CN211255915U - PCR tube - Google Patents

Abstract

The utility model relates to a PCR tube, which comprises a tube body and a cover body for covering the opening of the tube body, wherein the cover body comprises a shielding part for shielding the opening of the tube body and an annular clamping part connected with the shielding part; the lid is when covering, only the internal surface of occlusion part has the possibility of contacting this internal liquid of pipe, the annular joint portion of lid all contactless sample, can not cause the pollution, and simultaneously, in PCR reaction process, the hot lid of PCR appearance is pressed in PCR pipe top, the lid is compressed tightly very easily, with the pipe body on along in close contact with, can not leak gas, improve the gas tightness, improve the detectivity, avoid adopting liquid dropping paraffin and the paraffin interference problem that causes, improve operation personnel's operating efficiency.

Description

PCR tube

Technical Field

The utility model relates to a PCR pipe.

Background

In biological, medical and pharmaceutical experiments and forensic identification, PCR is one of the most common and important nucleic acid detection and identification techniques. The PCR reaction is usually carried out in a PCR tube or a PCR plate. PCR tubes of 200. mu.l capacity are commonly used, with single tubes, 8-tubes, 12-tubes, etc. The PCR tube structurally comprises a tube body and a cover. The cover is provided with a cylindrical protrusion which is clamped into the tube orifice so as to cover the tube body, for example, a PCR tube disclosed by Chinese patent CN106967592A, a high-speed PCR tube disclosed by Chinese patent CN201981185U, a PCR reaction tube disclosed by Chinese patent CN207062279U and the like belong to the structure, and the structure has several problems: if the contact between the cover and the tube body is not very tight (such as insufficient machining precision of the tube, deformation of the tube body or the cover, misoperation), air leakage is easy to occur. When PCR is carried out, a large amount of water vapor leaks out due to the high temperature of the reaction, so that the reaction system is changed, and the detection fails.

Also have and fill in the internal portion's of mouth of pipe external diameter through increasing the lid, so that the body and with increase the laminating dynamics between the lid, make the two more inseparable, but do so and can make and cover the lid or open the lid when, the operating personnel need use great strength just can open or cover, it is very loaded down with trivial details in the operation, though it has solved the problem of gas tightness to a certain extent, but this kind of mode that covers, cylindrical protrusion on the lid can be with the sample contact when the pipe is covered, so need avoid polluting this cylindrical protrusion, this cylindrical protrusion is touched into very easily to the hand when opening the tube cap that will close. During the experimental operation, the cylindrical protrusion on the tube cover is easily touched and polluted if the cover is in an open state.

In order to solve the above problems, the classic PCR experiment procedure is to add a drop of liquid paraffin on the reaction system, and cover the liquid to avoid evaporation of water. However, this solution adds an operation step on the one hand and on the other hand is likely to interfere with the detection if there are impurities in the liquid paraffin; moreover, if the PCR product is needed to be used for experiments such as electrophoresis or enzyme digestion, the PCR product is sucked out of the tube, some liquid paraffin is brought out inevitably, subsequent experiments are possibly interfered, and no better solution is provided at present aiming at the problem of paraffin interference detection, and only more attention can be paid in the operation.

Disclosure of Invention

The utility model aims to solve the technical problem that the prior art is not enough to be overcome, and a PCR tube is provided.

In order to solve the technical problem, the utility model discloses the technical scheme who takes as follows:

the utility model provides a PCR pipe, PCR pipe include the pipe body and be used for the lid to close the lid of pipe body opening, and the lid includes shielding part, the annular joint portion of connection on shielding part that the pipe body opening sheltered from, when the lid closes on the pipe body, annular joint portion is located the periphery side of pipe body.

Preferably, the inner surface of the annular snap-fit portion is in interference fit with the outer surface of the pipe body.

Preferably, the inner periphery of the annular clamping portion forms a first annular protrusion, and the outer periphery of the pipe body forms a second annular protrusion matched and clamped with the first annular protrusion.

Preferably, the first annular protrusion is located on one side of the annular clamping portion away from the shielding portion.

Preferably, the second annular projection extends from the open edge of the pipe body to the bottom of the pipe body.

Preferably, the cover further includes a pulling part attached to an outer periphery of the shielding part.

Preferably, the PCR tube further comprises a flexible connecting piece fixedly connected between the tube body and the cover body, one side of the flexible connecting piece encircles the periphery of the tube body, and the other side is fixedly connected with the cover body.

Preferably, the flexible connector is integrally formed with the cap body.

Preferably, the cover is made of polyethylene or polypropylene.

Preferably, the material of the pipe body is polyethylene or polypropylene.

Due to the implementation of the above technical scheme, compared with the prior art, the utility model have the following advantage:

the utility model discloses a PCR pipe, when the lid closed on the pipe body, the annular joint portion of lid and the periphery face joint of pipe body lived, thus, the lid is when covering, only the internal surface of occlusion part has the possibility of contacting this internal liquid of pipe, the annular joint portion of lid all contactless sample, can not cause the pollution, and simultaneously, in PCR reaction process, the hot lid of PCR appearance is pressed in PCR pipe top, the lid is compressed tightly very easily, with the pipe body on along in close contact with, can not leak gas, improve the gas tightness, improve the detectivity, avoid adopting to drip liquid paraffin and cover and the paraffin interference that causes detects the problem, improve operation personnel's operating efficiency.

Drawings

FIG. 1 is a schematic cross-sectional view of the PCR tube of the present invention (with the cover closed);

FIG. 2 is a schematic cross-sectional view of the PCR tube of the present invention (with the cover open);

wherein: 11. a shielding portion; 12. an annular clamping portion; 120. a first annular projection; 13. a pulling part; 20. a tube body;

201. a second annular projection; 30. a flexible connector.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.

As shown in fig. 1 and 2, the PCR tube includes a tube body 20 and a cover body for covering an opening of the tube body 20, the cover body includes a shielding portion 11 for shielding the opening of the tube body 20, and an annular engaging portion 12 connected to the shielding portion 11, and when the cover body is covered on the tube body 20, the annular engaging portion 12 is located on an outer peripheral side of the tube body 20. The inner surface of the shielding part 11 is a plane, and when the cover body covers the tube body 20, the inner surface of the shielding part 11 is attached to the opening edge of the tube body 20, so that the sealing property is better; in this embodiment, the cover is made of polyethylene or polypropylene, the pipe body 20 is made of polyethylene or polypropylene, and the cover is made of a flexible material and can be plastically deformed when the cover is closed or opened.

Specifically, the inner surface of the annular snap-fit portion 12 is interference-fitted with the outer surface of the pipe body 20. Therefore, the annular clamping part 12 can be attached to the pipe body 20 more tightly, so that the air tightness of the cover body after covering is ensured, and the detection sensitivity is improved.

In this example, the inner periphery of the annular engaging portion 12 is formed with a first annular protrusion 120, and the outer periphery of the tube main body 20 is formed with a second annular protrusion 201 engaged with the first annular protrusion 120. The first annular protrusion 120 is located on a side of the annular clamping portion 12 away from the shielding portion 11. The second annular protrusion 201 extends from the opening edge of the pipe body 20 toward the bottom of the pipe body 20. The first annular protrusion 120 and the second annular protrusion 201 are clamped and connected in a matching manner, so that the cover body can not be accidentally opened due to the stress on the edge of the cover body when the cover body is covered on the pipe body 20, and the experiment is ensured to be stably carried out.

Further, the cover body includes a pulling portion 13 connected to the outer periphery of the shielding portion 11. The pulling part 13 facilitates the worker to lift the entire cover from the pipe body 20 after pinching the pulling part 13, thereby improving the convenience of operation.

Further, the PCR tube further comprises a flexible connecting member 30 fixedly connected between the tube body 20 and the cover body, one side of the flexible connecting member 30 surrounds the periphery of the tube body 20, and the other side is fixedly connected with the cover body. The flexible connector and the cover body are integrally formed. A lid docks with a pipe body 20 through a flexible connectors 30, and the convenience of taking when the lid closes can avoid the lid to take off from pipe body 20 in addition and then put other places and cause the pollution, also avoids the indiscriminate scene chaotic condition of putting the cause of lid.

Compared with the traditional PCR tube, the utility model adopts the cover body full-plane design, thereby avoiding the pollution caused by the contact between the cylindrical convex complex structure on the cover of the traditional PCR tube and the sample; in the PCR reaction process, a hot cover of the PCR instrument is pressed above the PCR tube, and the cover body is easily pressed, so that the PCR instrument has better sealing property; the design of the traction piece around the cover body leads the operation to be more time-saving and simple; meanwhile, the simple design of the invention greatly reduces the production and manufacturing cost. In addition, the invention has great significance for clinical gene detection, can greatly reduce the false positive rate of clinical detection, and reduce unnecessary pain and economic loss of patients.

Use no ribonuclease water as the template, use the utility model discloses PCR pipe 10 is managed 10 with traditional PCR and is carried out real-time fluorescence ration PCR simultaneously and compare the experiment, and the result discovery, traditional PCR pipe have 3 false positives appearing, and the gas leakage phenomenon appears, use the utility model discloses a PCR pipe does not have gas leakage, does not have false positive.

To sum up, the utility model discloses a PCR pipe, when the lid closed on the pipe body, the annular joint portion of lid and the periphery face joint of pipe body lived, thus, the lid is when covering, only the internal surface of occlusion part has the possibility of contacting this internal liquid of pipe, the annular joint portion of lid all contactless sample, can not cause the pollution, and simultaneously, in PCR reaction process, the hot lid of PCR appearance is pressed in PCR pipe top, the lid is compressed tightly very easily, with the pipe body on along in close contact with, can not leak gas, improve the gas tightness, improve detectivity, avoid adopting liquid paraffin to cover and the paraffin interference detection's that causes problem, improve operation personnel's operating efficiency.

The present invention has been described in detail, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and to implement the same, and the protection scope of the present invention should not be limited thereby, and all equivalent changes or modifications made according to the spirit of the present invention should be covered by the protection scope of the present invention.

Claims (10)

1. The utility model provides a PCR pipe, PCR pipe includes the pipe body and is used for closing the lid of pipe body opening, its characterized in that: the cover body comprises a shielding part for shielding the opening of the pipe body and an annular clamping part connected to the shielding part, and when the cover body covers the pipe body, the annular clamping part is positioned on the outer peripheral side of the pipe body.

2. The PCR tube of claim 1, wherein: the inner surface of the annular clamping portion is in interference fit with the outer surface of the pipe body.

3. The PCR tube of claim 1, wherein: the inner periphery of the annular clamping portion is formed with a first annular bulge, and the outer periphery of the pipe body is formed with a second annular bulge matched with the first annular bulge in clamping connection.

4. The PCR tube of claim 3, wherein: the first annular bulge is positioned on one side, far away from the shielding part, of the annular clamping part.

5. The PCR tube of claim 3, wherein: the second annular protrusion extends from the opening edge of the pipe body to the bottom of the pipe body.

6. The PCR tube of claim 1, wherein: the cover body further comprises a drawing part connected to the periphery of the shielding part.

7. The PCR tube of claim 1, wherein: the PCR tube also comprises a flexible connecting piece fixedly connected between the tube body and the cover body, one side of the flexible connecting piece encircles the periphery of the tube body, and the other side of the flexible connecting piece is fixedly connected with the cover body.

8. The PCR tube of claim 7, wherein: the flexible connecting piece and the cover body are integrally formed.

9. The PCR tube of claim 1, wherein: the cover body is made of polyethylene or polypropylene.

10. The PCR tube of claim 1, wherein: the pipe body is made of polyethylene or polypropylene.

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