CN211149053U - Turntable switching type multiband L ED fluorescent device - Google Patents
Turntable switching type multiband L ED fluorescent device Download PDFInfo
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- CN211149053U CN211149053U CN201922320479.8U CN201922320479U CN211149053U CN 211149053 U CN211149053 U CN 211149053U CN 201922320479 U CN201922320479 U CN 201922320479U CN 211149053 U CN211149053 U CN 211149053U
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Abstract
The utility model relates to a carousel switching formula multiband L ED fluorescence device includes the base, the base is equipped with the carousel, carousel circumference is equipped with two at least L ED light sources and two at least fluorescence filter group, L ED light source and fluorescence filter group install the position at offside 180 degrees, the center of carousel is equipped with the illumination condensing lens, the base corresponds L ED light source and is equipped with brush mechanism, every fluorescence filter group is including exciting the piece, dichroscope and ending the piece, the below of dichroscope is equipped with the cut-off piece that the level set up, dichroscope and cut-off piece are certain angle, light is in proper order through exciting the piece, dichroscope and ending the piece, still include the device of restriction carousel rotation angle, L ED fluorescence excitation lighting device that accessible carousel mode switched, on the basis that does not change original fluorescence microscope structure, realize switching L ED light source and fluorescence filter group simultaneously through a carousel conversion, realize the excitation illumination of fast switch different wavelengths, combine L ED self long service life, advantages such as convenient operation and safe in utilization.
Description
Technical Field
The utility model relates to a fluorescence microscopic observation technique, concretely relates to carousel switching formula multiband L ED fluorescence device.
Background
The existing fluorescence microscope adopts three types of excitation light sources, namely a high-power ultrahigh-pressure mercury lamp, a xenon lamp and an L ED lamp.
The power of the high-power ultrahigh-pressure mercury lamp is generally 100W to 200W. When the lamp is electrified, the two electrodes discharge to cause mercury evaporation, the process takes 5 to 15 minutes, the pressure in the lamp bulb can reach 50 to 70 standard atmospheric pressures, and mercury molecules are continuously decomposed and reduced to emit light quanta during the two-electrode discharge. The method has the advantages that the spectrum has a plurality of brightness peaks, the peaks are distributed in a plurality of commonly used fluorescent wave bands such as ultraviolet, purple, blue and green, various fluorescent substances can be almost excited, and the applicability is very strong. However, the mercury lamp lighting needs a complex mercury lamp power supply box as accessory equipment, active heat dissipation is needed, the use noise is high, the operation is inconvenient and unsafe, and the mercury lamp cannot be switched on and off in real time; the bulb has the defects of short service life, easy explosion, large heat productivity, unadjustable brightness, higher use cost, great damage to eyes of operators and the like, and is a non-green lighting technology. Therefore, the existing high-power ultra-high pressure mercury lamp is not an ideal lighting device of a fluorescent microscope, and new technology is urgently needed to be improved.
The ultrahigh-pressure xenon lamp is manufactured by utilizing the discharge luminescence principle of inert gas under ultrahigh pressure. Its luminous spectrum is similar to solar spectrum, and is a continuous spectrum, its wave peak is 400 nm-450 nm, and its spectrum is smooth, and it has rich continuous ultraviolet rays, and is a good ultraviolet and blue light excitation light source. It has the advantages of convenient operation, immediate ignition during starting, no damage to the lamp bulb even if the lamp bulb is immediately extinguished after ignition, and long service life. The luminous lamp has the defects of stable arc light and unchanged color temperature in the luminous process, needs a special low-voltage direct-current power supply box, has extremely high temperature and is more expensive than a mercury lamp, so the luminous lamp is not widely used on a fluorescence microscope.
L ED is applied to a fluorescence microscope for many years, and a push-pull switching mode is adopted in the early stage, and the two modes are divided into a fluorescence filter set and a L ED light source switching mode and an independent switching mode.
SUMMERY OF THE UTILITY MODEL
There are a great deal of disadvantage to fluorescence microscope's use mercury lamp xenon lamp carousel mode switching and use L ED push-and-pull mode switching etc, the utility model provides a carousel switching formula multiband L ED fluorescence device, its compact structure, L ED fluorescence that accessible carousel mode switched arouses lighting device, on the basis that does not change original fluorescence microscope structure, realize switching L ED light source and fluorescence filter group simultaneously through a carousel conversion, realize the excitation illumination of different wavelength of fast switch, combine L ED self long service life, advantages such as convenient operation and safe in utilization, replace traditional mercury lamp or xenon lamp excitation illumination completely.
In order to solve the technical problem, the utility model discloses a realize through following technical scheme:
a turntable switching type multiband L ED fluorescent device comprises a base, wherein the base is provided with a turntable, the turntable is circumferentially provided with at least two L ED light sources and at least two fluorescent filter sets, the L ED light sources and the fluorescent filter sets are arranged at 180-degree opposite positions, a lighting condenser lens is arranged at the center of the turntable, the base is provided with an electric brush mechanism corresponding to the L ED light sources, each fluorescent filter set comprises an excitation sheet, a dichroic mirror and a stop sheet, a horizontally arranged stop sheet is arranged below the dichroic mirror, the dichroic mirror and the stop sheet form a certain angle, light rays sequentially pass through the excitation sheet, the dichroic mirror and the stop sheet, and the device further comprises a device for limiting the rotation angle of the turntable.
Further, the base is equipped with the mounting hole, be equipped with the location top pearl at spring and spring top in the mounting hole, every L ED light source and fluorescence filter group form a complete fluorescence illumination excitation piece group, and every group operating position corresponds location top pearl is equipped with positioning groove and corresponds location top pearl is equipped with positioning groove.
Further, the electric brush mechanism is provided with an electric brush thimble corresponding to the L ED light source.
Furthermore, the device for limiting the rotation angle of the rotary table comprises a limit screw and an arc-shaped accommodating groove, the limit screw is arranged at the bottom of the rotary table, the base corresponds to the limit screw and is provided with the arc-shaped accommodating groove, and the arc-shaped accommodating groove limits the movement track of the limit screw.
Furthermore, the rotary table further comprises an outer shell, an opening is formed in the side portion of the outer shell corresponding to the rotary table, and the edge of the rotary table extends out of the opening.
Furthermore, the shell is provided with a through hole corresponding to a fluorescence light path through which the dichroic mirror passes.
Further, the fluorescent power supply controller is connected with the electric brush mechanism.
Compared with the prior art, the beneficial effects of the utility model are that:
a carousel switching formula multiband L ED fluorescence device, the on-line screen storage device comprises a base, the base is equipped with the carousel, carousel circumference is equipped with two at least L ED light sources and two at least fluorescence filter group, L ED light source and fluorescence filter group install the position at offside 180 degrees, the center of carousel is equipped with the illumination condensing lens, the base corresponds L ED light source and is equipped with brush mechanism, every fluorescence filter group still is equipped with the dichroscope, the below of dichroscope is equipped with the cut-off piece that the level set up, dichroscope and cut-off piece are certain angle, still include the device of restriction carousel rotation angle, L ED fluorescence excitation lighting device that accessible carousel mode switched, on the basis that does not change original fluorescence microscope structure, realize switching L ED light source and fluorescence filter group simultaneously through a carousel conversion, realize the excitation illumination of the different wavelength of fast switch, combine L ED self long service life, advantages such as convenient operation and safe in utilization, replace traditional mercury lamp or xenon lamp excitation illumination completely.
The utility model discloses use changeable multiband L ED fluorescence excitation illumination to replace the mercury lamp to excite the illumination, can conveniently load in current fluorescence microscopic imaging system, further extend the product function, but wide application in biology, histology, cytology, microbiology, immunology and medical a great deal of field.
Drawings
The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, together with the embodiments of the invention, and do not constitute a limitation on the invention, in which:
FIG. 1 is an assembly view of the present invention;
FIG. 2 is a schematic view of the internal structure of the case after opening;
FIG. 3 is a schematic cross-sectional structure and light path diagram of the turntable structure.
In the figure, 1-base; 2-rotating disc;
3-screw, 4-L ED light source;
5-fluorescent filter set; 6-illumination condenser;
7-electric brush mechanism; 8-brush thimble;
9-exciting sheet; 10-dichroic mirror;
11-a cut-off tab; 12-mounting hole;
13-spring; 14-positioning the top bead;
15-positioning groove; 16-a limit screw;
17-a housing; 18-an opening;
19-through hole; 20-fluorescent power supply controller;
21-connection port; 22-power switch;
23-dimming knob; 24-objective lens.
Detailed Description
The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are presented herein only to illustrate and explain the present invention, and not to limit the present invention.
As shown in fig. 1 to 3, a carousel switching formula multiband L ED fluorescence device includes base 1, base 1 is equipped with carousel 2, carousel 2 is rotatable, carousel 2 passes through four L ED light sources 4 of screw 3 circumference installation and four fluorescence filter group 5, L ED light source 4 and fluorescence filter group 5 are installed in the position of offside 180 degrees, when observing different samples or the sample of different dyestuff dyeings, L ED light source 4 and the fluorescence filter group 5 that need change corresponding wave band just can switch different wave bands through rotatory carousel 2.
The center of the turntable 2 is provided with a lighting condenser lens 6, the base 1 is provided with an electric brush mechanism 7 corresponding to an L ED light source 4, the electric brush mechanism 7 is provided with two electric brush thimbles 8 corresponding to a L ED light source 4 and used for communicating the anode and the cathode of a circuit board at the back of the fluorescence power controller 20 and the L ED light source 4, L ED light is used for emitting light, when the L ED light source 4 is in a working position, the light source is turned on, when the L ED light source 4 is turned out, a L ED lamp is turned off, each fluorescence filter set 5 comprises an excitation sheet 9, a dichroic mirror 10 and a cut-off sheet 11, a horizontally arranged cut-off sheet 11 is arranged below the dichroic mirror 10, the dichroic mirror 10 and the cut-off sheet 11 are at an angle of 45 degrees, light sequentially passes through the excitation sheet 9, the dichroic mirror 10 and the cut-off sheet 11, and the fluorescence filter set 5 mainly aims to filter illumination light emitted by the light source into monochromatic light with.
Compared with the traditional illumination technology, the L ED light source 4 has the advantages of high efficiency, energy conservation, long service life, small volume, easy maintenance, environmental protection, safe use and the like, and is acknowledged to be the first choice of future illumination, in addition, L ED has good monochromaticity, the half width of a spectral line can be controlled to be 10nm magnitude, and the L ED is very suitable for fluorescent illumination, L ED with corresponding wave bands can be used from ultraviolet to infrared, however, L ED has relatively narrow spectral width, and cannot be used like mercury lamp illumination, a single illumination light source corresponds to a plurality of wave bands, 387 is only capable of adopting a plurality of 2 ED switching modes corresponding to multiband, and for a fluorescent microscope, a plurality of different wave bands are usually required to be equipped with B, G, V and UV4 wave bands.
2 bottoms of carousel are equipped with stop screw 16, base 1 corresponds stop screw 16 and is equipped with the arc storage tank, the arc storage tank limits stop screw 16 removal orbit, stop screw 16's effect prevents that fluorescence filter group 5 from changeing 8 positions of brush thimble and pushing up bad thimbles, L ED light source 4 can only rotate at brush limit semicircle and fluorescence filter group 5 can only rotate at brush contralateral semicircle when making carousel 2 rotate, according to the demand of different wave bands, only need appropriately adjust the position of illumination condensing lens 6, the focus can increase and decrease L ED light source 4 and fluorescence filter's group number with changing carousel 2 diameter, reach the optimization of device volume and how many of group number.
Still include shell 17, the shell 17 lateral part corresponds carousel 2 and is equipped with opening 18, and 2 edges of carousel stretch out opening 18, make things convenient for the operator to rotate carousel 2, and shell 17 still is equipped with upper and lower through-hole 19 corresponding to the fluorescence light path that dichroic mirror 10 passes through, and light jets out from the through-hole 19 on upper portion and gets into objective 24, and the through-hole 19 through the lower part forms an image.
Compared with the prior art, the utility model has the advantages of convenient to use saves space, does not generate heat and saves cost.
The utility model discloses can be equipped with L ED illumination light source and fluorescence filter group 5 of multiple colour, public illumination condensing lens 6 between each light source and the filter group, low cost, illumination brightness is high and distribute evenly, the monochromaticity is good, high lighting efficiency, L ED switching mode has advantages such as simpler (compare independent switching mode) before comparing, the cost is lower (compare common switching), compare traditional mercury lamp illumination and emit a large amount of heat, therefore the lamp house must have good space and heat dissipation condition, even so, the lamp house outside highest temperature all reaches more than 100 ℃, cause the maloperation very easily and touch the surface of generating heat and cause the scald, the utility model discloses fully consider the heat dissipation problem of whole L ED module, L ED module and whole carousel 2, illuminator shell 17 link to each other, thereby form good heat dissipation with the external world, the highest temperature when whole illuminator is worked can be controlled within +20 ℃ of room temperature.
The utility model discloses a illuminator can start at will and need not preheat, the cooling, realize switching at any time and closing, light source luminance is adjustable and stable in succession, solve traditional high-pressure mercury lamp and can not frequently start, the nonadjustable use defect of luminance, can effectively prolong the life of fluorescence sample simultaneously, consider from the security, there is not the lamp tube explosion phenomenon that mercury lamp pressure inequality caused, and simultaneously, for the xenon lamp, select corresponding spectral characteristic requirement L ED based on different micro-fluorescence dyestuff, the adaptation face is former stronger than the xenon lamp, and possess absolute advantage in the aspect of the price.
The life of the high-pressure mercury lamp is related to the starting times and the working time, the starting times are more, the life is shorter each time the working time is shorter, the high-pressure mercury lamp can be closed after at least 20 minutes after being started generally, the service life is only about 200 hours, when the mercury lamp emits light, 50-70 standard atmospheric pressures are generated in a bubble, therefore, the lamp must be placed in a firm lamp chamber, and in case of explosion, people cannot be injured, the normal service life of L ED can exceed 1 ten thousand hours, and the lamp does not need to be replaced in the end life compared with the service life of a microscope.
The utility model discloses a working method is:
l the light emitted from the ED light source 4 passes through the illumination condenser 6, the light passes through the excitation sheet 9 in the fluorescence filter set 5 after being converged, the excitation sheet 9 is generally a band-pass filter, the redundant wave band of the light source is cut off, the light passing through the excitation sheet 9 is reflected at the dichroic mirror 10 and then passes through the objective lens 24, and the objective lens 24 at this time belongs to a part of the illumination light path, so that the light can be uniformly illuminated on the focal plane.
The light irradiates the sample on the focal plane and then excites fluorescence, the wave peak of the excited fluorescence is not in the same wave band as the wave peak of the illuminating light, the excited fluorescence passes through the objective lens 24, at the moment, the objective lens 24 is a part of an imaging light path, then the light passes through the dichroic mirror 10, the dichroic mirror 10 plays a role in reflecting the illuminating light in the illuminating light path, and the imaging light passes through the imaging light in the imaging light path. Finally, the light passes through the stop sheet 11 to filter out unwanted wave bands, and then the final imaging is obtained in an eyepiece or a camera and the like.
Finally, it should be noted that: although the present invention has been described in detail with reference to the embodiments, it will be apparent to those skilled in the art that modifications, equivalents, improvements and the like can be made in the technical solutions of the foregoing embodiments or in some technical features of the foregoing embodiments, but all modifications, equivalents, improvements and the like within the spirit and principle of the present invention are intended to be included in the scope of the present invention.
Claims (7)
1. A rotary disc switching type multiband L ED fluorescent device is characterized by comprising a base, wherein the base is provided with a rotary disc, the rotary disc is circumferentially provided with at least two L ED light sources and at least two fluorescent filter sets, the L ED light sources and the fluorescent filter sets are arranged at opposite 180-degree positions, a lighting condenser lens is arranged at the center of the rotary disc, the base is provided with an electric brush mechanism corresponding to the L ED light sources, each fluorescent filter set comprises an excitation sheet, a dichroic mirror and a stop sheet, the stop sheet is horizontally arranged below the dichroic mirror, the dichroic mirror and the stop sheet form a certain angle, light rays sequentially pass through the excitation sheet, the dichroic mirror and the stop sheet, and the rotary disc switching type multiband L ED fluorescent device further comprises a device for limiting the rotation angle of the rotary disc.
2. The rotary switching multiband L ED fluorescent device of claim 1, wherein the base has a mounting hole, a spring and a positioning top bead on the top of the spring are disposed in the mounting hole, each L ED light source and fluorescent filter set forms a complete fluorescent lighting excitation set, and each set of working positions has a positioning groove corresponding to the positioning top bead.
3. The rotary switching multiband L ED fluorescent device of claim 1, wherein the brush mechanism has brush pins corresponding to the L ED light source.
4. The rotary switching multiband L ED fluorescent device of claim 1, wherein the means for limiting the rotation angle of the rotary disk comprises a limit screw and an arc-shaped receiving groove, the limit screw is disposed on the bottom of the rotary disk, the arc-shaped receiving groove is disposed on the base corresponding to the limit screw, and the arc-shaped receiving groove limits the moving track of the limit screw.
5. The turntable switched multiband L ED fluorescent device of claim 1, further comprising a housing, wherein the housing side is provided with an opening corresponding to the turntable, and the edge of the turntable protrudes out of the opening.
6. The rotating disk switching multiband L ED fluorescence device of claim 5, wherein the housing is provided with a through hole corresponding to the fluorescence path passed by the dichroic mirror.
7. The turntable-switched multiband L ED fluorescent device of claim 5, further comprising a fluorescent power controller connected to the brush mechanism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922320479.8U CN211149053U (en) | 2019-12-20 | 2019-12-20 | Turntable switching type multiband L ED fluorescent device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922320479.8U CN211149053U (en) | 2019-12-20 | 2019-12-20 | Turntable switching type multiband L ED fluorescent device |
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| CN211149053U true CN211149053U (en) | 2020-07-31 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113451872A (en) * | 2021-06-28 | 2021-09-28 | 长春理工大学 | Quick start intermediate infrared laser and corresponding polycrystal switching device |
| CN114414482A (en) * | 2022-01-11 | 2022-04-29 | 中国科学院南海海洋研究所 | Multispectral seawater wide-angle body light source for scattering function measurement |
| CN116719156A (en) * | 2023-06-30 | 2023-09-08 | 广州市明美光电技术有限公司 | Stereoscopic fluorescent microscope module |
-
2019
- 2019-12-20 CN CN201922320479.8U patent/CN211149053U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113451872A (en) * | 2021-06-28 | 2021-09-28 | 长春理工大学 | Quick start intermediate infrared laser and corresponding polycrystal switching device |
| CN113451872B (en) * | 2021-06-28 | 2022-08-09 | 长春理工大学 | Quick start intermediate infrared laser and corresponding polycrystal switching device |
| CN114414482A (en) * | 2022-01-11 | 2022-04-29 | 中国科学院南海海洋研究所 | Multispectral seawater wide-angle body light source for scattering function measurement |
| CN114414482B (en) * | 2022-01-11 | 2023-10-20 | 中国科学院南海海洋研究所 | Multispectral light source for measuring wide-angle sea water body scattering function |
| CN116719156A (en) * | 2023-06-30 | 2023-09-08 | 广州市明美光电技术有限公司 | Stereoscopic fluorescent microscope module |
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