CN211227146U

CN211227146U - Sampling device for RNA purification

Info

Publication number: CN211227146U
Application number: CN201922151261.4U
Authority: CN
Inventors: 李君�
Original assignee: Beijing Kangrun Chengye Biotechnology Co ltd
Current assignee: Beijing Kangrun Chengye Biotechnology Co ltd
Priority date: 2019-12-04
Filing date: 2019-12-04
Publication date: 2020-08-11
Anticipated expiration: 2029-12-04

Abstract

The utility model relates to the field of RNA purification devices, in particular to a sampling device for RNA purification, which comprises a base, a raw material test tube, a fixed plate and a sampling mechanism, wherein the base is also provided with a fixed groove, the bottom of the raw material test tube is placed in the fixed groove, the base is also provided with the fixed mechanism for fixing the raw material test tube, the base is also symmetrically provided with a lifting mechanism, the fixed plate is arranged above the base, the output end of the fixed mechanism is connected with the bottom of the fixed plate, the sampling mechanism comprises a sampling tube, a piston, a needle tube and a driving mechanism for driving the piston to slide along the inner wall of the sampling tube, the fixed plate is also provided with a clamping groove, the sampling tube is clamped in the clamping groove, the piston is movably connected in the sampling tube, the needle tube is fixedly connected with the bottom of the sampling tube, the fixed plate is also, the driving mechanism and the lifting mechanism are electrically connected with the controller, and the device improves the precision of the RNA sampling amount.

Description

Sampling device for RNA purification

Technical Field

The utility model relates to a RNA purification device field, concretely relates to sampling device is used in RNA purification.

Background

Ribonucleic acids are genetic information carriers in biological cells and in parts of viruses and viroids. RNA is a single strand formed by transcription by taking a strand of DNA as a template and using a base complementary pairing principle, has the main function of realizing the expression of genetic information on protein, and is a bridge in the process of transmitting the genetic information. the tRNA functions to carry amino acids that meet the requirements, and the mRNA is used as a template to synthesize protein.

When RNA is purified, the RNA needs to be sampled, but the current sampling amount is completely controlled manually, and the sampling amount precision is insufficient, so that a sampling device is needed, the sampling of the RNA can be controlled by a controller, and the sampling amount precision is improved.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a sampling device is used in RNA purification.

To achieve the purpose, the utility model adopts the following technical proposal:

the utility model provides a sampling device for RNA purification, including base, raw materials test tube, fixed plate and sampling mechanism, the base is rectangle platelike structure, still be provided with the fixed slot on the base, the bottom of raw materials test tube is placed in the fixed slot, still be provided with the fixed establishment that is used for the fixed materials test tube on the base, still the symmetry is provided with elevating system on the base, the fixed plate sets up the top at the base to fixed establishment's output all is connected with the bottom of fixed plate, sampling mechanism is including sampling tube, piston, needle tubing and drive piston along the gliding actuating mechanism of inner wall of sampling tube, still set up the draw-in groove on the fixed plate, the sampling tube card is established in the draw-in groove, piston swing joint is in the sampling tube, needle tubing fixed connection is in the bottom of sampling tube, actuating mechanism is including mount pad, a micro motor, The rack and the gear of setting at the rack lateral part, the bottom fixed connection of rack is in the upper end of piston, the mount pad sets up on the fixed plate, first micro motor fixed mounting is on the mount pad, gear and first micro motor's output fixed connection to gear and rack meshing are connected, still be provided with the controller on the fixed plate, actuating mechanism and elevating system all with controller electric connection.

As an optimized scheme of sampling device for RNA purification, elevating system sets up the fixing base on the base including two-way screw rod, guide post, direction slider, hinge bar and symmetry, two-way screw rod coupling is on the fixing base, the guide post sets up the lateral part at two-way screw rod, the symmetrical threaded connection of direction slider is on two-way screw rod, the bottom of hinge bar articulates on the direction slider that corresponds, the upper end swing joint of hinge bar has the fixed block, the fixed second micro motor that is provided with on the fixing base, the tip and the output fixed connection of second micro motor of two-way screw rod.

As a preferable mode of the sampling device for RNA purification, the fixing block is fixedly arranged at the bottom of the fixing plate by a fastening screw.

As a preferred scheme of the sampling device for RNA purification, the fixing mechanism comprises a threaded rod and an arc-shaped fixed pressing plate, the threaded rod is in threaded connection with the base, the end part of the threaded rod extends into the fixing groove, and one end of the threaded rod, which extends into the fixing groove, is in shaft connection with the fixed pressing plate.

As a preferable scheme of the sampling device for RNA purification, one end of the threaded rod, which is far away from the base, is provided with a rotating frame for driving the threaded rod to rotate.

As a preferable scheme of the sampling device for RNA purification, the rotating frame is sleeved with an anti-slip sleeve.

As a preferred embodiment of the sampling device for RNA purification, the bottom of the base is provided with a plurality of rubber legs.

The utility model has the advantages that:

1. this sampling device is used in RNA purification, the design has mount pad, first micro motor, rack, gear, sampling tube, piston and needle tubing, when needs sample, can open first micro motor drive gear and rotate, just can rise along vertical direction with gear engagement's rack, drives the piston and rises, just can realize taking a sample to the RNA in the raw materials test tube to by controller control, the volume that can the accurate control sample.

2. This sampling device for RNA purification, the design has threaded rod and curved fixed pressing plate, places the raw materials test tube back in the fixed slot, can drive the threaded rod and rotate, just can make the tip of threaded rod remove to the fixed slot, promotes fixed pressing plate and fixes the raw materials test tube in the fixed slot, prevents that the raw materials test tube from taking place to rock when the sample, leads to spilling etc. of raw materials.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required to be used in the embodiments of the present invention will be briefly described below. It is obvious that the drawings described below are only some embodiments of the invention, and that for a person skilled in the art, other drawings can be obtained from these drawings without inventive effort.

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is an enlarged schematic view at a in fig. 1.

Fig. 3 is a schematic view of the split structure of the present invention.

In the figure: the device comprises a base 1, a raw material test tube 2, a fixing plate 3, a sampling mechanism 4, a fixing groove 5, a fixing mechanism 6, a lifting mechanism 7, a sampling tube 8, a piston 9, a needle tube 10, a mounting seat 11, a first micro motor 12, a rack 13, a gear 14, a controller 15, a bidirectional screw 16, a guide column 17, a guide slider 18, a hinge rod 19, a fixing seat 20, a second micro motor 21, a threaded rod 22, a fixing pressing plate 23, a rotating frame 24 and a rubber leg frame 25.

Detailed Description

The technical solution of the present invention is further explained by the following embodiments with reference to the accompanying drawings.

Wherein the showings are for the purpose of illustration only and are shown by way of illustration only and not in actual form, and are not to be construed as limiting the present patent; for a better understanding of the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.

The same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar parts; in the description of the present invention, it should be understood that if the terms "upper", "lower", "left", "right", "inner", "outer", etc. are used to indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, it is only for convenience of description and simplification of description, but it is not indicated or implied that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and therefore, the terms describing the positional relationship in the drawings are used only for illustrative purposes and are not to be construed as limiting the present patent, and the specific meaning of the terms will be understood by those skilled in the art according to the specific circumstances.

In the description of the present invention, unless otherwise explicitly specified or limited, the term "connected" or the like, if appearing to indicate a connection relationship between the components, is to be understood broadly, for example, as being either a fixed connection, a detachable connection, or an integral part; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or may be connected through one or more other components or may be in an interactive relationship with one another. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1 to 3, the sampling device for RNA purification comprises a base 1, a raw material test tube 2, a fixing plate 3 and a sampling mechanism 4, wherein the base 1 is of a rectangular plate-shaped structure, the base 1 is further provided with a fixing groove 5, the bottom of the raw material test tube 2 is placed in the fixing groove 5, the base 1 is further provided with a fixing mechanism 6 for fixing the raw material test tube 2, the base 1 is further symmetrically provided with a lifting mechanism 7, the fixing plate 3 is arranged above the base 1, the output end of the fixing mechanism 6 is connected with the bottom of the fixing plate 3, the sampling mechanism 4 comprises a sampling tube 8, a piston 9, a needle tube 10 and a driving mechanism for driving the piston 9 to slide along the inner wall of the sampling tube 8, the fixing plate 3 is further provided with a clamping groove, the sampling tube 8 is clamped in the clamping groove, and the piston 9 is movably connected in the sampling tube 8, needle tubing 10 fixed connection is in the bottom of sampling tube 8, actuating mechanism is including mount pad 11, first micro motor 12, rack 13 and set up the gear 14 at the rack 13 lateral part, the bottom fixed connection of rack 13 is in the upper end of piston 9, mount pad 11 sets up on fixed plate 3, first micro motor 12 fixed mounting is on mount pad 11, gear 14 and first micro motor 12's output fixed connection to gear 14 is connected with the meshing of rack 13, still be provided with controller 15 on the fixed plate 3, actuating mechanism and elevating system 7 all with controller 15 electric connection. Controller 15's model is DDCVS 3.1, when the user equipment takes a sample, at first place raw materials test tube 2 in fixed slot 5, then drive fixed establishment 6 fixes raw materials test tube 2, then drive elevating system 7 descends, make the needle tubing 10 of 8 bottom installations of sampling tube can stretch into raw materials test tube 2 in, then controller 15 controls first micro motor 12 drive gear 14 and rotates, rack 13 with 14 meshing of gear just can rise along vertical direction, it rises to drive piston 9, just can realize carrying out the accurate sample to the RNA in the raw materials test tube 2, at last with the sample from the sampling tube 8 take out can.

Elevating system 7 sets up fixing base 20 on base 1 including two-way screw rod 16, guide post 17, direction slider 18, hinge bar 19 and symmetry, two-way screw rod 16 coupling is on fixing base 20, guide post 17 sets up the lateral part at two-way screw rod 16, the symmetrical threaded connection of direction slider 18 is on two-way screw rod 16, the bottom of hinge bar 19 articulates on the direction slider 18 that corresponds, the upper end swing joint of hinge bar 19 has the fixed block, the fixed second micro motor 21 that is provided with on fixing base 20, the tip of two-way screw rod 16 and second micro motor 21's output fixed connection.

The fixed block is fixedly arranged at the bottom of the fixed plate 3 through a fastening screw. When the dead plate 3 needs to be lowered, the dead plate 3 can be lowered by driving the bidirectional screw 16 to rotate so that the guide sliders 18 symmetrically screwed on the bidirectional screw 16 move outwards, and the needle tube 10 can be inserted into the raw material for sampling.

The fixing mechanism 6 comprises a threaded rod 22 and an arc-shaped fixing pressing plate 23, the threaded rod 22 is in threaded connection with the base 1, the end part of the threaded rod 22 extends into the fixing groove 5, and one end, extending into the fixing groove 5, of the threaded rod 22 is in shaft connection with the fixing pressing plate 23. Place raw materials test tube 2 behind fixed slot 5, rotate threaded rod 22 and just can make threaded rod 22's tip remove to fixed slot 5, promote stationary platen 23 to fix raw materials test tube 2 in fixed slot 5.

And a rotating frame 24 for driving the threaded rod 22 to rotate is arranged at one end of the threaded rod 22 far away from the base 1.

The rotating frame 24 is sleeved with an anti-slip sleeve.

The bottom of the base 1 is provided with a plurality of rubber legs 25.

The working principle is as follows: when using the device to take a sample, at first place raw materials test tube 2 in fixed slot 5, then drive fixed establishment 6 fixes raw materials test tube 2, then drive elevating system 7 descends, make the needle tubing 10 of 8 bottom installations of sampling tube can stretch into raw materials test tube 2 in, then the first micro motor 12 drive gear 14 of controller 15 control rotates, just can rise along vertical direction with rack 13 of gear 14 meshing, it rises to drive piston 9, just can realize carrying out the accurate sample to the RNA in the raw materials test tube 2, at last with the sample from the sampling tube 8 take out can.

It should be understood that the above-described embodiments are merely illustrative of the preferred embodiments of the present invention and the technical principles thereof. It will be understood by those skilled in the art that various modifications, equivalents, changes, and the like can be made to the present invention. However, these modifications are within the scope of the present invention as long as they do not depart from the spirit of the present invention. In addition, certain terms used in the specification and claims of the present application are not limiting, but are used merely for convenience of description.

Claims

1. A sampling device for RNA purification, characterized in that: comprises a base (1), a raw material test tube (2), a fixing plate (3) and a sampling mechanism (4), wherein the base (1) is of a rectangular plate-shaped structure, the base (1) is further provided with a fixing groove (5), the bottom of the raw material test tube (2) is placed in the fixing groove (5), the base (1) is further provided with a fixing mechanism (6) for fixing the raw material test tube (2), the base (1) is further symmetrically provided with a lifting mechanism (7), the fixing plate (3) is arranged above the base (1), the output end of the fixing mechanism (6) is connected with the bottom of the fixing plate (3), the sampling mechanism (4) comprises a sampling tube (8), a piston (9), a needle tube (10) and a driving mechanism for driving the piston (9) to slide along the inner wall of the sampling tube (8), and the fixing plate (3) is further provided with a clamping groove, the sampling tube (8) is clamped in the clamping groove, the piston (9) is movably connected in the sampling tube (8), the needle tube (10) is fixedly connected with the bottom of the sampling tube (8), the driving mechanism comprises a mounting seat (11), a first micro motor (12), a rack (13) and a gear (14) arranged on the side part of the rack (13), the bottom of the rack (13) is fixedly connected with the upper end of the piston (9), the mounting seat (11) is arranged on the fixed plate (3), the first micro motor (12) is fixedly arranged on the mounting seat (11), the gear (14) is fixedly connected with the output end of the first micro motor (12), the gear (14) is meshed with the rack (13), the fixed plate (3) is also provided with a controller (15), the driving mechanism and the lifting mechanism (7) are electrically connected with the controller (15).

2. The RNA purification sampling device of claim 1, wherein: elevating system (7) sets up fixing base (20) on base (1) including two-way screw rod (16), guide post (17), direction slider (18), hinge rod (19) and symmetry, two-way screw rod (16) coupling is on fixing base (20), guide post (17) set up the lateral part at two-way screw rod (16), direction slider (18) symmetry threaded connection is on two-way screw rod (16), the bottom of hinge rod (19) articulates on corresponding direction slider (18), the upper end swing joint of hinge rod (19) has the fixed block, fixed second micro motor (21) that is provided with on fixing base (20), the tip of two-way screw rod (16) and the output fixed connection of second micro motor (21).

3. The RNA purification sampling device of claim 2, wherein: the fixed block is fixedly arranged at the bottom of the fixed plate (3) through a fastening screw.

4. The RNA purification sampling device of claim 3, wherein: fixed establishment (6) are including threaded rod (22) and curved fixed pressing plate (23), threaded rod (22) threaded connection is on base (1) to the tip of threaded rod (22) extends to in fixed slot (5), one end and fixed pressing plate (23) coupling in threaded rod (22) extend to fixed slot (5).

5. The RNA purification sampling device of claim 4, wherein: and a rotating frame (24) for driving the threaded rod (22) to rotate is arranged at one end, far away from the base (1), of the threaded rod (22).

6. The RNA purification sampling device of claim 5, wherein: the rotating frame (24) is sleeved with an anti-slip sleeve.

7. The RNA purification sampling device of claim 6, wherein: the bottom of the base (1) is provided with a plurality of rubber leg frames (25).