CN211005374U

CN211005374U - Plasmid DNA extraction equipment

Info

Publication number: CN211005374U
Application number: CN201921619250.8U
Authority: CN
Inventors: 赵锐
Original assignee: Beiken Biotechnology Dalian Co Ltd
Current assignee: Beiken Biotechnology Dalian Co Ltd
Priority date: 2019-09-26
Filing date: 2019-09-26
Publication date: 2020-07-14
Anticipated expiration: 2029-09-26

Abstract

The utility model relates to a DNA draws technical field, and discloses a plasmid DNA draws equipment, including the storage bottle, the middle part fixed mounting of storage bottle inner chamber has first rubber seal cover and second rubber seal cover, second rubber seal cover is located the below of first rubber seal cover, the inner wall swing joint of first rubber seal cover and second rubber seal cover has the extraction tube, the storage storehouse has been seted up to the bottom of storage bottle, the bottom swing joint of storage bottle has the external member. The utility model discloses an utilize the organizational structure of first rubber seal cover, second rubber seal cover, the device can seal the DNA of drawing, make its first rubber seal cover and second rubber seal cover play the guard action to the DNA of inside storage, effectually prevented DNA and air contact, and then lead to its inside molecule to react with the molecule in the air, and then influence the effect of its experiment, the device is effectual has improved the quality that DNA drawed, and then the effectual precision that improves the experiment.

Description

Plasmid DNA extraction equipment

Technical Field

The utility model relates to a DNA draws technical field, specifically is a plasmid DNA draws equipment.

Background

Deoxyribonucleic acid is one of four kinds of biological macromolecules contained in biological cells, DNA carries genetic information necessary for synthesizing RNA and protein and is a biological macromolecule indispensable for development and normal operation of organisms, and DNA is a macromolecular polymer consisting of deoxynucleotides, which are composed of bases, deoxyribose and phosphate, wherein the bases are 4: adenine, guanine, thymine and cytosine, in the DNA molecular structure, two polydeoxyribonucleotide chains are coiled around a common central shaft to form a double-helix structure, a deoxyribose-phosphate chain is arranged outside the helix structure, a base faces to the inside, the two polydeoxyribonucleotide chains are in reverse complementation, and are connected through base pairing formed by hydrogen bonds among the bases to form a quite stable combination.

The existing DNA can be contacted with air in the extraction process, the quality of the DNA can be changed when the DNA is contacted with the air, or the molecular contact in the air can lead the internal molecular structure of the DNA to be changed, so that the experimental quality of the extracted DNA is not high, most of the existing DNA is directly poured into an experimental vessel after extraction, the DNA is contacted with the air in the mode, the experimental effect of the DNA is influenced, and therefore the plasmid DNA extraction equipment is provided.

SUMMERY OF THE UTILITY MODEL

The not enough to prior art, the utility model provides a plasmid DNA draws equipment, possess effectual avoiding its and air contact after drawing, the quality of its extraction has been improved, can dock its bottom and household utensils when using, prevent to influence the advantage of experiment effect with air contact, the in-process of having solved current DNA and drawing can contact with the air, can make the quality of DNA change when contacting with the air, or can lead to its inside molecular structure to change with the molecular contact in the air, and then the DNA experiment quality after leading to drawing is not high, and current DNA is most directly topples over it after drawing and put into the laboratory utensils, this kind of mode can lead to its DNA and air contact, influence the problem of its experiment effect.

The utility model provides a following technical scheme: the utility model provides a plasmid DNA draws equipment, includes the storage bottle, the middle part fixed mounting of storage bottle inner chamber has first rubber seal cover and second rubber seal cover, second rubber seal cover is located the below of first rubber seal cover, the inner wall swing joint of first rubber seal cover and second rubber seal cover has the extraction tube, the storage compartment has been seted up to the bottom of storage bottle, the bottom swing joint of storage bottle has the external member, the outer wall fixedly connected with fixed block of external member, the bottom fixed mounting of external member inner wall has the sealing plug, the through-hole has been seted up to the bottom of storage bottle, the sealing plug is with the through-hole looks adaptation of storage bottle bottom.

Preferably, the sizes of the first rubber sealing sleeve and the second rubber sealing sleeve are equal, and the outer walls of the first rubber sealing sleeve and the second rubber sealing sleeve are fixedly connected with the inner wall of the storage bottle.

Preferably, the length value of the storage bottle is greater than the length value of the extraction tube, the diameter value of the storage bottle is greater than the diameter value of the extraction tube, and the extraction tube is positioned in the middle of the inner cavity of the storage bottle.

Preferably, the fixed block is movably connected with the outer wall of the bottom of the storage bottle, the inner wall of the fixed block is provided with a latch, the outer wall of the bottom of the storage bottle is provided with a clamping groove, and the latch clamping grooves are meshed with each other.

Preferably, the storage bottle and the extraction tube are both made of glass tubes.

Compared with the prior art, the utility model discloses possess following beneficial effect:

1. this plasmid DNA draws equipment, through utilizing first rubber seal cover, the organizational structure of second rubber seal cover, the device can seal the DNA that draws, make its first rubber seal cover and second rubber seal cover play the guard action to the DNA of inside storage, the effectual DNA and air contact that has prevented, and then lead to its inside molecule to react with the molecule in the air, and then influence the effect of its experiment, the device is effectual has improved the quality that DNA drawed, and then the effectual precision that has improved the experiment.

2. This plasmid DNA draws equipment, through the organizational structure who utilizes fixed block, external member and sealing plug, the device can seal up the DNA after extracting and keep, and the device has effectually prevented that DNA and air contact from leading to the molecule in its inside molecule and the air to react, and when using, can dock its bottom and household utensils, has improved the precision of experiment.

Drawings

FIG. 1 is a schematic view of the interior of the structure of the present invention;

fig. 2 is a schematic structural diagram of the present invention.

In the figure: 1. a storage bottle; 2. an extraction tube; 3. a first rubber sealing sleeve; 4. a second rubber sealing sleeve; 5. a storage bin; 6. a fixed block; 7. a kit; 8. and (4) sealing the plug.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-2, a plasmid DNA extraction device, including a storage bottle 1, a first rubber sealing sleeve 3 and a second rubber sealing sleeve 4 are fixedly installed in the middle of the inner cavity of the storage bottle 1, the device can seal the extracted DNA, so that the first rubber sealing sleeve 3 and the second rubber sealing sleeve 4 can protect the DNA stored inside, effectively prevent the DNA from contacting with air, further cause the molecules inside to react with the molecules in the air, further affect the experimental effect, the device effectively improves the DNA extraction quality, further effectively improves the experimental accuracy, the first rubber sealing sleeve 3 and the second rubber sealing sleeve 4 have the same size, the outer walls of the first rubber sealing sleeve 3 and the second rubber sealing sleeve 4 are both fixedly connected with the inner wall of the storage bottle 1, the second rubber sealing sleeve 4 is located below the first rubber sealing sleeve 3, the inner walls of the first rubber sealing sleeve 3 and the second rubber sealing sleeve 4 are movably connected with an extraction tube 2, the device is used for pouring DNA into the storage bottle 1 through a pipeline thereof to prevent the contact of other air, the storage bottle 1 and the extraction tube 2 are both prepared from glass tubes, the length value of the storage bottle 1 is larger than that of the extraction tube 2, the diameter value of the storage bottle 1 is larger than that of the extraction tube 2, the extraction tube 2 is positioned in the middle of the inner cavity of the storage bottle 1, the bottom of the storage bottle 1 is provided with a storage bin 5, the bottom of the storage bottle 1 is movably connected with a sleeve member 7, the outer wall of the sleeve member 7 is fixedly connected with a fixed block 6, the device can seal and store the extracted DNA, the device effectively prevents the molecules inside the DNA from reacting with the molecules in the air due to the contact of the DNA and the air, and can butt the bottom of the device with a vessel when in use, thereby improving the accuracy, the outer wall swing joint of fixed block 6 and 1 bottom of storage bottle, the latch has been seted up to the inner wall of fixed block 6, the draw-in groove has been seted up to the outer wall of 1 bottom of storage bottle, and the latch draw-in groove meshes mutually, the bottom fixed mounting of 7 inner walls of external member has sealing plug 8, the through-hole has been seted up to the bottom of storage bottle 1, it is convenient for take it when using to set up the through-hole, and prevent to contact with the air, lead to its molecular fission, the through-hole looks adaptation of sealing plug 8 and 1 bottom of storage bottle.

The theory of operation stretches into the inside of storage bottle 1 with extraction tube 2 to run through first rubber seal cover 3 and second rubber seal cover 4 and extend to the inside of storage bottle 1 with it, carry the inner chamber to storage bottle 1 with DNA through the mouth of pipe of extraction tube 2, can.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A plasmid DNA extraction apparatus comprising a storage bottle (1), characterized in that: the utility model discloses a storage bottle, including storage bottle (1), inner chamber, inner wall swing joint have extraction tube (2), the middle part fixed mounting of storage bottle (1) inner chamber has first rubber seal cover (3) and second rubber seal cover (4), second rubber seal cover (4) are located the below of first rubber seal cover (3), the inner wall swing joint of first rubber seal cover (3) and second rubber seal cover (4) has extraction tube (2), storage bin (5) have been seted up to the bottom of storage bottle (1), the bottom swing joint of storage bottle (1) has external member (7), the outer wall fixedly connected with fixed block (6) of external member (7), the bottom fixed mounting of external member (7) inner wall has sealing plug (8), the through-hole has been seted up to the bottom of storage bottle (1), the through-hole looks adaptation of sealing plug (8) and storage bottle (1.

2. The plasmid DNA extraction apparatus according to claim 1, characterized in that: the size of the first rubber sealing sleeve (3) is equal to that of the second rubber sealing sleeve (4), and the outer walls of the first rubber sealing sleeve (3) and the second rubber sealing sleeve (4) are fixedly connected with the inner wall of the storage bottle (1).

3. The plasmid DNA extraction apparatus according to claim 1, characterized in that: the length value of storage bottle (1) is greater than the length value of extraction tube (2), the diameter value of storage bottle (1) is greater than the diameter value of extraction tube (2), extraction tube (2) are located the middle part of storage bottle (1) inner chamber.

4. The plasmid DNA extraction apparatus according to claim 1, characterized in that: the outer wall swing joint of fixed block (6) and storage bottle (1) bottom, the latch has been seted up to the inner wall of fixed block (6), the draw-in groove has been seted up to the outer wall of storage bottle (1) bottom, and the latch draw-in groove meshes mutually.

5. The plasmid DNA extraction apparatus according to claim 1, characterized in that: the storage bottle (1) and the extraction tube (2) are both made of glass tubes.