CN211005326U

CN211005326U - Marine animal DNA extractor

Info

Publication number: CN211005326U
Application number: CN201921618142.9U
Authority: CN
Inventors: 赵锐
Original assignee: Beiken Biotechnology Dalian Co Ltd
Current assignee: Beiken Biotechnology Dalian Co Ltd
Priority date: 2019-09-26
Filing date: 2019-09-26
Publication date: 2020-07-14
Anticipated expiration: 2029-09-26

Abstract

The utility model relates to a biological detection technical field just discloses a marine animal DNA extractor, including the device support, the bottom fixed mounting of device support has places the case, the bottom fixed mounting who places the incasement chamber has first servo motor, first servo motor's output runs through the top fixed mounting who places the case and extend to place the case and has centrifugal carousel, centrifugal carousel's side threaded mounting has the lead screw, the one end of lead screw is located the inside one end movable mounting of centrifugal carousel and has the connecting plate, the other end fixedly connected with spring of connecting plate. This marine animal DNA extractor can be so that the inside of glass homogenizer carries out quick homogenate operation then can be quick through manual valve with the quick test tube on the inflow test tube clamping device of homogenate and then make the middle transport speed faster through the cooperation with link and fastening screw and intermediate junction box and connection pad, and the centre need not to delay time, and then guarantees the activity of DNA.

Description

Marine animal DNA extractor

Technical Field

The utility model relates to a biological detection technical field specifically is a marine animal DNA extractor.

Background

The marine life refers to living species in the sea, including marine animals, marine plants, microorganisms, viruses, etc., wherein the marine animals include invertebrates and vertebrates, the invertebrates include various snails and shellfishes, and the vertebrates include various fishes and large marine animals, such as red starfish, whale, shark, etc.

When extracting the DNA of marine organism, because some marine organisms go on fast rotting easily to appear when carrying out DNA extraction, and then make the activity of DNA decline and then make the effect of DNA extraction not good, need carry out a plurality of steps when carrying out DNA extraction, need use multiple apparatus moreover, the time of going on in the middle is longer, and is troublesome, and speed is slower, so need a DNA extractor that marine animal used to carry out the extraction of DNA.

SUMMERY OF THE UTILITY MODEL

The not enough to prior art, the utility model provides a marine animal DNA extractor possesses simple structure, and the speed of extraction is fast, can prevent that the marine animal from appearing rotting the active not enough advantage that leads to extracting, has solved the problem of mentioning in the above background art.

The utility model provides a following technical scheme: a marine animal DNA extractor comprises a device support, wherein a placing box is fixedly mounted at the bottom of the device support, a first servo motor is fixedly mounted at the bottom of an inner cavity of the placing box, the output end of the first servo motor penetrates through the placing box and extends to the upper part of the placing box, a centrifugal turntable is fixedly mounted at the upper part of the placing box, a lead screw is mounted on the side surface of the centrifugal turntable through threads, a connecting plate is movably mounted at one end of the lead screw, which is positioned inside the centrifugal turntable, a spring is fixedly connected to the other end of the connecting plate, a test tube clamping plate is fixedly connected to the other end of the spring, a test tube placing frame is fixedly mounted on the right side surface of the device support, a standby test tube is placed on the front surface of the test tube placing frame, a heating box is fixedly mounted on the inner side surface of the device support, an electromagnetic heating coil is, the bottom of the heating crucible inner cavity is fixedly provided with a waterproof ultrasonic oscillation device, the inner side of the heating crucible is fixedly provided with a test tube clamping device, the right side of the heating crucible inner cavity is fixedly provided with a temperature sensor, the top of the inner side of the device support is fixedly provided with a mounting seat, the inside of the mounting seat is fixedly provided with a second servo motor, the output end of the second servo motor penetrates through the mounting seat and extends to the lower part of the mounting seat and is fixedly connected with an intermediate coupling, the bottom of the mounting seat is movably provided with a connecting frame, the side surface of the mounting seat is in threaded connection with a fastening screw matched with the connecting frame, the bottom of the connecting frame is fixedly connected with a glass homogenizer, the inner side of the glass homogenizer is movably provided with a stirring device, the top of the stirring device is fixedly connected with a connecting disc, and the bottom of the glass homogenizer is fixedly communicated with a manual valve.

Preferably, the controller is installed on the front surface of the device support, and the controller is electrically connected with the temperature sensor and the electromagnetic heating coil.

Preferably, the number of the test tube clamping plates is two, and the two test tube clamping plates are symmetrically distributed by taking the central line of the centrifugal turntable as a symmetry axis.

Preferably, the test tube clamping position of the test tube clamping device is positioned right below the manual valve.

Preferably, the outer diameter value of the spring is 20mm, the inner diameter value is 15mm, and the steel grade value is 2 kg/cm.

Compared with the prior art, the utility model discloses possess following beneficial effect:

1. this marine animal DNA extractor can be so that the inside of glass homogenizer carries out quick homogenate operation then can be quick through manual valve with the quick test tube on the inflow test tube clamping device of homogenate and then make the middle transport speed faster through the cooperation with link and fastening screw and intermediate junction box and connection pad, and the centre need not to delay time, and then guarantees the activity of DNA.

2. This marine animal DNA extractor, the water bath temperature that can guarantee to heat the crucible through electromagnetic heating coil and temperature sensor's cooperation keeps stable and then guarantees the activity of DNA, makes reagent and tissue also carry out the rapid response through waterproof ultrasonic oscillation device when carrying out the water bath, improves the speed of drawing, makes the test tube splint can clip the test tube through centrifugal carousel and lead screw and spring and carries out the centrifugation to mixed liquid and then draw DNA.

Drawings

FIG. 1 is a sectional view of the present invention;

FIG. 2 is an enlarged view of the structure A of the present invention;

fig. 3 is an enlarged view of the structure B of the present invention.

In the figure: 1. a device holder; 2. a temperature sensor; 3. placing a test tube rack; 4. a standby test tube; 5. a mounting seat; 6. a second servo motor; 7. a glass homogenizer; 8. a manual valve; 9. heating the crucible; 10. A test tube holding device; 11. a waterproof ultrasonic oscillation device; 12. a heating box; 13. an electromagnetic heating coil; 14. a centrifugal turntable; 15. a first servo motor; 16. placing a box; 17. fastening screws; 18. a connecting frame; 19. a connecting disc; 20. a stirring device; 21. an intermediate coupling; 22. a screw rod; 23. a spring; 24. a test tube clamping plate; 25. a connecting plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-3, a marine animal DNA extractor comprises a device support 1, a placing box 16 is fixedly installed at the bottom of the device support 1, a first servo motor 15 is fixedly installed at the bottom of an inner cavity of the placing box 16, an output end of the first servo motor 15 penetrates through the placing box 16 and extends to the upper side of the placing box 16 and is fixedly installed with a centrifugal turntable 14, a lead screw 22 is installed on the side surface of the centrifugal turntable 14 in a threaded manner, a connecting plate 25 is movably installed at one end of the lead screw 22, which is located inside the centrifugal turntable 14, a spring 23 is fixedly connected to the other end of the connecting plate 25, the outer diameter value of the spring 23 is 20mm, the inner diameter value is 15mm, the steel degree value is 2kg/cm, test tube clamping plates 24 are fixedly connected to the other end of the spring 23, the number of the test tube clamping plates 24 is two, the two test tube clamping plates 24 are symmetrically distributed by taking the center line of the centrifugal turntable 14 as a symmetric axis Centrifuging and further extracting DNA, a test tube placing rack 3 is fixedly arranged on the right side surface of the device support 1, a standby test tube 4 is placed on the front surface of the test tube placing rack 3, a heating box 12 is fixedly arranged on the inner side surface of the device support 1, an electromagnetic heating coil 13 is fixedly arranged below the inner cavity of the heating box 12, a heating crucible 9 is fixedly arranged above the electromagnetic heating coil 13 in the heating box 12, a waterproof ultrasonic oscillation device 11 is fixedly arranged at the bottom of the inner cavity of the heating crucible 9, a test tube clamping device 10 is fixedly arranged on the inner side of the heating crucible 9, a test tube clamping position of the test tube clamping device 10 is positioned under a manual valve 8, a temperature sensor 2 is fixedly arranged on the right side of the inner cavity of the heating crucible 9, a controller is arranged on the front surface of the device support 1, and the controller is, the water bath temperature of the heating crucible 9 can be kept stable through the matching of the electromagnetic heating coil 13 and the temperature sensor 2, so that the activity of DNA is guaranteed, the reagent and tissues can also react rapidly through the waterproof ultrasonic oscillation device 11 while water bath is carried out, the extraction speed is improved, the top of the inner side of the device support 1 is fixedly provided with the mounting seat 5, the inner part of the mounting seat 5 is fixedly provided with the second servo motor 6, the output end of the second servo motor 6 penetrates through the mounting seat 5 and extends to the lower part of the mounting seat 5 and is fixedly connected with the middle coupler 21, the bottom of the mounting seat 5 is movably provided with the connecting frame 18, the side surface of the mounting seat 5 is in threaded connection with the fastening screw 17 matched with the connecting frame 18, the bottom of the connecting frame 18 is fixedly connected with the glass homogenizer 7, the inner side of the glass homogenizer 7 is movably provided with the stirring device 20, the top of the stirring device 20 is fixedly connected, the fixed intercommunication in bottom of glass homogenizer 7 has hand valve 8, can make the inside of glass homogenizer 7 carry out quick homogenate operation then through hand valve 8 can be quick with the quick test tube that flows into on test tube clamping device 10 of homogenate through the cooperation with link 18 and fastening screw 17 and intermediate junction 21 and connection pad 19 and then make the centre transfer speed faster, the centre need not to delay time, and then guarantees the activity of DNA.

The working principle is that firstly, 0.1g of fresh or frozen animal tissue blocks are taken, the fresh or frozen animal tissue blocks are cut into pieces as much as possible and placed in a glass homogenizer 7, a cell lysis buffer solution is added, then the glass homogenizer 7 is connected with a mounting seat 5 through a fastening screw 17 and a connecting frame 18, at the moment, an intermediate coupling 21 is connected with a connecting disc 19 and then drives a stirring device 20 to be connected through a second servo motor 6 so as to homogenize, when the tissue blocks are not found after homogenization, then a manual valve 8 is opened so that a mixed solution flows into a test tube, simultaneously protease is added, at the moment, an electromagnetic heating coil 13 heats a heating crucible 9 so as to carry out water bath on the mixed solution, a temperature sensor 2 detects the water bath temperature so as to ensure that the temperature is in a stable state, meanwhile, a waterproof ultrasonic oscillation device 11 carries out intermittent oscillation on the mixed solution, then, rotating the screw rod 22 to enable the force of the spring 23 to act on the test tube clamping plate 24 to clamp the test tube, driving the centrifugal turntable 14 to rotate by the first servo motor 15 to carry out centrifugation, pouring supernate into another test tube, adding isopropanol with 2 times of volume, turning upside down and mixing uniformly to enable filaments to be seen, picking out the filaments by a 100-pl gun head, airing, dissolving again by TE buffer solution, adding a mixed solution of phenol, chloroform and isoamyl alcohol with the same volume, shaking and mixing uniformly, then carrying out centrifugation, taking the upper layer solution into another tube, adding chloroform and isoamyl alcohol with the same volume, shaking and mixing uniformly, centrifuging, adding other reagents to carry out centrifugation, taking out precipitate, adding 200-pl TE to dissolve the precipitate again, and then storing at 4 ℃ or-20 ℃ for later use.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A marine animal DNA extractor, includes device support (1), its characterized in that: the device comprises a device support (1), a placing box (16) is fixedly installed at the bottom of the device support (1), a first servo motor (15) is fixedly installed at the bottom of an inner cavity of the placing box (16), an output end of the first servo motor (15) penetrates through the placing box (16) and extends to the upper portion of the placing box (16) to be fixedly installed with a centrifugal turntable (14), a lead screw (22) is installed on the side face of the centrifugal turntable (14) in a threaded mode, a connecting plate (25) is movably installed at one end, located inside the centrifugal turntable (14), of one end of the lead screw (22), a spring (23) is fixedly connected to the other end of the connecting plate (25), a test tube clamping plate (24) is fixedly connected to the other end of the spring (23), a test tube placing frame (3) is fixedly installed on the right side face of the device support (1, the device comprises a device support (1), a heating box (12) is fixedly mounted on the inner side of the device support (1), an electromagnetic heating coil (13) is fixedly mounted on the lower portion of an inner cavity of the heating box (12), a heating crucible (9) is fixedly mounted on the upper portion of the inner cavity of the heating box (12), a waterproof ultrasonic oscillation device (11) is fixedly mounted on the bottom of the inner cavity of the heating crucible (9), a test tube clamping device (10) is fixedly mounted on the inner side of the heating crucible (9), a temperature sensor (2) is fixedly mounted on the right side of the inner cavity of the heating crucible (9), a mounting seat (5) is fixedly mounted on the top of the inner side of the device support (1), a second servo motor (6) is fixedly mounted on the inner portion of the mounting seat (5), the output end of the second servo motor (6) penetrates through the mounting seat (5) and extends to the lower portion of the mounting seat (5, the utility model discloses a glass homogenizer, including mount pad (5), the bottom movable mounting of mount pad (5) has link (18), the side threaded connection of mount pad (5) have with link (18) matched with fastening screw (17), the bottom fixedly connected with glass homogenizer (7) of link (18), the inboard movable mounting of glass homogenizer (7) has agitating unit (20), the top fixedly connected with and intermediate junction case (21) matched with connection pad (19) of agitating unit (20), the fixed intercommunication in bottom of glass homogenizer (7) has hand valve (8).

2. The marine animal DNA extractor of claim 1, wherein: the front surface of the device support (1) is provided with a controller, and the controller is electrically connected with the temperature sensor (2) and the electromagnetic heating coil (13).

3. The marine animal DNA extractor of claim 1, wherein: the number of the test tube clamping plates (24) is two, and the two test tube clamping plates (24) are symmetrically distributed by taking the central line of the centrifugal turntable (14) as a symmetry axis.

4. The marine animal DNA extractor of claim 1, wherein: the test tube clamping position of the test tube clamping device (10) is positioned right below the manual valve (8).

5. The marine animal DNA extractor of claim 1, wherein: the outer diameter value of the spring (23) is 20mm, the inner diameter value is 15mm, and the steel degree value is 2 kg/cm.