impedance of the rat wound surface epidermis is the maximum, so the positive electrode surface electrode is improved into an insertion type minimally invasive electrode. However, experimental results found that monophasic high voltage pulsed electricity was the best electrical stimulation parameter in 6 groups as was the preferred result of experiments with surface electrodes (fig. 10-12). The healing rate of the right side electrical stimulation treatment wound surface of the unidirectional high voltage group alone was faster than that of the left side control wound surface, and the right side electrical stimulation wound surface of the direct current group was even slower than that of the left side control group (fig. 11-12). Compared with the prior surface electrode, the minimally invasive electrode has no more obvious effect of promoting wound healing, the electric current is too large due to too small resistance between the two electrodes to generate heat effect, so that the skin is burnt, 4 groups of electric stimulation groups have electric burn phenomena, and 2 groups of injuries with direct current are more obvious (HVMPC and HVMPC + DC groups, figure 10). And (4) prompting by a result: the electrode for electrical stimulation is preferably a surface electrode.

Example 12 in vitro experiment of Electrical stimulation to promote migration of HUVEC cells

HUVEC cultured in high-sugar and high-fat (25mmol/L D-glucose and 0.4mM sodium palmitate) environment simulates in-vivo environment of diabetes, and whether electrical stimulation can promote migration of HUVEC cells is studied by using a scratch test and a Transwell migration test. Cell scratch assay is a useful assay to study cell migration in vitro. The principle is that a blank cell-free zone is artificially made in a single layer of plated cells, and then cells at the edge of the cell-free zone are observed; the cells at these edges will begin to migrate and eventually cover the entire cell-free area and come back into contact with each other. The Tanswell experiment principle is that a Transwell chamber is placed in a culture plate, an upper chamber is called in the chamber, a lower chamber is called in the culture plate, an upper layer culture solution is contained in the upper chamber, a lower layer culture solution is contained in the lower chamber, and the upper layer culture solution and the lower layer culture solution are separated by a polycarbonate membrane. When the cells are planted in the upper chamber, due to permeability of the polycarbonate membrane, the components in the lower layer culture solution can affect the cells in the upper chamber, the cells can run to the lower chamber with high nutrient content, the migration capacity of the cells can be reflected by counting the amount of the cells entering the lower chamber, and therefore the influence of the components in the lower layer culture solution on the growth, movement and the like of the cells can be researched.

FIG. 13 shows the results of the in vitro experiment of this example 12 in which electrical stimulation promoted migration of HUVEC cells. The experiments were grouped into control groups (control, no electrical stimulation applied), and 4 electrical stimulation experimental groups: 4V 5min, 4V 10min, 40V, 5min and 40V,10 min. FIG. 13A is a graph of gentian violet staining after 12h of a Transwell experiment; FIG. 13B is a graph showing migration after 12h, 24h and 36h of the cell scratch test; fig. 13C is a mobility statistic of the cell scratch experiment (15B). The experimental result shows that the electrical stimulation can promote the migration of HUVEC, thereby being beneficial to the migration of repairing cells to the wound surface and participating in the healing process.

Example 13 Electrical stimulation of HUVEC cells to promote secretion of various growth factors

HUVECs cultured in a high-sugar, high-lipid (25mmol/L D-glucose, 0.4mM sodium palmitate) environment mimic the diabetic in vivo environment. HUVEC cells secreting various growth factors were detected by Western immunoblotting (WB) and enzyme-linked immunosorbent assay (ELISA). The WB assay is a method for detecting a certain protein in a complex sample based on specific binding of antigen-antibody. The method is a new immune biochemical technology developed on the basis of gel electrophoresis and solid-phase immunoassay, has the high resolution of SDS-PAGE and the high specificity and sensitivity of solid-phase immunoassay, and is a conventional technology for protein analysis. Immunoblotting is commonly used to identify certain proteins and enables qualitative and semi-quantitative analysis of proteins. In the ELISA test, a known antigen or antibody is adsorbed on the surface of a solid phase carrier, and an enzyme-labeled antigen-antibody reaction is allowed to proceed on the surface of the solid phase. The technology can be used for detecting macromolecular antigens, specific antibodies and the like, and has the advantages of rapidness, sensitivity, simplicity, convenience, easy standardization of carriers and the like.

FIG. 14 shows the results of experiments performed in this example 13 in which electrical stimulation promoted secretion of various factors by HUVEC cells. Groups were divided into control (no electrical stimulation applied), and 4 electrical stimulation experimental groups: 4V 5min, 4V 10min, 40V, 5min and 40V,10 min. FIG. 14A is a Western blot chart; FIG. 14B is a statistic of relative protein expression; FIG. 14C is Elisa results statistics of VEGF. The results show that compared with a control group, the experimental group can promote the expression of VEGF, FGF-2, TGF-beta 1, Akt, p-Akt and p-ERK1/2, thereby accelerating the healing of the wound surface.

Claims

1. A remote-control pulse electrical stimulation flexible electronic chitosan application is characterized by consisting of a terminal (1) and a pulse electrical stimulation flexible electronic chitosan application (2), the terminal (1) comprises an application program (3) for electrically stimulating the flexible electronic chitosan application by pulse and a terminal communication device (4), the pulse electrical stimulation flexible electronic chitosan application (2) comprises a wireless communication device (5), a back lining layer (6), a pulse electrical stimulation device (7), a protective film (8), an early warning device (9), a medicine cushion layer (10) and release paper (11), and sequentially comprises a back lining layer (6), a pulse electrical stimulation device (7), a protective film (8), a drug cushion layer (10) and release paper (11) from top to bottom, wherein the wireless communication device (5) and the early warning device (9) are simultaneously positioned between the two layers of protective films (8).

2. The remotely controlled pulsed electrical stimulation flexible electronic chitosan applicator of claim 1, wherein the pulsed electrical stimulation flexible electronic chitosan applicator application (3) comprises a patient information module (3-1), an electrical stimulation parameter module (3-2) and a communication module (3-3).

3. The remote-control pulse electrical stimulation flexible electronic chitosan application as claimed in claim 1, wherein the pulse electrical stimulation device (7) is located in the center of the backing layer (6), the protective film (8) has two layers, the upper protective film (8) is coated with medical adhesive and connected with the pulse electrical stimulation device (7), the lower protective film (8) is coated with medical adhesive and connected with the drug cushion layer (10), the wireless communication device (5) and the early warning device (9) are arranged between the two protective films (8), the lower surface of the drug cushion layer (10) is bonded with release paper (11), and the positive and negative electrodes in the pulse electrical stimulation device (7) penetrate through the two protective films (8) and the drug cushion layer (10).

4. A remote controlled pulsed electrical stimulation flexible electronic chitosan dressing according to claim 1 or 3, characterized in that the pulsed electrical stimulation device (7) comprises a flexible substrate (7-1), a battery (7-2), a pulsed switching circuit (7-3), a current sensor (7-7), a lead (7-8), a positive electrode (7-9) and a negative electrode (7-10), wherein the battery (7-2) is a button cell or a flexible thin film battery; the pulse switch circuit (7-3) consists of a direct current-direct current boosting module (7-4), a pulse modulation module (7-5) and a circuit switch (7-6); the sensors (7-7) are current sensors, wherein the positive electrodes (7-9) and the negative electrodes (7-10) of the electrodes penetrate through the two layers of protective films (8) and the drug cushion layer (10).

5. A remote controlled pulsed electro-stimulation flexible electronic chitosan applicator as claimed in claim 1, wherein said pre-warning means (9) is an electronic alarm connected to the sensors (7-7) by wires.

6. A remotely controlled pulsed electrical stimulation flexible electronic chitosan dressing as claimed in claim 1 or 3, wherein the backing layer (6) is glued to the pulsed electrical stimulation device (7), the drug pad layer (10), and the exposed outer peripheral portions of the release paper (11) to reinforce the gluing of the respective portions to the backing layer (6) and release paper (11) as a whole, said exposed peripheral portions being the portions of each layer which have not been glued to the other layers but have been coated with glue.

7. The remotely controlled pulse electrical stimulation flexible electronic chitosan application as claimed in claim 1 or 3, wherein the backing layer (6) is selected from self-adhesive air-permeable polyurethane film, PU film, non-woven fabric; the pulse electrical stimulation device (7) is made of flexible electronic materials; the protective film (8) is made of an insulating material and is a high-temperature-resistant polyester film or an electrical insulating film; the medical pad layer (10) is made of medical materials with adsorption capacity, and is made of absorbent cotton, medical gauze, medical sponge, medical cotton pad and medical cotton spunlace cloth; the terminal (1) is a mobile phone or a computer terminal, and the terminal communication device (4) and the wireless communication device (5) are Bluetooth communication devices or wireless network communication devices.

8. A remote controlled pulsed electrical stimulation flexible electronic chitosan dressing according to claim 1 or 3, characterized in that the pulsed electrical stimulation flexible electronic chitosan dressing (2) has a shape of square, circle, triangle, the shape and size of which are specifically determined according to the shape of the wound.

9. The remote-control flexible electronic chitosan application electrically stimulated by pulses as claimed in claim 7, wherein after the pulse electrical stimulation device (7) is manufactured, a waterproof glue for a circuit board is sprayed to prevent water from permeating to cause short circuit.