CN211014313U

CN211014313U - Brucella antibody detection box

Info

Publication number: CN211014313U
Application number: CN201921885974.7U
Authority: CN
Inventors: 奇丽霞
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-10-29
Filing date: 2019-10-29
Publication date: 2020-07-14
Anticipated expiration: 2029-10-29

Abstract

The utility model provides a brucellosis antibody detection box, which puts diluents with different volumes into a diluent bottle in advance, and drips the sample into the diluent to directly finish dilution without allocation, thereby greatly simplifying the detection process and improving the detection accuracy; the diluent bottle can directly interact with the detection card, and releases the diluted sample to the detection assembly, so that the time for exposing the diluted sample is short, and the detection process is safer.

Description

Brucella antibody detection box

Technical Field

The utility model relates to a detect technical field, especially relate to a brucellosis antibody detection box.

Background

Brucella is a gram-negative bacillus pumilus, and animals such as cattle, sheep, pigs and the like are most susceptible to infectious abortion of dams. Humans can be infected by exposure to bacteria-bearing animals or by exposure to food-ill animals and their dairy products. The method has already prevailed in partial areas of China and is basically controlled at present. The Brucella can be divided into 6 species of Brucella ovis, bovine, porcine, murine ovis and canine Brucella, and 20 biotypes. The three Brucella strains of cattle, pigs and cattle are popular in China, and the Brucella disease of sheep is the most common.

The brucella bacteria firstly infect domestic animals, and people can be infected by contacting with the domestic animals, taking polluted milk and meat, inhaling dust containing bacteria or bacteria to enter an eye binding membrane and other ways.

Patent No. CN201720027675.4 discloses a Brucella antibody detection kit, which is prepared by diluting a sample with a diluent and dripping the diluted sample on a detection card by a dropper, wherein the concentration of different diluted samples is different, which causes the problems of complex detection and inaccurate detection, and the sample is exposed for a long time, which is easy to cause infection.

Therefore, a brucella antibody detection kit is needed to solve the problems of complex, inaccurate and unsafe detection process in the existing brucella antibody detection.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a brucellosis antibody detection box to solve the above-mentioned complicated and inaccurate, unsafe problem of testing process among the current brucellosis antibody detection of portion.

In order to achieve the above object, the utility model provides a following scheme:

the utility model provides a brucellosis antibody detection box, comprises a box body, the box body is buckled and is equipped with the lid, be equipped with several sample standing grooves and diluent standing groove in the box body, sample bottle is placed in the sample standing groove and is used for holding the sample, diluent bottle is placed in the diluent standing groove, and the different diluent of volume is used for the dilution of different concentrations of sample to be filled in each diluent bottle, the diluent bottle includes upper portion open-ended body and can dismantle the bottle lid of connecting at the opening part, the top of bottle lid is opened has the aperture, and the aperture is sealed by the film;

a detection card placing groove is further formed in the box body, a detection card is placed in the detection card placing groove and comprises an upper shell and a lower shell which are buckled up and down, a sample adding hole is formed in the side face, connected with the upper shell and the lower shell, of the upper shell, the sample adding hole is matched with the bottle cap, a sharp prick is arranged in the sample adding hole and used for piercing a thin film at the small hole, an observation window is formed in the upper shell, and a detection assembly is clamped between the upper shell and the lower shell and used for detecting a diluted sample;

still be equipped with the burette standing groove in the box body, be equipped with the burette in the burette standing groove and be used for dripping of sample and get.

Preferably, the diluent in the diluent bottle is phosphate buffer, and the volumes of the diluent in the diluent bottle are respectively 12 drops, 15 drops, 18 drops, 21 drops, 24 drops, 27 drops and 30 drops by taking 1 drop of the dropper as a unit.

Preferably, the bottle body is soft plastics material, the bottle lid with bottle body threaded connection, the bottle lid is big end up's round platform form down for the diameter, the aperture is located the round platform top.

Preferably, the sample adding hole is a tapered hole matched with the bottle cap, the spike is fixed at the innermost part of the sample adding hole and connected with the lower shell, and the tip of the spike is outward.

Preferably, a transparent magnifying lens is arranged at the observation window.

Preferably, the detecting component includes the layer board, the layer board size with the epitheca with inferior valve inside dimension suits, be equipped with application of sample pad, nitrocellulose membrane and the paper that absorbs water that is equipped with in proper order on the layer board, application of sample pad with nitrocellulose membrane indirect connection, nitrocellulose membrane with paper direct connection absorbs water, application of sample pad accept in the application of sample hole, application of sample pad with nitrocellulose membrane top is hugged closely has the colloidal gold pad, nitrocellulose membrane is located under the observation window, and is equipped with test wire and control line on it in proper order.

Preferably, the sample adding pad adopts a glass fiber membrane coated with Brucella antigen.

The utility model discloses following beneficial technological effect has been gained for prior art:

the utility model provides a brucellosis antibody detection box, put into the diluent of different volumes in advance in the diluent bottle, drip the sample into the diluent and can directly accomplish the dilution, need not allotment, simplified the detection procedure greatly, also improved the precision of detection; the diluent bottle can directly interact with the detection card, and releases the diluted sample to the detection assembly, so that the time for exposing the diluted sample is short, and the detection process is safer.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive labor.

FIG. 1 is a top view of a portion of a box body and a box cover of a Brucella antibody detection box provided by the present invention;

FIG. 2 is a schematic view of a part of the structure of a diluent bottle of the Brucella antibody detection kit provided by the present invention;

FIG. 3 is a longitudinal sectional view of a portion of a card for detecting a Brucella antibody detecting cassette according to the present invention;

in the figure: 1: a box body and 11: sample placement groove, 12: diluent holding tank, 13: detection card placement slot, 14: burette standing groove, 2: a box cover and 3: sample bottle, 4: diluent bottle, 41: body, 42: bottle cap, 43: small hole, 5: detection card, 51: upper shell, 511: observation window, 52: lower shell, 53: sample addition hole, 54: spine, 55: detection assembly, 551: supporting plate, 552: sample addition pad, 553: nitrocellulose membrane, 554: absorbent paper, 555: colloidal gold pad, 6: a dropper.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

The utility model aims at providing a brucellosis antibody detection box through setting up matched with diluent bottle and detection card to solve the complicated and inaccurate, unsafe problem of testing process among the current brucellosis antibody detection.

In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description.

Example 1:

the embodiment provides a brucella antibody detection box, as shown in fig. 1, 2 and 3, which comprises a box body 1, wherein a box cover 2 is buckled on the box body 1, a plurality of sample placing grooves 11 and diluent placing grooves 12 are arranged in the box body 1, sample bottles 3 are placed in the sample placing grooves 11 and used for containing samples, diluent bottles 4 are placed in the diluent placing grooves 12, diluent bottles 4 with different volumes are contained in each diluent bottle 4 and used for diluting the samples with different concentrations, each diluent bottle 4 comprises a bottle body 41 with an upper opening and a bottle cover 42 detachably connected to the opening, a small hole 43 is formed in the top end of each bottle cover, and the small hole 43 is sealed by a thin film; a detection card placing groove 13 is further formed in the box body 1, a detection card 5 is placed in the detection card placing groove 13, the detection card 5 comprises an upper shell 51 and a lower shell 52 which are buckled up and down, a sample adding hole 53 is formed in the side face, connected with the upper shell 51 and the lower shell 52, of the detection card, the sample adding hole 53 is matched with the bottle cap 42, a sharp prick 54 is arranged in the sample adding hole 53 and used for pricking a thin film at the position of the small hole 43, the upper shell 51 is provided with an observation window 511, and a detection assembly 55 is clamped between the upper shell 51 and the lower shell 52 and used for detecting a diluted; a burette placing groove 14 is further arranged in the box body 1, and a burette 6 is arranged in the burette placing groove 14 and used for dripping and taking a sample.

Specifically, the diluent in the diluent bottle 4 is phosphate buffer solution, the main components of which are disodium hydrogen phosphate, potassium dihydrogen phosphate, sodium chloride, potassium chloride and sodium azide, and the sample may be serum, whole blood, milk sample and the like, and the adopted dilution ratios are different, so the volumes of the diluent in the diluent bottle 4 are 12 drops, 15 drops, 18 drops, 21 drops, 24 drops, 27 drops and 30 drops respectively by taking 1 drop of the dropper 6 as a unit, and the dilution can be completed by only dropping 1 drop of the sample in the corresponding diluent without blending, thereby simplifying the detection steps.

Further, the bottle body 41 is made of soft plastic materials, so that the sample can be conveniently extruded and released to be diluted, the bottle cap 42 is in threaded connection with the bottle body 41, the bottle cap 42 is in a circular truncated cone shape with a large diameter at the bottom and a small diameter at the top, and the small hole 43 is positioned at the top of the circular truncated cone.

Furthermore, the sampling hole 53 is a tapered hole matched with the bottle cap 42, the spike 54 is fixed at the innermost part of the sampling hole 53 and connected with the lower shell 52, the tip of the spike 54 is outward, the bottle cap 42 is inserted into the sampling hole 53 to pierce the thin film at the small hole 43, and the diluted sample can be released by pressing the bottle body 41.

Furthermore, a transparent magnifying lens is disposed at the observation window 511 for facilitating observation.

Further, the detecting component 55 includes a support plate 551, the size of the support plate 551 is adapted to the internal size of the upper shell 51 and the lower shell 52, the support plate 551 is provided with a sample adding pad 552, a nitrocellulose membrane 553 and a water absorbing paper 554 which are sequentially arranged, the sample adding pad 552 adopts a glass fiber membrane coated with brucella antigen, the sample adding pad 552 and the nitrocellulose membrane 553 are not directly connected, the nitrocellulose membrane 553 is directly connected with the water absorbing paper 554, the sample adding pad 552 is received in the sample adding hole 53, a colloidal gold pad 555 is closely attached above the sample adding pad 552 and the nitrocellulose membrane 553, the nitrocellulose membrane 553 is positioned right below the observation window 511, and a testing line and a control line are sequentially arranged thereon, after the diluted sample enters the detection assembly 55, the diluted sample passes through the sample addition pad 552, the colloidal gold pad 555 and the nitrocellulose membrane 553 in sequence, and finally the result is displayed on the nitrocellulose membrane 553, and the excessive diluted sample is absorbed by the absorbent paper 554.

The utility model provides a pair of brucellosis antibody detection box, when using, at first gather in sample to the sample bottle 3, select the diluent bottle 4 of suitable dilution proportion according to the kind of sample, open bottle lid 42, use burette 6 to get a drop of sample in the diluent of diluent bottle 4, it shakes evenly to screw up screw cap 4, aim at bottle lid 4 with the appearance hole 53, pressurize gently in the body 41 can release the sample after diluting to detecting element 55, can observe the testing result through observation window 511 after a few minutes, the diluent bottle 4 after the use directly abandons with detecting element 55.

The utility model discloses the principle and the implementation mode of the utility model are explained by applying the concrete examples, and the explanation of the above examples is only used for helping to understand the method and the core idea of the utility model; meanwhile, for the general technical personnel in the field, according to the idea of the present invention, there are changes in the concrete implementation and the application scope. In summary, the content of the present description should not be construed as a limitation of the present invention.

Claims

1. A brucella antibody detection kit, which is characterized in that: the box comprises a box body, wherein a box cover is buckled on the box body, a plurality of sample placing grooves and diluent placing grooves are arranged in the box body, sample bottles are placed in the sample placing grooves and used for containing samples, diluent bottles are placed in the diluent placing grooves, diluent with different volumes is placed in each diluent bottle and used for diluting the samples with different concentrations, each diluent bottle comprises a bottle body with an upper opening and a bottle cover detachably connected to the opening, and the top end of each bottle cover is provided with a small hole which is sealed by a thin film;

2. The Brucella antibody test kit of claim 1, wherein: the diluent in the diluent bottle is phosphate buffer solution, and the volumes of the diluent in the diluent bottle are respectively 12 drops, 15 drops, 18 drops, 21 drops, 24 drops, 27 drops and 30 drops by taking 1 drop of the dropper as a unit.

3. The Brucella antibody test kit of claim 2, wherein: the bottle body is made of soft plastic materials, the bottle cap is in threaded connection with the bottle body, the bottle cap is in a circular truncated cone shape with the diameter being large at the bottom and small at the top, and the small hole is located in the top of the circular truncated cone.

4. The Brucella antibody test kit of claim 3, wherein: the sample adding hole is a conical hole matched with the bottle cap, the sharp prick is fixed at the innermost part of the sample adding hole and connected with the lower shell, and the sharp end of the sharp prick faces outwards.

5. The Brucella antibody test kit of claim 4, wherein: and a transparent magnifying lens is arranged at the observation window.

6. The Brucella antibody test kit of claim 5, wherein: the detection assembly comprises a supporting plate, the size of the supporting plate is matched with the size of the upper shell and the size of the inner part of the lower shell, a sample adding pad, a nitrocellulose membrane and water absorbing paper are arranged on the supporting plate in sequence, the sample adding pad is not directly connected with the nitrocellulose membrane, the nitrocellulose membrane is directly connected with the water absorbing paper, the sample adding pad is connected with a sample adding hole, the sample adding pad is tightly attached to the nitrocellulose membrane, a colloidal gold pad is attached to the top of the nitrocellulose membrane, the nitrocellulose membrane is located under the observation window, and a test line and a control line are sequentially arranged on the nitrocellulose membrane.

7. The Brucella antibody test kit of claim 6, wherein: the sample adding pad adopts a glass fiber membrane coated with Brucella antigen.