CN211112000U

CN211112000U - A water bath device for microbiological examination

Info

Publication number: CN211112000U
Application number: CN201921234649.4U
Authority: CN
Inventors: 孔梅
Original assignee: TIANJIN CENTER FOR DISEASE CONTROL AND PREVENTION
Current assignee: TIANJIN CENTER FOR DISEASE CONTROL AND PREVENTION
Priority date: 2019-08-01
Filing date: 2019-08-01
Publication date: 2020-07-28
Anticipated expiration: 2029-08-01

Abstract

The utility model relates to the field of experimental instrument equipment, in particular to a water bath device for microorganism inspection, which comprises a main box, a main box cover, a water adding box and a water adding box cover; the main box is provided with an air inlet, the air inlet is provided with a sterilization layer, and the main box cover is provided with an air outlet; a main heat insulation baffle is arranged in the main tank and is divided into two independent water bath areas; a first rotary stirring component and a second rotary stirring component are respectively arranged in the two independent water bath areas; each single water bath area is provided with a heat insulation baffle plate and is divided into four water bath units; a filter layer is arranged in the water adding tank; the water tank and the main tank are connected through a first water circulating pipe and a second water circulating pipe; the water adding tank and the main tank are connected through a first water inlet pipe and a second water inlet pipe; the lower part of the water adding tank is provided with a first water discharging pipe and a second water discharging pipe. The utility model discloses can replace the air in the main tank, circulate and purify water, can carry out the water bath to the microorganism sample of different experimental conditions simultaneously.

Description

A water bath device for microbiological examination

Technical Field

The utility model belongs to the technical field of laboratory paraphernalia, especially, relate to a water bath device for microbiological examination.

Background

The water bath device is mainly used for distillation, drying and concentration in a laboratory, can also be used for constant temperature heating and other temperature tests, and is a necessary tool for biology, heredity, viruses, aquatic products, environmental protection, medicine, sanitation, laboratories, analysis rooms and educational scientific research.

In the microbiological examination industry, water bath heating is a method commonly used in the microbiological examination process at present, and the water bath heating means that water is added into a large container, then a reaction container (such as a culture dish, a beaker and the like) to be heated is put into the water, and heat is transferred into the reaction container to be heated by heating the water in the large container, so that the heating purpose is achieved.

When the water bath tank is used for heating in a water bath, the temperature accuracy of reactants in a reaction container to be heated can be ensured only when the reactants are lower than the water bath surface in the water bath tank, but the water bath in the microbial detection process has high requirement on data stability, and sometimes the requirement on the water bath atmosphere (such as oxygen-free atmosphere) is required, but many water bath tanks cannot control the water bath atmosphere at present, and finally the experimental test data of the heated substances are unstable. In addition, the current water bath tank can only carry out water bath on samples with the same experimental condition requirements at the same time, and can not carry out water bath on samples under different experimental conditions at the same time, so that the experimental efficiency is influenced, and the labor and time costs are increased.

Disclosure of Invention

In view of this, the utility model aims at providing a water bath for microbiological examination, can carry out the water bath to the microorganism sample that different experimental conditions required simultaneously, improve the stability of experimental efficiency and experimental data.

In order to achieve the above purpose, the technical scheme of the utility model is realized like this:

a water bath device for microbial detection comprises a main tank and a water adding tank; the top of the main box is provided with a main box cover, one side of the main box is provided with an air inlet, and a sterilization layer is arranged in the air inlet; the top of the main box cover is provided with an air outlet far away from the air inlet; a main heat insulation baffle is arranged in the main box, and the main heat insulation baffle divides the interior of the main box into two independent water bath areas which are symmetrically distributed left and right; in each single water bath area, a plurality of bases are arranged at the bottom, electric heating wires are welded on the side wall of the lower part of each single water bath area, a screen plate is arranged above each base, and a plurality of heat separating baffles with the height lower than that of the air inlet are vertically arranged on each screen plate; a first rotary stirring component and a second rotary stirring component are respectively arranged in the two independent water bath areas; each single water bath area is divided into four water bath units which are symmetrically distributed by the heat insulation baffle, and two groups of fixed spring assemblies and a temperature sensor which are symmetrically distributed are fixed on the screen plate in each water bath unit; the top of the water adding tank is provided with a water adding tank cover which is provided with a water adding funnel; a water adding tank heat insulation baffle is arranged in the water adding tank, and the inside of the water adding tank is divided into two water adding areas which are symmetrically distributed left and right by the water adding tank heat insulation baffle; the upper part of the water adding tank is connected with the upper part of the main tank through a first water circulating pipe and a second water circulating pipe which are arranged in parallel; the left end of the first water circulating pipe is communicated with a single water bath area close to the left in the main tank, and the right end of the first water circulating pipe is communicated with a water adding area close to the right in the water adding tank; the left end of the second water circulating pipe is communicated with a right independent water bath area in the main tank, and the right end of the second water circulating pipe is communicated with a left water adding area in the water adding tank; the two water adding areas in the water adding tank are both provided with a filtering layer, and the right end of the first water circulating pipe and the right end of the second water circulating pipe are both higher than the filtering layers; a first small water pump is arranged on the first water circulating pipe, and a second small water pump is arranged on the second water circulating pipe; the lower part of the water adding tank is connected with the lower part of the main tank through a first water inlet pipe and a second water inlet pipe which are arranged in parallel, the left end of the first water inlet pipe is communicated with a left water bath area in the main tank, the left end of the second water inlet pipe is communicated with a right water bath area in the main tank, and the right end of the first water inlet pipe and the right end of the second water inlet pipe are both communicated with the inside of the water adding tank; the lower part of the water adding tank is provided with a first water discharging pipe and a second water discharging pipe.

Further, the first rotary stirring assembly comprises a first motor, a first rotating shaft and a first stirring rod which are sequentially connected from top to bottom; the second rotary stirring assembly comprises a second motor, a second rotating shaft and a second stirring rod which are sequentially connected from top to bottom; the screen plate is provided with a screen plate hole; the first rotating shaft and the second rotating shaft penetrate through the heat insulation baffle plate from top to bottom and pass through the mesh plate holes; the first stirring rod and the second stirring rod are horizontally arranged, the center of the first stirring rod is connected with the lower end of the first rotating shaft, and the center of the second stirring rod is connected with the lower end of the second rotating shaft; the fixed spring assembly comprises a fixed block, a spring, a connecting block and an arc-shaped block which are welded in sequence; the inner surface of the arc block is provided with a friction layer.

Furthermore, the main box cover is hinged to the edge of the top of the main box through a hinge column, and the inner side of the main box cover is in close contact with the edge of the top of the main box; an air inlet cover is arranged outside the air inlet, and an air outlet cover is arranged outside the air outlet; a first water circulation electromagnetic valve is arranged on the first water circulation pipe, and a second water circulation electromagnetic valve is arranged on the second water circulation pipe; the left end of the first water circulating pipe and the left end of the second water circulating pipe respectively penetrate through the heat insulation baffle at different positions and extend to the position above the screen plate; a first water inlet electromagnetic valve is arranged on the first water inlet pipe; a second water inlet electromagnetic valve is arranged on the second water inlet pipe; a first water discharge electromagnetic valve is arranged on the first water discharge pipe; and a second water discharging electromagnetic valve is arranged on the second water discharging pipe.

Further, the inner walls of the main tank, the main tank cover, the water adding tank and the water adding tank cover are all provided with heat insulation layers; the bottom of the main box is provided with a plurality of symmetrically distributed support legs; a liquid level ruler with scale marks is arranged on the right side of the main box; the main box cover shell is made of transparent material; the lower part of the main box shell is provided with a stamping groove, and a power switch is arranged in the stamping groove.

Furthermore, the main box shell is provided with a control module, and the control module can realize a control function through the existing control program and the existing circuit; the main box shell is provided with a control module, and the control module can realize a control function through the existing control program and the existing circuit; the control module is provided with a display screen, two temperature adjusting buttons, two heating lamps, two heat preservation lamps and a plurality of component control buttons; through current procedure and current circuit with display screen, two button that adjust the temperature, two heat lamps, two heat preservation lamps, a plurality of part control button and temperature sensor, heating wire, first motor, second motor, first hydrologic cycle solenoid valve, second hydrologic cycle solenoid valve, first small-size water pump, first solenoid valve of intaking, the small-size water pump of second, the solenoid valve is intake to the second, first solenoid valve of draining, the second solenoid valve of draining are connected.

Compared with the prior art, the water bath device for the microorganism inspection has the following advantages:

(1) the main box of the utility model is internally provided with the main heat insulation baffle, and the main box is divided into two independent water bath areas which are symmetrically distributed, so that water bath can be carried out on two groups of samples which need different experimental conditions;

(2) the air inlet is arranged in the main box, the air inlet is provided with the sterilization layer, the air outlet is arranged at the top of the main box cover far away from the air inlet, the air in the main box can be replaced,

the interference of air to microorganisms in the culture container in the water bath process is avoided, and the stability of data is ensured;

(3) the utility model is provided with a first rotary stirring component and a second rotary stirring component in different independent water bath areas in the main box, which can stir water and avoid the problem of uneven water temperature caused by heating by the electric heating wire;

(4) the utility model is provided with the first water circulation pipeline, the second water circulation pipeline, the first water inlet pipeline and the second water inlet pipeline, can further ensure the mobility of water used in water bath through the circulation of water in the water bath process, has synergistic effect with the rotary stirring component and ensures the uniform temperature of the water bath;

(5) the utility model is divided into a main tank and a water adding tank, which is convenient for disassembly and maintenance;

(6) the main box shell of the utility model is provided with a control module, and the control module is connected with corresponding parts through the existing circuit and the existing program, so that the intelligent control is realized;

(7) the utility model discloses set up the filter layer in the water tank, can purify water, prevent the main tank and add the interior foster bacterium of water tank.

Drawings

The accompanying drawings, which form a part hereof, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention without undue limitation. In the drawings:

FIG. 1 is a schematic front view of a water bath apparatus for microbiological examination according to an embodiment of the present invention;

FIG. 2 is a schematic top view of a water bath apparatus for microbiological examination according to an embodiment of the present invention;

fig. 3 is a schematic structural view of the fixing spring assembly of the present invention;

FIG. 4 is a schematic view of a front view of the housing of the water bath device for microbial testing according to the present invention;

fig. 5 is a schematic view of the structure of the net plate of the present invention.

Description of reference numerals:

1-a main box; 2-main box cover; 3-adding a water tank; 4-a funnel; 5-a support leg; 6-hinged columns; 7-an air inlet cover; 8-air outlet cover; 9-a first small water pump; 10-a first water circulation pipe; 11-a liquid level ruler; 12-a first water inlet pipe; 13-a first water inlet solenoid valve; 14-a second water inlet pipe; 15-a second water inlet solenoid valve; 16-a first water discharge pipe; 17-a first water discharge solenoid valve; 18-a first water circulation solenoid valve; 19-a second water circulation solenoid valve; 20-a primary insulation barrier; 21-a base; 22-a first motor; 23-a first shaft; 24-a first stirring rod; 25-a second motor; 26-a second shaft; 27-a second stirring rod; 28-mesh plate; 29-electric heating wire; 30-a fixed spring assembly; 301-fixing block; 302-a spring; 303-connecting block; 304-an arc-shaped block; 305-a friction layer; 32-a temperature sensor; 33-separating thermal barriers; 34-a filter layer; 35-an air inlet; 36-a degerming layer; 37-air outlet; 38-heat insulation layer; 39-a water tank cover; 40-mesh holes; 41-stamping a groove; 42-a power switch; 43-a display screen; 44-temperature adjustment button; 45-heating lamps; 46-a heat preservation lamp; 47-part control button; 48-a second water circulation pipe; 49-a second small water pump; 50-water tank heat insulation baffle; 51-a second water discharge pipe; 52-second water discharge electromagnetic valve.

Detailed Description

It should be noted that, in the present invention, the embodiments and features of the embodiments may be combined with each other without conflict.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are used merely for convenience of description and for simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.

The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.

Referring to fig. 1 to 5, a water bath apparatus for microbiological examination includes a main tank 1, a water adding tank 3; the top of the main box 1 is provided with a main box cover 2, one side of the main box 1 is provided with an air inlet 35, and a sterilization layer 36 is arranged in the air inlet 35; the top of the main box cover 2 is provided with an air outlet 37 far away from the air inlet 35; a main heat insulation baffle 20 is arranged in the main box 1, and the main heat insulation baffle 20 divides the interior of the main box 1 into two independent water bath areas which are symmetrically distributed left and right; in each single water bath area, a plurality of bases 21 are arranged at the bottom, heating wires 29 are welded on the side wall of the lower part of each single water bath area, a screen plate 28 is arranged above each base 21, and a plurality of sub heat insulation baffle plates 33 with the height lower than that of an air inlet 35 are vertically arranged on each screen plate 28; a first rotary stirring component and a second rotary stirring component are respectively arranged in the two independent water bath areas; the separate heat baffle 33 divides each single water bath into a plurality of water bath units which are symmetrically distributed, preferably four water bath units, and two groups of fixed spring assemblies 30 and a temperature sensor 32 which are symmetrically distributed are fixed on the screen plate 28 in each water bath unit; the top of the water adding tank 3 is provided with a water adding tank cover 39, and the water adding tank cover 39 is provided with a water adding funnel 4; a water adding tank heat insulation baffle 50 is arranged in the water adding tank 3, and the inside of the water adding tank 3 is divided into two water adding areas which are symmetrically distributed left and right by the water adding tank heat insulation baffle 50; the upper part of the feed water tank 3 is connected to the upper part of the main tank 1 through a first water circulation pipe 10 and a second water circulation pipe 48 which are arranged in parallel; the left end of the first water circulating pipe 10 is communicated with a single water bath area close to the left in the main tank 1, and the right end of the first water circulating pipe 10 is communicated with a water adding area close to the right in the water adding tank 3; the left end of the second water circulating pipe 48 is communicated with a single water bath area close to the right in the main tank 1, and the right end of the second water circulating pipe 48 is communicated with a water adding area close to the left in the water adding tank 3; the two water adding areas in the water adding tank 3 are both provided with a filter layer 34, and the right end of the first water circulating pipe 10 and the right end of the second water circulating pipe 48 are both higher than the filter layer 34; a first small water pump 9 is arranged on the first water circulating pipe 10, and a second small water pump 49 is arranged on the second water circulating pipe 48; the lower part of the water adding tank 3 is connected with the lower part of the main tank 1 through a first water inlet pipe 12 and a second water inlet pipe 14 which are arranged in parallel, the left end of the first water inlet pipe 12 is communicated with a water bath area close to the left in the main tank 1, the left end of the second water inlet pipe 14 is communicated with a water bath area close to the right in the main tank 1, and the right end of the first water inlet pipe 12 and the right end of the second water inlet pipe 14 are both communicated with the inside of the water adding tank 3; the lower part of the water adding tank 3 is provided with a first water discharging pipe 16 and a second water discharging pipe 51.

The first rotary stirring assembly comprises a first motor 22, a first rotating shaft 23 and a first stirring rod 24 which are connected in sequence from top to bottom; the second rotary stirring component comprises a second motor 25, a second rotating shaft 26 and a second stirring rod 27 which are connected in sequence from top to bottom; mesh plate holes 40 are formed in the mesh plate 28; the first rotating shaft 23 and the second rotating shaft 26 pass through the mesh holes 40 from top to bottom; the first stirring rod 24 and the second stirring rod 27 are both horizontally arranged, the center of the first stirring rod 24 is connected with the lower end of the first rotating shaft 23, and the center of the second stirring rod 27 is connected with the lower end of the second rotating shaft 26; the fixed spring assembly 30 comprises a fixed block 301, a spring 302, a connecting block 303 and an arc-shaped block 304 which are welded in sequence; the inner surface of the arc-shaped block 304 is provided with a friction layer 305.

The main box cover 2 is hinged on the top edge of the main box 1 through a hinge column 6, and the inner side of the main box cover 2 is in close contact with the top edge of the main box 1. An air inlet cover 7 is arranged outside the air inlet 35, and an air outlet cover 8 is arranged outside the air outlet 37.

A first water circulation solenoid valve 18 is arranged on the first water circulation pipe 10, and a second water circulation solenoid valve 19 is arranged on the second water circulation pipe 48; the left end of the first water circulation pipe 10 and the left end of the second water circulation pipe 48 respectively pass through the heat insulation barrier 33 at different positions to extend up to above the mesh plate 28. A first water inlet electromagnetic valve 13 is arranged on the first water inlet pipe 12; a second water inlet electromagnetic valve 15 is arranged on the second water inlet pipe 14; a first water discharge electromagnetic valve 17 is arranged on the first water discharge pipe 16; the second water discharge pipe 51 is provided with a second water discharge electromagnetic valve 52.

The inner walls of the main tank 1, the main tank cover 2, the water adding tank 3 and the water adding tank cover 39 are all provided with a heat insulation layer 38; the bottom of the main box 1 is provided with a plurality of supporting legs 5 which are symmetrically distributed; a liquid level ruler 11 with scale marks is arranged on the right side of the main box; the main case cover 2 is made of transparent material.

A stamping groove 42 is formed in the lower portion of the shell of the main box 1, and a power switch 43 is mounted in the stamping groove 42 and can be prevented from being touched by mistake; the shell of the main box 1 is provided with a control module, and the control module can realize a control function through the existing control program and the existing circuit; the control module is provided with a display screen 43, two temperature adjusting buttons 44, two heating lamps 45, two heat preservation lamps 46 and a plurality of component control buttons 47; the display screen 43, the two temperature adjusting buttons 44, the two heating lamps 45, the two heat preservation lamps 46, the plurality of component control buttons 47, the temperature sensor 32, the heating wire 29, the first motor 22, the second motor 25, the first water circulation solenoid valve 18, the second water circulation solenoid valve 19, the first small water pump 9, the first water inlet solenoid valve 13, the second small water pump 49, the second water inlet solenoid valve 15, the first water discharge solenoid valve 17 and the second water discharge solenoid valve 52 are connected through an existing program and an existing circuit.

According to the figures 1-5, the water bath process is divided into four stages of gas replacement, water addition, temperature control and circulation.

In the gas replacement stage, clean air or other gases (such as nitrogen, argon and the like) are used for replacing the original air in the main box 1, so that the interference of the air on microorganisms is avoided. Firstly, opening a main box cover 2, and sequentially placing culture containers filled with microorganisms on a screen plate 28, wherein each culture container is positioned in a water bath unit; under the condition that the spring 302 generates elastic deformation, the culture container can be firmly clamped by the arc-shaped block 304, so that the phenomenon that the container is toppled due to the influence of water buoyancy in the water bath process is avoided. The main box cover 2 at the top of the main box 1 is closed, the air inlet cover 7 is opened, the air outlet cover 8 is opened, and the gas used for replacement is introduced into the air inlet 35, and driven by the pressure, the gas firstly passes through the degerming layer 36 in the air inlet 35 and then enters the main box 1, and the original air in the main box 1 is discharged through the air outlet 37; with the introduction of the gas, the entire volume of the original air in the main tank 1 is finally replaced with the used gas, and the inlet cover 7 and the outlet cover 8 are closed.

In the stage of adding water, water is poured into the funnel 4 at the top of the water adding tank 3, the water flows downwards along the funnel 4 and flows into two different water adding areas in the water adding tank 3, impurities are removed through the filter layer 34, and at the moment, the first water inlet electromagnetic valve 13, the second water inlet electromagnetic valve 15, the first water outlet electromagnetic valve 17 and the second water outlet electromagnetic valve 52 are all in a closed state. A first water inlet electromagnetic valve 13 on a first water inlet pipe 12 is opened, and water in a left water adding area in the water adding tank 3 enters a right independent water bath area in the main tank 1 through the first water inlet pipe 12; a second water inlet electromagnetic valve 15 on a second water inlet pipe 14 is opened, and water in a right water adding area in the water adding tank 3 enters a left independent water bath area in the main tank 1 through the second water inlet pipe 14; the liquid level of the water in the main tank 1 is judged through a liquid level ruler 11 on the main tank 1; the liquid level needs to be higher than the screen plate 28 and ensures that all microorganisms in the microorganism culture container are below the liquid level, and the liquid level cannot exceed the highest position of the microorganism culture container; because the main box cover 2 is made of transparent material, the position of the liquid level can be directly observed by naked eyes; when the liquid level required by the water bath is reached, the first water inlet electromagnetic valve 13 and the second water inlet electromagnetic valve 15 are closed.

In the temperature control stage, the temperature is adjusted through a temperature adjusting button 45 on a shell control module of the main box 1, a heating lamp 46 is turned on, and the heating wire 29 heats water in the main box 1; when the water temperature reaches the set temperature, the heat-retaining lamp 47 lights up. When the heating wire 29 heats water, the first motor 22 is turned on, and the first motor 22 drives the first stirring rod 24 to rotate through the first rotating shaft 23; turning on the second motor 25, the second motor 25 drives the second stirring rod 27 to rotate through the second rotating shaft 26; the rotation of the stirring rod can ensure that the microorganisms in the culture container are heated uniformly.

During the circulation phase, when the water temperature has reached the water bath requirement, the purpose of the circulation is to further promote the flow of water in the water reservoir 1. Open first water circulation solenoid valve 18, open second water circulation solenoid valve 19, open first small-size water pump 9, open second small-size water pump 49, open first water solenoid valve 13, open second water solenoid valve 15, under the effect of first small-size water pump 9 and second small-size water pump 49, water in two independent water bath district in the main tank 1 can be pumped to adding in the water tank 3, and the water in adding the water tank 3 gets into the independent water bath district that leans on the right and the independent water bath district that leans on the left in the main tank 1 respectively through first inlet tube 12 and second inlet tube 14, just so realized the circulation flow of water bath in-process water. And after the water bath is finished, closing the first water circulation electromagnetic valve 18, the second water circulation electromagnetic valve 19, the first small water pump 9, the second small water pump 49, the first water inlet electromagnetic valve 13 and the second water inlet electromagnetic valve 15.

In the whole process of the water bath, the temperature of each water bath unit can be displayed on the display screen 43 through the existing program and the existing circuit, the first motor 22, the second motor 25, the first water circulation electromagnetic valve 18, the second water circulation electromagnetic valve 19, the first small water pump 9, the first water inlet electromagnetic valve 13, the second small water pump 49, the second water inlet electromagnetic valve 15, the first water discharge electromagnetic valve 17 and the second water discharge electromagnetic valve 52 can be controlled to be opened and closed through the component control button 47, and the operation of experimenters is facilitated.

The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A water bath device for microbiological examination, characterized in that: the water-saving device comprises a main box (1) and a water adding box (3); the top of the main box (1) is provided with a main box cover (2), one side of the main box (1) is provided with an air inlet (35), and the air inlet (35) is internally provided with a sterilization layer (36); an air outlet (37) is arranged at the top of the main box cover (2) far away from the air inlet (35); a main heat insulation baffle (20) is arranged in the main box (1), and the main heat insulation baffle (20) divides the interior of the main box (1) into two independent water bath areas which are symmetrically distributed left and right; in each single water bath area, a plurality of bases (21) are arranged at the bottom, heating wires (29) are welded on the side wall of the lower part of each single water bath area, a screen plate (28) is arranged above each base (21), and a plurality of separated heat baffles (33) with the height lower than that of the air inlet (35) are vertically arranged on each screen plate (28); a first rotary stirring component and a second rotary stirring component are respectively arranged in the two independent water bath areas; each single water bath area is divided into four water bath units which are symmetrically distributed by a separating heat baffle (33), and two groups of fixed spring assemblies (30) which are symmetrically distributed and a temperature sensor (32) are fixed on the screen plate (28) in each water bath unit; a water adding tank cover (39) is arranged at the top of the water adding tank (3), and a water adding funnel (4) is arranged on the water adding tank cover (39); a water adding tank heat insulation baffle (50) is arranged in the water adding tank (3), and the inside of the water adding tank (3) is divided into two water adding areas which are symmetrically distributed left and right by the water adding tank heat insulation baffle (50); the upper part of the water adding tank (3) is connected with the upper part of the main tank (1) through a first water circulating pipe (10) and a second water circulating pipe (48) which are arranged in parallel; the left end of the first water circulating pipe (10) is communicated with a single water bath area close to the left in the main tank (1), and the right end of the first water circulating pipe (10) is communicated with a water adding area close to the right in the water adding tank (3); the left end of the second water circulating pipe (48) is communicated with a single water bath area close to the right in the main tank (1), and the right end of the second water circulating pipe (48) is communicated with a water adding area close to the left in the water adding tank (3); the two water adding areas in the water adding tank (3) are both provided with a filter layer (34), and the right end of the first water circulating pipe (10) and the right end of the second water circulating pipe (48) are both higher than the filter layer (34); a first small water pump (9) is arranged on the first water circulating pipe (10), and a second small water pump (49) is arranged on the second water circulating pipe (48); the lower part of the water adding tank (3) is connected with the lower part of the main tank (1) through a first water inlet pipe (12) and a second water inlet pipe (14) which are arranged in parallel, the left end of the first water inlet pipe (12) is communicated with a left water bath area in the main tank (1), the left end of the second water inlet pipe (14) is communicated with a right water bath area in the main tank (1), and the right end of the first water inlet pipe (12) and the right end of the second water inlet pipe (14) are both communicated with the inside of the water adding tank (3); the lower part of the water adding tank (3) is provided with a first water discharging pipe (16) and a second water discharging pipe (51).

2. The water bath apparatus for microbiological examination as claimed in claim 1, wherein: the first rotary stirring assembly comprises a first motor (22), a first rotating shaft (23) and a first stirring rod (24) which are sequentially connected from top to bottom; the second rotary stirring component comprises a second motor (25), a second rotating shaft (26) and a second stirring rod (27) which are connected in sequence from top to bottom; mesh plate holes (40) are formed in the mesh plate (28); the first rotating shaft (23) and the second rotating shaft (26) penetrate through the heat insulation baffle plate (33) from top to bottom and pass through the mesh plate holes (40); the first stirring rod (24) and the second stirring rod (27) are both horizontally placed, the center of the first stirring rod (24) is connected with the lower end of the first rotating shaft (23), and the center of the second stirring rod (27) is connected with the lower end of the second rotating shaft (26).

3. The water bath apparatus for microbiological examination as claimed in claim 1, wherein: the fixed spring assembly (30) comprises a fixed block (301), a spring (302), a connecting block (303) and an arc-shaped block (304) which are welded in sequence; the inner surface of the arc-shaped block (304) is provided with a friction layer (305).

4. The water bath apparatus for microbiological examination as claimed in claim 1, wherein: the main box cover (2) is hinged on the top edge of the main box (1) through a hinge column (6), and the inner side of the main box cover (2) is in close contact with the top edge of the main box (1).

5. The water bath apparatus for microbiological examination as claimed in claim 1, wherein: an air inlet cover (7) is arranged outside the air inlet (35), and an air outlet cover (8) is arranged outside the air outlet (37).

6. The water bath apparatus for microbiological examination as claimed in claim 1, wherein: a first water circulation electromagnetic valve (18) is arranged on the first water circulation pipe (10), and a second water circulation electromagnetic valve (19) is arranged on the second water circulation pipe (48); the left end of the first water circulation pipe (10) and the left end of the second water circulation pipe (48) respectively pass through the heat insulation baffle (33) at different positions and extend to the position above the screen plate (28).

7. The water bath apparatus for microbiological examination as claimed in claim 1, wherein: a first water inlet electromagnetic valve (13) is arranged on the first water inlet pipe (12); a second water inlet electromagnetic valve (15) is arranged on the second water inlet pipe (14); a first water discharging electromagnetic valve (17) is arranged on the first water discharging pipe (16); and a second water discharging electromagnetic valve (52) is arranged on the second water discharging pipe (51).

8. The water bath apparatus for microbiological examination as claimed in claim 1, wherein: the inner walls of the main tank (1), the main tank cover (2), the water adding tank (3) and the water adding tank cover (39) are all provided with heat insulation layers (38); a plurality of supporting legs (5) which are symmetrically distributed are arranged at the bottom of the main box (1); a liquid level ruler (11) with scale marks is arranged on the right side of the main box (1); the shell of the main box cover (2) is made of transparent material.

9. The water bath apparatus for microbiological examination as claimed in claim 1, wherein: the lower part of the shell of the main box (1) is provided with a stamping groove (41), and a power switch (42) is installed in the stamping groove (41).

10. The water bath apparatus for microbiological examination as claimed in claim 1, wherein: the shell of the main box (1) is provided with a control module, and the control module can realize a control function through the existing control program and the existing circuit; the control module is provided with a display screen (43), two temperature adjusting buttons (44), two heating lamps (45), two heat preservation lamps (46) and a plurality of component control buttons (47); a display screen (43), two temperature adjusting buttons (44), two heating lamps (45), two heat preservation lamps (46), a plurality of component control buttons (47), a temperature sensor (32), an electric heating wire (29), a first motor (22), a second motor (25), a first water circulation electromagnetic valve (18), a second water circulation electromagnetic valve (19), a first small water pump (9), a first water inlet electromagnetic valve (13), a second small water pump (49), a second water inlet electromagnetic valve (15), a first water drain electromagnetic valve (17) and a second water drain electromagnetic valve (52) are connected through an existing program and an existing circuit.