CN211235688U - Purifying column device for gel purifying instrument - Google Patents
Purifying column device for gel purifying instrument Download PDFInfo
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- CN211235688U CN211235688U CN201921867667.6U CN201921867667U CN211235688U CN 211235688 U CN211235688 U CN 211235688U CN 201921867667 U CN201921867667 U CN 201921867667U CN 211235688 U CN211235688 U CN 211235688U
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Abstract
A purifying column device for a gel purifying instrument belongs to the field of analytical instruments. A fixed sealing plug, a pipe joint and a liquid pipeline are arranged at two ends of a glass purification column; arranging a protective cover on the periphery of the glass purification column, and fixing the glass purification column, the fixed sealing plug and the adjusting sealing plug in the protective cover; the adjusting sealing plug is screwed in the upper gland and is fixed at the upper end of the glass purifying column through a locking nut; the protective cover, the upper gland, the lower gland and the glass purifying column positioned in the protective cover are fixed on the gel purifying instrument by the fixing plate; an observation window is arranged on the protective cover; the height of the gel purification filler in the glass purification column is adjusted by rotating the adjusting sealing plug. Can be widely applied to the design or manufacture field of gel purifiers with various specifications.
Description
Technical Field
The utility model belongs to the analytical instrument field especially relates to a purify post device for gel purifies appearance.
Background
At present, when pesticide residues in traditional Chinese medicine samples, environmental samples and food samples are analyzed, extracts of general samples contain a large amount of macromolecular substances, such as pigments, grease, sugar and the like, and the macromolecules are gathered on a sample inlet and an analysis column of a gas chromatograph, so that the chromatographic separation efficiency is reduced. Therefore, such samples must be effectively decontaminated prior to analysis.
Gel Permeation Chromatography (GPC) has proven to be one of the most convenient sample purification techniques to use as a means of sample purification.
The small molecular compounds can pass through the holes of the filler, and the large molecules pass through the space around the filler, so that the stroke difference between the small molecules and the large molecules can be caused, and the large molecules and the small molecules can flow out of the purifying column in sequence to play a role in separation. Since this separation technique is not affected by the polarity of the sample, it is a very effective means of purification for the chromatographic user.
When the sample flows through the gel purification column, if bubbles or faults exist among gel purification column fillers, small molecules may not pass through the gel purification fillers around the bubbles or the faults, and the small molecules run faster than the rest molecules and are discharged as waste liquid, so that the recovery rate is reduced. If this portion of the bubble passes through the gel column into the detector, the amount of uv absorption will be affected.
The bubbles have great influence on the gel purification result, so the tightness of the purification column device is ensured, and bubbles and faults generated by the bubbles are avoided.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that a purify post device for gel purifies appearance is provided. The sample is good in sealing performance, pressure-resistant, safe, convenient to observe, attractive in appearance and convenient to install, so that the micromolecular compound can penetrate through holes of the filler under the drive of the mobile phase, and macromolecules can penetrate through spaces around the filler, so that the good separation of large molecules and small molecules is realized, and the purification purpose is achieved. And a good guarantee is provided for the subsequent sample to enter an analysis instrument.
The technical scheme of the utility model is that: the purifying column device for the gel purifying instrument comprises a glass purifying column and sealing plugs positioned at two ends of the glass purifying column, wherein gel purifying fillers are arranged in the glass purifying column; the method is characterized in that:
a fixed sealing plug is arranged at the lower end of the glass purifying column; a lower liquid pipeline is arranged on the fixed sealing plug; a lower pipe joint is arranged at the joint of the fixed sealing plug and the lower liquid pipeline;
an upper liquid pipeline and an adjusting sealing plug are sequentially arranged at the upper end of the glass purification column at the joint of the adjusting sealing plug and the upper liquid pipeline, and an upper pipe joint is arranged;
an external thread structure is arranged on the periphery of the adjusting sealing plug, and a locking nut is sleeved on the periphery of the adjusting sealing plug; a knob is arranged at the upper end of the adjusting sealing plug;
a protective cover is arranged on the periphery of the glass purifying column; an upper gland is arranged at the upper end of the protective cover; a lower gland is arranged at the lower end of the protective cover;
screw-nut structures are respectively and correspondingly arranged between the protective cover and the upper gland, between the protective cover and the lower gland and between the upper gland and the adjusting sealing plug; the upper and lower pressure covers fix the glass purifying column, the fixed sealing plug and the adjusting sealing plug in the protective cover;
the adjusting sealing plug is screwed in the upper gland and is fixed at the upper end of the glass purifying column through a locking nut.
Wherein, the periphery of the fixed sealing plug or the adjusting sealing plug is provided with at least one sealing ring.
Specifically, two sealing rings are arranged on the periphery of the fixed sealing plug or the adjustable sealing plug.
Specifically, the knob is fixedly connected with the upper end of the adjusting sealing plug into a whole, and the rotating angle of the adjusting sealing plug is the same as that of the knob.
Further, a fixing plate with a mounting hole is arranged on the periphery of the protective cover
Specifically, the fixed plate fixes the protective cover, the upper gland, the lower gland and the glass purifying column in the protective cover on the gel purifying instrument.
Furthermore, an observation window is arranged on the protective cover.
The height of the gel purification filler in the glass purification column is adjusted by rotating the adjusting sealing plug.
Compared with the prior art, the utility model has the advantages that:
1. the adjusting sealing plug, the fixed sealing plug and the glass purifying column are connected through the elastic sealing ring, the sealing performance is good, the requirement on the tolerance of the inner diameter of the glass purifying column is low, and the glass purifying column is easy to process. At present, the glass processing level is limited to a certain degree, and the glass processing level cannot achieve higher precision like machining parts. The adjusting sealing plug, the fixed sealing plug and the glass purifying column are directly matched, certain risks exist in pressure resistance and sealing performance, and particularly, the liquid leakage risk exists when the pressure in the column is higher after a user uses the glass purifying column for a period of time. The sealing structure in the technical scheme has good sealing effect because the sealing ring has certain elasticity, and the tolerance of the inner diameter of the glass purification column can be enlarged, so that the processing difficulty and the rejection rate of the glass purification column are reduced.
2. The glass purification column is hooped in the middle by the protective cover with higher strength and the upper and lower pressing covers, so that the purification column can bear higher pressure without leakage, and the glass purification column can not cause injury to users even if the glass purification column is broken. By the higher glass purification post safety cover of intensity among this technical scheme, contain the safety cover, go up gland and gland down, its material can be metal material or the higher non-metallic material of intensity. The glass purifying column and the fixed sealing plug are hooped in the middle by the structure, the end face sealing is enhanced, and the pressure resistance of the purifying column device is improved. In addition, even if the glass purifying column is broken, the user can not be hurt due to the protection of the protective cover.
3. When the gel purification filler is filled, the height of the gel purification filler can be ensured by rotating and adjusting the sealing plug. The length of purifying column and the packing height all have strict requirements, and through threaded connection between adjusting sealing plug and the last gland in the purifying column device of this technical scheme, guarantee the accurate height of gel purification packing through the fine setting of rotatory regulation sealing plug.
4. The adjusting sealing plug is locked through double nuts, so that the purifying column can bear higher pressure, the adjusting sealing plug cannot move upwards, and the purifying column device is stable and reliable. The adjusting sealing plug is in threaded connection with the upper gland, and a micro screw withdrawing phenomenon can occur under the condition of large pressure after long-term use. Therefore, the purifying column device can bear larger pressure, and the adjusting sealing plug cannot move upwards.
5. The purification column device is provided with the fixing plate device, so that the purification column can be conveniently arranged on the gel purification instrument, and the device is attractive in appearance, safe and reliable. Because the purification post device is all that the columniform is fixed more difficult to it is external to be convenient for observe, the laboratory is all fixed with the mode of iron stand platform and general purpose clamp combination at present. The appearance is not pleasing to the eye enough, is touched easily, influences experiment process and stability. According to the technical scheme, the purifying column device can be used for directly fixing the purifying column on the gel purifying instrument, so that the purifying column and the gel purifying instrument are integrated, the appearance is attractive, stable and reliable, and the experiment can be ensured to be smoothly and stably carried out.
Drawings
FIG. 1 is a schematic sectional view of the purification column device of the present invention;
FIG. 2a is a partial external view of the protective cover of the present invention;
fig. 2b is a schematic cross-sectional view taken along line L-L of fig. 2 a.
In the figure, 1-1 is an upper liquid pipeline, 1-2 is a lower liquid pipeline, 2-1 is an upper pipe joint, 2-2 is a lower pipe joint, 3 is a knob, 4 is an adjusting sealing plug, 5 is a locking nut, 6 is an upper gland, 7 is a protective cover, 7-1 is a fixing plate mounting position, 7-2 is an observation window, 8 is a glass purification column, 9 is a fixing plate, 9-1 is a mounting hole, 10 is gel purification packing, 11 is a sealing ring, 12 is a fixing sealing plug, and 13 is a lower gland.
Detailed Description
The present invention will be further explained with reference to the drawings and examples.
The technical scheme of this application provides a leakproofness well, withstand voltage, safety, be convenient for observe, the appearance is pleasing to the eye, simple to operate's clean column device for the sample can pass from the hole of packing under the drive of mobile phase micromolecular compound, and the macromolecule passes from the space around the packing, realizes the fine separation of big small molecule, plays the purification purpose. And a good guarantee is provided for the subsequent sample to enter an analysis instrument.
As shown in fig. 1, the purifying column device of the present technical solution includes a glass purifying column 8 and sealing plugs at two ends thereof, wherein a gel purifying filler 10 is disposed in the glass purifying column 8; the invention is characterized in that:
a fixed sealing plug 12 is arranged at the lower end of the glass purification column; a lower liquid pipeline 1-2 is arranged on the fixed sealing plug 12; a lower pipe joint 2-2 is arranged at the joint of the fixed sealing plug and the lower liquid pipeline; an upper liquid pipeline 1-1 and an adjusting sealing plug 4 are sequentially arranged at the upper end of the glass purifying column; and an upper pipe joint 2-1 is arranged at the joint of the adjusting sealing plug and the upper liquid pipeline.
An external thread structure is arranged on the periphery of the adjusting sealing plug, and a locking nut 5 is sleeved on the periphery of the adjusting sealing plug; at the upper end of the adjusting sealing plug, a knob 3 is arranged.
A protective cover 7 is arranged on the periphery of the glass purification column; an upper gland 6 is arranged at the upper end of the protective cover; a lower gland 13 is arranged at the lower end of the protective cover; screw-nut structures are respectively and correspondingly arranged between the protective cover and the upper gland, between the protective cover and the lower gland and between the upper gland and the adjusting sealing plug; the upper and lower press covers fix the glass purifying column, the fixed sealing plug and the adjusting sealing plug in the protective cover.
The adjusting sealing plug is screwed in the upper gland and is fixed at the upper end of the glass purifying column through a locking nut.
As shown in fig. 1, at least one sealing ring (two sealing rings are taken as an example in the figure) is arranged on the periphery of the fixed sealing plug or the adjustable sealing plug.
Obviously, the knob is fixedly connected with the upper end of the adjusting sealing plug into a whole, and the rotating angle of the adjusting sealing plug is the same as that of the knob.
Further, a fixing plate 9 having a mounting hole 9-1 is provided at the outer periphery of the protective cover. The fixing plate fixes the protective cover, the upper and lower pressing covers, and the glass purifying column in the protective cover on the gel purifying instrument (not shown).
The working principle of the technical scheme is as follows:
the sample flows into a closed glass purifying column 8 from a liquid pipeline 1-2 at the lower end under the drive of a mobile phase through an external infusion pump, gel purifying fillers 10 are uniformly filled in the glass purifying column 8, small molecular compounds can pass through holes of the gel purifying fillers 10 under certain pressure, and macromolecules pass through spaces around the gel purifying fillers 10. Purified micromolecules and macromolecules flow out of the purification column 8 in sequence, enter the liquid pipeline 1-2 at the upper end, and collect required samples after purification, and waste discharge is carried out on the unnecessary samples.
In order to realize the functions, the whole assembly process of the purification column in the technical scheme is as follows:
the fixed sealing plug 12 provided with the two sealing rings 11, the lower liquid pipeline 1-2 and the lower pipe joint 2-2 is plugged at the lower end of the glass purification column 8, and a certain volume of the gel purification filler 10 is poured into the glass purification column 8. The upper gland 6, the locking nut 5 and the knob 3 are sequentially screwed into the adjusting sealing plug 4 through threads, then the two sealing rings 11, the upper liquid pipeline 1-1 and the upper pipe joint 2-1 are arranged at corresponding positions of the adjusting sealing plug 4, and then the arranged adjusting sealing plug 4 is plugged at the upper end of the glass purifying column 8. And (4) roughly adjusting the position of the sealing plug according to the height requirement of the gel purification filler. The two fixing plates 9 are fixed to the fixing plate mounting positions 7-1 of the protective cover 7, and the lower pressing cover 13 is screwed to the protective cover 7. The roughly adjusted gel purification column is arranged in a protective cover 7, an upper gland 6 is screwed on the protective cover 7 through threads, and a glass purification column 8 is limited between the upper gland 6 and a lower gland 13. At this time, the gel purging plug height can be finely adjusted by rotating the adjusting sealing plug 4. After the adjustment is finished, the locking nut 5 is screwed, and the phenomenon that the sealing plug 4 is slightly adjusted to slowly move upwards when the pressure in the column is high in the using process is prevented through a double-nut locking mode. The assembled purifying column can be fixed on the gel purifying instrument through four mounting holes 9-1 on the fixing plate 9.
In fig. 2, the protective cover 7 is provided with an observation window 7-2. The color of the gel purification filler 10 in the glass purification column 8 can be clearly observed in real time through the observation window 7-2, and the experimental process of the sample flowing through the purification column is monitored.
In addition, a fixing plate mounting position 7-1 is also arranged on the body of the protective cover 7; the aforementioned fixing plate 9 is fastened by screws at the mounting position 7-1 of the protective cover 7.
According to the purification column device in the technical scheme, the adjusting sealing plug, the fixed sealing plug and the glass purification column are connected through the elastic sealing ring, so that the sealing performance is good, the requirement on the tolerance of the inner diameter of the glass purification column is low, and the processing is easy; the glass purifying column is hooped in the middle by the protective cover with higher strength and the upper and the lower pressing covers, so that the purifying column can bear higher pressure without leakage, and the glass purifying column can not cause injury to users even if broken; the accurate height of the gel purification column packing is ensured by rotating the adjusting sealing plug, the adjusting sealing plug is locked by double nuts, the purification column can bear higher pressure, the adjusting sealing plug cannot move upwards, and the purification column device is stable and reliable; the cylindrical purifying column device is provided with the fixing plate device, so that the purifying column can be conveniently arranged on the gel purifying instrument, and the gel purifying instrument has attractive appearance and reliable installation.
The utility model can be widely used in the design or manufacture field of gel purifiers with various specifications.
Claims (8)
1. A purifying column device for a gel purifying instrument comprises a glass purifying column and sealing plugs positioned at two ends of the glass purifying column, wherein gel purifying fillers are arranged in the glass purifying column; the method is characterized in that:
a fixed sealing plug is arranged at the lower end of the glass purifying column; a lower liquid pipeline is arranged on the fixed sealing plug; a lower pipe joint is arranged at the joint of the fixed sealing plug and the lower liquid pipeline;
an upper liquid pipeline and an adjusting sealing plug are sequentially arranged at the upper end of the glass purifying column; an upper pipe joint is arranged at the joint of the adjusting sealing plug and the upper liquid pipeline;
an external thread structure is arranged on the periphery of the adjusting sealing plug, and a locking nut is sleeved on the periphery of the adjusting sealing plug; a knob is arranged at the upper end of the adjusting sealing plug;
a protective cover is arranged on the periphery of the glass purifying column; an upper gland is arranged at the upper end of the protective cover; a lower gland is arranged at the lower end of the protective cover;
screw-nut structures are respectively and correspondingly arranged between the protective cover and the upper gland, between the protective cover and the lower gland and between the upper gland and the adjusting sealing plug; the upper and lower pressure covers fix the glass purifying column, the fixed sealing plug and the adjusting sealing plug in the protective cover;
the adjusting sealing plug is screwed in the upper gland and is fixed at the upper end of the glass purifying column through a locking nut.
2. The purifying column device for gel purifying instrument as claimed in claim 1, wherein at least one sealing ring is provided on the outer circumference of the fixing sealing plug or the adjusting sealing plug.
3. The purifying column device for gel purifying instrument as claimed in claim 2, wherein two sealing rings are provided on the outer circumference of the fixing sealing plug or the adjusting sealing plug.
4. The purifying column device for gel purifying instrument as claimed in claim 1, wherein the knob is fixed to the upper end of the regulating sealing plug, and the rotation angle of the regulating sealing plug is the same as that of the knob.
5. The purifying column device for gel purifying instrument as claimed in claim 1, wherein a fixing plate having a mounting hole is provided at an outer periphery of the protective cover.
6. The purifying column device for gel purifying instrument as claimed in claim 5, wherein the fixing plate fixes the protective cover, the upper and lower pressing covers, and the glass purifying column in the protective cover to the gel purifying instrument.
7. The purifying column device for gel purifying instrument as claimed in claim 1, wherein an observation window is provided on the protective cover.
8. The purifying column device for gel purifying instrument as claimed in claim 1, wherein the height of the gel purifying filler in the glass purifying column is adjusted by rotating the adjusting sealing plug.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921867667.6U CN211235688U (en) | 2019-10-31 | 2019-10-31 | Purifying column device for gel purifying instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921867667.6U CN211235688U (en) | 2019-10-31 | 2019-10-31 | Purifying column device for gel purifying instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211235688U true CN211235688U (en) | 2020-08-11 |
Family
ID=71915565
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921867667.6U Active CN211235688U (en) | 2019-10-31 | 2019-10-31 | Purifying column device for gel purifying instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211235688U (en) |
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2019
- 2019-10-31 CN CN201921867667.6U patent/CN211235688U/en active Active
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