CN210856119U

CN210856119U - Monocyte separating tube

Info

Publication number: CN210856119U
Application number: CN201921418040.2U
Authority: CN
Inventors: 万智恒; 张称; 张国辉
Original assignee: Haolin Tianjin Biotechnology Co ltd
Current assignee: Haolin Tianjin Biotechnology Co ltd
Priority date: 2019-08-29
Filing date: 2019-08-29
Publication date: 2020-06-26
Anticipated expiration: 2029-08-29

Abstract

The utility model discloses a monocyte separating tube, including test tube body, test tube lid, separation glue, sealing mechanism and density gradient liquid, the inside bottom of test tube body is provided with density gradient liquid, and the test tube body internally mounted of density gradient liquid top has separation glue to the top of separation glue is provided with the sodium citrate layer, the top of test tube body is provided with the rubber buffer, and is provided with the annular groove on the test tube body inner wall in the rubber buffer outside to the inside of annular groove is provided with annular sand grip, the inside of test tube body is provided with antibiotic stopper, and the below of antibiotic stopper is provided with the syringe needle to the top of syringe needle extends to the top of antibiotic stopper, the top of antibiotic stopper is provided with sealing mechanism. The utility model discloses not only avoided the needle tubing to bring the bacterium into the inside of test tube body, realized the independent function of placing of test tube body, guaranteed the aseptic ization of experimentation moreover.

Description

Monocyte separating tube

Technical Field

The utility model relates to a medical science research utensil technical field specifically is a monocyte separator tube.

Background

In clinical medical examination, the examination aiming at specific cell functions and biological characteristics often involves a cell separation technology, the separation of mononuclear cells from peripheral blood and various body fluids is an important pretreatment step for lymphocyte immunological research and cytological experiments in vitro, and the quality and quantity of separated target cells are important links for ensuring the reliability of subsequent experiments, so that a monocyte separation tube is required to be used for separation operation.

The existing monocyte separation tube on the market has various types, can basically meet the use requirements of people, but still has certain defects, and has the following specific problems.

(1) When the traditional monocyte separation tube is used for sampling, bacteria are easily entrained by an external needle head to enter the inside of the separation tube, so that the sample in the separation tube is polluted;

(2) when the traditional monocyte separation tube is used, the function of independently placing the monocyte separation tube on a test bed is usually lacked, so that the separation tube is easy to topple, and the experimental process is influenced;

(3) the traditional monocyte separation tube lacks an independent sealing cover when in use, so that foreign matters are easy to enter the tube, and the aseptic operation is difficult to realize.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a monocyte separator tube to it smugglies to smuggle the bacterium easily secretly to get into the separator tube inside and causes the pollution to propose the outside syringe needle of monocyte separator tube among the above-mentioned background art, lacks the function of independently placing on the test bench and is difficult to realize the problem of aseptic ization operation.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a monocyte separator tube, includes test tube body, test tube lid, separation glue, sealing mechanism and density gradient liquid, the inside bottom of test tube body is provided with density gradient liquid, and the test tube body internally mounted of density gradient liquid top has the separation glue to the top of separation glue is provided with the sodium citrate layer, the inside of test tube body is provided with antibiotic stopper, and the below of antibiotic stopper is provided with the syringe needle to the top of syringe needle extends to the top of antibiotic stopper, the top of antibiotic stopper is provided with sealing mechanism, be fixed with the scale mark on the outer wall of test tube body one side.

Preferably, the top of test tube body is provided with the rubber buffer, and is provided with annular groove on the test tube body inner wall in the rubber buffer outside to annular groove's inside is provided with annular sand grip, annular sand grip's one end and rubber buffer fixed connection.

Preferably, the test tube lid is sleeved on the top of the test tube body, and sealed glue is installed to the bottom of test tube lid, and sealed glue closely laminates with the top of test tube body, sealed bottom and rubber buffer fixed connection of gluing.

Preferably, the cover is equipped with the rubber lantern ring on the outer wall of test tube body, and all is fixed with spacing lug on the outer wall of rubber lantern ring both sides, and the outside of rubber lantern ring is provided with spacing sleeve, and spacing telescopic external fixation has fixed support body, and fixed support body and spacing lug are mutually supported to the sucking disc is all installed to the bottom of fixed support body.

Preferably, the sealing mechanism is internally provided with a rotary retaining plate, a rotating handle and a rotating shaft in sequence, and the top end of the antibacterial plug at the position of the needle head is provided with the rotary retaining plate.

Preferably, one side on rotatory separation blade top is fixed with the turning handle, and one side that the turning handle was kept away from to rotatory separation blade is fixed with the pivot, and the bottom and the antibiotic stopper of pivot are articulated mutually.

Compared with the prior art, the beneficial effects of the utility model are that: the monocyte separation tube not only prevents bacteria from being brought into the tube body of the test tube by the needle tube, realizes the independent placement function of the tube body of the test tube, but also ensures the sterility of the test process;

(1) through the arrangement of the rotary retaining plate, the rotary handle, the rotary shaft, the antibacterial plug and the needle head, the rotary handle is operated to drive the rotary retaining plate to rotate under the hinging action of the rotary shaft, and the external needle tube is connected with the needle head, so that the inside of the tube body of the test tube is isolated from the outside air, and bacteria are prevented from being brought into the inside of the tube body of the test tube by the needle tube;

(2) the test tube body is limited inside the limiting sleeve by the rubber sleeve ring on the outer wall of the test tube body under the action of the limiting lug, and the test tube body is fixed on the test bed by pressing the fixing frame body to apply force and the sucking disc, so that the independent placement function of the test tube body is realized;

(3) through being provided with test tube lid, annular groove, sealed glue, rubber buffer and annular sand grip, press the test tube lid and make annular sand grip and the annular groove block on the rubber buffer outer wall to make sealed glue laminate with the top of test tube body, under the double seal structure effect of test tube lid, guaranteed the aseptic ization of experimentation.

Drawings

Fig. 1 is a schematic front view of a cross-sectional structure of the present invention;

FIG. 2 is a schematic view of the cross-sectional structure of the tube body of the test tube of the present invention;

FIG. 3 is a schematic view of the top view, cross section and enlarged structure of the tube body of the present invention;

fig. 4 is an enlarged schematic structural view of the sealing mechanism of the present invention.

In the figure: 1. a test tube body; 2. a suction cup; 3. a fixed frame body; 4. a rubber collar; 5. a test tube cover; 6. scale marking; 7. a limiting sleeve; 8. a limiting bump; 9. separating the gel; 10. a sodium citrate layer; 11. an antibacterial plug; 12. an annular groove; 13. sealing glue; 14. a rubber plug; 15. an annular rib; 16. a sealing mechanism; 1601. rotating the baffle plate; 1602. a handle is rotated; 1603. a rotating shaft; 17. a needle head; 18. density gradient liquid.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides an embodiment: a monocyte separation tube comprises a test tube body 1, a test tube cover 5, separation gel 9, a sealing mechanism 16 and density gradient liquid 18, wherein the density gradient liquid 18 is arranged at the bottom end inside the test tube body 1, the separation gel 9 is arranged inside the test tube body 1 above the density gradient liquid 18, and a sodium citrate layer 10 is arranged above the separation gel 9;

a rubber plug 14 is arranged at the top end of the top of the test tube body 1, an annular groove 12 is formed in the inner wall of the test tube body 1 outside the rubber plug 14, an annular convex strip 15 is arranged inside the annular groove 12, one end of the annular convex strip 15 is fixedly connected with the rubber plug 14, a test tube cover 5 is sleeved at the top end of the test tube body 1, a sealant 13 is arranged at the bottom end of the test tube cover 5, the sealant 13 is tightly attached to the top end of the test tube body 1, and the bottom end of the sealant 13 is fixedly connected with the rubber plug 14 and used for guaranteeing the sterility of the test tube body;

the outer wall of the test tube body 1 is sleeved with a rubber sleeve ring 4, the outer walls of two sides of the rubber sleeve ring 4 are both fixed with a limiting lug 8, the outer side of the rubber sleeve ring 4 is provided with a limiting sleeve 7, the outer part of the limiting sleeve 7 is fixed with a fixing frame body 3, the fixing frame body 3 is matched with the limiting lug 8, and the bottom end of the fixing frame body 3 is provided with a sucker 2 for realizing the independent placement function of the test tube body 1;

an antibacterial plug 11 is arranged inside the test tube body 1, a needle head 17 is arranged below the antibacterial plug 11, the top end of the needle head 17 extends to the top end of the antibacterial plug 11, a sealing mechanism 16 is arranged at the top end of the antibacterial plug 11, and a scale mark 6 is fixed on the outer wall of one side of the test tube body 1;

a rotary baffle 1601, a rotating handle 1602 and a rotating shaft 1603 are sequentially arranged in the sealing mechanism 16, the rotary baffle 1601 is arranged at the top end of the antibacterial plug 11 at the position of the needle 17, the rotating handle 1602 is fixed on one side of the top end of the rotary baffle 1601, the rotating shaft 1603 is fixed on one side of the rotary baffle 1601, which is far away from the rotating handle 1602, and the bottom end of the rotating shaft 1603 is hinged to the antibacterial plug 11;

when needs sample, staff's operation turning handle 1602 makes it drive rotatory separation blade 1601 rotatory under the articulated effect of pivot 1603, is connected outside needle tubing and syringe needle 17, accomplishes the sample work, and the back is accomplished in the sample, separates outside needle tubing and syringe needle 17, and rotatory rotation separation blade 1601 makes it close with the same reason for the inside and the outside air of test tube body 1 keep apart, thereby has avoided the needle tubing to bring the bacterium into the inside of test tube body 1.

The working principle is as follows: when the test tube body 1 is used, a worker firstly puts a blood sample into the test tube body 1, secondly plugs the antibacterial plug 11 into the test tube body 1, and centrifuges the test tube body 1 through a centrifuge to enable the blood sample in the test tube body 1 to be analyzed and layered, then places the test tube body 1 into the limiting sleeve 7, at the moment, the rubber lantern ring 4 on the outer wall of the test tube body 1 limits the test tube body 1 into the limiting sleeve 7 under the action of the limiting lug 8, and fixes the test tube body 1 on a test bench through pressing the fixing frame body 3 and the sucker 2, so that the independent placement function of the test tube body 1 is realized, the phenomenon that the test tube body 1 is toppled over is avoided, when sampling is needed, the worker operates the rotating handle 1602 to drive the rotating block 1601 to rotate under the hinging action of the rotating shaft 1603, and connects the outer part of the needle tube with the needle 17, accomplish the sample work, the sample is accomplished the back, with outside needle tubing and syringe needle 17 separation, rotatory rotation catch 1601 makes it close with the reason, make test tube body 1's inside and outside air keep apart, thereby avoided the needle tubing to bring the bacterium into test tube body 1's inside, and finally, when there is no experimental operation temporarily, press test tube lid 5 and make annular sand grip 15 and annular groove 12 block on the rubber buffer 14 outer wall, and make sealed glue 13 and test tube body 1's top laminating, under test tube lid 5's double-deck seal structure effect, the aseptic ization of experimentation has been guaranteed, accomplish the work of monocyte separator tube.

It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. The utility model provides a monocyte separation tube, includes test tube body (1), test tube lid (5), separation glue (9), sealing mechanism (16) and density gradient liquid (18), its characterized in that: the bottom of test tube body (1) inside is provided with density gradient liquid (18), and test tube body (1) internally mounted of density gradient liquid (18) top has separation glue (9) to the top of separation glue (9) is provided with sodium citrate layer (10), the inside of test tube body (1) is provided with antibiotic stopper (11), and the below of antibiotic stopper (11) is provided with syringe needle (17) to the top of syringe needle (17) extends to the top of antibiotic stopper (11), the top of antibiotic stopper (11) is provided with sealing mechanism (16), be fixed with scale mark (6) on the outer wall of test tube body (1) one side.

2. The monocyte-separating tube of claim 1, wherein: the top of test tube body (1) is provided with rubber buffer (14), and is provided with annular groove (12) on test tube body (1) inner wall in the rubber buffer (14) outside to the inside of annular groove (12) is provided with annular sand grip (15), the one end and rubber buffer (14) fixed connection of annular sand grip (15).

3. A monocyte-separating tube as claimed in claim 2, wherein: test tube body (1) top cover is equipped with test tube lid (5), and the bottom of test tube lid (5) installs sealed glue (13), sealed glue (13) and the top of test tube body (1) closely laminate, the bottom and rubber buffer (14) fixed connection of sealed glue (13).

4. The monocyte-separating tube of claim 1, wherein: the outer wall of test tube body (1) is gone up the cover and is equipped with rubber sleeve ring (4), and all is fixed with spacing lug (8) on the outer wall of rubber sleeve ring (4) both sides, the outside of rubber sleeve ring (4) is provided with spacing sleeve (7), and the external fixation of spacing sleeve (7) has fixed support body (3), and fixed support body (3) mutually support with spacing lug (8) to sucking disc (2) are all installed to the bottom of fixed support body (3).

5. The monocyte-separating tube of claim 1, wherein: the sealing mechanism (16) is internally provided with a rotary baffle (1601), a rotating handle (1602) and a rotating shaft (1603) in sequence, and the top end of the antibacterial plug (11) at the position of the needle head (17) is provided with the rotary baffle (1601).

6. The monocyte-separating tube of claim 5, wherein: one side on rotatory separation blade (1601) top is fixed with turning handle (1602), and rotatory separation blade (1601) keep away from one side of turning handle (1602) and be fixed with pivot (1603), and the bottom and the antibiotic stopper (11) of pivot (1603) are articulated mutually.