CN211043408U - Combined detection device for transferrin, hemoglobin and helicobacter pylori antigen - Google Patents
Combined detection device for transferrin, hemoglobin and helicobacter pylori antigen Download PDFInfo
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- CN211043408U CN211043408U CN201921038888.2U CN201921038888U CN211043408U CN 211043408 U CN211043408 U CN 211043408U CN 201921038888 U CN201921038888 U CN 201921038888U CN 211043408 U CN211043408 U CN 211043408U
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- transferrin
- hemoglobin
- test strip
- detection
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Abstract
The utility model relates to a combined detection device for transferrin, hemoglobin and helicobacter pylori antigens. The combined detection device comprises an upper shell (1), a lower shell (2) and detection test strip components (3, 4), wherein the detection test strip components (3, 4) are arranged between the upper shell (1) and the lower shell (2) and are used for simultaneously detecting the transferrin, the hemoglobin and the helicobacter pylori antigen. The utility model discloses a combined detection device of transferrin, hemoglobin and helicobacter pylori antigen can detect transferrin, hemoglobin and helicobacter pylori antigen simultaneously.
Description
Technical Field
The utility model relates to an external diagnosis technical field especially relates to a combined detection device of transferrin, hemoglobin and helicobacter pylori antigen.
Background
The detection of occult blood in stool has important value for the diagnosis of chronic gastrointestinal hemorrhage and the screening of malignant large intestine tumor. The indexes of the occult blood of the excrement detected by the conventional immunoassay method are hemoglobin and transferrin, and the hemoglobin and the transferrin in the excrement can be simultaneously detected by applying a combined immunoassay method, so that the positive detection rate of digestive tract hemorrhagic diseases is obviously improved.
Helicobacter pylori is a gram-negative microaerophilic bacterium which is parasitic in the stomach and duodenum, and its infection is very common, with a global natural population infection rate of more than 50%, and the route of transmission is presumed to be oral infection.
Helicobacter pylori infection is an infectious disease, and can cause chronic gastritis, peptic ulcer and the like, common symptoms comprise discomfort of the upper stomach, pain, flatulence, anorexia, nausea, vomiting, dark or tar-colored feces and the like, and more than 70 percent of infected people have no obvious symptoms. Helicobacter pylori is a main pathogenic factor of gastritis and peptic ulcer, is closely related to the occurrence of functional dyspepsia, gastric mucosa-associated lymphoblastic lymphoma and gastric cancer, and is listed as a class I carcinogenic factor by the international cancer research organization of the world health organization.
Eradication of H.pylori can significantly reduce the incidence of gastric and duodenal diseases, and can reduce new cases of H.pylori infection in the future. At present, the gold standard for detecting helicobacter pylori is a bacteria isolation culture technology, but the gold standard is not easy to popularize due to high experimental conditions and technical requirements. And the detection of the helicobacter pylori antigen in the excrement does not need expensive equipment, is convenient to operate and interpret results, has good sensitivity and specificity, and is increasingly popularized.
At present, the fecal detection device is provided with a combined detection device for transferrin and hemoglobin, but a combined detection device capable of simultaneously detecting transferrin, hemoglobin and helicobacter pylori antigens is lacked.
SUMMERY OF THE UTILITY MODEL
Based on the above-mentioned defect among the prior art, the utility model aims to provide a can detect jointly detection device of transferrin, hemoglobin and helicobacter pylori antigen simultaneously.
Therefore, the utility model provides the following technical scheme.
The utility model provides a combined detection device of transferrin, hemoglobin and helicobacter pylori antigen, which comprises an upper shell, a lower shell and a detection test strip component,
the detection test strip component is arranged between the upper shell and the lower shell and is used for simultaneously detecting the transferrin, the hemoglobin and the helicobacter pylori antigen.
In at least one embodiment, the test strip assembly comprises a transferrin and hemoglobin combination test strip and a helicobacter pylori antigen test strip,
the transferrin and hemoglobin combined test strip is used for detecting the transferrin and the hemoglobin,
the test paper strip for detecting the helicobacter pylori antigen is used for detecting the helicobacter pylori antigen.
In at least one embodiment, the transferrin and hemoglobin combination test strip comprises a first bottom plate, a first water absorbent plate, a first nitrocellulose membrane, a first sample liquid absorbing layer, and a first color particle storage pad containing transferrin monoclonal antibody and hemoglobin monoclonal antibody,
and the first nitrocellulose membrane is loaded with a transferrin monoclonal antibody detection line, a hemoglobin monoclonal antibody detection line and a goat anti-mouse polyclonal antibody control line which are separated from each other.
In at least one embodiment, the test strip components include a transferrin test strip, a hemoglobin test strip, and a helicobacter pylori antigen test strip,
the transferrin detection test strip is used for detecting the transferrin,
the hemoglobin detection test strip is used for detecting the hemoglobin,
the test paper strip for detecting the helicobacter pylori antigen is used for detecting the helicobacter pylori antigen.
In at least one embodiment, the transferrin detection test strip comprises a third bottom plate, a third water absorption plate, a third nitrocellulose membrane, a third sample liquid absorption layer and a third color particle storage pad containing transferrin monoclonal antibodies, wherein the third nitrocellulose membrane carries transferrin monoclonal antibodies detection lines and goat anti-mouse polyclonal antibodies control lines which are separated from each other.
In at least one embodiment, the hemoglobin detection test strip comprises a fourth bottom plate, a fourth water absorption plate, a fourth nitrocellulose membrane, a fourth sample liquid absorption layer and a fourth color particle storage pad containing hemoglobin monoclonal antibodies, wherein the hemoglobin monoclonal antibodies detection line and the goat anti-mouse polyclonal antibody control line are separated from each other and are loaded on the fourth nitrocellulose membrane.
In at least one embodiment, the test strip for detecting helicobacter pylori antigen comprises a second bottom plate, a second water absorption plate, a second nitrocellulose membrane, a second sample liquid absorption layer and a second color particle storage pad containing the monoclonal antibody of helicobacter pylori antigen and the conjugate of biotin-bovine serum albumin,
the second nitrocellulose membrane is loaded with a helicobacter pylori antigen monoclonal antibody detection line and a goat anti-mouse polyclonal antibody and streptavidin control line which are separated from each other.
In at least one embodiment, the upper housing is provided with a sample input aperture and a viewing window,
the sample input hole is used for the sample to be detected to pass through and be loaded,
the observation window is arranged opposite to the detection test strip component in the thickness direction of the combined detection device and is used for observing the detection result of the detection test strip component.
In at least one embodiment, the lower housing is provided with a mounting slot in which the test strip assembly is disposed.
In at least one embodiment, the mounting slot is a rectangular slot.
Through adopting the technical scheme, the utility model provides a combined detection device of transferrin, hemoglobin and helicobacter pylori antigen can detect transferrin, hemoglobin and helicobacter pylori antigen simultaneously through setting up the detection test paper strip subassembly that can detect transferrin, hemoglobin and helicobacter pylori antigen simultaneously.
Drawings
Fig. 1 shows a schematic structural diagram of a first embodiment of the combined detection device for transferrin, hemoglobin and helicobacter pylori antigen according to the present invention.
Fig. 2 shows a schematic structural diagram of the transferrin and hemoglobin combined test strip in fig. 1.
Fig. 3 shows a cross-sectional view of fig. 2.
FIG. 4 shows a schematic structural diagram of the test strip for helicobacter pylori antigen detection in FIG. 1.
Fig. 5 shows a cross-sectional view of fig. 4.
Fig. 6 shows a schematic structural diagram of a second embodiment of the combined detection device for transferrin, hemoglobin and helicobacter pylori antigen according to the present invention.
Description of the reference numerals
1, an upper shell; 11 an upper shell body; 12 sample input aperture; 13 an observation window;
2, a lower shell; 21 a lower case body; 22, mounting grooves;
3 detecting the test strip component; 31, a combined test strip for detecting transferrin and hemoglobin; 311 a first base plate; 312 first water absorption plate; 313 a first nitrocellulose membrane; 3131 detecting transferrin monoclonal antibody; 3132 detecting the hemoglobin monoclonal antibody; 3133 goat anti-mouse polyclonal antibody control lines; 314 first sample wicking layer; 315 a first color particle storage pad; 32 helicobacter pylori antigen detection test paper; 321 a second base plate; 322 second suction plate; 323 a second nitrocellulose membrane; 3231 detecting helicobacter pylori antigen monoclonal antibody; 3232 goat anti-mouse polyclonal antibody, streptavidin control line; 324 a second sample pipette layer; 325 second color particle storage pad;
4 detecting the test strip component; 41 transferrin test strip; 42 hemoglobin detection test strip; 43 helicobacter pylori antigen test strip.
Detailed Description
Exemplary embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood that the detailed description is only intended to teach one skilled in the art how to practice the invention, and is not intended to exhaust all possible ways of practicing the invention, nor is it intended to limit the scope of protection of the invention.
(first embodiment)
The first embodiment of the combined detection device for transferrin, hemoglobin and helicobacter pylori antigen according to the present invention is described in detail below with reference to fig. 1 to 5.
In this embodiment, as shown in fig. 1, the combined detection device for transferrin, hemoglobin and helicobacter pylori antigen according to the present invention comprises an upper casing 1, a lower casing 2 and a detection test strip assembly 3. Wherein, detection test paper strip subassembly 3 sets up between last casing 1 and casing 2 down, goes up casing 1 and casing 2 connection down.
In the present embodiment, as shown in fig. 1, the upper case 1, which is rectangular as a whole, includes an upper case body 11, a sample input hole 12, and an observation window 13. The sample input hole 12 is used for adding a sample to be detected, and the observation window 13 is used for observing the detection result of the detection test strip component 3.
The sample input hole 12 is provided through the upper case body 11 in the thickness direction of the upper case body 11. The sample input holes 12 are circular holes, and the number of the sample input holes is two, and the two sample input holes 12 are arranged in parallel in the width direction of the upper case body 11.
The observation window 13 is arranged to penetrate through the upper shell body 11 along the thickness direction of the upper shell body 11, and the observation window 13 is arranged opposite to the detection test strip component 3 in the thickness direction of the combined detection device. The two observation windows 13 are arranged in parallel in the width direction of the upper case body 11.
In the present embodiment, as shown in fig. 1, the lower case 2, which is rectangular as a whole, includes a lower case body 21 and a mounting groove 22.
The mounting groove 22 is opened along the length direction of the lower case body 21. Wherein, the mounting grooves 22 are rectangular, the number thereof is two, and the two mounting grooves 22 are arranged in parallel along the width direction of the lower case body 21. The mounting slot 22 is used for mounting the test strip assembly 3 and accommodating the excess sample.
In the present embodiment, as shown in FIG. 1, the test strip assembly 3 includes a transferrin and hemoglobin combination test strip 31 and a helicobacter pylori antigen test strip 32. Wherein, the transferrin and hemoglobin combined test strip 31 and the helicobacter pylori antigen test strip 32 are respectively arranged in the corresponding mounting grooves 22.
In this embodiment, as shown in fig. 2 and 3, the transferrin and hemoglobin combined test strip 31 comprises a first bottom plate 311, a first water absorbent sheet 312, a first nitrocellulose membrane 313, a first sample liquid absorbent layer 314, and a first color particle storage pad 315 containing transferrin monoclonal antibody and hemoglobin monoclonal antibody.
The first water absorption plate 312 and the first sample liquid absorption layer 314 are respectively disposed at two ends of the first bottom plate 311, two ends of the first nitrocellulose membrane 313 are respectively connected to the first water absorption plate 312 and the first color particle storage pad 315, one end of the first sample liquid absorption layer 314 is connected to the first color particle storage pad 315, and the first nitrocellulose membrane 313 carries a transferrin monoclonal antibody detection line 3131, a hemoglobin monoclonal antibody detection line 3132, and a goat anti-mouse polyclonal antibody control line 3133, which are separated from each other.
In the present embodiment, as shown in fig. 4 and 5, the test strip 32 for detecting helicobacter pylori antigen comprises a second bottom plate 321, a second water absorption plate 322, a second nitrocellulose membrane 323, a second sample liquid absorption layer 324, and a second colored particle storage pad 325 containing a conjugate of helicobacter pylori antigen monoclonal antibody and biotin-BSA (Bovine Serum Albumin).
The second water absorption plate 322 and the second sample liquid absorption layer 324 are respectively arranged at two ends of the second bottom plate 321, two ends of the second nitrocellulose membrane 323 are respectively connected with the second water absorption plate 322 and the second color particle storage pad 325, one end of the second sample liquid absorption layer 324 is connected with the second color particle storage pad 325, and the second nitrocellulose membrane 323 is loaded with a helicobacter pylori antigen monoclonal antibody detection line 3231, a goat anti-mouse polyclonal antibody and a streptavidin control line 3232 which are separated from each other.
In the present embodiment, the color particles may be colloidal gold particles, and accordingly, the first and second color particle storage pads 315 and 325 may be gold-labeled pads.
The operation of the device for the combined detection of transferrin, hemoglobin and helicobacter pylori antigen according to the present invention will be described below.
In the present embodiment, when it is desired to detect a sample to be detected (for example, feces dissolved in a solution), the sample to be detected may be added from the sample input hole 12. The sample to be detected contacts the sample liquid absorbing layer firstly, and due to the capillary action, the sample to be detected can move towards the water absorbing plate along the length direction of the test paper.
During the movement, the sample to be detected is brought into contact with the respective detection and control lines, so that the detection and/or control lines are colored (e.g., red). The inspector can observe the detection result through the observation window 13.
Specifically, for the test strip 31 for combined detection of transferrin and hemoglobin, if only the goat anti-mouse polyclonal antibody control line 3133 is developed, it indicates that the content of transferrin and hemoglobin in the sample to be detected does not exceed the predetermined value, and the detection results of transferrin and hemoglobin are negative.
If the transferrin monoclonal antibody detection line 3131 and the goat anti-mouse polyclonal antibody control line 3133 are developed, the content of transferrin in the sample to be detected exceeds a preset value, the detection result of transferrin is positive, and the detection result of hemoglobin is negative.
If the hemoglobin monoclonal antibody detection line 3132 and the goat anti-mouse polyclonal antibody control line 3133 are developed, it is indicated that the content of hemoglobin in the sample to be detected exceeds a predetermined value, the detection result of hemoglobin is positive, and the detection result of transferrin is negative.
If the transferrin monoclonal antibody detection line 3131, the hemoglobin monoclonal antibody detection line 3132 and the goat anti-mouse polyclonal antibody control line 3133 are all colored, it is indicated that the content of transferrin and hemoglobin in the sample to be detected exceeds a predetermined value, and the detection results of transferrin and hemoglobin are positive.
If the transferrin monoclonal antibody detection line 3131, the hemoglobin monoclonal antibody detection line 3132 and the goat anti-mouse polyclonal antibody control line 3133 are not colored, this detection failure (possibly damage of the combined detection device or error in detection operation, etc.) is indicated.
It will be appreciated that a positive test for either of transferrin and hemoglobin indicates bleeding from the digestive tract.
As for the test paper strip 32 for detecting the helicobacter pylori antigen, if only the goat anti-mouse polyclonal antibody and the streptavidin control line 3232 are developed, the content of the helicobacter pylori antigen in the sample to be detected does not exceed a preset value, and the detection results of the helicobacter pylori antigen are all negative.
If the helicobacter pylori antigen monoclonal antibody detection line 3231 and the goat anti-mouse polyclonal antibody and the streptavidin control line 3232 are developed, the content of the helicobacter pylori antigen in the sample to be detected exceeds a preset value, and the detection result of the helicobacter pylori antigen is positive.
If the helicobacter pylori antigen monoclonal antibody detection line 3231 and the goat anti-mouse polyclonal antibody and the streptavidin control line 3232 do not develop color, the detection fails (the combined detection device is damaged or the detection operation is wrong, and the like).
(second embodiment)
A second embodiment of the combined detection device for transferrin, hemoglobin and helicobacter pylori antigen according to the present invention is described in detail below with reference to FIG. 6.
In this embodiment, as shown in fig. 6, the combined detection device for transferrin, hemoglobin and helicobacter pylori antigen according to the present invention comprises an upper casing 1, a lower casing 2 and a detection test strip assembly 4.
The structure of the upper case 1 and the lower case 2 in the present embodiment is substantially the same as that in the first embodiment. That is, the upper case 1 includes an upper case body 11, a sample input hole 12, and an observation window 13. The lower case 2 includes a lower case body 21 and a mounting groove 22.
The difference is mainly that the number of the sample input holes 12, the observation windows 13, and the mounting grooves 22 in the present embodiment is three.
In the present embodiment, as shown in fig. 6, the test strip assembly 4 includes a transferrin test strip 41, a hemoglobin test strip 42, and a helicobacter pylori antigen test strip 43. Wherein, the transferrin test strip 41, the hemoglobin test strip 42 and the helicobacter pylori antigen test strip 43 are respectively arranged in the corresponding mounting grooves 22.
In this embodiment, the transferrin test strip 41 and the hemoglobin test strip 42 are similar to the transferrin and hemoglobin test strip 31 of the first embodiment. That is, the transferrin test strip 41 includes a third bottom plate, a third absorbent plate, a third nitrocellulose membrane, a third sample liquid absorbent layer, and a third color particle storage pad, and the hemoglobin test strip 42 includes a fourth bottom plate, a fourth absorbent plate, a fourth nitrocellulose membrane, a fourth sample liquid absorbent layer, and a fourth color particle storage pad, and the connection structure between the respective portions is the same as that in the first embodiment.
The difference is mainly that the third color particle storage pad in the transferrin detection test strip 41 in this embodiment contains only transferrin monoclonal antibody, and does not contain hemoglobin monoclonal antibody. The third nitrocellulose membrane in the transferrin detection test strip 41 is only provided with a transferrin monoclonal antibody detection line and a goat anti mouse polyclonal antibody control line which are separated from each other, and is not provided with a hemoglobin monoclonal antibody detection line.
The fourth color particle storage pad of the hemoglobin detection test strip 42 in this embodiment contains only hemoglobin monoclonal antibody and does not contain transferrin monoclonal antibody. The fourth nitrocellulose membrane in the hemoglobin detection test strip 42 is only provided with a hemoglobin monoclonal antibody detection line and a goat anti-mouse polyclonal antibody control line which are separated from each other, and is not provided with a transferrin monoclonal antibody detection line.
In this embodiment, the test strip 43 for helicobacter pylori antigen detection has the same structure as the test strip 32 for helicobacter pylori antigen detection in the first embodiment, and will not be described herein.
In the present embodiment, similarly to the first embodiment, the color particles may each be colloidal gold particles, and accordingly, the third color particle storage pad and the fourth color particle storage pad may each be gold-labeled pads.
In this embodiment, the working principle of the combined detection device for transferrin, hemoglobin and helicobacter pylori antigen according to the present invention is basically the same as that of the first embodiment. That is, when only the control line develops color, the detection result is negative, when the detection line and the control line develop color simultaneously, the detection result is positive, and when neither the detection line nor the control line develops color, the detection failure is indicated.
Through adopting above-mentioned technical scheme, according to the utility model discloses a combined detection device of transferrin, hemoglobin and helicobacter pylori antigen has following advantage at least:
(1) the utility model discloses an among the combined detection device of transferrin, hemoglobin and helicobacter pylori antigen, can detect transferrin, hemoglobin and helicobacter pylori antigen simultaneously through setting up the detection test paper strip subassembly that can detect transferrin, hemoglobin and helicobacter pylori antigen simultaneously.
(2) The utility model discloses an among the combined detection device of transferrin, hemoglobin and helicobacter pylori antigen, through setting up the observation window, can conveniently learn the testing result.
The above embodiments of the present invention have been described in detail, but it should be added that:
(1) although the sample input hole is described as a circular hole in the above embodiment, the present invention is not limited thereto, and the shape of the sample input hole may be a polygon or other shapes.
(2) Although the shape of the observation window is described as a rectangle in the above embodiment, the present invention is not limited thereto, and the shape of the observation window may be a circle, an ellipse, or another polygon.
(3) Although it is described in the above embodiments that the number of the sample input holes is two or three, the present invention is not limited thereto, and two or three sample input holes may be directly configured as one larger hole as long as samples can be added to different test strips.
(4) Although the number of the observation windows is two or three in the above embodiment, the present invention is not limited thereto, and two or three observation windows may be directly configured as one large observation window, and only the detection result of each detection test strip may be observed.
(5) Although it is described in the above embodiment that the color particles are colloidal gold particles, the present invention is not limited thereto, and the color particles may be colloidal silver particles, colloidal iron particles, magnetic particles, dye particles, latex particles, or fluorescent particles.
(6) Although it is described in the above embodiment that the upper case and the lower case are both rectangular, the present invention is not limited thereto, and the upper case and the lower case may be circular, oval, or other shapes.
Claims (8)
1. A combined detection device for transferrin, hemoglobin and helicobacter pylori antigens is characterized by comprising an upper shell (1), a lower shell (2) and a detection test strip component,
the detection test strip component is arranged between the upper shell (1) and the lower shell (2) and is used for simultaneously detecting the transferrin, the hemoglobin and the helicobacter pylori antigen,
the test strip component comprises a helicobacter pylori antigen test strip used for detecting the helicobacter pylori antigen,
the helicobacter pylori antigen detection test strip comprises a second bottom plate (321), a second water absorption plate (322), a second nitrocellulose membrane (323), a second sample liquid absorption layer (324) and a second color particle storage pad (325) containing a helicobacter pylori antigen monoclonal antibody and a biotin-bovine serum albumin conjugate,
the second nitrocellulose membrane (323) is loaded with a helicobacter pylori antigen monoclonal antibody detection line (3231), a goat anti-mouse polyclonal antibody and a streptavidin control line (3232) which are separated from each other.
2. The combined test device of claim 1, wherein the test strip assembly further comprises a transferrin and hemoglobin combined test strip (31), the transferrin and hemoglobin combined test strip (31) being configured to detect the transferrin and the hemoglobin, or
The test strip assembly further comprises a transferrin test strip (41) and a hemoglobin test strip (42), wherein the transferrin test strip (41) is used for detecting transferrin, and the hemoglobin test strip (42) is used for detecting hemoglobin.
3. The combined detection device according to claim 2, wherein the transferrin and hemoglobin combined detection strip (31) comprises a first bottom sheet (311), a first water absorbent sheet (312), a first nitrocellulose membrane (313), a first sample liquid absorbent layer (314), and a first color particle storage pad (315) containing transferrin mab and hemoglobin mab,
the first nitrocellulose membrane (313) is loaded with a transferrin monoclonal antibody detection line (3131), a hemoglobin monoclonal antibody detection line (3132) and a goat anti-mouse polyclonal antibody control line (3133) which are separated from each other.
4. The joint detection device of claim 2, wherein the transferrin detection strip (41) comprises a third base plate, a third absorbent plate, a third nitrocellulose membrane, a third sample liquid absorbent layer, and a third color particle storage pad comprising transferrin monoclonal antibody,
and the third nitrocellulose membrane is loaded with a transferrin monoclonal antibody detection line and a goat anti-mouse polyclonal antibody control line which are separated from each other.
5. The combination detection device of claim 2, wherein the hemoglobin test strip (42) comprises a fourth base plate, a fourth absorbent plate, a fourth nitrocellulose membrane, a fourth sample absorbent layer, and a fourth color particle storage pad containing hemoglobin monoclonal antibody,
and the fourth nitrocellulose membrane is loaded with a hemoglobin monoclonal antibody detection line and a goat anti-mouse polyclonal antibody control line which are separated from each other.
6. Joint detection device according to claim 1, characterized in that the upper housing (1) is provided with a sample input aperture (12) and a viewing window (13),
the sample input hole (12) is used for the sample to be detected to pass through for loading,
the observation window (13) is arranged opposite to the detection test strip component in the thickness direction of the combined detection device and is used for observing the detection result of the detection test strip component.
7. The joint detection device according to claim 1, wherein the lower housing (2) is provided with a mounting groove (22), and the test strip assembly is disposed in the mounting groove (22).
8. Joint detection device according to claim 7, characterized in that the mounting groove (22) is a rectangular groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921038888.2U CN211043408U (en) | 2019-07-04 | 2019-07-04 | Combined detection device for transferrin, hemoglobin and helicobacter pylori antigen |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921038888.2U CN211043408U (en) | 2019-07-04 | 2019-07-04 | Combined detection device for transferrin, hemoglobin and helicobacter pylori antigen |
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| CN211043408U true CN211043408U (en) | 2020-07-17 |
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| CN201921038888.2U Active CN211043408U (en) | 2019-07-04 | 2019-07-04 | Combined detection device for transferrin, hemoglobin and helicobacter pylori antigen |
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