CN210903494U - Animal trachea perfusion device - Google Patents

Abstract

The utility model discloses an animal trachea perfusion device, include: the catheter comprises a catheter and a light guide pipe which is nested and fixed with the catheter; one end of the liquid guide pipe is provided with an access port for connection, and the other end of the liquid guide pipe is provided with an opening for forming a drip liquid circulation channel; one end of the light guide pipe is connected with the illumination module through the light guide cone, and the other end of the light guide pipe, which is on the same side with the opening of the liquid guide pipe, is the front end, so that a light signal emitted by the illumination module is transmitted to the end face of the front end of the light guide pipe through the light guide pipe and is used for marking the opening position of the liquid guide pipe. The light guide pipe is used for transmitting the illumination light to replace the existing optical fiber for transmitting the illumination light, and the light guide pipe forms a hollow inner cavity and can be nested and fixed with the catheter, so that the problem of inaccurate positioning caused by the fact that the light guide device and the catheter cannot be relatively fixed and are necessarily retracted in the inserting process is fundamentally solved. The device has no wound on mice, is simple to operate, has high success rate, is economical and portable, can realize repeated exposure of toxicant or medicine for many times, and can accurately control the exposure dose of the toxicant or the medicine in vivo.

Description

Animal trachea perfusion device

Technical Field

The utility model belongs to the experimental apparatus field of biomedical, more specifically relates to an animal trachea perfusion device.

Background

Respiratory tract contamination is an important way for realizing exposure of granular toxicants (such as atmospheric particulates and productive dust) or medicines (such as inhalation medicines and carriers thereof) in animals through a respiratory tract, and is widely applied in the fields of toxicology, pharmacology, labor and environmental hygiene, clinical medicine and the like. The mouse is one of the most widely used and well-studied experimental animals, and has strict quality control standards, inbred lines, mutant lines, recombinant inbred lines and closed groups. The breeding of genetically engineered mice has also increased rapidly in recent years, and the application requirements of the genetically engineered mice in various experiments are on the rise. In the research process of granular toxicants or medicines, the respiratory tract infected mouse model is more and more widely applied.

At present, the establishment of a respiratory tract mouse contamination model mainly depends on an inhalation contamination device and a tracheal instillation contamination device. (1) Inhalation toxicant exposure device (whole body type inhalation exposure cabin or mouth nose type inhalation exposure device): the toxicant exposure was achieved by exposing the mice systemically or oronasally to air in which particulate matter was suspended. The device can simulate the normal breathing process of a mouse, is relatively simple to operate, can realize long-term repeated contamination, and has the defects of difficulty in accurately controlling the in vivo exposure dose, low utilization rate of poison or medicine, complex operation, high acquisition and maintenance cost and the like depending on a complex particulate generation and suspension system, so the utilization rate is lower. (2) The tracheal instillation contamination device: the contamination is realized by inserting a catheter in the trachea of the mouse and then injecting a particle suspension. The device has simple design, can accurately control the in vivo exposure dose, has the advantages of high utilization rate of the medicine or the poison, low price and the like, and is widely applied to animal experiments. However, the device generally has the defect of low success rate because the mouse trachea is thin (the inner diameter of the trachea is about 2mm under the resting state of an adult mouse), and the mouse is easy to die due to airway obstruction when being perfused with a particle suspension; in addition, the instillation site must be strictly controlled (about 1-2mm below the thyroid cartilage) or the cough reflex is induced to cause the release of the poison or drug, resulting in the failure of the molding. Aiming at the defects of the tracheal instillation contamination device, two solutions are mainly relied on in the past: one is to observe the oral direct-viewing trachea under a transmission lamp to determine the instillation position, but the success rate of the method cannot be obviously improved because the transmission lamp can not clearly position the thyroid cartilage of the mouse trachea; the other method is to use surgical operation to invasively expose the trachea and then insert a catheter through mouth to be contaminated, the method can accurately position the instillation position and improve the success rate of the experiment, but can not realize the long-term repeated exposure of the poison or the medicine, and the stress and inflammatory reaction caused by the surgical operation seriously influence the evaluation of the experiment effect.

Chinese patent document CN109011070 provides a visual mouse trachea cannula device, which comprises a power supply, a wire, a lamp, an optical fiber and a trachea catheter, wherein the power supply is connected with the lamp through the wire, one end of the optical fiber is inserted into the trachea catheter, the other end of the optical fiber is attached to the edge of the lamp, the optical fiber can provide illumination on the one hand, the hardness of the trachea catheter can be improved on the one hand, and the cannula is convenient. However, in this device, since a channel for loading a drug needs to be formed in the catheter and the optical fiber is nested, the catheter and the optical fiber cannot be fixed relatively but are in a free state, and the optical fiber is almost inevitably retracted during the process of tracheal intubation, so that the catheter is not positioned accurately, and the experiment fails. In addition, the front end of the optical fiber directly emits light to serve as a positioning signal in the design, and a point light source is formed at the front end to emit light forward to interfere positioning.

SUMMERY OF THE UTILITY MODEL

To the above defect or the improvement demand of prior art, the utility model provides an animal trachea perfusion device, its aim at through the light pipe fixed in the catheter, with the leading-in catheter opening part of illumination light, as location signal mark catheter opening position, solve prior art from this and contract through the intubate in-process optic fibre that the optical fiber illumination of nestification in the catheter must appear and return, the inaccurate technical problem in location that leads to.

In order to achieve the above object, according to an aspect of the present invention, there is provided an animal trachea perfusion device, including: the catheter comprises a catheter and a light guide pipe which is nested and fixed with the catheter; one end of the liquid guide pipe is provided with an access port for connection, and the other end of the liquid guide pipe is provided with an opening for forming a drip liquid circulation channel; one end of the light guide pipe is connected with the illumination module through the light guide cone, and the other end of the light guide pipe, which is on the same side with the opening of the liquid guide pipe, is the front end, so that a light signal emitted by the illumination module is transmitted to the end face of the front end of the light guide pipe through the light guide pipe and is used for marking the opening position of the liquid guide pipe.

Preferably, in the animal trachea perfusion device, the catheter extends 1.4-2.7mm outside the light guide tube, the outer side of the extended catheter is nested in the reflector, the distance from the position of the reflector to the tail end of the inserted catheter is 1-2mm, and the reflector is used for reflecting light emitted from the end face of the light guide tube to the side direction.

Preferably, the reflector of the animal trachea perfusion device is a hollow cone, the hollow of the animal trachea perfusion device is matched with the externally-extended catheter, and the cone angle is 90 +/-5 degrees.

Preferably, the animal trachea perfusion device is characterized in that a support tube is wrapped on the outer side of the front section of the light guide tube, the support tube is flush with the end face of the liquid guide tube, and the support tube preferably has a guide angle.

Preferably, the animal trachea perfusion apparatus, the lighting module thereof, comprises a bottom light emitting component; the light emitting part includes a light emitting element and a driving circuit for controlling the brightness and/or switching of the light emitting element.

Preferably, the light-emitting element of the animal trachea perfusion device emits red-near infrared light in the wavelength range of 600-900nm, preferably red light between 600nm and 700 nm.

Preferably, the light emitting elements of the animal trachea perfusion device are uniformly arranged LED lamp beads.

Preferably, the light pipe of the animal trachea perfusion device has a light transmission rate of more than 80% at the wavelength band of 600-900 nm.

Preferably, the light guide pipe of the animal trachea perfusion device comprises an inner layer, a middle layer and an outer layer which are nested tightly from inside to outside, and the optical refractive index of the inner layer and the outer layer of the light guide pipe is smaller than that of the middle layer of the light guide pipe.

Preferably, the animal trachea perfusion device, its leaded light awl is from interior to exterior including closely nested inlayer, intermediate level and skin, leaded light awl inlayer and outer photorefractive index are less than leaded light awl intermediate level, leaded light awl intermediate level with the butt joint of leaded light pipe intermediate level. Generally, through the utility model discloses above technical scheme who conceives compares with prior art, can gain following beneficial effect:

in order to make up the deficiency of the current mouse trachea instillation mode, the utility model provides a brand-new mouse trachea instillation contamination device. The device uses the light guide pipe to transmit the illumination light to replace the prior optical fiber to transmit the illumination light, and the light guide pipe forms a hollow inner cavity and can be nested and fixed with the catheter, thereby fundamentally solving the problems that the light guide device and the catheter can not be relatively fixed and are inevitably retracted to cause inaccurate positioning in the inserting process. The device has no wound on mice, is simple to operate, has high success rate, is economical and portable, can realize repeated exposure of toxicant or medicine for many times, and can accurately control the exposure dose of the toxicant or the medicine in vivo.

In the preferable scheme, the liquid guide pipe extends outwards and is provided with the reflecting mirror, the emitting direction of the marking light is changed from end face emitting and reflecting to side face emitting, the side direction light emitting is uniform, forward light emitting interference is avoided, and the accurate positioning can be realized without specially adjusting the angle of the insertion pipe.

The diameter of the inserted part of the preferred scheme is small, so that the requirement of a slim mouse trachea cannula can be met; the tail end of the intubation tube is combined with a luminous component, and after the intubation tube is inserted into a trachea, the emitted red light-near infrared band light can penetrate through neck muscles and skin to project the position of the thyroid cartilage on the body surface, so that the instillation position is accurately positioned.

Drawings

Fig. 1 is a schematic structural view of an animal trachea perfusion device provided by the utility model;

FIG. 2 is a schematic view of the structure of a light guide tube, a liquid guide tube and a support tube;

fig. 3 is a schematic structural diagram of an illumination module provided in an embodiment of the present invention;

fig. 4 is a schematic view of a light guide front end according to an embodiment of the present invention;

figure 5 is a schematic view of the flaring structure at the upper end of a catheter. .

The same reference numbers will be used throughout the drawings to refer to the same or like elements or structures, wherein: 1 is animal trachea perfusion device, 2 is lighting module, 3 is the access port, 11 are the leaded light awl, 12 are the light pipe, 13 are the catheter, 14 are the speculum, 15 are the stay tube, 121 are the light pipe skin, 122 are the light pipe intermediate level, 123 are the light pipe inlayer, 21 are lighting module and give out light part, 22 are the switch, 23 are the battery, 131 are the flaring of catheter upper end.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. Furthermore, the technical features mentioned in the embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

The utility model provides an animal trachea perfusion device, which comprises a catheter, a light guide pipe, a light guide cone, a support pipe and an illumination module;

the liquid guide pipe is nested and fixed in the light guide pipe, the diameter of the liquid guide pipe is between 0.2 and 0.7mm, one end of the liquid guide pipe is provided with an access port for connection, and the other end of the liquid guide pipe is provided with an opening to form a drip liquid circulation channel; the liquid guide pipe extends 1.4-2.7mm outwards from the light guide pipe, the outer side of the extended liquid guide pipe is nested in the reflector, and the reflector is used for reflecting light emitted by the end face of the light guide pipe into a lateral direction. The reflector is a hollow cone, the hollow part of the reflector is matched with the catheter which extends outwards, the preferable side surface of the reflector is plated with a reflecting film, the cone angle is 90 +/-5 degrees, the preferable 90 degrees, and light emitted from the bottom of the light guide pipe is reflected by the side surface of the reflector and then emitted along the radial direction which is vertical to the inserted catheter; the utility model discloses only catheter head side is luminous, forms clear luminous fine rule, does not give out light forward along the pipe axial to avoided preceding luminous interference to the pipe location, the pipe location is more clear, can satisfy the accurate positioning requirement of mouse intubate. The distance L between the position of the reflector and the tail end of the inserted liquid guide tube is 1-2mm, when the lateral light emitted by the reflector positions the thyroid cartilage, the opening of the liquid guide tube is just 1-2mm below the thyroid cartilage, and the liquid guide tube can be directly instilled; when liquid is instilled into the trachea of the experimental mouse, the inserting catheter is inserted into the trachea of the mouse through the mouth, and the access port is connected with an injector loaded with instillation liquid from the outside.

The wall thickness of the light guide pipe is 0.2mm to 0.4mm, one end of the light guide pipe is connected with the lighting module through a light guide cone, and the other end of the light guide pipe, which is on the same side as the opening of the liquid guide pipe, is the front end; so that the light signal emitted by the lighting module is transmitted to the front end face of the light guide pipe through the light guide pipe and is emitted for marking the opening position of the liquid guide pipe. The light transmission rate of the light guide pipe at the wave band of 600-900nm is more than 80%. The light guide pipe comprises an inner layer, a middle layer and an outer layer which are tightly nested from inside to outside, and the light refractive index of the inner layer and the outer layer of the light guide pipe is smaller than that of the middle layer of the light guide pipe. The light guide cone comprises an inner layer, a middle layer and an outer layer which are tightly nested from inside to outside, the light refractive index of the inner layer and the outer layer of the light guide cone is smaller than that of the middle layer of the light guide cone, the middle layer of the light guide cone is in butt joint with the middle layer of the light guide pipe, and the three-layer structure of the light guide cone is in butt joint with the three-layer structure of the light guide pipe in a matching mode. Due to the principle of total reflection, the light is confined to travel forward in the intermediate layer of the light pipe and the light guide cone. The light guide pipe is preferably made of an organic polymer material and is formed by multilayer extrusion.

The light guide pipe is wrapped with a support pipe, the outer diameter of the support pipe is 0.8-1.5mm, the support pipe is flush with the end face of the liquid guide pipe, and preferably the support pipe is provided with a chamfer. The supporting tube is used for fixing and assembling the reflector and the light guide tube and providing supporting force in the process of inserting the inserted catheter into the mouse trachea; the supporting tube can be made of a capillary glass tube or an acrylic transparent tube or other transparent materials. The supporting tube is only arranged at the front end part, and if a hard tube is integrally sleeved, the supporting tube cannot be inserted in a bending way.

The lighting module comprises a bottom light emitting part, a switch on the side wall and a power supply; the light emitting part comprises a light emitting element and a driving circuit, the driving circuit is used for controlling the brightness and/or the switch of the light emitting element, and the switch is connected with the driving circuit. The light emitting element emits red-near infrared light in the wavelength range of 600-900nm, preferably red light between 600nm-700 nm. The light-emitting elements are LED lamp beads which are uniformly arranged. Based on the light scattering and light absorption spectrum lines of biological tissues, the light-emitting element emits red-near infrared light within the wavelength range of 600-900nm, preferably red light between 600-700 nm, has a relatively obvious marking effect, does not need to expose an air pipe, and can more clearly and directly observe an optical signal for marking; when using a 700-900nm wavelength light source, the light spot is observed by means of an infrared camera or other device. The driving circuit receives signals of the switch to achieve light source lighting, multi-stage or stepless brightness adjustment. The power source is preferably a battery, such as a lithium battery. Preferably, the bottom of the lighting module is packaged by using a transparent high polymer material, and other parts are integrally wrapped by using a waterproof high polymer elastic material, such as silicon rubber; the lighting module is also provided with a charging port, and the charging port is tightly sealed by a rubber plug at ordinary times; the lighting module is suitable for water cleaning, alcohol and ultraviolet disinfection.

The following are examples:

an animal trachea perfusion device comprises a liquid guide pipe, a light guide cone, a support pipe and an illumination module;

the liquid guide pipe is nested and fixed in the light guide pipe, the diameter is 0.3mm, the wall thickness is 0.05mm, one end of the liquid guide pipe is provided with an access port for connection, and the other end of the liquid guide pipe is provided with an opening for forming a drip liquid circulation channel; the liquid guide pipe extends 2mm outwards from the light guide pipe, the outer side of the liquid guide pipe extending outwards is nested in the reflector, and the reflector is used for reflecting light emitted by the end face of the light guide pipe into a lateral direction. The reflector is a hollow cone, the hollow part of the reflector is matched with the liquid guide pipe which extends outwards, the side surface of the reflector is plated with a reflecting film, the conical angle of the reflector is 90 degrees, and light emitted from the bottom of the light guide pipe is reflected by the side surface of the reflector and then is emitted out along the radial direction which is vertical to the inserted liquid guide pipe. The distance L between the position of the reflector and the tail end of the inserted liquid guide tube is 1.5mm, when the lateral light emitted by the reflector positions the thyroid cartilage, the opening of the liquid guide tube is just 1.5mm below the thyroid cartilage, and the liquid guide tube can be directly instilled; in order to improve the connection efficiency of the liquid guide pipe and the access port and ensure the smooth interior of the drip liquid circulation channel, the upper port of the liquid guide pipe is flared, and a flared opening is processed at the upper port of the liquid guide pipe through a thermal flaring process.

The wall thickness of the light guide pipe is 0.3mm, one end of the light guide pipe is connected with the lighting module through a light guide cone, and the other end of the light guide pipe, which is on the same side as the opening of the liquid guide pipe, is dissociated into a front end; so that the light signal emitted by the lighting module is transmitted to the front end face of the light guide pipe through the light guide pipe and is emitted for marking the opening position of the liquid guide pipe. The light transmission rate of the light guide pipe at the wave band of 600-900nm is more than 80%. The light guide pipe comprises an inner layer, a middle layer and an outer layer, wherein the inner layer is tightly nested and made of a fluoroplastic material with the thickness of 0.05mm, the middle layer is made of a polymethyl methacrylate (PMMA) material with the thickness of 0.2mm, the outer layer is made of a fluoroplastic material with the thickness of 0.05mm, and the multiple layers are formed in an extrusion mode. The small head of the light guide cone comprises an inner layer, a middle layer and an outer layer, wherein the inner layer is tightly nested and made of fluoroplastic materials with the thickness of 0.05mm, the middle layer is made of polymethyl methacrylate (PMMA) with the thickness of 0.2mm, and the outer layer is made of fluoroplastic materials with the thickness of 0.05 mm. The big end of the light guide cone comprises an inner layer made of tightly nested 0.05mm thick fluoroplastic material, a middle layer made of 4mm thick polymethyl methacrylate (PMMA) material and an outer layer made of 0.05mm thick fluoroplastic material, and the three-layer structure of the light guide cone and the three-layer structure of the light guide pipe are respectively matched and butted.

The outer side of the light guide pipe is wrapped with a supporting pipe, the outer diameter of the supporting pipe is 1mm, the supporting pipe is flush with the end face of the liquid guide pipe, and the supporting pipe is provided with a chamfer. The supporting tube is used for fixing and assembling the reflector and the light guide tube and providing supporting force in the process of inserting the inserted catheter into the mouse trachea; the supporting tube can be an acrylic transparent tube. The support tube is 3mm long.

The lighting module comprises a bottom light emitting part, a switch on the side wall and a power supply; the light emitting part comprises a light emitting element and a driving circuit, the driving circuit is used for controlling the brightness and/or the switch of the light emitting element, and the switch is connected with the driving circuit. The light emitting element emits red light between 600nm and 700 nm. 650nm LEDs uniformly arranged at the bottom of the light emitting module of the present embodiment are used as light emitting elements. The driving circuit receives signals of the switch to realize multi-level brightness adjustment. The power supply is a lithium battery. The bottom of the illumination module is packaged by using transparent optical silica gel, and other parts are integrally wrapped by using silicon rubber, such as; the lighting module is also provided with a charging port, and the charging port is tightly sealed by a rubber plug at ordinary times; the lighting module is suitable for water cleaning, alcohol and ultraviolet disinfection.

In actual use, a liquid guide pipe, a lighting module and an access port are connected; fixing the mouse after anesthesia, shaving the neck to expose the skin, lighting the lighting module, inserting the insertion catheter into the trachea through the mouse oral cavity, radiating the light radially emitted from the front end of the insertion catheter through neck muscles and the skin, observing the intensity of the body surface light spots with naked eyes, observing no strong body surface projection when the light signals are adjusted to be the strongest light signals, and judging that the catheter is mistakenly inserted into the esophagus by combining the breathing state of the mouse; when the illumination module passes through the thyroid cartilage, the projection of the thyroid cartilage with reduced light intensity can be observed on the body surface, so that the position of the thyroid cartilage is judged, and when a light spot is irradiated on the position of the thyroid cartilage, the opening of the liquid guide pipe is just within the range of 1-2mm below the thyroid cartilage; connecting the syringe loaded with the drip fluid to the access port, slowly injecting the liquid with the specific volume, disconnecting the syringe, and pulling out the insertion catheter; after the test, the whole device can be cleaned by water washing, alcohol sterilization or ultraviolet sterilization.

It will be understood by those skilled in the art that the foregoing is merely a preferred embodiment of the present invention, and is not intended to limit the invention to the particular forms disclosed, but on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention as defined by the appended claims.

Claims (10)

1. An animal tracheal perfusion device, comprising: the device comprises a supporting tube, a liquid guide tube and a light guide tube which is nested and fixed with the liquid guide tube; one end of the liquid guide pipe is provided with an access port for connection, and the other end of the liquid guide pipe is provided with an opening for forming a drip liquid circulation channel; one end of the light guide pipe is connected with the illumination module through the light guide cone, and the other end on the same side with the opening of the liquid guide pipe is a front end, so that a light signal emitted by the illumination module is transmitted to the end face of the front end of the light guide pipe through the light guide pipe and is emitted for marking the opening position of the liquid guide pipe; the outer side of the light pipe is wrapped with a support pipe, and the support pipe is only arranged at the front end part and used for providing support force during the process of inserting the catheter into the trachea of the mouse.

2. An animal tracheal perfusion device as in claim 1, wherein the catheter extends 1.4-2.7mm from the light pipe, the extended catheter is externally nested in a reflector, the reflector is positioned at a distance of 1-2mm from the end inserted into the catheter, and the reflector is used for reflecting the light emitted from the end face of the light pipe to the side direction.

3. An animal tracheal irrigation device as in claim 2 wherein said reflector is a hollow cone, wherein the hollow fits said overhanging catheter, and the cone angle is 90 ± 5 °.

4. An animal tracheal irrigation device as in claim 2 wherein the support tube is flush with the catheter end face, the support tube having a lead angle.

5. The animal tracheal perfusion apparatus of claim 1, wherein the illumination module comprises a bottom light emitting component; the light emitting part includes a light emitting element and a driving circuit for controlling the brightness and/or switching of the light emitting element.

6. The animal tracheal perfusion device of claim 5, wherein the light emitting element emits red-near infrared light in the wavelength range of 600-900 nm.

7. The animal tracheal perfusion device of claim 5, wherein the light emitting elements are uniformly arranged LED beads.

8. The animal tracheal perfusion device of claim 1, wherein the light pipe has a light transmittance of 80% or more at the 600-900nm band.

9. The animal tracheal perfusion apparatus of claim 1, wherein the light pipe comprises, from inside to outside, a tightly nested inner layer, an intermediate layer, and an outer layer, the inner and outer layers of the light pipe having a lower optical refractive index than the intermediate layer of the light pipe.

10. The animal tracheal perfusion apparatus of claim 9, wherein the light guide cone comprises, from inside to outside, a tightly nested inner layer, an intermediate layer, and an outer layer, the inner and outer layers of the light guide cone having a lower optical refractive index than the intermediate layer of the light guide cone, the intermediate layer of the light guide cone interfacing with the intermediate layer of the light guide tube.

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