CN211317946U

CN211317946U - Immunomagnetic bead detection kit and immunomagnetic bead detection reagent mixing device

Info

Publication number: CN211317946U
Application number: CN201922168683.2U
Authority: CN
Inventors: 林斯
Original assignee: Tianjin Huaketai Biotechnology Co ltd
Current assignee: Tianjin Huaketai Biotechnology Co ltd
Priority date: 2019-12-06
Filing date: 2019-12-06
Publication date: 2020-08-21
Anticipated expiration: 2029-12-06

Abstract

The application relates to an immunomagnetic bead detect reagent box and immunomagnetic bead detect reagent mixing device, wherein, immunomagnetic bead detect reagent box includes: the magnetic particle cup is provided with a transmission part at the bottom end, and a cross structure or a three-fork structure is arranged in the transmission part; the cross structure or the three-fork structure is arranged to be circumferentially fixed with an output shaft of the electric motor; the reagent rack comprises a reagent rack body and a reagent rack upper cover; the reagent rack body is provided with a through hole for inserting the magnetic particle cup and one or more reagent cavities; the reagent rack upper cover is provided with openings corresponding to the one or more reagent cavities; wherein, the magnetic particle cup can be driven by the transmission part to rotate in the through hole, and the magnetic particle cup can be driven by the electric motor to rotate in the through hole. The immunomagnetic bead detection kit with various driving modes is realized.

Description

Immunomagnetic bead detection kit and immunomagnetic bead detection reagent mixing device

Technical Field

The application relates to the field of medical inspection, especially, relate to an immunomagnetic bead detection kit and immunomagnetic bead detection reagent mixing device.

Background

The immunomagnetic bead reagent is easy to precipitate, and needs to be in a uniform suspension state in the use process, so that the reagent needle can obtain uniform quantity when absorbing the reagent, the reaction quality is ensured, and the accuracy of the experimental result is facilitated. The existing reagent mixing method generally comprises the following steps: a manual shaking mode, a mechanical stirring mode, an ultrasonic stirring mode or a uniform mixing magnetic rod mode. The above-described manner of mixing reagents requires a long time and is not suitable for use in fully automated instruments.

Therefore, in order to facilitate the immunoassay, it is necessary to provide a rapid mixing immunomagnetic bead assay kit device, so that the immunomagnetic beads can be suspended at any time and rapidly.

In the related art, chinese patent application No. 2013101320483 discloses a reagent bottle, a reagent mixing device and a method. In this patent, reagent mixing device is including drive device and reagent bottle. The driving device comprises an installation mechanism and a transmission mechanism, and the installation mechanism can move relative to the transmission mechanism. Referring to the attached drawings of the patent, the driving device in the patent drives a plurality of reagent bottles simultaneously, and can only uniformly mix the plurality of reagent bottles. In addition, the transmission part in the patent is the insection arranged on the surface of the cylindrical cup wall, and the damage of the cylindrical cup wall provided with the insection can be caused by the use of the transmission part for a long time.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem or at least partially solve the technical problem, the application provides an immunomagnetic bead detection kit and an immunomagnetic bead detection reagent blending device.

In a first aspect, the present application provides an immunomagnetic bead assay kit, comprising: the bottom of the magnetic particle cup is provided with a cross structure or a trifurcate structure which can be fixed with the output shaft of the electric motor in the circumferential direction; the reagent rack comprises a reagent rack body and a reagent rack upper cover; the reagent rack body is provided with a through hole for inserting the magnetic particle cup and one or more reagent cavities; the reagent rack upper cover is provided with openings corresponding to the one or more reagent cavities; wherein, the magnetic particle cup can be driven by the electric motor to rotate in the through hole.

Through this immunomagnetic bead detect reagent box, adopt cross structure or trident structure and electric motor's output shaft circumference fixed, and then drive the rotation of magnetic particle cup by the circumferential direction of output shaft, can realize the purpose of quick mixing reagent, greatly practiced thrift the time of detection. The magnetic particle cups are driven by the electric motor to mix the reagent, and each magnetic particle cup can be independently mixed.

In some embodiments, the cross structure or the three-fork structure is disposed in a cylinder at the bottom end of the magnetic particle cup, a driving gear structure is disposed on the outer surface of the cylinder, and the magnetic particle cup can rotate in the through hole under the driving of the driving gear structure.

In some embodiments, the magnetic particle cup is provided with a stirring rib in a shape of a long strip, a square block, a spiral or a round block.

In some embodiments, the reagent rack body comprises a through hole part provided with a through hole, and a reagent cavity part provided with one or more reagent cavities, wherein the through hole part can be detachably connected with the reagent cavity part to form a whole; or the through hole of the reagent rack body and the one or more reagent cavities are integrally formed in an injection molding mode.

In some embodiments, one end of the bottom of the reagent rack body is provided with a limiting step which can be clamped into the limiting ring, and the other end of the bottom of the reagent rack body is provided with a downward extending elastic buckle which can be clamped into the limiting groove.

In a second aspect, the present application provides another immunomagnetic bead assay kit comprising: the magnetic particle cup is provided with a transmission part at the bottom end, and a cross structure or a three-fork structure is arranged in the transmission part; the cross structure or the three-fork structure is arranged to be circumferentially fixed with an output shaft of the electric motor; the reagent rack comprises a reagent rack body and a reagent rack upper cover; the reagent rack body is provided with a through hole for inserting the magnetic particle cup and one or more reagent cavities; the reagent rack upper cover is provided with openings corresponding to the one or more reagent cavities; wherein, the magnetic particle cup can be driven by the transmission part to rotate in the through hole, and the magnetic particle cup can be driven by the electric motor to rotate in the through hole.

Through this immunomagnetic bead detect reagent box, the bottom of magnetic particle cup is provided with transmission portion, can drive the magnetic particle cup rotatory through the drive arrangement in the correlation technique (the chinese patent that application number is 2013101320483), adopt cross structure or trident structure can be fixed with electric motor's output shaft circumference simultaneously, and then drive the rotation of magnetic particle cup by the circumferential direction of output shaft, still realized carrying out the reagent mixing by electric motor drive magnetic particle cup, can independently carry out the mixing to every magnetic particle cup. In addition, the transmission part is reinforced through the cross structure or the three-fork structure, and the damage to the transmission part is reduced.

In a third aspect, the present application provides an immunomagnetic bead detection reagent blending device, including: any of the above immunomagnetic bead detection kits; a drive device, comprising: the output shaft of the electric motor is provided with a claw structure which has a cross structure or a three-fork structure and can be inserted into the magnetic particle cup, so that the output shaft and the magnetic particle cup are circumferentially fixed; the magnetic particle cup can be driven by the electric motor to rotate in the through hole.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present application and together with the description, serve to explain the principles of the application.

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.

Fig. 1 is a schematic overall structure diagram of a uniform mixing device for an immunomagnetic bead detection reagent provided in an embodiment of the present application;

FIG. 2 is a schematic perspective view of the reagent kit of FIG. 1;

FIG. 3 is a schematic plan view of the magnetic particle cup of FIG. 2;

FIG. 4A is a schematic perspective view of the magnetic particle cup of FIG. 2;

FIG. 4B is a schematic perspective view of the magnetic particle cup of FIG. 2;

FIG. 5 is a schematic view of the structure of the bottom of the magnetic particle cup of FIG. 2;

FIG. 6 is a schematic cross-sectional view of the magnetic particle cup of FIG. 2;

FIG. 7 is a schematic view of the overall structure of the reagent rack body in FIG. 2;

FIG. 8 is a schematic structural view of the bottom of the reagent rack body in FIG. 2;

FIG. 9 is a schematic cross-sectional view of the reagent rack body in FIG. 2;

FIG. 10 is a schematic view of the overall structure of the upper cover of the reagent rack in FIG. 2;

FIG. 11 is a schematic view of the bottom of the upper cover of the reagent rack in FIG. 2;

FIG. 12 is a schematic structural view of the electric actuator of FIG. 1;

fig. 13 is a schematic structural view of the electric motor in fig. 12.

Detailed Description

It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.

In the following description, suffixes such as "module", "component", or "unit" used to denote elements are used only for the convenience of description of the present application, and have no specific meaning by themselves. Thus, "module", "component" or "unit" may be used mixedly.

Referring to fig. 1, an immunomagnetic bead detection reagent blending device according to an embodiment of the present application includes: a reagent kit 1 and a driving device 2. The cartridge 1 and the driving device 2 according to the embodiment of the present application will be further described with reference to fig. 2 to 13.

Referring to fig. 2-13, in certain embodiments, kit 1 comprises: a magnetic particle cup 3, the bottom of the magnetic particle cup 3 is provided with a cross structure 14 or a trifurcate structure (not shown in the figure) which can be fixed with the output shaft of the electric motor 29 in the circumferential direction; the reagent rack comprises a reagent rack body 4 and a reagent rack upper cover 5; wherein, the reagent rack body 4 is provided with a through hole 16 for inserting the magnetic particle cup 3 and one or more reagent cavities (shown as a first reagent cavity 17, a second reagent cavity 18 and a third reagent cavity 19 in fig. 7 and 9); the reagent rack upper cover 5 is provided with openings (shown as a first opening 25, a second opening 26, and a third opening 27 in fig. 10 and 11) corresponding to one or more reagent chambers. Wherein the magnetic particle cup 3 is rotatable in the through hole 16 by an electric motor 29. Through this embodiment, adopt cross structure 14 and electric motor 29's output shaft circumference fixed, and then drive the rotation of magnetic particle cup 3 by the circumferential direction of output shaft, can realize the purpose of quick mixing reagent, greatly practiced thrift the time of detecting. The magnetic particle cup 3 is driven by the electric motor 29 to mix the reagent, and each magnetic particle cup 3 can be independently mixed.

In some embodiments, as shown in fig. 3, 4A and 5, the crisscross structure 14 is disposed in a cylinder at the bottom end of the magnetic particle cup 3, the outer surface of the cylinder is provided with a driving gear structure 12, and the magnetic particle cup 3 can rotate in the through hole 16 under the driving of the driving gear structure 12. Through this embodiment, magnetic particle cup 3 can also be driven to rotate through the drive arrangement in the correlation technique (chinese patent application No. 2013101320483), and adopt criss-cross structure 14 and the output shaft circumference of electric motor 29 to fix simultaneously, and then drive the rotation of magnetic particle cup 3 by the circumferential direction of output shaft, realized carrying out the reagent mixing by electric motor 29 drive magnetic particle cup 3, can independently carry out the mixing to every magnetic particle cup 3. In addition, the transmission part (the transmission tooth structure 12) is reinforced by the crisscross structure 14, so that damage to the transmission part is reduced.

In some embodiments, the reagent rack body 4 comprises a through hole portion (not labeled) provided with the through hole 16, and a reagent cavity portion (not labeled) having one or more reagent cavities (shown as a first reagent cavity 17, a second reagent cavity 18, and a third reagent cavity 19 in fig. 7 and 9), wherein the through hole portion can be detachably connected with the reagent cavity portion to form a whole. In other embodiments, the through hole 16 and the one or more reagent cavities (shown in fig. 7, 9 as a first reagent cavity 17, a second reagent cavity 18 and a third reagent cavity 19) of the reagent rack body 4 are injection molded in one piece.

In some embodiments, referring to fig. 4B, at least one stirring rib 13 is disposed inside the magnetic particle cup 3, wherein the stirring rib 13 may be in the shape of a long strip, a square block, a spiral, or a round block. The stirring ribs 13 are used for generating turbulent flow under the rotation of the magnetic particle cup 3, so that the purpose of uniformly mixing the reagent is achieved.

In some embodiments, the reagent rack body 4 and the reagent rack upper cover 5 are integrally connected by welding, bonding, adhering or elastomer sealing, and further welding may be ultrasonic welding.

In some embodiments, the reagent rack upper cover 5 is provided with a circular, square, triangular or rhombic opening corresponding to each reagent cavity. Fig. 2, 10, and 11 illustrate the opening as a circle, but the embodiment of the present application is not limited thereto.

Although fig. 7 and 9 illustrate the through hole 16 penetrating from top to bottom and having a closed ring shape, in some embodiments, the through hole 16 may have a non-closed ring shape or be formed by two upper and lower rings, which is not limited in this embodiment.

In some embodiments, as shown in fig. 8, the bottom of the reagent rack body 4 is provided with a positioning hole 23 for fixing the reagent cartridge 1, for example, to the driving device in chinese patent No. 2013101320483. The stability of the kit in a detection instrument is improved, and the operation is convenient.

In some embodiments, as shown in fig. 9, the reagent rack body 4 has a resilient clip 21 extending downward from a side close to the reagent chamber, and a limiting step 22 is provided at a bottom of a side close to the through hole 16. In the market, an inclined elastic buckle (for example, chinese patent No. 2013101320483) is generally arranged at the bottom of a reagent rack body, and the unhooking phenomenon is easily generated during the process of pushing and pulling a reagent kit. Through this embodiment, the reagent frame body has set up the elasticity buckle near one side downwardly extending of reagent cavity, has solved the unhook problem that exists at the in-process of push-and-pull kit.

In some embodiments, as shown in fig. 7, the reagent rack body 4 is provided with anti-slip parts 20 at two sides of one end close to the reagent cavity, so as to increase friction and facilitate a user to grasp a reagent box. By way of non-limiting example, as shown in FIG. 8, the cleats 20 may be semi-circular, square, pyramidal, or prismatic arrays of protrusions.

In certain embodiments, referring to fig. 3, the magnetic particle cup 3 comprises a first cylinder 8, a second cylinder 9, a third cylinder 10, a fourth cylinder 11, and a transmission. Referring to fig. 4A and 5, the transmission part is disposed at the lower end of the fourth cylinder 11 of the magnetic particle cup, the outer side of the transmission part is provided with a transmission gear structure 12, the transmission gear structure is a circular gear surrounding a circle, the outer diameter of the gear is smaller than the inner diameter of the through hole, and the inner side of the transmission part is provided with a cross structure 14.

With further reference to fig. 3 and 9, the outer diameter of the first cylinder 8 is larger than the inner diameter of the through hole 16 of the reagent rack body 4 for serving as a support surface for the magnetic particle cup when rotating in the through hole 16; the outer diameter of the second cylinder 9 is smaller than the inner diameter of the through hole 16 and larger than the outer diameter of the third cylinder 10, and is used as a supporting surface contacted with the inner wall of the through hole 16 when the magnetic particle cup 3 rotates; the third cylinder 10 is not in contact with the inner surface of the through hole 16, so that the contact area between the magnetic particle cup 3 and the inner surface of the through hole 16 is reduced, the friction force is reduced, and the magnetic bead liquid can be more efficiently and uniformly mixed. The sum of the lengths of the second cylinder 9 and the third cylinder 10 is greater than the length of the through hole 16; the outer diameter of the fourth cylinder 11 is smaller than the inner diameter of the through hole 16 and larger than the outer diameter of the third cylinder 10; the outer diameter of the gear tooth formation 12 is smaller than the inner diameter of the through bore 16.

In some embodiments, as shown with reference to fig. 4B and 10, the magnetic particle cup 3 and the reagent rack cover 5 are provided with annular steps 24a to 24d on the inside.

In certain embodiments, and as shown with reference to FIG. 2, the annular steps 24 a-24 d are provided with the puncture membranes 6 a-6 d. The middle of the puncture films 6a to 6d is of a herringbone or cross-shaped crack structure, so that a reagent needle can conveniently suck a reagent. The upper ends of the bottle mouths of the magnetic particle cups 3 and the reagent rack upper cover 4 are heat-sealed with easy-to-

tear films

7a and 7 b.

Referring to fig. 12 and 13, the driving device 2 includes a base plate 28 and an electric motor 29, the electric motor 29 is fixed on the base plate 28, a driving claw 30 is provided on the top of the electric motor 29, and the driving claw 30 is provided as four claws or three claws (not shown) according to the structure of the magnetic particle cup 3. When the magnetic particle cup is in operation, the transmission claw 30 is correspondingly inserted into the cross structure 14 or the three-fork structure of the magnetic particle cup, and then the electric motor 29 drives the magnetic particle cup 3 to rotate at high speed, so as to achieve the purpose of rapid mixing.

It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.

The above-mentioned serial numbers of the embodiments of the present application are merely for description and do not represent the merits of the embodiments.

While the present embodiments have been described with reference to the accompanying drawings, it is to be understood that the invention is not limited to the precise embodiments described above, which are meant to be illustrative and not restrictive, and that various changes may be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.

Claims

1. An immunomagnetic bead detection kit, comprising:

the bottom of the magnetic particle cup is provided with a cross structure or a trifurcate structure which can be circumferentially fixed with an output shaft of the electric motor;

the reagent rack comprises a reagent rack body and a reagent rack upper cover; the reagent rack body is provided with a through hole for inserting the magnetic particle cup and one or more reagent cavities; the reagent rack upper cover is provided with openings corresponding to the one or more reagent cavities;

wherein the magnetic particle cup can be driven by the electric motor to rotate in the through hole.

2. The immunomagnetic bead detection kit of claim 1, wherein the cross-shaped structure or the three-forked structure is disposed in a cylinder at the bottom end of the magnetic particle cup, and the outer surface of the cylinder is provided with a driving tooth structure, and the magnetic particle cup can rotate in the through hole under the driving of the driving tooth structure.

3. The immunomagnetic bead detection kit of claim 1, wherein the magnetic particle cup is provided with a stirring rib in a shape of a strip, a square, a spiral or a round block.

4. The immunomagnetic bead test kit of claim 1, wherein the reagent rack body comprises: the reagent chamber comprises a through hole part provided with the through hole and a reagent chamber part provided with one or more reagent chambers, wherein the through hole part can be detachably connected with the reagent chamber part to form a whole; or the through hole of the reagent rack body and the one or more reagent cavities are integrally formed in an injection molding mode.

5. The immunomagnetic bead detection kit of claim 1 or 2, wherein one end of the bottom of the reagent rack body is provided with a limiting step capable of being clamped into the limiting ring, and the other end of the bottom of the reagent rack body is provided with a downwardly extending elastic buckle capable of being clamped into the limiting groove.

6. An immunomagnetic bead detection kit, comprising:

the magnetic particle cup is characterized in that the bottom end of the magnetic particle cup is provided with a transmission part, and a cross structure or a three-fork structure is arranged in the transmission part; the cross structure or the three-fork structure is arranged to be circumferentially fixed with an output shaft of the electric motor;

wherein the magnetic particle cup can rotate in the through hole under the driving of the transmission part, and the magnetic particle cup can rotate in the through hole under the driving of the electric motor.

7. The immunomagnetic bead detection kit of claim 6, wherein the magnetic particle cup is provided with a stirring rib in a shape of a strip, a square, a spiral or a round block.

8. The immunomagnetic bead test kit of claim 6, wherein the reagent rack body comprises: the reagent chamber comprises a through hole part provided with the through hole and a reagent chamber part provided with one or more reagent chambers, wherein the through hole part can be detachably connected with the reagent chamber part to form a whole; or the through hole of the reagent rack body and the one or more reagent cavities are integrally formed in an injection molding mode.

9. The immunomagnetic bead detection kit of claim 6, wherein the bottom of the reagent rack body is provided with a limiting step capable of being clamped into the limiting ring at one end, and the bottom of the reagent rack body is provided with a downwardly extending elastic buckle capable of being clamped into the limiting groove at the other end.

10. The utility model provides an immunomagnetic bead detection reagent mixing device which characterized in that includes:

the immunomagnetic bead detection kit of any one of claims 1-9;

a drive device, comprising: an electric motor having an output shaft provided with a claw structure of a cross structure or a trifurcate structure into which the magnetic particle cup can be inserted, so that the output shaft and the magnetic particle cup are circumferentially fixed; the magnetic particle cup can be driven by the electric motor to rotate in the through hole.