CN210604381U

CN210604381U - Bacteria turbidimeter

Info

Publication number: CN210604381U
Application number: CN201921301534.2U
Authority: CN
Inventors: 赵亚南; 任丽; 方辰龙
Original assignee: Zhengzhou Zhenghe Medical Instruments Co ltd
Current assignee: Zhengzhou Zhenghe Medical Instruments Co ltd
Priority date: 2019-08-12
Filing date: 2019-08-12
Publication date: 2020-05-22
Anticipated expiration: 2029-08-12

Abstract

The utility model discloses a bacteria turbidity meter, which comprises an instrument main body, a display unit, a measuring unit and a control operation unit; the display unit is arranged on the surface of the instrument main body and is electrically connected with the control operation unit arranged in the instrument main body; the measuring unit comprises a light emitting device, a light passage chamber, a container positioning hole, a light receiver and a scattered light recovery device, and scattered light is compensated into the light receiver by adding the scattered light recovery device, so that OD value measuring errors caused by scattering are reduced as much as possible. And a plurality of measuring units are arranged to realize batch detection. Simple structure is reliable, has improved detection efficiency and detection precision.

Description

Bacteria turbidimeter

Technical Field

The utility model relates to a liquid measuring instrument field, concretely relates to bacterium turbidity appearance.

Background

A bacteria turbidimeter is a device used to determine the concentration of bacteria in a suspension of a strain to be tested. The existing method for measuring bacterial turbidity is to make light with a certain wavelength pass through a plurality of bacterial suspensions with known concentrations, measure the OD value (OD is an abbreviation of optical density, which indicates the optical density absorbed by the detected object and is a special term in the detection method, the detection unit is represented by OD (lg (1/trans), wherein trans is the light transmission value of the detected object, and is also called the light transmission rate.), draw corresponding curves of different concentrations and corresponding OD values, then measure the OD value of bacterial suspension with unknown concentration, and finally substitute the OD value into the curves to obtain the estimated OD value. However, when light passes through a bacterial suspension, in addition to attenuation of the light by absorption, other factors such as scattering also cause attenuation of the light. And the scattering coefficients of bacterial suspensions of different concentrations are different, and the influence of scattering is difficult to eliminate through comparison.

Therefore, in order to solve the above problems, there is a need for a bacteria turbidity meter capable of minimizing the influence on the measurement of an OD value due to the difference in scattering coefficients of solutions, thereby making the measured bacteria concentration more accurate.

SUMMERY OF THE UTILITY MODEL

In view of the above, the present invention is directed to overcoming the drawbacks of the prior art and providing a bacteria turbidity meter.

The utility model discloses a bacteria turbidimeter, which comprises an instrument main body, a display unit, a measuring unit and a control operation unit; the display unit is arranged on the surface of the instrument main body and is electrically connected with the control operation unit arranged in the instrument main body; the measuring unit comprises a light emitting device, a light passage chamber, a container positioning hole, a light receiver and a scattered light recovery device; a transverse round hole is formed in the instrument main body to serve as the light passage chamber; one end of the light passage chamber is provided with the light-emitting device, the other end of the light passage chamber is provided with a scattered light recovery device and a light receiver, and the light receiver is arranged at the focus of the scattered light recovery device; the optical receiver is electrically connected with the control arithmetic unit; the container positioning hole is arranged and penetrates through the top of the instrument main body to be connected with the light passage chamber. The scattered light recovery device can collect scattered light influencing the OD value of the bacterial suspension onto the light receiver as much as possible, so that the measurement result is more accurate.

Further, the scattered light recovery device comprises a concave mirror and a convex lens; the concave mirror surface faces the light-emitting device; the probe of the light receiver is spherical and is arranged at the focus of the concave mirror; the convex lens and the concave mirror are arranged between the container positioning hole and the light receiver in parallel, and the focus of the convex lens is positioned on the central axis of the container positioning hole. The area of the convex lens and the concave mirror is equal to the cross-sectional area of the light path chamber. The light passes through the bacterial suspension and is scattered, and because the bacterial suspension container is positioned on the focus of the convex lens, the convex lens can convert scattered light into parallel light as much as possible, and then the concave mirror collects the parallel light to the probe of the light receiver positioned on the focus of the concave mirror. In addition, the convex lens also has a small influence on the measurement of the OD value due to light absorption and scattering, but the influence on the measurement of the OD value due to the convex lens is not changed, so that the influence on the OD value due to the convex lens can be eliminated to the maximum extent by making a curve contrast.

Furthermore, the probe of the light receiver, the center of the convex lens and the light-emitting device are positioned on the same straight line, and the central axis of the container positioning hole is perpendicular to and intersected with the straight line. Mounting the points or devices on the same line ensures that the main direct light can reach the probe of the optical receiver accurately.

Further, the inner wall of the light passage chamber is smooth. In addition to the light reaching the light receiver through the above process, a part of the light does not satisfy the above light path condition, and the part of the light is projected onto the inner wall of the light path chamber, so that the inner wall of the light path chamber is designed to be a non-smooth light absorption surface, and errors caused by the part of the uncertain light can be effectively avoided.

Further, a plurality of measurement units are arranged in the instrument body side by side. The detection efficiency can be improved, the OD values of a plurality of groups of bacterial suspensions with different concentrations can be directly detected, and a comparison curve can be conveniently made.

The utility model has the advantages that: the utility model discloses a bacterium turbidity meter compensates the scattered light to light receiver in through increasing a scattered light recovery unit, has reduced the OD value measuring error because the scattering brings as far as possible. And a plurality of measuring units are arranged to realize batch detection. Simple structure is reliable, has improved detection efficiency and detection precision.

Drawings

The invention will be further described with reference to the following figures and examples:

fig. 1 is a schematic structural view of the present invention;

fig. 2 is a schematic diagram of the transverse cross-section structure of the present invention;

fig. 3 is a schematic longitudinal sectional structure of the present invention;

FIG. 4 is a schematic diagram of the connection of the control and operation module of the present invention;

fig. 5 is a schematic view of the measurement light path of the present invention.

Detailed Description

Fig. 1 is a schematic structural diagram of the present invention, and as shown in the figure, the bacteria turbidimeter in this embodiment. Comprises an instrument body 1, a display unit 2, a measuring unit 3 and a control arithmetic unit 4; the display unit 2 is arranged on the surface of the instrument body 1 and is electrically connected with a control operation unit 4 arranged in the instrument body 1; the measuring unit 3 includes a light emitting device 31, a light passage chamber 32, a container positioning hole 33, a light receiver 34, and a scattered light recovery device 35; a transverse circular hole is formed in the instrument body 1 as a light passage chamber 32 (transverse direction is a direction of a line parallel to the bottom surface of the instrument and emitted from the display screen end of the instrument); a light emitting device 31 (an LED light source not including ultraviolet wavelengths in this embodiment) is provided at one end of the light passage chamber 32, and a scattered light recovery device 35 and a light receiver 34 are provided at the other end; the light receiver 34 is electrically connected with the control arithmetic unit 4; the container positioning hole 33 is provided and penetrates the top of the instrument body 1 to connect the light passage chamber 32. The scattered light recovery device 35 can collect scattered light which influences the OD value of the bacterial suspension onto the light receiver 34 as much as possible, so that the measurement result is more accurate. At the same time, the optical receiver 34 transmits the detected optical density signal to the control arithmetic unit 4, and the measured OD value is displayed on the display unit 2.

The scattered light recovery device includes a concave mirror 351 and a convex lens 352; the concave mirror 351 is mirror-faced to the light-emitting device 31; the probe of the light receiver 34 is spherical and is disposed at the focal point of the concave mirror 351; the convex lens 352 and the concave mirror 351 are installed in parallel between the container positioning hole 33 and the light receiver 34, and the focal point of the convex lens 352 is located on the central axis of the container positioning hole 33. The areas of the convex lens 352 and the concave mirror 351 are equal to the cross-sectional area of the light passage chamber 32. The light passes through the bacterial suspension and is scattered, and since the bacterial suspension container is located at the focal point of the convex lens 352, the convex lens 352 changes the scattered light into parallel light as much as possible, and then the concave mirror 351 collects the parallel light to the probe of the light receiver located at the focal point of the concave mirror. Through the process, partial scattered light is recovered, and the accuracy of measuring the OD value of the bacterial suspension is improved. In addition, the convex lens also has a small influence on the measurement of the OD value due to light absorption and scattering, but the influence on the measurement of the OD value due to the convex lens is not changed, so that the influence on the OD value due to the convex lens can be eliminated to the maximum extent by making a curve contrast.

The probe of the light receiver 34, the center of the convex lens 352, and the light emitting device 31 are located on the same straight line, and the central axis of the container positioning hole 33 is perpendicular to and intersects with the straight line. Mounting the points or devices on the same line ensures that the main direct light can reach the probe of the optical receiver accurately.

The inner wall of the light passage chamber 32 is smooth. In addition to the light reaching the light receiver 34 through the above process, a part of the light does not satisfy the above optical path condition, and the part of the light is projected onto the inner wall of the light passage chamber 34, so that the inner wall of the light passage chamber 34 is designed as a non-smooth light absorbing surface, and the error caused by the part of the uncertain light can be effectively avoided.

A plurality of measurement units 3 are arranged side by side in the instrument main body 1. The detection efficiency can be improved, a plurality of groups of bacterial suspensions with known concentration can be directly detected, and the corresponding OD values can be directly read in the display unit 2, so that the corresponding curves of the suspension concentration and the OD values can be conveniently drawn.

Through the steps, the OD value measurement error caused by scattering is reduced as much as possible. And a plurality of measuring units are arranged to realize batch detection. Simple structure is reliable, has improved detection efficiency and detection precision.

Finally, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the present invention can be modified or replaced by other means without departing from the spirit and scope of the present invention, which should be construed as limited only by the appended claims.

Claims

1. A bacteria turbidimeter, characterized in that: comprises an instrument main body, a display unit, a measuring unit and a control operation unit; the display unit is arranged on the surface of the instrument main body and is electrically connected with the control operation unit arranged in the instrument main body; the measuring unit comprises a light emitting device, a light passage chamber, a container positioning hole, a light receiver and a scattered light recovery device; a transverse round hole is formed in the instrument main body to serve as the light path chamber, the light emitting device is arranged at one end of the light path chamber, the scattered light recovery device and the light receiver are arranged at the other end of the light path chamber, and the light receiver is arranged at the focus of the scattered light recovery device; the optical receiver is electrically connected with the control arithmetic unit; the container positioning hole is arranged and penetrates through the top of the instrument main body to be connected with the light passage chamber.

2. Bacteria nephelometer according to claim 1, characterized in that: the scattered light recovery device comprises a concave mirror and a convex lens; the concave mirror surface faces the light-emitting device; the probe of the light receiver is spherical and is arranged at the focus of the concave mirror; the convex lens and the concave mirror are arranged between the container positioning hole and the light receiver in parallel, and the focus of the convex lens is positioned on the central axis of the container positioning hole; the area of the convex lens and the concave mirror is equal to the cross-sectional area of the light path chamber.

3. Bacteria nephelometer according to claim 2, characterized in that: the probe of the light receiver, the center of the convex lens and the light-emitting device are positioned on the same straight line, and the central axis of the container positioning hole is perpendicular to and intersected with the straight line.

4. The bacteria turbidimeter of claim 1, wherein said light passage chamber has a non-smooth light absorbing surface on an inner wall thereof.

5. Bacteria nephelometer according to claim 1, characterized in that: a plurality of measuring units are arranged in the instrument main body side by side.