CN210841049U - A culture medium adds device for blueberry tissue culture - Google Patents

A culture medium adds device for blueberry tissue culture Download PDF

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Publication number
CN210841049U
CN210841049U CN201921737847.2U CN201921737847U CN210841049U CN 210841049 U CN210841049 U CN 210841049U CN 201921737847 U CN201921737847 U CN 201921737847U CN 210841049 U CN210841049 U CN 210841049U
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CN
China
Prior art keywords
culture
tank
tank body
culture medium
infusion
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Expired - Fee Related
Application number
CN201921737847.2U
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Chinese (zh)
Inventor
陈芳芳
陈相涛
胡重九
王方
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Hangzhou Mumu Biotechnology Co Ltd
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Hangzhou Mumu Biotechnology Co Ltd
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Priority to CN201921737847.2U priority Critical patent/CN210841049U/en
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Abstract

The utility model discloses a culture medium adding device for blueberry tissue culture, which comprises a liquid storage tank, an infusion catheter and a culture tank, wherein the upper end surface of the liquid storage tank is connected with the infusion catheter, the right lower end of the infusion catheter is connected with the culture tank, the liquid storage tank comprises a first tank body, a sealing plug and a liquid outlet cylinder, the upper end of the first tank body is provided with the sealing plug, the upper end of the sealing plug is provided with the liquid outlet cylinder, and the right side of the upper end surface of the liquid outlet cylinder is connected with the infusion catheter; the infusion catheter comprises an infusion tube and a water pump, and the right end of the infusion tube is communicated with the inner cavity of the culture tank; the culture tank comprises a rubber plug and a second tank body, and the rubber plug is fixedly arranged at the upper end of the second tank body. The adding device solves the technical problems of structural connection and integration with a control system in the culture medium adding device through the installation and connection of the liquid storage tank, the infusion catheter and the culture tank.

Description

A culture medium adds device for blueberry tissue culture
Technical Field
The utility model relates to a culture medium technical field specifically is a culture medium interpolation device for blueberry tissue culture.
Background
The main steps of the process of culturing the blueberry tissues difficult to culture are that the blueberry tissues are inoculated in a small amount of liquid culture medium and are grown and propagated in a small space, the liquid culture medium is gradually added under the condition of growing and propagating to a certain abundance, and the growing space of the blueberry tissues is continuously expanded. In the process of blueberry tissue culture, especially in the process of adding a liquid culture medium, blueberry tissues growing in the original growth space are easily polluted by other microorganisms, so that the process of culturing rare blueberry tissues in an expansion process often fails, and the culture process is long, the cost of the culture medium is high, and further economic and labor losses are caused.
The inventor finds that the existing culture medium adding device for blueberry tissue culture is slightly simple in design structure, low in adding efficiency, less in intelligent application, easy to be polluted by other microorganisms in the adding process, incapable of providing proper culture temperature and environment, poor in sealing effect, lack of temperature control equipment, poor in strength, heat preservation, heat insulation and waterproof sealing performance of the device body, and incapable of effectively observing the culture condition inside the device.
Aiming at the problems and challenges, the culture medium adding device for blueberry tissue culture has the advantages that the adding efficiency is high, the sensing equipment and the control equipment are combined, the culture medium adding device is not easily polluted by other microorganisms in the adding process, the proper culture temperature and environment can be provided, the sealing effect of the device is good, the temperature control equipment is added, the strength, the heat preservation, the heat insulation and the waterproof sealing performance of the device body are good, the culture condition in the device can be effectively observed, and the like.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a culture medium adding device for blueberry tissue culture, which has the advantages of higher adding efficiency, combination of sensing equipment and control equipment, less possibility of being polluted by other microorganisms in the adding process, capability of providing proper culture temperature and environment, good sealing effect of the device, addition of temperature control equipment, better strength, heat preservation, heat insulation and waterproof sealing performance of the device body, capability of effectively observing the culture condition in the device and the like, thereby solving the problems that the traditional culture medium adding device for blueberry tissue culture is slightly simple in design structure, lower adding efficiency, less intelligent application, easy pollution by other microorganisms in the adding process, incapability of providing proper culture temperature and environment, poor sealing effect of the device, lack of temperature control equipment, poorer strength, heat preservation, heat insulation and waterproof sealing performance of the device body, the culture inside the apparatus cannot be observed effectively.
In order to achieve the above object, the utility model provides a following technical scheme: a culture medium adding device for blueberry tissue culture comprises a liquid storage tank, an infusion guide pipe and a culture tank, wherein the upper end face of the liquid storage tank is connected with the infusion guide pipe, the right lower end of the infusion guide pipe is connected with the culture tank, the liquid storage tank comprises a first tank body, a sealing plug and a liquid outlet cylinder, a refrigerator and a heater are respectively arranged in an inner cavity of the first tank body, the rear end of the refrigerator is electrically connected with a driving motor, and the heater is fixedly arranged at the right end of the inner wall of the first tank body; the upper end of the first tank body is provided with a sealing plug, the upper end of the sealing plug is provided with a liquid outlet cylinder, and the right side of the upper end surface of the liquid outlet cylinder is connected with a transfusion catheter;
the infusion catheter comprises an infusion tube and a water pump, the water pump is arranged on the outer wall of the infusion tube, and the right end of the infusion tube is communicated with the inner cavity of the culture tank;
the culture tank comprises a rubber plug and a second tank body, and the rubber plug is fixedly arranged at the upper end of the second tank body.
Further, be equipped with observation porthole and temperature monitor on the preceding terminal surface of the first jar of body, temperature monitor's right-hand member is installed temperature regulation button.
Further, a temperature sensor is fixedly arranged on the inner wall of the first tank body and is electrically connected with a temperature display.
Further, the mounting groove has been seted up to the up end of the first jar of body, and evenly scribbles anti-skidding sealing material on the inner wall of mounting groove.
Furthermore, the lower end of the infusion tube is provided with a plurality of through holes, and the diameter of each through hole is one sixth of the diameter of the infusion tube.
Furthermore, protective layers are arranged on the inner walls of the first tank body and the second tank body, each protective layer consists of a heat insulation layer, a reinforcing layer and a waterproof sealing layer, and the reinforcing layers are arranged in interlayers between the heat insulation layers and the waterproof sealing layers.
Compared with the prior art, the beneficial effects of the utility model are as follows:
1. this a culture medium interpolation device for blueberry tissue culture through the installation and the connection of depositing fluid reservoir, infusion pipe and culture tank, has solved each structural connection and the technical problem of control system integration in the culture medium interpolation device.
2. The culture medium adding device for blueberry tissue culture achieves the purpose of sealing the device through the installation of the sealing plug and the anti-skidding sealing material, and avoids the influence on the culture effect due to the interference of other external microorganisms; through the connection of temperature sensor and temperature monitor, reach the purpose of in time mastering the cultivation temperature in the device, also be convenient for provide a suitable temperature, improve the quality of cultivateing.
3. According to the culture medium adding device for blueberry tissue culture, the liquid culture medium adding speed of the device can be effectively increased through the installation of the through holes and the water pump, so that the working efficiency of the device is improved; through the installation of protective layer, promoted and added the inside intensity of device, heat preservation thermal-insulated and waterproof sealing performance, more can adapt to complicated interpolation environment, prolonged the life of device, the cost is reduced.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the split structure of the liquid storage tank of the present invention;
fig. 3 is a schematic view of the internal plane structure of the present invention;
fig. 4 is an enlarged schematic view of the structure at a of the present invention;
fig. 5 is a schematic view of the internal cross-sectional structure of the protective layer of the present invention.
In the figure: 1. a liquid storage tank; 11. a first tank; 111. a refrigerator; 1111. a drive motor; 112. a heater; 113. an observation porthole; 114. a temperature display; 1141. a temperature adjustment button; 115. a temperature sensor; 116. mounting grooves; 1161. an anti-slip sealing material; 12. a sealing plug; 13. a liquid outlet cylinder; 2. an infusion catheter; 21. a transfusion tube; 211. a through hole; 22. a water pump; 3. a culture tank; 31. a rubber plug; 32. a second tank; 4. a protective layer; 41. a heat insulation layer; 42. a reinforcing layer; 43. and (4) a waterproof sealing layer.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-3, a culture medium adding device for blueberry tissue culture comprises a liquid storage tank 1, an infusion catheter 2 and a culture tank 3, wherein the upper end surface of the liquid storage tank 1 is connected with the infusion catheter 2, the right lower end of the infusion catheter 2 is connected with the culture tank 3, the liquid storage tank 1 comprises a first tank body 11, a sealing plug 12 and a liquid outlet cylinder 13, an inner cavity of the first tank body 11 is respectively provided with a refrigerator 111 and a heater 112, the rear end of the refrigerator 111 is electrically connected with a driving motor 1111, and the heater 112 is fixedly arranged at the right end of the inner wall of the first tank body 11; a sealing plug 12 is arranged at the upper end of the first tank body 11, a liquid outlet cylinder 13 is arranged at the upper end of the sealing plug 12, and the right side of the upper end surface of the liquid outlet cylinder 13 is connected with a transfusion catheter 2; the infusion catheter 2 comprises an infusion tube 21 and a water pump 22, the water pump 22 is arranged on the outer wall of the infusion tube 21, and the right end of the infusion tube 21 is communicated with the inner cavity of the culture tank 3; the culture tank 3 comprises a rubber plug 31 and a second tank body 32, and the rubber plug 31 is fixedly arranged at the upper end of the second tank body 32; an observation porthole 113 and a temperature display 114 are arranged on the front end surface of the first tank body 11, and a temperature adjusting button 1141 is arranged at the right end of the temperature display 114; a temperature sensor 115 is fixedly arranged on the inner wall of the first tank body 11, and the temperature sensor 115 is electrically connected with a temperature display 114; the upper end surface of the first tank 11 is provided with an installation groove 116, and the inner wall of the installation groove 116 is uniformly coated with an anti-slip sealing material 1161. Through the installation of the sealing plug 12 and the anti-skid sealing material 1161, the purpose of sealing the device is achieved, and the culture effect is prevented from being influenced by the interference of other external microorganisms; the temperature sensor 115 is connected with the temperature display 114, so that the aim of timely grasping the culture temperature in the device is fulfilled, a proper temperature is conveniently provided, and the culture quality is improved.
Referring to fig. 4, a culture medium feeding device for blueberry tissue culture, a plurality of through holes 211 are arranged at the lower end of a liquid conveying pipe 21, and the diameter of the through holes 211 is one sixth of the diameter of the liquid conveying pipe 21. Through the installation of through-hole 211 with water pump 22, can effectively promote the liquid medium rate of addition of device to the work efficiency of device has been improved.
Referring to fig. 5, a culture medium adding device for blueberry tissue culture, wherein protective layers 4 are respectively installed on the inner walls of a first tank 11 and a second tank 32, each protective layer 4 is composed of a heat-insulating layer 41, a reinforcing layer 42 and a waterproof sealing layer 43, and the reinforcing layer 42 is arranged in an interlayer between the heat-insulating layer 41 and the waterproof sealing layer 43. Through the installation of protective layer 4, promoted and added the inside intensity of device, heat preservation thermal-insulated and waterproof sealing performance, more can adapt to complicated interpolation environment, prolonged the life of device, the cost is reduced.
In summary, the following steps: according to the culture medium adding device for blueberry tissue culture, a refrigerator 111 and a heater 112 are respectively arranged in an inner cavity of a first tank body 11, the rear end of the refrigerator 111 is electrically connected with a driving motor 1111, and the heater 112 is fixedly arranged at the right end of the inner wall of the first tank body 11; a sealing plug 12 is arranged at the upper end of the first tank body 11, a liquid outlet cylinder 13 is arranged at the upper end of the sealing plug 12, and the right side of the upper end surface of the liquid outlet cylinder 13 is connected with a transfusion catheter 2; the water pump 22 is arranged on the outer wall of the infusion tube 21, the lower end of the infusion tube 21 is provided with a plurality of through holes 211, and the liquid culture medium adding speed of the device can be effectively increased by arranging the through holes 211 and the water pump 22, so that the working efficiency of the device is improved; the right end of the transfusion tube 21 is communicated with the inner cavity of the culture tank 3, and the rubber plug 31 is fixedly arranged at the upper end of the second tank body 32; the inner wall of the first tank 11 is fixedly provided with a temperature sensor 115, the temperature sensor 115 is electrically connected with a temperature display 114, and the temperature sensor 115 is connected with the temperature display 114, so that the aim of timely grasping the culture temperature in the device is fulfilled, a proper temperature is conveniently provided, and the culture quality is improved; the upper end surface of the first tank body 11 is provided with an installation groove 116, the inner wall of the installation groove 116 is uniformly coated with the anti-skid sealing material 1161, and the anti-skid sealing material 1161 is installed, so that the purpose of a sealing device is achieved, and the culture effect is prevented from being influenced by interference of other external microorganisms; protective layer 4 is all installed to the inner wall of the first jar of body 11 and the second jar of body 32, and the installation of protective layer 4 has promoted and has added the inside intensity of device, heat preservation thermal-insulated and waterproof sealing performance, more can adapt to complicated interpolation environment, has prolonged the life of device, the cost is reduced.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (6)

1. The utility model provides a culture medium interpolation device for blueberry tissue culture, is connected with infusion pipe (2) including depositing fluid reservoir (1), infusion pipe (2) and culture tank (3) the up end of depositing fluid reservoir (1), and the right lower extreme of infusion pipe (2) is connected with culture tank (3), its characterized in that: the liquid storage tank (1) comprises a first tank body (11), a sealing plug (12) and a liquid outlet cylinder (13), wherein a refrigerator (111) and a heater (112) are respectively arranged in an inner cavity of the first tank body (11), the rear end of the refrigerator (111) is electrically connected with a driving motor (1111), and the heater (112) is fixedly arranged at the right end of the inner wall of the first tank body (11); a sealing plug (12) is arranged at the upper end of the first tank body (11), a liquid outlet cylinder (13) is arranged at the upper end of the sealing plug (12), and a transfusion catheter (2) is connected to the right side of the upper end face of the liquid outlet cylinder (13);
the infusion catheter (2) comprises an infusion tube (21) and a water pump (22), the water pump (22) is arranged on the outer wall of the infusion tube (21), and the right end of the infusion tube (21) is communicated with the inner cavity of the culture tank (3);
cultivate jar (3) including rubber buffer (31) and the second jar body (32), rubber buffer (31) fixed mounting is in the upper end of the second jar body (32).
2. The culture medium adding device for blueberry tissue culture as claimed in claim 1, wherein: an observation porthole (113) and a temperature display (114) are arranged on the front end face of the first tank body (11), and a temperature adjusting button (1141) is installed at the right end of the temperature display (114).
3. The culture medium adding device for blueberry tissue culture as claimed in claim 2, wherein: the inner wall of the first tank body (11) is fixedly provided with a temperature sensor (115), and the temperature sensor (115) is electrically connected with a temperature display (114).
4. The culture medium adding device for blueberry tissue culture as claimed in claim 1, wherein: the upper end surface of the first tank body (11) is provided with an installation groove (116), and the inner wall of the installation groove (116) is uniformly coated with anti-skid sealing materials (1161).
5. The culture medium adding device for blueberry tissue culture as claimed in claim 1, wherein: the lower end of the infusion tube (21) is provided with a plurality of through holes (211), and the diameter of each through hole (211) is one sixth of the diameter of the infusion tube (21).
6. The culture medium adding device for blueberry tissue culture as claimed in claim 1, wherein: the inner walls of the first tank body (11) and the second tank body (32) are respectively provided with a protective layer (4), each protective layer (4) consists of a heat-insulating layer (41), a reinforcing layer (42) and a waterproof sealing layer (43), and the reinforcing layer (42) is arranged in an interlayer between the heat-insulating layer (41) and the waterproof sealing layer (43).
CN201921737847.2U 2019-10-16 2019-10-16 A culture medium adds device for blueberry tissue culture Expired - Fee Related CN210841049U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201921737847.2U CN210841049U (en) 2019-10-16 2019-10-16 A culture medium adds device for blueberry tissue culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201921737847.2U CN210841049U (en) 2019-10-16 2019-10-16 A culture medium adds device for blueberry tissue culture

Publications (1)

Publication Number Publication Date
CN210841049U true CN210841049U (en) 2020-06-26

Family

ID=71299125

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201921737847.2U Expired - Fee Related CN210841049U (en) 2019-10-16 2019-10-16 A culture medium adds device for blueberry tissue culture

Country Status (1)

Country Link
CN (1) CN210841049U (en)

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CF01 Termination of patent right due to non-payment of annual fee
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Granted publication date: 20200626

Termination date: 20211016