CN211235513U

CN211235513U - Super-plane type optical fiber biological adsorption sensor

Info

Publication number: CN211235513U
Application number: CN201922291822.0U
Authority: CN
Inventors: 刘云岗; 王�琦; 宋行; 吴欧
Original assignee: Changzhou Jingyang Semiconductor Material Technology Co ltd
Current assignee: Changzhou Jingyang Semiconductor Material Technology Co ltd
Priority date: 2019-12-19
Filing date: 2019-12-19
Publication date: 2020-08-11
Anticipated expiration: 2029-12-19

Abstract

The utility model discloses a super plane optic fibre biosorption sensor, including being plane PMMA basement sensing unit, PMMA basement sensing unit's sensing district surface plating one deck metal film, non-sensing district surface coating one deck low refractive index photocuring glues, and this PMMA basement sensing unit's both ends utilize fiber coupler to connect multimode optical fiber respectively. The utility model provides a sensor compares in traditional sensor that directly plates metal film excitation surface plasmon resonance phenomenon on the optic fibre core, and it is big to have an area, and the plane degree is good, advantages such as easy operation.

Description

Super-plane type optical fiber biological adsorption sensor

Technical Field

The utility model relates to a biomass detects technical field, in particular to super plane optic fibre biological adsorption sensor.

Background

The basic principle of the wavelength modulation type optical fiber sensor based on the surface plasmon resonance effect is that light propagating in an optical fiber is used for exciting surface plasmons plated in a metal film of a sensitive area of the optical fiber to generate a resonance phenomenon, and when the external environment changes, the resonance wavelength of the resonance changes, so that the movement of the resonance wavelength can be observed through a spectrometer to detect the external changes, wherein the changes can be the changes of physical quantities such as temperature, salinity and the like, and can also be the changes of biomass such as biological adsorption or biomolecule concentration and the like. The optical fiber sensor has the advantages of small volume, short detection period, low detection cost, electromagnetic interference resistance, no generation of toxic and harmful substances in the detection process and the like, and has the advantages of attracting more and more attention in the field of biosensing, and the amount of samples required by single detection is less compared with that of a prism type surface plasmon resonance instrument.

The optical fiber biosensor based on the traditional cylindrical sensing area mainly has the following problems: 1. if the amount of the sample required by single detection is too small, the sample can be agglomerated due to the surface tension of the detected liquid, and even if the surface tension reduction treatment is carried out on the sensitive area of the sensor in advance, the detected liquid can not be uniformly distributed under the influence of gravity due to the special shape of the cylindrical surface; 2. if the method of directly immersing in the liquid to be detected is adopted to eliminate the influence of uneven distribution caused by the shape of the cylindrical sensing area in the detection process, great waste to the sample to be detected can be caused; 3. when a uniform field condition is applied to the detection condition, such as an electric field or a magnetic field in the same direction or illumination in the same direction, the sensor with the cylindrical sensing area is difficult to realize.

SUMMERY OF THE UTILITY MODEL

According to the measured object maldistribution that the optic fibre surface plasmon resonance sensor that is used for detecting the biomass exists at present that above-mentioned provided and can't apply unified field scheduling problem, the utility model provides a super plane optic fibre biosorption sensor. The sensor mainly utilizes a super-planar sensing unit taking PMMA as a substrate and multimode optical fibers of a plastic cladding quartz fiber core as sensing devices, can enable the gravity direction of the liquid to be measured to be the same, further enables the liquid to be measured to be uniformly distributed, and can be conveniently applied when a light field, an electric field or a magnetic field with the same size and direction needs to be applied. The utility model provides high to biological detection's accuracy and reduced because external condition is different and the error of introducing.

The utility model provides a technical scheme that its technical problem adopted is: a super-planar optical fiber biological adsorption sensor comprises a planar PMMA substrate sensing unit, wherein a layer of metal film is plated on the surface of a sensing area of the PMMA substrate sensing unit, a layer of low-refractive-index light curing glue is coated on the surface of a non-sensing area, and two ends of the PMMA substrate sensing unit are respectively connected with a multimode optical fiber through optical fiber couplers.

Furthermore, the sensing area of the PMMA substrate sensing unit is positioned in the central area of one side of the PMMA substrate sensing unit, and the rest area of the outer surface of the PMMA substrate sensing unit except the sensing area is a non-sensing area.

Further, the PMMA substrate of the PMMA substrate sensing unit is square or rectangular.

Further, the sensing area of the PMMA substrate sensing unit is square or rectangular or circular.

Furthermore, the PMMA substrate of the PMMA substrate sensing unit is 40-70 mm in length, 10-30 mm in width and 0.2-1.7 mm in thickness.

Furthermore, the sensing area of the PMMA substrate sensing unit is square, the length is 10-30 mm, and the width is 10-30 mm.

Furthermore, the metal film plated on the surface of the sensing area of the PMMA substrate sensing unit is a gold film with the thickness of 30-80 nm.

Preferably, the thickness of the gold film plated on the surface of the sensing area of the PMMA substrate sensing unit is 50 nm.

Further, the thickness of the low-refractive-index light curing glue coated on the surface of the non-sensing area of the PMMA substrate sensing unit is 0.05-0.3 mm.

Preferably, the thickness of the low-refractive-index light curing glue coated on the surface of the non-sensing area of the PMMA substrate sensing unit is 0.1 mm.

Furthermore, the refractive index of the low-refractive-index light-cured adhesive is 1.47-1.49.

Compared with the prior art, the utility model, following advantage and beneficial effect have:

1. the optical fiber sensor provided by the utility model can form the measured liquid with uniform thickness in the sensing area, and can not agglomerate due to the action of gravity;

2. compared with a D-type optical fiber sensor, the optical fiber sensor provided by the utility model has larger sensing area and is easier to operate;

3. the utility model eliminates the error caused by uneven distribution of the measured liquid without immersing the sensor into the measured liquid, thereby saving the measured sample;

4. the utility model discloses compare traditional sensing district and apply external field condition, like electric field, magnetic field or light field for the optical fiber sensor of cylinder type more easily.

Drawings

Fig. 1 is a schematic structural view of a super-planar optical fiber bio-absorption sensor based on the surface plasmon resonance effect according to the present invention;

fig. 2 is a schematic diagram of a sensor transmission spectrum proposed in the present invention.

Detailed Description

It should be noted that, in the present invention, the embodiments and features of the embodiments may be combined with each other without conflict. The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments in accordance with the invention. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.

Unless specifically stated otherwise, the relative arrangement of the components and steps, the numerical expressions, and numerical values set forth in these embodiments do not limit the scope of the present invention. Meanwhile, it should be understood that the sizes of the respective portions shown in the drawings are not drawn in an actual proportional relationship for the convenience of description. Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail but are intended to be part of the specification where appropriate. Any specific values in all examples shown and discussed herein are to be construed as exemplary only and not as limiting. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, further discussion thereof is not required in subsequent figures.

In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the orientation words such as "front, back, up, down, left, right", "horizontal, vertical, horizontal" and "top, bottom", etc. are usually based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and in the case of not making a contrary explanation, these orientation words do not indicate and imply that the device or element in question must have a specific orientation or be constructed and operated in a specific orientation, and therefore should not be construed as limiting the scope of the present invention: the terms "inner and outer" refer to the inner and outer relative to the profile of the respective component itself.

Spatially relative terms, such as "above … …," "above … …," "above … …," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial relationship to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if a device in the figures is turned over, devices described as "above" or "on" other devices or configurations would then be oriented "below" or "under" the other devices or configurations. Thus, the exemplary term "above … …" can include both an orientation of "above … …" and "below … …". The device may be otherwise variously oriented (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.

It should be noted that the terms "first", "second", and the like are used to define the components, and are only used for convenience of distinguishing the corresponding components, and if not stated otherwise, the terms have no special meaning, and therefore, the scope of the present invention should not be construed as being limited.

Examples

A super-plane type optical fiber biological adsorption sensor is shown in figure 1 and comprises a multi-mode optical fiber 1 and a plane type PMMA substrate sensing unit 2, wherein the multi-mode optical fiber comprises an incident light multi-mode optical fiber 11 and an emergent light multi-mode optical fiber 12, the incident light multi-mode optical fiber 11 and the emergent light multi-mode optical fiber 12 are respectively connected through an incident light coupling port 21 and an emergent light coupling port 22 of an optical fiber coupler and the PMMA substrate sensing unit 2, a layer of gold film 24 is plated on the surface of a sensing area 23 of the PMMA substrate sensing unit 2, a layer of low-refractive-index light curing glue is coated on the surface of a non-sensing area 25 of the PMMA substrate sensing unit 2, and the refractive index of the low-refractive-index light curing glue.

The preparation method of the super-planar optical fiber biological adsorption sensor based on the surface plasmon resonance effect comprises the following steps:

(1) intercepting two sections of multimode fibers 1 with the length of 50mm to be used as incident light multimode fibers 11 and emergent light multimode fibers 12, wherein the multimode fibers 1 are plastic cladding quartz fiber core multimode fibers with fiber core refractive indexes of 1.4679, cladding refractive indexes of 1.4613, fiber core diameters of 600 mu m and cladding diameters of 630 mu m and plastic coating layers removed, and grinding and polishing the end faces after intercepting;

(2) processing a PMMA material into a PMMA substrate sensing plane unit 2 in a plane shape, wherein the PMMA substrate sensing plane unit 2 is 54mm in length, 18mm in width and 0.6mm in thickness, and an incident light coupling port 21 and an emergent light coupling port 22 of the PMMA substrate sensing plane unit 2 are 0.6mm in width;

(3) the surface of a sensing area 23 of a PMMA substrate sensing unit 2 is plated with a layer of metal film 24, the width of the sensing area 23 of the PMMA substrate sensing unit 2 is 18mm, the length of the sensing area 23 is 18mm, the sensing area is located in the central area of the PMMA substrate sensing unit 2, the metal film 24 is a gold film with the thickness of 50nm, and various implementation schemes can be designed by utilizing the prior art, such as plating the sensing area 23 with the gold film through a vacuum ion beam sputtering instrument or plating the sensing area 23 with the gold film through a magnetron sputtering instrument.

(4) Coating a non-sensing area 25 of the PMMA substrate sensing unit 2 with low-refractive-index light curing glue, wherein the area of the non-sensing area 25 is the other surface of the outer surface of the PMMA substrate sensing unit 2 except the sensing area 23, and does not comprise the coupling port end faces of the incident light coupling port 21 and the emergent light coupling port 22, and the thickness of the light curing glue coated on the non-sensing area 25 is 0.1 mm.

In the embodiment, the manufacturing processes of the multimode optical fiber 1 and the PMMA substrate sensing unit 2 are relatively mature, the composite structure is constructed by combining the hyperplane and the surface plasmon resonance structure of the PMMA substrate on the basis of the prior art, the operation is more convenient, the sample amount used in single detection is less, the same external field is more suitable to be applied, and the popularization and application value is higher.

The sensor prepared in this embodiment adopts the following sensing method: after a detection sample is placed in the sensing area 23 of the PMMA substrate sensing unit 2, the detection sample forms a uniform water film on the sensing area 23 by applying the same light field, i.e. the same light intensity and light direction, and under the same light condition, the transmission spectrum generated by the sensor is as shown in fig. 2, wherein the ordinate 3 is transmittance and the abscissa 4 is wavelength, when the biomolecules adsorbed by the metal film 24 on the surface of the sensing area 23 become more, the metal film 24 is excited by light to generate a surface plasmon resonance effect, so that the transmission spectrum of the sensor generates a resonance trough 5, the offset 6 of the resonance trough 5 will not be affected by errors generated by uneven distribution and uneven external conditions, and the offset 6 of the resonance trough 5 will be more accurate.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention.

Claims

1. A super-plane type optical fiber biological adsorption sensor is characterized in that: the sensor comprises a planar PMMA substrate sensing unit, wherein a layer of metal film is plated on the surface of a sensing area of the PMMA substrate sensing unit, a layer of light curing glue with low refractive index is coated on the surface of a non-sensing area, and two ends of the PMMA substrate sensing unit are respectively connected with a multimode optical fiber by an optical fiber coupler.

2. The ultra-planar optical fiber bio-absorption sensor according to claim 1, wherein: the sensing area of the PMMA substrate sensing unit is positioned in the central area of one surface of the PMMA substrate sensing unit, and the rest area of the outer surface of the PMMA substrate sensing unit except the sensing area is a non-sensing area.

3. The ultra-planar optical fiber bio-absorption sensor according to claim 1, wherein: the PMMA substrate of the PMMA substrate sensing unit is square or rectangular; the sensing area of the PMMA substrate sensing unit is square, rectangular or circular.

4. The ultra-planar optical fiber bio-absorption sensor according to claim 3, wherein: the PMMA substrate of the PMMA substrate sensing unit is 40-70 mm in length, 10-30 mm in width and 0.2-1.7 mm in thickness.

5. The ultra-planar optical fiber bio-absorption sensor according to claim 3, wherein: the sensing area of the PMMA substrate sensing unit is square, the length is 10-30 mm, and the width is 10-30 mm.

6. The ultra-planar optical fiber bio-absorption sensor according to claim 1, wherein: the metal film plated on the surface of the PMMA substrate in the sensing area of the PMMA substrate sensing plane unit is a gold film with the thickness of 30-80 nm.

7. The ultra-planar optical fiber bio-absorption sensor according to claim 6, wherein: the thickness of a gold film plated on the surface of the sensing area of the PMMA substrate sensing unit is 50 nm.

8. The ultra-planar optical fiber bio-absorption sensor according to claim 1, wherein: the thickness of the low-refractive-index light curing glue coated on the surface of the non-sensing area of the PMMA substrate sensing plane unit is 0.05-0.3 mm.

9. The ultra-planar optical fiber bio-absorption sensor according to claim 1, wherein: the thickness of the low-refractive-index light curing glue coated on the surface of the non-sensing area of the PMMA substrate sensing unit is 0.1 mm.

10. The ultra-planar optical fiber bio-absorption sensor according to claim 1, wherein: the refractive index of the low-refractive-index light curing adhesive is 1.47-1.49.