WO2006048783A3 - Autogenic living scaffolds and living tissue matrices: methods and uses thereof - Google Patents

Autogenic living scaffolds and living tissue matrices: methods and uses thereof Download PDF

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Publication number
WO2006048783A3
WO2006048783A3 PCT/IB2005/004091 IB2005004091W WO2006048783A3 WO 2006048783 A3 WO2006048783 A3 WO 2006048783A3 IB 2005004091 W IB2005004091 W IB 2005004091W WO 2006048783 A3 WO2006048783 A3 WO 2006048783A3
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Prior art keywords
cells
tissue
living
als
matrix
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PCT/IB2005/004091
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French (fr)
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WO2006048783A2 (en
WO2006048783A8 (en
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Jan-Eric W Ahlfors
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Jan-Eric W Ahlfors
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Priority to CA2559275A priority Critical patent/CA2559275C/en
Priority to EP05849047A priority patent/EP1727893B1/en
Priority to AU2005300263A priority patent/AU2005300263A1/en
Priority to JP2007502444A priority patent/JP2007528252A/en
Publication of WO2006048783A2 publication Critical patent/WO2006048783A2/en
Publication of WO2006048783A8 publication Critical patent/WO2006048783A8/en
Publication of WO2006048783A3 publication Critical patent/WO2006048783A3/en

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656—Adult fibroblasts
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Abstract

A 3-dimensional structure comprising suitable cells (or entities) and the ECM (or matrix) that has been completely produced and arranged by these cells (or entities) that promotes the differentiation, dedifferentiation and/or transdifferentiation of cells and/or formation of tissue in vitro and in vivo, while at the same time promoting cell growth, proliferation, migration, acquisition of in vivo-like morphology, or combinations thereof, and that 1. provides structural and/or nutritional support to cells, tissue, organs, or combinations thereof, termed an 'Autogenic Living Scaffold' (ALS); or 2. is capable of being transformed into a more complex tissue (or matrix) or a completely different type of tissue (or matrix), termed a 'Living Tissue Matrix' (LTM). Autogenic means it is self-produced. The living cells that produce the LTM or ALS, or are added to Autogenic Living Scaffolds, may be genetically engineered or otherwise modified. The matrix component of the ALS or LTM provides a structural framework for cells that guide their direction of growth, enables them to be correctly spaced, prevents overcrowding, enables cells to communicate between each other, transmit subtle biological signals, receive signals from their environment, form bonds and contacts that are required for proper functioning of all cells within a unit such as a tissue, or combinations thereof. The ALS or LTM may thus provide proper or supporting mechanical and chemical environments, signals, or stimuli to other cells, to the cells that produce the ALS, to surrounding tissue at an implantation site, to a wound, for in vitro and ex vivo generation and regeneration of cells, tissue and organs, or combinations thereof. They may also provide other cells with nutrients, growth factors, and/or other necessary or useful components. They may also take in or serve as buffers for certain substances in the environment, and have also some potential at adapting to new environments.
PCT/IB2005/004091 2004-03-09 2005-03-09 Autogenic living scaffolds and living tissue matrices: methods and uses thereof WO2006048783A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA2559275A CA2559275C (en) 2004-03-09 2005-03-09 Autogenic living scaffolds and living tissue matrices: methods and uses thereof
EP05849047A EP1727893B1 (en) 2004-03-09 2005-03-09 Autogenic living scaffolds and living tissue matrices: methods and uses thereof
AU2005300263A AU2005300263A1 (en) 2004-03-09 2005-03-09 Autogenic living scaffolds and living tissue matrices: methods and uses thereof
JP2007502444A JP2007528252A (en) 2004-03-09 2005-03-09 Autogenesis bioscaffolding and tissue matrix; methods and uses thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US55143104P 2004-03-09 2004-03-09
US60/551,431 2004-03-09
US93067304A 2004-08-30 2004-08-30
US10/930,673 2004-08-30

Publications (3)

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WO2006048783A2 WO2006048783A2 (en) 2006-05-11
WO2006048783A8 WO2006048783A8 (en) 2006-08-24
WO2006048783A3 true WO2006048783A3 (en) 2006-11-09

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Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101332315A (en) * 2007-06-29 2008-12-31 上海国睿生命科技有限公司 Tissue engineering tendon and vitro construction method thereof
US8926552B2 (en) 2009-08-12 2015-01-06 Medtronic, Inc. Particle delivery
US20190100725A1 (en) * 2016-03-18 2019-04-04 Institute Of Biophysics, Chinese Academy Of Sciences Cell culture medium and culture medium supplement

Citations (4)

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EP0282746A1 (en) * 1987-02-19 1988-09-21 Takeda Chemical Industries, Ltd. Method for producing artificial cultured tissue
EP1131410A1 (en) * 1998-11-19 2001-09-12 Organogenesis Inc. Bioengineered tissue constructs and methods for producing and using them
WO2002020729A2 (en) * 2000-09-06 2002-03-14 UNIVERSITé LAVAL In vitro human angiogenesis model
WO2003018767A2 (en) * 2001-08-27 2003-03-06 Advanced Cell Technology, Inc. Trans-differentiation and re-differentiation of somatic cells and production of cells for cell therapies

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US6995013B2 (en) 2002-07-08 2006-02-07 Biomed Solutions, Llc Cell-scaffold composition containing five layers

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EP0282746A1 (en) * 1987-02-19 1988-09-21 Takeda Chemical Industries, Ltd. Method for producing artificial cultured tissue
EP1131410A1 (en) * 1998-11-19 2001-09-12 Organogenesis Inc. Bioengineered tissue constructs and methods for producing and using them
WO2002020729A2 (en) * 2000-09-06 2002-03-14 UNIVERSITé LAVAL In vitro human angiogenesis model
WO2003018767A2 (en) * 2001-08-27 2003-03-06 Advanced Cell Technology, Inc. Trans-differentiation and re-differentiation of somatic cells and production of cells for cell therapies

Non-Patent Citations (5)

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Title
AHLFORS, JAN-ERIC: "A Comparative Analysis of the Biomechanics and Biochemistry of Cell-Derived and Cell-Remodeled Matrices: Implications for Wound Healing and Regenerative Medicine", 24 April 2004 (2004-04-24), thesis presentation, XP002391632, Retrieved from the Internet <URL:HTTP://WWW.WPI.EDU/PUBS/ETD/AVAILABLE/ETD-0503104-172949/> *
GOSPODAROWICZ D ET AL: "STRUCTURAL CHARACTERIZATION AND BIOLOGICAL FUNCTIONS OF FIBROBLAST GROWTH FACTOR", ENDOCRINE REVIEWS, BALTIMORE, MD, US, vol. 8, no. 2, 1987, pages 95 - 114, XP009003095 *
MICHEL M ET AL: "Characterization of a new tissue-engineered human skin equivalent with hair.", IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. ANIMAL. JUN 1999, vol. 35, no. 6, June 1999 (1999-06-01), pages 318 - 326, XP008067234, ISSN: 1071-2690 *
MURAKAMI TAKESHI ET AL: "Transplanted neuronal progenitor cells in a peripheral nerve gap promote nerve repair.", BRAIN RESEARCH. 6 JUN 2003, vol. 974, no. 1-2, 6 June 2003 (2003-06-06), pages 17 - 24, XP002392826, ISSN: 0006-8993 *
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CA2559275C (en) 2020-02-18
CA2559275A1 (en) 2006-05-11
WO2006048783A2 (en) 2006-05-11
WO2006048783A8 (en) 2006-08-24

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