CN211292706U

CN211292706U - Biosensor for measuring dissolved oxygen at different depths of water body

Info

Publication number: CN211292706U
Application number: CN201921355458.3U
Authority: CN
Inventors: 吴卿; 焦士埔; 肖京京; 彭森
Original assignee: Tianjin University
Current assignee: Tianjin University
Priority date: 2019-08-20
Filing date: 2019-08-20
Publication date: 2020-08-18
Anticipated expiration: 2029-08-20

Abstract

The utility model discloses a biosensor for measuring dissolved oxygen at different depths in a water body, which comprises an anode connected by a lead, wherein the anode is arranged in a base filled with sediments; the base seals the sediment through a cover plate arranged on the upper end surface, and telescopic rods are respectively arranged on two sides of the top surface of the base; the upper end part of the telescopic rod is oppositely provided with a connecting rod; a cathode is fixed between the connecting rods, one end of the cathode is connected with a resistor arranged in the telescopic rod through a lead, and the other end of the resistor is connected with an anode in the base through a lead; the other end of the cathode is connected with a traction wire; the other end of the traction wire penetrates through the supporting rod arranged at the upper end part of the telescopic rod and is led out from the top of the supporting rod. The utility model aims at providing a take microbial fuel cell as the basis, through adjusting the distance of negative pole, positive pole, can survey the biosensor of the different degree of depth dissolved oxygen content of water very conveniently.

Description

Biosensor for measuring dissolved oxygen at different depths of water body

Technical Field

The utility model relates to a biosensor field especially relates to a survey water different degree of depth dissolved oxygen's biosensor.

Background

The conventional dissolved oxygen measurement methods mainly include physical, chemical, and electrochemical methods, and various dissolved oxygen sensors, such as fluorescent dissolved oxygen sensors, have been developed and widely used for measuring the concentration of dissolved oxygen. However, these electrode type sensors have several disadvantages, such as the relatively expensive electrode materials, difficulty in miniaturization, and electromagnetic interference from other sensors. A microbial fuel cell is a bioelectrochemical device that can directly convert chemical energy into electrical energy through a biological process catalyzed by an exogenous microorganism. Microbial fuel cells and their derived technologies are receiving increasing attention because they not only recover energy from wastewater and treat pollutants in the environment, but also serve as biosensors to monitor the environment, and are particularly promising as self-powered sensing devices for in-situ and online environmental monitoring. Microbial fuel cell sensors utilize an electroactive microbe as a probe, and the presence or change of a target analyte can affect the electron transfer process of the microbe, thereby generating an electrical signal. Since the voltage can be easily monitored on-line, the microbial fuel cell can be used as an inexpensive, reliable on-line biosensor. Therefore, a biosensor based on a microbial fuel cell is urgently needed, and the dissolved oxygen content of water bodies at different depths can be conveniently measured by adjusting the distance between a cathode and an anode.

SUMMERY OF THE UTILITY MODEL

The utility model aims to overcome the defects in the prior art, and aims to provide a biosensor which is based on a microbial fuel cell and can conveniently measure the dissolved oxygen content of different depths of water bodies by adjusting the distance between a cathode and an anode.

In order to achieve the above purpose, the technical scheme of the utility model is realized like this:

a biosensor for measuring dissolved oxygen at different depths in a water body comprises an anode connected through a lead, wherein the anode is arranged in a base filled with sediment; the base seals the sediment through a cover plate arranged on the upper end surface, and telescopic rods are respectively arranged on two sides of the top surface of the base; the upper end part of the telescopic rod is oppositely provided with a connecting rod; a cathode is fixed between the connecting rods, one end of the cathode is connected with a resistor arranged in the telescopic rod through a lead, and the other end of the resistor is connected with an anode in the base through a lead; the other end of the cathode is connected with a traction wire; the other end of the traction wire penetrates through the supporting rod arranged at the upper end part of the telescopic rod and is led out from the top of the supporting rod.

Furthermore, the telescopic rod is composed of five hollow stainless steel pipes which are connected together, and each stainless steel pipe is 20cm long.

Further, the traction wire comprises an inner wire layer and an outer insulating protective layer, wherein the insulating protective layer is made of PVC or PE.

Further, the surface area ratio of the anode to the cathode is 11: 1, and the anode and the cathode are both made of carbon felt or graphite plates.

Furthermore, the cathode is cylindrical, the diameter of the cathode is 8-12cm, and the thickness of the cathode is 0.5-2 cm.

Further, the resistance value of the resistor is 1000 Ω.

Furthermore, the connecting rod is made of PVC plastic steel.

Compared with the prior art, the utility model discloses following beneficial effect has:

the utility model discloses an embedding positive pole in the anaerobism deposit and the suspension negative pole in the good oxygen water column in deposit top constitute microbial fuel cell sensor. Anaerobic microorganisms in the sediment can cover the anode, grow to form a biological film and repair the biological film by self, so that the stability and the sustainability of the sensor are obviously improved, an electric signal is directly generated and output, an additional power supply is not needed, the management and the maintenance of the microbial fuel cell sensor are simplified, and the related cost is reduced. Compared with other types of biosensors, the utility model has the main advantages that the accessible is surveyed the electric piece and is carried out real time monitoring, and portable. The dissolved oxygen in the water is reduced to water at the cathode by an oxidation-reduction reaction, and the number of electrons transferred to the anode by the microorganisms corresponds to the number of electrons reacted with oxygen at the cathode. And thus by measuring the voltage generated by the cathode as a measure of the dissolved oxygen concentration.

Drawings

Fig. 1 is a schematic structural view of the present invention;

fig. 2 is a schematic structural view of the middle pulling wire of the present invention.

Description of reference numerals:

1-a traction wire, 2-a support rod, 3-a cathode, 4-a connecting rod, 5-a telescopic rod, 6-a resistor, 7-a lead, 8-an anode, 9-a base, 10-a cover plate, 101-a lead layer and 102-an insulating protective layer.

Detailed Description

The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.

As shown in fig. 1 and 2, a biosensor for measuring dissolved oxygen at different depths in a water body comprises an anode 8 connected by a lead 7, wherein the anode 8 is arranged in a base 9 filled with sediment; the base 9 seals the sediment through a cover plate 10 arranged on the upper end surface, and the two sides of the top surface of the base 9 are respectively provided with a telescopic rod 5; the upper end of the telescopic rod 5 is relatively provided with a connecting rod 4, the telescopic rod 5 consists of five hollow stainless steel tubes which are connected together, and each stainless steel tube is 20cm long; a cathode 3 is fixed between the connecting rods 4, one end of the cathode 3 is connected with a resistor 6 arranged in the telescopic rod 5 through a lead 7, and the other end of the resistor 6 penetrates through a pre-opening hole in the base 9 through the lead 7 and is connected with an anode 8 in the base 9; the other end of the cathode 3 is connected with a traction wire 1; the other end of the traction wire 1 penetrates into a supporting rod 2 arranged at the upper end of the telescopic rod 5 and is led out from the top of the supporting rod 2, the traction wire 1 comprises an inner wire layer 101 and an outer insulating protective layer 102, and the insulating protective layer 102 is made of PVC or PE; the surface area ratio of the anode 8 to the cathode 3 is 11: 1, and both the anode 8 and the cathode 3 are made of carbon felt or graphite plates; the cathode 3 is cylindrical, the diameter is 8-12cm, and the thickness is 0.5-2 cm; the resistance value of the resistor 6 is 1000 omega; the connecting rod 4 is made of PVC plastic steel.

The utility model discloses can be to the degree of depth be the water of the different degree of depth between 20cm-100cm measure, for having sufficient organic matter in guaranteeing the deposit before the use and supply anaerobic microorganism to consume, put into base 9 with the deposit that contains 5% organic matter in advance. When the telescopic umbrella is used, firstly, the depth of the water body to be measured is determined, and the lengths of the two telescopic rods 5 are correspondingly adjusted according to the distance between the measured depth and the bottom surface of the water body. Then throw the sensor into the water through pull wire 1, make the sensor sink into the water bottom surface after, let negative pole 3 be located the water level of required survey degree of depth, again with pull wire 1 with survey the electric component and be connected. The anode 8 provides a carrier for growth of anaerobic microorganisms, which can cover the anode 8 by themselves to form a biofilm, thereby generating a stable voltage. Dissolved oxygen in the water is reduced to water at the cathode 3 by an oxidation-reduction reaction, and the number of electrons transferred to the anode 8 by the anaerobic microorganisms corresponds to the number of electrons reacted with oxygen at the cathode 3. When the surface area ratio of the anode 8 to the cathode 3 is 11: 1, the performance of the sensor is optimal, so that the dissolved oxygen content of the deep water body is calculated by measuring the voltage generated by the cathode 3 and performing conversion. If the content of dissolved oxygen of other degree of depth of needs survey, take out the sensor from the water through pull wire 1, then change the length of telescopic link 5 until meeting the requirements, it can to gather the voltage in the water to put in the sensor again.

The authors of the "development of a biosensor based on a sedimentary microbial fuel cell and simultaneously monitoring the dissolved oxygen concentrations at different depths in lake water on line" were sonna, yansheng, xuhuacheng, yao dongbao, wangzheng, chenmei, zhao shiwei, pengmara, wangchun, jorongjiang, pages 272-280, total environmental science, 1 month in 2019. The experimental data of the article show that the voltage output and the dissolved oxygen concentration are in a linear relationship, and the linear relationship between the voltage and the dissolved oxygen can be obtained by analyzing and processing the experimental data as follows: y is 0.014x-0.123(x is voltage and y is dissolved oxygen). And substituting the voltage output by the sensor into the relational expression, and calculating the content of the dissolved oxygen in the water body.

The traction wire 1 comprises an inner wire layer 101 and an outer insulating protective layer 102, wherein the insulating protective layer 102 is made of PVC or PE. The wire layer in the pull wire can carry out the transmission of signal of telecommunication, and outer insulating protective layer has certain tensile strength, can protect inside wire layer, lets the pull wire have simultaneously with the sensor to the intensity of throwing in and retrieving in the water.

Wherein, the connecting rod 4 is made of PVC plastic steel. The PVC plastic steel has the advantages of good insulation, high strength, corrosion resistance and light weight, and can play a good insulation role while fixing the cathode.

The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides a survey different degree of depth dissolved oxygen's of water biosensor, includes the positive pole through wire connection, its characterized in that: the anode is arranged in the base filled with the sediment; the base seals the sediment through a cover plate arranged on the upper end surface, and telescopic rods are respectively arranged on two sides of the top surface of the base; the upper end part of the telescopic rod is oppositely provided with a connecting rod; a cathode is fixed between the connecting rods, one end of the cathode is connected with a resistor arranged in the telescopic rod through a lead, and the other end of the resistor is connected with an anode in the base through a lead; the other end of the cathode is connected with a traction wire; the other end of the traction wire penetrates through the supporting rod arranged at the upper end part of the telescopic rod and is led out from the top of the supporting rod.

2. The biosensor for measuring dissolved oxygen at different depths in a water body according to claim 1, wherein: the telescopic link comprises five hollow stainless steel pipes that link together, and every stainless steel pipe length is 20 cm.

3. The biosensor for measuring dissolved oxygen at different depths in a water body according to claim 1, wherein: the traction wire comprises an inner wire layer and an outer insulating protective layer, wherein the insulating protective layer is made of PVC or PE.

4. The biosensor for measuring dissolved oxygen at different depths in a water body according to claim 1, wherein: the surface area ratio of the anode to the cathode is 11: 1, and the anode and the cathode are both composed of carbon felt or graphite plates.

5. The biosensor for measuring dissolved oxygen at different depths in a water body according to claim 1, wherein: the cathode is cylindrical, the diameter of the cathode is 8-12cm, and the thickness of the cathode is 0.5-2 cm.

6. The biosensor for measuring dissolved oxygen at different depths in a water body according to claim 1, wherein: the resistance value of the resistor is 1000 omega.

7. The biosensor for measuring dissolved oxygen at different depths in a water body according to claim 1, wherein: the connecting rod is made of PVC plastic steel.