CN211420189U - Cell culture device for bio-pharmaceuticals - Google Patents
Cell culture device for bio-pharmaceuticals Download PDFInfo
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- CN211420189U CN211420189U CN201921590662.3U CN201921590662U CN211420189U CN 211420189 U CN211420189 U CN 211420189U CN 201921590662 U CN201921590662 U CN 201921590662U CN 211420189 U CN211420189 U CN 211420189U
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Abstract
The utility model discloses a cell culture device for bio-pharmaceuticals, which comprises a box body, a ventilation unit, a humidification unit and a carbon dioxide unit; the inside of box is cavity, the inside bottom of box is equipped with the culture dish, the inside trailing flank of box is equipped with three ultraviolet tube, and the both ends of three ultraviolet tube are fixed at the inside trailing flank of box through two mounting panels, the front side of box is equipped with the chamber door, and the right-hand member of chamber door rotates through the left end of pivot with the box leading flank to be connected, the upper end of the inside left surface of box is equipped with temperature sensor and humidity transducer, the upper end of the inside right flank of box is equipped with the carbon dioxide detector, and this cell culture device for bio-pharmaceuticals can the concentration of internal temperature of automated inspection case, humidity and carbon dioxide to automatic adjustment has alleviateed staff's burden, and can be at the growth condition of incasement direct observation cell.
Description
Technical Field
The utility model relates to a cell culture technical field specifically is a cell culture device for bio-pharmaceuticals.
Background
The cell culture technology can be used for preparing simple single cells or rarely differentiated multiple cells from one cell through mass culture, which is an indispensable link of the cloning technology, the cell culture is the cloning of the cell, the cell culture technology is an important and common technology in a cell biology research method, a large number of cells can be obtained through the cell culture, and the signal transduction of the cells, the anabolism of the cells, the growth and the proliferation of the cells and the like can be researched by the cell culture technology, at present, a vessel for the cell culture needs to be sterilized and then put into an incubator for culture, but the current incubator can only adjust the temperature, humidity and carbon dioxide concentration before the vessel is put into the incubator, and the internal temperature, humidity and carbon dioxide concentration are detected at any moment in the culture process and need to be adjusted in real time, the manual adjustment is very troublesome, for example, if the adjustment is wrong or the cultured cells are not detected for a long time, the cultured cells can die in a large amount, and the culture dish is frequently taken out to observe the growth condition of the cells, so that the cells in the culture dish can be easily infected with germs.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to overcome current defect, provide a cell culture device for bio-pharmaceuticals, but the concentration of temperature, humidity and carbon dioxide in the automated inspection box to automatic adjustment has alleviateed staff's burden, and can be at the growth condition of incasement direct observation cell, can effectively solve the problem in the background art.
In order to achieve the above object, the utility model provides a following technical scheme: a cell culture device for bio-pharmaceuticals comprises a box body, a ventilation unit, a humidification unit and a carbon dioxide unit;
a box body: the box body is hollow, a culture dish is arranged at the bottom inside the box body, three ultraviolet lamp tubes are arranged on the rear side surface inside the box body, two ends of each ultraviolet lamp tube are fixed on the rear side surface inside the box body through two mounting plates, a box door is arranged on the front side of the box body, the right end of the box door is rotatably connected with the left end of the front side surface of the box body through a rotating shaft, a temperature sensor and a humidity sensor are arranged at the upper end of the left side surface inside the box body, a carbon dioxide detector is arranged at the upper end of the right side surface inside the box body, and heating wires are arranged in a;
a ventilation unit: the air exchange unit comprises a one-way air outlet valve, a high-efficiency filter, an induced draft fan and an air pipe, wherein the one-way air outlet valve is arranged at the upper end of the right side face of the box body;
a humidifying unit: the humidifying unit comprises a steam pipe, an exhaust fan, an electric heating pipe and a water tank, wherein the water tank is arranged at the lower end of the left side face of the box body, the exhaust fan is arranged in the middle of the upper surface of the water tank, an air inlet of the exhaust fan is connected with a steam outlet of the water tank, the upper end of the steam pipe is connected with the middle of the left side face of the box body, the steam pipe is communicated with the inside of the box body, the lower end of the steam pipe is connected with an air outlet of the exhaust fan, the electric heating pipe is arranged in the middle of the rear side face of the water tank;
a carbon dioxide unit: the carbon dioxide unit comprises a carbon dioxide storage tank, a connecting pipe and an electromagnetic valve, the carbon dioxide storage tank is arranged on the left side of the upper surface of the box body, the upper end of the connecting pipe is connected with an air outlet of the carbon dioxide storage tank, the lower end of the connecting pipe is connected with the middle part of the upper surface of the box body, the lower end of the connecting pipe is communicated with the interior of the box body, and the electromagnetic valve is arranged on the connecting pipe;
wherein: still include atomizer and PLC, atomizer establishes at the inside left surface middle part of box, and atomizer's air inlet and steam pipe intercommunication, PLC establishes the middle part at the box upper surface, and PLC's input is connected with external power source's output electricity, PLC's output is connected with draught fan, air exhauster, electrothermal tube, solenoid valve, ultraviolet tube and heating wire's input electricity respectively, temperature sensor, humidity transducer and carbon dioxide detector's output is connected with PLC's input electricity, but through temperature sensor, humidity transducer and carbon dioxide detector real-time detection incasement temperature, humidity and carbon dioxide's concentration to adjust through PLC automatic control heating wire, ventilation unit, humidification unit and carbon dioxide unit to the temperature in the box, humidity and carbon dioxide's concentration.
Further, still include the sealing strip, the sealing strip is the annular, and the sealing strip is established at the leading flank of chamber door, can increase the leakproofness of box through the sealing strip.
Further, still include electron microscope, electron microscope establishes the top in the box, and electron microscope is located the culture dish directly over, electron microscope's output is connected with PLC's input electricity, can be in the box direct observation cell's the growth condition through electron microscope.
Further, still including cotton and the linker of absorbing water, the cotton top of establishing in the box that absorbs water, the linker is established in the side of water tank, and the inside of linker intercommunication water tank, can prevent through the cotton that absorbs water that the box top from forming the condensation and dripping in the culture dish, can observe the water level in the water tank through the linker, prevents that the water in the water tank from burning dry and damaging the electrothermal tube.
The box door is characterized by further comprising a touch display, the touch display is arranged in the middle of the rear side face of the box door and is electrically connected with the PLC in a bidirectional mode, and images observed by the electron microscope can be output through the touch display.
Compared with the prior art, the beneficial effects of the utility model are that: this cell culture device for bio-pharmaceuticals has following advantage:
1. but through temperature sensor, humidity transducer and carbon dioxide detector real-time detection incasement temperature, humidity and the concentration of carbon dioxide to adjust through PLC automatic control heating wire, the unit of taking a breath, humidification unit and carbon dioxide unit temperature, humidity and the concentration of carbon dioxide in to the box.
2. The growth condition of cells can be directly observed in the box body through an electron microscope, and images can be output through the touch display.
3. Through the leakproofness of sealing strip multiplicable box, can prevent through the cotton that absorbs water that the box top from forming the condensation and drippage in the culture dish, can observe the water level in the water tank through the linker, prevent that the water in the water tank from heating up futilely and damaging the electrothermal tube.
Drawings
FIG. 1 is a schematic front view of the present invention;
FIG. 2 is a right side schematic view of the present invention;
FIG. 3 is a left side schematic view of the present invention;
fig. 4 is a schematic view of the cross-sectional structure of the present invention.
In the figure: the device comprises a box body 1, a ventilation unit 2, a one-way air outlet valve 21, a high-efficiency filter 22, an induced draft fan 23, an air pipe 24, a humidification unit 3, a steam pipe 31, an induced draft fan 32, an electric heating pipe 33, a water tank 34, a carbon dioxide unit 4, a carbon dioxide storage tank 41, a connecting pipe 42, an electromagnetic valve 43, a culture dish 5, a mounting plate 6, an ultraviolet lamp tube 7, an atomizing nozzle 8, a box door 9, a rubber sealing strip 10, a temperature sensor 11, a humidity sensor 12, an electronic microscope 13, a PLC14, a water absorption cotton 15, a carbon dioxide detector 16, a touch display 17, an electric heating wire 18.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution: a cell culture device for bio-pharmaceuticals comprises a box body 1, a ventilation unit 2, a humidification unit 3 and a carbon dioxide unit 4;
a box body 1: the interior of the box body 1 is hollow, a culture dish 5 is arranged at the bottom of the interior of the box body 1, three ultraviolet lamp tubes 7 are arranged on the rear side face of the interior of the box body 1, two ends of each ultraviolet lamp tube 7 are fixed on the rear side face of the interior of the box body 1 through two mounting plates 6, a box door 9 is arranged on the front side of the box body 1, the right end of the box door 9 is rotatably connected with the left end of the front side face of the box body 1 through a rotating shaft, a temperature sensor 11 and a humidity sensor 12 are arranged at the upper end of the left side face of the interior of the box body 1, a carbon dioxide detector 16 is arranged at the;
the ventilation unit 2: the air exchange unit 2 comprises a one-way air outlet valve 21, a high-efficiency filter 22, an induced draft fan 23 and an air pipe 24, the one-way air outlet valve 21 is arranged at the upper end of the right side surface of the box body 1, the high-efficiency filter 22 is arranged in the middle of the right side surface of the box body 1, the front end of the air pipe 24 is connected with an air outlet of the high-efficiency filter 22, the rear end of the air pipe 24 is connected with the right side surface of the box body 1, the rear end of the air pipe 24 is communicated with the inside of the box body 1, the induced draft fan 23 is arranged on the air pipe;
the humidifying unit 3: the humidifying unit 3 comprises a steam pipe 31, an exhaust fan 32, an electric heating pipe 33 and a water tank 34, the water tank 34 is arranged at the lower end of the left side surface of the box body 1, the exhaust fan 32 is arranged in the middle of the upper surface of the water tank 34, an air inlet of the exhaust fan 32 is connected with a steam outlet of the water tank 34, the upper end of the steam pipe 31 is connected with the middle of the left side surface of the box body 1, the steam pipe 31 is communicated with the inside of the box body 1, the lower end of the steam pipe 31 is connected with an air outlet of the exhaust fan 32, the electric heating pipe 33 is arranged in the middle of the rear side surface of the water tank;
carbon dioxide unit 4: the carbon dioxide unit 4 comprises a carbon dioxide storage tank 41, a connecting pipe 42 and an electromagnetic valve 43, wherein the carbon dioxide storage tank 41 is arranged on the left side of the upper surface of the box body 1, the upper end of the connecting pipe 42 is connected with an air outlet of the carbon dioxide storage tank 41, the lower end of the connecting pipe 42 is connected with the middle part of the upper surface of the box body 1, the lower end of the connecting pipe 42 is communicated with the inside of the box body 1, and the electromagnetic valve 43 is arranged on the connecting;
wherein: also comprises an atomizing nozzle 8 and a PLC14, the atomizing nozzle 8 is arranged in the middle of the left side surface in the box body 1, and the air inlet of the atomizing nozzle 8 is communicated with the steam pipe 31, the PLC14 is arranged in the middle of the upper surface of the box body 1, the input end of the PLC14 is electrically connected with the output end of an external power supply, the output end of the PLC14 is respectively and electrically connected with the input ends of the draught fan 23, the exhaust fan 32, the electric heating tube 33, the electromagnetic valve 43, the ultraviolet lamp tube 7 and the electric heating wire 18, the output ends of the temperature sensor 11, the humidity sensor 12 and the carbon dioxide detector 16 are electrically connected with the input end of the PLC14, the temperature, humidity and carbon dioxide concentration in the cabinet 1 can be detected in real time by the temperature sensor 11, the humidity sensor 12 and the carbon dioxide detector 16, and the temperature, humidity and carbon dioxide concentration in the box body 1 are adjusted by automatically controlling the heating wire 18, the ventilation unit 2, the humidification unit 3 and the carbon dioxide unit 4 through the PLC 14.
Wherein: the box body 1 further comprises a sealing strip 10, the sealing strip 10 is annular, the sealing strip 10 is arranged on the front side face of the box door 9, and the sealing performance of the box body 1 can be improved through the sealing strip 10.
Wherein: still include electron microscope 13, electron microscope 13 establishes the top in box 1, and electron microscope 13 is located the culture dish 5 directly over, and electron microscope 13's output is connected with PLC 14's input electricity, can directly observe the growth condition of cell in box 1 through electron microscope 13.
Wherein: the water-absorbing cotton box further comprises water-absorbing cotton 15 and a communicating vessel 19, the water-absorbing cotton 15 is arranged at the top in the box body 1, the communicating vessel 19 is arranged on the side face of the water tank 34, the communicating vessel 19 is communicated with the inside of the water tank 34, condensation formed at the top of the box body 1 can be prevented from dripping into the culture dish 5 through the water-absorbing cotton 15, the water level in the water tank 34 can be observed through the communicating vessel 19, and the phenomenon that the electrothermal tube 33 is damaged due to the fact that water in the water tank 34 is dried out is prevented.
Wherein: the box door structure further comprises a touch display 17, wherein the touch display 17 is arranged in the middle of the rear side face of the box door 9, the touch display 17 is electrically connected with the PLC14 in a bidirectional mode, and images observed by the electron microscope 13 can be output through the touch display 17.
When in use: an external power supply is connected, the operation of the ultraviolet lamp tube 7 is automatically controlled by the PLC14, the ultraviolet generated by the ultraviolet lamp tube 7 disinfects and sterilizes the interior of the box body 1, the temperature, the humidity and the concentration of carbon dioxide required by cell culture are adjusted by the touch display 17, the cell tissue to be cultured is placed in the culture dish 5, when the temperature sensor 11 detects that the temperature in the box body 1 is less than a set value in the cell culture process, the PLC14 automatically controls the heating wire 18 to heat until the temperature in the box reaches the set value, the PLC14 automatically controls the heating wire 18 to stop working, when the temperature sensor 12 detects that the temperature in the box body 1 is less than the set value, the PLC14 automatically controls the electric heating tube 33 to heat, the water in the water tank 34 generates steam, meanwhile, the PLC14 automatically controls the exhaust fan 32 to work, and the steam is conveyed to the interior of the box body, and is sprayed out through the atomizing nozzle 8, when the carbon dioxide detector 16 detects that the concentration of carbon dioxide in the box body 1 is less than a set value, the PLC14 automatic control electromagnetic valve 43 is opened, carbon dioxide in the carbon dioxide storage tank 41 is conveyed to the inside of the box body 1 through the connecting pipe 42, when the temperature sensor 11, the humidity sensor 12 and the carbon dioxide detector 16 detect that the temperature, the humidity and the concentration of carbon dioxide in the inside are greater than the set value, the PLC14 automatic control induced draft fan 23 works, after impurities and bacteria are filtered out through the high efficiency filter 22, the air is conveyed to the inside of the box body 1 through the air pipe 24, and the one-way air outlet valve 21 of the gas barrel in the box body 1 is.
It is to be noted that the induced draft fan 23 used in this embodiment is proposed to be a centrifugal fan of the electrical limited company of hong Kong, Leqing, the specific model is Y5-47, the induced draft fan 32 is proposed to be a pipeline fan of the electrical limited company of hong Guang, Shenzhen, the specific model is HG-HF-150P, the model of the temperature sensor 11 is pt100, the humidity sensor 12 can be an infrared sensor of OMEGA, the model of the carbon dioxide detector 16 is RS-CO2, the PLC14 is proposed to be a PLC of the finite company of Emustion technologies, Hangzhou, the specific model is 223-1PH22-0XA8, the electronic microscope 13 is proposed to be an electronic microscope with the finite technology of Shenzhen Andong, the specific model is DP-5, and the PLC14 controls the induced draft fan 23, the induced draft fan 32 and, the solenoid valve 43, the ultraviolet lamp tube 7 and the heating wire 18 are operated in a conventional manner in the art.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. A cell culture apparatus for bio-pharmaceuticals, comprising: comprises a box body (1), a ventilation unit (2), a humidification unit (3) and a carbon dioxide unit (4);
case (1): the box body (1) is hollow, a culture dish (5) is arranged at the bottom inside the box body (1), three ultraviolet lamp tubes (7) are arranged on the rear side face inside the box body (1), two ends of each ultraviolet lamp tube (7) are fixed to the rear side face inside the box body (1) through two mounting plates (6), a box door (9) is arranged on the front side of the box body (1), the right end of the box door (9) is rotatably connected with the left end of the front side face of the box body (1) through a rotating shaft, a temperature sensor (11) and a humidity sensor (12) are arranged at the upper end of the left side face inside the box body (1), a carbon dioxide detector (16) is arranged at the upper end of the right side face inside the box body (1), and an electric heating wire (18) is arranged in a cavity;
ventilation unit (2): the air exchange unit (2) comprises a one-way air outlet valve (21), a high-efficiency filter (22), an induced draft fan (23) and an air pipe (24), the one-way air outlet valve (21) is arranged at the upper end of the right side face of the box body (1), the high-efficiency filter (22) is arranged in the middle of the right side face of the box body (1), the front end of the air pipe (24) is connected with an air outlet of the high-efficiency filter (22), the rear end of the air pipe (24) is connected with the right side face of the box body (1), the rear end of the air pipe (24) is communicated with the inside of the box body (1), the induced draft fan (23) is arranged on the air pipe (24), and the side face of the induced draft fan (;
humidification unit (3): the humidifying unit (3) comprises a steam pipe (31), an exhaust fan (32), an electric heating pipe (33) and a water tank (34), the water tank (34) is arranged at the lower end of the left side face of the box body (1), the exhaust fan (32) is arranged in the middle of the upper surface of the water tank (34), an air inlet of the exhaust fan (32) is connected with a steam outlet of the water tank (34), the upper end of the steam pipe (31) is connected with the middle of the left side face of the box body (1), the steam pipe (31) is communicated with the inside of the box body (1), the lower end of the steam pipe (31) is connected with an air outlet of the exhaust fan (32), the electric heating pipe (33) is arranged in the middle of the rear side face of the water tank (34), and the front end of the electric heating pipe (33) penetrates through the rear side face;
carbon dioxide unit (4): the carbon dioxide unit (4) comprises a carbon dioxide storage tank (41), a connecting pipe (42) and an electromagnetic valve (43), the carbon dioxide storage tank (41) is arranged on the left side of the upper surface of the box body (1), the upper end of the connecting pipe (42) is connected with an air outlet of the carbon dioxide storage tank (41), the lower end of the connecting pipe (42) is connected with the middle of the upper surface of the box body (1), the lower end of the connecting pipe (42) is communicated with the inside of the box body (1), and the electromagnetic valve (43) is arranged on the connecting pipe (42);
wherein: still include atomizer (8) and PLC (14), the left surface middle part inside box (1) is established in atomizer (8), and the air inlet and steam pipe (31) intercommunication of atomizer (8), the middle part at box (1) upper surface is established in PLC (14), and the input of PLC (14) is connected with external power source's output electricity, the output of PLC (14) is connected with the input electricity of draught fan (23), air exhauster (32), electrothermal tube (33), solenoid valve (43), ultraviolet tube (7) and heating wire (18) respectively, the output of temperature sensor (11), humidity transducer (12) and carbon dioxide detector (16) is connected with the input electricity of PLC (14).
2. A cell culture apparatus as claimed in claim 1, wherein: the refrigerator door is characterized by further comprising a sealing strip (10), wherein the sealing strip (10) is annular, and the sealing strip (10) is arranged on the front side face of the refrigerator door (9).
3. A cell culture apparatus as claimed in claim 1, wherein: still include electron microscope (13), electron microscope (13) are established at the top in box (1), and electron microscope (13) are located culture dish (5) directly over, the output of electron microscope (13) is connected with PLC (14)'s input electricity.
4. A cell culture apparatus as claimed in claim 1, wherein: still including cotton (15) and the linker (19) absorb water, the top in box (1) is established in cotton (15) absorb water, the side at water tank (34) is established in linker (19), and inside of linker (19) intercommunication water tank (34).
5. A cell culture apparatus as claimed in claim 1, wherein: the refrigerator door further comprises a touch display (17), wherein the touch display (17) is arranged in the middle of the rear side face of the refrigerator door (9), and the touch display (17) is electrically connected with the PLC (14) in a bidirectional mode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921590662.3U CN211420189U (en) | 2019-09-24 | 2019-09-24 | Cell culture device for bio-pharmaceuticals |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921590662.3U CN211420189U (en) | 2019-09-24 | 2019-09-24 | Cell culture device for bio-pharmaceuticals |
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| Publication Number | Publication Date |
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| CN211420189U true CN211420189U (en) | 2020-09-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201921590662.3U Active CN211420189U (en) | 2019-09-24 | 2019-09-24 | Cell culture device for bio-pharmaceuticals |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112833643A (en) * | 2021-01-21 | 2021-05-25 | 梁庆开 | Electric heat blast air drying cabinet for cell culture |
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2019
- 2019-09-24 CN CN201921590662.3U patent/CN211420189U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112833643A (en) * | 2021-01-21 | 2021-05-25 | 梁庆开 | Electric heat blast air drying cabinet for cell culture |
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Effective date of registration: 20201229 Address after: 201100 Room 201, building 2, 2350 Duzhuang Road, Minhang District, Shanghai Patentee after: Shanghai ruibaohe Biotechnology Co.,Ltd. Address before: 230031 Room 601, building 13, cuiweiyuan, Furong community, Shushan District, Hefei City, Anhui Province Patentee before: Su Dangsheng |