CN210690417U - Detection apparatus for abrus cantoniensis hance in animal liver injury experiment - Google Patents
Detection apparatus for abrus cantoniensis hance in animal liver injury experiment Download PDFInfo
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- CN210690417U CN210690417U CN201921373858.7U CN201921373858U CN210690417U CN 210690417 U CN210690417 U CN 210690417U CN 201921373858 U CN201921373858 U CN 201921373858U CN 210690417 U CN210690417 U CN 210690417U
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Abstract
The utility model discloses a detection device of abrus cantoniensis hance in animal liver injury experiments, which comprises a shell, wherein a serum placing device and a serum detection device are rotationally connected in the shell, the serum placing device comprises a serum placing disc and a first rotating shaft, the serum placing disc is provided with a plurality of test tube placing holes, each test tube placing hole is provided with a test tube placing frame, each test tube placing frame is upwards provided with a hollow stabbing needle, and a test tube is placed in each test tube placing frame; the serum detection device comprises a reaction card placing disc and a second rotating shaft, wherein the reaction card placing disc is provided with a plurality of reaction card placing grooves, and a reagent reaction card is placed in each reaction card placing groove; the first rotating shaft and the second rotating shaft are sleeved with a driving belt, the upper end of the shell is provided with a through hole, and a pressure pipe device is inserted into the through hole. The utility model discloses can carry out batch detection to the experiment serum that awaits measuring in the abrus cantoniensis is to animal liver damage experiment, convenient operation is swift, can effectively improve detection quality simultaneously.
Description
Technical Field
The utility model relates to a medical science experiment apparatus technical field, in particular to detection device of abrus cantoniensis hance in animal liver injury experiment.
Background
Abrus herb, also known as cantonese abrus. Experimental research shows that: the herba abri contains triterpenoid saponins, triterpenes, anthraquinones, alkaloids, etc. It has pharmacological effects of protecting liver, resisting inflammation, and promoting immunity. Carbon tetrachloride is commonly used for duplicating a liver injury animal model in an animal liver injury experiment, the protection effect of the abrus herb alcohol extract on liver injury animals is discussed, and scientific basis is provided for reasonably developing rich abrus herb medicinal materials.
Alanine Aminotransferase (ALT) and aspartate Aminotransferase (AST) are mainly distributed in cytoplasm, mitochondria and cytoplasm of liver respectively, when liver cells are damaged due to various reasons, the enzymes overflow from the inside and outside of the liver cells into blood, so that the concentrations of alanine Aminotransferase (ALT) and aspartate Aminotransferase (AST) in the blood are increased, and thus the alanine Aminotransferase (ALT) and the aspartate Aminotransferase (AST) are important indexes for reflecting the liver function.
In the protection experiment of studying abrus cantoniensis hance to animal liver damage, the blood of liver damage animal is usually extracted, the serum is centrifuged from the blood, the damage degree of liver cells is reflected by the activity of glutamic-pyruvic transaminase (ALT) and glutamic-oxalacetic transaminase (AST) in the detected serum, in order to ensure the accuracy of the experimental result, a plurality of experimental groups and a control group are usually selected in the experiment for result comparison, therefore, the quantity of the serum that the experimenter needs to detect is larger, a method of sequentially dripping the experimental serum for color development on a reagent reaction card and then carrying out manual data comparison is adopted, the detection efficiency of the experiment is lower, the serum is dripped onto a detection device, the error of the operator occurs under an unexpected condition, and the serum is dripped onto the reagent reaction card, thereby the detection quality is influenced, and the detection effect is poor.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a detection apparatus of abrus herb in animal liver damage experiment, can carry out batch detection to the experiment serum that awaits measuring in abrus herb is to animal liver damage experiment, convenient operation is swift, can accurately drip reagent reaction card with serum simultaneously, improves detection quality.
The above technical purpose of the present invention can be achieved by the following technical solutions: the utility model provides a detection apparatus of abrus cantoniensis hance in animal liver injury experiment, includes the casing, the casing internal rotation is connected with serum placer and serum detection device, the serum placer includes serum place the dish, run through the serum place set center and with the first pivot of casing rotation connection, run through the serum place set and open and be equipped with a plurality of test tubes that are the circumference and distribute and place the hole, the lower extreme that each test tube placed the hole all is provided with test tube and places the frame, the bottom that runs through each test tube and sets up the frame is all upwards provided with the cavity and stabs the needle, the test tube of cavity both ends open-ended has all been placed in each test tube places the frame, the lower extreme of each test tube;
the serum detection device comprises a reaction card placing disc arranged below the test tube placing frame and a second rotating shaft which penetrates through the center of the reaction card placing disc and is rotatably connected with the shell, the reaction card placing disc is provided with a plurality of reaction card placing grooves which are distributed in a circumferential manner and correspond to the test tube placing holes one by one, and a reagent reaction card is placed in each reaction card placing groove;
first pivot with the overcoat of second pivot has the drive belt, the casing is close to serum placer's one end and is close to serum detection device's one end all is provided with the opening, the casing upper end is relative serum place the dish with the upper end of serum detection dish has seted up the through-hole, the through-hole interpolation is equipped with presses the pipe device, press the pipe device including inserting and locate depression bar in the through-hole, with the depression bar is close to the briquetting that the one end of serum place the dish is connected, with the depression bar is kept away from the spacing ring that the one end of serum place the dish is connected, the spacing ring with install the cover between the casing and locate the outer reset spring of depression bar, work as test tube placement frame rotates when the briquetting under, the reaction card standing groove rotates test tube placement frame under.
By adopting the technical scheme, when serum is detected, firstly, the separated serum is respectively loaded into the test tubes, the serum placing tray is manually rotated, each test tube is placed in the corresponding test tube placing frame, and the reagent reaction card is placed in the reaction card placing groove;
rotate the serum and place the dish afterwards, make one of them test tube be located the below of pressing the pipe device, the reaction card standing groove is located the below of test tube this moment, press and press the pipe device, press the pipe device and push down the test tube, make cavity stab the needle and pierce the brine bottle rubber buffer, the serum in the test tube punctures the needle through cavity and drips on the reagent reaction card, reagent reaction card takes place the colour transform with the serum reaction, repeat above-mentioned step, make the equal reagent reaction card reaction of serum in each test tube, accomplish the serum and detect.
The utility model discloses a further set up to: detection terminal is installed to the upper end of casing, the last display screen that is provided with of detection terminal, install the controller in the detection terminal, the inboard upper end of casing be provided with the corresponding detection device in groove position is placed to the reaction card, detection device's lower extreme is provided with the optical detection response piece, display screen, optical detection response piece all with the controller electricity links.
Through adopting above-mentioned technical scheme, after serum and reagent reaction card reaction, rotate the serum and place the dish, make reagent reaction card position under the optical detection response piece, make the optical detection response piece detect reagent reaction card through control button, the information transmission who detects gives the controller, the controller handles the information that detects the back and shows through the display screen, obtains the structure that the serum detected, repeats above-mentioned step, detects each reagent reaction card, accomplishes the serum and detects.
The utility model discloses a further set up to: the periphery of first pivot with second pivot lower extreme all sets up a plurality of draw-in grooves that are the circumference and distribute, the inboard of drive belt be provided with a plurality of with draw-in groove assorted card strip.
Through adopting above-mentioned technical scheme, rotate the serum and place the dish, the drive belt drives the serum and detects the dish and rotate, and the joint through draw-in groove and card strip makes the serum place the dish and place the rotation synchronization of dish and reaction card and can not appear the deviation.
The utility model discloses a further set up to: one end of each hollow poking needle close to the serum placing disc is in a needle tip inclined opening shape.
By adopting the technical scheme, one side of the detection terminal is provided with the power line electrically connected with the controller.
The utility model discloses a further set up to: and the detection terminal is provided with a plurality of control buttons electrically connected with the controller.
The utility model discloses a further set up to: and the detection terminal is provided with a plurality of control buttons electrically connected with the controller.
The utility model discloses a further set up to: the reagent reaction cards are alanine Aminotransferase (ALT) reagent reaction cards and aspartate Aminotransferase (AST) reagent reaction cards.
To sum up, the utility model discloses following beneficial effect has:
firstly, through the device, experimenters can carry out batch serum detection, a plurality of test tubes filled with experimental serum are placed into the device at one time, the detection serum does not need to be placed back and forth, the detection efficiency is improved, and meanwhile, the serum can be accurately dripped onto a reagent reaction card, and the detection quality is improved;
and secondly, the detection result is directly generated into data through the detection terminal, the detection result does not need to be judged manually, the detection accuracy is improved, and meanwhile, the judgment and recording time is saved.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is primarily used to show the detection device;
FIG. 3 is a view mainly showing the connection relationship between the serum placement device and the serum detection device;
FIG. 4 is a view showing the connection relationship between the test tube and the rubber stopper of the saline bottle;
fig. 5 is mainly used for showing the connection relationship between the pressure pipe device and the shell.
In the figure: 1. a housing; 2. a serum placement device; 21. a serum placing tray; 22. a first rotating shaft; 23. test tube placement holes; 24. a test tube placement frame; 25. a hollow poking needle; 3. a test tube; 31. a saline bottle rubber plug; 4. a serum detection device; 41. a reaction card placing tray; 42. a second rotating shaft; 43. a reaction card placing groove; 5. a reagent reaction card; 6. a transmission belt; 61. a card slot; 62. clamping the strip; 7. a pipe pressing device; 71. a pressure lever; 72. briquetting; 73. a limiting ring; 74. A return spring; 8. detecting a terminal; 81. a display screen; 82. a control button; 83. a power line; 9. a detection device; 91. and (4) optically detecting the sensing piece.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
The embodiment of the detection device for the abrus cantoniensis hance in the animal liver injury experiment refers to fig. 1-5, and comprises a shell 1, wherein the shell 1 is made of transparent material and is convenient for observing internal operation, a serum placing device 2 and a serum detection device 4 are rotationally connected in the shell 1, the serum placing device 2 comprises a serum placing disc 21, a first rotating shaft 22 which penetrates through the center of the serum placing disc 21 and is rotationally connected with the shell 1, six test tube placing holes 23 which are circumferentially distributed are formed in the serum placing disc 21, a test tube placing frame 24 is arranged at the lower end of each test tube placing hole 23, a hollow poking needle 25 is upwards arranged at the bottom of the frame which penetrates through each test tube 3, one end of each hollow poking needle 25, which is close to the serum placing disc 21, is in a needle point bevel opening shape, a test tube 3 with two hollow ends open is arranged in each test tube, the lower end of each test tube 3 is sealed by a saline bottle rubber plug 31, the hollow pricking needle 25 can prick the saline bottle rubber plug 31 seal, and the liquid in the test tube 3 is led out through the hollow pricking needle 25.
Serum detection device 4 places dish 41 including setting up in the reaction card of test tube placement frame 24 below, run through the reaction card and place dish 41 center and rotate the second pivot 42 of being connected with casing 1, reaction card places dish 41 and offers six reaction card standing grooves 43 that are the circumference and distribute and with test tube standing hole 23 one-to-one, two reagent reaction cards 5 have all been placed in each reaction card standing groove 43, these two reagent reaction cards 5 are glutamic-pyruvic transaminase (ALT) reagent reaction card 5 and glutamic-oxalacetic transaminase (AST) reagent reaction card 5.
A driving belt 6 is sleeved outside the first rotating shaft 22 and the second rotating shaft 42, a plurality of clamping grooves 61 which are distributed circumferentially are formed in the peripheries of the lower ends of the first rotating shaft 22 and the second rotating shaft 42, a plurality of clamping strips 62 matched with the clamping grooves 61 are arranged on the inner side of the driving belt 6, the serum placing disc 21 is rotated, the driving belt 6 drives the serum detecting disc to rotate, the serum placing disc 21 and the reaction card placing disc 41 rotate synchronously and do not deviate due to the clamping of the clamping grooves 61 and the clamping strips 62, one end of the shell 1 close to the serum placing device 2 and one end close to the serum detecting device 4 are provided with an opening, a through hole is formed in the upper end of the shell 1 relative to the upper ends of the serum placing disc 21 and the serum detecting disc, a pressure tube device 7 is inserted in the through hole and comprises a pressing rod 71 inserted in the through hole and a pressing block 72 connected with one end of the pressing rod, With depression bar 71 keep away from the spacing ring 73 that the one end of serum placing plate 21 is connected, install a cover between spacing ring 73 and the casing 1 and locate the outer reset spring 74 of depression bar 71, when test tube 3 rack rotated the briquetting 72 under, reaction card standing groove 43 rotated the test tube 3 rack under, pressed the test tube 3 of pipe device 7 below through pressing, can make cavity stab needle 25 stab brine bottle rubber buffer 31.
A detection terminal 8 is installed to the upper end of casing 1, be provided with a display screen 81 on detection terminal 8, install a controller (not shown in the figure) in the detection terminal 8, the inboard upper end of casing 1 is provided with a detection device 9 corresponding with reaction card standing groove 43 position, the lower extreme of detection device 9 is provided with a slice of optical detection response piece 91, display screen 81, optical detection response piece 91 all links with the controller electricity, one side of detection terminal 8 is provided with a power cord 83 that links with the controller electricity, make detection terminal 8 switch-on external power supply through power cord 83, be provided with a plurality of control button 82 that link with the controller electricity on the detection terminal 8.
The use method comprises the following steps: when serum is detected, firstly, the separated serum is respectively loaded into the test tubes 3, the serum placing tray 21 is manually rotated, each test tube 3 is placed in the corresponding test tube placing frame 24, and the alanine Aminotransferase (ALT) reagent reaction card 5 and the aspartate Aminotransferase (AST) reagent reaction card 5 are put into the reaction card placing groove 43 as a group;
then, rotating the serum placing disc 21 to enable one test tube 3 to be located below the tube pressing device 7, enabling the reaction card placing groove 43 to be located below the test tube 3, finely adjusting the serum placing disc 21 to enable the hollow puncturing needle 25 to be located above one reagent reaction card 5, pressing the tube pressing device 7, enabling the tube pressing device 7 to press down the test tube 3 to enable the hollow puncturing needle 25 to puncture the saline bottle rubber plug 31, enabling the serum in the test tube 3 to drip onto the reagent reaction card 5 through the hollow puncturing needle 25, enabling the reagent reaction card 5 to react with the serum to generate color change, and repeating the steps to enable the serum in each test tube 3 to react with the alanine Aminotransferase (ALT) reagent reaction card 5 and the aspartate Aminotransferase (AST) reagent reaction card 5;
finally, the serum placing disc 21 is rotated to enable the reagent reaction card 5 to be located under the optical detection sensing sheet 91, the optical detection sensing sheet 91 is enabled to detect the reagent reaction card 5 through the control button 82, detected information is transmitted to the controller, the controller processes the detected information and then displays the processed information through the display screen 81 to obtain a serum detection structure, the steps are repeated, each reagent reaction card 5 is detected, and serum detection is completed.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications to the present embodiment without inventive contribution as required after reading the present specification, but all of them are protected by patent laws within the scope of the claims of the present invention.
Claims (7)
1. The utility model provides a detection apparatus of abrus cantoniensis hance in animal liver injury experiment, includes casing (1), its characterized in that: the blood serum placing device is characterized in that a blood serum placing device (2) and a blood serum detecting device (4) are rotationally connected to the shell (1), the blood serum placing device (2) comprises a blood serum placing disc (21), a first rotating shaft (22) penetrates through the center of the blood serum placing disc (21) and is rotationally connected with the shell (1), a plurality of test tube placing holes (23) distributed circumferentially are formed in the blood serum placing disc (21), a test tube placing frame (24) is arranged at the lower end of each test tube placing hole (23), a hollow pricking needle (25) is upwards arranged at the bottom of each test tube (3) placing frame, test tubes (3) with openings at two ends are placed in each test tube placing frame (24), and the lower ends of the test tubes (3) are sealed by saline stoppers (31);
the serum detection device (4) comprises a reaction card placing disc (41) arranged below the test tube placing frame (24) and a second rotating shaft (42) penetrating through the center of the reaction card placing disc (41) and rotatably connected with the shell (1), a plurality of reaction card placing grooves (43) which are distributed in the circumferential direction and correspond to the test tube placing holes (23) one by one are formed in the reaction card placing disc (41), and reagent reaction cards (5) are placed in each reaction card placing groove (43);
a driving belt (6) is sleeved outside the first rotating shaft (22) and the second rotating shaft (42), openings are respectively arranged at one end of the shell (1) close to the serum placing device (2) and one end of the shell close to the serum detecting device (4), a through hole is formed in the upper end of the shell (1) opposite to the upper end of the serum placing disc (21) and the upper end of the serum detecting disc, a pressure tube device (7) is inserted into the through hole, the pressure tube device (7) comprises a pressure rod (71) inserted into the through hole, a pressing block (72) connected with one end of the pressure rod (71) close to the serum placing disc (21), and a limiting ring (73) connected with one end of the pressure rod (71) far away from the serum placing disc (21), and a reset spring (74) sleeved outside the pressure rod (71) is installed between the limiting ring (73) and the shell (1), when the test tube (3) placing frame rotates to the position under the pressing block (72), the reaction card placing groove (43) rotates to the position under the test tube (3) placing frame.
2. The detection device of the abrus herb in the animal liver injury experiment as claimed in claim 1, wherein: detection terminal (8) are installed to the upper end of casing (1), be provided with display screen (81) on detection terminal (8), install the controller in detection terminal (8), the upper end of casing (1) inboard be provided with reaction card standing groove (43) position corresponding detection device (9), the lower extreme of detection device (9) is provided with optical detection response piece (91), display screen (81), optical detection response piece (91) all with the controller electricity links.
3. The detection device of the abrus herb in the animal liver injury experiment as claimed in claim 1, wherein: a plurality of clamping grooves (61) which are distributed circumferentially are formed in the peripheries of the lower ends of the first rotating shaft (22) and the second rotating shaft (42), and a plurality of clamping strips (62) matched with the clamping grooves (61) are arranged on the inner side of the transmission belt (6).
4. The detection device of the abrus herb in the animal liver injury experiment as claimed in claim 1, wherein: one end of each hollow poking needle (25) close to the serum placing disc (21) is in a needle tip bevel opening shape.
5. The detection device of the abrus herb in the animal liver injury experiment as claimed in claim 2, wherein: and a power line (83) electrically connected with the controller is arranged on one side of the detection terminal (8).
6. The detection device of the abrus herb in the animal liver injury experiment as claimed in claim 2, wherein: and the detection terminal (8) is provided with a plurality of control buttons (82) electrically connected with the controller.
7. The detection device of the abrus herb in the animal liver injury experiment as claimed in claim 1, wherein: the reagent reaction card (5) is an alanine Aminotransferase (ALT) reagent reaction card (5) and an aspartate Aminotransferase (AST) reagent reaction card (5).
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CN201921373858.7U CN210690417U (en) | 2019-08-23 | 2019-08-23 | Detection apparatus for abrus cantoniensis hance in animal liver injury experiment |
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CN201921373858.7U CN210690417U (en) | 2019-08-23 | 2019-08-23 | Detection apparatus for abrus cantoniensis hance in animal liver injury experiment |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN112213301A (en) * | 2020-09-15 | 2021-01-12 | 四川行之智汇知识产权运营有限公司 | Collecting and detecting device for hemoglobin in blood |
CN114062263A (en) * | 2021-09-29 | 2022-02-18 | 新疆水清清环境监测技术服务有限公司 | Measuring instrument and measuring method for petroleum content in solid waste |
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2019
- 2019-08-23 CN CN201921373858.7U patent/CN210690417U/en active Active
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN112213301A (en) * | 2020-09-15 | 2021-01-12 | 四川行之智汇知识产权运营有限公司 | Collecting and detecting device for hemoglobin in blood |
CN114062263A (en) * | 2021-09-29 | 2022-02-18 | 新疆水清清环境监测技术服务有限公司 | Measuring instrument and measuring method for petroleum content in solid waste |
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