CN211339510U

CN211339510U - Cell disruption device

Info

Publication number: CN211339510U
Application number: CN201921989940.2U
Authority: CN
Inventors: 林强; 黄晓飞; 洪立鑫
Original assignee: Hubei Youliankang Biotechnology Co ltd
Current assignee: Hubei Youliankang Biotechnology Co ltd
Priority date: 2019-11-18
Filing date: 2019-11-18
Publication date: 2020-08-25
Anticipated expiration: 2029-11-18

Abstract

The utility model discloses a cell crushing device, which comprises a shell, a crushing ball, a crushing tank, a rotating rod, an impact plate and a motor, wherein the motor drives the rotating rod and the stirring paddle to rotate, so that the stirring paddle can be in contact with the crushing ball to push, cell suspension and the crushing ball are mixed and stirred, the crushing ball and a convex scribing sheet on the surface of the crushing ball have the effects of high-speed impact, cutting, extrusion, cutting and the like on hard cells, an ideal crushing effect can be achieved in a very short time, materials are fluidized in the crushing process, each particle has the same stress state, the effect of precise mixing is achieved during crushing, the cell suspension inside the crushing tank can be circularly led out and input through a circulating pump, the flowing speed and the flowing strength of the cell suspension inside the crushing tank are increased, in the high-speed flowing process, the cell suspension is under the effect of the impact plate, the impact effect is generated, so that the cells in the cell suspension have the primary crushing effect.

Description

Cell disruption device

Technical Field

The utility model relates to the technical field of cells, specifically a cell disruption device.

Background

The cell disruption technology is a technology for destroying cell membranes and cell walls by using external force to release target product components from cell contents, when a certain protein is disrupted, separated and purified, the protein is firstly released from tissues or cells and is kept in an original natural state without losing activity, so that the tissues and the cells are disrupted by adopting a proper method, the cell disruption difficulty is different for different organisms or tissues of different parts of the same organism, the use method is different, for example, the cell membranes of animal organs are fragile and easy to disrupt, and plants and microorganisms have firmer cell walls consisting of cellulose and hemicellulose.

The existing cell disruption method is single, low in disruption efficiency and incapable of realizing rapid and effective cell disruption effect, and the protein separated after cell disruption is easy to lose activity due to long disruption time.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a cell disruption device to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a cell crushing device, includes the casing, the inboard of casing is rotated through the bearing and is installed the dwang, the surface of dwang evenly is provided with the stirring rake, the inboard of casing is provided with broken ball, the inside bottom fixed mounting of casing has the separation net, the below fixed mounting of separation net has toper water conservancy diversion chamber, the discharge port has been seted up to the bottom in toper water conservancy diversion chamber, the motor is installed through the fixed plate to a side surface of casing, the output of motor runs through the housing face and is connected with the one end of dwang, the top of casing is provided with the water conservancy diversion mouth, water conservancy diversion mouth and discharge port department all are provided with the control valve, the top fixed mounting of casing has broken jar, four edges in the bottom of casing all have the supporting seat through.

As a further aspect of the present invention: the surface of the crushing ball is provided with uniformly distributed raised scribes, and the bottom end inside the crushing tank is in an inclined landslide shape.

As a further aspect of the present invention: the inner wall top-down of broken jar staggers in proper order and is provided with the striking board, the top movable mounting of broken jar has sealed lid, one side casing top fixed mounting of broken jar has the circulating pump, the output and the input of circulating pump are connected with the top and the bottom of broken jar through the pipeline respectively.

As a further aspect of the present invention: the power box is fixedly arranged on the rear side surface of the shell and is electrically connected with the motor and the circulating pump through a wire.

As a further aspect of the present invention: the rear side surface of the shell is fixedly provided with a control button, and the control button is electrically connected with the motor and the circulating pump through a wire.

As a further aspect of the present invention: the surface of the shell close to the front side is movably provided with a sealing plate through a hinge, and the surface of the sealing plate is fixedly provided with a handle.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the rotating rod and the stirring paddle are driven by the motor to rotate, so that the stirring paddle can be in contact with the crushing ball to push, the cell suspension and the crushing ball are mixed and stirred, the crushing ball and the raised scribing sheets on the surface of the crushing ball have the effects of high-acceleration impact, shearing, extrusion, cutting and the like on hard cells, an ideal crushing effect can be achieved in a very short time, materials are fluidized in the crushing process, each particle has the same stress state, the effect of precise mixing is achieved while crushing, and the crushing efficiency is improved;

2. can circulate derivation and input with the inside cell suspension of broken jar through the circulating pump, increase cell suspension at the inside flow velocity and the strength of flow of broken jar, at the high-speed in-process that flows, cell suspension produces the striking effect under the effect of striking plate for the cell in the cell suspension plays preliminary crushing effect, makes broken effect better.

Drawings

FIG. 1 is a front view of a cell disruption device.

FIG. 2 is a schematic view showing the internal structure of a cell disruption device.

FIG. 3 is a rear view of a cell disruption device.

Shown in the figure: 1. a sealing cover; 2. a crushing tank; 3. a circulation pump; 4. a housing; 5. a handle; 6. a motor; 7. a sealing plate; 8. a conical diversion cavity; 9. a discharge port; 10. a column; 11. a supporting seat; 12. a separation net; 13. a stirring paddle; 14. rotating the rod; 15. crushing a ball; 16. a flow guide port; 17. an impact plate; 18. a control valve; 19. a power box; 20. and a control button.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1 to 3, in an embodiment of the present invention, a cell crushing apparatus includes a housing 4, a rotating rod 14 is rotatably installed inside the housing 4 through a bearing, a stirring paddle 13 is uniformly installed on the surface of the rotating rod 14, a crushing ball 15 is installed inside the housing 4, a separation net 12 is fixedly installed at the bottom inside the housing 4, cells with qualified particle size can be filtered and separated in a crushing process, so that the cells are more purified, a conical diversion cavity 8 is fixedly installed below the separation net 12, a discharge port 9 is opened at the bottom of the conical diversion cavity 8, a motor 6 is installed on one side surface of the housing 4 through a fixing plate, an output end of the motor 6 penetrates through the surface of the housing 4 and is connected with one end of the rotating rod 14, a diversion port 16 is installed at the top of the housing 4, a control valve 18 is installed at both the diversion port 16 and the discharge port 9, a crushing tank, four edges of the bottom of the shell 4 are all provided with a supporting seat 11 through an upright post 10, the surface of the crushing ball 15 is provided with uniformly distributed convex scribing sheets, and the bottom end of the inner part of the crushing tank 2 is inclined to form a slope, so that the flow guide process is more rapid and smooth, and the cell suspension is prevented from accumulating at the bottom of the crushing tank 2.

The inner wall of the crushing tank 2 is sequentially provided with striking plates 17 in a staggered manner from top to bottom, the top of the crushing tank 2 is movably provided with a sealing cover 1, the top of a shell 4 at one side of the crushing tank 2 is fixedly provided with a circulating pump 3, the output end and the input end of the circulating pump 3 are respectively connected with the top and the bottom of the crushing tank 2 through pipelines, the rear side surface of the shell 4 is fixedly provided with a power box 19, the power box 19 is electrically connected with a motor 6 and the circulating pump 3 through wires, the rear side surface of the shell 4 is fixedly provided with a control button 20, the control button 20 is electrically connected with the motor 6 and the circulating pump 3 through wires, the coordinated operation can be conveniently carried out through a manual control device, the waste of energy is avoided, the sealing plate 7 is movably arranged at the front side surface of the shell 4 through a hinge, the handle 5 is fixedly arranged on the, avoid pollution and blockage when the material is crushed again.

The utility model discloses a theory of operation is: when cell crushing work is required, firstly, cell suspension liquid required to be crushed can be guided into the crushing tank 2, the cell suspension liquid in the crushing tank 2 is circularly guided out and input by starting the circulating pump 3, the flowing speed and the flowing strength of the cell suspension liquid in the crushing tank 2 are increased, in the high-speed flowing process, the cell suspension liquid generates an impact effect under the action of the impact plate 17, cells in the cell suspension liquid play a primary crushing effect, the control valve 18 is circularly opened for about 4-5 times, the primarily crushed suspension liquid enters the shell 4 through the flow guide port 16, then the rotating rod 14 and the stirring paddle 13 are driven to rotate by the motor 6, the stirring paddle 13 can be in contact with and push the crushing ball 15, so that the cell suspension liquid and the crushing ball 15 are mixed and stirred, and the hard cells are subjected to high-acceleration impact through the crushing ball 15 and the convex scratches on the surface of the crushing ball 15, Cut and rub, effect such as extrusion, cutting, can reach the crushing effect of ideal in the time of the utmost point short, the material is the fluidization at crushing in-process, makes every granule have the same stress state, reaches the effect of accurate mixture in the breakage, and the cell that reaches qualified particle diameter after the last breakage filters to toper water conservancy diversion chamber 8 inside through separation net 12 and derives the collection can.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.

Claims

1. A cell disruption device comprising a housing (4), characterized in that: the inboard of casing (4) is rotated through the bearing and is installed dwang (14), the surface of dwang (14) evenly is provided with stirring rake (13), the inboard of casing (4) is provided with broken ball (15), the inside bottom fixed mounting of casing (4) has separation net (12), the below fixed mounting of separation net (12) has toper water conservancy diversion chamber (8), discharge port (9) have been seted up to the bottom of toper water conservancy diversion chamber (8), motor (6) are installed through the fixed plate to a side surface of casing (4), the output of motor (6) runs through casing (4) surface and is connected with the one end of dwang (14), the top of casing (4) is provided with water conservancy diversion mouth (16), water conservancy diversion mouth (16) and discharge port (9) department all are provided with control valve (18), the top fixed mounting of casing (4) has broken jar (2), four corners of the bottom of the shell (4) are provided with supporting seats (11) through upright posts (10).

2. A cell disruption device according to claim 1, wherein: the surface of the crushing ball (15) is provided with uniformly distributed raised scribes, and the bottom end in the crushing tank (2) is in an inclined landslide shape.

3. A cell disruption device according to claim 1, wherein: the inner wall top-down of broken jar (2) is crisscross in proper order and is provided with striking plate (17), the top movable mounting of broken jar (2) has sealed lid (1), one side casing (4) top fixed mounting of broken jar (2) has circulating pump (3), the output and the input of circulating pump (3) are connected through the top and the bottom of pipeline with broken jar (2) respectively.

4. A cell disruption device according to claim 1 or claim 3, wherein: the rear side surface of the shell (4) is fixedly provided with a power box (19), and the power box (19) is electrically connected with the motor (6) and the circulating pump (3) through a lead.

5. A cell disruption device according to claim 1 or claim 3, wherein: the rear side surface of the shell (4) is fixedly provided with a control button (20), and the control button (20) is electrically connected with the motor (6) and the circulating pump (3) through a lead.

6. A cell disruption device according to claim 1, wherein: the side surface, close to the front side, of the shell (4) is movably provided with a sealing plate (7) through a hinge, and the surface of the sealing plate (7) is fixedly provided with a handle (5).