CN211206533U - Multipurpose micro-fluid detection card - Google Patents
Multipurpose micro-fluid detection card Download PDFInfo
- Publication number
- CN211206533U CN211206533U CN201922010575.2U CN201922010575U CN211206533U CN 211206533 U CN211206533 U CN 211206533U CN 201922010575 U CN201922010575 U CN 201922010575U CN 211206533 U CN211206533 U CN 211206533U
- Authority
- CN
- China
- Prior art keywords
- detection
- structure layer
- detection card
- waste liquid
- bottom plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to a multipurpose micro-fluidic detection card, this micro-fluidic detection card include bottom plate, upper cover, microchannel structure layer and absorbent paper, and the microchannel structure layer is located between upper cover and the bottom plate layer to cover the application of sample hole that has the intercommunication with the upper cover, the microchannel structure layer includes detection zone and waste liquid district, and absorbent paper is located between microchannel structure layer and the bottom plate, and is located waste liquid district below, and the application of sample hole has interior hemofiltration membrane and mark thing fixed bolster. The utility model discloses an increase and strain blood membrane and marker fixed bolster, can realize the detection to whole blood, carry out the dilatation through the integrated detection card waste liquid district position that will absorb water paper to make the detection card can satisfy the detection of step by step detection and high content sample.
Description
Technical Field
The utility model relates to an analysis and detection field, concretely relates to multipurpose micro-fluid detection card.
Background
The microfluidic technology is a technology for controlling, operating and detecting complex fluid under a microscopic size, is a brand new interdisciplinary developed on the basis of microelectronics, micromachines, bioengineering and nanotechnology, and has the advantages that the consumption of a sample and a reagent thereof is greatly reduced and the detection speed is effectively improved through the microfluidic operation in a microchannel; the microfluid technology also has the characteristics of small volume, portability and the like, thereby being beneficial to developing a bedside real-time monitoring technology, using the microfluid detection technology to detect multiple targets can further reduce the detection cost, and the detection accuracy and specificity of the multiple targets can be improved by the same detection.
Patent CN201610023958.1 provides a detect self-driven micro-fluidic detection card of many targets, this detection card includes the bottom plate, the upper cover and the double-sided adhesive layer that is located between upper cover and the bottom plate, wherein double-sided adhesive layer has the microchannel, the used sample volume of this detection card is little, the detectivity is high, detection range is wide, but this patent only can realize the detection to serum, can not realize the detection to whole blood, and this detection card waste liquid district volume is little, to the detection of some markers, if carry out the flow of washing, the background interference of sample itself is difficult to eliminate, thereby the application scope of detection card has been restricted. For example, when hemoglobin and glycated hemoglobin are detected by using a fluorescence method using a detection card, if a washing process is not performed, a fluorescent signal is absorbed greatly when the sample itself is dark, and thus it is difficult to obtain a signal value of a detection area. And for the detection of the marker with larger content in the sample, if the one-step reaction is adopted for the detection of the marker, the Hooke effect is often generated, and the result is that the test generates false positive or false negative; the Hooke effect can be completely avoided by adopting a step-by-step method for detection. For example, when the CRP is detected, the concentration of the CRP in the sample cannot be accurately judged by adopting the one-step detection method, but the waste liquid generated by the step-by-step detection is large, and the microfluidic detection card described in patent CN201610023958.1 cannot meet the requirements, so the detection card must be improved to completely meet the use requirements of the washing liquid in the step-by-step detection washing step, thereby avoiding the occurrence of false positive or false negative results, and expanding the application range of the detection card.
Therefore, the establishment of a microfluid detection technology which is simple and convenient to manufacture, does not depend on external force to drive, can realize the detection of whole blood, can realize the washing of a sample and the step-by-step detection of a marker with higher content has important significance.
Disclosure of Invention
The detection to whole blood can not be realized to the micro-fluid detection card that exists among the prior art, and detect that card waste liquid district volume is little, can not realize detecting the technical problem who washes with the marker substep, the utility model provides a multipurpose micro-fluid detection card has not only realized the detection to the higher marker of content in the sample, can realize the detection to whole blood moreover.
The utility model discloses a following technical scheme realizes:
a multipurpose microfluidic detection card comprises a bottom plate, an upper cover, a microchannel structure layer and absorbent paper;
the microchannel structure layer is positioned between the upper cover and the bottom plate;
the microchannel structure layer and the upper cover are provided with sample adding holes which are communicated;
the microchannel structure layer comprises a detection area and a waste liquid area;
the absorbent paper is positioned between the micro-channel structure layer and the bottom plate and is positioned below the waste liquid area.
The absorbent paper absorbs the waste liquid in the waste liquid area, and the capacity expansion is carried out in a mode that the absorbent paper is integrated to the position of the waste liquid area of the detection card, so that the detection card can meet the requirement of step-by-step reaction, the waste liquid area of the original detection card can contain the liquid with the volume of only 100 mu L, and after the capacity expansion, the liquid containing volume can reach more than 300 mu L.
Preferably, the sample adding hole is internally provided with a blood filtering membrane and a marker fixing pad.
Preferably, the marker fixing pad is positioned below the blood filtering membrane, and the blood filtering membrane can filter blood cells in the whole blood and only pass through serum by adding the blood filtering membrane and the marker fixing pad, so that the detection of the whole blood is realized.
Preferably, the upper cover further comprises a vent hole.
Preferably, the bottom plate, the upper cover and the microchannel structure layer are assembled and fixed through the positioning holes.
Preferably, the upper cover is made of transparent material.
Advantageous effects
(1) The utility model discloses an increase and strain blood membrane and marker fixed bolster, can realize the detection to whole blood.
(2) The utility model provides a detection card has carried out the dilatation through the mode of paper that will absorb water integrated to detection card waste liquid district position to make the detection card can satisfy the demand of substep reaction, former detection card waste liquid district can hold the liquid volume and only 100 mu L, after the dilatation, can hold the liquid volume and can reach more than 300 mu L.
Drawings
FIG. 1 is an exploded view of the microfluidic test card of the present invention;
FIG. 2 is an overall view of the microfluidic test card of the present invention;
the kit comprises a blood filtering membrane 1, a marker fixing pad 3, an upper cover 4, a micro-channel structure layer 5, absorbent paper 6, a bottom plate 7, air holes 8, sample adding holes 9, a detection area 10, a waste liquid area 11 and a positioning hole, wherein the blood filtering membrane 2 is arranged on the bottom plate, and the marker fixing pad 3 is arranged on the bottom plate;
FIG. 3 is a graph showing the effect of CRP concentration of the microfluidic assay card on the value of the assay signal in example 1;
FIG. 4 is a graph showing the effect of the microfluidic detection card of comparative example 1 on the detection signal value.
Detailed Description
For better explanation of the present invention, the present invention will be described in detail with reference to the accompanying drawings and specific embodiments, which are only illustrative and not intended to limit the scope of the present invention.
Fig. 1 is an exploded view of the microfluidic test card of the present invention, and fig. 2 is an overall view of the microfluidic test card of the present invention;
wherein, this miniflow body detects the card and includes bottom plate 6, upper cover 3, microchannel structure layer 4 and absorbent paper 5, microchannel structure layer 4 is located between upper cover 3 and bottom plate 6, there is the application of sample hole 8 of intercommunication on microchannel structure layer 4 and the upper cover 3, microchannel structure layer 4 includes detection zone 9 and waste liquid district 10, 5 levels of absorbent paper are located between microchannel structure layer 4 and bottom plate 6, and be located the below of waste liquid district 10, absorbent paper 5 absorbs the waste liquid of waste liquid district 10, there are blood filtering membrane 1 and marker fixed bolster 2 in the application of sample hole 8, marker fixed bolster 2 is located blood filtering membrane 1 below, bottom plate 6, upper cover 3, microchannel structure layer 4 is fixed through the equipment of locating hole 11, upper cover 1 includes that bleeder vent 7 upper cover 3 is transparent material.
Example 1
Comparative example 1
Comparative example 2 the detection of CRP by using the microfluidic detection card in patent CN201610023958.1 only enables the one-step detection of CRP, and the effect of CRP concentration on the detection signal value is shown in fig. 4.
It is thus clear that adopt the utility model provides a micro-fluidic detection card can realize detecting the marker that content is high in the sample, can realize detecting the washing and the substep of sample moreover, and the signal value is high.
The foregoing is only a preferred embodiment of this patent, and it should be noted that, for those skilled in the art, various modifications and substitutions can be made without departing from the technical principle of this patent, and these modifications and substitutions should also be regarded as the protection scope of this patent.
Claims (7)
1. A multipurpose microfluidic detection card is characterized by comprising a bottom plate (6), an upper cover (3), a microchannel structure layer (4) and absorbent paper (5);
the microchannel structure layer (4) is positioned between the upper cover (3) and the bottom plate (6);
the microchannel structure layer (4) and the upper cover (3) are provided with sample adding holes (8) which are communicated;
the microchannel structure layer (4) comprises a detection area (9) and a waste liquid area (10);
the absorbent paper (5) is positioned between the micro-channel structure layer (4) and the bottom plate (6) and is positioned below the waste liquid area (10).
2. Microfluidic detection card according to claim 1, characterized in that the absorbent paper (5) absorbs waste liquid from the waste liquid zone (10).
3. Microfluidic detection card according to claim 1, characterized in that the loading well (8) contains a hemofilter (1) and a label holding pad (2).
4. Microfluidic detection card according to claim 3, characterized in that the label holder pad (2) is located below the hemofilter membrane (1).
5. The microfluidic detection card of claim 1, wherein the bottom plate (6), the top cover (3), and the microchannel structure layer (4) are assembled and fixed through the positioning holes (11).
6. Microfluidic test card according to claim 1, characterized in that the cover (3) further comprises a vent (7).
7. Microfluidic detection card according to claim 1, characterized in that the cover (3) is a transparent material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922010575.2U CN211206533U (en) | 2019-11-19 | 2019-11-19 | Multipurpose micro-fluid detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922010575.2U CN211206533U (en) | 2019-11-19 | 2019-11-19 | Multipurpose micro-fluid detection card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211206533U true CN211206533U (en) | 2020-08-07 |
Family
ID=71851704
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922010575.2U Active CN211206533U (en) | 2019-11-19 | 2019-11-19 | Multipurpose micro-fluid detection card |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211206533U (en) |
-
2019
- 2019-11-19 CN CN201922010575.2U patent/CN211206533U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11123731B2 (en) | Microfluidic detection chip, preparation method thereof, fixing device and centrifugal detection device | |
| CN103537329B (en) | The control method and test device of microfluidic device and the microfluidic device | |
| CN202886374U (en) | Device for carrying out multiple tests | |
| EP1963840B1 (en) | Immunoassay apparatus and kit | |
| CN103827324B (en) | Device and method for detecting an analyte | |
| US20150290639A1 (en) | Pressure assisted lateral flow diagnostic device | |
| CN101571538B (en) | Patterned biochemical analysis test paper and manufacturing method and application thereof | |
| WO2007002580A3 (en) | Diagnostic as say system with multi -well reagent container | |
| JP2012513773A5 (en) | ||
| WO2003012443A3 (en) | Rapid diagnostic device, assay and multifunctional buffer | |
| WO2006130299A2 (en) | Microfluidic laminar flow detection strip | |
| KR20150101307A (en) | Microfluidic device | |
| CN107991484B (en) | Rotary electrochemical immune paper chip analysis device and detection method thereof | |
| JP2004294388A (en) | Component-analyzing apparatus | |
| EP3985391A1 (en) | Magnetic particle light-emitting double-layer micro-fluidic chip and detection system | |
| CN215783449U (en) | Self-driven micro-fluid detection card capable of controlling flow rate | |
| WO2019131606A1 (en) | Inspection device | |
| JP2005265685A (en) | Plasma component analyzing device | |
| US20210308666A1 (en) | Assay plates, separation sheets, filters, and sample deposition marks | |
| CN211206533U (en) | Multipurpose micro-fluid detection card | |
| US11266988B2 (en) | Small volume self-metered blood separation device | |
| KR102520865B1 (en) | The pressure-type sampling device | |
| CN107748160B (en) | POCT ready-to-use micro-fluidic chip and application method | |
| CN210954058U (en) | Magnetic bead releasing mechanism and system | |
| KR20140147327A (en) | Fluid Analysis Cartridge |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |