CN211477653U

CN211477653U - Pressure-maintaining, heat-preserving, sampling and transferring system for benthos

Info

Publication number: CN211477653U
Application number: CN202020118099.6U
Authority: CN
Inventors: 金永平; 刘广平; 彭佑多; 万步炎; 刘平; 谢焜
Original assignee: Hunan University of Science and Technology
Current assignee: Hunan University of Science and Technology
Priority date: 2020-01-19
Filing date: 2020-01-19
Publication date: 2020-09-11
Anticipated expiration: 2030-01-19

Abstract

The utility model discloses a pressure-maintaining, heat-preserving, sampling and transferring system for benthos, which comprises a pressure-maintaining assembly, a sampling assembly and a circuit barrel; the sampling assembly is inserted into the pressure maintaining assembly, the sampling assembly is connected with the culture kettle, an inlet sealing assembly is arranged at an inlet in the middle of the pressure maintaining assembly, a heat preservation assembly is bonded on the outer wall in the middle of the pressure maintaining assembly, a pressure compensation device and a bait cylinder are arranged on the outer wall at the bottom of the pressure maintaining assembly, the pressure compensation device is communicated with the pressure maintaining assembly, and the bait cylinder is communicated with the pressure maintaining assembly through a switch valve; a temperature sensor and a pressure sensor are arranged on the inner wall of the pressure maintaining assembly; the circuit barrel is installed on the outer wall of the pressure maintaining assembly, a power supply and a controller are arranged in the circuit barrel, the power supply supplies power to the heat preservation assembly, the controller, the temperature sensor and the pressure sensor, and the controller is connected with the temperature sensor, the pressure sensor and the heat preservation assembly respectively. The utility model discloses can realize traping the benthos and cultivate with pressurize heat preservation, can effectively guarantee the vital sign of benthos normal position.

Description

Pressure-maintaining, heat-preserving, sampling and transferring system for benthos

Technical Field

The utility model relates to a deep sea biology sampling device, in particular to a pressure-maintaining, heat-preserving, sampling and transferring system for benthos.

Background

The total area of the oceans all over the world is about 3.6 billion square kilometers, which accounts for 71 percent of the surface area of the earth, and the seabed not only contains abundant mineral resources, but also breeds a variety of unknown biological resources. The exploration of submarine biological resources is an important link for revealing human life secret and gene origin, and can greatly promote the development of life science. However, the research on the benthos sampling tool is mostly concentrated on trawling, grab buckets, trapping type large-scale organism samplers and the like at present, and the collected benthos is dead in the process of returning to a mother ship on the water surface from the seabed because the original living environment of the benthos cannot be maintained in the process from sampling to experiment, which is very unfavorable for researching the species characteristics of the benthos. Therefore, a pressure-maintaining, heat-preserving, sampling and transferring system for the benthos is developed, the in-situ characteristics of the benthos are ensured as much as possible, the system has important significance for maintaining the original structure and properties of the benthos, and is vital to the truth and credibility of the benthos detection data.

Disclosure of Invention

In order to solve the technical problem, the utility model provides a simple structure, convenient operation, the reliable operation benthos pressurize heat preservation sampling transfer system.

The utility model provides a technical scheme of above-mentioned problem is: a pressure-maintaining, heat-preserving, sampling and transferring system for benthos comprises a pressure-maintaining assembly for maintaining pressure, a sampling assembly for sampling benthos, a circuit cylinder for controlling, a temperature sensor for acquiring the temperature in the pressure-maintaining assembly, a pressure sensor for acquiring the pressure in the pressure-maintaining assembly and a culture kettle for transferring the benthos; the sampling assembly is inserted into the pressure maintaining assembly, the sampling assembly is connected with the culture kettle, an inlet sealing assembly is arranged at an inlet in the middle of the pressure maintaining assembly, a heat preservation assembly for heat preservation is bonded on the outer wall in the middle of the pressure maintaining assembly, a pressure compensation device for keeping the pressure balance in the pressure maintaining assembly and a bait tube for releasing bait are arranged on the outer wall at the bottom of the pressure maintaining assembly, the pressure compensation device is communicated with the pressure maintaining assembly, and the bait tube is communicated with the pressure maintaining assembly through a switch valve; a temperature sensor and a pressure sensor are arranged on the inner wall of the pressure maintaining assembly; the circuit barrel is installed on the outer wall of the pressure maintaining assembly, a power supply and a controller are arranged in the circuit barrel, the power supply supplies power to the heat insulating assembly, the controller, the temperature sensor and the pressure sensor, and the controller is connected with the temperature sensor, the pressure sensor and the heat insulating assembly respectively.

According to the pressure-maintaining, heat-preserving, sampling and transferring system for the benthos, the pressure-maintaining assembly comprises a pressure-maintaining cylinder, a square stop bolt and a high-pressure valve I, a plurality of square stop bolt holes are radially formed in the side wall of the top of the pressure-maintaining cylinder, and a square stop bolt is arranged in each square stop bolt hole; keep off and tie the box through I fixed mounting of bolt corresponding to square fender bolt department on the pressurize section of thick bamboo outer wall, square fender bolt with keep off and tie between the relative terminal surface of box and be connected through compression spring I, square fender bolt can be in square fender bolt downthehole removal under compression spring I's effect, keep off to be equipped with on the bolt box and be used for carrying out spacing spacer pin to square fender bolt, keep off a section of thick bamboo inner chamber bottom and be connected with high-pressure valve I through high-pressure pipe I.

The pressure-maintaining, heat-preserving, sampling and transferring system for the benthos comprises a sampling cylinder, a sampling rod, an upper end cover and a lower end cover, wherein the upper end of the sampling cylinder is fixed on the lower end surface of the upper end cover, the lower end of the sampling cylinder is fixed on the upper end surface of the lower end cover, the sampling rod is fixed on the upper end surface of the upper end cover through a bolt II, and an annular groove is formed in the upper end part of the sampling rod; a sealing ring I is arranged on the side wall of the upper end cover, a through hole I is formed in the center of the lower end cover, and a sealing ring II is arranged on the side wall of the lower end cover; the upper part of the side wall of the sampling cylinder is provided with a circular hole I, the lower part of the side wall of the sampling cylinder is provided with a circular hole II, and the upper end part of the sampling cylinder is provided with a circular through hole; the diameter of the outlet of the pressure maintaining cylinder is the same as the diameter of the circular hole I, the circular hole II and the circular through hole.

In the pressure-maintaining, heat-preserving, sampling and transferring system for the benthos, the inlet sealing assembly comprises a sealing cylinder, a flap valve seat, a flap valve cover, a flap shaft, a sealing ring III, a torsion spring and a sleeve; the flap valve seat is connected with the sealing cylinder inlet in a sealing way through a bolt III, a hinge lug is arranged on the flap valve seat, a flap shaft is fixed on the hinge lug, a flap valve cover is hinged with the flap valve seat through the flap shaft and the hinge lug to open and close the sealing cylinder inlet, the flap valve cover and the flap valve seat are sealed through a sealing ring III, and a torsion spring is also arranged on the flap shaft; the sleeve is arranged on the flap valve cover, and the sealing cylinder is provided with a trigger mechanism for limiting the sleeve and the flap valve cover; the trigger mechanism comprises a trigger rod I, one end of the trigger rod I is provided with a trigger rope mounting hole I and is connected with the trigger rope I through the trigger rope mounting hole I, the other end of the trigger rod I penetrates through the trigger rod mounting hole I on the side wall of the sealing cylinder and then is inserted into the sleeve, the trigger rod I and the trigger rod mounting hole I are sealed through a sealing ring IV, and the side wall of the sealing cylinder is provided with a limiting mechanism I for limiting the trigger rod I; the outlet of the sealing cylinder is communicated with the inlet in the middle of the pressure maintaining assembly 1; the sleeve on the flap valve cover and the central line of the trigger rod I on the trigger mechanism are on the same straight line.

In the pressure-maintaining, heat-preserving, sampling and transferring system for the benthos, the pressure compensation device comprises a pressure-resistant cylinder, a piston, a compensator end cover and an inflation valve; the compensator end cover is hermetically arranged at an opening at the top of the pressure-resistant cylinder; the piston is arranged in the pressure-resistant cylinder; the compensator end cover is provided with a high-pressure pipe connecting hole and is communicated with the pressure maintaining cylinder through a high-pressure pipe II; the bottom of the pressure-resistant cylinder is provided with a through hole II which is connected with the inflation valve.

In the pressure-maintaining, heat-preserving, sampling and transferring system for the benthos, the bait cylinder comprises a pressure-resistant cylinder body, a bait cylinder bottom cover and a filter plate; the bait cylinder bottom cover is arranged at the bottom of the pressure-resistant cylinder body through a bolt IV; bait is arranged in the pressure-resistant cylinder body; the top of the pressure-resistant cylinder body is provided with a filter plate, the filter plate is provided with filter holes, and the bait cylinder is communicated with the inner cavity of the pressure-maintaining cylinder through a high-pressure pipe III and a switch valve; the diameter of the filter hole on the filter plate is smaller than the inner diameter of the high-pressure pipe III.

In the pressure-maintaining, heat-preserving, sampling and transferring system for the benthos, the switch valve comprises an inlet interface, an outlet interface, a sealing ring V, a valve body and a trigger rod II; the lower portion of the valve body is provided with an inlet interface, the inlet interface is communicated with a bait barrel through a high-pressure pipe III, the upper portion of the valve body is provided with an outlet interface, the outlet interface is communicated with a pressure maintaining barrel through a high-pressure pipe IV, the middle portion of the valve body is provided with a cavity used for communicating the inlet interface with the outlet interface, one end of the cavity is provided with an opening, one end of the trigger rod II is provided with a trigger rope mounting hole II, the trigger rope II is connected with the trigger rope mounting hole II through the trigger rope mounting hole II, the other end of the trigger rod II is transversely inserted into the cavity from the opening of the cavity and can slide in the cavity, the opening of the cavity is provided with a limiting mechanism II used for limiting the trigger rod II, the size of the trigger rod II is matched with the size of the inner cavity, the trigger rod II.

The pressure-maintaining, heat-preserving, sampling and transferring system for the benthos comprises two groups of heat-preserving assemblies, wherein the heat-preserving assemblies are bonded with the outer wall of the pressure-maintaining cylinder through heat-conducting silica gel; the cold end of the semiconductor refrigeration piece is connected with the outer wall of the pressure maintaining cylinder through a heat conducting plate, and the hot end of the semiconductor refrigeration piece is contacted with the seawater through a radiating fin; each stage of semiconductor refrigeration piece is connected with each electrode holder through coupling nut respectively, establishes ties through the electric wire between each electrode holder, and the electric wire is connected with the circuit section of thick bamboo through watertight cable I.

In the pressure-maintaining, heat-preserving, sampling and transferring system for the benthos, the culture kettle comprises a high-pressure cylinder, an end cover and a sealing plug; the bottom opening of the high-pressure cylinder is sealed by an end cover, a sealing plug is arranged in the middle of the end cover, and the sealing plug and the end cover are sealed by a sealing ring VI; the culture kettle is connected with the sampling cylinder through a sealing plug, and an insertion cavity for inserting the top of the sampling rod is arranged in the middle of the bottom of the sealing plug; the side wall of the bottom of the sealing plug is provided with a plurality of stop bolt holes which are arranged along the radial direction, each stop bolt hole is internally provided with a stop bolt, the front end of each stop bolt extends into the insertion cavity, and the rear side of each stop bolt is fixed with a bolt V; the stop bolt is connected with the end face, opposite to the bolt V, of the bolt V through a compression spring II, and the stop bolt can move in the stop bolt hole under the action of the compression spring II.

The beneficial effects of the utility model reside in that:

1. the utility model discloses a benthos pressurize heat preservation sampling transfer system can realize traping and pressurize heat preservation cultivation to benthos, can guarantee the vital sign of benthos normal position effectively.

2. The utility model discloses a benthos pressurize heat preservation sampling transfer system can realize and cultivates the cauldron butt joint, shifts benthos pressurize heat preservation.

3. The utility model discloses a benthos pressurize heat preservation sampling transfer system adopts two sets of independent semiconductor refrigeration subassemblies, can control its work switch according to the environment needs, and the heat preservation scope is wide.

4. The utility model discloses a benthos pressurize heat preservation sampling transfer system simple structure, compactness, easy and simple to handle, be fit for carrying on lander, manned submersible, unmanned submersible.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention.

Fig. 2 is a state diagram before sampling of the present invention.

Fig. 3 is a state diagram after sampling of the present invention.

Fig. 4 is a schematic structural view of the pressure maintaining assembly of the present invention.

Fig. 5 is a schematic structural diagram of the sampling assembly of the present invention.

Fig. 6 is a schematic structural view of the opening state of the inlet seal assembly of the present invention.

Fig. 7 is a schematic structural view of the inlet seal assembly in a closed state according to the present invention.

Fig. 8 is a schematic structural diagram of the pressure compensation device of the present invention.

Fig. 9 is a schematic view of the bait cartridge of the present invention.

Fig. 10 is a schematic structural diagram of the switching tube valve of the present invention.

Fig. 11 is a schematic structural view of the heat preservation assembly of the present invention.

FIG. 12 is a schematic structural view of the culture kettle of the present invention.

FIG. 13 is an enlarged view of the sealing plug of the culture vessel according to the present invention.

Fig. 14 is a block diagram of the circuit structure of the present invention.

Fig. 15 is a circuit diagram of the controller of the present invention.

Fig. 16 is a schematic structural view of the butt joint of the sampling assembly and the culture kettle of the present invention.

Fig. 17 is a state diagram of the transfer process of the present invention.

Detailed Description

The present invention will be further described with reference to the accompanying drawings and examples.

As shown in fig. 1-3, a pressure-maintaining, heat-preserving, sampling and transferring system for benthos is characterized in that: the device comprises a pressure maintaining assembly 1 for maintaining pressure, a sampling assembly 3 for sampling benthos, a circuit cylinder 15 for control, a temperature sensor 14 for acquiring the temperature in the pressure maintaining assembly 1, a pressure sensor 13 for acquiring the pressure in the pressure maintaining assembly 1 and a culture kettle 9 for transferring the benthos; the sampling assembly 3 is inserted into the pressure maintaining assembly 1, the sampling assembly 3 is connected with the culture kettle 9, an inlet sealing assembly 2 is arranged at an inlet in the middle of the pressure maintaining assembly 1, a heat preservation assembly 12 for heat preservation is bonded on the outer wall in the middle of the pressure maintaining assembly 1, a pressure compensation device 4 for keeping the pressure balance in the pressure maintaining assembly 1 and a bait cylinder 6 for releasing bait are arranged on the outer wall at the bottom of the pressure maintaining assembly 1, the pressure compensation device 4 is communicated with the pressure maintaining assembly 1, and the bait cylinder 6 is communicated with the pressure maintaining assembly 1 through a switch valve 7; a temperature sensor 14 and a pressure sensor 13 are arranged on the inner wall of the pressure maintaining assembly 1; the circuit barrel 15 is installed on the outer wall of the pressure maintaining assembly 1, a power supply and a controller are arranged in the circuit barrel 15, the power supply supplies power to the heat preservation assembly 12, the controller, the temperature sensor 14 and the pressure sensor 13, and the controller is connected with the temperature sensor 14, the pressure sensor 13 and the heat preservation assembly 12 respectively.

As shown in fig. 4, the pressure maintaining assembly 1 comprises a pressure maintaining cylinder 101 and a square blocking bolt 102, wherein a plurality of square blocking bolt holes 104 are radially arranged on the side wall of the top of the pressure maintaining cylinder 101, the square blocking bolt 102 is arranged in each square blocking bolt hole 104, and a sealing ring vii 108 is arranged between each square blocking bolt hole 104 and the square blocking bolt 102; keep off on the outer wall of a section of thick bamboo 101 and correspond to square fender bolt 102 department and have a fender bolt box 106 through I105 fixed mounting of bolt, square fender bolt 102 with keep off and be connected through I107 compression spring between the terminal surface that bolt box 106 is relative, square fender bolt 102 can remove in square fender bolt hole 104 under the effect of compression spring I107, keep off and be equipped with on the bolt box and be used for carrying out spacing spacer pin 105 to square fender bolt, keep off a section of thick bamboo 101 inner chamber bottom and be connected with high-pressure valve I17 through high-pressure pipe I103.

As shown in fig. 5, the sampling assembly 3 includes a sampling cylinder 301, a sampling rod 302, an upper end cap 303 and a lower end cap 304, the upper end of the sampling cylinder 301 is fixed on the lower end face of the upper end cap 303, the lower end of the sampling cylinder 301 is fixed on the upper end face of the lower end cap 304, the sampling rod 302 is fixed on the upper end face of the upper end cap 303 through a bolt ii 305, and the upper end of the sampling rod 302 is provided with an annular groove 311; a sealing ring I306 is arranged on the side wall of the upper end cover 303, a through hole I is arranged in the center of the lower end cover 304, and a sealing ring II 307 is arranged on the side wall of the lower end cover 304; a circular hole I308 is formed in the upper portion of the side wall of the sampling cylinder 3, a circular hole II309 is formed in the lower portion of the side wall of the sampling cylinder 3, and a circular through hole 310 is formed in the upper end portion of the sampling cylinder 3; the diameter of the outlet of the pressure maintaining cylinder 101 is the same as that of the circular hole I308, the circular hole II309 and the circular through hole 310, the diameter is 68mm, and the entrance of macroorganisms in the full-sea deep environment can be basically met; the pressure maintaining cylinder 101 is made of titanium alloy materials and can bear 120Mpa of ultrahigh pressure.

As shown in fig. 6 and 7, the inlet sealing assembly 2 includes a sealing cylinder 207, a flap valve seat 201, a flap valve cover 202, a flap shaft 205, a sealing ring iii 206, a torsion spring 205, and a sleeve 203; the flap valve seat 201 is connected with an inlet of the sealing cylinder 207 in a sealing manner through a bolt III 208, a hinge lug is arranged on the flap valve seat 201, a flap shaft 205 is fixed on the hinge lug, the flap valve cover 202 is hinged with the flap valve seat 201 through the flap shaft 205 and the hinge lug to open and close the inlet of the sealing cylinder 207, the flap valve cover 202 and the flap valve seat 201 are sealed through a sealing ring III 206, and a torsion spring 204 is further arranged on the flap shaft 205; the sleeve 203 is arranged on the flap valve cover 202, and the sealing cylinder 207 is provided with a trigger mechanism 5 for limiting the sleeve 203 and the flap valve cover 202; the trigger mechanism 5 comprises a trigger rod I501, one end of the trigger rod I501 is provided with a trigger rope mounting hole I502, the trigger rod I is connected with a trigger rope I8 through the trigger rope mounting hole I502, the other end of the trigger rod I501 penetrates through the trigger rod mounting hole I on the side wall of the sealing cylinder 507 and then is inserted into the sleeve 203, the trigger rod I501 and the trigger rod mounting hole I are sealed through a sealing ring IV, and the side wall of the sealing cylinder 507 is provided with a limiting mechanism I for limiting the trigger rod I501; an outlet of the sealing cylinder 207 is communicated with an inlet in the middle of the pressure maintaining assembly 1; the sleeve 203 on the flap valve cover 202 is on the same straight line with the central line of the trigger lever I501 on the trigger mechanism 5. When the flap valve cover 202 is required to be closed, the trigger rod I501 is pulled by the trigger rope I8 to move left, so that the trigger rod I501 is separated from the sleeve 203 on the flap valve cover 202, and the flap valve cover 202 is closed under the action of the torsion spring 204 and covers the inlet of the sealing cylinder 207.

As shown in fig. 8, the pressure compensation device 4 includes a pressure-resistant cylinder 403, a piston 402, a compensator end cap 404 and an inflation valve 401; the compensator end cover 404 is hermetically arranged at the opening at the top of the pressure-resistant cylinder 403; the piston 402 is placed in the pressure-resistant cylinder 403; the compensator end cover 404 is provided with a high-pressure pipe connecting hole and is communicated with the pressure maintaining cylinder 101 through a high-pressure pipe II 10; the bottom of the pressure-resistant cylinder 403 is provided with a through hole II which is connected with the inflation valve 401.

As shown in fig. 9, the bait cartridge 6 comprises a pressure-resistant cylinder 601, a bait cartridge bottom cover 604, and a filter plate 602; the bait cylinder bottom cover 604 is arranged at the bottom of the pressure-resistant cylinder body 601 through a bolt IV; bait is arranged in the pressure-resistant cylinder body 601; the top of the pressure-resistant cylinder body 601 is provided with a filter plate 602, the filter plate 602 is provided with a filter hole 603, and the bait cylinder 6 is communicated with the inner cavity of the pressure-maintaining cylinder 101 through a high-pressure pipe III and a switch valve 7; the diameter of the filter holes 603 on the filter plate 602 is smaller than the inner diameter of the high-pressure pipe III.

As shown in fig. 10, the switching valve 7 includes an inlet port 706, an outlet port 703, a sealing ring v 702, a valve body 704, and a trigger rod ii 701; an inlet interface 706 is arranged at the lower part of the valve body 704, the inlet interface 706 is communicated with the bait barrel 6 through a high-pressure pipe III, an outlet interface 703 is arranged at the upper part of the valve body 704, an outlet interface 707 is communicated with the pressure-maintaining barrel 101 through a high-pressure pipe IV 11, a cavity for communicating the inlet interface 706 with the outlet interface 703 is arranged at the middle part of the valve body 704, one end of the cavity is open, a trigger rope mounting hole II 705 is arranged at one end of the trigger rod II 701, the trigger rope II 801 is connected through a trigger rope mounting hole II 705, the other end of the trigger rod II 701 is transversely inserted into the cavity from the opening of the cavity and can slide in the cavity, a limiting mechanism II used for limiting the trigger rod II 701 is arranged at the opening of the cavity, the size of the trigger rod II 701 is matched with that of the inner cavity, the trigger rod II 701 and the valve body 704 are sealed through a sealing ring V702, an annular groove is formed in the middle of the trigger rod II 701, and the annular groove is opposite to the inlet interface 706 and the outlet interface 703. Fig. 10 is a state diagram when the switch valve 7 is opened, the inlet port 706 and the outlet port 703 are communicated through an annular groove in the middle of the trigger rod ii 701, and when the switch valve 7 needs to be closed, the trigger rod ii 701 is pulled by the trigger rope ii 801 to move left, so that the annular groove in the middle of the trigger rod ii 701 is dislocated with the inlet port 706 and the outlet port 703, and the plugging can be realized.

As shown in fig. 11, there are two groups of the thermal insulation assemblies 12, the thermal insulation assemblies 12 are bonded to the outer wall of the pressure maintaining cylinder 101 through heat-conducting silica gel, each thermal insulation assembly 12 includes a multi-stage semiconductor chilling plate 1207, an electric wire 1203 and an electrode holder 1201, the semiconductor chilling plate 1207 is clamped by a heat-conducting plate 1206 and a heat-radiating plate 1205, and heat-conducting silica gel is coated between contact surfaces; the cold end of the semiconductor refrigerating plate 1207 is connected with the outer wall of the pressure maintaining cylinder 101 through a heat conducting plate 1206, and the hot end of the semiconductor refrigerating plate is contacted with seawater through a radiating fin 1205; each stage of semiconductor refrigeration piece 1207 is respectively connected with each electrode holder 1201 through coupling nut 1204, and each electrode holder 1201 is established ties through electric wire 1203, and electric wire 1203 is connected with circuit tube 15 through watertight cable I1204.

As shown in fig. 12 and 13, the culture kettle 9 comprises a high-pressure cylinder 901, an end cover 902 and a sealing plug 903; the bottom opening of the high-pressure cylinder 901 is sealed by an end cover 902, a sealing plug 903 is arranged in the middle of the end cover 902, and the sealing plug 903 and the end cover 902 are sealed by a sealing ring VI 905; the culture kettle 9 is connected with the sampling cylinder 301 through a sealing plug 903, and an insertion cavity for inserting the top of the sampling rod 302 is arranged in the middle of the bottom of the sealing plug 903; a plurality of bolt blocking holes 904 are formed in the side wall of the bottom of the sealing plug 903, the bolt blocking holes 904 are arranged along the radial direction, a bolt blocking 905 is arranged in each bolt blocking hole 904, the front end of each bolt blocking hole 905 extends into the insertion cavity, and a bolt V907 is fixed on the rear side of each bolt blocking hole 905; the stop bolt 905 is connected with the end face opposite to the bolt V907 through a compression spring II 906, and the stop bolt 905 can move in the stop bolt hole 904 under the action of the compression spring II 906; the outer wall of the high-pressure cylinder 901 is provided with a high-pressure pipe connecting hole and is respectively connected with a pressure gauge 910 and an overflow valve 911 through a high-pressure pipe V.

As shown in fig. 14 and 15, the model of the main control chip of the controller is STC89C51, in fig. 15, P1.0 is used as a temperature signal inlet, P1.1 is used as a pressure signal inlet, P2.3 is used as a current increase output, and P2.4 is used as a current decrease output. The ports K1, K2 and K3 are used as the input of the keys SET, DOWN and UP, the pulse sending frequency can be manually controlled, and the pulse sending frequency is converted into corresponding pulse signals after being processed by the single chip microcomputer to control the working current of the semiconductor refrigerating sheet 1207, so that the refrigerating power is controlled, and the heat preservation effect is achieved. The actual temperature signal measured by the P1.0 is compared with the temperature signal given by the single chip microcomputer, and the working current of the semiconductor refrigerating chip 1207 is controlled and adjusted through the PID.

A pressure-maintaining, heat-preserving, sampling and transferring method for benthos comprises the following steps:

(1) before the benthos pressure-maintaining, heat-preserving, sampling and transferring system is launched, the lower cavity of the piston 402 in the pressure compensation device 4 is inflated with inert gas with the water depth and pressure of 0.3 time of the sampling point through the inflation valve 401, and at the moment, the piston 402 in the pressure compensation device 4 is positioned at the top of the cavity of the pressure compensation device 4; the heat preservation assembly 12 is arranged on the outer wall of the pressure preservation cylinder 101, is connected with a circuit, is connected with a power supply, and fixes the pressure preservation, heat preservation, sampling and transfer system of the benthos on the lander;

(2) opening the flap valve cover 202 and the switch valve 7 on the bait cylinder 6, fixing one end of a trigger rope II 801 on a trigger rod II 701, wherein one end of the trigger rod II is provided with a trigger rope mounting hole II 705, and the other end of the trigger rope II is bound on a bearing block of the lander; one end of a trigger rope I8 is fixed on one end of a trigger rod I501, a trigger rope mounting hole I502 is formed in one end of the trigger rod I, and the other end of the trigger rope I8 is bound on a bearing block of the lander;

(3) during the landing device lowering process, under the action of seawater pressure, the piston 402 of the pressure compensation device 4 moves downwards until the pressures in the lower cavity and the upper cavity of the piston 402 reach balance;

(4) when the pressure-maintaining, heat-preserving, sampling and transferring system for the benthos is put down to the designated seabed surface, the bait in the bait cylinder 6 flows into the pressure-maintaining cylinder 101 through the filter plate 602 to trap the benthos in the deep sea environment; when observing that benthos enters the pressure-maintaining cylinder 101, a camera on the lander releases the bearing block through the releaser, the trigger rod I501 and the trigger rod II 701 are triggered, the flap valve cover 202 and the switch valve 7 on the bait cylinder 6 are closed at the same time, and the lander floats upwards;

(5) in the process that the pressure maintaining, heat preserving and sampling and transferring system of the benthonic organism is recovered to the sea surface, the pressure sensor 13 sends a pressure signal due to the reduction of the pressure of the external seawater, the input end of the controller receives a real-time pressure signal from the pressure sensor 13, the pressure maintaining cylinder 101 expands and deforms, at the moment, the inert gas in the lower cavity of the piston 402 of the pressure compensating device 4 pushes the piston 402 to move to the upper cavity, the seawater in the upper cavity is forced to flow into the pressure maintaining cylinder 101 through the high-pressure pipe II 10, and therefore the pressure loss in the pressure maintaining cylinder 101 caused by the expansion and deformation of the pressure maintaining cylinder;

in the process that the benthos pressure-maintaining heat-preserving sampling transfer system is recycled to the sea surface, as the temperature of external seawater rises, the temperature sensor 14 sends a temperature signal, the input end of the controller receives a real-time temperature signal from the temperature sensor 14, the output end of the controller outputs a control signal, and the working current in the semiconductor refrigerating sheet 1207 is controlled to control the refrigerating power, the cold end of the semiconductor refrigerating sheet 1207 enables the temperature in the pressure-maintaining cylinder 101 to be always kept at the same temperature value as a sampling point through the heat conducting plate 1206, and the hot end transfers the heat to the seawater through the radiating fin 1205;

(6) after the pressure-maintaining, heat-preserving, sampling and transferring system for the benthos is recovered to the sea surface, the culture kettle 9 is butted with the pressure-maintaining, heat-preserving, sampling and transferring system for the benthos in a laboratory, and the pressure-maintaining, heat-preserving and transferring of the benthos can be carried out;

the specific operation of step (6) is as follows:

(6-1) before the culture kettle 9 is butted with the benthos pressure-maintaining, heat-preserving, sampling and transferring system, injecting seawater into the culture kettle 9, and enabling the pressure environment in the culture kettle 9 to be basically equal to the water depth pressure environment of the sampling point;

(6-2) in the process of butting the culture kettle 9 and the benthos pressure-maintaining, heat-preserving, sampling and transferring system, the stop bolt 905 is matched with the annular groove 311 at the upper end part of the sampling rod 302, so that the compression spring II 906 is compressed to realize butting, and then the culture kettle 9 is in sealing connection with the sampling device through the bolt V907;

(6-3) disconnecting the pressure sensor 13 and the temperature sensor 14 from the watertight cable II 16;

(6-4) connecting the pressure pump with the high-pressure valve 17, pressurizing the pressure maintaining cylinder 101 to a pressure which is 0.3MPa-0.7MPa higher than the water depth pressure of the sampling point, so that the sampling assembly 3 moves upwards, when the sampling assembly 3 moves to the circular hole II309 and the outlet of the pressure maintaining cylinder 101 is communicated, the pressurization is stopped, and the benthos in the sampling cylinder 3 moves to the culture kettle 9 from the circular hole I308 and the circular through hole 310, so that the pressure maintaining, heat preservation and transfer of the benthos are completed.

Claims

1. The utility model provides a benthos pressurize heat preservation sampling transfer system which characterized in that: the device comprises a pressure maintaining assembly for maintaining pressure, a sampling assembly for sampling benthos, a circuit cylinder for controlling, a temperature sensor for acquiring the temperature in the pressure maintaining assembly, a pressure sensor for acquiring the pressure in the pressure maintaining assembly and a culture kettle for transferring the benthos; the sampling assembly is inserted into the pressure maintaining assembly, the sampling assembly is connected with the culture kettle, an inlet sealing assembly is arranged at an inlet in the middle of the pressure maintaining assembly, a heat preservation assembly for heat preservation is bonded on the outer wall in the middle of the pressure maintaining assembly, a pressure compensation device for keeping the pressure balance in the pressure maintaining assembly and a bait tube for releasing bait are arranged on the outer wall at the bottom of the pressure maintaining assembly, the pressure compensation device is communicated with the pressure maintaining assembly, and the bait tube is communicated with the pressure maintaining assembly through a switch valve; a temperature sensor and a pressure sensor are arranged on the inner wall of the pressure maintaining assembly; the circuit barrel is installed on the outer wall of the pressure maintaining assembly, a power supply and a controller are arranged in the circuit barrel, the power supply supplies power to the heat insulating assembly, the controller, the temperature sensor and the pressure sensor, and the controller is connected with the temperature sensor, the pressure sensor and the heat insulating assembly respectively.

2. The benthonic organism pressure-maintaining, heat-preserving, sampling and transferring system according to claim 1, wherein: the pressure maintaining assembly comprises a pressure maintaining cylinder, a square stop bolt and a high-pressure valve I, wherein a plurality of square stop bolt holes are radially formed in the side wall of the top of the pressure maintaining cylinder, and a square stop bolt is arranged in each square stop bolt hole; keep off and tie the box through I fixed mounting of bolt corresponding to square fender bolt department on the pressurize section of thick bamboo outer wall, square fender bolt with keep off and tie between the relative terminal surface of box and be connected through compression spring I, square fender bolt can be in square fender bolt downthehole removal under compression spring I's effect, keep off to be equipped with on the bolt box and be used for carrying out spacing spacer pin to square fender bolt, keep off a section of thick bamboo inner chamber bottom and be connected with high-pressure valve I through high-pressure pipe I.

3. The pressure-maintaining, heat-preserving, sampling and transferring system for the benthos according to claim 2, wherein: the sampling assembly comprises a sampling cylinder, a sampling rod, an upper end cover and a lower end cover, wherein the upper end of the sampling cylinder is fixed on the lower end surface of the upper end cover, the lower end of the sampling cylinder is fixed on the upper end surface of the lower end cover, the sampling rod is fixed on the upper end surface of the upper end cover through a bolt II, and an annular groove is formed in the upper end part of the sampling rod; a sealing ring I is arranged on the side wall of the upper end cover, a through hole I is formed in the center of the lower end cover, and a sealing ring II is arranged on the side wall of the lower end cover; the upper part of the side wall of the sampling cylinder is provided with a circular hole I, the lower part of the side wall of the sampling cylinder is provided with a circular hole II, and the upper end part of the sampling cylinder is provided with a circular through hole; the diameter of the outlet of the pressure maintaining cylinder is the same as the diameter of the circular hole I, the circular hole II and the circular through hole.

4. The pressure-maintaining, heat-preserving, sampling and transferring system for the benthos according to claim 2, wherein: the inlet sealing assembly comprises a sealing cylinder, a flap valve seat, a flap valve cover, a flap shaft, a sealing ring III, a torsion spring and a sleeve; the flap valve seat is connected with the sealing cylinder inlet in a sealing way through a bolt III, a hinge lug is arranged on the flap valve seat, a flap shaft is fixed on the hinge lug, a flap valve cover is hinged with the flap valve seat through the flap shaft and the hinge lug to open and close the sealing cylinder inlet, the flap valve cover and the flap valve seat are sealed through a sealing ring III, and a torsion spring is also arranged on the flap shaft; the sleeve is arranged on the flap valve cover, and the sealing cylinder is provided with a trigger mechanism for limiting the sleeve and the flap valve cover; the trigger mechanism comprises a trigger rod I, one end of the trigger rod I is provided with a trigger rope mounting hole I and is connected with the trigger rope I through the trigger rope mounting hole I, the other end of the trigger rod I penetrates through the trigger rod mounting hole I on the side wall of the sealing cylinder and then is inserted into the sleeve, the trigger rod I and the trigger rod mounting hole I are sealed through a sealing ring IV, and the side wall of the sealing cylinder is provided with a limiting mechanism I for limiting the trigger rod I; the outlet of the sealing cylinder is communicated with the inlet in the middle of the pressure maintaining assembly 1; the sleeve on the flap valve cover and the central line of the trigger rod I on the trigger mechanism are on the same straight line.

5. The pressure-maintaining, heat-preserving, sampling and transferring system for the benthos according to claim 2, wherein: the pressure compensation device comprises a pressure-resistant cylinder, a piston, a compensator end cover and an inflation valve; the compensator end cover is hermetically arranged at an opening at the top of the pressure-resistant cylinder; the piston is arranged in the pressure-resistant cylinder; the compensator end cover is provided with a high-pressure pipe connecting hole and is communicated with the pressure maintaining cylinder through a high-pressure pipe II; the bottom of the pressure-resistant cylinder is provided with a through hole II which is connected with the inflation valve.

6. The pressure-maintaining, heat-preserving, sampling and transferring system for the benthos according to claim 2, wherein: the bait cylinder comprises a pressure-resistant cylinder body, a bait cylinder bottom cover and a filter plate; the bait cylinder bottom cover is arranged at the bottom of the pressure-resistant cylinder body through a bolt IV; bait is arranged in the pressure-resistant cylinder body; the top of the pressure-resistant cylinder body is provided with a filter plate, the filter plate is provided with filter holes, and the bait cylinder is communicated with the inner cavity of the pressure-maintaining cylinder through a high-pressure pipe III and a switch valve; the diameter of the filter hole on the filter plate is smaller than the inner diameter of the high-pressure pipe III.

7. The benthonic organism pressure-maintaining, heat-preserving, sampling and transferring system according to claim 6, wherein: the switch valve comprises an inlet port, an outlet port, a sealing ring V, a valve body and a trigger rod II; the lower portion of the valve body is provided with an inlet interface, the inlet interface is communicated with a bait barrel through a high-pressure pipe III, the upper portion of the valve body is provided with an outlet interface, the outlet interface is communicated with a pressure maintaining barrel through a high-pressure pipe IV, the middle portion of the valve body is provided with a cavity used for communicating the inlet interface with the outlet interface, one end of the cavity is provided with an opening, one end of the trigger rod II is provided with a trigger rope mounting hole II, the trigger rope II is connected with the trigger rope mounting hole II through the trigger rope mounting hole II, the other end of the trigger rod II is transversely inserted into the cavity from the opening of the cavity and can slide in the cavity, the opening of the cavity is provided with a limiting mechanism II used for limiting the trigger rod II, the size of the trigger rod II is matched with the size of the inner cavity, the trigger rod II.

8. The pressure-maintaining, heat-preserving, sampling and transferring system for the benthos according to claim 2, wherein: the heat preservation assemblies are divided into two groups, the heat preservation assemblies are bonded with the outer wall of the pressure maintaining cylinder through heat-conducting silica gel, each heat preservation assembly comprises a multistage semiconductor refrigeration piece, an electric wire and an electrode seat, the semiconductor refrigeration pieces are clamped by a heat-conducting plate and a heat-radiating fin, and the heat-conducting silica gel is coated between contact surfaces; the cold end of the semiconductor refrigeration piece is connected with the outer wall of the pressure maintaining cylinder through a heat conducting plate, and the hot end of the semiconductor refrigeration piece is contacted with the seawater through a radiating fin; each stage of semiconductor refrigeration piece is connected with each electrode holder through coupling nut respectively, establishes ties through the electric wire between each electrode holder, and the electric wire is connected with the circuit section of thick bamboo through watertight cable I.

9. The benthonic organism pressure-maintaining, heat-preserving, sampling and transferring system according to claim 1, wherein: the culture kettle comprises a high-pressure cylinder, an end cover and a sealing plug; the bottom opening of the high-pressure cylinder is sealed by an end cover, a sealing plug is arranged in the middle of the end cover, and the sealing plug and the end cover are sealed by a sealing ring VI; the culture kettle is connected with the sampling cylinder through a sealing plug, and an insertion cavity for inserting the top of the sampling rod is arranged in the middle of the bottom of the sealing plug; the side wall of the bottom of the sealing plug is provided with a plurality of stop bolt holes which are arranged along the radial direction, each stop bolt hole is internally provided with a stop bolt, the front end of each stop bolt extends into the insertion cavity, and the rear side of each stop bolt is fixed with a bolt V; the stop bolt is connected with the end face, opposite to the bolt V, of the bolt V through a compression spring II, and the stop bolt can move in the stop bolt hole under the action of the compression spring II.