CN210572334U

CN210572334U - Chemiluminescent microfluidic chip

Info

Publication number: CN210572334U
Application number: CN201921549402.1U
Authority: CN
Inventors: 董丽静
Original assignee: Shenzhen Kerida Biotechnology Co Ltd
Current assignee: Shenzhen Kerida Biotechnology Co Ltd
Priority date: 2019-09-12
Filing date: 2019-09-12
Publication date: 2020-05-19
Anticipated expiration: 2029-09-12

Abstract

The utility model belongs to the technical field of medical instrument's technique and specifically relates to a chemiluminescence micro-fluidic chip is related to. The chip comprises a chip main body, a sample inlet channel, a sample filtering area and a composite area, wherein the sample inlet channel and the sample filtering area are arranged on the chip main body and are sequentially communicated, the composite area is used for carrying out immunoreaction and photometry, and an enzyme-labeled antibody is coated in the composite area; the chemiluminescent microfluidic chip also comprises a reagent storage area which is arranged on the chip main body and used for storing cleaning fluid, a luminescent substrate and an enzyme-labeled antibody, and a circulating extrusion area which is used for driving a sample to circularly flow in the composite area, wherein the head end and the tail end of the circulating extrusion area are respectively communicated with the head end and the tail end of the composite area, and the reagent storage area is communicated with the head end of the composite area. When detection is carried out, the sample is driven to circularly flow in the compound area by the circular extrusion area, so that all reactants in the compound area are fully contacted and completely reacted, and the accuracy of the analysis result of the chip is improved.

Description

Chemiluminescent microfluidic chip

Technical Field

The utility model relates to the technical field of medical equipment, more specifically say, relate to a chemiluminescence micro-fluidic chip.

Background

In Vitro Diagnosis (IVD) refers to taking a sample (blood, body fluid, tissue, etc.) from a human body and performing detection analysis to diagnose a disease, wherein corresponding instruments and reagents are required In the detection process. Chemiluminescence refers to the phenomenon in which chemical energy is converted into light energy by reaction intermediates, reaction products or an additional luminescent reagent in a chemical reaction process. Microfluidic chips, also known as Lab-on-a-chips, generally integrate basic operations such as sample preparation, reaction, separation, detection, etc. in biological, chemical, and medical analysis processes on one Chip to perform a system function.

In the reaction and analysis based on the microfluidic chip for in vitro diagnosis in the prior art, the basic process is to pre-store one or more reaction reagents in the chip or introduce one or more reaction reagents from the outside, then add a liquid sample to the chip, make the sample contact with the reagents according to a preset microchannel flow path and react, and read the result by an instrument or naked eyes.

In order to achieve the accuracy of the analysis result, it is necessary to ensure that the reaction is sufficient, but in practical cases, in the process of the reaction reagent or the sample entering the reaction region through the microchannel flow path, the reaction of the reagent or the substrate of the predetermined reaction is incomplete because the reaction reagent or the sample flows through the reaction region only once, the sample is too little to fill the predetermined region, or the contact is insufficient due to other reasons, so that the analysis result is inaccurate.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a chemiluminescence micro-fluidic chip aims at solving current chip testing process, and the sample is too few and can't fill the contact that predetermined area arouses inadequately for the reagent of predetermined reaction or the incomplete technical problem that leads to the analytical result inaccuracy of substrate reaction.

In order to solve the technical problem, the utility model provides a chemiluminescence micro-fluidic chip, include:

the chip comprises a chip main body, a sample inlet channel, a sample filtering area and a composite area, wherein the sample inlet channel and the sample filtering area are arranged on the chip main body and are sequentially communicated; the complex region is coated with an enzyme-labeled antibody;

the chemiluminescent microfluidic chip also comprises a reagent storage area which is arranged on the chip main body and used for storing cleaning fluid, a luminescent substrate and an enzyme-labeled antibody, and a circulating extrusion area which is used for driving a sample to circularly flow in the composite area, wherein the head end and the tail end of the circulating extrusion area are respectively communicated with the head end and the tail end of the composite area, and the reagent storage area is communicated with the head end of the composite area.

Optionally, the sample inlet channel is a channel formed by physically modifying the inner surface to change the linearity thereof, so that the sample can generate a capillary phenomenon at the sample inlet channel and automatically flow into the recombination zone through the sample filtration zone.

Optionally, the chemiluminescent microfluidic chip further comprises a driving channel disposed on the chip body, and a driving squeezing area for driving the sample to enter the recombination area after passing through the sample filtering area;

the head end and the tail end of the driving channel are respectively communicated with the driving extrusion area and the head end of the sample inlet channel.

Optionally, the chemiluminescent microfluidic chip further comprises a sample injection port disposed at the head end of the sample inlet channel, and a detachable gland is sealed on the sample injection port.

Optionally, the chemiluminescent microfluidic chip further comprises a sample degassing chamber arranged on the chip main body and used for removing gas in a sample, and the head end and the tail end of the sample degassing chamber are respectively communicated with the tail end of the sample filtering area and the head end of the composite area.

Optionally, the chemiluminescent microfluidic chip further comprises a waste liquid channel and a waste liquid pool arranged on the chip main body, and the head end and the tail end of the waste liquid channel are respectively communicated with the tail end of the composite region and the waste liquid pool.

Optionally, the chemiluminescent microfluidic chip further comprises a channel gate arranged on the chip body and used for preventing backflow, wherein the channel gate is arranged on the waste liquid channel and is positioned between the waste liquid pool and the tail end of the circular extrusion area.

Optionally, a sample filtering membrane is disposed in the sample filtering area, and a sample degassing membrane is disposed in the sample degassing chamber.

Optionally, the reagent storage area includes a storage pool containing a reagent pack, a valve plate for opening the reagent pack, and a reagent channel for communicating the storage pool with the head end of the recombination zone.

Optionally, the chip body of the chemiluminescent microfluidic chip comprises a substrate, and an upper membrane and a lower membrane which are respectively arranged on the upper surface and the lower surface of the substrate;

the substrate is provided with micropores, microchannels or microcavity to form the sample inlet channel, the sample filtering area, the composite area and the circular extrusion area with the upper membrane or the lower membrane.

The utility model provides a chemiluminescent microfluidic chip's beneficial effect lies in: compared with the prior art, the utility model discloses a chemiluminescent micro-fluidic chip is through setting up the circulation extrusion district that the head and the tail both ends communicate with the head and the tail both ends in complex area respectively in the chip main part for when examining, usable circulation extrusion district drive sample is at the internal circulation of complex area and flows, thereby makes contact fully and react completely between each reactant in the complex area, and then has improved the accuracy of chip analysis result.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the embodiments or the prior art descriptions will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive labor.

Fig. 1 is a schematic structural diagram of a chemiluminescent microfluidic chip according to an embodiment of the present invention;

fig. 2 is a schematic structural diagram of a circular extrusion region of a chemiluminescent microfluidic core according to one embodiment of the present invention;

fig. 3 is a schematic structural diagram of the channel gate of the chemiluminescent microfluidic core in cooperation with the ejection portion of an external instrument according to an embodiment of the present invention;

fig. 4 is a schematic view of a chip main structure of a chemiluminescent microfluidic chip according to an embodiment of the present invention.

In the figure: 101-sample entry channel; 102-a sample filtration zone; 103-a recombination zone; 1031-composite partition; 104-a reagent storage region; 1041-a storage partition; 1042 — a reagent channel; 10411-storage pool; 105-a circulating extrusion zone; 106-drive channel; 107-driving the extrusion zone; 108-sample injection port; 109-a gland; 110-positioning holes; 111-sample degassing chamber; 112-a waste channel; 113-waste liquid pool; 1051-a cyclic extrusion chamber; 1052-a circulation channel; 1-a substrate; 2, coating a film; 3-film laying; 4-the ejection part.

Detailed Description

Reference will now be made in detail to the embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The following embodiments with reference to the drawings are illustrative and intended to explain the present invention, and should not be construed as limiting the invention.

In the description of the present invention, it is to be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientation or positional relationship indicated in the drawings for convenience in describing the present invention and to simplify the description, but are not intended to indicate or imply that the device, element, or structure so referred to must have a particular orientation, be constructed and operated in a particular orientation, and are not to be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the present invention, "a plurality" means two or more unless specifically defined otherwise.

In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "communicating" are to be construed broadly, e.g., as meaning mechanically or electrically connected; the connection may be direct, indirect or internal, or may be a connection between two elements or an interaction relationship between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

It is also to be understood that the terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used in the specification and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise.

The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to the drawings of the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be embodied in other specific forms other than those described herein, and it will be apparent to those skilled in the art that the present invention may be practiced without departing from the spirit and scope of the invention.

As shown in fig. 1 to 4, the embodiment of the present invention provides a chemiluminescent microfluidic chip, which comprises a chip main body, and a sample inlet channel 101, a sample filtering area 102 and a composite area 103, which are arranged on the chip main body and are sequentially connected, wherein the composite area 103 is used for performing immunoreaction and photometry, and in addition, an enzyme-labeled antibody is coated inside the composite area 103. In this embodiment, the chemiluminescent microfluidic chip further comprises a reagent storage region 104 arranged on the chip main body and used for storing a cleaning solution, a luminescent substrate and an enzyme-labeled antibody, and a circulating extrusion region 105 used for driving a sample to circularly flow in the composite region 103, wherein the head end and the tail end of the circulating extrusion region 105 are respectively communicated with the head end and the tail end of the composite region 103, and the reagent storage region 104 is communicated with the head end of the composite region 103.

As described above, the embodiment of the utility model provides a chemiluminescent microfluidic chip is through set up the circulation extrusion district 105 of head and tail both ends respectively with the head and tail both ends intercommunication of complex region 103 in the chip main part for when examining, usable circulation extrusion district 105 drive sample is at complex region 103 inner loop flow, thereby makes contact fully and the reaction is complete between each reactant in the complex region 103, and then has improved the accuracy of chip analysis result.

In an application scenario, the complex region 103 may include a plurality of sequentially connected complex partitions 1031 with the same structure, in this embodiment, six complex partitions 1031 with the same structure are preferably disposed on the chip main body, and are coated with different enzyme-labeled antibodies to capture different antigens in a sample, thereby implementing multi-project joint inspection. Of course, in other embodiments of the present invention, the compound area 103 may have other configurations, which are not limited herein, according to the actual situation and the specific requirement.

In one application scenario, the sample inlet channel 101 is preferably a channel whose inner surface is physically modified to change its linear dimension such that the sample can be wicked in the sample inlet channel 101 to flow into the recombination zone 103 via the sample filtration zone 102. Of course, according to the actual situation and the specific requirement, in other embodiments of the present invention, the sample inlet channel 101 may also have other configurations, or the sample may be introduced into the recombination region 103 through the sample inlet channel 101 by vacuuming the sample inlet channel 101, which is not limited herein.

In this embodiment, the chemiluminescent microfluidic chip further includes a driving channel 106 disposed on the chip main body, and a driving squeezing area 107 for driving the sample to flow into the recombination area 103 after passing through the sample filtering area 102, and the head and the tail of the driving channel 106 are respectively communicated with the driving squeezing area 107 and the head of the sample inlet channel 101. Here, the driving squeezing area 107 may be squeezed by an external force provided by an external instrument adapted to the chemiluminescent microfluidic chip, where the external force may be provided by a syringe pump, a diaphragm pump, a peristaltic pump, or the like.

As shown in fig. 1, the chemiluminescent microfluidic chip further comprises a sample injection port 108 disposed at the head end of the sample inlet channel 101, wherein the sample injection port 108 is covered with a removable cover 109, and the cover 109 is used for sealing the main sample inlet 108. In this embodiment, a reliable seal can be achieved between the gland 109 and the sample injection port 108 by making one of them of a lower durometer material, such as rubber, and the other of a harder durometer material, such as plastic, in combination with a structural design.

As shown in fig. 1, in the present embodiment, the chip main body is further provided with a positioning hole 110, and the positioning hole 110 is used for the external instrument to position the chip main body.

As shown in fig. 1, in the present embodiment, the chemiluminescent microfluidic chip further includes a sample degassing chamber 111 disposed on the chip body for removing gas in the sample, and two ends of the sample degassing chamber 111 are respectively connected to the tail end of the sample filtering region 102 and the head end of the recombination region 103. Thus, the sample enters the sample filtering region 102 for filtering after passing through the sample inlet channel 101, and then enters the sample degassing chamber 111 for degassing. Specifically, the sample filtering area 102 is provided with a sample filtering membrane for filtering red blood cells in the whole blood sample, so that other components in the blood can smoothly pass through the sample filtering membrane, and the sample degassing chamber 111 is provided with a sample degassing membrane for removing gas in the sample by using a physical, chemical or biological method, so that the test result is more accurate.

As shown in fig. 1, in this embodiment, the chemiluminescent microfluidic chip further includes a waste liquid channel 112 and a waste liquid pool 113 disposed on the chip main body, and the head and the tail of the waste liquid channel 112 are respectively connected to the tail of the composite region 103 and the waste liquid pool 113. In this manner, the waste liquid generated in the recombination zone 103 flows into the waste liquid tank 113 through the waste liquid channel 112. Here, in order to prevent backflow of the waste liquid, the waste liquid channel 112 is provided with a backflow-preventing channel gate 114 (not shown in fig. 1), the channel gate 114 being located in particular between the waste liquid reservoir 113 and the rear end of the circulating extrusion region 105.

As shown in fig. 3, the ejection section 4 of the external device associated with the channel gate 114 is described as follows:

the channel gate 114 is disposed on the waste channel 112 and between the waste reservoir 113 and the end of the circulating extrusion zone 105. Fig. 3 is a schematic view of the channel gate 114 in a state of blocking the channel, and referring to fig. 3, the ejector 4 ejects the channel gate 114 downward to block the waste liquid channel 112, thereby preventing the waste liquid in the waste liquid channel 112 and the waste liquid tank 113 from flowing back. When the ejection part 4 is reset, the channel gate 114 is also reset, and the sample or reagent can pass through the waste liquid channel 112 in the reset state. In this embodiment, the channel gate 114 may be a valve plug having a flow channel at a lower end, and the flow channel is connected to the waste liquid channel 112 in a reset state. When the flow channel control is needed, the ejection part 4 presses the valve rubber plug, and the flow channel is pressed until the flow channel is completely closed, so that the waste liquid channel 112 is blocked.

As shown in fig. 2, in the present embodiment, the cyclic extrusion region 105 includes a cyclic extrusion chamber 1051 and a cyclic channel 1052, and the cyclic extrusion chamber 1051 and the recombination region 103 are communicated by the cyclic channel 1052.

In this embodiment, the external instrument applies pressure to compress the cyclic compression chamber 1051, the cyclic compression chamber 1051 applies pressure to the recombination region 103 through the cyclic channel 1052, and the sample or reagent in the recombination region 103 is driven to circularly flow through the recombination region 103 through the cyclic channel 1052, so that the reaction is more complete, and the test result is more accurate. The principle and structure of the driving squeezing area 107 are the same as those of the circulating squeezing area 105, and are not described in detail here.

As shown in FIG. 1, the reagent storage area 104 includes six consecutive storage partitions 1041, and a reagent channel 1042 connecting the storage partition 1041 to the head end of the recombination zone 103. Each compartment includes a reservoir 10411 containing reagent packs and a valve flap (not shown) for opening reagent packs. Wherein, the reagent bag is formed by heat sealing of a reagent envelope, and the reagent is stored in the reagent bag. The valve plate tears the reagent envelope when being stressed by an external instrument, thereby opening the reagent envelope. Each partition is connected to the recombination zone 103 via the reagent channel. In this embodiment, each partition stores a cleaning solution reagent, a luminescent substrate reagent, and an enzyme-labeled antibody reagent. Optionally, the cleaning solution reagent comprises a buffer system, protein and a surfactant; the luminescent substrate reagent comprises a buffer system and an enzymatic chemiluminescent substrate; the enzyme-labeled antibody reagent comprises a buffer system, protein and an enzyme-labeled antibody.

In an application scenario, the buffer system includes one of carbonate, phosphate, Tris (hydroxymethyl) aminomethane (Tris-HCl), and borate; the protein is selected from casein, Bovine Serum Albumin (BSA), etc.; the surfactant comprises one or more of Tween 80, Tween 20, Triton X-100, polyethylene glycol, polyvinylpyrrolidone and glycerol.

Alternatively, the sample inlet channel 101, the sample filtering region 102, the complexing region 103, the reagent storage region 104, the circular squeezing region 105, and the like may be disposed on different surfaces of the chip body.

Optionally, the chip main body comprises a substrate 1, and an upper film 2 and a lower film 3 respectively disposed on the substantially upper and lower surfaces; the substrate 1 is provided with a micro-hole, a micro-channel or a micro-cavity to form the sample inlet channel 101, the sample filtering region 102, the complexing region 103, the reagent storage region 104 and the cyclic extrusion region 105 with the upper membrane 2 or the lower membrane 3.

Fig. 4 shows a specific structure of a chip main body provided by an embodiment of the present invention, as shown in fig. 4, the chip main body includes a substrate 1, an upper membrane 2 and a lower membrane 3, the substrate 1 is provided with micropores, microchannels or microcavity to form the sample inlet channel 101, the sample filtering region 102, the composite region 103, the reagent storage region 104 and the circular extrusion region 105 with the upper membrane 2 or the lower membrane 3, and the sample degassing chamber 111, the driving extrusion region 107, the driving channel 106, the waste liquid tank 113, the waste liquid channel 112, etc. The upper film 2 is provided with micropores to meet the condition that the corresponding part on the substrate is communicated with the atmosphere; the upper film 2 or the lower film 3 has excellent light transmittance so as to detect the intensity of the luminescence signal of the composite region.

Optionally, the substrate, the upper film, and the lower film are made of polyethylene terephthalate (PET), Polybutylene terephthalate (PBT), polypropylene (PP), or Acrylonitrile Butadiene Styrene (ABS).

The utility model provides a flow and the testing result based on above-mentioned chemiluminescence micro-fluidic chip carries out Procalcitonin (PCT) detection in blood as follows:

microfluidic chip assembly

Opening the mould to manufacture a chip substrate, and coating the PCT antibody in the composite area by adopting a high-temperature baking, freeze-drying or low-temperature baking mode and the like; sticking a reagent envelope, and placing a valve plate; the enzyme-labeled antibody reagent is Alkaline phosphatase (ALP) labeled PCT antibody solution, and PH7.6Tris buffer solution containing 1% of bovine serum albumin, 0.2% of Tween 20 and 0.01% of Proclin 300; the wash solution reagent was PH7.4 phosphate buffer containing 0.2% BSA, 0.05% tween 20, and 0.01% Proclin 300; the luminescent substrate reagent is a luminescent substrate solution of basic phosphatase; respectively injecting the enzyme-labeled antibody reagent, the cleaning solution reagent and the luminescent substrate reagent into a reagent storage area and sealing; and (3) sticking a lower membrane, placing a sample filtering membrane, a sample degassing membrane and a channel gate, and finally sticking an upper membrane.

(II) sample detection

Injecting 400 mu L of sample through a sample injection port, covering a gland, filtering the sample by a sample filtering membrane and degassing by a sample degassing membrane, then entering a composite area, and reacting with the PCT antibody coated in the composite area; then, an enzyme-labeled antibody reagent is injected into the composite region through the reagent storage region to react to form a sandwich structure of the ALP-labeled monoclonal antibody-PCT antigen-coated monoclonal antibody card; releasing a cleaning solution reagent to the composite region through the reagent storage region, and cleaning the composite region; then injecting a luminous substrate reagent into the complex region through the reagent storage region to perform reaction. In the process of reaction and cleaning, the sample or the reagent circularly flows for many times in the compound area through the circular extrusion area, so that reactants are fully mixed or contacted, and the reaction is more sufficient. After the reaction is finished, detecting the luminous signal intensity of the chip composite area by an external detection instrument matched with the chemiluminescence micro-fluidic chip, wherein the total detection time is 15 minutes, each sample is respectively measured for 3 times by using 3 chemiluminescence micro-fluidic chips, the average value is taken, a standard curve is drawn, and the concentration of PCT in the sample is obtained according to the standard curve. The higher the PCT content in the sample, the stronger the luminescence signal. The result shows that the correlation coefficient R is more than or equal to 0.99, the repeatability is good, and the reference can be provided for the diagnosis of inflammatory diseases.

In the above embodiments, the descriptions of the respective embodiments have respective emphasis, and reference may be made to the related descriptions of other embodiments for parts that are not described or illustrated in a certain embodiment.

The above-mentioned embodiments are only used for illustrating the technical solution of the present invention, and not for limiting the same; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those skilled in the art; the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the embodiments of the present invention, and should be construed as being included therein.

Claims

1. A chemiluminescent microfluidic chip is characterized by comprising a chip main body, a sample inlet channel, a sample filtering area and a composite area, wherein the sample inlet channel, the sample filtering area and the composite area are arranged on the chip main body and are sequentially communicated, the composite area is used for carrying out immunoreaction and photometry, and an enzyme-labeled antibody is coated in the composite area;

the chemiluminescent microfluidic chip also comprises a reagent storage area which is arranged on the chip main body and used for storing cleaning liquid, a luminescent substrate and an enzyme-labeled antibody, and a circulating extrusion area which is used for driving a sample to circularly flow in the composite area, wherein the head end and the tail end of the circulating extrusion area are respectively communicated with the head end and the tail end of the composite area, and the reagent storage area is communicated with the head end of the composite area.

2. The chemiluminescent microfluidic chip according to claim 1 wherein the sample entry channel is a channel having an inner surface that is physically modified to change its alignment such that the sample can wick at the sample entry channel to flow through the sample filtration zone into the recombination zone.

3. The chemiluminescent microfluidic chip according to claim 1 further comprising a driving channel disposed on the chip body and a driving extrusion region for driving a sample into the recombination region after passing through the sample filtration region; and the head end and the tail end of the driving channel are respectively communicated with the driving extrusion area and the head end of the sample inlet channel.

4. The chemiluminescent microfluidic chip according to claim 1 further comprising a sample injection port disposed at the head end of the sample inlet channel, wherein the sample injection port is covered with a removable cover.

5. The chemiluminescent microfluidic chip according to claim 1 further comprising a sample degassing chamber disposed on the chip body for removing gas in the sample, wherein the ends of the sample degassing chamber are respectively connected to the tail end of the sample filtering region and the head end of the recombination region.

6. The chemiluminescent microfluidic chip according to claim 1 further comprising a waste liquid channel and a waste liquid pool disposed on the chip body, wherein the two ends of the waste liquid channel are respectively connected to the tail end of the recombination region and the waste liquid pool.

7. The chemiluminescent microfluidic chip according to claim 6 further comprising a channel gate for backflow prevention disposed on the chip body, the channel gate being disposed on the waste channel between the waste reservoir and the end of the cyclical extrusion region.

8. The chemiluminescent microfluidic chip according to claim 5 wherein the sample filtration zone has a sample filtration membrane disposed therein and the sample degassing compartment has a sample degassing membrane disposed therein.

9. The chemiluminescent microfluidic chip according to any one of claims 1 to 7 wherein the reagent storage region comprises a storage reservoir containing a reagent pack, a valve flap for opening the reagent pack, and a reagent channel communicating the storage reservoir with the head end of the recombination region.

10. The chemiluminescent microfluidic chip according to any one of claims 1 to 7 wherein the chip body comprises a substrate, and an upper membrane and a lower membrane respectively disposed on the upper and lower surfaces of the substrate; the substrate is provided with micropores, microchannels or microcavity so as to form the sample inlet channel, the sample filtering area, the composite area, the reagent storage area and the circulating extrusion area together with the upper membrane or the lower membrane.