CN212015565U - Multi-mode small animal living body imaging device - Google Patents
Multi-mode small animal living body imaging device Download PDFInfo
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- CN212015565U CN212015565U CN201921988479.9U CN201921988479U CN212015565U CN 212015565 U CN212015565 U CN 212015565U CN 201921988479 U CN201921988479 U CN 201921988479U CN 212015565 U CN212015565 U CN 212015565U
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Abstract
The invention relates to the technical field of in-vivo imaging structures, in particular to a multi-mode small animal in-vivo imaging device; the imaging device comprises a box body, wherein the box body is provided with an inner cavity, a fluorescence excitation module, an X-ray source module and an up-conversion fluorescence module are arranged in the inner cavity, the X-ray source module is arranged at the bottom of the inner cavity, and the up-conversion fluorescence module is arranged at the side part of the inner cavity; an imaging module is arranged at the upper end of the box body, an object stage is arranged in the inner cavity of the box body, and a built-in anesthesia system module is arranged below the object stage; the living body specimen is arranged on the object stage, and the X-ray source module is aligned to the living body specimen on the object stage to carry out X-ray imaging; and the up-conversion fluorescence module converts the X-ray of the living body specimen into visible light and images. The imaging device can realize multiple modes of imaging such as bioluminescence imaging, fluorescence imaging, X-ray imaging, up-fluorescence conversion imaging and the like, and has the advantages of novel structural design, convenience in operation and easiness in imaging.
Description
Technical Field
The utility model relates to a live body imaging structure technical field especially relates to a multi-mode toy live body imaging device.
Background
The animal model is an important experimental method and research means in modern biomedical research, is helpful for understanding and knowing the occurrence and development rules of human diseases more conveniently and more effectively, and develops effective prevention and treatment measures according to the rules. Since about 99% of similar genes are shared by humans and mice, there are increasing studies on mice such as rats, guinea pigs, and mice in various fields such as life sciences, medical research, and drug development. In recent years, various imaging technologies play more and more important roles in animal research, various professional imaging equipment for small animals is developed, and a powerful tool is provided for scientific research.
The small animal living body imaging development cost is high, and certain technical difficulty exists. At present, the products in the domestic market are imported products, and no domestic products exist, so the improvement is urgently needed.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a novel, the convenient operation of structural design, the easy multi-mode toy live body image device that forms images.
In order to solve the technical problem, the utility model discloses the technical scheme who takes is: a multi-mode small animal living body imaging device comprises an imaging device body, wherein the imaging device body comprises a box body, an inner cavity is formed in the box body, a fluorescence excitation module, an X light source module and an up-conversion fluorescence module are arranged in the inner cavity, the X light source module is arranged at the bottom of the inner cavity, and the up-conversion fluorescence module is arranged on the side portion of the inner cavity;
an imaging module is arranged at the upper end of the box body, an object stage is arranged in the inner cavity of the box body, and a built-in anesthesia system module is arranged below the object stage;
the living body specimen is arranged on the object stage, and the X-ray source module is aligned to the living body specimen on the object stage to carry out X-ray imaging;
and the up-conversion fluorescence module converts the X-ray of the living body specimen into visible light and images.
Further, the objective table is a constant temperature objective table.
Further, the fluorescence excitation module comprises a halogen lamp device, a light source intensity detection and compensation module, an excitation light filter and a transmission device thereof, a shielding cover, an optical fiber replacement seat, and a double-gooseneck optical fiber for realizing a top annular light fiber and a local excitation light source of the planar excitation light source.
Furthermore, the X-ray source module comprises an X-ray device, an optical filter, a conversion screen and a driving and controlling device; wherein X-ray device contains X-ray lamp and radiation shield assembly, and the X-ray lamp is installed inside shield assembly, and shield assembly installs in instrument box bottom, and shield assembly opening is up, aims at objective table and top camera module.
Furthermore, the up-conversion fluorescence module consists of a laser module, a light path and a control part, wherein the laser module comprises a laser light source and a driving module and is arranged on the back outside the instrument; the light path is the optic fibre design, and laser module is connected to optic fibre one end, and the other end gets into the box through the box back, is fixed in objective table one side.
Furthermore, the anesthesia system comprises an anesthesia machine main machine, an air passage adapter, an air passage, a multi-channel anesthesia port and a mask. The anesthesia system anesthesia machine host is externally arranged and is used for loading an anesthesia reagent and generating a device capable of adjusting the concentration of the anesthesia gas; the adapter is total two sets ofly, and a set of installation is in instrument box bottom behind the back, and a set of installation is on the inside objective table of instrument box.
Furthermore, the constant-temperature electric objective table module consists of a sample table heating and temperature detecting device, a lifting and driving device and a sample table. The sample stage is heated by an electric heating film.
Further, the imaging module comprises a camera, a lens module, an emission light filter wheel module, a macro imaging module, a light avoiding cover and a fixing plate.
The imaging device is internally provided with system partial modules such as an X-ray source, an upper fluorescence excitation light path, an anesthesia system and the like, can realize multiple modes of imaging such as bioluminescence imaging, fluorescence imaging, X-ray imaging, up-conversion fluorescence imaging and the like, and has the advantages of novel structural design, convenience in operation and easiness in imaging.
Drawings
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
FIG. 1 is a schematic structural diagram of a multi-mode small-animal in-vivo imaging device of the present invention;
FIG. 2 is a schematic view of another perspective configuration of a multi-mode small-animal in-vivo imaging device of the present invention;
fig. 3 is a schematic diagram of the overall appearance structure of a multimode small-animal living body imaging device of the invention.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be implemented in other ways different from the specific details set forth herein, and one skilled in the art may similarly generalize the present invention without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
Fig. 1 is a schematic structural diagram of a multimode small-animal in-vivo imaging device according to the present invention; fig. 2 is a schematic view of another perspective structure of the multimode small-animal in-vivo imaging device according to the invention; fig. 3 is a schematic diagram showing the overall appearance of a multimode small-animal living body imaging device according to the present invention. The imaging device comprises a box body 7, wherein the box body 7 is provided with an inner cavity, a fluorescence excitation module 3, an X-ray source module 4 and an up-conversion fluorescence block 8 are arranged in the inner cavity, the X-ray source module 4 is arranged at the bottom of the inner cavity, and the up-conversion fluorescence block 8 is arranged at the side part of the inner cavity; the upper end of the box body 7 is provided with an imaging module 1, an object stage 6 is arranged in the inner cavity 1 of the box body, and a built-in anesthesia system module 5 is arranged below the object stage 6; illustratively, the stage 6 is a constant temperature stage.
The living body specimen is arranged on the object stage 6, and the X-ray source module 4 is aligned with the living body specimen on the object stage to carry out X-ray imaging; the up-conversion fluorescence module 8 converts the X-ray of the living body specimen into visible light and images.
In the invention, the fluorescence excitation module 3 comprises a halogen lamp device, a light source intensity detection and compensation module, an excitation light filter and a transmission device thereof, a shielding cover, an optical fiber replacement seat, and a double-gooseneck optical fiber for realizing a top annular light fiber and a local excitation light source of a planar excitation light source.
The halogen lamp device comprises a halogen lamp bulb, a reflective light-gathering cover and a control circuit; each light source has an attenuation process, a light source intensity detection device is arranged between the optical filter and the excitation light incidence end of the optical fiber, and the optical fiber fixing seat is used for detecting the intensity of excitation light and compensating light attenuation through control software so as to ensure the consistency of the intensity of the excitation light in different time periods; the optical filter is arranged between the halogen lamp and the optical fiber fixing seat, 4 exciting light optical filters are arranged on the instrument, and the optical filter with a certain exciting wavelength can be moved between the halogen lamp and the optical fiber fixing seat through the transmission device to generate exciting light with a corresponding wavelength; the shielding cover is used for shielding external interference light and preventing the external interference light from entering the box body through a light path to influence the imaging effect; the light outlet end of the annular lamp optical fiber is fixed at the top of the instrument box body, the light inlet end of the annular lamp optical fiber is inserted into the optical fiber fixing seat and is fixed by a hand-screwed type compression screw to be used as a global excitation light source. The double-gooseneck optical fiber is a replaceable accessory, when local light excitation is needed or negative contrast is needed, the light entering end of the annular lamp optical fiber can be taken down from the optical fiber fixing seat, the light entering end of the double-gooseneck optical fiber is inserted into the optical fiber fixing seat and is fixed by screwing the compression screw, then the shape of the gooseneck is manually adjusted, the irradiation position and the irradiation angle of the local excitation light are adjusted, and local fluorescence excitation imaging or negative contrast imaging can be achieved.
In the present invention, the X-ray source module includes an X-ray device, a filter, a conversion screen, and a driving and controlling device; wherein X-ray device contains X-ray lamp and radiation shield assembly, and the X-ray lamp is installed inside shield assembly, and shield assembly installs in instrument box bottom, and shield assembly opening is up, aims at objective table and top camera module.
The X-ray source module comprises an X-ray device, an optical filter, a conversion screen and a driving and controlling device, wherein the X-ray device comprises an X-ray lamp and a radiation shielding device, the X-ray lamp is arranged in the shielding device, the shielding device is arranged at the bottom of the instrument box body, the opening of the shielding device faces upwards and is aligned with the objective table and the top camera module, X-ray imaging is facilitated, and radiation injury to instrument users is avoided; the optical filter is arranged on the optical filter rotating disc of the imaging module, and when the X-ray is imaged, the optical filter rotating disc rotates the X-ray optical filter to the lower part of the lens for filtering the X-ray, so that the damage of the X-ray to the CCD camera is avoided, and the CCD camera is protected; a conversion screen placing position is arranged on the objective table and is between the imaging module and the experimental sample, when X-ray imaging is used, the sample is placed on the objective table firstly, and then the conversion screen is installed, so that X-rays penetrating through the sample can be converted into visible light to further image; the driving device is arranged on the back of the outside of the box body and is connected with the X-ray through a wire, the control device is a handheld self-resetting switch, when the X-ray is imaged, the switch is pressed down firstly, the control device turns on the X-ray source, then the X-ray source is shot through the computer control instrument, after the shooting is completed, the switch is released by hands, and the control device automatically turns off the X-ray source.
The fluorescence conversion module consists of a laser module, a light path and a control part, wherein the laser module comprises a laser light source and a driving module and is arranged on the back outside the instrument; the light path is the optic fibre design, and laser module is connected to optic fibre one end, and the other end gets into the box through the box back, is fixed in objective table one side.
In the fluorescence imaging, a halogen lamp and a narrow-band filter are matched to serve as an excitation light source, the excitation light with high energy is converted into emission light with low energy through a fluorescence marker, and imaging is carried out.
The X-ray imaging is based on the difference of tissue density and thickness of a living body sample, and the X-ray passing through the living body sample is converted into visible light through a conversion screen and imaged.
The upconversion fluorescence is to use laser as an excitation light source, convert excitation light with low energy into emission light with high energy through a fluorescent marker, and image.
The fluorescence conversion module consists of a laser module, a light path and a control part, wherein the laser module comprises a laser light source and a driving module and is arranged on the back outside the instrument; the light path is designed by optical fibers, one end of each optical fiber is connected with the laser module, and the other end of each optical fiber enters the box body through the back of the box body and is fixed on one side of the objective table; the control part is controlled by a main control board arranged on the back outside the box body.
The anesthesia system comprises an anesthesia machine main machine, a gas path adapter, a gas path, a multi-channel anesthesia port and a mask. The anesthesia system anesthesia machine host is externally arranged and is used for loading an anesthesia reagent and generating a device capable of adjusting the concentration of the anesthesia gas; the adapter is total two sets ofly, and a set of installation is in instrument box bottom behind the back, and a set of installation is on the inside objective table of instrument box.
The gas circuit is connected with the adapter through a hose and is realized, including two gas circuits, is the admission passage all the way, and anesthesia machine gas outlet is connected to one end, and one end is connected to the inlet end of the inside multichannel anesthesia port of instrument box. One path is an air outlet passage, one end of the air outlet passage is connected to an air outlet end of a multi-channel anesthesia port in the instrument box body, and the other end of the air outlet passage is connected to an activated carbon box outside the instrument; the multichannel anesthesia port is provided with an air inlet, an air outlet and a plurality of anesthesia ports, and one anesthesia port is connected with one face mask.
During the use, first add the anesthesia reagent in the anesthesia machine, and adjust the anesthetic gas dose, with anesthesia machine gas outlet intercommunication induction box, make anesthetic gas add in induction box, then place the mouse and place anesthesia in induction box, then move the mouse after the anesthesia to the inside multichannel anesthesia port of instrument box, go deep into within the face guard with the mouse mouth, communicate anesthesia machine gas outlet to the box gas circuit again, anesthetic gas gets into the box through the inlet channel, and send anesthetic gas to the mouse nasal part through multichannel anesthesia port and face guard, along with the mouse breathes, anesthetic gas lasts and gets into in the mouse, and then realize the long-time continuous anesthesia to the mouse, unnecessary anesthetic gas is discharged outside the instrument box through the outlet channel, be absorbed by the active carbon box.
The constant-temperature electric objective table module consists of a sample table heating and temperature detecting device, a lifting and driving device and a sample table. The sample stage is heated by an electric heating film.
The constant-temperature electric objective table module consists of a sample table heating and temperature detecting device, a lifting and driving device and a sample table.
The sample stage is heated by an electric heating film, such as a polyimide heating film and the like, and is fixed on two sides of the back of the sample stage, the temperature detection module is also fixed on the back of the sample stage, and the temperature detection module and the sample stage are both connected with a main control board at the back of the box body through a lead, so that the timely detection and control of the temperature can be realized; the lifting and driving device comprises a screw rod device and a driving motor, the screw rod device is fixed in the box body, the driving motor is fixed at the top of the screw rod device and is arranged at the top outside the box body, and the driving motor is controlled to rotate through the main control board so as to control the height of the sample table; the sample stage comprises an objective table, a positioning film and a glass plate, wherein the objective table is a metal plate, rapid heat conduction and bearing are facilitated, the middle of the objective table is hollow, the center of the hollow part is right opposite to the central axis of the camera and the central axis of the lens, and the hollow part is filled with the glass plate and is convenient for X-ray transmission. The positioning film is provided with a positioning mark, and the center of the positioning mark is superposed with the central axis of the camera and the lens on the fixed objective table and the glass plate and is used for positioning the position of a shooting sample in a visual field. Wherein the objective table is a metal plate with fast heat conduction, such as an aluminum plate or a copper plate, the glass plate material is transparent organic glass, inorganic glass or a transparent plastic product, and the positioning film is a plastic product or an organic glass product, such as PS, PC and the like.
The imaging module comprises a camera, a lens module, an emission light filter wheel module, a micro-distance imaging module, a light avoiding cover and a fixing plate.
The imaging module comprises a camera, a lens module, a transmission light filter wheel module, a micro-distance imaging module, a light avoiding cover and a fixing plate. The camera is a depth refrigeration CCD camera and is fixed on the fixing plate through a screw; the lens is positioned below the fixed plate, is arranged on an interface at the front end of the CCD camera and is fixed below the fixed plate through a fixed frame, and the driving and positioning modules such as lens focusing and aperture and the like are fixed on the lens or the fixed plate and are used for adjusting different vision fields and different exposure intensities; the emission light filter wheel is a multi-hole position light filter wheel and is fixed below the fixing plate through a support, one emission light filter can be placed on each hole position, the emission light filter wheel is driven to rotate through a motor, any emission light filter on the hole is positioned through a positioning sensor, so that each hole position can rotate to be right below the lens, and imaging of different emission light filters is facilitated. The fixed plate is fixed on the top of the box body, the camera part above the fixed plate is arranged outside the box body, and the modules such as the lens on the lower part of the fixed plate are all arranged on the top in the box body.
The box is closed box, and box open-top for camera module's installation, the preceding box door that sets up of box is used for the sample operation, is fixed with rubber sealing device on the box door, and the box is closed after, with the box totally enclosed.
The product of the invention is also provided with a software system, the control system 2 controls the working state of the whole imaging device, and the upper computer control and analysis program is used for controlling the instrument parameters, shooting and imaging and analyzing the images.
The imaging device is internally provided with system partial modules such as an X-ray source, an upper fluorescence excitation light path, an anesthesia system and the like, can realize multiple modes of imaging such as bioluminescence imaging, fluorescence imaging, X-ray imaging, up-conversion fluorescence imaging and the like, and has the advantages of novel structural design, convenience in operation and easiness in imaging.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.
Claims (8)
1. The multi-mode small animal living body imaging device is characterized by comprising a box body, wherein the box body is provided with an inner cavity, a fluorescence excitation module, an X-ray source module and an up-conversion fluorescence module are arranged in the inner cavity, the X-ray source module is arranged at the bottom of the inner cavity, and the up-conversion fluorescence module is arranged at the side part of the inner cavity;
an imaging module is arranged at the upper end of the box body, an object stage is arranged in the inner cavity of the box body, and a built-in anesthesia system module is arranged below the object stage;
the living body specimen is arranged on the object stage, and the X-ray source module is aligned to the living body specimen on the object stage to carry out X-ray imaging;
and the up-conversion fluorescence module converts the X-ray of the living body specimen into visible light and images.
2. The imaging apparatus of claim 1, wherein the stage is a constant temperature stage.
3. The imaging device according to claim 2, wherein the fluorescence excitation module comprises a halogen lamp device, a light source intensity detection and compensation module, an excitation light filter and its transmission device, a shielding cover, a fiber replacement seat, a top ring-shaped light fiber for realizing a planar excitation light source, and a double-gooseneck fiber for realizing a local excitation light source.
4. The imaging device of claim 3, wherein the X-ray source module comprises an X-ray device, a filter, a conversion screen, and a driving and controlling device; wherein X-ray device contains X-ray lamp and radiation shield assembly, and the X-ray lamp is installed inside shield assembly, and shield assembly installs in the instrument camera bellows bottom, and shield assembly opening is up, aims at objective table and top camera module.
5. The imaging device of claim 4, wherein the upconversion fluorescence module comprises a laser module, an optical path and a control part, wherein the laser module comprises a laser light source and a driving module and is arranged on the back outside the instrument; the light path is designed by optical fiber, one end of the optical fiber is connected with the laser module, and the other end of the optical fiber enters the camera bellows through the back of the camera bellows and is fixed on one side of the objective table.
6. The imaging device of claim 5, wherein the anesthesia system comprises an anesthesia machine host, a gas path adapter, a gas path, a multi-channel anesthesia port and a mask; the anesthesia system anesthesia machine host is externally arranged and is used for loading an anesthesia reagent and generating a device capable of adjusting the concentration of the anesthesia gas; the adapter is total two sets ofly, and a set of installation is in instrument camera bellows back bottom, and a set of installation is on the inside objective table of instrument camera bellows.
7. The imaging device of claim 6, wherein the constant temperature motorized stage module comprises a stage heating and temperature detecting device, a lifting and driving device, and a stage; the sample stage is heated by an electric heating film.
8. The imaging apparatus of claim 7, wherein the imaging module comprises a camera, a lens module, an emission light filter wheel module, a macro imaging module, a light avoiding mask, and a fixing plate.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921988479.9U CN212015565U (en) | 2019-11-18 | 2019-11-18 | Multi-mode small animal living body imaging device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921988479.9U CN212015565U (en) | 2019-11-18 | 2019-11-18 | Multi-mode small animal living body imaging device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112998660A (en) * | 2021-03-01 | 2021-06-22 | 无锡博医光电技术有限公司 | Biological safety bin for small animal living body fluorescence imaging instrument and fluorescence imaging instrument |
| CN114224557A (en) * | 2021-12-17 | 2022-03-25 | 广州博鹭腾生物科技有限公司 | Gas anesthesia structure and gas circulation system for imaging instrument |
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2019
- 2019-11-18 CN CN201921988479.9U patent/CN212015565U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112998660A (en) * | 2021-03-01 | 2021-06-22 | 无锡博医光电技术有限公司 | Biological safety bin for small animal living body fluorescence imaging instrument and fluorescence imaging instrument |
| CN114224557A (en) * | 2021-12-17 | 2022-03-25 | 广州博鹭腾生物科技有限公司 | Gas anesthesia structure and gas circulation system for imaging instrument |
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