CN211469141U - CCFC kit - Google Patents

Abstract

The invention relates to a newly developed CCFC kit, which is divided into two types, wherein the kit 1 is transported at normal temperature, and the kit 2 is transported by dry ice. The reagent boxes are sealed and comprise box bodies and openable box covers; the inside fixed plate that is equipped with of box body, set up hole and the recess that the reagent consumptive material was placed to the several on the fixed plate, the reagent consumptive material includes reagent bottle, syringe, centrifuging tube, freezes deposits the pipe, and above-mentioned reagent consumptive material is sealed, aseptic, and the reagent bottle is bottled to have the parting liquid, and syringe and centrifuging tube are empty, freezes to deposit the pipe and places mesenchymal stem cell, EGF, FGF and fibrinogen respectively. The kit is convenient to use, is suitable for transportation of cryopreserved cells and factors, is beneficial to maintaining the activity of the cells, avoids cross contamination, and is convenient for separation and recovery of the cells and composition of corresponding components of CCFC due to matched reagent consumables.

Description

CCFC kit

Technical Field

The utility model relates to a kit or reagent box, in particular to CCFC kit.

Background

According to statistics, osteoarthritis patients in China exceed 10% of the total population, and reach more than 1 hundred million people, the incidence rate increases with the increase of age, and the prevention and control situation is severe. At present, osteoarthritis is mainly treated by means of oral pain relieving medicine, chondroitin sulfate, articular cavity injection sodium hyaluronate, joint replacement and the like, and the problem of worn cartilage repair cannot be fundamentally solved. In recent years, rapidly developed regenerative medicine technology provides possibility for the repair treatment of osteoarthritis. The CCFC in-situ tissue regeneration Technology (also called Cell-factors-fibrin in-situ Collection treatment Technology), which is called Cell-Cytokines-fibrin Collection Technology in English, can effectively solve part of key technical problems in the regenerative medicine field of cartilage regeneration and the like, and has important application value in curing chronic osteoarthritis.

Until now, no matched kit is available on the market, only corresponding reagent consumables can be purchased, and the early reagent preparation and preparation work is relatively complex, the stem cell separation time is long, the primary growth is slow, and the treatment process is easy to be polluted; the factors in the composition relate to transgenic engineering strains.

CCFC kit has been equipped with corresponding consumptive material, reagents such as parting liquid, has solved the loaded down with trivial details of reagent consumptive material purchase and preliminary preparation work, makes the experimenter release from these heavy work in preliminary stage, and can use the reagent of optimizing to carry out work, and convenient to use is convenient.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a novel kit, convenient to use is applicable to the transportation of cytokine and the separation of cell and retrieves.

The utility model provides a CCFC kit is sealed, including box body and the lid that can open, the inside fixed plate that is equipped with of box body, set up the hole or the recess that the reagent consumptive material was placed to the several on the fixed plate. The reagent consumables include reagent bottle, centrifuging tube and cryopreserved pipe.

The 5ml syringes (5) are sealed and sterile, and the number of the syringes is 1.

The reagent bottles (6) are sealed and sterile, and contain 1 separating medium.

The 50ml centrifuge tubes (7) are sealed and sterile, and the number of the centrifuge tubes is 16.

The freezing tube (8-13) is sealed and sterile, and 3 mesenchymal stem cells, 1 EGF, 1 FGF and 1 fibrinogen are respectively placed in the freezing tube.

The reagent bottle (6) is a square aseptic bottle with a flat bottom; the 50ml centrifuge tube (7) is sterile; the freezing tube (8-13) is a flat-bottom sterile cylindrical bottle.

The box body and the box cover are connected or mutually independent, and the box cover is arranged on the box body to keep the reagent box in a sealed state.

The material of the reagent bottle 5ml injector (5), the reagent bottle (6), the 50ml centrifuge tube (7) and the freezing tube (8-13) is transparent plastic; all reagent consumables are placed in the holes or grooves respectively.

The reagent consumables are respectively placed in the holes or the grooves to play a role in fixing.

The CCFC kit is made of polypropylene plastic materials.

The kit 2 was shipped in dry ice.

The fixed plate is a sponge fixed plate or a foam fixed plate, and is detachably fixed inside the box body.

The utility model discloses a kit, convenient to use is applicable to the transportation of cytokine and the separation of cell and retrieves.

The kit is used for ultralow temperature transportation of CCFC stem cells and factors, extraction and recovery of the cells and combination and proportioning of the cells and the factors.

The inventor of the present application has extensively and deeply studied and designed a kit suitable for low temperature transportation of cells and factors, cell separation and recovery, and CCFC assembly, which is sealed and comprises a box body and an openable box cover, wherein a fixing plate is arranged in the box body, and a plurality of holes or grooves for placing reagent consumables and cytokines are arranged on the fixing plate. The reagent consumables are sealed and sterile. The kit is suitable for low-temperature transportation, cell separation and recovery and CCFC proportioning, can be directly used, is convenient and simple, and is beneficial to accelerating the production cycle. On this basis, the utility model discloses has been accomplished.

The utility model has the advantages that:

the kit is suitable for transporting the frozen cells and factors, can be directly used and shortens the production period.

Optimize corresponding reagent, be equipped with supporting consumptive material, can conveniently take, shorten loaded down with trivial details reagent in earlier stage and prepare, reduce stem cell's slow growth day.

Term(s) for

As used herein, "kit" may also be referred to as a kit, with the same meaning, all for the kit for the cryogenic transport of cells and factors, the cell separation recovery, and the CCFC combination.

Drawings

Fig. 1 is an appearance structure diagram of the reagent kit 1 of the present invention.

Fig. 2 is an appearance structure diagram of the reagent kit 2 of the present invention.

Fig. 3 is a schematic diagram of the internal structure of the kit 1 of the present invention.

Fig. 4 is a schematic diagram of the internal structure of the kit body of the kit 2 of the present invention.

1-box body; 2-a box cover; 3-a box body; 4-box cover; 5-5ml syringe; 6-reagent bottle; 7-50ml centrifuge tube; 8-13-freezing and storing the tube.

Detailed Description

The present invention will be further described with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The experimental procedures, in which specific conditions are not noted in the following examples, are generally carried out according to conventional conditions or according to conditions recommended by the manufacturers.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. In addition, any methods and materials similar or equivalent to those described herein can be used in the practice of the present invention. The preferred embodiments and materials described herein are intended to be exemplary only.

The reagent kit is divided into two types which are sealed and respectively comprise a box body (1, 3) and an openable box cover (2, 4). Kit 2 dry ice transport. The box body (1, 3) inside fixed plate that is equipped with, set up hole or the recess that the reagent consumptive material was placed to the several on the fixed plate, the reagent consumptive material includes 5ml syringe (5), reagent bottle (6), 50ml centrifuging tube (7), freezes deposits pipe (8-13).

The CCFC kit is characterized in that the 5ml syringes (5) are sealed and sterile, and the number of the syringes is 1.

The reagent bottles (6) are sealed and sterile, and contain 1 separating medium.

In the kit, the 5ml syringe (5) of the reagent bottle, the reagent bottle (6), the 50ml centrifuge tube (7) and the freezing tube (8-13) are made of transparent plastics; all reagent consumables are placed in the holes or grooves respectively.

The reagent bottle (6) is a square aseptic bottle with a flat bottom; the 50ml centrifuge tube (7) is sterile; the freezing tube (8-13) is a flat-bottom sterile cylindrical bottle.

The 50ml centrifuge tubes (7) are sealed and sterile, and the number of the centrifuge tubes is 16.

The freezing tube (8-13) is sealed and sterile, and 3 mesenchymal stem cells, 1 EGF, 1 FGF and 1 fibrinogen are respectively placed in the freezing tube.

The box bodies (1, 3) and the box covers (2, 4) are connected or mutually independent, and the box covers (2, 4) are arranged on the box bodies (1, 3) to keep the reagent box in a sealed state.

The CCFC kit is made of polypropylene plastic materials.

The CCFC kit and the kit 2 are placed in dry ice for transportation.

The CCFC kit, the fixed plate is a sponge fixed plate or a foam fixed plate, and the fixed plate is detachably fixed in the box bodies (1, 3).

The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.

Claims (10)

1. A CCFC kit is characterized in that the kit is divided into two types, both are sealed and both comprise a box body (1, 3) and an openable box cover (2, 4); transporting the kit 1 at normal temperature, and transporting the kit 2 by dry ice; the box body (1, 3) inside fixed plate that is equipped with, set up hole or the recess that the reagent consumptive material was placed to the several on the fixed plate, the reagent consumptive material includes 5ml syringe (5), reagent bottle (6), 50ml centrifuging tube (7), freezes deposits pipe (8-13).

2. The CCFC kit according to claim 1, wherein the 5ml syringes (5) are sealed and sterile, the number of the syringes is 1, and the 5ml syringes (5), the reagent bottles (6), the 50ml centrifuge tubes (7) and the freezing tubes (8-13) are made of transparent plastics; all reagent consumables are placed in the holes or grooves respectively.

3. CCFC kit according to claim 1, characterized in that the reagent bottles (6) are sealed, sterile, containing 1 separation liquid.

4. CCFC kit according to claim 1, characterized in that the 50ml centrifuge tubes (7) are sealed, sterile and 16 in number.

5. The CCFC kit according to claim 1, wherein the cryopreservation tubes (8-13) are sealed and sterile, and hold 3 mesenchymal stem cells, 1 EGF, 1 FGF and 1 fibrinogen, respectively.

6. CCFC kit according to claim 1, characterized in that the reagent bottles (6) are flat-bottomed square aseptic bottles; the 50ml centrifuge tube (7) is sterile; the freezing tube (8-13) is a flat-bottom sterile cylindrical bottle.

7. CCFC kit according to claim 1, characterized in that the case bodies (1, 3) and the case covers (2, 4) are connected or independent of each other, the case covers (2, 4) being on the case bodies (1, 3) to keep the kit in a sealed state.

8. A CCFC kit according to any one of claims 1 to 3, wherein the materials are all polypropylene plastics.

9. The CCFC kit according to claim 1, wherein kit 2 is shipped in dry ice.

10. CCFC kit according to claim 1, characterised in that the holder is a sponge holder or a foam holder, removably fixed inside the case (1, 3).

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