CN205049535U - Preparation chromatogram device with parallel separation module formula - Google Patents
Preparation chromatogram device with parallel separation module formula Download PDFInfo
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- CN205049535U CN205049535U CN201520827784.5U CN201520827784U CN205049535U CN 205049535 U CN205049535 U CN 205049535U CN 201520827784 U CN201520827784 U CN 201520827784U CN 205049535 U CN205049535 U CN 205049535U
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Abstract
The utility model relates to a preparation chromatogram device with parallel separation module formula, the device include blender I, blender II, dynamic axial compression post, 7 parallelly connected entrapment equipment, 3 parallelly connected preparation chromatographic column, fraction collector together. The entrance point I of blender I connects in parallel and has chromatogram pump I, II, and I entrance point II with dynamic axial compression post of its exit end links to each other, and the exit end II of this developments axial compression post links the entrance points III that have detector I, the exit end III of detector I links entrance point IV, the cross valve I that has chromatogram pump III, entrapment equipment respectively through cross valve II, the exit end of entrapment equipment IV in proper order through cross valve I, III link to each other with the entrance point V of preparation chromatographic column, cross valve III connects in parallel through blender II has chromatogram pump IV, V, the exit end of preparation chromatographic column links to each other with fraction collector through cross valve IV, detector II in proper order. The utility model is simple in operation, practical, separation efficiency is high.
Description
Technical field
The utility model relates to a kind of device for the preparation of chromatogram, particularly relates to a kind of preparative chromatography device with parallel clastotype.
Background technology
After chromatography is just invented from eighties of last century, experienced by developing into of a century has become most important compartment analysis science today, is widely used in a lot of field.Chromatogram is mechanism with partition equilibrium, utilizes each component partition equilibrium difference in sample to reach the effect of separation.
Preparative chromatography technology is an emerging high efficient separation technology, has the advantages such as efficient, quick, stable, reproducible, on-line checkingi, has been widely used in the every field such as medicine, biological products, fine chemistry industry, environment.At present, several factors of being concerned about most preparative chromatography technology of user are as follows: the higher recovery, higher separation rate, larger single preparation amount, better technique reappearance.
Along with going deep into of Natural products research, preparative chromatography technology can not meet the requirement of experiment completely, can not meet the separation preparation of polycomponent, polyvoltine compound, complicated cut.
Utility model content
Technical problem to be solved in the utility model is to provide a kind of simple to operate, practical, preparative chromatography device with parallel clastotype that separation efficiency is high.
For solving the problem, a kind of preparative chromatography device with parallel clastotype described in the utility model, is characterized in that: this device comprises mixer I, mixer II, dynamic axial compression column, 7 catching apparatus be connected in parallel, 3 preparative chromatography post, fraction collectors be connected in parallel; The entrance point I of described mixer I is parallel with chromatogram pump I, chromatogram pump II, and its endpiece I is connected with the entrance point II of described dynamic axial compression column by pipeline, and the endpiece II of this dynamic axial compression column is connected with the entrance point III of detecting device I; The endpiece III of described detecting device I is connected with chromatogram pump III, the entrance point IV of described catching apparatus, four-way valve I respectively through four-way valve II; The endpiece IV of described catching apparatus is connected with the entrance point V of described preparative chromatography post through described four-way valve I, four-way valve III successively; Described four-way valve III is parallel with chromatogram pump IV, chromatogram pump V through described mixer II; The endpiece of described preparative chromatography post is connected with described fraction collector through four-way valve IV, detecting device II successively.
Described pipeline between described mixer I and described dynamic axial compression column is provided with sampling valve.
Described four-way valve II is connected with the entrance point IV of described catching apparatus through 8 passage bridge valves; Described 8 passage bridge valves are provided with waste liquid escape hole.
Described four-way valve I is connected with sewer pipe.
Each described catching apparatus adopts concentric circles to cut open light, and its length is 5cm ~ 20cm, and diameter is 1 ~ 3cm.
Filler in each described catching apparatus is any one in polystyrene, octadecylsilane chemically bonded silica, SephadexLH-20, methacrylic acid-divinyl base benzene polymer.
Chromatograph packing material in described preparative chromatography post is any one in anti-phase C18, anti-phase C8, ClickCD, ClickGlucose, polystyrene, methacrylic acid-divinyl base benzene polymer.
The utility model compared with prior art has the following advantages:
1, be provided with dynamic axial compression column in the utility model, the object of the natural products of complexity being carried out to primary separation can be reached.
2, catching apparatus is provided with in the utility model, enrichment can be carried out to eluate to concentrate, and then select suitable chromatographic column to be finally separated according to the character (molecular weight, polarity, nonpolar, electric charge) of eluate, have high, the dead adsorption rate of the recovery low, without the need to features such as heating, the requirement of the adsorption and desorption of natural products can be met.
3, the utility model has the multiple clastotypes such as reverse-phase chromatography-reverse-phase chromatography, reverse-phase chromatography-gel chromatography, reverse-phase chromatography-chromatography of ions, reverse-phase chromatography-polymer chromatography, 1 sample introduction can be realized, repeatedly parallel separation, finally reach ultimate separation object, meet the requirement of Separation of Natural Products complicacy completely.
4, the utility model is simple to operate, practical, has that separation efficiency is high, good separating effect, automaticity high, is applicable to the separation of middle Tibetan medicine.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, embodiment of the present utility model is described in further detail.
Fig. 1 is structural representation of the present utility model.
In figure: 1-chromatogram pump, I 2-chromatogram pump, II 3-mixer, I 4-sampling valve, 5-dynamic axial compression column, 6-detecting device, I 7-chromatogram pump, III 8-four-way valve, I 9-four-way valve II 10-8 passage bridge valve, 11-catching apparatus, 12-four-way valve, III 13-chromatogram pump, IV 14-chromatogram pump, V 15-preparative chromatography post, 16-four-way valve, IV 17-detecting device, II 18-fraction collector, 19-mixer II.
Embodiment
As shown in Figure 1, have a preparative chromatography device for parallel clastotype, this device comprises mixer I 3, mixer II (19), dynamic axial compression column 5,7 catching apparatus be connected in parallel 11,3 preparative chromatography post 15, fraction collectors 18 be connected in parallel.
The entrance point I of mixer I 3 is parallel with chromatogram pump I 1, chromatogram pump II 2, and its endpiece I is connected with the entrance point II of dynamic axial compression column 5 by pipeline, and the endpiece II of this dynamic axial compression column 5 is connected with the entrance point III of detecting device I 6; The endpiece III of detecting device I 6 is connected with chromatogram pump III 7, the entrance point IV of catching apparatus 11, four-way valve I 8 respectively through four-way valve II 9; The endpiece IV of catching apparatus 11 is connected with the entrance point V of preparative chromatography post 15 through four-way valve I 8, four-way valve III 12 successively; Four-way valve III 12 is parallel with chromatogram pump IV 13, chromatogram pump V 14 through mixer II 19; The endpiece of preparative chromatography post 15 is connected with fraction collector 18 through four-way valve IV 16, detecting device II 17 successively.
Wherein: the pipeline between mixer I 3 and dynamic axial compression column 5 is provided with sampling valve 4.
Four-way valve II 9 is connected with the entrance point IV of catching apparatus 11 through 8 passage bridge valves 10; 8 passage bridge valves 10 are provided with waste liquid escape hole.
Four-way valve I 8 is connected with sewer pipe.
Each catching apparatus 11 adopts concentric circles to cut open light, and its length is 5cm ~ 20cm, and diameter is 1 ~ 3cm.
Filler in each catching apparatus 11 is any one in polystyrene, octadecylsilane chemically bonded silica, SephadexLH-20, methacrylic acid-divinyl base benzene polymer.
Chromatograph packing material in preparative chromatography post 15 is any one in anti-phase C18, anti-phase C8, ClickCD, ClickGlucose, polystyrene, methacrylic acid-divinyl base benzene polymer.
Principle of work of the present utility model: natural products isolates different chromatographic components by preparative chromatography, these components have different character as: to wait electrically, polarity, nonpolar, molecular size range etc., there is component of different nature can carry out enrichment in different catching apparatus 11 simultaneously and concentrate, thus reach the concentrated object of different component Sync enrichment.Chromatogram pump is the component be adsorbed on catching apparatus 11 successively wash-out, then according to the character of component, select preparative chromatography post 15 to be again separated successively, such component can be separated completely on preparative chromatography post 15.
application example
(1) the preparation of sample: after Tibetan medicine grande passerage herb is crushed to 40 orders, obtain grande passerage herb powder, powder is 80% ethanol refluxing extraction 2 hours under temperature is the condition of 80 DEG C by the volumetric concentration of its quality 20 times, obtain sample solution after filtration, after solution decompression is concentrated, obtain grande passerage herb extract.
(2) the separation of sample: falcate crazyweed herb extract is injected the utility model and carries out Separation of Natural Products, is separated and obtains 7 different chromatographic fractions (L1-L7).
(3) the enrichment of cut concentrates: enter catching apparatus 11 by being separated the chromatographic fraction (L1-L7) obtained by 8 passage bridge valves 10, final enrichment method obtains 7 different components.
(4) the second-order separation preparation: chromatographic fraction in catching apparatus 11 (L1-L7) eluted successively, by the cut that elutes respectively by reverse chromatograms, reverse-phase chromatography, polymer chromatography, gel chromatography, gel chromatography, polymer chromatography, polymer chromatography.
(5) monomeric compound is collected: collect being separated by the second-order separation preparative chromatography the monomeric compound obtained.Obtain 12 compounds altogether, compound title is as follows: 7-melonia flavones, rhamnetin, anagyrine, 7-hydroxyl dihydro anthoxanthin, Huang Qinsu, 3 ', 4 ', 5 ', 7 '-tetrahydroxy flavanone, cyanidenon, glycyrrhizin, white poplar place, 2 ', 4 '-dihydroxy chalcone, Quercetin, 7-methoxy flavanone.
Claims (7)
1. there is a preparative chromatography device for parallel clastotype, it is characterized in that: this device comprises mixer I (3), mixer II (19), dynamic axial compression column (5), 7 catching apparatus be connected in parallel (11), 3 the preparative chromatography posts (15) be connected in parallel, fraction collectors (18); The entrance point I of described mixer I (3) is parallel with chromatogram pump I (1), chromatogram pump II (2), its endpiece I is connected by the entrance point II of pipeline with described dynamic axial compression column (5), and the endpiece II of this dynamic axial compression column (5) is connected with the entrance point III of detecting device I (6); The endpiece III of described detecting device I (6) is connected with chromatogram pump III (7), the entrance point IV of described catching apparatus (11), four-way valve I (8) respectively through four-way valve II (9); The endpiece IV of described catching apparatus (11) is connected with the entrance point V of described preparative chromatography post (15) through described four-way valve I (8), four-way valve III (12) successively; Described four-way valve III (12) is parallel with chromatogram pump IV (13), chromatogram pump V (14) through described mixer II (19); The endpiece of described preparative chromatography post (15) is connected with described fraction collector (18) through four-way valve IV (16), detecting device II (17) successively.
2. a kind of preparative chromatography device with parallel clastotype as claimed in claim 1, is characterized in that: the described pipeline between described mixer I (3) and described dynamic axial compression column (5) is provided with sampling valve (4).
3. a kind of preparative chromatography device with parallel clastotype as claimed in claim 1, is characterized in that: described four-way valve II (9) is connected with the entrance point IV of described catching apparatus (11) through 8 passage bridge valves (10); Described 8 passage bridge valves (10) are provided with waste liquid escape hole.
4. a kind of preparative chromatography device with parallel clastotype as claimed in claim 1, is characterized in that: described four-way valve I (8) is connected with sewer pipe.
5. a kind of preparative chromatography device with parallel clastotype as claimed in claim 1, is characterized in that: each described catching apparatus (11) adopts concentric circles to cut open light, and its length is 5cm ~ 20cm, and diameter is 1 ~ 3cm.
6. a kind of preparative chromatography device with parallel clastotype as claimed in claim 1, is characterized in that: the filler in each described catching apparatus (11) is any one in polystyrene, octadecylsilane chemically bonded silica, SephadexLH-20, methacrylic acid-divinyl base benzene polymer.
7. a kind of preparative chromatography device with parallel clastotype as claimed in claim 1, is characterized in that: the chromatograph packing material in described preparative chromatography post (15) is any one in anti-phase C18, anti-phase C8, ClickCD, ClickGlucose, polystyrene, methacrylic acid-divinyl base benzene polymer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520827784.5U CN205049535U (en) | 2015-10-26 | 2015-10-26 | Preparation chromatogram device with parallel separation module formula |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520827784.5U CN205049535U (en) | 2015-10-26 | 2015-10-26 | Preparation chromatogram device with parallel separation module formula |
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| CN205049535U true CN205049535U (en) | 2016-02-24 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105572266A (en) * | 2016-03-04 | 2016-05-11 | 聊城大学 | Preparation supercritical fluid chromatographic instrument with multi-way valve and separation and purification method of preparation supercritical fluid chromatographic instrument |
| CN105784859A (en) * | 2016-03-04 | 2016-07-20 | 聊城大学 | Separation and purification preparation chromatograph and method for preparation, separation and purification |
| CN107573396A (en) * | 2017-08-08 | 2018-01-12 | 湖北中医药大学 | A kind of method for preparing high-purity chenodeoxy cholic acid using chromatogram 3 and dynamic axial compression column chromatography purifying |
| CN109416349A (en) * | 2016-07-06 | 2019-03-01 | 株式会社岛津制作所 | Preparative liquid chromatography instrument |
| CN110018264A (en) * | 2019-05-13 | 2019-07-16 | 河南工业大学 | The method that multichannel integrates liquid chromatogram column device and carries out liquid phase preparation |
-
2015
- 2015-10-26 CN CN201520827784.5U patent/CN205049535U/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105572266A (en) * | 2016-03-04 | 2016-05-11 | 聊城大学 | Preparation supercritical fluid chromatographic instrument with multi-way valve and separation and purification method of preparation supercritical fluid chromatographic instrument |
| CN105784859A (en) * | 2016-03-04 | 2016-07-20 | 聊城大学 | Separation and purification preparation chromatograph and method for preparation, separation and purification |
| CN109416349A (en) * | 2016-07-06 | 2019-03-01 | 株式会社岛津制作所 | Preparative liquid chromatography instrument |
| US11047834B2 (en) | 2016-07-06 | 2021-06-29 | Shimadzu Corporation | Preparative liquid chromatograph |
| CN107573396A (en) * | 2017-08-08 | 2018-01-12 | 湖北中医药大学 | A kind of method for preparing high-purity chenodeoxy cholic acid using chromatogram 3 and dynamic axial compression column chromatography purifying |
| CN107573396B (en) * | 2017-08-08 | 2020-04-21 | 湖北中医药大学 | Method for purifying and preparing high-purity chenodeoxycholic acid by utilizing chromatography-3 and dynamic axial compression column chromatography |
| CN110018264A (en) * | 2019-05-13 | 2019-07-16 | 河南工业大学 | The method that multichannel integrates liquid chromatogram column device and carries out liquid phase preparation |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160224 Termination date: 20171026 |