CN210855918U - Chromatography device for separating gene recombinant albumin - Google Patents

Abstract

The utility model relates to a chromatography technical field specifically is a gene recombination albumin is chromatographic device for separation, including chromatography column support and chromatography column group, the chromatography column support includes base, main tributary pole and collar, and the chromatography column group includes chromatography column, lower floor's chromatography column and accuse liquid carousel, goes up the chromatography column and inserts perpendicularly and establish in the collar in the top, and lower floor's chromatography column inserts perpendicularly and establishes in the collar in the below, goes up and is connected through accuse liquid carousel between the lower extreme of chromatography column and the upper end of lower floor's chromatography column, and beneficial effect is: the utility model discloses a control air pump work, the air pump passes through the air duct and pressurizes along the connector that admits air to last chromatographic column inside, makes during the inside sample solution of chromatographic column is extruded fast and enters into lower floor's analytical column, and the control flowing back valve is opened, can discharge the inside sample solution of lower floor's analytical column along the fluid-discharge tube.

Description

Chromatography device for separating gene recombinant albumin

Technical Field

The utility model relates to a chromatography technical field specifically is a gene recombination is chromatography device for albumin separation.

Background

Affinity chromatography in the chromatographic technique is widely applied to biomolecule separation and analysis, can efficiently and quickly separate a target object or remove impurities from a mixture, and when a sample solution passes through the chromatographic column, the target object is specifically combined with the affinity filler in the chromatographic column and stays in the chromatographic column, other unadsorbed molecules directly flow out, and the chromatographic column can be eluted by selecting a proper solution to elute the combined protein, so that the separation of the target molecule is realized.

The traditional fixed bed intermittent operation mode is adopted, the operation is simple, but the ubiquitous carrier utilization rate is low, the effect of adsorbing a target object by the filler in the chromatographic column is poor, the flow rate of the chromatographic column is low, the flux is small, and the purification and chromatography efficiency is low.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a gene recombination is chromatographic device for albumin separation to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a chromatography device for separating gene recombinant albumin comprises a chromatography column support and a chromatography column group, wherein the chromatography column support comprises a base, a main supporting rod and a mounting ring, the upper surface of the base is welded with the main supporting rod which is vertically arranged, the main supporting rod is welded with two horizontal rods which are horizontally arranged, the end parts of the two horizontal rods are welded with the mounting ring, the chromatography column group comprises an upper chromatography column, a lower chromatography column and a liquid control turntable, the upper chromatography column is vertically inserted into the mounting ring above, the lower chromatography column is vertically inserted into the mounting ring below, the lower end of the upper chromatography column is connected with the upper end of the lower chromatography column through the liquid control turntable, the upper port of the upper chromatography column is hermetically connected with an upper chromatography plunger, the middle part of the upper chromatography plunger is provided with an air inlet, the air inlet is connected with an air inlet joint through threads, one side of the upper end of the upper chromatography column is provided with a liquid inlet joint, the lower, the middle part of the liquid discharge pipe is provided with a liquid discharge valve.

Preferably, the inner wall of the mounting ring is adhered with an elastic rubber layer, and the elastic rubber layer is tightly attached to the outer walls of the upper chromatographic column and the lower chromatographic column.

Preferably, the lower end of the upper chromatographic column is rotatably connected with the upper part of the liquid control turntable, the lower part of the liquid control turntable is rotatably connected with a lower-layer analytical plunger, and the lower-layer analytical plunger is inserted into an upper port of the lower chromatographic column.

Preferably, one side of the bottom of the upper chromatographic column is provided with a liquid guiding through hole, the partition plate inside the liquid control turntable and the lower chromatographic plunger are provided with corresponding liquid guiding through holes, and the outer wall of the liquid control turntable is provided with anti-skid friction lines.

Preferably, the inner port close to the liquid inlet joint is provided with a sealing sheet, the sealing sheet is welded with an elastic sheet, and the elastic sheet is inserted on the inner wall of the upper chromatographic column.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the liquid control turntable is rotated, so that the liquid guide through holes arranged on the internal partition plate of the liquid control turntable are staggered with the liquid guide through holes at the bottom of the upper chromatographic column, and then the target object in the sample solution is fully adsorbed by the filler in the upper chromatographic column;

2. the utility model discloses a control air pump work, the air pump passes through the air duct and pressurizes along the connector that admits air to last chromatographic column inside, makes during the inside sample solution of chromatographic column is extruded fast and enters into lower floor's analytical column, and the control flowing back valve is opened, can discharge the inside sample solution of lower floor's analytical column along the fluid-discharge tube.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a cross-sectional view of the structure of the present invention;

FIG. 3 is a front sectional view of the structure of the present invention

Fig. 4 is an enlarged view of a portion a in fig. 3.

In the figure: the device comprises a chromatographic column support 1, a chromatographic column group 2, a base 3, a main support rod 4, a mounting ring 5, an upper chromatographic column 6, a lower chromatographic column 7, a liquid control rotary disc 8, a lower chromatographic plunger 81, an upper chromatographic plunger 9, an air inlet joint 10, a liquid inlet joint 11, a liquid discharge pipe 12, a liquid discharge valve 13, a sealing sheet 14, an elastic sheet 15 and a liquid guide through hole 16.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1 to 4, the present invention provides a technical solution: the utility model provides a gene recombination albumin is chromatographic device for separation, combine shown in fig. 1 and fig. 3, including chromatography column support 1 and chromatography column group 2, chromatography column support 1 includes base 3, main tributary pole 4 and collar 5, the last skin weld of base 3 has a perpendicular main tributary pole 4 that sets up, two horizontal poles that the level that sets up are welded on main tributary pole 4, the tip welding of two horizontal poles has collar 5, chromatography column group 2 includes chromatography column 6, lower floor's chromatography column 7 and accuse liquid carousel 8, go up chromatography column 6 and insert perpendicularly and establish in the collar 5 of top, lower chromatography column 7 is inserted perpendicularly and is established in the collar 5 of below, the inner wall bonding elasticity rubber layer of collar 5, and the outer wall of upper chromatography column 6, lower chromatography column 7 is hugged closely to elasticity rubber layer, guarantee collar 5 to the fixed of upper chromatography column 6, lower chromatography column 7.

With reference to fig. 2 and 3, the lower end of the upper chromatographic column 6 is connected to the upper end of the lower chromatographic column 7 through a liquid control rotating disc 8, specifically: the lower extreme of going up chromatographic column 6 rotates with the upper portion of accuse liquid carousel 8 to be connected, the lower part of accuse liquid carousel 8 rotates and is connected with lower floor and analyzes plunger 81, lower floor is analyzed plunger 81 and is fixed to be inserted and establish the last port of chromatographic column 7 down, drain through-hole 16 has been seted up to the bottom one side of going up chromatographic column 6, corresponding drain through-hole 16 has been seted up to the inside baffle of accuse liquid carousel 8 and lower floor and analyze plunger 81, through rotating accuse liquid carousel 8, make the bottom of going up chromatographic column 6, when baffle inside of accuse liquid carousel 8 and lower floor analyze plunger 81 and set up corresponding drain through-hole 16 and communicate with each other, then go up the inside sample solution of chromatographic column 6 and enter into the inside of lower floor's analytic column 7, the outer wall of accuse liquid carousel 8 is provided with anti-skidding friction line, increase the frictional force when rotating accuse liquid carousel.

An upper port of an upper chromatographic column 6 is connected with an upper chromatographic plunger 9 through thread sealing, the middle part of the upper chromatographic plunger 9 is provided with an air inlet, the air inlet is connected with an air inlet joint 10 through threads, the air inlet joint 10 is connected with an air pump through an air duct, the air pump pressurizes the interior of the upper chromatographic column 6 along the air inlet joint 10 through the air duct, so that a sample solution in the upper chromatographic column 6 is quickly extruded into a lower chromatographic column 7, and a sample solution in the lower chromatographic column 7 is discharged along a liquid discharge pipe 12, one side of the upper end of the upper chromatographic column 6 is provided with a liquid inlet joint 11, the liquid inlet joint 11 is used for connecting the liquid inlet pipe and is used for filling the sample solution into the upper chromatographic column 6, as shown in a combined graph 3 and a graph 4, a sealing sheet 14 is arranged at an inner port close to the liquid inlet joint 11, an elastic sheet 15 is welded on the sealing sheet 14, the elastic sheet 15 is, originally, the gasket 14 of sealed feed liquor splice 11 internal port is backed up open for sample solution enters into the inside of last chromatography column 6, and when the air pump let in gas to last chromatography column 6, gasket 14 hugs closely sealed feed liquor splice 11's internal port under the flexure strip 15 kick-backs, prevent that the inside gas of last chromatography column 6 from leaking along feed liquor splice 11, as shown in fig. 3, the lower port of lower chromatography column 7 is equipped with fluid-discharge tube 12, the middle part of fluid-discharge tube 12 is equipped with flowing back valve 13, open through controlling flowing back valve 13, can discharge the inside sample solution of lower chromatography column 7 along fluid-discharge tube 12.

The working principle is as follows: the utility model is operated, the upper chromatographic column 6 and the lower chromatographic column 7 are respectively filled with the filler specifically combined with the target object, the liquid inlet joint 11 is connected with the liquid inlet pipe for filling the sample solution into the upper chromatographic column 6, the liquid control rotary disc 8 is rotated to ensure that the liquid guide through hole 16 arranged on the inner partition plate of the liquid control rotary disc 8 is staggered with the liquid guide through hole 16 at the bottom of the upper chromatographic column 6, then the target object in the sample solution is fully absorbed by the filler in the upper chromatographic column 6, the liquid control rotary disc 8 is rotated to ensure that the bottom of the upper chromatographic column 6, the partition plate in the liquid control rotary disc 8 and the lower chromatographic column 81 are provided with the corresponding liquid guide through holes 16 to be communicated with each other, then the sample solution in the upper chromatographic column 6 enters the lower chromatographic column 7, at the moment, the air pump is controlled to work, the air pump pressurizes the inner part of the upper chromatographic column 6 along the air inlet joint 10 through the air guide pipe, the sample solution in the upper chromatographic column 6 is rapidly extruded into the lower chromatographic column 7, the flow rate of the sample solution is increased, and the sample solution in the lower chromatographic column 7 can be rapidly discharged along the liquid discharge pipe 12 by controlling the opening of the liquid discharge valve 13.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (5)

1. The utility model provides a gene recombination is chromatographic device for albumin separation, includes chromatographic column support (1) and chromatographic column group (2), its characterized in that: the chromatographic column support (1) comprises a base (3), a main supporting rod (4) and a mounting ring (5), wherein the upper surface of the base (3) is welded with the vertically arranged main supporting rod (4), the upper part of the main supporting rod (4) is welded with two horizontally arranged cross rods, the end parts of the two cross rods are welded with the mounting ring (5), the chromatographic column group (2) comprises an upper chromatographic column (6), a lower chromatographic column (7) and a liquid control turntable (8), the upper chromatographic column (6) is vertically inserted into the upper mounting ring (5), the lower chromatographic column (7) is vertically inserted into the lower mounting ring (5), the lower end of the upper chromatographic column (6) is connected with the upper end of the lower chromatographic column (7) through the liquid control turntable (8), the upper end opening of the upper chromatographic column (6) is hermetically connected with an upper chromatographic plunger (9), the middle part of the upper chromatographic plunger (9) is provided with an air inlet, and the air inlet is connected with an air inlet joint (10) through threads, one side of the upper end of the upper chromatographic column (6) is provided with a liquid inlet joint (11), the lower port of the lower chromatographic column (7) is provided with a liquid discharge pipe (12), and the middle part of the liquid discharge pipe (12) is provided with a liquid discharge valve (13).

2. The chromatography device for separating recombinant albumin according to claim 1, wherein: the inner wall of the mounting ring (5) is adhered with an elastic rubber layer, and the elastic rubber layer is tightly attached to the outer walls of the upper chromatographic column (6) and the lower chromatographic column (7).

3. The chromatography device for separating recombinant albumin according to claim 1, wherein: the lower end of the upper chromatographic column (6) is rotatably connected with the upper part of the liquid control turntable (8), the lower part of the liquid control turntable (8) is rotatably connected with a lower chromatography plunger (81), and the lower chromatography plunger (81) is inserted into the upper port of the lower chromatographic column (7).

4. The chromatography device for separating recombinant albumin according to claim 1, wherein: one side of the bottom of the upper chromatographic column (6) is provided with a liquid guiding through hole (16), a partition plate inside the liquid control turntable (8) and the lower chromatographic plunger (81) are provided with corresponding liquid guiding through holes (16), and the outer wall of the liquid control turntable (8) is provided with anti-skid friction lines.

5. The chromatography device for separating recombinant albumin according to claim 1, wherein: and a sealing sheet (14) is arranged at the inner port tightly attached to the liquid inlet joint (11), an elastic sheet (15) is welded on the sealing sheet (14), and the elastic sheet (15) is inserted on the inner wall of the upper chromatographic column (6).

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