CN211428117U - Capillary electrophoresis mass spectrometry combined equipment and biochemical analysis system - Google Patents
Capillary electrophoresis mass spectrometry combined equipment and biochemical analysis system Download PDFInfo
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- CN211428117U CN211428117U CN202020298018.5U CN202020298018U CN211428117U CN 211428117 U CN211428117 U CN 211428117U CN 202020298018 U CN202020298018 U CN 202020298018U CN 211428117 U CN211428117 U CN 211428117U
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Abstract
The utility model discloses a capillary electrophoresis mass spectrometry combination equipment and biochemical analysis system, capillary electrophoresis mass spectrometry combination equipment connect between electrophoresis apparatus and mass spectrograph, include: the electrospray ionization source and the clamping mechanism are arranged in the outer shell. Wherein, the outer shell has an inner cavity isolated from the outside; the electrospray ion source is provided with an electrophoresis connecting end, a chemical modification liquid injection end and an electrospray output end, wherein the electrophoresis connecting end is communicated with the electrophoresis apparatus, and the chemical modification liquid injection end is communicated with the chemical modification liquid; the electrospray ion source is suitable for being clamped in the clamping mechanism, the clamping mechanism is provided with a limiting installation position, the electrospray output end is limited and installed in the limiting installation position, and the limiting installation position is right opposite to the ion inlet of the mass spectrometer. Due to the arrangement of the closed inner cavity, disturbance of the external world to the Taylor cone of the electrospray ion source is reduced, and the accuracy of a mass spectrum detection signal is improved; the direct installation of the electrospray ion source is realized through a clamping mechanism.
Description
Technical Field
The utility model relates to a biochemical analysis device technical field, concretely relates to capillary electrophoresis mass spectrometry combination equipment and biochemical analysis system.
Background
The qualitative and quantitative analysis of the scientifically significant analytes in the complex samples is of great significance to modern medicine, biology, food medicine and environmental science. Mass Spectrometry (MS) is one of the most powerful qualitative and quantitative detection techniques for organic analytes, and relies on mass-to-charge ratio (m/z) detection to identify analytes. The mass spectrum technology has extremely high selectivity, higher sensitivity and rich tandem mass spectrum information collection capability. In modern analytical chemistry, a separation-detection two-dimensional biochemical analysis platform is constructed by combining a mass spectrometry detection technology after complex samples are separated, and technologies for achieving the purpose of qualitative and quantitative analysis include High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Supercritical Fluid Chromatography (SFC), Capillary Electrophoresis (CE), and the like. Capillary electrophoresis achieves separation of different components depending on the charge and hydrodynamic diameter of the analyte in an electrophoretic background electrolyte solution (BGE). Compared with the most common high performance liquid chromatography, the capillary electrophoresis technology has the advantages of simple operation, extremely low sample consumption and solution consumption, high sample separation efficiency, high analysis speed and strong separation capability aiming at biological macromolecules.
In the prior art, in order to realize online coupling of capillary electrophoresis and mass spectrometry detection equipment, a capillary electrophoresis mass spectrometry interface (CE-MS interface) is usually used to connect an electrophoresis apparatus and a mass spectrometer. The main element of the capillary electrophoresis mass spectrometry interface is an electrospray ion source, and patent document WO2009109037A1 discloses a stainless steel electrospray ion source which comprises a stainless steel three-way pipeline, wherein the three-way pipeline is respectively connected with a capillary electrophoresis separation capillary, a stainless steel electrospray spray needle and a chemical modification liquid pipeline. When the analyte is injected into the separation capillary filled with the background electrolyte solution, the analyte is separated under the driving of an electric field, and the separation capillary is connected with one of the three-way pipelines, so that the separated electrolyte solution containing the analyte is injected into the electrospray ion source, and the mass spectrum detection is realized. The chemical modification liquid is provided by an injection pump and is connected with one of the three-way pipelines. Electrospray high voltage is provided and controlled by the mass spectrometer to achieve an electrospray ionization process.
When the conventional ion source is connected with a mass spectrometer and an electrophoresis apparatus for biochemical analysis, the electrospray ion source needs to be installed and fixed by combining an iron support and a plastic fixing part, but the mass spectrometer belongs to a detection apparatus with high sensitivity, and is easy to be influenced by external temperature, airflow and vibration, so that the accuracy of a detection result is reduced, and the biochemical analysis result is influenced; in addition, in order to ensure that the electrospray ion source is just opposite to an ion inlet of the mass spectrometer, the iron support and the plastic fixing piece are constructed with the mass spectrometer, and operation errors are easy to generate, so that the position of the ion source needs to be debugged for many times, however, along with the increase of debugging times, the connection between the electrospray ion source and the plastic fixing piece is easy to loosen, the actual installation position of the electrospray ion source relative to the iron support is easy to move, the installation stability of the electrospray ion source is reduced, and errors occur in an analysis result.
SUMMERY OF THE UTILITY MODEL
Therefore, the technical problem to be solved by the present invention is that in the prior art, when the ion source and the mass spectrometer are connected and used on line, the electrospray ion source is installed by directly fixing the electrospray ion source on the iron stand through the plastic fixing member, and is easily affected by external temperature, air flow and vibration, which causes the reduction of the accuracy of the mass spectrum detection signal; and after the electrospray ion source is disassembled and assembled for many times, the installation stability is reduced, and the analysis result has errors.
Therefore, the utility model provides a capillary electrophoresis mass spectrometry combination device which is connected between an electrophoresis apparatus and a mass spectrometer; it is characterized by comprising:
the outer shell is provided with an inner cavity isolated from the outside;
the electrospray ionization source is provided with an electrophoresis connecting end, a chemical modification liquid injection end and an electrospray output end, the electrophoresis connecting end is communicated with the electrophoresis apparatus, and the chemical modification liquid injection end is communicated with the chemical modification liquid;
the electrospray ion source is suitable for being clamped in the clamping mechanism, the clamping mechanism is provided with a limiting installation position, an electrospray output end is installed in the limiting installation position in a limiting mode, and the limiting installation position is right opposite to an ion inlet of a mass spectrometer.
Optionally, in the apparatus for capillary electrophoresis mass spectrometry, the clamping mechanism includes: the supporting part is used for supporting the electrospray ion source;
and the cover arranging part is buckled with the bearing part, an accommodating cavity for limiting and installing the electrospray ion source is formed on the bearing part and the cover arranging part, and the limiting and installing position is arranged in the accommodating cavity.
Optionally, in the above apparatus for capillary electrophoresis mass spectrometry, the accommodating chamber further has a first auxiliary mounting position fitted to the electrophoresis connecting end and a second auxiliary mounting position fitted to the chemical modification solution injection end;
the first auxiliary mounting position, the second auxiliary mounting position and the limiting mounting position are limiting grooves formed in the bearing part.
Optionally, in the above-mentioned capillary electrophoresis mass spectrometry apparatus, the clamping mechanism further includes at least one top-extension limiting member, and one end of the top-extension limiting member is installed at the bottom of the limiting groove and extends toward one side of the covering portion; the other end of the top extension limiting part is abutted against the end face of one side, facing the bottom of the tank, of the electrospray ion source.
Optionally, in the aforementioned capillary electrophoresis mass spectrometry apparatus, the clamping mechanism further includes at least one locking member, and the locking member is installed between the cover setting portion and the support portion, and is used to lock the closing between the cover setting portion and the support portion.
Optionally, the apparatus for capillary electrophoresis mass spectrometry further comprises a position adjusting mechanism connected to the clamping mechanism,
the clamping mechanism comprises an adjusting driving end and an adjusting connecting end, wherein the adjusting driving end is arranged on the outer side of the outer shell, the adjusting connecting end is connected with the clamping mechanism, and the adjusting driving end drives the adjusting connecting end to move in the inner cavity.
Optionally, the apparatus for capillary electrophoresis mass spectrometry further comprises a probe cleaning mechanism,
the cleaning device comprises a connecting pipe extending into the inner cavity, wherein one end of the connecting pipe is connected with a cleaning air source, the other end of the connecting pipe is provided with a spraying hole close to the electric spray output end, and the spraying hole is used for releasing cleaning air towards the electric spray output end.
Optionally, the capillary electrophoresis mass spectrometry apparatus further includes an image collecting device having a processor and a camera for collecting an image of the electrospray output end, the camera is electrically connected to the processor, and the collected image is output to the processor;
the display device is arranged on the outer shell and electrically connected with the image acquisition device, and the display device is used for displaying the image acquired by the camera.
Optionally, the capillary electrophoresis mass spectrometry apparatus further includes a light source disposed in the inner cavity to improve brightness of an output image at an output end of the electrospray ionization.
Optionally, in the above capillary electrophoresis mass spectrometry apparatus, the outer casing includes an upper cover body and a casing body, and the upper cover body is arranged on the casing body in a foldable manner.
A biochemical analysis system, comprising:
an electrophoresis apparatus and a mass spectrometer;
the device is characterized by further comprising the capillary electrophoresis mass spectrometry combination equipment, wherein the capillary electrophoresis mass spectrometry combination equipment is connected between the electrophoresis apparatus and the mass spectrometer.
The technical scheme provided by the utility model, following advantage has:
1. the utility model provides a capillary electrophoresis mass spectrometry combination equipment connects between electrophoresis apparatus and mass spectrograph, include: the electrospray ionization source and the clamping mechanism are arranged in the outer shell. Wherein, the outer shell has an inner cavity isolated from the outside; the electrospray ion source is provided with an electrophoresis connecting end, a chemical modification liquid injection end and an electrospray output end, wherein the electrophoresis connecting end is communicated with an electrophoresis apparatus, and the chemical modification liquid injection end is communicated with a chemical modification liquid; the electrospray ion source is suitable for being clamped in the clamping mechanism, the clamping mechanism is provided with a limiting installation position, the electrospray output end is installed in the limiting installation position in a limiting mode, and the limiting installation position is right opposite to an ion inlet of a mass spectrometer.
According to the capillary electrophoresis mass spectrometry combined equipment with the structure, the disturbance of the outside on the Taylor cone of the electrospray ion source is reduced through the arrangement of the closed inner cavity, and the accuracy of a mass spectrometry detection signal is improved; meanwhile, in the clamping mechanism, the arrangement of the limiting installation positions can further ensure that the electrospray ions are correspondingly fixed to the mass spectrometer, further reduce the influence of the outside on the electrospray ion source, greatly improve the installation stability of the electrospray ion source and avoid the error of an analysis result in the use process of the ion source; meanwhile, when the capillary electrophoresis mass spectrometry combination equipment is used, the electrospray ion source is directly installed through the clamping mechanism, and the continuous disassembly of the electrospray ion source from the limiting installation position is avoided, so that the installation reliability between the limiting installation position and the electrospray output end is ensured, and the occurrence of analysis result errors is avoided.
2. The utility model provides a capillary electrophoresis mass spectrometry combination equipment, fixture includes: a support part and a covering part. The supporting part is used for supporting the electrospray ion source; the cover establishing part and the bearing part are buckled with each other, a containing cavity for limiting and installing the electrospray ion source is formed on the bearing part and the cover establishing part, and the limiting and installing position is arranged in the containing cavity. The capillary electrophoresis mass spectrometry combination equipment with the structure is provided with the bearing part and the cover part, so that the bearing part and the cover part are buckled with each other to realize the containing cavity, and the containing cavity further ensures the stability of fixing the electrospray ion source.
3. The utility model provides a capillary electrophoresis mass spectrometry equipment, the holding cavity is also provided with a first auxiliary mounting position matched with the electrophoresis connecting end and a second auxiliary mounting position matched with the chemical modification liquid injection end; the first auxiliary mounting position, the second auxiliary mounting position and the limiting mounting position are limiting grooves formed in the bearing part. The electric spray ion source is limited by the limiting groove, and the first auxiliary mounting position and the second auxiliary mounting position further fix the electric spray ion source, so that the stability of the electric spray ion source is improved.
4. The utility model provides a capillary electrophoresis mass spectrometry equipment, the fixture still includes at least one top and stretches the locating part, the one end of top stretch locating part is installed at the tank bottom of spacing recess, and extend towards one side direction of the portion of covering; the other end of the top extension limiting part is abutted against the end face of one side, facing the bottom of the tank, of the electrospray ion source.
The arrangement of the top extension limiting part ensures that the electrospray ion source is stably clamped between the top extension limiting part and the cover arrangement part, and further ensures the installation stability of the electrospray ion source; and the top extension limiting part is electrically connected with a contact formed by the electrospray ion source, so that the conductive stability is realized.
5. The utility model provides a capillary electrophoresis mass spectrometry combination equipment still includes position control mechanism, with fixture connects the setting, be in including setting up the regulation drive end in the shell body outside, and with fixture connects the regulation link that sets up, adjust the drive end drive it is in to adjust the link remove in the inner chamber. And then the position of an electrospray output end of the electrospray ion source to an ion inlet of a mass spectrometer is adjusted in multiple dimensions, so that the stability and the sensitivity of the electrospray ionization process are ensured.
6. The utility model provides a capillary electrophoresis mass spectrometry equipment still includes the clean mechanism of probe, it including stretch into in the connecting pipe of inner chamber, the one end and the clean air supply of connecting pipe are connected, and the other end has and is close to the electric spray output sets up the blowout port, the blowout port orientation the clean gas of electric spray output release cleans the electric spray output automatically, need not to open the shell body to can not introduce any possible ion source pollution, guaranteed capillary electrophoresis mass spectrometry equipment's cleanliness.
7. The utility model provides a capillary electrophoresis mass spectrometry equipment, still include the image acquisition device, have the treater and gather the camera of the image of electricity spraying output end, the camera with the treater electricity is connected, gather the image output to the treater;
the camera comprises a shell body, and is characterized by further comprising a display device arranged on the shell body and electrically connected with the image acquisition device, wherein the display device is used for displaying images acquired by the camera side. The setting of image acquisition device, the user of being convenient for observes actual conditions in real time in the use, and simultaneously, display device's setting further improves the intuition nature of surveing.
8. The utility model provides a capillary electrophoresis mass spectrometry combination equipment is still including setting up the light source body in the inner chamber, in order to improve the luminance and the definition after the image amplification of assurance collection of the luminance of electric spray output one side.
9. The utility model provides a capillary electrophoresis mass spectrometry combination equipment, the shell body includes lid and casing body, the lid can turn over to roll over ground and set up on the casing body. Guarantee when in-service use operation, go up the lid and can open by one side, and then install the electrospray ion source, change convenient operation, equipment ease for use is high.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the technical solutions in the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a capillary electrophoresis mass spectrometry apparatus provided in example 1;
fig. 2 is a schematic structural diagram of the capillary electrophoresis mass spectrometry apparatus provided in example 1 when the upper cover is opened;
fig. 3 is a schematic structural diagram illustrating that the upper cover and the covering portion of the apparatus for capillary electrophoresis mass spectrometry provided in example 1 are simultaneously opened;
fig. 4 is a schematic structural diagram of the clamping mechanism when the cover of the capillary electrophoresis mass spectrometry apparatus provided in embodiment 1 is opened;
FIG. 5 is an exploded view of an image acquisition structure of the apparatus for capillary electrophoresis mass spectrometry provided in FIG. 1;
FIG. 6 is a schematic view of a structure of a capillary electrophoresis mass spectrometry apparatus provided in example 1, with a viewing angle on a surface facing a mass spectrometer;
FIG. 7 is a sectional view showing the internal structure of the outer casing of the apparatus for capillary electrophoresis mass spectrometry provided in example 1;
description of reference numerals:
11-an upper cover body; 12-a housing body; 131-a mount; 132-a stop collar; 14-a glazing; 151-wrench; 152-a magnet; 153-rotation axis; 154-a rotation pin; 16-a socket; 171-a flip cushion; 172-covering a buffer piece; 181-pipeline communication holes of the electrophoresis apparatus; 182-chemical modification liquid pipeline communicating holes;
21-a support part; 211-limit mounting position; 212-first auxiliary installation site; 213-a second auxiliary mounting location; 22-a covering part; 23-top extension limiting piece; 24-a locking element;
31-an electrophoresis connection end; 32-injection end of chemical modifier; 33-electrospray output;
41-adjusting the driving end; 42-adjusting the connection end;
5-a probe cleaning mechanism;
6-a camera;
7-a display screen;
8-light source body.
Detailed Description
The technical solution of the present invention will be described clearly and completely with reference to the accompanying drawings, and obviously, the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Furthermore, the technical features mentioned in the different embodiments of the invention described below can be combined with each other as long as they do not conflict with each other.
Example 1
The present embodiment provides a biochemical analysis system, as shown in fig. 1 to 6, including: electrophoresis apparatus, mass spectrograph and capillary electrophoresis mass spectrometry equipment. Wherein, the capillary electrophoresis mass spectrometry equipment is connected between the electrophoresis apparatus and the mass spectrometer. The capillary electrophoresis mass spectrometry equipment is mechanically and electrically connected with a mass spectrometer, and is provided with a circular plug so as to introduce electrospray high-voltage electricity provided by the mass spectrometer into the capillary electrophoresis mass spectrometry equipment. Socket 16 is standard aviation plug, and the inside identification circuit that is equipped with of plug realizes being used for the mass spectrograph discernment, and the design all around of plug has the anticreep protective sheath layer.
In this embodiment, the capillary electrophoresis mass spectrometry combined equipment includes shell body, electrospray ion source, fixture, image acquisition device, display device, position adjustment mechanism, probe cleaning mechanism 5.
Wherein, the outer shell has an inner cavity isolated from the outside; the electrospray ionization source and the clamping mechanism are arranged in the inner cavity, the electrospray ionization source is communicated with the mass spectrometer and the electrophoresis apparatus, and the clamping mechanism is used for clamping the electrospray ionization source; the image acquisition device and the display device are arranged on the outer shell, and the image acquisition device acquires an image at the position of the electrospray ion source and displays and amplifies the image through the display device; the position adjusting mechanism is connected with the clamping mechanism to adjust the position of the clamping position in the inner cavity; the probe cleaning mechanism 5 is connected with an external cleaning air source and used for cleaning the electrospray ion source. Particularly, have the intercommunicating pore that communicates each other with the ion inlet of mass spectrograph on the shell body, when the actual installation uses, the inner chamber of shell body is isolated each other with the external world except that with the mass spectrograph intercommunication.
As shown in fig. 3 and 4, in the present embodiment, the electrospray ion source includes an electrophoresis connecting end 31, a chemical modification liquid injection end 32, and an electrospray output end 33. The electrophoresis connecting end 31 is communicated with an electrophoresis apparatus, and the chemical modification liquid injection end 32 is communicated with the chemical modification liquid; the electrospray output 33 is in communication with the ion inlet of the mass spectrometer.
The clamping mechanism provided in the embodiment comprises: a support part 21 and a covering part 22. Wherein, the supporting part 21 is used for supporting the electrospray ion source; the cover part 22 and the support part 21 are buckled with each other, the support part 21 and the cover part 22 form an accommodating cavity for limiting and installing the electrospray ion source, and the limiting and installing position 211 is arranged in the accommodating cavity. In this embodiment, the covering portion 22 and the supporting portion 21 are pivotally connected to each other by a rotating shaft.
As shown in fig. 4, the receiver 21 includes: a limit mounting position 211, a first auxiliary mounting position 212 and a second auxiliary mounting position 213; the electrospray output end 33 is limited and mounted in the limiting mounting position 211, and the limiting mounting position 211 is right opposite to an ion inlet of the mass spectrometer, so that the electrospray output end 33 is right opposite to a receiving port of the mass spectrometer; the first auxiliary mounting position 212 is matched with the electrophoresis connecting end 31; the second auxiliary mounting end is fitted to the chemical modification liquid injection end 32. In this embodiment, the electrospray output end 33 includes one of three-way interfaces of an electrospray ion source and one of three-way interfaces of an aggregate of electrospray nozzles, which are installed in a limiting installation position 211 (mentioned below) in a limiting manner, and the electrospray nozzle needle extends into a side of an ion inlet of a mass spectrometer. In this embodiment, the supporting portion 21 is made of an aluminum alloy material, and the covering portion 22 is made of engineering plastic, so that the insulating property is provided, and the high voltage on the electrospray nozzle needle is ensured not to leak.
For example, in the present embodiment, the first auxiliary mounting position 212, the second auxiliary mounting position 213 and the limiting mounting position 211 are limiting grooves formed on the supporting portion 21; and because electrophoresis connecting end 31, chemical modification liquid injection end 32 and electrospray output end 33 three are "T" font structure, therefore first supplementary installation position 212, second supplementary installation position 213 and spacing installation position 211 three form the transverse section and are "T" font structure.
In this embodiment, a top-extending limiting piece 23 is further included between the supporting portion 21 and the covering portion 22, and one end of the top-extending limiting piece 23 is installed at the bottom of the limiting groove and extends towards one side of the covering portion 22; the other end of the top-extending limiting part 23 abuts against the end face of one side of the electrospray ion source facing the bottom of the tank.
For example, in this embodiment, the protrusion limiting member 23 is a thimble structure made of brass, specifically, the capillary electrophoresis mass spectrometry apparatus receives a high voltage introduced from one side of the mass spectrometer, and is connected to the supporting portion 21 through a protection resistor and a high voltage resistant wire arranged in the capillary electrophoresis mass spectrometry apparatus, and since the thimble structure protrudes from the supporting portion 21, the thimble structure serves as a conductive contact of the electrospray high voltage, thereby protecting the stability of the electrical connection. In addition, the electrospray ion source is stably clamped between the contact structure of the top extension limiting part 23 and the cover part 22, and the installation stability of the electrospray ion source is further ensured.
According to the size of the protection resistor and the diameter and the bendable degree of the high-voltage-resistant wire, a resistor wire fixing piece, a resistor pressing plate, a wire fixing pressing plate, a wire groove and a protective cover are designed in the capillary electrophoresis mass spectrometry equipment, so that insulation is ensured, the protection resistor and the wire are hidden inside, and the appearance of the capillary electrophoresis mass spectrometry equipment is attractive.
As shown in fig. 3, the clamping mechanism further includes a locking member 24, and the locking member 24 is installed between the covering portion 22 and the supporting portion 21 for locking the closing between the covering portion 22 and the supporting portion 21. Specifically, the locking member 24 in the present embodiment is a magnetic attraction member, such as a magnet, which is mounted on the cover portion 22 and the receiver portion 21, respectively.
The position adjusting mechanism provided by the embodiment comprises an adjusting driving end 41 arranged on the outer side of the outer shell and an adjusting connecting end 42 connected with the clamping mechanism, wherein the adjusting driving end 41 drives the adjusting connecting end 42 to move in the inner cavity. In particular, the present embodiment provides a displacement adjustment mechanism that enables precise positional adjustment of the electrospray output 33 with respect to the ion inlet of the mass spectrometer.
For example, in this embodiment, the adjusting connection end 42 is made of engineering plastics, so as to ensure the insulation property of the adjusting connection end. The adjusting driving end 41 is made of aluminum alloy material. The initial distance of the electrospray output 33 relative to the ion inlet of the mass spectrometer is 8 mm, and the electrospray output 33 is coaxial with the centre line of the ion inlet of the mass spectrometer. The adjustment driving end 41 is adjusted to adjust the adjusting connecting end 42 to achieve an adjusting range larger than 6 mm in three directions perpendicular to each other. In this embodiment, the two adjusting driving ends 41 are arranged at one end far away from the mass spectrometer, and the other adjusting driving end 41 is arranged on the left side wall surface of the outer shell, so that the position of the electrospray output end 33 of the electrospray ion source relative to the ion inlet of the mass spectrometer is adjusted in multiple dimensions, and the stability and the sensitivity of the electrospray ionization process are ensured.
The capillary electrophoresis mass spectrometry apparatus provided in this embodiment, as shown in fig. 2, further includes a probe cleaning mechanism 5, which includes a connecting pipe extending into the inner cavity, one end of the connecting pipe is connected to a cleaning air source provided by the mass spectrometry, the other end of the connecting pipe has a spraying port near the electrospray output end 33, and the spraying port releases cleaning air toward the electrospray output end 33.
For example, the cleaning gas in this embodiment is nitrogen. In the probe cleaning mechanism 5 with the structure, the electrospray output end 33 is purged on line through the probe cleaning mechanism 5, so that the outer shell does not need to be opened, any possible ion source pollution can not be introduced, and the cleanliness of the capillary electrophoresis mass spectrometry combination equipment is ensured.
The image acquisition device provided by the embodiment comprises a processor and a camera 6 for acquiring an image of an electrospray output end 33, wherein the camera 6 is electrically connected with the processor and used for acquiring the image and outputting the image to the processor; the display device is electrically connected with the image acquisition device and is used for displaying the image acquired by the camera 6. The camera in this embodiment is a microscopic camera.
Specifically, as shown in fig. 2 and 5, the camera 6 is mounted in a mounting position on an inner side wall surface of the outer housing, specifically, the outer housing is provided with a mounting piece 131 and a limiting sleeve 132, wherein the mounting piece 131 of the camera 6 is made of metal aluminum, and the limiting sleeve 132 for mounting the camera 6 is made of rubber. The camera 6 is installed firmly, and the center of the camera observation is an electrospray Taylor cone at the electrospray output end 33. The observation axis of the camera 6 deviates 70 degrees to the right relative to the axis of the electrospray nozzle needle.
In this embodiment, the display device is a 4.5 inch display screen 7 disposed on the side of the outer housing away from the mass spectrometer, specifically, the display screen 7 has a resolution 480 x 854. The length of the display 7 on the left and below, to the nearest millimeter, can assist in estimating the distance of the electrospray needle relative to the mass spectrometer entrance. Of course, in other embodiments, the display 7 may present the specific distance value calculated earlier by the computer according to the photographic magnification ratio.
Of course, in other embodiments, the display device may also be a computer independently disposed outside the outer casing, and the display of the image obtained by the image capturing device may also be realized.
The capillary electrophoresis mass spectrometry apparatus further includes a light source 8 disposed in the inner cavity to improve the brightness of the electrospray output end 33. The arrangement of the light source body 8 is mainly because the camera 6 in the embodiment is a micro camera 6, and the micro camera 6 causes the image to be dark and unclear due to the amplification effect. Therefore, the light source body 8 is arranged to achieve the purpose of light supplement. In this embodiment, the light source body 8 is the high brightness LED light that sets up in the inner chamber, and power is 5 watts, and the back is opened to the LED light, can effectively improve microscopic image's detail definition, and in this embodiment, LED light and its internal circuit integration are in the inside of casing to guarantee that the structure is pleasing to the eye durable.
As shown in fig. 1 and 2, the outer case includes an upper cover 11 and a case body 12, and the upper cover 11 is arranged on the case body 12 in a foldable manner. The pivoting rotary shaft is arranged on the left side of the outer shell, a covering buffer member 172 is arranged on any side without the rotary shaft, specifically, the covering buffer member is made of 172 rubber materials, opening and closing vibration and noise can be effectively reduced, the durability of the system is improved, a folding buffer member 171 is further arranged on the shell body on one side where the pivoting rotary shaft is arranged, the folding buffer member is a limiting lug which is made of rubber materials and is arranged from the shell body 12 to one side of the upper cover body 11 in a protruding mode, the opening and closing angle of the upper cover body is limited by the limiting lug, and impact on the camera 6 in the folding process is further avoided; a transparent glass window 14 is further arranged on the right side of the upper cover body 11 and is used for assisting an operator to directly observe the position of the spray needle and the working state of the electric spray; four gaskets are arranged at the bottom of the shell body 12 and can be made of rubber materials, so that the capillary electrophoresis mass spectrometry combined equipment can be stably and horizontally placed on a plane.
In this embodiment, as shown in fig. 2, 3 and 6, two mounting buckle wrenches 151, a wrench 151, two pairs of magnets 152 and a magnet 152 are respectively disposed on two sides of the outer casing. Specifically, the outer shell and the wrench 151 are pivoted through a rotating shaft 153, one side of the rotating shaft 153 close to the mass spectrometer is provided with a rotating pin 154, and the rotating pin 154 is used for locking and connecting the mass spectrometer and the outer shell; the rotation shaft 153 and rotation pin 154 may be connected to a mass spectrometer mounting site. For example, when the wrench 151 is in the open state, the rotation shaft 153 is inserted into the mass spectrometer mounting site; when the wrench 151 is turned until the wrench 151 and the magnet 152 are mutually attracted, the turning pin 154 at the end of the turning shaft 153 is turned by the turning shaft 153, and the turning pin 154 is limited and locked in the installation position of the mass spectrometer. At this time, the wrench 151 and the magnet 152 are ensured to be attracted to each other, so that the connection stability between the capillary electrophoresis mass spectrometry equipment and the mass spectrometer can be ensured.
In this embodiment, as shown in fig. 1, an electrophoresis apparatus conduit communication hole 181 and a chemical modification liquid conduit communication hole 182 are further provided between the upper cover 11 and the housing body 12. The electrophoresis apparatus conduit communication hole 181 is used to accommodate the conduit through which the electrophoresis apparatus and the electrophoresis connection end communicate with each other, and the chemical modification liquid conduit communication hole 182 is used to accommodate the conduit through which the chemical modification liquid injection end 32 and the chemical modification liquid injection pump communicate with each other.
In this embodiment, the capillary electrophoresis mass spectrometry apparatus is connected to an external 220V-to-12V power module through a power plug, so as to ensure power supply.
The present embodiment provides a biochemical analysis system, which when in use: s1: open the installation spanner 151 of shell body both sides, install capillary electrophoresis mass spectrometry combination equipment on the mass spectrometer, the firm absorption of magnet 152 and spanner 151's magnet 152 on the lock both sides installation spanner 151 until casing body 12.
S2: installing a micro camera 6 in an installation vacancy at the left side of an ion source;
s3: assembling the electrospray ion source, opening the upper cover body 11, then opening the cover arrangement part 22, installing the electrospray ion source in the limiting groove, and ensuring that the electrospray output end 33 is installed in the limiting installation end; the electrophoresis connection terminal 31 is installed in the first auxiliary installation site 212; the chemical modification solution injection end 32 is installed in the second auxiliary installation site 213. Thereafter, the lid-mounting portion 22 and the upper lid body 11 are sequentially closed.
S4: and pressing a power switch button of the capillary electrophoresis mass spectrometry equipment. The position of the electrospray ion source is adjusted by controlling the adjusting driving end 41 to drive the adjusting connecting end 42 according to the relative position of the electrospray spray needle monitored by the front upper side display screen 7 and the position of the electrospray output end 33 observed in the right transparent glass window 14.
S5: the chemical modifier injection port 32 is connected to an injection pump attached to the upper portion of the mass spectrometer.
So far, the capillary electrophoresis mass spectrometry equipment is installed, and the CE and mass spectrometry parameters can be set according to a specific sample analysis task and then the equipment is used.
According to the capillary electrophoresis mass spectrometry combined equipment provided by the embodiment, through the arrangement of the closed inner cavity, the disturbance of the outside on the output of the electrospray ion source is reduced, and the accuracy of a mass spectrometry detection signal is improved; meanwhile, in the clamping mechanism, the arrangement of the limiting installation position 211 can further ensure that the electrospray ion sources are correspondingly fixed with the mass spectrometer, so that the installation stability of the electrospray ion source is greatly improved and the analysis result is prevented from generating errors in the use process of the ion source; meanwhile, when the capillary electrophoresis mass spectrometry combination equipment is used, the electrospray ion source can be directly installed through the clamping mechanism, and the continuous disassembly of the electrospray ion source from the installation position is avoided, so that the reliability of installation between the limiting installation position and the electrospray output end is ensured, and the occurrence of analysis result errors is avoided. It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications can be made without departing from the scope of the invention.
Claims (10)
1. A capillary electrophoresis mass spectrum coupling device is connected between an electrophoresis apparatus and a mass spectrometer; it is characterized by comprising:
the outer shell is provided with an inner cavity isolated from the outside;
the electrospray ionization source is provided with an electrophoresis connecting end (31), a chemical modification liquid injection end (32) and an electrospray output end (33), the electrophoresis connecting end (31) is communicated with an electrophoresis apparatus, and the chemical modification liquid injection end (32) is communicated with a chemical modification liquid;
the electrospray ion source is suitable for being clamped in the clamping mechanism, the clamping mechanism is provided with a limiting installation position (211), the electrospray output end (33) is installed in the limiting installation position (211) in a limiting mode, and the limiting installation position (211) is right opposite to an ion inlet of a mass spectrometer.
2. The capillary electrophoresis mass spectrometry apparatus of claim 1 wherein said clamping mechanism comprises: a support part (21) for supporting the electrospray ion source;
and the cover arranging part (22) is buckled with the bearing part (21), the bearing part (21) and the cover arranging part (22) form an accommodating cavity for installing the electrospray ion source at a limited position, and the limiting installation position (211) is arranged in the accommodating cavity.
3. The combined capillary electrophoresis and mass spectrometry apparatus of claim 2 wherein said receiving chamber further comprises a first auxiliary mounting location (212) coupled to said electrophoresis connection end (31) and a second auxiliary mounting location (213) coupled to said chemical modification solution injection end (32);
the first auxiliary mounting position (212), the second auxiliary mounting position (213) and the limiting mounting position (211) are limiting grooves formed in the bearing part (21).
4. The capillary electrophoresis mass spectrometry apparatus according to claim 3, wherein the clamping mechanism further comprises at least one top-extension limiting member (23), one end of the top-extension limiting member (23) is installed at the bottom of the limiting groove and extends towards one side of the covering portion (22); the other end of the jacking limiting part (23) abuts against the end face, facing the bottom of the tank, of one side of the electrospray ion source.
5. The combined capillary electrophoresis and mass spectrometry apparatus of claim 2, wherein said clamping mechanism further comprises at least one locking member (24), said locking member (24) being mounted between said cover portion (22) and said support portion (21) for locking closure between said cover portion (22) and said support portion (21).
6. The combined capillary electrophoresis and mass spectrometry apparatus of any one of claims 1 to 5, further comprising a position adjustment mechanism connected to the clamping mechanism,
the clamping mechanism comprises an adjusting driving end (41) arranged on the outer side of the outer shell and an adjusting connecting end (42) connected with the clamping mechanism, wherein the adjusting driving end (41) drives the adjusting connecting end (42) to move in the inner cavity.
7. The capillary electrophoresis mass spectrometry apparatus of any one of claims 1 to 5,
the electrospray ionization device further comprises an image acquisition device, wherein the image acquisition device is provided with a processor and a camera (6) for acquiring an image of the electrospray output end (33), and the camera (6) is electrically connected with the processor;
the display device is arranged on the outer shell and electrically connected with the image acquisition device, and the display device is used for displaying the image acquired by the camera (6).
8. The combined capillary electrophoresis and mass spectrometry apparatus of claim 7, further comprising a light source body (8) disposed within said internal cavity to increase the brightness of the output image at the electrospray output end (33) side.
9. The combined capillary electrophoresis and mass spectrometry apparatus according to any one of claims 1 to 5, wherein the outer casing comprises an upper cover body (11) and a casing body (12), and the upper cover body (11) is foldably arranged on the casing body (12).
10. A biochemical analysis system, comprising:
an electrophoresis apparatus and a mass spectrometer;
the capillary electrophoresis mass spectrometry apparatus of any one of claims 1 to 9, further comprising a capillary electrophoresis mass spectrometry apparatus connected between the electrophoresis apparatus and the mass spectrometer.
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| CN111243937A (en) * | 2020-03-11 | 2020-06-05 | 知健生物分子研究院(南京)有限公司 | Capillary electrophoresis mass spectrometry combined equipment and biochemical analysis system |
| CN111243937B (en) * | 2020-03-11 | 2024-05-24 | 知健生物分子研究院(南京)有限公司 | Capillary electrophoresis mass spectrometry equipment and biochemical analysis system |
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