CN210775230U - Dry chemical reagent strip capable of simultaneously and quantitatively detecting breast milk multi-parameter - Google Patents

Dry chemical reagent strip capable of simultaneously and quantitatively detecting breast milk multi-parameter Download PDF

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CN210775230U
CN210775230U CN201921421883.8U CN201921421883U CN210775230U CN 210775230 U CN210775230 U CN 210775230U CN 201921421883 U CN201921421883 U CN 201921421883U CN 210775230 U CN210775230 U CN 210775230U
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layer
reaction
breast milk
adhesive tape
reagent strip
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王维金
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Anhui Jiulu Biotechnology Co ltd
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Anhui Jiulu Biotechnology Co ltd
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Abstract

The utility model discloses a while quantitative determination breast milk multi-parameter's dry chemical reagent strip, including relative last supporting layer and the bottom suspension fagging that sets up, the surface equidistance complex of bottom suspension fagging has a plurality of reaction layers, has the diffusion layer at the surface complex of a plurality of reaction layers simultaneously to it has hydrophilic layer to compound between diffusion layer and last supporting layer. The utility model discloses a set up a plurality of reaction layers to different detection project load different reaction reagent in the reaction layer, can realize the detection of different materials on the reagent strip through once after the dropwise add sample, and then can be used for detecting the detection of protein, calcium ion, magnesium ion, zinc ion, iron ion and inorganic phosphorus in the breast milk simultaneously, and because do not meet between the reaction layer, cross contamination can not appear between each detection material, not only make the test result accurate, and it is convenient to test.

Description

Dry chemical reagent strip capable of simultaneously and quantitatively detecting breast milk multi-parameter
Technical Field
The utility model belongs to parameter measurement field in breast milk relates to a dry chemical reagent strip of while quantitative determination breast milk multi-parameter.
Background
The breast milk is a natural nutriment for babies, and the components of the breast milk directly influence the growth, development, intelligence development and digestive ability of the babies, so the breast milk cannot be replaced by the formula milk. The concentrations of protein, calcium ions, magnesium ions, zinc ions, iron ions and inorganic phosphorus in the milk have important significance for the nutritional value of the breast milk and the intake of babies, and the user can be reminded to adjust the self food structure and supplement the nutritional ingredients through the detection of the nutritional ingredients of the milk, so that the health of the breast is promoted, the milk quantity is increased, the milk ingredients are improved, substances harmful to the babies are prevented from being produced in the milk, and the breast milk is promoted to better meet the requirement of the normal development of the babies.
Protein, calcium ion, magnesium ion, zinc ion, iron ion and inorganic phosphorus detection methods are numerous. The measurement methods include ultrasonic infrared spectroscopy, liquid biochemical reagent method and dry chemical reagent method.
Compared with the ultrasonic infrared spectrometry and the liquid biochemical reagent method, the dry chemical reagent strip has the characteristics of low cost, small required sample amount, short detection time, simple, convenient and flexible operation, small pollution and the like.
At present, dry chemical reagent strips for detecting protein, calcium ions, magnesium ions, zinc ions, iron ions and inorganic phosphorus are mostly foreign products (such as Qiangsheng, Aikolai, Fuji and Roche), and have the problems of complex production process, high requirements on materials and equipment, easy generation of steam drums at a sample introduction end, excessive liquid, single detection item and the like. In addition, chinese patent CN201410532472.1 discloses a method for preparing a multi-parameter rapid detection card for dairy products, wherein the method requires pretreatment of a dairy product sample during inspection, and the detection card can be immersed in the sample after treatment and then taken out to be compared with a colorimetric card, thus the problems of serious cross contamination, large amount of required samples, complex operation and inaccurate detection result exist. Chinese patent CN98109147.4 discloses a reagent strip for visually observing calcium in breast milk, chinese patent CN20120130424.0 discloses a dry test strip for calcium determination and chinese patent CN298115107.8 discloses a dry analysis unit for protein detection, and the methods in these patents are all to qualitatively or quantitatively detect one item alone, and cannot detect multiple items simultaneously. At present, no dry chemical reagent strip capable of being used for detecting protein, calcium ions, magnesium ions, zinc ions, iron ions and inorganic phosphorus in breast milk exists.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a while quantitative determination breast milk multi-parameter's dry chemical reagent strip, through setting up a plurality of reaction layers, and the different reagents of different detection project loads in the reaction layer, can realize the detection of different materials on the reagent strip through once after the dropwise add sample, and then can be used for detecting protein in the breast milk simultaneously, calcium ion, magnesium ion, zinc ion, iron ion and inorganic phosphorus's detection, and because do not meet between the reaction layer, cross contamination can not appear between each detected material, not only make the test result accurate, and it is convenient to test
The purpose of the utility model can be realized by the following technical scheme:
the utility model provides a while quantitative determination breast milk multi-parameter's dry chemical reagent strip, includes relative last supporting layer and the under bracing layer that sets up, the surperficial equidistance complex of under bracing layer has a plurality of reaction layers, has the diffusion layer at the surface complex of a plurality of reaction layers simultaneously to it has hydrophilic layer to compound between diffusion layer and last supporting layer.
Furthermore, the surface of the lower supporting layer is bonded with a double-sided adhesive tape, the length and the width of the lower supporting layer are the same as those of the double-sided adhesive tape, the surface of the lower supporting layer is provided with a plurality of testing holes which are arranged at equal intervals and are not communicated, each testing hole is circular and has the same inner diameter, and the surface of the double-sided adhesive tape is provided with a plurality of circular through holes corresponding to the testing holes.
Furthermore, the reaction layers correspond to the permeation holes in the surface of the double-sided adhesive tape one to one, the center of each reaction layer coincides with the circle center of the permeation hole, and the reaction layers are bonded at the permeation holes of the double-sided adhesive tape.
Furthermore, the width of the diffusion layer is 3-4mm larger than that of the reaction layer, the bottom surface of the diffusion layer is tightly pressed and connected with the surface of the reaction layer, and two sides of the diffusion layer extend out of two sides of the surface of the reaction layer and then are adhered to the surface of the double-sided adhesive tape.
Furthermore, the width of the hydrophilic layer is 3-4mm larger than that of the diffusion layer, meanwhile, the width of the hydrophilic layer is 4-5mm smaller than that of the double faced adhesive tape, the bottom surface of the hydrophilic layer is tightly pressed and connected with the surface of the diffusion layer, and the two sides of the hydrophilic layer extend out of the two sides of the surface of the diffusion layer and then are bonded on the surface of the double faced adhesive tape.
Furthermore, the width of the upper supporting layer is the same as that of the lower supporting layer, the middle part of the surface of the upper supporting layer is provided with a sample inlet hole, and the two sides of the upper supporting layer extend out of the surface of the hydrophilic layer and then are bonded on the surface of the double-sided adhesive tape.
Furthermore, the upper support layer and the lower support layer are both made of high molecular polymer materials, wherein the high molecular polymer materials are one of polycarbonate, polystyrene, polyvinyl chloride or polyethylene.
Furthermore, the hydrophilic layer is made of polyester material, and one of polyvinylpyrrolidone, polyethylene glycol or polyvinyl alcohol is impregnated and loaded on the surface of the polyester material.
Furthermore, the diffusion layer is made of one or more of porous nylon, polypropylene, polyester fiber, cotton fabric or mesh fabric.
Further, the reaction layer is a polysulfone membrane or a polyethersulfone membrane.
The utility model has the advantages that:
1. the utility model discloses a set up a plurality of reaction layers to the different realization of the test reagent of load is different in the reaction layer, can realize the detection of different materials on the reagent strip through once after the dropwise add sample, and then can be used for detecting the detection of protein, calcium ion, magnesium ion, zinc ion, iron ion and inorganic phosphorus in the breast milk simultaneously, and do not meet between the hesitation reaction layer, cross contamination can not appear between each detection material, not only make the test result accurate, and it is convenient to test.
2. The utility model discloses a dry chemical reagent strip can realize the detection to six detection items of calcium ion, magnesium ion, zinc ion, iron ion and inorganic phosphorus in the sample simultaneously to can be so that the testing result is more accurate, convenient operation, and reduce the test sample, reduce environmental pollution, shorten check-out time, the cost is reduced simultaneously, is suitable for to promote in the extensive family at home.
3. The utility model discloses a dry chemical reagent strip that membrane materials such as back-up layer, diffusion layer, filter layer, reaction layer and double faced adhesive tape made constitutes a siphon system through the coincide, has further guaranteed the speed of detection, the accuracy of result and can obtain a plurality of detection parameters simultaneously.
Drawings
In order to facilitate understanding for those skilled in the art, the present invention will be further described with reference to the accompanying drawings.
Fig. 1 is an exploded view of the structure of the dry chemical reagent strip of the present invention.
Detailed Description
Example 1:
a dry chemical reagent strip for simultaneously and quantitatively detecting breast milk multiparameters comprises an upper support layer 1 and a lower support layer 2 which are oppositely arranged, wherein a plurality of reaction layers 5 are compounded on the surface of the lower support layer 2 at equal intervals, diffusion layers 4 are compounded on the surfaces of the reaction layers 5, and hydrophilic layers 3 are compounded between the diffusion layers 4 and the upper support layer 1;
the surface of the lower supporting layer 2 is adhered with a double-sided adhesive tape 6, the length and the width of the lower supporting layer 2 are the same as those of the double-sided adhesive tape 6, the surface of the lower supporting layer 2 is provided with a plurality of test holes 21 which are arranged at equal intervals and are not communicated, each test hole 21 is circular, has the same inner diameter and is 4mm, and the surface of the double-sided adhesive tape 6 is provided with a plurality of circular through holes 61 corresponding to the test holes 21; the change conditions of the reaction color and the reflectivity on each reaction layer 5 are convenient to observe and measure through the test holes 21 and the circular transmission holes 61;
the reaction layers 5 correspond to the penetration holes 61 on the surface of the double-sided adhesive tape 6 one by one, meanwhile, the center of each reaction layer 5 is superposed with the circle center of the penetration hole 61, and the reaction layers 5 are directly adhered to the penetration holes 61 of the double-sided adhesive tape 6;
the width of the diffusion layer 4 is 3-4mm larger than that of the reaction layer 5, the bottom surface of the diffusion layer 4 is tightly pressed and connected with the surface of the reaction layer 5, meanwhile, because the width of the diffusion layer 4 is larger than that of the reaction layer 5, the two sides of the diffusion layer 4 extend out of the two sides of the surface of the reaction layer 5 and then are adhered to the surface of the double-sided adhesive tape 6, and the diffusion layer 4 is fixed through the double-sided adhesive tape 6;
the width of the hydrophilic layer 3 is 3-4mm larger than that of the diffusion layer 4, meanwhile, the width of the hydrophilic layer 3 is 4-5mm smaller than that of the double faced adhesive tape 6, the bottom surface of the hydrophilic layer 3 is tightly pressed and connected with the surface of the diffusion layer 4, meanwhile, because the width of the hydrophilic layer 3 is larger than that of the diffusion layer 4, and the width of the hydrophilic layer 3 is smaller than that of the double faced adhesive tape 6, the two side sides of the hydrophilic layer 3 extend out of the two side sides of the surface of the diffusion layer 4 and then are adhered to the surface of the double faced adhesive tape 6, and the hydrophilic layer 3 is fixed through the double faced adhesive tape;
the width of the upper supporting layer 1 and the thickness of the lower supporting layer 2 are the same and 0.4mm, the size of the upper supporting layer 1 and the size of the lower supporting layer 2 are both 48 x 6mm, meanwhile, the middle part of the surface of the upper supporting layer 1 is provided with a sampling hole 11, wherein the sampling hole 11 is rectangular and has the size of 4 x 5mm, and the sampling hole 11 is used for dropwise adding a sample; because the width of the upper support layer 1 is larger than that of the hydrophilic layer 3 and is equal to that of the double-sided adhesive tape 6, the two sides of the upper support layer 1 extend out of the surface of the hydrophilic layer 3 and then are bonded on the surface of the double-sided adhesive tape 6, and the upper support layer 1 is fixed through the double-sided adhesive tape 6; therefore, the reaction layer 5, the diffusion layer 4, the hydrophilic layer 3, the upper support layer 1 and the lower support layer 2 are fixed through the double faced adhesive tape 6 by different widths, so that not only is the fixing material saved, but also the blocking among the reaction layer 5, the diffusion layer 4 and the hydrophilic layer 3 is reduced, the rapid permeation of detection liquid is facilitated, and the upper support layer 1 and the lower support layer 2 can be recycled by directly tearing off the side from the double faced adhesive tape 6 after the use is finished, so that the use is convenient and the recovery is convenient;
the lengths of the upper support layer 1, the lower support layer 2, the hydrophilic layer 3 and the diffusion layer 4 are the same, so that the sides of the prepared reagent strip are neat, and the phenomenon that when one layer is short, detection liquid cannot be fully contacted with the next layer from the side of the upper layer to influence the permeability is prevented;
the specific application method of the dry chemical reagent strip is as follows:
firstly, dripping a sample on a reagent strip through a sample inlet hole 11;
and secondly, the sample directly reaches the surface of the hydrophilic layer 3 after passing through the sample inlet 11, uniformly and rapidly diffuses leftwards and rightwards through the hydrophilic layer 3, downwards diffuses to the diffusion layer 4, then reaches the reaction layer 5 to react with the reagent, and finally, the test holes of all the test items show different color changes to realize quantitative detection according to the different concentrations of the substances to be detected in the sample.
Example 2:
the upper supporting layer 1 and the lower supporting layer 2 are both made of high molecular polymer materials, wherein the high molecular polymer materials are one of polycarbonate, polystyrene, polyvinyl chloride or polyethylene, and the upper supporting layer 1 and the lower supporting layer 2 play a main supporting role;
the hydrophilic layer 3 is made of polyester materials, one of polyvinylpyrrolidone, polyethylene glycol or polyvinyl alcohol is impregnated and loaded on the surface of the polyester materials, a plurality of diffusion holes are uniformly distributed on the surface of the hydrophilic layer 3, the pore size of each diffusion hole is 10-500 microns, the thickness of each diffusion hole is 1-20 microns, and the purpose of the method is to enable a sample to be rapidly diffused;
the diffusion layer 4 is made of one or more of porous nylon, polypropylene, polyester fiber, cotton fabric or mesh cloth, the pore size of the diffusion layer is 20-200um, the thickness of the diffusion layer is 0.1-0.4mm, in order to enable a sample to be uniformly and rapidly diffused to the reaction layer, the material of the diffusion layer 4 is soaked by a surfactant and then is subjected to hydrophilic treatment, wherein the surfactant is a nonionic surfactant, such as polyethylene glycol octyl phenyl ether or polysorbate, and the diffusion layer can be treated by adopting a soaking mode;
the reaction layer 5 is a polysulfone membrane or a polyether sulfone membrane, wherein the pore size of the membrane is 0.4-7um, and the thickness of the membrane is 80-20 um; meanwhile, before the reagent strip is prepared, different test reagents are soaked in the reaction layer 5, and after the test reagents are dried, the reaction layer 5 is bonded, wherein the test reagents correspond to different test reagents according to protein, calcium ions, magnesium ions, zinc ions, iron ions and inorganic phosphorus to be detected in a sample, so that six different test reagents capable of respectively detecting the protein, the calcium ions, the magnesium ions, the zinc ions, the iron ions and the inorganic phosphorus are loaded in 6 prepared reaction layers 5.
Example 3:
the reaction reagent for detecting protein comprises 15-30g of oxalic acid, 0.5-2g of potassium oxalate, 0.5-2g of ammonium molybdate, 0.01-1g of pyrogallol red, 0.01-0.1g of triton, 0.01-0.5g of tween, 0.5-1g of sodium hydroxymethyl cellulose and 50-100ml of purified water or distilled water.
The reaction reagent for detecting calcium ions comprises 10-20g of pyrophosphoric acid, 0.04-0.1g of azoarsine-III, 3-10ml of ethanol, 0.2-1g of trehalose, 0.1-0.5g of 8-hydroxyquinoline, 0.05-0.2g of triton, 0.2-1g of cyclodextrin, 0.1-1ml of hydrochloric acid and 100ml of purified water or distilled water.
The reaction reagent for detecting magnesium ions comprises 5-10g of boric acid, 0.1-2g of xylidine blue, 1-5g of cane sugar, 1-5ml of methanol, 0.1-0.5g of oxalic acid tetraacetic acid, 0.05-0.2g of triton, 0.2-1g of polyethylene glycol, 0.01-0.5g of sodium hydroxide and 60-120ml of purified water or distilled water.
The reaction reagent for detecting zinc ions comprises 10-30g of 2- (N-morpholine) ethanesulfonic acid, 0.1-2g of 4- (2-pyridylazo) resorcinol sodium salt, 5-10ml of dimethyl sulfoxide, 0.5-2g of sucrose, 0.2-0.6g of thiourea, 0.1-1g of sodium citrate, 0.5-5g of acrylamide, 0.01-1g of sodium hydroxide and 100ml of purified water or distilled water.
The reaction reagent for detecting ferric ions comprises 5-10g of glycine, 0.05-1g of ferrocyanide, 3-10ml of ethanol, 0.05-0.4g of ascorbic acid, 0.01-0.1g of potassium ferrocyanide, 0.1-1g of sodium citrate, 0.1-2g of ethylene glycol, 0.5-10ml of hydrochloric acid and 100-200ml of purified water or distilled water.
The reaction reagent for detecting inorganic phosphorus comprises 15-40g of citric acid, 0.01-0.5g of malachite green, 1-5ml of ethanol, 0.5-2g of trehalose, 0.01-0.3g of sodium citrate, 0.05-1g of bovine serum albumin, 0.3-2g of urea, 1-8ml of hydrochloric acid and 50-120ml of purified water or distilled water.
The preferred embodiments of the present invention disclosed above are intended only to help illustrate the present invention. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and its practical applications, to thereby enable others skilled in the art to best understand the invention for and utilize the invention. The present invention is limited only by the claims and their full scope and equivalents.

Claims (8)

1. The utility model provides a while quantitative determination breast milk multi-parameter's dry chemical reagent strip, its characterized in that, including relative last supporting layer (1) and lower supporting layer (2) that set up, the surface equidistance complex of lower supporting layer (2) has a plurality of reaction layers (5), has diffusion layer (4) at the surface complex of a plurality of reaction layers (5) simultaneously to it has hydrophilic layer (3) to compound between diffusion layer (4) and last supporting layer (1).
2. The dry chemical reagent strip for simultaneously and quantitatively detecting multiple parameters of breast milk according to claim 1, wherein a double-sided adhesive tape (6) is bonded on the surface of the lower support layer (2), the length and the width of the lower support layer (2) are the same as those of the double-sided adhesive tape (6), a plurality of test holes (21) which are equidistantly arranged and are not communicated are formed in the surface of the lower support layer (2), each test hole (21) is circular and has the same inner diameter, and a plurality of circular through holes (61) corresponding to the test holes (21) are formed in the surface of the double-sided adhesive tape (6).
3. The dry chemical reagent strip for simultaneously and quantitatively detecting multiple parameters of breast milk according to claim 1, wherein a plurality of reaction layers (5) correspond to a plurality of through holes (61) on the surface of the double-sided adhesive tape (6) one by one, the center of each reaction layer (5) coincides with the circle center of each through hole (61), and the reaction layers (5) are adhered to the through holes (61) of the double-sided adhesive tape (6).
4. The dry chemical reagent strip for simultaneously and quantitatively detecting multiple parameters of breast milk according to claim 1, wherein the width of the diffusion layer (4) is 3-4mm larger than that of the reaction layer (5), the bottom surface of the diffusion layer (4) is in pressed connection with the surface of the reaction layer (5), and the two sides of the diffusion layer (4) extend out of the two sides of the surface of the reaction layer (5) and then are adhered to the surface of the double-sided adhesive tape (6).
5. The dry chemical reagent strip for simultaneously and quantitatively detecting multiple parameters of breast milk according to claim 1, wherein the width of the hydrophilic layer (3) is 3-4mm larger than that of the diffusion layer (4), the width of the hydrophilic layer (3) is 4-5mm smaller than that of the double-sided adhesive tape (6), the bottom surface of the hydrophilic layer (3) is tightly pressed and connected with the surface of the diffusion layer (4), and the two side sides of the hydrophilic layer (3) extend out of the two side sides of the surface of the diffusion layer (4) and then are adhered to the surface of the double-sided adhesive tape (6).
6. The dry chemical reagent strip for simultaneously and quantitatively detecting multiple parameters of breast milk according to claim 1, wherein the width of the upper support layer (1) is the same as that of the lower support layer (2), the middle part of the surface of the upper support layer (1) is provided with a sample inlet hole (11), and the two sides of the upper support layer (1) extend out of the surface of the hydrophilic layer (3) and then are adhered to the surface of the double-sided adhesive tape (6).
7. The dry chemical reagent strip for simultaneously and quantitatively detecting multiple parameters of breast milk according to claim 1, wherein the hydrophilic layer (3) is made of polyester material.
8. The dry chemical reagent strip for simultaneously and quantitatively detecting multiple parameters of breast milk according to claim 1, wherein the reaction layer (5) is a polysulfone membrane or a polyethersulfone membrane.
CN201921421883.8U 2019-08-29 2019-08-29 Dry chemical reagent strip capable of simultaneously and quantitatively detecting breast milk multi-parameter Active CN210775230U (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110412027A (en) * 2019-08-29 2019-11-05 安徽九陆生物科技有限公司 A kind of dry chemistry reagent item of simultaneous quantitative detection breast milk multi-parameter

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110412027A (en) * 2019-08-29 2019-11-05 安徽九陆生物科技有限公司 A kind of dry chemistry reagent item of simultaneous quantitative detection breast milk multi-parameter

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