CN210665463U - Saliva urease activity detects box - Google Patents

Abstract

The utility model relates to a saliva urease activity detection box, which is characterized in that the box comprises a plunger rod, a hollow cylinder and a narrow-mouth bottle, and the plunger rod, the hollow cylinder and the narrow-mouth bottle can be screwed, screwed and fixedly connected from top to bottom; the plunger rod comprises five parts, namely a solid plunger rod, a hollow plunger rod, a solid cone, a cover and a plastic shell; the solid plunger rod, the hollow plunger rod and the solid cone are connected from top to bottom and are integrated and can not be detached, and the hollow plunger rod and the solid cone are wrapped by the plastic shell; wherein the solid plunger rod is provided with a red shift line; wherein the top end of the cover is hermetically connected with the lower edge of the plastic shell; the bottle body of the narrow-mouth bottle is transparent, and the bottle mouth is plastically packaged by thin aluminum foil, thin plastic or materials mixed according to different proportions. The utility model discloses simple structure, convenient to use and with low costs can detect urease's activity in the saliva fast directly perceivedly, make things convenient for the customer to use.

Description

Saliva urease activity detects box

Technical Field

The utility model belongs to the short-term test field relates to an utilize the reaction product of urease and urea in the saliva to cause the short-term test box of urease activity in the test solution colour change coming semi-quantitative saliva.

Background

Urease is a nickel-containing oligomeric enzyme, and can be produced by bacteria such as streptococcus salivarius, actinomyces naeslundii, staphylococcus wowensis, helicobacter pylori and the like which may be contained in oral cavity. Urease can cause various human diseases, including dental caries, periodontitis and the like related to the oral cavity, and other common symptoms are gastritis, pyelonephritis, lithangiuria and the like. In general, urease in the oral cavity is considered a diagnostic biomarker that can be used to assess early oral and systemic disease. Urease can specifically catalyze urea hydrolysis and cause solution pH changes, based on which the activity of urease in saliva can be semi-quantified using phenolphthalein indicators. However, no suitable cartridge is currently used for this method. Therefore, the invention provides a detection kit for urease activity in saliva, which has important significance.

Disclosure of Invention

The invention aims to provide a saliva urease activity detection kit, which can realize the aim of measuring the activity of urease in saliva by a visual method by using the principle that the color change of a test solution is triggered by a reaction product of urease in the saliva and urea and only needing to quantitatively add the collected saliva into the test solution, uniformly mix and then stand for a proper time.

In order to realize the purpose of the invention, the technical scheme of the invention is as follows:

1. a saliva urease activity detection box is characterized by comprising three parts, namely a plunger rod, a hollow cylinder and a narrow-mouth bottle; the plunger rod, the hollow cylinder and the narrow-mouth bottle can be screwed and fixedly connected from top to bottom.

2. The plunger rod comprises five parts, namely a solid plunger rod, a hollow plunger rod, a solid cone, a cover and a plastic shell; the solid plunger rod, the hollow plunger rod and the solid cone are connected into a whole from top to bottom and cannot be detached, and the hollow plunger rod and the solid cone are wrapped by a plastic shell; the solid plunger rod is provided with a red shift line, the distance between the solid plunger rod and the top end of the plastic shell is the same as the height of the plastic shell, and the length of the solid plunger rod is not less than 2.6 times of the height of the plastic shell; small holes are uniformly distributed on the outer wall of the hollow plunger rod, and the inner volume of the hollow plunger rod is fixed; the top end of the cover is connected with the lower edge of the plastic shell in a sealing way, the center of the cover is provided with a hollow part with the shape identical to the shape of the plunger rod, and the inner wall of the cover is provided with threads.

3. The outer wall of the top end of the hollow cylinder is provided with threads for connecting the plunger rod; the inner wall of the bottom end of the hollow partition board is provided with a thread for connecting the narrow-mouth bottle, and the top end of the thread is provided with a partition board with a hollow center shape which is the same as the shape of the plunger rod; the height of the hollow cylinder except the bottom end thread is equivalent to the sum of the heights of the hollow plunger rod and the solid cone.

4. The outer wall of the bottle mouth of the narrow-mouth bottle is provided with threads, the volume of the narrow-mouth bottle is not less than 10mL, the bottle mouth is sealed by thin aluminum foil and thin plastic or materials mixed according to different proportions in a plastic package mode, and the bottle body is transparent.

Has the advantages that: according to the invention, the collected saliva is quantitatively added into the narrow-mouth bottle containing the test solution, so that the visual determination of the activity of the urease in the saliva can be realized. The invention does not need pretreatment process, has simple and convenient operation, convenient carrying and transportation, lower cost and higher convenient application performance, and can meet the requirements of customers.

Drawings

FIG. 1 is a diagram showing a structure of a box for detecting the activity of a sialidase

Wherein: 1, a plunger rod; 8, a hollow cylinder; 9, narrow-mouth bottle.

FIG. 2 is an exploded view of the respective parts of a urease activity detecting cassette

FIG. 3, the plunger rod structure

Wherein: 2, a solid plunger rod; 3, red shift line; 4, a plastic shell; 5, a hollow plunger rod; 6, a solid cone; 7, and a cover.

FIG. 4 is a schematic view of the present invention

Detailed Description

The present invention is further illustrated by the following examples, but is not limited thereto in any way, and any variations or modifications based on the teachings of the present invention are within the scope of the present invention.

Example one

Before use, the hollow cylinder (8) is screwed onto the narrow-mouth bottle (9), the collected saliva is added to the hollow cylinder (8) to full, and then the plunger rod (1) is screwed onto the hollow cylinder (8). And (3) pressing the plunger rod (1) downwards to a gear line (3), so that the hollow plunger rod (5) penetrates through the hollow part in the middle of the cover (7) and completely enters the hollow cylinder (8), and when saliva completely enters the hollow plunger rod (5), quickly pressing the plunger rod (1) downwards, so that the hollow plunger rod (5) penetrates through the partition plate at the lower layer of the hollow cylinder (8), penetrates through the thermoplastic seal of the thin-mouth bottle (9), is completely immersed into the test solution, and is shaken and uniformly mixed. After standing for a suitable time, the test solution in the narrow-mouth bottle was observed for color change.

Claims (4)

1. A saliva urease activity detection box is characterized by comprising three parts, namely a plunger rod (1), a hollow cylinder (8) and a narrow-mouth bottle (9); the plunger rod (1), the hollow cylinder (8) and the narrow-mouth bottle (9) can be screwed and fixedly connected from top to bottom.

2. The saliva urease activity test box according to claim 1, wherein the plunger rod (1) comprises five parts, namely a solid plunger rod (2), a hollow plunger rod (5), a solid cone (6), a cover (7) and a plastic shell (4); the solid plunger rod (2), the hollow plunger rod (5) and the solid cone (6) are connected into a whole from top to bottom and cannot be disassembled, and the hollow plunger rod (5) and the solid cone (6) are wrapped by the plastic shell (4); a red shift line (3) is arranged on the solid plunger rod (2), the distance between the solid plunger rod and the top end of the plastic shell (4) is the same as the height of the plastic shell (4), and the length of the solid plunger rod (2) is not less than 2.6 times of the height of the plastic shell (4); small holes are uniformly distributed on the outer wall of the hollow plunger rod (5), and the inner volume of the hollow plunger rod is fixed; the top end of the cover (7) is connected with the lower edge of the plastic shell (4) in a sealing way, the center of the cover is provided with a hollow part with the shape identical to that of the plunger rod (1), and the inner wall of the cover (7) is provided with threads.

3. A urease activity test cartridge according to claim 1, wherein the hollow cylinder (8) has a thread on the top outer wall for connecting the plunger rod (1); the inner wall of the bottom end of the plunger rod is provided with a thread for connecting a narrow-mouth bottle (9), and the top end of the thread is provided with a clapboard with the hollow center shape which is the same as the shape of the plunger rod (1); the height of the hollow cylinder (8) except the bottom end thread is equivalent to the sum of the heights of the hollow plunger rod (5) and the solid cone (6).

4. The activity detection kit of a sialyase according to claim 1, characterized in that the outer wall of the mouth of the narrow-mouth bottle (9) is threaded, the volume is not less than 10mL, the mouth is plastic-sealed by thin aluminum foil, thin plastic or materials mixed in different proportions, and the bottle body is transparent.

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