CN211292706U - Biosensor for measuring dissolved oxygen at different depths of water body - Google Patents

Abstract

The utility model discloses a biosensor for measuring dissolved oxygen at different depths in a water body, comprising an anode connected by wires, the anode is arranged in a base filled with sediment; the base is closed by a cover plate arranged on the upper end face Sediment, two sides of the top surface of the base are respectively provided with telescopic rods; the upper ends of the telescopic rods are oppositely provided with connecting rods; a cathode is fixed between the connecting rods, and one end of the cathode is connected to the telescopic rod through a wire The resistance in the rod is connected, and the other end of the resistance is connected with the anode in the base through the wire; the other end of the cathode is connected with the pulling wire; Support rods and exit from the top of the support rods. The utility model aims to provide a biosensor based on a microbial fuel cell, which can conveniently measure the dissolved oxygen content at different depths in a water body by adjusting the distance between the cathode and the anode.

Description

A biosensor for measuring dissolved oxygen at different depths in water bodies

technical field

The utility model relates to the field of biological sensors, in particular to a biological sensor for measuring dissolved oxygen at different depths in a water body.

Background technique

Traditional dissolved oxygen measurement methods mainly include physical, chemical and electrochemical methods, etc. A variety of dissolved oxygen sensors have been developed, such as fluorescent dissolved oxygen sensors, which are widely used in the measurement of dissolved oxygen concentration. However, these electrode-type sensors have several disadvantages, such as relatively expensive electrode materials, difficulty in miniaturization, and electromagnetic interference from other sensors. A microbial fuel cell is a bioelectrochemical device that can directly convert chemical energy into electrical energy through a biological process catalyzed by exogenous microorganisms. Microbial fuel cells and their derived technologies have received increasing attention because of their ability to not only recover energy from wastewater and treat pollutants in the environment, but also serve as biosensors for environmental monitoring and are particularly promising for in situ and Self-powered sensing device for online environmental monitoring. Microbial fuel cell sensors utilize electroactive microorganisms as probes, and the presence or change of a target analyte affects the electron transfer process of the microorganisms, thereby generating an electrical signal. Since the voltage can be easily monitored online, microbial fuel cells can be used as cheap and reliable online biosensors. Therefore, there is an urgent need for a biosensor based on microbial fuel cells, which can easily measure the dissolved oxygen content at different depths in water by adjusting the distance between the cathode and anode.

Utility model content

The purpose of the utility model is to overcome the above-mentioned deficiencies in the prior art, and to provide a biosensor based on a microbial fuel cell, which can conveniently measure the dissolved oxygen content at different depths in a water body by adjusting the distance between the cathode and the anode.

In order to achieve the above object, the technical scheme of the present utility model is achieved in this way:

A biosensor for measuring dissolved oxygen at different depths in a water body, comprising an anode connected by wires, the anode is arranged in a pedestal filled with sediment; Two sides of the top surface of the base are respectively provided with telescopic rods; the upper ends of the telescopic rods are oppositely provided with connecting rods; cathodes are fixed between the connecting rods, and one end of the cathodes is connected to the resistance provided in the telescopic rods through wires. The other end of the resistor is connected to the anode in the base through a wire; the other end of the cathode is connected to the pulling wire; The top of the support rod is exported.

Further, the telescopic rod is composed of five hollow stainless steel tubes connected together, and each stainless steel tube is 20 cm long.

Further, the traction wire includes an inner wire layer and an outer insulating protective layer, and the insulating protective layer is made of PVC or PE.

Further, the surface area ratio of the anode and the cathode is 11:1, and both the anode and the cathode are composed of carbon felt or graphite plate.

Further, the cathode is cylindrical with a diameter of 8-12 cm and a thickness of 0.5-2 cm.

Further, the resistance value of the resistor is 1000Ω.

Further, the connecting rod is made of PVC plastic steel.

Compared with the prior art, the utility model has the following beneficial effects:

The utility model composes a microbial fuel cell sensor through the anode embedded in the anaerobic sediment and the cathode suspended in the aerobic water column above the sediment. Anaerobic microorganisms in the sediment can coat the anode, grow to form biofilms and repair themselves, significantly improving the stability and sustainability of the sensor, thereby directly generating and outputting electrical signals without additional power supply, simplifying the microbial Management and maintenance of fuel cell sensors and associated cost reductions. Compared with other types of biosensors, the utility model has the main advantages of being able to perform real-time monitoring through the electrical measuring element and being easy to carry. Dissolved oxygen in water is reduced to water at the cathode through a redox reaction, and the number of electrons transferred by microorganisms to the anode corresponds to the number of electrons that react with oxygen at the cathode. Thus by measuring the voltage developed at the cathode as a measure of the dissolved oxygen concentration.

Description of drawings

Fig. 1 is the structural representation of the utility model;

FIG. 2 is a schematic view of the structure of the traction wire in the utility model.

Description of reference numbers:

1-Pull wire, 2-Support rod, 3-Cathode, 4-Connecting rod, 5-Telescopic rod, 6-Resistor, 7-Wire, 8-Anode, 9-Base, 10-Cover, 101-Wire layer , 102-insulation protection layer.

Detailed ways

The present utility model will be described in detail below with reference to the accompanying drawings and in conjunction with the embodiments.

As shown in Figures 1 and 2, a biosensor for measuring dissolved oxygen at different depths in a water body includes an anode 8 connected by a wire 7, and the anode 8 is arranged in a base 9 filled with sediment; the base 9 The sediment is sealed by a cover plate 10 arranged on the upper end surface, and telescopic rods 5 are respectively provided on both sides of the top surface of the base 9; A hollow stainless steel tube connected together is formed, and each stainless steel tube is 20cm long; a cathode 3 is fixed between the connecting rods 4, and one end of the cathode 3 is connected with the resistance 6 arranged in the telescopic rod 5 through a wire 7 , the other end of the resistor 6 passes through the pre-opening hole on the base 9 through the wire 7, and is connected with the anode 8 in the base 9; the other end of the cathode 3 is connected with the pulling wire 1; the pulling wire The other end of 1 penetrates the support rod 2 provided on the upper end of the telescopic rod 5, and is led out from the top of the support rod 2. The traction wire 1 includes an inner wire layer 101 and an outer insulation protection layer 102. The insulation protection The layer 102 is composed of PVC or PE; the surface area ratio of the anode 8 to the cathode 3 is 11:1, and the anode 8 and the cathode 3 are both composed of carbon felt or graphite plate; the cathode 3 is cylindrical with a diameter of 8 -12cm, the thickness is 0.5-2cm; the resistance value of the resistor 6 is 1000 Ω; the connecting rod 4 is made of PVC plastic steel.

The utility model can measure water bodies with different depths between 20cm and 100cm. Before use, in order to ensure that there is enough organic matter in the sediment for anaerobic microorganism consumption, the sediment containing 5% organic matter is put into the base 9 in advance. middle. When using, first determine the depth of the water body to be measured, and adjust the lengths of the two telescopic rods 5 according to the distance between the measured depth and the bottom of the water body. The telescopic rods 5 in this embodiment can use the same telescopic rods on the umbrella. way to connect. Then put the sensor into the water body through the pulling wire 1, after the sensor sinks into the bottom of the water body, let the cathode 3 be at the water level of the required measurement depth, and then connect the pulling wire 1 to the electricity measuring device. The anode 8 provides a growth carrier for anaerobic microorganisms, and the anaerobic microorganisms will cover the anode 8 to form a biofilm, thereby generating a stable voltage. The dissolved oxygen in the water is reduced to water by a redox reaction at the cathode 3, and the number of electrons transferred by the anaerobic microorganisms to the anode 8 corresponds to the number of electrons that the cathode 3 reacts with oxygen. When the surface area ratio of the anode 8 to the cathode 3 is 11:1, the performance of the sensor is optimal, so by measuring the voltage generated by the cathode 3, the dissolved oxygen content of the water body at the depth of the water body can be converted and calculated. If you need to measure the dissolved oxygen content at other depths, take the sensor out of the water body through the traction line 1, then change the length of the telescopic rod 5 until it meets the requirements, and then put the sensor into the water body to collect the voltage.

"Development of a Biosensor for Simultaneous Online Monitoring of Dissolved Oxygen Concentrations at Different Depths in Lake Water Based on Sedimentary Microbial Fuel Cells" by Song Na, Yan Zaisheng, Xu Huacheng, Yao Zongbao, Wang Changhui, Chen Mo, Zhao Zhiwei, Peng Zhaoliang, Wang Chunliu, He Longjiang, p. 272 ~ 280 pages, Total Environmental Science, January 2019. The experimental data in this article shows that the voltage output has a linear relationship with the dissolved oxygen concentration. By analyzing and processing the experimental data, the linear relationship between the voltage and dissolved oxygen can be obtained as: y=0.014x-0.123 (x is the voltage, y is the dissolved oxygen ). The voltage output by the sensor is brought into the above relationship, and the dissolved oxygen content in the water body can be calculated.

Wherein, the pulling wire 1 includes an inner wire layer 101 and an outer insulating protective layer 102, and the insulating protective layer 102 is composed of PVC or PE. The wire layer in the traction wire can transmit electrical signals, and the insulating protective layer of the outer layer has a certain tensile strength, which can protect the inner wire layer, and at the same time, the traction wire has the strength to drop the sensor into the water body and recover it.

Wherein, the connecting rod 4 is made of PVC plastic steel. PVC plastic steel has the advantages of good insulation, high strength, corrosion resistance and light weight, and it can play a good insulating role when fixing the cathode.

The above are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the within the scope of protection of the present invention.

Claims (7)

1. The utility model provides a survey different degree of depth dissolved oxygen's of water biosensor, includes the positive pole through wire connection, its characterized in that: the anode is arranged in the base filled with the sediment; the base seals the sediment through a cover plate arranged on the upper end surface, and telescopic rods are respectively arranged on two sides of the top surface of the base; the upper end part of the telescopic rod is oppositely provided with a connecting rod; a cathode is fixed between the connecting rods, one end of the cathode is connected with a resistor arranged in the telescopic rod through a lead, and the other end of the resistor is connected with an anode in the base through a lead; the other end of the cathode is connected with a traction wire; the other end of the traction wire penetrates through the supporting rod arranged at the upper end part of the telescopic rod and is led out from the top of the supporting rod.

2. The biosensor for measuring dissolved oxygen at different depths in a water body according to claim 1, wherein: the telescopic link comprises five hollow stainless steel pipes that link together, and every stainless steel pipe length is 20 cm.

3. The biosensor for measuring dissolved oxygen at different depths in a water body according to claim 1, wherein: the traction wire comprises an inner wire layer and an outer insulating protective layer, wherein the insulating protective layer is made of PVC or PE.

4. The biosensor for measuring dissolved oxygen at different depths in a water body according to claim 1, wherein: the surface area ratio of the anode to the cathode is 11: 1, and the anode and the cathode are both composed of carbon felt or graphite plates.

5. The biosensor for measuring dissolved oxygen at different depths in a water body according to claim 1, wherein: the cathode is cylindrical, the diameter of the cathode is 8-12cm, and the thickness of the cathode is 0.5-2 cm.

6. The biosensor for measuring dissolved oxygen at different depths in a water body according to claim 1, wherein: the resistance value of the resistor is 1000 omega.

7. The biosensor for measuring dissolved oxygen at different depths in a water body according to claim 1, wherein: the connecting rod is made of PVC plastic steel.

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