CN211179857U

CN211179857U - Single-channel optical detection device for quantum dot fluorescence multi-detection

Info

Publication number: CN211179857U
Application number: CN201922113953.XU
Authority: CN
Inventors: 张乐之; 吴敏华
Original assignee: Hangzhou Kangzhi Biomedical Co ltd
Current assignee: Hangzhou Kangzhi Biomedical Co ltd
Priority date: 2019-11-29
Filing date: 2019-11-29
Publication date: 2020-08-04
Anticipated expiration: 2029-11-29

Abstract

The utility model relates to a quantum dot fluorescence multi-detection single-channel optical detection device which can not only simultaneously carry out the joint detection of a plurality of groups of projects on the same serum sample, but also reduce the blood-drawing amount of a patient, a placing frame is arranged in a detection bin, a reaction cup or a reaction plate is placed on the placing frame, and an excitation light source is arranged on the detection bin; one side of the detection bin is provided with a plurality of filter chambers, and the plurality of filter chambers are respectively provided with one optical filter, and the wavelengths of visible light which can be transmitted by the optical filters are different; the light splitter is arranged in the detection bin, when excitation light irradiates a substrate to be detected to generate fluorescence, the fluorescence can be emitted into an incident end of the light splitter, the light splitter is provided with a plurality of emitting ends, and the ends of the emitting ends are positioned in the corresponding filter chambers. The advantages are that: the utility model provides a single channel optical detection device is examined in many ways to quantum dot fluorescence not only can carry out the joint inspection of multiunit project to same portion serum sample simultaneously, can reduce patient's volume of drawing blood moreover, both can improve work efficiency, and detection effect is good simultaneously, is the new platform of liquid phase spot test (POCT).

Description

Single-channel optical detection device for quantum dot fluorescence multi-detection

Technical Field

The utility model relates to a fluorescence immunoassay technical field especially relates to a single channel optical detection device is examined in many associations of quantum dot fluorescence.

Background

Quantum Dots (QD) are extremely tiny inorganic liquid nanocrystals, have the particle size of about 2-10 nanometers, mainly comprise II-VI group or III-V group elements, have the radius smaller than or close to the Bohr radius of laser, and can receive exciting light to generate fluorescence. Compared to other immunolabeling materials: the quantum dots have high fluorescence intensity, good stability and strong bleaching resistance; the excitation spectrum is wide, the emission spectrum is narrow and is symmetrically distributed, so that the quantum dots can be used for multicolor marking of multiple components, and multi-component synchronous detection is realized; the biocompatibility is good, and particularly after various chemical modifications, the specific connection can be carried out, the cytotoxicity is low, the harm to organisms is small, and the kit is more suitable for biological living body marking and detection.

Quantum Dot Nanospheres (QDNBs) are labels formed by adsorbing or embedding Quantum dots on a high polymer support. Generally, the synthesis is performed by layer-by-layer self-assembly or embedding. Research shows that the fluorescent signals of the quantum dots can be further amplified by embedding the quantum dots in the organic polymer (the fluorescent intensity of the quantum dot nanospheres under the same molar number is 2863 times that of the quantum dots), so that the immunodetection sensitivity is remarkably improved. Meanwhile, the quantum dots are protected by the polymer, so that quenching of fluorescence can be avoided to a certain extent, the stability of the quantum dots can be effectively improved, and in addition, the polymer surface is modified by functional groups, so that the biological application range of the quantum dots is wider.

Shenzhen Jinquan biomedical engineering Limited initiates a quantum dot immunochromatography technology platform globally. Immunochromatography (ICTS) is a detection technique that combines membrane chromatography with immunolabeling. As a rapid diagnostic reagent, the reagent has the advantages of simple and convenient operation, short detection time, low cost, suitability for Point-of-care test (POCT) and the like, and is widely applied to the detection of biomedicine, food safety, pesticide residues and environmental pollutants. Labeling materials such as colloidal gold, colored latex, upconverting particles, etc. are often introduced into immunochromatography to visualize the antigen-antibody reaction at the detection region. The disadvantage of this technology platform is that it is not conducive to high quality joint inspection of samples.

SUMMERY OF THE UTILITY MODEL

The design purpose is as follows: in order to avoid the defects in the background art, the quantum dot fluorescence multi-detection single-channel optical detection device is designed, which not only can simultaneously carry out the joint detection of a plurality of groups of projects on the same serum sample, but also can reduce the blood volume of a patient.

The design scheme is as follows: the design purpose is realized.

1. An excitation light source is arranged on the detection bin, and light rays generated by the excitation light source can irradiate the substrate to be detected in the reaction cup or the reaction plate; a plurality of filter chambers are arranged on one side of the detection bin, and a filter is arranged in each filter chamber, and the wavelengths of visible light which can be transmitted by each filter are different; detect the storehouse and be equipped with the spectroscope, wait to detect the incident end that fluorescence can jet into the spectroscope when the substrate produces fluorescence exciting light irradiation, the spectroscope is equipped with the design that a plurality of ejection ends and a plurality of ejection end are located corresponding the filter chamber, is one of the technical characteristics of the utility model. The purpose of this design is: the quantum dot fluorescence multi-detection single-channel optical detection device can perform multi-group project joint detection on the same serum sample at the same time, so that the detection efficiency can be improved, and the blood extraction amount for patient detection can be reduced; and secondly, a plurality of filter chambers are arranged in the device, so that mutual interference among the transmitted lights can be avoided when the photoelectric conversion device or the camera is used for shooting and detecting.

2. The optical splitter comprises a plurality of optical fibers, an optical fiber bundling sleeve, a bundling sleeve support frame and a plurality of optical fiber support frames; one side end of many optic fibres forms the end of gathering a bundle and the end of gathering a bundle passes through a set of bundle cover support frame fixed mounting in detecting the storehouse through optic fibre set bundle cover, the opposite side end of many optic fibres is through the design of an optic fibre support frame fixed mounting in corresponding the filter chamber respectively, is the utility model discloses a two technical characteristics. The purpose of this design is: the reflected light is conducted by the optical fiber, and the fluorescence can be irradiated into each filter chamber, thereby improving the detection effect of the device.

3. Photoelectric conversion device or camera lens just to a plurality of filter chambers and to the design that a plurality of filter chambers are located photoelectric conversion device or camera lens's effective receipt fluorescence within range, it is the technical characteristics of the utility model three. The purpose of this design is: the photoelectric conversion device or the camera lens is over against the plurality of filter chambers and the plurality of filter chambers are positioned in the effective fluorescence receiving range of the photoelectric conversion device or the camera lens, so that the quantum dot fluorescence multi-inspection single-channel optical detection device can simultaneously realize the joint inspection of a plurality of groups of items.

4. The design of a side face of the placing frame and the inner side face fixed connection of the bin gate is the fourth technical characteristic of the utility model. The purpose of this design is: one side surface of the placing frame is fixedly connected with the inner side surface of the bin gate, so that the reaction cup or the reaction plate can be conveniently placed.

5. When the reaction cup or the reaction plate is made of opaque material, the incident ends of the excitation light source and the light splitter are both located right above the reaction cup or the reaction plate, which is the fifth technical feature of the present invention. The purpose of this design is: the incidence ends of the excitation light source and the spectroscope in the detection unit are both positioned above the reaction cup, so that the device can detect the substrate in the reaction cup or the reaction plate when the reaction cup or the reaction plate is a non-transparent cup.

6. The excitation light source and the incident end of the light splitter are respectively arranged on the two sides of the reaction cup or the reaction plate, which is the sixth technical characteristic of the utility model. The purpose of this design is: when the reaction cup is made of a light-transmitting material, the incident ends of the excitation light source and the light splitter in the detection unit are positioned at two sides of the reaction cup or the reaction plate, so that the device can flexibly set the positions of the incident ends of the excitation light source and the light splitter at two sides of the reaction cup or the reaction plate, and the structural layout of the device is more reasonable.

The technical scheme is as follows: a quantum dot fluorescence multi-detection single-channel optical detection device comprises a detection bin, wherein a placing frame is arranged in the detection bin, a reaction cup or a reaction plate is placed on the placing frame, an excitation light source is arranged on the detection bin, and light rays generated by the excitation light source can irradiate a substrate to be detected in the reaction cup or the reaction plate; a plurality of filter chambers are arranged on one side of the detection bin, and a filter is arranged in each filter chamber, and the wavelengths of visible light which can be transmitted by each filter are different; the light filter is characterized in that a light splitter is arranged in the detection bin, when excitation light irradiates a substrate to be detected to generate fluorescence, the fluorescence can be emitted into an incident end of the light splitter, the light splitter is provided with a plurality of emitting ends, and the ends of the emitting ends are positioned in the corresponding light filter chambers.

Compared with the prior art, the utility model, a single channel optical detection device is examined more to quantum dot fluorescence not only can carry out the joint inspection of multiunit project simultaneously to same part serum sample, can reduce patient's volume of drawing blood moreover, and detection effect is good simultaneously.

Drawings

Fig. 1 is a structural schematic diagram of a quantum dot fluorescence multi-detection single-channel optical detection device (an excitation light source and a beam splitter are located on two sides of a detection chamber).

Fig. 2 is a schematic diagram of the structure of the optical splitter.

Fig. 3 is a structural schematic diagram of a quantum dot fluorescence multi-detection single-channel optical detection device (an excitation light source and a light splitter are located at the top of a detection chamber).

Detailed Description

Example 1: reference is made to figures 1-3 of the drawings. A quantum dot fluorescence multi-detection single-channel optical detection device comprises a detection bin 1, wherein a placing frame 2 is arranged in the detection bin 1, a reaction cup or a reaction plate is placed on the placing frame 2, an excitation light source 3 is arranged on the detection bin 1, and light rays generated by the excitation light source 3 can irradiate a substrate to be detected in the reaction cup or the reaction plate; a plurality of filter chambers 4 are arranged on one side of the detection bin 1, and a filter is respectively arranged in each filter chamber 4, and the wavelengths of visible light which can be transmitted by each filter are different; the detection chamber 1 is internally provided with a light splitter 5, when excitation light irradiates a substrate to be detected to generate fluorescence, the fluorescence can be emitted into an incident end of the light splitter 5, the light splitter 5 is provided with a plurality of emitting ends, and the ends of the emitting ends are positioned in the corresponding filter chambers 4. The end heads of a plurality of ejection ends of the light splitter 5 are opposite to the corresponding light filters; and a hole for the photoelectric conversion device to detect or the camera to shoot is formed in the outer side of the filter chamber 4. The wavelength of the excitation light source 3 is about 365 nm.

The optical splitter 5 comprises a plurality of optical fibers 51, an optical fiber bundling sleeve 52, a bundling sleeve support frame and a plurality of optical fiber support frames; one end of each of the optical fibers 51 forms a bundling end through the optical fiber bundling sleeve 52, the bundling end is fixedly mounted in the detection bin 1 through a bundling sleeve support frame, and the other end of each of the optical fibers 51 is fixedly mounted in the corresponding filter chamber 4 through one optical fiber support frame.

The fluorescent lamp further comprises a photoelectric conversion device or a camera lens 6, wherein the photoelectric conversion device or the camera lens 6 is over against the plurality of filter chambers 4, and the plurality of filter chambers 4 are located in the effective fluorescence receiving range of the photoelectric conversion device or the camera lens 6. One side of the detection bin 1 is provided with a bin door 7. One side surface of the placing frame 2 is fixedly connected with the inner side surface of the bin gate 7. When the reaction cup or the reaction plate is made of opaque material, the incident ends of the excitation light source 3 and the light splitter 5 are both located right above the reaction cup or the reaction plate. When the reaction cup or the reaction plate is made of a light-transmitting material, the incident ends of the excitation light source 3 and the light splitter 5 are respectively positioned at two sides of the reaction cup or the reaction plate. The wavelength of the visible light transmitted by each filter is within a specific range, and the wavelengths of the visible light transmitted by the plurality of filters cover the whole wavelength range of the visible light.

When the kit is used, a plurality of different detection reagents are added into the same reaction cup or reaction plate, and a corresponding substrate to be detected can be simultaneously formed in the reaction cup, for example, three different detection reagents (detection reagent A, detection reagent B, detection reagent C) are added into a serum sample of the same reaction cup or reaction plate, and finally the corresponding substrate to be detected A, substrate to be detected B, and substrate to be detected C are obtained in the reaction cup or reaction plate, under the irradiation of an excitation light source 3, the wavelength of fluorescence (emission light) generated by the substrate to be detected A is 400nm-500nm, the wavelength of fluorescence (emission light) generated by the substrate to be detected B is 500nm-600nm, and the wavelength of fluorescence (emission light) generated by the substrate to be detected C is 600nm-700 nm; when the reaction cup or the reaction plate is put into the detection bin 1, the excitation light source 3 generates excitation light to irradiate substrates to be detected (the substrates to be detected comprise a substrate A to be detected, a substrate B to be detected and a substrate C to be detected) in the reaction cup or the reaction plate, then the substrates to be detected simultaneously emit emission light with the wavelength of 400nm-500nm, emission light with the wavelength of 500nm-600nm and emission light with the wavelength of 600nm-700nm, the mixed emission light is divided into three beams by the light splitter 5 to respectively irradiate the light filters in the three light filter chambers 4, because the light filter in the first light filter chamber can only pass visible light with the wavelength of 400nm-500nm, the light filter in the second light filter chamber can only pass visible light with the wavelength of 500nm-600nm, and the light filter in the third light filter chamber can only pass visible light with the wavelength of 600nm-700nm, at the moment, the quantum dot fluorescence multi-detection single-channel optical detection device can simultaneously perform joint detection on the detection items corresponding to the substrate A, the substrate B and the substrate C, namely, the quantum dot fluorescence multi-detection single-channel optical detection device can simultaneously perform joint detection on multiple groups of items on the same serum sample.

It is to be understood that: although above-mentioned embodiment is right the utility model discloses a design has done more detailed word description, but these word descriptions, are only right the utility model relates to a simple word description of idea, rather than right the utility model relates to a restriction of idea, any do not surpass the utility model relates to a combination, increase or the modification of idea all fall into the utility model discloses a within the protection scope.

Claims

1. The utility model provides a single channel optical detection device is examined in many associations to quantum dot fluorescence, is including detecting storehouse (1), be equipped with in detecting storehouse (1) and placed reaction cup or reaction plate on dispenser (2), characterized by: an excitation light source (3) is arranged on the detection bin (1), and light rays generated by the excitation light source (3) can irradiate the substrate to be detected in the reaction cup or the reaction plate; a plurality of filter chambers (4) are arranged on one side of the detection bin (1), and a filter is arranged in each filter chamber (4), and the wavelengths of visible light which can be transmitted by each filter are different; the fluorescence detection device is characterized in that a light splitter (5) is arranged in the detection bin (1), when excitation light irradiates a substrate to be detected to generate fluorescence, the fluorescence can be emitted into an incident end of the light splitter (5), the light splitter (5) is provided with a plurality of emitting ends, and the ends of the emitting ends are located in the corresponding filter chambers (4).

2. The quantum dot fluorescence multi-detection single-channel optical detection device of claim 1, which is characterized in that: the optical splitter (5) comprises a plurality of optical fibers (51), an optical fiber bundling sleeve (52), a bundling sleeve support frame and a plurality of optical fiber support frames; one side ends of the optical fibers (51) form a bundling end through an optical fiber bundling sleeve (52), the bundling end is fixedly installed in the detection bin (1) through a bundling sleeve support frame, and the other side ends of the optical fibers (51) are fixedly installed in the corresponding filter chambers (4) through an optical fiber support frame respectively.

3. The quantum dot fluorescence multi-detection single-channel optical detection device of claim 1, which is characterized in that: the fluorescent lamp also comprises a photoelectric conversion device or a camera lens (6), wherein the photoelectric conversion device or the camera lens (6) is over against the plurality of filter chambers (4) and the plurality of filter chambers (4) are positioned in the effective fluorescence receiving range of the photoelectric conversion device or the camera lens (6).

4. The quantum dot fluorescence multi-detection single-channel optical detection device of claim 1, which is characterized in that: one side of the detection bin (1) is provided with a bin door (7).

5. The quantum dot fluorescence multi-inspection single-channel optical detection device of claim 4, wherein: one side surface of the placing frame (2) is fixedly connected with the inner side surface of the bin door (7).

6. The quantum dot fluorescence multi-detection single-channel optical detection device of claim 1, which is characterized in that: when the reaction cup or the reaction plate is made of opaque materials, the incident ends of the excitation light source (3) and the light splitter (5) are both positioned right above the reaction cup or the reaction plate.

7. The quantum dot fluorescence multi-detection single-channel optical detection device of claim 1, which is characterized in that: when the reaction cup or the reaction plate is made of a light-transmitting material, the incident ends of the excitation light source (3) and the light splitter (5) are respectively positioned at two sides of the reaction cup or the reaction plate.