CN211453081U - Portable immunofluorescence staining device for oocytes and embryos - Google Patents
Portable immunofluorescence staining device for oocytes and embryos Download PDFInfo
- Publication number
- CN211453081U CN211453081U CN202020160892.2U CN202020160892U CN211453081U CN 211453081 U CN211453081 U CN 211453081U CN 202020160892 U CN202020160892 U CN 202020160892U CN 211453081 U CN211453081 U CN 211453081U
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- China
- Prior art keywords
- sample
- liquid
- groove
- sample bottle
- box body
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- Expired - Fee Related
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- 210000000287 oocyte Anatomy 0.000 title claims abstract description 22
- 238000003125 immunofluorescent labeling Methods 0.000 title claims abstract description 18
- 210000002257 embryonic structure Anatomy 0.000 title claims description 14
- 239000007788 liquid Substances 0.000 claims abstract description 76
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 35
- 239000010865 sewage Substances 0.000 claims description 17
- 238000007599 discharging Methods 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 8
- 230000006835 compression Effects 0.000 claims description 4
- 238000007906 compression Methods 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- 210000001161 mammalian embryo Anatomy 0.000 abstract description 5
- 230000000903 blocking effect Effects 0.000 abstract description 2
- 238000004140 cleaning Methods 0.000 description 7
- 239000012530 fluid Substances 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 238000010186 staining Methods 0.000 description 3
- 229930040373 Paraformaldehyde Natural products 0.000 description 2
- 238000012744 immunostaining Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 229920002866 paraformaldehyde Polymers 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000000739 chaotic effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model discloses a portable immunofluorescence staining apparatus for oocyte and embryo, it includes the box body, and the upper end intermediate position of box body sets up a plurality of sample grooves side by side, and the both sides that are located the sample groove set up a plurality of reagent grooves, and the bottom in every reagent groove all communicates with the sample groove through the pipeline, sets up a plurality of liquid outlets on the inside wall in every sample groove. The upper end of each reagent groove is in threaded connection with a cover body. A sample bottle can be rotatably arranged in each sample groove, a liquid inlet with the same size as the liquid outlet is formed in the side wall of each sample bottle, and a blocking mechanism for opening and closing the liquid inlet is arranged on the side wall of each sample bottle. The inside wall of sample bottle is provided with the liquid level scale mark. The diapire skew axle center position of sample bottle is provided with blowdown hole I, and what correspond sets up blowdown hole II on the diapire of box body, and rotatory blowdown hole I and the blowdown hole II that arrive certain angle lie in the same axis. The utility model has the characteristics of get liquid, annotate liquid and wash in an organic whole.
Description
Technical Field
The utility model relates to a portable immunofluorescence staining device for oocyte and embryo.
Background
The immunofluorescence technique is a technique established on the basis of immunology, biochemistry and microscope techniques, and utilizes the principle of antigen-antibody reaction to determine the nature and location of antigen or antibody. When the immunostaining test is carried out, a plurality of test steps of cell preparation, fixation, permeability, sealing, primary antibody combination, secondary antibody combination and mounting detection are required, a plurality of reagents are required in the test process, and each reagent is easily disordered and extremely inconvenient to take when being placed and alternately used. When taking reagent and injecting into the sample bottle, need with the help of other instruments, lead to the process loaded down with trivial details, influence work efficiency. Therefore, the portable immunofluorescence staining device for oocytes and embryos, which integrates liquid taking, liquid injection and cleaning, is designed to have wide market prospect.
SUMMERY OF THE UTILITY MODEL
When putting and using by turns to foretell every kind of reagent, appear chaotic easily, it is also extremely inconvenient to take, when taking reagent and pouring into the sample bottle into, need with the help of other instruments, lead to the process loaded down with trivial details, influence prior art such as work efficiency not enough, the utility model provides a get liquid, annotate liquid and wash a portable immunofluorescence staining device for oocyte and embryo in an organic whole.
The utility model provides a technical scheme that its technical problem adopted is:
a portable immunofluorescence staining device for oocytes and embryos comprises a box body, wherein a plurality of sample grooves are arranged in the middle of the upper end of the box body side by side, a plurality of reagent grooves are arranged on two sides of each sample groove, the bottom end of each reagent groove is communicated with the sample grooves through a pipeline, and a plurality of liquid outlets are formed in the inner side wall of each sample groove; the upper end of each reagent groove is in threaded connection with a cover body; a sample bottle is rotatably arranged in each sample groove, a liquid inlet with the same size as the liquid outlet is formed in the side wall of each sample bottle, a plugging mechanism for opening and closing the liquid inlet is arranged on the side wall of each sample bottle, the plugging mechanism comprises a vertical open groove formed in the side wall of each sample bottle and a separation baffle plate sliding on the vertical open groove, a through hole with the same size as the liquid inlet is formed in the separation baffle plate, and a compression spring is arranged in the vertical open groove; the inner side wall of the sample bottle is provided with liquid level scale marks; the bottom wall of the sample bottle is provided with a sewage discharging hole I in the position deviated from the axis, a sewage discharging hole II is correspondingly arranged on the bottom wall of the box body, and the sewage discharging hole I rotating to a certain angle and the sewage discharging hole II are located on the same axis.
Further, the inside of box body is provided with the cavity that is used for holding heat preservation liquid, the upper and lower side of cavity is provided with entry and export respectively, all be provided with the valve on entry and the export.
Furthermore, the diameter of the sewage discharge hole II is larger than that of the sewage discharge hole I, and a filter screen with the aperture of 100 mu m is arranged in the sewage discharge hole I.
Furthermore, each liquid outlet is provided with a sealing ring.
Furthermore, a strip-shaped settling tank is arranged in the middle of the box body, and the reagent tank is arranged on the bottom wall of the settling tank.
Further, the inner bottom wall of the sample bottle is arranged from the edge to the sewage discharge hole I in an inclined and downward mode.
Furthermore, a plurality of sign lines are arranged on the upper surface of the box body in an arc shape, and each sign line corresponds to the liquid outlet one to one.
The utility model has the advantages that:
by arranging a plurality of sample grooves, immunofluorescence staining experiments can be carried out on a plurality of oocytes or embryos. Through passing through pipeline and a plurality of sample cell intercommunication in the low side in every reagent groove, can inject different reagents into the sample cell, annotate liquid convenient and fast and be difficult to appear the confusion. By arranging the plugging mechanism, the liquid inlet can be opened and closed at any time, and the operation is simple and convenient. Through setting up blowoff hole I and blowoff hole II, when both axes align, liquid flows out. Through setting up the cavity, can keep warm to the box body. The depth of the sample tank can be reduced by arranging the settling tank, so that the observation is facilitated. The volume of liquid flowing into the reagent tank can be read by setting liquid level scale marks. By setting the marker line, when the partition plate is aligned with the marker line, the partition plate is pressed down, and the liquid flows from the reagent tank into the sample tank.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a schematic sectional view of the present invention;
fig. 3 is an enlarged schematic structural view of a part a in fig. 2 of the present invention;
in the figure, a box body 1, a sample groove 11, a liquid outlet 111, a sealing ring 112, a reagent groove 12, a pipeline 121, a cover body 122, a strip-shaped groove 122a, a sewage discharge hole II 13, a cavity 14, a settling tank 15, a mark line 16, a supporting leg 17, a sample bottle 2, a liquid inlet 21, a liquid level scale line 22, a sewage discharge hole I23, a blocking mechanism 3, a vertical open groove 31, a baffle 32, a through hole 321, a spherical head 322 and a compression spring 33.
Detailed Description
For a better understanding of the present invention, embodiments thereof are explained in detail below with reference to the accompanying drawings.
As shown in the attached drawings 1 to 3, the portable immunofluorescence staining device for oocytes and embryos comprises a box body 1, wherein the bottom of the box body 1 is fixedly connected with four supporting legs 17, a plurality of sample grooves 11 are arranged in parallel at the middle position of the upper end of the box body 1, the depth of each sample groove 11 is 3cm, and the diameter of each sample groove 11 is 1.5 cm. A plurality of reagent grooves 12 are arranged on two sides of the sample groove 11, the depth of each reagent groove 12 is 10cm, the diameter of each reagent groove 12 is 3cm, and a large amount of reagents can be contained in the reagent grooves 12. In FIG. 1, four sample wells 11 and eight reagent wells 12 are shown, allowing immunofluorescent staining of a plurality of oocytes and embryos. The eight reagent tanks 12 are used for containing PBS, paraformaldehyde, cleaning fluid, membrane permeation fluid, sealing fluid, primary antibody fluid, secondary antibody fluid and staining fluid respectively.
The bottom end of 12 of each reagent groove is communicated with the sample groove 11 through a pipeline 121, in order to increase the inclination degree of the pipeline 121, a strip-shaped settling tank 15 is arranged in the middle of the box body 1, and the reagent grooves 12 are arranged on the bottom wall of the settling tank 15. A plurality of liquid outlets 111 are arranged on the inner side wall of each sample groove 11, the liquid outlets 111 are positioned above the bottom wall of the sample groove 11, and when a dyeing experiment is carried out, the liquid amount injected into the sample groove 11 reaches the position of 1.5cm deep. The upper end of each reagent well 12 is screwed to a lid 122. for better illustration, the lid 122 is shown only on the left reagent well 12. A strip-shaped groove 122a is arranged above the cover body 122, and when in use, the cover body 122 is rotated through the strip-shaped groove 122 a. During the experiment, the cover 122 is opened to prevent the negative pressure from being generated in the reagent tank 12, and after the experiment is completed, the cover 122 is closed to prevent the liquid from flowing out. Each sample cell 11 is rotatably provided with a sample bottle 2, and the side wall of the sample bottle 2 is provided with a liquid inlet 21 with the same size as the liquid outlet 111.
The side wall of the sample bottle 2 is provided with a plugging mechanism 3 for opening and closing the liquid inlet 21, the plugging mechanism 3 comprises a vertical opening groove 31 formed in the side wall of the sample bottle 2 and a baffle 32 sliding on the vertical opening groove 31, a through hole 321 with the same size as the liquid inlet 21 is formed in the baffle 32, and when the liquid outlet 111, the through hole 321 and the liquid inlet 21 are aligned, the liquid in the reagent tank 12 flows into the sample bottle 2 by pressing the baffle 32. Preferably, as shown in fig. 2, a ball head 322 is fixed to the top end of each baffle 32 to press the baffle 32. A compression spring 33 is arranged in the vertical opening groove 31 to realize the automatic reset of the baffle 32. The inner side wall of the sample bottle 2 is provided with a liquid level scale 22, and the volume of the injected liquid can be observed through the liquid level scale 22. In order to prevent liquid from flowing into the gap between the sample well 11 and the sample bottle 2, a sealing ring 112 is provided on each liquid outlet 111. Each liquid outlet 111 is communicated with the liquid in the corresponding reagent tank 12, and the liquid needed in the experiment is directly selected to rotate the sample bottle 2 pair to align the liquid inlet 21 with the corresponding liquid outlet 111.
The upper surface of the box body 1 is provided with a plurality of mark lines 16 in an arc shape, each mark line 16 corresponds to the liquid outlet 111 one by one, and the alignment of the liquid outlet 111 and the liquid inlet 21 can be ensured through the mark lines 16.
The diapire of sample bottle 2 deviates from the axle center position and is provided with blowdown hole I23, and the inside diapire of sample bottle 2 is by the edge to blowdown hole I23 slope downward setting. Set up blowdown hole II 13 on the diapire of box body 1 correspondingly, rotatory blowdown hole I23 and the blowdown hole II 13 of certain angle are located the same axis. The diameter of the drain hole II 13 is larger than that of the drain hole I23, so that liquid in the sample groove 11 can be conveniently discharged. The diameter of the oocyte is about 150 μm, and in order to prevent the oocyte from flowing out of the drain hole I23, a filter having a pore size of 100 μm is provided in the drain hole I23, so that the fluid flows out of the drain hole I23 and the oocyte remains in the sample bottle 2.
The inside of box body 1 is provided with the cavity 14 that is used for holding heat preservation liquid, and the upper and lower both ends of cavity 14 are provided with entry 141 and export 142 respectively, all are provided with valve 143 on entry 141 and the export 142, and during the use, pour the heat preservation liquid into from entry 141, use the back of accomplishing from export 142.
When the oocyte or the embryo cell is subjected to immunofluorescence staining, the injection sequence of the liquid is PBS, paraformaldehyde, cleaning liquid, membrane permeation liquid, sealing liquid, cleaning liquid, primary antibody liquid, cleaning liquid, secondary antibody liquid, cleaning liquid, staining liquid and cleaning liquid, and the aim of immunostaining is finally fulfilled through cell preparation, fixation, permeation, sealing, primary antibody combination, secondary antibody combination and staining. After the experiment is finished, the oocyte or embryo is taken out for mounting and fluorescent microscopic examination.
Claims (7)
1. A portable immunofluorescence staining apparatus for oocytes and embryos, characterized in that: the device comprises a box body, wherein a plurality of sample grooves are arranged in the middle of the upper end of the box body side by side, a plurality of reagent grooves are arranged on two sides of each sample groove, the bottom end of each reagent groove is communicated with the sample grooves through a pipeline, and a plurality of liquid outlets are arranged on the inner side wall of each sample groove; the upper end of each reagent groove is in threaded connection with a cover body; a sample bottle is rotatably arranged in each sample groove, a liquid inlet with the same size as the liquid outlet is formed in the side wall of each sample bottle, a plugging mechanism for opening and closing the liquid inlet is arranged on the side wall of each sample bottle, the plugging mechanism comprises a vertical open groove formed in the side wall of each sample bottle and a separation baffle plate sliding on the vertical open groove, a through hole with the same size as the liquid inlet is formed in the separation baffle plate, and a compression spring is arranged in the vertical open groove; the inner side wall of the sample bottle is provided with liquid level scale marks; the bottom wall of the sample bottle is provided with a sewage discharging hole I in the position deviated from the axis, a sewage discharging hole II is correspondingly arranged on the bottom wall of the box body, and the sewage discharging hole I rotating to a certain angle and the sewage discharging hole II are located on the same axis.
2. A portable immunofluorescent staining apparatus for oocytes and embryos as claimed in claim 1 wherein: the inside of box body is provided with the cavity that is used for holding heat preservation liquid, the upper and lower side of cavity is provided with entry and export respectively, all be provided with the valve on entry and the export.
3. A portable immunofluorescent staining apparatus for oocytes and embryos as claimed in claim 1 wherein: the diameter of the sewage discharge hole II is larger than that of the sewage discharge hole I, and a filter screen with the aperture of 100 mu m is arranged in the sewage discharge hole I.
4. A portable immunofluorescent staining apparatus for oocytes and embryos as claimed in claim 1 wherein: and each liquid outlet is provided with a sealing ring.
5. A portable immunofluorescent staining apparatus for oocytes and embryos as claimed in claim 1 wherein: the middle of the box body is provided with a strip-shaped settling tank, and the reagent tank is arranged on the bottom wall of the settling tank.
6. A portable immunofluorescent staining apparatus for oocytes and embryos as claimed in claim 1 wherein: the inner side bottom wall of the sample bottle is arranged from the edge to the drainage hole I in an inclined and downward mode.
7. A portable immunofluorescent staining apparatus for oocytes and embryos as claimed in claim 1 wherein: the upper surface of box body is the arc and sets up a plurality of mark lines, every the mark line with the liquid outlet one-to-one.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020160892.2U CN211453081U (en) | 2020-02-11 | 2020-02-11 | Portable immunofluorescence staining device for oocytes and embryos |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020160892.2U CN211453081U (en) | 2020-02-11 | 2020-02-11 | Portable immunofluorescence staining device for oocytes and embryos |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211453081U true CN211453081U (en) | 2020-09-08 |
Family
ID=72297909
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020160892.2U Expired - Fee Related CN211453081U (en) | 2020-02-11 | 2020-02-11 | Portable immunofluorescence staining device for oocytes and embryos |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211453081U (en) |
-
2020
- 2020-02-11 CN CN202020160892.2U patent/CN211453081U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200908 |
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| CF01 | Termination of patent right due to non-payment of annual fee |