CN210620842U - Multi-functional cell culture liquid mixing arrangement for cell engineering - Google Patents

Multi-functional cell culture liquid mixing arrangement for cell engineering Download PDF

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Publication number
CN210620842U
CN210620842U CN201921548241.4U CN201921548241U CN210620842U CN 210620842 U CN210620842 U CN 210620842U CN 201921548241 U CN201921548241 U CN 201921548241U CN 210620842 U CN210620842 U CN 210620842U
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China
Prior art keywords
mixing
middle position
culture solution
detachable
pipe
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Expired - Fee Related
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CN201921548241.4U
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Chinese (zh)
Inventor
浠诲嘲
任峰
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Tianjin Stm Biotechnology Co ltd
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Tianjin Stm Biotechnology Co ltd
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Abstract

The utility model provides a multi-functional cell culture liquid mixing arrangement for cell engineering, including culture solution advance fill, the honeycomb duct, the blending tank moves the frame, the calandria, mechanical valve, the supporting leg removes the wheel, adsorbs the piece, collects the box, the handle, control switch, detachable regulation mixing leaf structure and detachable filtration derivation core structure. The utility model discloses the setting of hybrid motor, regulation seat, mixing shaft and removable mixing leaf is favorable to improving mixing efficiency, guarantees the mixed effect; the arrangement of the adjusting pipe, the supporting rod, the square-head bolt and the movable handle is beneficial to adjusting the position of the adjusting seat according to the mixing requirement so as to ensure the mixing effect; the setting of delivery pipe, clearance bottom, a back cover section of thick bamboo and straining miscellaneous core is favorable to straining miscellaneous to the culture solution of taking out, can guarantee to use the convenience, can guarantee to detect the accuracy simultaneously.

Description

Multi-functional cell culture liquid mixing arrangement for cell engineering
Technical Field
The utility model belongs to the technical field of cell engineering, especially, relate to a multi-functional cell culture liquid mixing arrangement for cell engineering.
Background
Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technology can be used for culturing a single cell into simple single cells or rarely differentiated multiple cells in a large scale, which is an indispensable link of the cloning technology. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched.
At present, a culture solution suitable for cells to live outside is required to be used for cell culture, various substances required by the growth of various cells are mixed in the culture solution, and scientific researchers need to add nutrient substances into tissue fluid when preparing the cell culture solution and then shake and mix the tissue fluid uniformly to mix the tissue fluid uniformly.
However, the existing mixing device has the problems of low mixing efficiency, no impurity guiding and filtering function and inconvenience in movement.
Therefore, it is necessary to invent a multifunctional cell culture solution mixing device for cell engineering.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model provides a multi-functional cell culture liquid mixing arrangement for cell engineering to there is the mixing efficiency low in solving current mixing arrangement, does not possess the problem of deriving and straining miscellaneous function and be not convenient for remove. A multifunctional cell culture solution mixing device for cell engineering comprises a culture solution inlet hopper, a flow guide pipe, a mixing barrel, a moving base, a discharge pipe, a mechanical valve, a supporting leg, a moving wheel, an adsorption sheet, a collection box, a lifting handle, a control switch, a detachable adjusting mixing blade structure and a detachable filtering guide core structure, wherein the middle position of the lower part of the culture solution inlet hopper is in threaded connection with the upper right end of the flow guide pipe; the left lower end of the flow guide pipe is in threaded connection with an inlet at the upper right side of the mixing barrel; the longitudinal bottom of the mixing barrel is connected with the middle position of the upper part of the movable base through a bolt; the upper part of the calandria in the longitudinal direction penetrates through the middle position in the moving machine base and is in threaded connection with an outlet at the middle position at the bottom of the mixing barrel; the mechanical valve is in threaded connection with the middle position of the front part of the discharge pipe; the supporting legs are respectively connected to the four corners of the bottom of the movable base through bolts at one longitudinal end, and are connected to the middle position of the upper part of the movable wheel through bolts at the other end; the adsorption pieces are respectively and longitudinally adsorbed at the right lower side of the supporting leg arranged at the right side; the adsorption pieces are respectively connected to the front part and the rear part of the left side of the collection box by longitudinal bolts; a handle is connected with the middle position of the right part of the collecting box through a screw; the control switch is connected to the middle position of the left side of the front part of the movable base through a transverse bolt; the detachable adjusting mixing blade structure and the detachable filtering leading-out core structure are respectively connected with the mixing barrel; the detachable adjusting mixing blade structure comprises a mixing motor, an adjusting seat, a mixing shaft, a detachable mixing blade, an adjusting pipe, a supporting rod, a square-head bolt and a moving handle, wherein the longitudinal lower end of the mixing motor is connected to the middle position of the upper part of the adjusting seat through the bolt; the longitudinal upper end of the mixing shaft penetrates through the middle position inside the adjusting seat; the coupling at the longitudinal upper end of the mixing shaft is connected to the lower end of the output shaft of the mixing motor; the detachable mixing blade is sleeved on the lower part of the outer wall of the mixing shaft from top to bottom in sequence; the adjusting seat is connected with the middle position of the right end of the adjusting pipe by a bolt at the left end; the adjusting pipe is sleeved on the outer wall of the supporting rod and is fixedly connected with the supporting rod through a square-head bolt; the right end of the movable handle is connected to the middle position of the left side of the adjusting pipe through a bolt.
Preferably, the detachable filtering and guiding core structure comprises a discharge pipe, a cleaning bottom cover, a bottom sealing barrel, a impurity filtering core, a control pipe, a connecting sleeve and a control valve, wherein the longitudinal upper end of the discharge pipe is in threaded connection with an outlet at the middle position of the lower end of the cleaning bottom cover; the cleaning bottom cover is in threaded connection with an outlet at the lower end of the bottom sealing barrel; the impurity filtering core is longitudinally arranged inside the bottom sealing barrel, and the lower part of the impurity filtering core is connected with the cleaning bottom cover through a screw; one end of the control pipe is in threaded connection with an inlet at the middle position of the top of the bottom sealing barrel, and the other end of the control pipe is in threaded connection with the right end of the discharge pipe; the control valve is in threaded connection with the middle position of the left lower side of the control pipe.
Preferably, the adsorption piece adopts two permanent magnet pieces.
Preferably, the mixing shaft and the detachable mixing blade are arranged in a bolt connection mode.
Preferably, the detachable mixing blade adopts a plurality of stainless steel spiral blades.
Preferably, the lower end of the support rod in the longitudinal direction is connected with the middle position of the upper left side of the movable base through a bolt.
Preferably, the mixing shaft and the detachable mixing blade are movably arranged inside the mixing barrel.
Preferably, the connecting sleeve is in bolted connection with the outlet at the middle position of the lower right side of the mixing barrel transversely towards the left end.
Preferably, the impurity filtering element is a carbon fiber filter element with an inverted trapezoidal longitudinal section.
Preferably, the control switch and the hybrid motor are connected through a wire, the control switch is a KCD1 type switch, and the hybrid motor is a 775 type motor.
Compared with the prior art, the beneficial effects of the utility model are that:
1. the utility model discloses in, hybrid motor, adjust the seat, the setting of mixing shaft and removable mixing leaf is favorable to improving mixing efficiency, guarantees to mix the effect.
2. The utility model discloses in, the regulating tube, the bracing piece, the setting of square head bolt and removal handle are favorable to adjusting the position of adjusting the seat according to mixing the demand to guarantee to mix the effect.
3. The utility model discloses in, delivery pipe, clearance bottom, a back cover section of thick bamboo and the setting of straining miscellaneous core are favorable to straining miscellaneous to the culture solution that takes out, can guarantee to use the convenience, can guarantee to detect the accuracy simultaneously.
4. The utility model discloses in, the control tube, adapter sleeve and control flap's setting is favorable to controlling the outflow.
5. The utility model discloses in, the supporting leg with remove the setting of wheel, be favorable to removing the device, convenient to use.
6. The utility model discloses in, the adsorption plate, the setting of collecting box and handle is favorable to collecting the culture solution that drips, avoids environmental pollution.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic structural diagram of the detachable adjusting mixing blade structure of the present invention.
Fig. 3 is a schematic structural view of the detachable filter leading-out core structure of the present invention.
In the figure:
1. feeding the culture solution into a hopper; 2. a flow guide pipe; 3. a mixing barrel; 4. moving the base; 5. arranging pipes; 6. a mechanical valve; 7. supporting legs; 8. a moving wheel; 9. an adsorption sheet; 10. a collection box; 11. a handle; 12. a control switch; 13. a detachable adjusting mixing blade structure; 131. a hybrid motor; 132. an adjusting seat; 133. a mixing shaft; 134. the mixing leaf can be detached; 135. an adjusting tube; 136. a support bar; 137. a square head bolt; 138. moving the handle; 14. a detachable filter lead-out core structure; 141. a discharge pipe; 142. cleaning the bottom cover; 143. a bottom sealing cylinder; 144. filtering the impurity core; 145. a control tube; 146. connecting a sleeve; 147. and controlling the valve.
Detailed Description
The utility model is described in detail with reference to the accompanying drawings, as shown in fig. 1 and fig. 2, a multifunctional cell culture solution mixing device for cell engineering comprises a culture solution feeding hopper 1, a guide pipe 2, a mixing barrel 3, a moving machine base 4, a discharge pipe 5, a mechanical valve 6, a support leg 7, a moving wheel 8, an adsorption sheet 9, a collection box 10, a handle 11, a control switch 12, a detachable adjusting mixing blade structure 13 and a detachable filtering leading-out core structure 14, wherein the middle position of the lower part of the culture solution feeding hopper 1 is in threaded connection with the upper right end of the guide pipe 2; the left lower end of the draft tube 2 is in threaded connection with the inlet of the upper right side of the mixing barrel 3; the longitudinal bottom of the mixing barrel 3 is connected with the middle position of the upper part of the movable base 4 through a bolt; the upper part of the calandria 5 in the longitudinal direction penetrates through the middle position in the moving machine base 4 and is in threaded connection with the outlet at the middle position at the bottom of the mixing barrel 3; the mechanical valve 6 is in threaded connection with the middle position of the front part of the discharge pipe 5; the supporting legs 7 are respectively connected with the four corners of the bottom of the movable base 4 by bolts at one longitudinal end and the middle position of the upper part of the movable wheel 8 by bolts at the other end; the adsorption pieces 9 are respectively and longitudinally adsorbed at the right lower side of the supporting leg 7 arranged at the right side; the adsorption sheets 9 are respectively longitudinally bolted on the front part and the rear part of the left side of the collection box 10; a handle 11 is connected with the middle position of the right part of the collecting box 10 through a screw; the control switch 12 is transversely bolted at the middle position of the left side of the front part of the movable base 4; the detachable adjusting mixing blade structure 13 and the detachable filtering leading-out core structure 14 are respectively connected with the mixing barrel 3; the detachable adjusting mixing blade structure 13 comprises a mixing motor 131, an adjusting seat 132, a mixing shaft 133, a detachable mixing blade 134, an adjusting pipe 135, a supporting rod 136, a square-head bolt 137 and a moving handle 138, wherein the longitudinal lower end of the mixing motor 131 is connected to the middle position of the upper part of the adjusting seat 132 through a bolt; the upper end of the mixing shaft 133 in the longitudinal direction penetrates through the middle position inside the adjusting seat 132; the coupling at the longitudinal upper end of the mixing shaft 133 is connected to the lower end of the output shaft of the mixing motor 131; the detachable mixing blade 134 is sleeved on the lower part of the outer wall of the mixing shaft 133 from top to bottom in sequence; the transverse left end of the adjusting seat 132 is connected with the middle position of the right end of the adjusting pipe 135 through a bolt; the adjusting pipe 135 is sleeved on the outer wall of the supporting rod 136 and is fastened and connected through a square-head bolt 137; remove 138 right-hand member bolted connection of handle in the left side intermediate position of control tube 135, with the culture solution through the culture solution advance fill 1 through leading-in mixing drum 3 inside of honeycomb duct 2, start control switch 12, it is rotatory to make hybrid motor 131 drive mixing shaft 133 and removable mixing leaf 134, can carry out the mixing operation, according to the mixing demand, square head bolt 137 can relax, handheld removal handle 138 removes and adjusts control tube 135 and moves at bracing piece 136 outer wall, can change the position of adjusting seat 132, can be convenient for carry out the mixing operation, guarantee mixing efficiency.
In this embodiment, referring to fig. 3, the detachable filter and lead-out core structure 14 includes a discharge pipe 141, a cleaning bottom cover 142, a bottom sealing barrel 143, a filter core 144, a control pipe 145, a connecting sleeve 146 and a control valve 147, wherein the upper longitudinal end of the discharge pipe 141 is screwed to the outlet at the middle position of the lower end of the cleaning bottom cover 142; the cleaning bottom cover 142 is in threaded connection with the outlet at the lower end of the bottom sealing barrel 143; the impurity filtering core 144 is longitudinally arranged inside the bottom sealing barrel 143, and the lower part of the impurity filtering core is in screw connection with the cleaning bottom cover 142; one end of the control pipe 145 is in threaded connection with an inlet at the middle position of the top of the bottom sealing cylinder 143, and the other end of the control pipe 145 is in threaded connection with the right end of the discharge pipe 141; control valve 147 threaded connection the left downside intermediate position of control tube 145, when getting, can unscrew control valve 147 for the inside culture solution of blending tank 3 passes through adapter sleeve 146 and flows into inside the bottom sealing section of thick bamboo 143 via control tube 145, and filters miscellaneous through straining miscellaneous core 144, discharges through discharge pipe 141 at last, can place test tube or other household utensils inside collection box 10, can take the culture solution, avoids environmental pollution.
In this embodiment, specifically, two permanent magnet pieces are used as the adsorption piece 9.
In this embodiment, specifically, the mixing shaft 133 and the detachable mixing blade 134 are disposed in a bolt connection manner.
In this embodiment, specifically, the detachable mixing blade 134 is a plurality of stainless steel spiral blades.
In this embodiment, specifically, the lower end of the support rod 136 is bolted to the middle position of the upper left side of the moving base 4.
In this embodiment, specifically, the mixing shaft 133 and the detachable mixing blade 134 are movably disposed inside the mixing barrel 3.
In this embodiment, specifically, the transverse left end of the connecting sleeve 146 is bolted to the outlet at the middle position of the lower right side of the mixing barrel 3.
In this embodiment, specifically, the impurity filtering element 144 specifically adopts a carbon fiber filter element with an inverted trapezoidal longitudinal section.
In this embodiment, a wire is specifically arranged between the control switch 12 and the hybrid motor 131, the control switch 12 specifically adopts a KCD1 type switch, and the hybrid motor 131 specifically adopts a 775 type motor.
Principle of operation
In the utility model, the culture solution is guided into the mixing barrel 3 through the culture solution feeding hopper 1 and the guide pipe 2, the control switch 12 is started, the mixing motor 131 drives the mixing shaft 133 and the detachable mixing blade 134 to rotate, mixing work can be carried out, the square head bolt 137 can be loosened according to mixing requirements, the adjusting pipe 135 is moved to move on the outer wall of the support rod 136 by the hand-held moving handle 138, the position of the adjusting seat 132 can be changed, mixing work can be conveniently carried out, mixing efficiency is ensured, when in use, the control valve 147 can be unscrewed, the culture solution in the mixing barrel 3 flows into the bottom sealing barrel 143 through the control pipe 145 by the connecting sleeve 146, impurities are filtered by the impurity filtering core 144, finally, the discharge pipe is discharged by the discharge pipe 141, a test tube or other utensils can be placed in the collecting box 10, the culture solution can be taken, environmental pollution is avoided, the device can be moved by the moving wheel 8, the operation is convenient.
Utilize technical scheme, or technical personnel in the field are in the utility model discloses under technical scheme's the inspiration, design similar technical scheme, and reach above-mentioned technological effect, all fall into the utility model discloses a protection scope.

Claims (9)

1. The cell culture solution mixing device for the multifunctional cell engineering is characterized by comprising a culture solution feeding hopper (1), a guide pipe (2), a mixing barrel (3), a moving base (4), a discharge pipe (5), a mechanical valve (6), a supporting leg (7), a moving wheel (8), an adsorption sheet (9), a collection box (10), a lifting handle (11), a control switch (12), a detachable adjusting mixing leaf structure (13) and a detachable filtering leading-out core structure (14), wherein the middle position of the lower part of the culture solution feeding hopper (1) is in threaded connection with the upper right end of the guide pipe (2); the left lower end of the draft tube (2) is in threaded connection with an inlet at the upper right side of the mixing barrel (3); the longitudinal bottom of the mixing barrel (3) is connected with the middle position of the upper part of the movable base (4) through a bolt; the upper part of the calandria (5) in the longitudinal direction penetrates through the middle position in the moving base (4) and is in threaded connection with an outlet at the middle position at the bottom of the mixing barrel (3); the mechanical valve (6) is in threaded connection with the middle position of the front part of the discharge pipe (5); the supporting legs (7) are respectively connected to the four corners of the bottom of the movable base (4) through bolts at one longitudinal end, and are connected to the middle position of the upper part of the movable wheel (8) through bolts at the other end; the adsorption pieces (9) are respectively and longitudinally adsorbed at the right lower side of the support leg (7) arranged at the right side; the adsorption pieces (9) are respectively and longitudinally connected with the front part and the rear part of the left side of the collection box (10) through bolts; a handle (11) is connected with the middle position of the right part of the collecting box (10) by a screw; the control switch (12) is transversely bolted at the middle position of the left side of the front part of the movable base (4); the detachable adjusting mixing blade structure (13) and the detachable filtering leading-out core structure (14) are respectively connected with the mixing barrel (3); the detachable adjusting mixing blade structure (13) comprises a mixing motor (131), an adjusting seat (132), a mixing shaft (133), a detachable mixing blade (134), an adjusting pipe (135), a supporting rod (136), a square-head bolt (137) and a moving handle (138), wherein the longitudinal lower end of the mixing motor (131) is in bolted connection with the middle position of the upper part of the adjusting seat (132); the upper end of the mixing shaft (133) in the longitudinal direction penetrates through the middle position inside the adjusting seat (132); the coupling at the longitudinal upper end of the mixing shaft (133) is connected to the lower end of the output shaft of the mixing motor (131); the detachable mixing blade (134) is sleeved on the lower part of the outer wall of the mixing shaft (133) from top to bottom in sequence; the transverse left end of the adjusting seat (132) is connected with the middle position of the right end of the adjusting pipe (135) through a bolt; the adjusting pipe (135) is sleeved on the outer wall of the supporting rod (136) and is fixedly connected and arranged through a square-head bolt (137); the right end of the movable handle (138) is connected with the middle position of the left side of the adjusting pipe (135) through a bolt.
2. The multifunctional cell culture solution mixing device for cell engineering as claimed in claim 1, wherein the removable filter cartridge outlet structure (14) comprises a drain pipe (141), a cleaning bottom cover (142), a bottom sealing barrel (143), a filter cartridge (144), a control pipe (145), a connecting sleeve (146) and a control valve (147), wherein the upper longitudinal end of the drain pipe (141) is screwed at the outlet of the middle position of the lower end of the cleaning bottom cover (142); the cleaning bottom cover (142) is in threaded connection with an outlet at the lower end of the bottom sealing barrel (143); the impurity filtering core (144) is longitudinally arranged inside the bottom sealing barrel (143), and the lower part of the impurity filtering core is in screw connection with the cleaning bottom cover (142); one end of the control pipe (145) is in threaded connection with an inlet at the middle position of the top of the bottom sealing cylinder (143), and the other end of the control pipe is in threaded connection with the right end of the discharge pipe (141); the control valve (147) is in threaded connection with the middle position of the lower left side of the control pipe (145).
3. The multifunctional cell culture solution mixing device for cell engineering according to claim 1, wherein the adsorption sheet (9) comprises two permanent magnet sheets.
4. The multifunctional cell culture solution mixing device for cell engineering according to claim 1, wherein the mixing shaft (133) and the detachable mixing blade (134) are bolted together.
5. The multifunctional cell culture solution mixing device for cell engineering according to claim 1, wherein the detachable mixing blade (134) is a plurality of stainless steel spiral blades.
6. The multifunctional cell culture solution mixing device for cell engineering according to claim 1, wherein the lower longitudinal end of the support rod (136) is bolted to the middle position of the upper left side of the mobile machine base (4).
7. The multifunctional cell culture solution mixing device for cell engineering according to claim 1, wherein the mixing shaft (133) and the detachable mixing blade (134) are movably disposed inside the mixing barrel (3).
8. The multifunctional cell culture solution mixing device for cell engineering according to claim 2, wherein the connecting sleeve (146) is bolted at the outlet at the middle position of the lower right side of the mixing barrel (3) at the transverse left end.
9. The multifunctional cell culture solution mixing device for cell engineering according to claim 2, wherein the impurity filtering element (144) is a carbon fiber filter element having an inverted trapezoidal longitudinal section.
CN201921548241.4U 2019-09-18 2019-09-18 Multi-functional cell culture liquid mixing arrangement for cell engineering Expired - Fee Related CN210620842U (en)

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Application Number Priority Date Filing Date Title
CN201921548241.4U CN210620842U (en) 2019-09-18 2019-09-18 Multi-functional cell culture liquid mixing arrangement for cell engineering

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Application Number Priority Date Filing Date Title
CN201921548241.4U CN210620842U (en) 2019-09-18 2019-09-18 Multi-functional cell culture liquid mixing arrangement for cell engineering

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112680400A (en) * 2020-12-15 2021-04-20 五河县康爱生物科技有限公司 Culture method of cell culture solution based on fetal calf serum
CN114813299A (en) * 2022-05-10 2022-07-29 无锡市第五人民医院 Medicine sensitive detection sample sampling pretreatment mixing arrangement

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112680400A (en) * 2020-12-15 2021-04-20 五河县康爱生物科技有限公司 Culture method of cell culture solution based on fetal calf serum
CN114813299A (en) * 2022-05-10 2022-07-29 无锡市第五人民医院 Medicine sensitive detection sample sampling pretreatment mixing arrangement

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Granted publication date: 20200526

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