CN211484518U - Multispectral fluorescence imaging device for living animals - Google Patents

Abstract

The utility model discloses a multispectral fluorescence imaging device for live body animal, include: the system comprises an excitation light source module, a fluorescence imaging module, a master control computer, an imaging camera bellows module, a living animal storage module and an anesthesia and life support module; the living animal storage module is arranged inside the imaging camera bellows module and is used for storing living animals; the excitation light source module is connected with the imaging dark box module and is used for generating excitation light for irradiating living animals; the fluorescence imaging module is arranged on the imaging camera bellows module and is used for carrying out fluorescence imaging on the living animal; the anesthesia and life support module is used for providing anesthesia gas and air for maintaining living of the living animal. The utility model discloses can realize the multispectral fluorescence imaging to the live body animal, the utility model discloses have fine protection to the environment of depositing of live body toy, can guarantee the safety of waste gas, prevent that the metabolite of live body animal from polluting the external environment.

Description

Multispectral fluorescence imaging device for living animals

Technical Field

The utility model relates to a medical imaging technical field, in particular to a multispectral fluorescence imaging device for live body animal.

Background

In recent years, with the development of biology, small animal living body imaging instruments are widely used more and more, and are used as one of the most important instruments for living body animal experiments, the living body imaging instruments have the advantages of real-time imaging, no damage to animals, long-time observation and monitoring and the like, so that the normal physiological activity of experimental animals is ensured to the maximum extent, the interference and the damage of human factors are avoided, and the real pathological phenomenon is most approximate. In contrast, animal dissection and pathological section experiments firstly require the sacrifice of animals, and a series of mechanical cutting and chemical drug treatment are performed on organ tissues of the animals, so that tissue injury deformation and cell apoptosis necrosis are caused in the sampling and sample preparation processes, the real physiological state is damaged, false positive or false negative results are generated, and if not taken care of, misjudgment can be caused, and an erroneous experimental conclusion is obtained.

The animal living body imaging technology is characterized in that qualitative and quantitative researches on tissue, cell and even molecular level are carried out on biological processes in a living body state by applying an imaging method. Animal in vivo imaging techniques are mainly divided into five major categories, optical imaging, nuclear species imaging (PET/SPECT), Magnetic Resonance Imaging (MRI), Computed Tomography (CT), and ultrasound (ultrasounds). Among them, optical imaging and nuclear imaging are particularly suitable for studying molecular, metabolic and physiological events, called functional imaging; ultrasound and CT are suitable for anatomical imaging, known as structural imaging; MRI is intermediate between functional and structural imaging. Viruses can be genetically or chemically modified to carry specific optical molecular markers, and therefore optical imaging is the most suitable in vivo imaging technique for virology research based on the characteristics of the virus and the research purpose.

The in-vivo fluorescence imaging instrument is mainly used for qualitative and quantitative research on tissues, cells and even molecular levels in biological processes in a living body state, wherein the fluorescence imaging is particularly suitable for in-vivo imaging of viruses with specific optical molecular markers through gene modification or chemical modification. Currently, in vivo fluorescence imaging devices have a number of disadvantages, such as: the existing imaging equipment has huge and complex structure and high cost, the existing imaging equipment does not protect the storage environment of the living small animals, and metabolites of the small animals cannot be processed in time in the shooting process.

SUMMERY OF THE UTILITY MODEL

The technical problem to be solved by the present invention is to provide a multispectral fluorescence imaging device for living animals, which is not enough for the above prior art.

In order to solve the technical problem, the utility model discloses a technical scheme is: a multi-spectral fluorescence imaging apparatus for living animals, comprising: the system comprises an excitation light source module, a fluorescence imaging module, a master control computer, an imaging camera bellows module, a living animal storage module and an anesthesia and life support module;

the living animal storage module is arranged inside the imaging camera bellows module and is used for storing living animals; the excitation light source module is connected with the imaging dark box module and is used for generating excitation light for irradiating living animals; the fluorescence imaging module is arranged on the imaging camera bellows module and is used for carrying out fluorescence imaging on the living animal; the anesthesia and life support module is used for providing anesthesia gas and air for maintaining living of the living animal.

Preferably, the excitation light source module includes a light source, a coupling lens, a first optical filter and an optical fiber;

the first optical filter is arranged on a first optical filter wheel, the first optical filter wheel is arranged on a rotating shaft, and the excitation light source module further comprises a first motor for driving the rotating shaft to rotate; the optical fiber is arranged on the optical fiber sleeve, the tail end of the optical fiber is connected with a beam expander and extends into the imaging camera bellows module to irradiate the living animal.

Preferably, the fluorescence imaging module comprises a motorized zoom lens, a second optical filter and a camera, the motorized zoom lens extends into the imaging camera bellows module, and the camera is connected with the master control computer;

exciting light emitted by the light source sequentially passes through the coupling lens and the first optical filter and then enters the optical fiber, and then passes through the beam expanding lens at the tail end of the optical fiber and then irradiates on the living animal, and fluorescence generated after fluorescent substances in the living animal body are excited sequentially passes through the motorized zoom lens and the second optical filter and then enters the camera.

Preferably, the fluorescence imaging module further comprises a mounting housing disposed on the imaging camera bellows module, a second filter wheel disposed in the mounting housing, and a second motor disposed on the mounting housing and used for driving the second filter wheel to rotate, wherein the second filter is mounted on the second filter wheel; the camera is connected with a focus adjusting seat, and a differential adjusting head is arranged on the focus adjusting seat.

Preferably, the imaging camera bellows module comprises a box body, a sealing door arranged on the box body, a support frame arranged inside the box body, a lens support plate arranged inside the box body and an adjusting platform arranged at the bottom inside the box body.

Preferably, the adjusting platform can move in a horizontal plane on a bottom plate of the box body, and the motorized zoom lens is arranged on the lens supporting plate;

the sealing door is connected with the box body through a hinge, and a locking mechanism is arranged on the sealing door.

Preferably, the living animal storage module is arranged on the adjusting platform and comprises a sealed cabin body for storing living animals, a negative pressure maintaining module for maintaining a negative pressure environment in the sealed cabin body, and an air filtering module for filtering air entering and exiting the sealed cabin body.

Preferably, a window sheet is arranged on the top of the sealed cabin body, and both the excitation light generated by the excitation light source module and the fluorescence generated after the fluorescent substance in the living animal body is excited can penetrate through the window sheet.

Preferably, a plurality of positioning blocks are arranged at the bottom of the sealing bin body, and a plurality of positioning mounting plates corresponding to the positioning blocks are arranged on the adjusting platform.

Preferably, the positioning mounting plate is provided with a positioning hole, the positioning hole comprises a conical guide hole and a cylindrical positioning hole which are sequentially formed from the surface of the positioning mounting plate downwards, and the side part of the positioning mounting plate is provided with a locking hole which vertically penetrates through the cylindrical positioning hole; a locking column is movably inserted into the locking hole, a chuck is arranged at the outer end of the locking column, a pressure spring is arranged between the chuck and the side wall of the positioning mounting plate, a spherical surface is arranged at the inner end of the locking column, and the inner end of the locking column extends into the cylindrical positioning hole;

the bottom of the positioning block is fixedly connected with a positioning column which is inserted into the positioning hole, an insertion hole is formed in the positioning column in the direction vertical to the axial direction of the positioning column, and an inclined guide surface is arranged on the side surface of the bottom of the positioning column, facing the inner end of the locking column;

after the positioning column is inserted into the positioning hole, the inner end of the locking column is inserted into the insertion hole of the positioning column in a matched mode so as to lock the positioning column.

The utility model has the advantages that: the utility model can realize multispectral fluorescence imaging of the living body animal, has good protection to the storage environment of the living body small animal, can ensure the safety of waste gas, and prevents the metabolite of the living body animal from polluting the external environment; the living animal storage module of the utility model can be rapidly installed and positioned in the imaging camera bellows module, thereby facilitating imaging; the utility model discloses each subassembly is arranged compactly rationally, simple structure, stability, and the cost is cheap, can satisfy live body animal fluorescence imaging's demand, has fine application prospect.

Drawings

Fig. 1 is a schematic structural view of a multispectral fluorescence imaging device for living animals according to the present invention;

FIG. 2 is a schematic view of the internal structure of the imaging camera bellows module of the present invention;

FIG. 3 is a schematic view of the external structure of the imaging camera bellows module of the present invention;

fig. 4 is a schematic structural diagram of components inside an excitation light source module according to the present invention;

FIG. 5 is a schematic structural diagram of a fluorescence imaging module according to the present invention;

fig. 6 is a light path diagram of the multispectral fluorescence imaging device for living animals according to the present invention;

fig. 7 is a bottom view of the adjustable platform of the present invention;

fig. 8 is a schematic structural view of the positioning mounting plate in the top view direction according to the present invention;

fig. 9 is a schematic structural view of the positioning block according to the present invention in a side view direction;

fig. 10 is a schematic structural view of another side view of the positioning block of the present invention;

fig. 11 is a structural schematic view of the sealed cabin body of the present invention in the bottom view direction;

fig. 12 is a cross-sectional view of the positioning mounting plate of the present invention;

fig. 13 is a schematic structural diagram of the positioning block and the positioning mounting plate of the present invention.

Description of reference numerals:

1-excitation light source module; 10-a light source; 11-a coupling lens; 12 — a first filter; 13-an optical fiber; 14 — a first filter wheel; 15-a rotating shaft; 16 — a first motor; 17-optical fiber sleeve; 18-optical fiber head fixing seat; 19-a beam expander;

2-a fluorescence imaging module; 20-a motorized zoom lens; 21-a second filter; 22-a camera; 23-mounting the housing; 24-a second motor; 25-focus adjusting seat; 26-differential adjustment head;

3, a master control computer;

4-imaging camera bellows module; 40, a box body; 41-sealing door; 42-a support frame; 43-lens support plate; 44-adjusting the platform; 45-folding; 46-a locking mechanism;

5-a live animal storage module; 50, sealing the bin body; 51-window piece;

6, positioning blocks; 60-a positioning column; 61-a jack; 62 — an inclined guide surface;

7, positioning a mounting plate; 70-positioning holes; 71-a tapered pilot hole; 72-cylindrical positioning holes; 73-locking holes; 74-locking post; 75-a chuck; 76-pressure spring; 77-spherical surface;

8-anesthesia and life support module;

9-live animals.

Detailed Description

The present invention is further described in detail below with reference to examples so that those skilled in the art can implement the invention with reference to the description.

It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.

As shown in fig. 1 to 3, a multispectral fluorescence imaging apparatus for a living animal according to the present embodiment includes: the system comprises an excitation light source module 1, a fluorescence imaging module 2, a master control computer 3, an imaging dark box module 4, a living animal storage module 5 and an anesthesia and life support module;

the living animal storage module 5 is arranged inside the imaging camera bellows module 4 and is used for storing living animals; the excitation light source module 1 is connected with the imaging dark box module 4 and is used for generating excitation light for irradiating living animals; the fluorescence imaging module 2 is arranged on the imaging camera bellows module 4 and is used for carrying out fluorescence imaging on the living animal; the anesthesia and life support module is used to provide anesthetic gases and air to maintain the living animal.

The excitation light source module 1 comprises a light source 10, a coupling lens 11, a first optical filter 12 and an optical fiber 13;

the first optical filter 12 is installed on a first optical filter wheel 14, the first optical filter wheel 14 is arranged on a rotating shaft 15, and the excitation light source module 1 further comprises a first motor 16 for driving the rotating shaft 15 to rotate; the optical fiber 13 is installed on the optical fiber sleeve 17, and the tail end of the optical fiber 13 is connected with a beam expander 19 and extends into the imaging camera bellows module 4 to irradiate the living animal. The light source 10, the coupling lens 11, the first filter 12, the first filter wheel 14 and the first motor 16 are all arranged inside the box 40 of the light source 10 module, and the excitation light is transmitted out through the optical fiber 13 and enters the imaging dark box module 4. In a preferred embodiment, the light source 10 is a xenon lamp light source 10, the excitation light emitted by the light source 10 is reflected by a concave mirror, and then passes through a coupling lens 11 to couple the light beam into an optical fiber 13, a first filter wheel 14 is disposed at the front end of the optical fiber 13, in this embodiment, the first filter wheel 14 is an 8-bit filter wheel on which 8 first filters 12 can be disposed, the excitation light can pass through the first filters 12 and then enter the optical fiber 13, and the wavelength of the excitation light can be adjusted by replacing different first filters 12. In this embodiment, the light is a four-in-one optical fiber 13, the end of the four optical fibers 13 is connected with a beam expander 19, and optical fiber head fixing seats 18 are arranged at four corners inside the imaging camera bellows module 4, and are used for installing and fixing the end of the four optical fibers 13, and the optical fiber head fixing seats 18 can also adjust the positions of the ends of the four optical fibers 13. The excitation light irradiates the living animal through four optical fibers 13 to meet the requirement of fluorescence imaging.

The fluorescence imaging module 2 comprises a motorized zoom lens 20, a second optical filter 21 and a camera 22, wherein the second optical filter 21 is positioned between the motorized zoom lens 20 and the camera 22, the motorized zoom lens 20 extends into the imaging camera bellows module 4, and the camera 22 is connected with the main control computer 3;

referring to fig. 6, excitation light emitted from a light source 10 sequentially passes through a coupling lens 11 and a first filter 12, enters an optical fiber 13, passes through a beam expander 19 at the end of the optical fiber 13, and is irradiated onto a living animal, and fluorescence generated after a fluorescent substance in the living animal is excited sequentially passes through a motorized zoom lens 20 and a second filter 21, and then enters a camera 22.

The fluorescence imaging module 2 further comprises a mounting case 23 arranged on the imaging camera bellows module 4, a second filter 21 wheel arranged in the mounting case 23, and a second motor 24 arranged on the mounting case 23 and used for driving the second filter 21 wheel to rotate, wherein the second filter 21 is arranged on the second filter 21 wheel, and the second filter 21 wheel is arranged inside the mounting case 23 and is not shown in the figure. The camera 22 is connected with a focus adjusting base 25, and the focus adjusting base 25 is provided with a differential adjusting head 26. In a preferred embodiment, the working distance of the motorized zoom lens 20 can satisfy 220-380 mm. 8 hole sites are arranged on the wheel of the second optical filter 21, 8 second optical filters 21 can be arranged, and a Hall sensor is further arranged in the installation shell 23, so that the position of the wheel of the second optical filter 21 can be detected. The differential adjustment head 26 is disposed between the focus adjustment base 25 and the mounting housing 23, and the distance between the camera 22 and the motorized zoom lens 20 can be adjusted by the differential adjustment head 26 to adjust the focus, so that the light passing through the second optical filter 21 is focused on the focal plane of the camera 22. The motorized zoom lens 20 and the second filter 21 wheel are connected with a circuit board, the circuit board is connected and communicated with the main control computer 3, and the motorized zoom lens 20 and the second filter 21 wheel can be controlled through the main control computer 3. The camera 22 is connected with the master control computer 3, and the master control computer 3 can process the image collected by the camera 22.

The camera bellows module 4 includes a housing 40, a sealing door 41 disposed on the housing 40, a support frame 42 disposed inside the housing 40, a lens support plate 43 disposed inside the housing 40, and an adjustment platform 44 disposed at the bottom inside the housing 40. In order to ensure the performance of the imaging camera bellows module 4, the material of the imaging camera bellows module 4 can be blackened, and the stability of the light path in the detection process is ensured. The support brackets 42 provide strength on the one hand and provide secondary protection against possible gaps at the corner joints of the cabinet 40 on the other hand. The sealing door 41 is provided with a sealing rubber ring, and the inner sealing rubber ring is compressed tightly when the door plate is closed, so that the door plate has good shading performance.

The adjustment platform 44 can move in the horizontal plane on the bottom plate of the box 40, and can be realized by arranging a slide block and a slide rail between the adjustment platform 44 and the bottom plate of the box 40. After the living animal storage module 5 is placed on the adjusting platform 44, the horizontal position of the living animal storage module 5 is adjusted by the adjusting platform 44, and then the living animal storage module is fixed (which can be realized by arranging the locking mechanism 46 on the sliding block), so that imaging is facilitated.

The motorized zoom lens 20 is disposed on the lens support plate 43. The sealing door 41 is connected to the box 40 through a hinge 45, and a locking mechanism 46 is provided on the sealing door 41.

The living animal storage module 5 is disposed on the adjusting platform 44, and includes a sealed cabin 50 for storing living animals, a negative pressure maintaining module (not shown in the figure) for maintaining a negative pressure environment in the sealed cabin 50, and an air filtering module (not shown in the figure) for filtering air entering and exiting the sealed cabin 50. The sealed cabin 50 is provided with an anesthesia air port, an air inlet and an air outlet, the negative pressure maintaining module can be a centrifugal fan and a matched pipeline, air in the sealed cabin 50 is continuously pumped out through the air outlet, negative pressure is kept in the sealed cabin 50, and outside air enters the sealed cabin 50 through the air port. The air filtration module is a high-efficiency filter, and is used for filtering air inlet and air outlet of the sealed cabin body 50, so that the safety of waste gas is ensured, and the condition that the external environment is polluted by metabolites of living animals is prevented. The anesthetic gas supplied through the anesthetic gas port can maintain the silence of the living animal during photographing.

The top of the sealed cabin 50 is provided with a window sheet 51, and both the excitation light generated by the excitation light source module 1 and the fluorescence generated by the fluorescent substance in the living animal body after being excited can penetrate through the window sheet 51. The light ray end of the excitation light source module 1 is positioned above the window sheet 51, and the light ray end is connected with the beam expander 19, so that the excitation light beam can cover the living animal. The motorized zoom lens 20 is also located above the window sheet 51, and is used to photograph a living animal.

In one embodiment, the positioning mounting plate 7 is provided with a positioning hole 70, the positioning hole 70 comprises a conical guide hole 71 and a cylindrical positioning hole 72 which are sequentially formed from the surface of the positioning mounting plate 7 downwards, and the side part of the positioning mounting plate 7 is provided with a locking hole 73 which vertically penetrates through the cylindrical positioning hole 72; a locking column 74 is movably inserted into the locking hole 73, a chuck 75 is arranged at the outer end of the locking column 74, a pressure spring 76 is arranged between the chuck 75 and the side wall of the positioning mounting plate 7, a spherical surface 77 is arranged at the inner end of the locking column 74, and the inner end of the locking column 74 extends into the cylindrical positioning hole 72. The tapered guide hole 71 has a diameter larger than that of the cylindrical positioning hole 72 for guiding the insertion of the positioning post 60, so that the positioning post 60 can be smoothly inserted even if the positioning post 60 is slightly deviated from the cylindrical positioning hole 72.

The bottom of the positioning block 6 is fixedly connected with a positioning column 60 inserted into the positioning hole 70, a non-penetrating insertion hole 61 is formed in the positioning column 60 along a direction perpendicular to the axial direction of the positioning column, and an inclined guide surface 62 is arranged on the side surface of the bottom of the positioning column 60 facing the inner end of the locking column 74.

After the positioning post 60 is inserted into the positioning hole 70, the inner end of the locking post 74 is inserted into the insertion hole 61 of the positioning post 60 in a matching manner to lock the positioning post 60.

The inclined guide surface 62 is used for matching with a spherical surface 77 at the inner end of the locking column 74, when the positioning column 60 is inserted into the cylindrical positioning hole 72, the inclined guide surface 62 presses the spherical surface 77 to move the locking column 74 to the right, so that the positioning column 60 is inserted smoothly; when the positioning post 60 is inserted to the bottom, the locking post 74 moves to the left by the elastic force of the compression spring 76, and is inserted into the insertion hole 61 of the positioning post 60 to lock the positioning post 60.

As shown in fig. 12, in the initial state, the compression spring 76 is at the original length and is in a natural state, and the locking post 74 is inserted into the cylindrical positioning hole 72 for a distance to the left, but the spherical surface 77 at the left end of the locking post 74 is also positioned at the right side of the inclined guide surface 62 as viewed in the vertical direction, so that the positioning post 60 can be smoothly inserted into the cylindrical positioning hole 72, and the spherical surface 77 can be pressed against the right side of the inclined guide surface 62, so that the locking post 74 can be pressed to the right. In the preferred embodiment, the uppermost end of the inclined guide surface 62 is connected to the receptacle 61 by an arcuate surface to facilitate sliding of the locking post 74 from the uppermost end of the inclined guide surface 62 into the receptacle 61. When the sealed cabin 50 is installed on the adjusting platform 44 in the imaging camera bellows module 4, referring to fig. 13, the positioning column 60 on the positioning block 6 at the bottom of the sealed cabin 50 is aligned with the positioning hole 70 on the positioning installation plate 7 on the adjusting platform 44, and then inserted into the positioning hole 71 for guiding, the inclined guide surface 62 at the bottom of the positioning column 60 contacts and presses the spherical surface 77, so that the locking column 74 moves rightwards, the positioning column 60 is smoothly inserted to the bottom, at this time, under the elastic force of the pressure spring 76, the locking column 74 moves leftwards and smoothly slides into the insertion hole 61 of the positioning column 60 to lock the positioning column 60, and the sealed cabin 50 is kept stable; thereby realizing the quick positioning and installation of the sealing bin body 50 and keeping the locking. The initial position can be conveniently determined during the fluorescence imaging, and the position of the sealing bin body 50 can be kept stable during the subsequent fluorescence imaging. In the transportation process, in order to guarantee further firm, accessible screw is fixed locating piece 6 and location mounting panel 7. When the sealed cabin 50 needs to be taken out of the imaging camera module 4, the chuck 75 is pulled to draw the locking column 74 out of the insertion hole 61 of the positioning column 60, and then the sealed cabin 50 is lifted upwards.

When the device of the utility model works, the living body animal storage module with the living body animal is placed on the adjusting platform 44 of the imaging camera bellows module 4, and the position of the living body animal storage module is adjusted and fixed; connecting the anesthesia and life support system with the living animal storage module; the fluorescence imaging module 2 and the master control computer 3 are connected, the excitation light source module 1 and the fluorescence imaging module 2 are started to work, the differential adjusting head 26 and the motorized zoom lens 20 are adjusted to enable imaging to be clear, fluorescence imaging is carried out, and the master control computer 3 can display images collected by the camera 22 in real time.

While the embodiments of the invention have been disclosed above, it is not limited to the applications listed in the description and the embodiments, which are fully applicable in all kinds of fields where the invention is suitable, and further modifications may readily be made by those skilled in the art, and the invention is therefore not limited to the specific details without departing from the general concept defined by the claims and the scope of equivalents.

Claims (10)

1. A multi-spectral fluorescence imaging apparatus for living animals, comprising: the system comprises an excitation light source module, a fluorescence imaging module, a master control computer, an imaging camera bellows module, a living animal storage module and an anesthesia and life support module;

the living animal storage module is arranged inside the imaging camera bellows module and is used for storing living animals; the excitation light source module is connected with the imaging dark box module and is used for generating excitation light for irradiating living animals; the fluorescence imaging module is arranged on the imaging camera bellows module and is used for carrying out fluorescence imaging on the living animal; the anesthesia and life support module is used for providing anesthesia gas and air for maintaining living of the living animal.

2. The multi-spectral fluorescence imaging apparatus according to claim 1, wherein the excitation light source module comprises a light source, a coupling lens, a first filter and an optical fiber;

the first optical filter is arranged on a first optical filter wheel, the first optical filter wheel is arranged on a rotating shaft, and the excitation light source module further comprises a first motor for driving the rotating shaft to rotate; the optical fiber is arranged on the optical fiber sleeve, the tail end of the optical fiber is connected with a beam expander and extends into the imaging camera bellows module to irradiate the living animal.

3. The multi-spectral fluorescence imaging apparatus for living animals according to claim 2, wherein the fluorescence imaging module comprises a motorized zoom lens, a second optical filter and a camera, the motorized zoom lens extends into the imaging camera bellows module, and the camera is connected to the main control computer;

exciting light emitted by the light source sequentially passes through the coupling lens and the first optical filter and then enters the optical fiber, and then passes through the beam expanding lens at the tail end of the optical fiber and then irradiates on the living animal, and fluorescence generated after fluorescent substances in the living animal body are excited sequentially passes through the motorized zoom lens and the second optical filter and then enters the camera.

4. The multi-spectral fluorescence imaging apparatus for living animals according to claim 3, wherein the fluorescence imaging module further comprises a mounting housing disposed on the imaging camera bellows module, a second filter wheel disposed within the mounting housing, and a second motor disposed on the mounting housing for driving the second filter wheel to rotate, the second filter being mounted on the second filter wheel; the camera is connected with a focus adjusting seat, and a differential adjusting head is arranged on the focus adjusting seat.

5. The multi-spectral fluorescence imaging apparatus for living animals according to claim 4, wherein the imaging camera bellows module comprises a housing, a sealing door disposed on the housing, a support frame disposed inside the housing, a lens support plate disposed inside the housing, and an adjustment platform disposed at a bottom inside the housing.

6. The multi-spectral fluorescence imaging apparatus for living animals according to claim 5, wherein the adjustment stage is movable in a horizontal plane on a bottom plate of the housing, and the motorized zoom lens is disposed on the lens support plate;

the sealing door is connected with the box body through a hinge, and a locking mechanism is arranged on the sealing door.

7. The multi-spectral fluorescence imaging device for living animals according to claim 6, wherein the living animal storage module is disposed on the adjustment platform and comprises a sealed cabin for storing living animals, a negative pressure maintaining module for maintaining a negative pressure environment in the sealed cabin, and an air filtering module for filtering air entering and exiting the sealed cabin.

8. The multi-spectral fluorescence imaging device for living animals according to claim 7, wherein a window sheet is disposed on the top of the sealed cabin, and both the excitation light generated by the excitation light source module and the fluorescence generated by the fluorescent substance in the living animal after being excited can penetrate through the window sheet.

9. The multi-spectral fluorescence imaging device according to claim 7, wherein a plurality of positioning blocks are disposed on the bottom of the sealed capsule, and a plurality of positioning mounting plates corresponding to the positioning blocks are disposed on the adjusting platform.

10. The multispectral fluorescence imaging device for living animals according to claim 9, wherein the positioning mounting plate is provided with positioning holes, the positioning holes comprise a conical guide hole and a cylindrical positioning hole which are sequentially formed from the surface of the positioning mounting plate to the bottom, and the side part of the positioning mounting plate is provided with a locking hole which vertically penetrates through the cylindrical positioning hole; a locking column is movably inserted into the locking hole, a chuck is arranged at the outer end of the locking column, a pressure spring is arranged between the chuck and the side wall of the positioning mounting plate, a spherical surface is arranged at the inner end of the locking column, and the inner end of the locking column extends into the cylindrical positioning hole;

the bottom of the positioning block is fixedly connected with a positioning column which is inserted into the positioning hole, an insertion hole is formed in the positioning column in the direction vertical to the axial direction of the positioning column, and an inclined guide surface is arranged on the side surface of the bottom of the positioning column, facing the inner end of the locking column;

after the positioning column is inserted into the positioning hole, the inner end of the locking column is inserted into the insertion hole of the positioning column in a matched mode so as to lock the positioning column.

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