CN210711584U

CN210711584U - Dental pulp stem cell culture apparatus

Info

Publication number: CN210711584U
Application number: CN201921163186.7U
Authority: CN
Inventors: 王雨; 梅志超; 余涛; 罗新高
Original assignee: Shenzhen Qianhai Precision Bio Tech Co ltd
Current assignee: Shenzhen Kekang Clinical Medicine Research Co.,Ltd.
Priority date: 2019-07-23
Filing date: 2019-07-23
Publication date: 2020-06-09
Anticipated expiration: 2029-07-23

Abstract

The utility model discloses a dental pulp stem cell culture device, which belongs to the technical field of cell culture and comprises a device main body, wherein a groove is arranged in the middle of the bottom of the device main body, a bottom plate is arranged below the device main body, a lug extending to the inside of the groove is connected in the middle of the top of the bottom plate, the dental pulp stem cell culture device is provided with a dropping bottle, an air bag and a dropping liquid head, when a user uses the culture device to culture dental pulp stem cells, nutrient solution can be firstly added into the dropping bottle through a liquid injection pipe, so that the user can extrude the air bag when the user needs to add nutrient solution into the cell culture solution in the cell culture process, the air bag pushes the nutrient solution in the dropping bottle to drop into the device main body through the dropping liquid head after being extruded, thereby avoiding the situation that the user needs to open a cover plate to add nutrient solution, thereby reducing the possibility of pollution to the cell culture, thereby greatly improving the culture effect of the device.

Description

Dental pulp stem cell culture apparatus

Technical Field

The utility model relates to a cell culture technical field specifically is a dental pulp stem cell culture apparatus.

Background

The dental pulp stem cell is the only soft tissue in the dental tissue, and the research on the dental pulp cell by Gronthos in 2000 discovers that the dental pulp stem cell has immunophenotype and the capability of forming mineralized nodules which are very similar to the mesenchymal stem cell in the marrow, and the shape of the cell is fusiform, so that the dental pulp stem cell can be self-renewed and multi-directionally differentiated, and has stronger cloning capability. These fibroblasts isolated from dental pulp tissue are called dental pulp stem cells.

But current dental pulp stem cell culture device, when the user carries out cell culture and uses, often need to annotate the nutrient solution to cell culture liquid, and current device is when annotating the nutrient solution, need open the apron, consequently very easily lead to the culture solution to receive the pollution, thereby influence the device to the culture effect of stem cell, and its bottom of current culture apparatus is comparatively level and smooth mostly, consequently the user is when placing the device main part on the desktop, very easily take place to slide, thereby cause the device to drop and damage.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a dental pulp stem cell culture device to when providing to open the apron and fill the nutrient solution in solving above-mentioned background art, very easily cause cell culture liquid to receive the pollution, and the device bottom is comparatively level and smooth, very easily takes place to slide, thereby causes the device to drop the problem of damage.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a dental pulp stem cell culture apparatus, includes the device main part, set up flutedly in the middle of the bottom of device main part, the below of device main part is provided with the bottom plate, the top intermediate junction of bottom plate has the lug that extends to the recess is inside, the sucking disc is all installed to the bottom both sides of bottom plate, the apron is installed through the mounting groove in the top of device main part, the top intermediate junction of apron has the dropper, the gasbag is installed at the top of dropper, the bottom of dropper is connected with the dripper that extends to the inside of device main part.

Preferably, one side of the dropping bottle is connected with a liquid injection pipe, and one end of the liquid injection pipe is provided with a sealing plug.

Preferably, threads are distributed on the inner wall of the mounting groove and the lower portions of the two sides of the cover plate at equal intervals, and the mounting groove is meshed with the cover plate through the threads.

Preferably, the two suckers are made of butadiene-acrylonitrile rubber and are symmetrically arranged along the center line of the longitudinal axis of the bottom plate.

Preferably, a plurality of pits are arranged below the inner part of the device main body, and the height of the plurality of pits gradually decreases along the directions of two sides of the device main body.

Preferably, the dropping bottle is spherical, and the manufacturing material of the dropping bottle comprises polyethylene.

Compared with the prior art, the beneficial effects of the utility model are that: the dental pulp stem cell culture device is provided with a dropping bottle, an air bag and a dropping liquid head, when a user uses the culture device to culture dental pulp stem cells, nutrient solution can be filled into the dropping bottle through a liquid injection pipe firstly, so that when the user needs to add nutrient solution into the cell culture solution in the cell culture process, the user can squeeze the air bag to push the nutrient solution in the dropping bottle to be dripped into the device main body through the dropping liquid head after the air bag is squeezed, the situation that the user needs to open a cover plate to fill the nutrient solution is avoided, the possibility that the cell culture is polluted is reduced, the culture effect of the device is greatly improved, meanwhile, the device is also provided with a bottom plate, a sucking disc, a lug and a groove, when the user places the device main body, the bottom plate can be adsorbed and fixed on a desktop through the sucking disc firstly, then the device main body is placed on the bottom plate, and the lug at the top of the bottom plate is inserted into the groove in the middle of the bottom of the device, with this realization to the device main part spacing, avoid the device main part to take place to slide and fall and damage, and when the user need take the removal to the device main part, only need with the device main part upwards take up can, make the user operation more simple and convenient.

Drawings

FIG. 1 is a schematic structural view of the present invention;

fig. 2 is an enlarged schematic view of a partial structure of the present invention a;

fig. 3 is an enlarged schematic view of a local structure of the present invention B.

In the figure: 1. a device main body; 2. a base plate; 3. a suction cup; 4. a bump; 5. a groove; 6. mounting grooves; 7. a cover plate; 8. dropping a bottle; 9. an air bag; 10. a liquid injection pipe; 11. a sealing plug; 12. a liquid dropping head; 13. and (4) pits.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the present invention, unless otherwise expressly stated or limited, the terms "disposed," "mounted," "connected," "fixed," "sleeved," and the like are to be construed broadly, e.g., as either a fixed connection, a removable connection, or an integral part; can be mechanically or electrically connected; the two elements may be connected directly or indirectly through an intermediate medium, and the two elements may be connected internally or in an interaction relationship, and a person skilled in the art can understand the specific meaning of the above terms in the present invention according to specific situations.

Referring to fig. 1-3, the present invention provides a technical solution: the utility model provides a dental pulp stem cell culture apparatus, includes device main part 1, bottom plate 2, sucking disc 3, lug 4, recess 5, mounting groove 6, apron 7, dropper 8, gasbag 9, annotates liquid pipe 10, sealing plug 11, dripper 12 and pit 13, set up fluted 5 in the middle of the bottom of device main part 1, the below of device main part 1 is provided with bottom plate 2, the top intermediate junction of bottom plate 2 extends to the inside lug 4 of recess 5, sucking disc 3 is all installed to the bottom both sides of bottom plate 2, apron 7 is installed through mounting groove 6 at the top of device main part 1, the top intermediate junction of apron 7 has dropper 8, gasbag 9 is installed at the top of dropper 8, the bottom of dropper 8 is connected with and extends to the inside dripper 12 of device main part 1, and the dripper 12 of being convenient for instils nutrient solution into inside the device main part with the nutrient solution.

Referring to fig. 1 and 2, one side of the drip bottle 8 is connected with an injection tube 10, one end of the injection tube 10 is provided with a sealing plug 11, so that a user can conveniently inject nutrient solution into the drip bottle 8 through the injection tube 10, the sealing plug 11 can help to seal the injection tube 10, so that impurities are prevented from entering the drip bottle 8 through the injection tube 10, threads are distributed on the inner wall of the mounting groove 6 and below the two sides of the cover plate 7 at equal intervals, the mounting groove 6 is meshed with the cover plate 7 through the threads, and the user can conveniently install the cover plate 7 on the top of the device main body 1 through the mounting groove 6.

Referring to fig. 1 and 3, the two suckers 3 are made of nitrile rubber, the two suckers 3 are symmetrically arranged along a longitudinal axis center line of the base plate 2, so that when a user places the base plate 2, the suckers 3 can be adsorbed on a table top to fix the base plate 2, a plurality of pits 13 are formed in the lower portion of the inside of the device body 1, and the heights of the plurality of pits 13 gradually decrease along directions of two sides of the device body 1, so that stem cells can be cultured and grown in the pits 13.

Referring to fig. 1, the dropper bottle 8 is spherical, and the material for manufacturing the dropper bottle 8 includes polyethylene, which facilitates increasing the volume of the dropper bottle 8, and the polyethylene increases the service life of the dropper bottle 8.

The working principle is as follows: firstly, when a user uses the device, the bottom plate 2 can be fixed on a table surface through the suction disc 3, then the device main body 1 is placed on the bottom plate 2, the convex block 4 at the top of the bottom plate 2 is inserted into the groove 5 in the middle of the bottom of the device main body 1, so that the device main body 1 is limited, when the device main body 1 is placed, the user can put cells and culture solution into the device main body 1, when the cells and the culture solution are placed, the user can insert the cover plates 7 into the mounting grooves 6 arranged at the two sides of the top of the device main body 1 and rotate the cover plates 7, so that the cover plates 7 are fixed at the top of the device main body 1, when the cover plates 7 are fixed, the user can open the sealing plugs 11, the nutrient solution is filled into the dropping bottles 8 through the liquid filling pipes 10, when the nutrient solution is filled, the user can close the sealing plugs 11, and when the cells are cultured, when nutrient solution needs to be added, a user can squeeze the air bag 9, so that the air bag 9 pushes the nutrient solution in the dropping bottle 8 to drop into the device main body 1 through the dropping head 12 after being squeezed.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A dental pulp stem cell culture apparatus includes an apparatus main body (1), characterized in that: offer recess (5) in the middle of the bottom of device main part (1), the below of device main part (1) is provided with bottom plate (2), the top intermediate junction of bottom plate (2) has lug (4) that extend to recess (5) inside, sucking disc (3) are all installed to the bottom both sides of bottom plate (2), apron (7) are installed through mounting groove (6) in the top of device main part (1), the top intermediate junction of apron (7) has dropper (8), gasbag (9) are installed at the top of dropper (8), the bottom of dropper (8) is connected with the dripper (12) that extend to device main part (1) inside.

2. The dental pulp stem cell culture apparatus according to claim 1, wherein: one side of the dropping bottle (8) is connected with a liquid injection pipe (10), and one end of the liquid injection pipe (10) is provided with a sealing plug (11).

3. The dental pulp stem cell culture apparatus according to claim 1, wherein: threads are distributed on the inner wall of the mounting groove (6) and the lower portions of the two sides of the cover plate (7) at equal intervals, and the mounting groove (6) is meshed with the cover plate (7) through the threads.

4. The dental pulp stem cell culture apparatus according to claim 1, wherein: the two suckers (3) are made of butadiene-acrylonitrile rubber, and the two suckers (3) are symmetrically arranged along the center line of the longitudinal axis of the bottom plate (2).

5. The dental pulp stem cell culture apparatus according to claim 1, wherein: a plurality of pits (13) are arranged below the inner part of the device main body (1), and the heights of the pits (13) gradually decrease along the directions of two sides of the device main body (1).

6. The dental pulp stem cell culture apparatus according to claim 1, wherein: the dropping bottle (8) is spherical, and the manufacturing material of the dropping bottle (8) comprises polyethylene.