CN210683809U

CN210683809U - Dynamic cell culture device

Info

Publication number: CN210683809U
Application number: CN201921592000.XU
Authority: CN
Inventors: 郑盛; 杨涓
Original assignee: Third People's Hospital Of Yunnan Province
Current assignee: Third People's Hospital Of Yunnan Province
Priority date: 2019-09-24
Filing date: 2019-09-24
Publication date: 2020-06-05
Anticipated expiration: 2029-09-24

Abstract

The utility model relates to the technical field of experimental instruments, in particular to a dynamic cell culture device, which comprises a box body, wherein the left center of the box body is fixedly connected with a bearing plate, the center at the top of the bearing plate is fixedly connected with a vibrating motor, the output end of the vibrating motor runs through the left side of the inner wall of the box body and is fixedly connected with a storage plate, the top and the bottom at the left side of the inner wall of the box body, which are close to the center, are both fixedly connected with a limiting block, the center at the bottom of the inner wall of the box body is fixedly connected with a sleeve, the bottom of the inner wall of, the vibrating motor and the object placing plate are matched with the object placing groove, so that the object placing plate can vibrate up and down under the driving of the vibrating motor, a dynamic environment is provided for the incubator arranged in the object placing groove, the cells in the incubator are dynamically cultured, so that the accuracy and the scientificity of the experimental result of cell culture are improved.

Description

Dynamic cell culture device

Technical Field

The utility model relates to an experimental apparatus technical field specifically is a developments cell culture device.

Background

Cell culture refers to a method of simulating in vivo environment in vitro to enable the cell to survive, grow, reproduce and maintain the main structure and function, and is also called cell cloning technology, the formal term in biology is cell culture technology, which is an important and common technology in research methods of cell biology, and a large number of cells can be obtained through cell culture, and signal transduction, cell anabolism, cell growth and reproduction and the like of the cells can be researched.

In animal body, tissue fluid can flow, so that the cell is living in dynamic environment, the state and function of the cell cultured on static cell culture device are different from those cultured under dynamic condition, the experimental results may be different from the results of cell detection in animal bodies, so it is necessary to use a dynamic cell culture apparatus to culture cells in a dynamic environment, maintain normal functions, differentiation, secretion, etc. of the cells, and improve the accuracy and scientificity of the experimental results, and the existing dynamic cell culture apparatus generally places a cell incubator on a platform, then the cell culture device vibrates up and down or simulates animal movement, which can improve the accuracy of cell culture, however, the incubator of cells is easily dropped from the platform during shaking, causing necrosis or damage to the cells and causing contamination to the surrounding environment.

SUMMERY OF THE UTILITY MODEL

The utility model provides a not enough to prior art, the utility model provides a developments cell culture device possesses can the dynamic culture cell, fixes advantages such as firm between incubator and the platform, has solved traditional static culture device and has cultivateed unable dynamic culture cell, fixed insecure problem between incubator and the platform.

The utility model discloses a dynamic cell culture device, which comprises a box body, the left central fixedly connected with bearing plate of box body, the central fixedly connected with vibrating motor at the top of bearing plate, the output end of vibrating motor runs through the left side of the inner wall of box body and is fixedly connected with a placing plate, the structure is arranged, so that the placing plate can vibrate up and down under the driving of vibrating motor, thereby providing a dynamic environment for the incubator arranged in the placing groove, dynamically culturing the cells in the incubator, improving the accuracy and scientificity of the experimental result of cell culture, the top and the bottom of the left side of the inner wall of box body near the central are fixedly connected with a limiting block, the central fixedly connected with a sleeve at the bottom of the inner wall of box body, the bottom of the inner wall of sleeve is movably connected with a slide bar, the bottoms of the left and the right sides of slide bar are fixedly connected with a second, second slider sliding connection is in the second spout, the top of slide bar and the top of putting the thing board fixed connection mutually, and this structure setting for when putting the thing board shake from top to bottom, also can stimulate the slide bar and vibrate from top to bottom, prevent to put the thing board because vibration amplitude is too big, drop from vibrating motor's output, improved the security of device.

The utility model discloses a developments cell culture device, wherein put the top of thing board and seted up and put the thing groove, put the thing groove along the horizontal direction equidistance of putting the thing board and set up, it has the push rod to peg graft in the middle part activity of thing tank bottom, the bottom of push rod runs through the bottom of putting the thing board, the bottom of push rod and with the position fixedly connected with fixed block of putting the mutual laminating in bottom of thing board, the equal threaded connection in left and right sides of fixed block bottom has fixing bolt, fixing bolt's top run through the top of fixed block and with put the bottom of thing board and laminate each other, this structure sets up, when the push rod is putting the thing board and trembles from top to bottom, can not shake from top to bottom along with the push rod, after cell culture accomplishes, can pass through the dismantlement bolt.

The utility model discloses a developments cell culture device, wherein the first slider of the equal fixedly connected with in central authorities of the push rod left and right sides, put the left and right sides of thing tank bottom portion and all seted up first spout, first slider sliding connection is in first spout, and this structure sets up for the staff can release the incubator from putting the thing inslot through promoting the push rod, so that the state of cell after the observation has been cultivateed has improved staff's work efficiency.

The utility model discloses a developments cell culture device, wherein put the equal fixedly connected with spring of thing groove inner wall left and right sides and front and hou mian central position department, the spring is close to the central fixedly connected with fixture block of putting thing groove center one side, and the fixture block is close to one side of putting thing groove center and runs through the outer wall of putting the thing groove, and this structure sets up for the incubator is put into put thing inslot back into, can be fixed more stable, has improved the stability of incubator after being fixed.

The utility model discloses a developments cell culture device, wherein the bottom activity on box inner wall right side is pegged graft and is had the glass door, the left top fixedly connected with handle of glass door, and this structure sets up for the state when cell is cultivateed can be observed to the staff, thereby makes staff's work convenience more.

Compared with the prior art, the beneficial effects of the utility model are as follows:

1. the utility model discloses a vibrating motor who sets up with put the thing board, the thing groove is put in the cooperation, make to put the thing board can vibrate from top to bottom under vibrating motor's drive, thereby the incubator to putting the thing inslot and placing provides a dynamic environment, carry out the dynamic culture to the cell in the incubator, the accuracy and the scientificity of the experimental result of cell culture have been improved, second slider and slide bar through setting up, the cooperation sleeve, when making to put the thing board shake from top to bottom, also can stimulate the slide bar and vibrate from top to bottom, prevent to put the thing board because vibration range is too big, the output from vibrating motor drops, the security of device has been improved, spring and fixture block through setting, make the incubator after being put into the thing inslot, can be fixed more stable, the stability of incubator after being fixed has been improved.

2. The utility model discloses a push rod and the first slider that set up, make the staff can push the incubator from putting the thing inslot through promoting the push rod and release, so as to observe the state of cultivateing the back cell, improve staff's work efficiency, through the stopper that sets up, make vibrating motor's vibration amplitude can receive the restriction, prevent vibrating motor amplitude too big, lead to putting the thing board and break away from vibrating motor's output, the security of device has further been improved, through the fixed block and the fixing bolt that set up, when making the push rod shake from top to bottom putting the thing board, can not shake from top to bottom along with the push rod, after the cell culture is accomplished, can push the push rod through dismantling the bolt, then promote the incubator of putting the thing inslot to push out, staff's work efficiency has been improved, through glass door and the handle that sets up, make the staff can be more convenient observe the state when cell culture, thereby the work of the staff is more convenient.

Drawings

The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the application and together with the description serve to explain the application and not to limit the application. In the drawings:

FIG. 1 is a schematic view of the overall front view cross-section structure of the present invention;

FIG. 2 is a schematic view of the top cross-sectional structure of the storage tank of the present invention;

fig. 3 is an enlarged schematic view of a portion a in fig. 1.

In the figure: 1. a box body; 2. a bearing plate; 3. a vibration motor; 4. a limiting block; 5. a storage plate; 6. a storage groove; 7. a push rod; 8. a fixed block; 9. fixing the bolt; 10. a first slider; 11. a first chute; 12. a sleeve; 13. a slide bar; 14. a second slider; 15. a second chute; 16. a spring; 17. a clamping block; 18. a glass door; 19. a handle.

Detailed Description

In the following description, numerous implementation details are set forth in order to provide a more thorough understanding of the present invention. It should be understood, however, that these implementation details should not be used to limit the invention. That is, in some embodiments of the invention, details of these implementations are not necessary. In addition, some conventional structures and components are shown in simplified schematic form in the drawings.

In addition, the descriptions related to "first", "second", etc. in the present invention are only for description purposes, not specifically referring to the order or sequence, and are not intended to limit the present invention, but only to distinguish the components or operations described in the same technical terms, and are not to be construed as indicating or implying any relative importance or implicit indication of the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, the technical solutions in the embodiments may be combined with each other, but it must be based on the realization of those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should not be considered to exist, and is not within the protection scope of the present invention.

Referring to fig. 1-3, the dynamic cell culture apparatus of the present invention comprises a box body 1, a bearing plate 2 fixedly connected to the left center of the box body 1, a vibrating motor 3 fixedly connected to the center of the top of the bearing plate 2, the vibrating motor 3 being JZO-0.7-2 type vibrating motor, an output end of the vibrating motor 3 penetrating the left side of the inner wall of the box body 1 and fixedly connected to a storage plate 5, the structure being such that the storage plate 5 can vibrate up and down under the driving of the vibrating motor 3, thereby providing a dynamic environment for the incubator placed in the storage tank 6, dynamically culturing cells in the incubator, improving the accuracy and scientificity of the experimental results of cell culture, a stopper 4 fixedly connected to the top and bottom of the left side of the inner wall of the box body 1 and close to the center, a sleeve 12 fixedly connected to the center of the bottom of the inner wall of the box body 1, the bottom swing joint of sleeve 12 inner wall has slide bar 13, the equal fixedly connected with second slider 14 in bottom of the slide bar 13 left and right sides, second spout 15 has all been seted up to the left and right sides of sleeve 12 inner wall, 14 sliding connection of second slider are in second spout 15, slide bar 13's top and the top looks fixed connection who puts thing board 5, the structure sets up, make when putting thing board 5 from top to bottom the shake, also can stimulate slide bar 13 vibration from top to bottom, it is too big to prevent to put thing board 5 because the vibration amplitude, the output from vibrating motor 3 drops, the security of device has been improved.

Put the top of thing board 5 and seted up and put thing groove 6, put thing groove 6 and set up along the horizontal direction equidistance who puts thing board 5, the central authorities activity of putting thing groove 6 bottom is pegged graft and is had push rod 7, the bottom of push rod 7 runs through the bottom of putting thing board 5, the bottom of push rod 7 and with put the position department fixedly connected with fixed block 8 of thing board 5's bottom laminating each other, the equal threaded connection in left and right sides of fixed block 8 bottom has fixing bolt 9, fixing bolt 9's top runs through the top of fixed block 8 and laminates each other with the bottom of putting thing board 5, the structure sets up, when making push rod 7 shake about putting thing board 5, can not shake from top to bottom along with push rod 7, after the cell culture is accomplished, can pass through the detaching bolt, then promote push rod 7, it is ejecting to put the incubator in the.

The first slider 10 of the equal fixedly connected with in central authorities of the 7 left and right sides of push rod, put the left and right sides of thing groove 6 bottom and all seted up first spout 11, and first slider 10 sliding connection is in first spout 11, and this structure sets up for the staff can release the incubator from putting thing groove 6 in through promoting push rod 7, so that observe the state of cultivateing the back cell, improved staff's work efficiency.

Put the equal fixedly connected with spring 16 of thing groove 6 inner wall left and right sides and front and hou mian central point department of putting, spring 16 is close to the central fixedly connected with fixture block 17 of putting thing groove 6 center one side, and one side that fixture block 17 is close to putting thing groove 6 center runs through the outer wall of putting thing groove 6, and this structure sets up for the incubator is put into put thing groove 6 back in, can be fixed more stable, has improved the stability of incubator after being fixed.

The bottom activity on box 1 inner wall right side is pegged graft and is had glass door 18, and the left top fixedly connected with handle 19 of glass door 18, this structure setting for the state when the staff can observe the cell and is cultivateed, thereby makes staff's work more convenient.

When using the utility model discloses the time: the worker firstly pulls the handle 19 upwards to drive the glass door 18 to move upwards, and places the incubator of cells in each storage slot 6 of the storage plate 5, at the moment, the bottom of the incubator extrudes the fixture block 17 at the bottom of the storage slot 6, then the fixture block 17 extrudes the spring 16 to compress the spring 16, the spring 16 gives an opposite acting force to the incubator to firmly fix the incubator in the storage slot 6, then the fixing bolt 9 is screwed down to prevent the push rod 7 from sliding, then the glass door 18 is mutually attached to the bottom of the box body 1, and the vibration motor 3 starts to work, the output end of the vibration motor 3 vibrates up and down to drive the storage plate 5 to vibrate up and down, the slide rod 13 at the bottom is pulled to slide up and down while the storage plate 5 vibrates up and down, the second slide block 14 at the bottom of the slide rod 13 slides in the second slide slot 15 to reduce the gravity on the storage plate 5 during vibration, and prevent the storage plate 5 from, the incubator vibrates about putting thing groove 6 is same, provide a dynamic cultivation environment for the incubator, guarantee the accuracy after the cell culture, after the cultivation is accomplished, the staff upwards stimulates handle 19, reopen glass door 18, and screw off fixing bolt 9, then upwards promote push rod 7, drive first slider 10 and slide in first spout 11, release the incubator from putting thing groove 6 is interior, then carry out analysis and data collection to the cell in the incubator, the completion is to the dynamic cultivation of cell.

The above description is only an embodiment of the present invention, and is not intended to limit the present invention. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.

Claims

1. A dynamic cell culture device comprises a box body (1), and is characterized in that: the center of the left side of the box body (1) is fixedly connected with a bearing plate (2), the center of the top of the bearing plate (2) is fixedly connected with a vibration motor (3), the output end of the vibrating motor (3) penetrates through the left side of the inner wall of the box body (1) and is fixedly connected with a storage plate (5), the top and the bottom of the left side of the inner wall of the box body (1) and near the center are both fixedly connected with a limiting block (4), the center of the bottom of the inner wall of the box body (1) is fixedly connected with a sleeve (12), the bottom of the inner wall of the sleeve (12) is movably connected with a slide bar (13), the bottoms of the left side and the right side of the slide bar (13) are fixedly connected with second slide blocks (14), the left side and the right side of the inner wall of the sleeve (12) are both provided with a second sliding chute (15), the second sliding block (14) is connected in the second sliding groove (15) in a sliding mode, and the top of the sliding rod (13) is fixedly connected with the top of the storage plate (5).

2. The dynamic cell culture device according to claim 1, wherein: the top of the object placing plate (5) is provided with object placing grooves (6), and the object placing grooves (6) are arranged at equal intervals along the horizontal direction of the object placing plate (5).

3. A dynamic cell culture device according to claim 2, wherein: the movable central inserting type storage box is characterized in that a push rod (7) is movably inserted into the center of the bottom of the storage groove (6), the bottom of the push rod (7) penetrates through the bottom of the storage plate (5), a fixed block (8) is fixedly connected with the bottom of the push rod (7) and the position, which is mutually attached to the bottom of the storage plate (5), of the bottom of the fixed block (8), fixing bolts (9) are connected to the left side and the right side of the bottom of the fixed block (8) in a threaded mode, and the top of each fixing bolt (9) penetrates through the top of the fixed block (8) and is mutually.

4. A dynamic cell culture device according to claim 3, wherein: the middle of the left side and the right side of the push rod (7) are fixedly connected with first sliding blocks (10), the left side and the right side of the bottom of the storage groove (6) are provided with first sliding grooves (11), and the first sliding blocks (10) are connected in the first sliding grooves (11) in a sliding mode.

5. The dynamic cell culture device according to claim 4, wherein: put the equal fixedly connected with spring (16) of thing groove (6) inner wall left and right sides and front and the rear central point department of putting, spring (16) are close to central fixedly connected with fixture block (17) of putting thing groove (6) center one side, one side that fixture block (17) are close to putting thing groove (6) center runs through the outer wall of putting thing groove (6).

6. The dynamic cell culture device according to claim 1, wherein: the bottom on the right side of the inner wall of the box body (1) is movably inserted with a glass door (18), and the top on the left side of the glass door (18) is fixedly connected with a handle (19).