CN210604664U

CN210604664U - Antibody concentration device

Info

Publication number: CN210604664U
Application number: CN201920893450.6U
Authority: CN
Inventors: 陈莉; 鲍诗卉; 王燕珠; 许咏梅
Original assignee: Wuhan Boster Bioengineering Co ltd
Current assignee: Wuhan Boster Bioengineering Co ltd
Priority date: 2019-06-13
Filing date: 2019-06-13
Publication date: 2020-05-22
Anticipated expiration: 2029-06-13

Abstract

The utility model relates to an antibody concentration device, which comprises a box body, a refrigeration mechanism, a net rack and a liquid collecting box, wherein the front side of the box body is opened, and the opening of the box body is provided with a box door which can open or close the opening; the interior of the box body is vertically provided with a partition board along the left and right directions, so that the interior of the box body is divided into a concentration chamber and a refrigeration chamber which are independent from each other; the refrigerating mechanism comprises a compressor and a cooler, the cooler is arranged in the refrigerating chamber, and the compressor is positioned outside the box body; the net rack is horizontally arranged in the concentration chamber, and two ends of the net rack are respectively connected with the inner side wall of the box body; the upper part of the liquid collecting box is provided with an opening, and the liquid collecting box is detachably arranged in the concentration chamber and is positioned below the net rack. The utility model discloses an antibody concentration device has improved the concentrated efficiency of antibody to make the recovery of polyethylene glycol 20000 more simple and convenient.

Description

Antibody concentration device

Technical Field

The utility model relates to an antibody preparation equipment technical field, concretely relates to antibody enrichment facility.

Background

Antibodies are a class of immunoglobulins that specifically bind to antigens and have a wide range of applications in medical practice, such as in the prevention, diagnosis and treatment of disease. At present, in the use process of the antibody, certain requirements on the concentration of the antibody are required, so the antibody needs to be concentrated after being purified on a purification column.

The principle of antibody concentration is as follows: the antibody solution to be concentrated is placed in a dialysis bag, the surface of the dialysis bag is fully paved with polyethylene glycol 20000, the polyethylene glycol 20000 is white hard waxy solid or thin sheet, the water absorption characteristic of the polyethylene glycol 20000 enables the water in the dialysis bag to be reduced, the antibody solution is concentrated, meanwhile, the polyethylene glycol 20000 becomes viscous liquid after water absorption, and the process needs to be carried out at 4 ℃ in order to keep the activity of the antibody. This process does not have special device now, the dialysis bag that will be equipped with the antibody solution of treating concentration is generally put in the box, polyethylene glycol 20000 is paved on the surface, then place in the refrigerator, concentrate, at the concentrated in-process of antibody, polyethylene glycol 20000 is because absorbed moisture becomes viscous liquid gradually, it piles up on the dialysis bag next door, lead to the hygroscopicity of other polyethylene glycol 20000 to reduce gradually, after concentrated completion, need take out the liquid polyethylene glycol 20000 recovery of thick form with the dialysis bag in the box and recycle, the dialysis bag after taking out needs to wash, the polyethylene glycol 20000 on its surface is directly washed away can't be retrieved.

SUMMERY OF THE UTILITY MODEL

The utility model provides a simple and efficient antibody concentration device for solving the problems.

The utility model provides an above-mentioned technical problem's technical scheme as follows: an antibody concentration device comprises a box body, a refrigeration mechanism, a net rack and a liquid collecting box, wherein the front side of the box body is provided with an opening, and a box door capable of opening or closing the opening is arranged at the opening of the box body; a partition plate is vertically arranged in the box body along the left-right direction, the interior of the box body is divided into a concentration chamber and a refrigeration chamber which are independent from each other in the front-back direction, the refrigeration mechanism comprises a compressor and a cooler, the cooler is arranged in the refrigeration chamber, and the compressor is positioned outside the box body; the net rack is horizontally arranged in the concentration chamber, and two ends of the net rack are respectively connected with the inner side wall of the box body; the liquid collecting box is provided with an opening above, and the liquid collecting box is detachably arranged in the concentration chamber and is positioned below the net rack.

The utility model has the advantages that: place the dialysis bag that will be equipped with the antibody solution of treating concentration on the rack, and the surface covering at the dialysis bag has slice polyethylene glycol 20000, refrigerating plant makes to keep at 4 ℃ in the concentration room, the continuous absorption of polyethylene glycol 20000 water in the dialysis bag becomes thick liquid, thick liquid drips to below collection liquid box through the mesh on the rack, make the polyethylene glycol 20000 that covers on the dialysis bag surface keep good hydroscopicity throughout, after the concentration, take out the collection liquid box and retrieve the polyethylene glycol 20000 of the mucus state after absorbing water of wherein collecting, cyclic utilization after the stoving, this antibody concentration device has improved the concentrated efficiency of antibody, and make the recovery of polyethylene glycol more simple and convenient.

On the basis of the technical scheme, the utility model discloses can also do following improvement.

Further, the collection liquid box is two, two the equal detachable setting of collection liquid box is in the concentration chamber, and the spaced setting is in from top to bottom the rack below.

Adopt the beneficial effect of above-mentioned further scheme to be full of mucus form polyethylene glycol 20000 among them collection liquid box and need dismantle from the concentration room when recovery processing, antibody concentration can continue to carry out in the device to another collection liquid box, and the device can work incessantly in succession.

Further, the net rack is detachably arranged in the concentration chamber.

Adopt above-mentioned further scheme's beneficial effect to use can pull down the rack after a period and wash thoroughly.

Further, the left and right side wall outside of collection liquid box is equipped with the draw runner along the fore-and-aft direction, be equipped with on the inside left and right sides lateral wall of concentrate room with draw runner assorted spout, draw runner slidable sets up in the spout.

The beneficial effects of adopting above-mentioned further scheme are that the cooperation of draw runner and spout makes the installation and the dismantlement that collect the liquid box can be convenient, simple structure, convenient to use.

Further, antibody enrichment facility still includes wiper mechanism, wiper mechanism sets up in the concentration chamber and be located the top of rack, in order to right the rack washs.

The beneficial effect of adopting above-mentioned further scheme is that after the antibody concentration is accomplished, still remain on dialysis bag surface and have mucus polyethylene glycol 20000, and belt cleaning device can wash the remaining mucus on dialysis bag surface, and the polyethylene glycol mucus after the washing falls into the collection liquid box, has improved the recovery efficiency of polyethylene glycol.

Further, wiper mechanism includes water tank, many outlet pipes and shower nozzle, the water tank level sets up, its inside cavity and upper end opening, the lower extreme interval of water tank is equipped with a plurality ofly and the apopore of many outlet pipes one-to-ones, the equal vertical setting of outlet pipe is in the below of water tank and its upper end respectively with correspond the apopore intercommunication, it is a plurality of the shower nozzle is with many the setting of outlet pipe one-to-one is in the below of outlet pipe and with the outlet pipe intercommunication, every all be equipped with the valve on the outlet pipe.

The beneficial effect who adopts above-mentioned further scheme is that this wiper mechanism simple structure, and can wash the dialysis bag according to the quantity of dialysis bag and the shower nozzle of opening its top of position selectivity.

Drawings

Fig. 1 is a cross-sectional view of embodiment 1 of the present invention in the left-right direction;

fig. 2 is a front-rear sectional view of embodiment 1 of the present invention;

fig. 3 is a schematic structural view of a net rack in embodiment 1 of the present invention;

fig. 4 is a cross-sectional view of embodiment 2 of the present invention in the left-right direction;

fig. 5 is a front-rear sectional view of embodiment 2 of the present invention;

fig. 6 is a cross-sectional view of embodiment 3 of the present invention in the left-right direction;

fig. 7 is a cross-sectional view of embodiment 3 of the present invention in the front-rear direction.

In the drawings, the components represented by the respective reference numerals are listed below:

10. the refrigerator comprises a box body, 11, a box door, 12, a partition board, 13, a concentration chamber, 14, a refrigeration chamber, 15, a sliding groove, 16, a groove, 21, a compressor, 22, a cooler, 30, a net rack, 40, a liquid collecting box, 41, a sliding strip, 51, a water tank, 52, a water outlet pipe, 53, a spray head, 54, a valve, 60 and a rear box.

Detailed Description

The principles and features of the present invention are described below in conjunction with the following drawings, the examples given are only intended to illustrate the present invention and are not intended to limit the scope of the present invention.

Example 1

As shown in fig. 1-3, the utility model provides an antibody concentration device, which comprises a box body 10, a refrigeration mechanism, a net frame 30 and a liquid collecting box 40, wherein the front side of the box body 10 is opened and the opening of the box body is provided with a box door 11 which can open or close the opening; a partition plate 12 is vertically arranged in the box body 10 along the left-right direction, and divides the interior of the box body 10 into a concentration chamber 13 and a refrigeration chamber 14 which are independent from each other in the front and the back; the net rack 30 is horizontally arranged in the concentration chamber 13, two ends of the net rack 30 are respectively connected with the inner side wall of the box body 10 and used for placing a dialysis bag filled with the antibody to be concentrated, and the size of the mesh on the net rack 30 can be designed according to the size of the dialysis bag and the size of the sheet-shaped polyethylene glycol 20000, so that the sheet-shaped polyethylene glycol 20000 covered on the dialysis bag can not fall off from the mesh; the upper part of the liquid collecting box 40 is opened, and the liquid collecting box is detachably arranged in the concentrating chamber 13 and is positioned below the net rack 30.

The refrigeration mechanism includes a compressor 21 and a cooler 22, the cooler 22 is disposed in the refrigeration chamber 14, the compressor 21 is disposed outside the casing 10, the device further includes a rear case 60, the rear case 60 and a rear side wall of the casing 10 form a compressor 21 chamber, the compressor 21 is disposed in the compressor 21 chamber, a connection relationship between the compressor 21 and the cooler 22 is a conventional technique, a high-temperature and high-pressure refrigerant gas compressed by the compressor 21 is cooled to a high-pressure liquid refrigerant by a condenser (not shown), and then is reduced in pressure by a pressure reducing device (usually, a capillary tube) (not shown) to become a low-pressure two-phase refrigerant, and the low-pressure two-phase refrigerant cools the condensation chamber 13 of the antibody condensation device by an evaporator of the cooler 22 to become a low-pressure gas refrigerant, and returns to the compressor 21, and the refrigeration cycle is repeated.

Place the dialysis bag that is equipped with the antibody solution of treating the concentration on rack 30, and the surface covering at the dialysis bag has slice polyethylene glycol 20000, refrigerating plant makes and keeps at 4 ℃ in the concentration room 13, the continuous absorption of polyethylene glycol 20000 water in the dialysis bag becomes thick liquid, thick liquid drips to the below collection liquid box 40 through the mesh on rack 30, make the polyethylene glycol 20000 that covers on dialysis bag surface keep good hydroscopicity throughout, after the concentration, take out the collection liquid box 40 and retrieve the polyethylene glycol 20000 of the mucus state after absorbing water of collecting wherein, cyclic utilization after the stoving, this antibody concentration device has improved the concentrated efficiency of antibody, and make the recovery of polyethylene glycol more simple and convenient.

Preferably, the net rack 30 is detachably disposed in the concentration chamber 13, grooves 16 are disposed on the left and right side walls of the concentration chamber 13 along the front-back direction, the left and right ends of the net rack 30 are clamped in the grooves 16 and can move back and forth along the grooves 16 to be placed in or taken out of the concentration chamber 13, and after the antibody concentration device is used for a period of time, the net rack 30 can be detached to be thoroughly cleaned.

Preferably, sliding strips 41 are arranged on the outer sides of the left and right side walls of the liquid collecting box 40 along the front-back direction, sliding grooves 15 matched with the sliding strips 41 are arranged on the left and right side walls inside the concentrating chamber 13, the sliding strips 41 are slidably arranged in the sliding grooves 15, and the liquid collecting box 40 can move back and forth to be placed in or pulled out of the concentrating chamber 13.

The cooperation of the sliding strip 41 and the sliding groove 15 enables the liquid collecting box 40 to be conveniently mounted and dismounted, and the liquid collecting box is simple in structure and convenient to use.

Example 2

As shown in fig. 4 to 5, unlike the embodiment 1, two liquid collecting boxes 40 are provided, and both the two liquid collecting boxes 40 are detachably provided in the concentrating chamber 13 and are vertically spaced below the rack 30.

When the mucus-like polyethylene glycol 20000 in one of the liquid collecting boxes 40 is filled and needs to be removed from the concentrating chamber 13 for recovery processing, the other liquid collecting box 40 can ensure that the antibody concentration continues in the device, and the device can continuously and uninterruptedly work.

Example 3

As shown in fig. 6 to 7, unlike in example 1, the antibody concentration device further includes a cleaning mechanism provided in the concentration chamber 13 above the rack 30 to clean the rack 30. After the antibody concentration is finished, mucus-like polyethylene glycol 20000 is remained on the surface of the dialysis bag, the cleaning device can clean the mucus remained on the surface of the dialysis bag, and the cleaned polyethylene glycol mucus falls into the liquid collecting box 40, so that the recovery efficiency of polyethylene glycol is improved.

Preferably, the cleaning mechanism includes a water tank 51, a plurality of outlet pipes 52 and spray heads 53, the water tank 51 is horizontally arranged, the interior of the water tank 51 is hollow, the upper part of the water tank is opened, a plurality of water outlet holes corresponding to the plurality of outlet pipes 52 are arranged at intervals at the lower end of the water tank 51, the outlet pipes 52 are vertically arranged below the water tank 51, the upper ends of the outlet pipes are respectively communicated with the corresponding water outlet holes one by one, the spray heads 53 and the plurality of outlet pipes 52 are arranged below the outlet pipes 52 in one-to-one correspondence, the spray heads 52 are communicated with the outlet pipes 52, and each outlet pipe 52 is provided with a valve 54 to open or close the spray heads. The sprayer 53 selects a sprayer which does not contain a pressurizing device in the market, and simultaneously, the height of the water level in the water tank 51 is adjusted according to the water pressure, so that the dialysis bag is prevented from being broken by too high water pressure caused by too high water level. The water tank 51 is added with water from an opening above the water tank 51 in advance, the water is pre-cooled in the concentration chamber 13, after the antibody concentration is finished, the valve 54 above the position of the dialysis bag is opened, the dialysis bag is cleaned, the cleaning mechanism is simple in structure, and the spray nozzles 53 above the dialysis bag can be selectively opened according to the number and the position of the dialysis bag to clean the dialysis bag.

The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.

Claims

1. An antibody concentration device is characterized by comprising a box body (10), a refrigeration mechanism, a net rack (30) and a liquid collecting box (40);

the front side of the box body (10) is provided with an opening, and the opening is provided with a box door (11) capable of opening or closing the opening;

a partition plate (12) is vertically arranged in the box body (10) along the left-right direction, and divides the interior of the box body (10) into a concentration chamber (13) and a refrigeration chamber (14) which are independent from each other in the front-back direction;

the refrigerating mechanism comprises a compressor (21) and a cooler (22), the cooler (22) is arranged in the refrigerating chamber (14), and the compressor (21) is positioned outside the box body (10);

the net rack (30) is horizontally arranged in the concentration chamber (13), and two ends of the net rack are respectively connected with the inner side wall of the box body (10);

the upper part of the liquid collecting box (40) is opened, and the liquid collecting box is detachably arranged in the concentration chamber (13) and is positioned below the net rack (30).

2. The antibody concentrating apparatus according to claim 1, wherein the number of the liquid collecting boxes (40) is two, and both the liquid collecting boxes (40) are detachably disposed in the concentrating chamber (13) and are disposed below the rack (30) at intervals.

3. An antibody concentrating device according to claim 1 or 2, wherein the rack (30) is removably arranged in the concentrating compartment (13).

4. An antibody concentrating device according to claim 1 or 2, wherein the collecting box (40) is provided with slide bars (41) at the outer sides of the left and right side walls along the front-back direction, the concentrating chamber (13) is provided with slide grooves (15) matched with the slide bars (41) at the inner left and right side walls, and the slide bars (41) are slidably arranged in the slide grooves (15).

5. An antibody concentrating apparatus according to claim 4, further comprising a cleaning mechanism disposed within the concentrating compartment (13) above the rack (30) to clean the rack (30).

6. The antibody concentrating device according to claim 5, wherein the cleaning mechanism comprises a water tank (51), a plurality of water outlet pipes (52) and spray heads (53), the water tank (51) is horizontally arranged, the interior of the water tank (51) is hollow, the upper end of the water tank is open, a plurality of water outlet holes corresponding to the plurality of water outlet pipes (52) are formed in the lower end of the water tank (51) at intervals, the water outlet pipes (52) are vertically arranged below the water tank (51), the upper ends of the water outlet pipes are respectively communicated with the corresponding water outlet holes, the spray heads (53) and the plurality of water outlet pipes (52) are correspondingly arranged below the water outlet pipes (52) and are communicated with the water outlet pipes (52), and each water outlet pipe (52) is provided with a valve (54).