CN210538465U

CN210538465U - Embryo and oocyte cryopreserving device

Info

Publication number: CN210538465U
Application number: CN201921173146.0U
Authority: CN
Inventors: 饶金鹏; 金敏; 金帆; 封纯; 邱枫; 蔡益婷; 田申; 钱小红; 毛愉婵; 金凯红; 朱倩
Original assignee: Zhejiang University ZJU
Current assignee: Zhejiang University ZJU
Priority date: 2019-07-24
Filing date: 2019-07-24
Publication date: 2020-05-19
Anticipated expiration: 2029-07-24

Abstract

The utility model discloses an embryo and oocyte cryopreserving device relates to supplementary reproduction technical field, including carrier and cryopreserving box, the carrier includes supporting part, clamping part and protective cap cover, and the rear end of supporting part is connected with the front end detachably of clamping part, and the front end of supporting part is connected with the rear end detachably of protective cap cover. The bearing part comprises a base, a slide glass is arranged at the front end of the base, the clamping part comprises an outer tube and a push rod, and a plurality of elastic sheets are arranged on the outer tube at intervals; the front end of the clamping part is inserted into the cavity through the opening, and the front end of the elastic sheet is inserted into the cavity and abutted against the inner side wall of the cavity; the outer tube and the base are communicated to form a channel for the push rod to slide, the protective cap sleeve is detachably connected with the connecting piece, the cryopreservation box comprises a box body and a cover body, and a plurality of storage groove chambers are arranged on the box body. The utility model provides an among the prior art because of embryo or oocyte extremely freeze the freezing pole that adopts of carrying and occupy more storage space's technical problem, very big saving storage space.

Description

Embryo and oocyte cryopreserving device

Technical Field

The application relates to the technical field of assisted reproduction, in particular to an embryo and oocyte cryopreservation device.

Background

With the release of the comprehensive policy of China 'two children', the number of couples meeting the standards of two children in China is about 9000 ten thousand pairs, wherein 6000 million women are over 35 years old, and along with the superimposed influence of factors such as environmental pollution, high working and living pressure, age increase, bad life style and the like, according to the research report of the current situation of infertility in China issued by China Association of population and the United nations of China Committee of family planning, the incidence rate of infertility in China is 12.5% -15%, and the number of patients is about 4000 million. To achieve the goal of fertility, the most effective way is by Assisted Reproductive Technology (ART) represented by in vitro fertilization-embryo transfer (IVF-ET).

In the case of IVF-ET in the embryo laboratory, the female oocyte is taken out on the day of the ovum-taking operation, but the male cannot take out the sperm for various reasons, and the female is in urgent need of fertility preservation before receiving chemotherapy or radiotherapy due to malignant tumor, and at this time, the female oocyte needs to be frozen. When the embryo is developed in vitro to a certain stage, the number of available embryos is larger than the number of transferred embryos or the endometrium of the female is not in the optimal embryo transfer stage, and the like, the embryos need to be subjected to cryopreservation operation. Currently, freezing of oocytes or embryos is largely divided into slow freezing and extreme freezing. Compared with slow programmed freezing, the ultra-fast freezing method does not need to purchase expensive programmed freezers, and has the advantages of simple operation, time saving, good freezing effect and the like, so that the method is adopted by most embryo laboratories.

At present, long rod-shaped freezing carrying rods are generally adopted as carriers in the extremely-fast cryopreservation of embryos or oocytes by the reproductive medicine centers at home and abroad. However, due to the restriction of the external shape, the carrier is adopted, about 2000 embryos can be stored in a widely used American MVE liquid nitrogen container with the capacity of 47.4 liters (2 embryos are loaded on 1 carrying rod, 5 carrying rods are contained in 1 aluminum strip, 20 aluminum strips are loaded on 1 bucket, 10 buckets are arranged in 1 liquid nitrogen container, 2, 5, 20, 10 and 2000 are filled in the 1 aluminum strip, if more carrying rods are forcibly plugged, the carrying rods and the carrying rods are squeezed with each other, so that the amount of liquid nitrogen at the periphery of the carrying rods is insufficient, the situation that the carrying rods are exposed out of the liquid nitrogen in the lifting process is easy to occur, and the survival and recovery of the embryos or the oocytes are greatly influenced.

Therefore, the prior art has at least the following technical problems: the freezing carrying rod used for the extremely-fast freezing storage of the embryo or the oocyte occupies more storage space.

SUMMERY OF THE UTILITY MODEL

The embodiment of this application is through providing an embryo and oocyte cryopreserving device for solve among the prior art because of embryo or oocyte extremely fast cryopreserving adopts freezing the technical problem that the pole occupies more storage space. The embodiment of this application is through bearing part, clamping part and the protective cap cover that becomes the carrier split and can freely connect and separate, can deposit the front in embryo or the utmost point quick-freeze of oocyte, leave away out the great clamping part of volume, only need deposit in the liquid nitrogen container again after putting into the cryopreservation box with the less bearing part of volume and protective cap cover, compare the very big storage space that has saved with freezing year pole among the prior art, effectively improved the utilization ratio of liquid nitrogen container to storage cost has been reduced.

In order to solve the above problems, an embodiment of the present application provides an embryo and oocyte cryopreservation device, the cryopreservation device includes a carrier and a cryopreservation box, the carrier includes a bearing portion, a clamping portion and a protective cap, a rear end of the bearing portion is detachably connected with a front end of the clamping portion, and a front end of the bearing portion is detachably connected with a rear end of the protective cap;

the bearing part comprises a base, the front end of the base is connected with a slide glass for bearing an embryo or an oocyte through a connecting piece, a cavity is arranged in the base, an opening is arranged at the rear end of the base, and the opening is communicated with the cavity;

the clamping part comprises an outer tube and a push rod which is arranged in the outer tube in a sliding mode, and the push rod coaxially penetrates through the outer tube; a plurality of elastic sheets are arranged on the outer pipe at intervals, the rear ends of the elastic sheets are fixed on the outer wall of the outer pipe, and the front ends of the elastic sheets extend rightwards along the axial direction and are opened towards the outside of the outer pipe;

the bearing part and the clamping part are detachably connected through the base and the elastic sheet, the front end of the clamping part is inserted into the cavity through the opening, and the front end of the elastic sheet is inserted into the cavity and abuts against the inner side wall of the cavity; the outer tube is communicated with the base to form a channel for the push rod to slide so as to allow the push rod to push the base;

the rear end of the protective cap sleeve is provided with a sleeve opening which is used for covering the slide glass, the protective cap sleeve is detachably connected with the connecting piece, and the front end of the protective cap sleeve is sealed;

the freezing storage box comprises a box body and a cover body, and a plurality of storage groove chambers are arranged on the box body.

Furthermore, the connecting piece is cylindrical, an external thread is arranged on the outer wall of the connecting piece, an internal thread is arranged on the inner wall of the protective cap sleeve, and the connecting piece and the protective cap sleeve are detachably connected through the threaded connection matching of the internal thread and the external thread.

Further, the base is in a hollow regular prism shape, the cross section of the base is provided with n edges, and the number of the elastic sheets is

And n is a positive integer.

Furthermore, a label sticking or information marking area is arranged on the outer wall of the base.

Furthermore, the elastic sheets are arranged on the outer pipe at equal intervals, and the projection centers of the elastic sheets on the cross section of the base are symmetrical.

Further, the storage groove chambers are distributed on the box body in a matrix mode.

Furthermore, a balancing weight is arranged on the inner end face of the front end of the protective cap sleeve.

Furthermore, one side of the slide glass is provided with a first mark.

Further, the cover body is transparent.

Furthermore, a second mark is arranged on the cover body corresponding to each storage groove chamber.

One or more technical solutions in the embodiments of the present application have at least one or more of the following technical effects:

1. bearing part and clamping part detachably in the embodiment of this application are connected, can freely connect and separate, this just makes when freezing embryo or oocyte, leave away out the great grip part of volume, only need to put into freezing box with the less bearing part of volume and protective cap cover and deposit in the liquid nitrogen container again, compare very big saving storage space with the freezing pole that generally uses at present, the biggest memory space of single liquid nitrogen container is risen to 6000 embryos by 2000 embryos, this not only has improved the space utilization of embryo freezer, the freezing pole that has effectively solved among the prior art occupies more storage space's technical problem, and avoided purchasing too much import storage liquid nitrogen container, its cost also obtains control when liquid nitrogen regularly adds the maintenance at ordinary times, has better practical value.

2. In the embodiment of the application, the front end of the elastic sheet is inserted into the cavity and abutted against the inner side wall of the cavity, the elastic sheet along the radial direction of the outer tube can play a role in effectively connecting the bearing part with the clamping part, and the bearing part and the protective cap sleeve can be screwed when the clamping part is effectively held.

3. In the embodiment of the application through-hole in the outer tube with the inner chamber intercommunication of base, the push rod can the through-hole with slide in the base, when needs the bearing part with during the clamping part separation, will the push rod promotes extremely in the base, promote the push rod, the push rod can with the base is followed the clamping part pushes up down, thereby makes the bearing part with the clamping part separates, and the operation is very convenient and fast.

4. The connecting piece in the embodiment of the application is cylindrical, so that the bearing part and the protective cap sleeve can be reliably screwed, and the bearing part and the protective cap sleeve are prevented from being separated due to loose connection.

5. The base in the embodiment of the application is in a hollow regular prism shape, the cross section of the base is provided with n edges, and the number of the elastic sheets is

And n is a positive integer, so that the elastic sheet can be tightly propped against the inner wall of the cavity, and the handheld part is effectively connected with the bearing part.

6. The label sticking or information marking area in the embodiment of the application is used for marking relevant information of a patient, for example, information such as the name, the case number and the like of the patient is marked on the label sticking or information marking area by using a special liquid nitrogen-proof marker pen or printed information label paper. The base is provided with the label sticking or information marking area, so that each bearing part can effectively mark the information of the embryo or the oocyte, and the identity confusion before and after the embryo or the oocyte is frozen and thawed is prevented.

7. In the embodiment of the application, the elastic sheets surround the outer tube in a circle along the circumferential direction of the outer tube, and the projection centers of the elastic sheets on the cross section of the base are symmetrical, so that the outer tube and the base 1 can be stably connected.

8. In the embodiment of the application, the inner end face of the front end of the protective cap sleeve is provided with the balancing weight, and the balancing weight can be a metal block with high density, such as an iron block and the like, so that the slide glass or the protective cap sleeve is prevented from floating out of the liquid nitrogen surface due to too light mass, and embryos or oocytes are prevented from dying.

9. The slide glass in the embodiment of the application is a sterile polypropylene sheet, the slide glass is divided into a front surface and a back surface, the front surface and the front end are coated with a first mark, the first mark can be an indicating color (such as red), and the back surface is colorless. The indicating color coated on the front end of the front surface of the slide enables an operator to identify which surface of the slide the embryo or the oocyte is positioned on by naked eyes, so that the damage of downward physical extrusion to the embryo caused by the fact that the embryo is positioned below the slide in the unfreezing process can be avoided.

10. In this application embodiment the lid is transparent and be compiled with the sequence number of holding vessel room one-to-one then makes can discern embryo and oocyte position fast when depositing and take out, avoids looking for the time overlength and produces the adverse effect to embryo or oocyte, and the setting up of serial number makes the management of freezing embryo or oocyte more orderly and high-efficient in addition.

11. In the embodiment of the application, one of the storage tank chambers on the freezing storage box is provided with the third mark, so that a user can be helped to distinguish the starting and stopping positions of the storage tank chambers.

The foregoing description is only an overview of the technical solutions of the present application, and the present application can be implemented according to the content of the description in order to make the technical means of the present application more clearly understood, and the following detailed description of the present application is given in order to make the above and other objects, features, and advantages of the present application more clearly understandable.

Drawings

Fig. 1 is a schematic structural diagram of a carrier of an embryo and oocyte cryopreservation device according to an embodiment of the present invention;

fig. 2 is a schematic view of a projection structure of a clamping portion of an embryo and oocyte cryopreservation device on a base according to an embodiment of the present invention;

FIG. 3 is a schematic diagram of a projection structure of a holding portion of an embryo and oocyte cryopreservation device on a base according to another embodiment of the present invention;

FIG. 4 is a schematic structural diagram of a case of an embryo and oocyte cryopreservation device according to an embodiment of the present invention;

fig. 5 is a schematic structural diagram of a cover of an embryo and oocyte cryopreservation device according to an embodiment of the present invention.

Description of reference numerals: the bearing part 1, the slide glass 11, the indication color, the reverse side 112, the connecting piece 12, the external thread 121, the base 13, the label pasting or information marking area 131, the clamping part 2, the elastic sheet 21, the push rod 22, the through hole 23, the protective cap sleeve 3, the internal thread 31, the balancing weight 32, the cryopreservation box 4, the box body 41, the storage tank chamber 411, the third mark 4111 and the cover body 42.

Detailed Description

The embodiment of the application provides an embryo and oocyte cryopreserving device for solve the technical problem that the freezing carrying rod adopted by the embryo or oocyte cryopreservation in the prior art occupies more storage space.

In order to solve the technical problems, the technical scheme provided by the application has the following general idea: through bearing part, clamping part and the protective cap cover that with the carrier split for freely connecting and separating, can leave away out the great clamping part of volume before embryo or oocyte utmost point quick-freeze is deposited, only need put into the less bearing part of volume and protective cap cover and deposit in the liquid nitrogen container after freezing the box, compare very big storage space of having saved with freezing carrier bar among the prior art, effectively improved the utilization ratio of liquid nitrogen container to storage cost has been reduced.

The technical solutions of the present application are described in detail below with reference to the drawings and specific embodiments, and it should be understood that the specific features in the embodiments and examples of the present application are detailed descriptions of the technical solutions of the present application, and are not limitations of the technical solutions of the present application, and the technical features in the embodiments and examples of the present application may be combined with each other without conflict.

The utility model provides an embryo and oocyte cryopreserving device, cryopreserving device includes carrier and cryopreserving box 4, as shown in figure 1, the carrier includes supporting part 1, clamping part 2 and protective cap cover 3, just the rear end of supporting part 1 with the front end detachably of clamping part 2 connects, the front end of supporting part 1 with the rear end detachably of protective cap cover 3 connects.

Specifically, the left end shown in fig. 1 is used as the rear end, the right end shown in fig. 1 is used as the front end, and the rear end and the front end are only for convenience of description and are not limitations to the technical solution of the present invention.

Specifically, the bearing part 1 and the protective cap 3 are small, and the clamping part 2 is convenient to hold by hands and large in size. Bearing part 1 with 2 detachably of clamping part connect, can deposit preceding at embryo or the utmost point quick-freeze of oocyte, separate away 2 clamping parts that the volume is great, only need to put into the less bearing part 1 of volume and protective cap cover 3 and deposit in the liquid nitrogen container again after depositing box 4 with freezing and store, carry the pole with freezing among the prior art and compare very big save storage space, effectively improved the utilization ratio of liquid nitrogen container to storage cost has been reduced.

The bearing part 1 comprises a base 13, the front end of the base 13 is connected with a slide 11 for bearing an embryo or an oocyte through a connecting piece 12, a cavity is arranged inside the base 13, and the rear end of the base 13 is provided with an opening communicated with the cavity.

The clamping part 2 comprises an outer tube and a push rod 22 which is slidably arranged in the outer tube, and the push rod 22 coaxially penetrates through the outer tube; the outer tube is provided with a plurality of elastic sheets 21 at intervals, the rear ends of the elastic sheets 21 are fixed on the outer wall of the outer tube, and the front ends of the elastic sheets 21 extend rightwards along the axial direction and are opened out of the outer tube.

Specifically, the axial direction refers to a central axis direction of the outer tube or a direction parallel to the central axis. A through hole 23 for the push rod 22 to slide is axially arranged in the outer tube, and the push rod 22 can slidably penetrate through the through hole 23.

The bearing part 1 and the clamping part 2 are detachably connected through the base 13 and the elastic sheet 21, the front end of the clamping part 2 is inserted into the cavity through the opening, and the front end of the elastic sheet 21 is inserted into the cavity and abuts against the inner side wall of the cavity; the outer tube is communicated with the base 13 to form a channel for the push rod 22 to slide, so as to allow the push rod 22 to push the base.

Specifically, the front end of the elastic sheet 21 is inserted into the cavity and abuts against the inner side wall of the cavity, and the elastic sheet 21 can play a role in effectively connecting the bearing part 1 with the clamping part 2 along the radial elasticity of the outer tube, so that the bearing part 1 and the protective cap sleeve 3 can be screwed when the clamping part 2 is held.

Particularly, the through-hole in the outer tube with the inner chamber intercommunication of base 13, push rod 22 can the through-hole with the passageway that base 13 constitutes slides, when needs the bearing part 1 with when the clamping part 2 separates, will push rod 22 promote to in the base 23, promote push rod 22, push rod 22 can with base 13 follow clamping part 2 pushes down, thereby makes bearing part 1 with the separation of clamping part 2, and the operation is very convenient and fast.

The rear end of the protective cap sleeve 3 is provided with a sleeve opening used for covering the slide glass 11, the protective cap sleeve 3 is detachably connected with the connecting piece 12, and the front end of the protective cap sleeve 3 is sealed.

The cryopreservation box 4 comprises a box body 41 and a cover body 42, wherein the box body 41 is provided with a plurality of storage tank chambers 411.

Specifically, the reservoir chamber 411 is a recess with an open top.

Bearing part 1 in this embodiment is connected with 2 detachably of clamping part, can freely connect and separate, this just makes when freezing embryo or oocyte, leave away out the great grip part of volume, only need to put into freezing box with the less bearing part 1 of volume and protective cap cover 3 and then deposit in the liquid nitrogen container, compare very big saving storage space with the freezing pole of present generally used, the biggest memory space of single liquid nitrogen container rises to 6000 embryos by 2000 pieces of embryos, this not only has improved the space usage rate of embryo freezer, effectively solved the freezing pole among the prior art and taken up the technical problem in more storage space, and avoided purchasing too much import storage liquid nitrogen container, its cost also obtains control when liquid nitrogen regularly adds the maintenance at ordinary times, has better practical value.

Further, the connecting piece 12 is cylindrical, an external thread 121 is arranged on the outer wall of the connecting piece 12, an internal thread 31 is arranged on the inner wall of the protective cap sleeve 3, and the connecting piece 12 and the protective cap sleeve 3 are detachably connected through the threaded connection of the internal thread 31 and the external thread 121.

Specifically, the connecting member 12 is cylindrical, so that the bearing part 1 and the protective cap 3 can be reliably screwed, and the bearing part 1 and the protective cap 3 are prevented from being separated due to loose insertion.

Further, the base 13 is in the shape of a hollow regular prism, the cross section of the base 13 is provided with n sides, and the number of the elastic sheets 21 is

And n is a positive integer.

Specifically, as shown in fig. 2, when the base 13 is a regular hexagonal prism, the cross-sectional shape of the base has 6 sides, and the number of the resilient pieces 21 is 3; as shown in fig. 3, when the base 13 is a regular octagonal prism, the cross-sectional shape of the base 13 has 8 sides, and the number of the elastic pieces 21 is 4, so that the elastic pieces can uniformly abut against the inner wall of the cavity along the circumferential direction, and the handheld portion 2 and the bearing portion 1 can be effectively connected.

Further, a label sticking or information marking area 131 is disposed on the outer wall of the base 13.

Specifically, the label sticking or information marking area 131 is used for marking information related to the patient, and information such as the name and the patient's case number of the patient is marked on the label sticking or information marking area 131 with a liquid nitrogen-proof special marker or a printed information label paper. The base is provided with a label sticking or information marking area 131, so that each bearing part can effectively mark the information of the embryo or the oocyte, and the identity confusion before and after the embryo or the oocyte is frozen and thawed can be prevented.

Furthermore, the elastic pieces 21 are arranged on the outer tube at equal intervals, and the projection centers of the elastic pieces 21 on the cross section of the base 13 are symmetrical.

Specifically, the elastic sheets 21 enclose a circle on the outer tube along the circumferential direction of the outer tube, and the projection centers of the elastic sheets 21 on the cross section of the base 13 are symmetrical, so that the outer tube and the base 13 can be stably connected.

Further, the storage slot chambers 411 are distributed on the box body 41 in a matrix.

Further, a balancing weight 32 is arranged on the inner end face of the front end of the protective cap sleeve 3.

Specifically, the weight 32 is made of stainless steel to prevent the slide 11 or the protective cap 3 from floating out of the liquid nitrogen surface due to too light weight, thereby preventing the embryo or the oocyte from dying.

Further, a plug convenient for holding is arranged at the rear end of the push rod 22.

Further, a first mark 111 is provided on one surface of the slide 11.

Specifically, the slide 11 is a sterile polypropylene sheet, the slide 11 has front and back surfaces, the front surface of the front surface is coated with a first mark 111, the first mark 111 may be an indicator color (e.g., red), and the back surface 112 is colorless. The front end of the front surface of the slide 11 is coated with the indicating color, so that an operator can identify which surface of the slide the embryo or the oocyte is positioned on by naked eyes, and the damage to the downward physical extrusion of the embryo caused by the fact that the embryo is positioned below the slide in the unfreezing process can be avoided.

Further, the cover 42 is transparent, and a second mark is disposed on the cover 42 corresponding to each storage slot compartment 411.

Specifically, the second mark is a serial number, the cover body 42 is transparent and is coded with the serial number corresponding to the storage groove chamber 41 one by one, so that the positions of the embryo and the oocyte can be quickly identified when the embryo and the oocyte are stored and taken out, the adverse effect on the embryo or the oocyte caused by overlong search time is avoided, and the management of freezing the embryo or the oocyte is more orderly and efficient due to the arrangement of the serial number.

Further, one of the storage tank compartments 411 of the cryopreservation box is provided with a third mark 4111.

Specifically, for example, a third mark 4111 (which may be a color mark such as yellow) is disposed on the first reservoir chamber 411 at the upper left corner to help the user distinguish the start and stop positions of the reservoir chamber 411.

The application method of the embryo and oocyte cryopreservation device comprises the following steps:

1) firstly, information such as the name, the case number and the like of a patient is marked on the label sticking or information marking area 131 on the base 13 by using a special liquid nitrogen-proof marker pen or printed information label paper;

2) extending the elastic sheet 21 into the base 13 of the bearing part 1, enabling the clamping part 2 to be connected with the bearing part 1 by the outward elastic force of the elastic sheet 21, placing the connected clamping part 2 and the bearing part 1 at the near side of a microscope of an ultra-clean workstation, and waiting for the slide 11 on an embryo or an oocyte;

3) pouring liquid nitrogen into the cryopreservation box 4, enabling the liquid level of the liquid nitrogen to exceed the upper surface of a box body 41 of the cryopreservation box 4, inserting the sleeve opening of the protective cap sleeve 3 upwards into a storage tank chamber 411 at a specific position of the box body 41 of the cryopreservation box 4, enabling the inside and the outside of the protective cap sleeve 3 to be filled with the liquid nitrogen, and ensuring that the liquid level of the liquid nitrogen is higher than the upper surface of the protective cap sleeve 3; at the moment, the embryo or the oocyte is placed in permeable cryoprotectant and impermeable cryoprotectant with gradually increasing concentration to complete the replacement of the intracellular fluid and the external cryoprotectant;

4) after the last step of displacement is finished and the embryo or the oocyte is sucked into the capillary glass tube, the rear end of the clamping part 2 is held by hands, the bearing part 1 is moved to the position of a microscope light source, the focal length of the microscope is adjusted, the embryo or the oocyte is loaded to one side of the front surface of the sterile polypropylene slide 11 coated with the indication color, and the redundant cryoprotectant around the embryo or the oocyte is sucked;

5) rapidly holding the rear end of the clamping part 2 by hand, inserting the slide 11 into the protective cap sleeve 3 which is placed in liquid nitrogen, and tightly screwing the micro bearing part 1 bearing the embryo or the oocyte with the protective cap sleeve 3 through the thread 121 of the bearing part connecting piece 12 and the thread 31 at the front end of the protective cap sleeve 3;

6) the long rod 22 is pushed forwards, the clamping part 2 is separated from the bearing part 1 under the action of the pushing force, the transparent cover body 42 is covered, and the freezing box 2 is put back into the liquid nitrogen tank, namely the whole quick freezing storage process is completed.

In summary, the embryo and oocyte cryopreservation device described in this embodiment has at least the following technical effects:

1. bearing part 1 in the embodiment of the application is connected with 2 detachably of clamping part, can freely connect and separate, this just makes when freezing embryo or oocyte, leave away out the great grip part of volume, only need to put into freezing box with the less bearing part 1 of volume and protective cap cover 3 and then deposit in the liquid nitrogen container, compare very big saving storage space with the freezing pole that uses generally at present, the biggest memory space of single liquid nitrogen container rises to 6000 embryos by 2000 pieces of embryos, this not only has improved the space usage of embryo freezer, the freezing pole that has effectively solved among the prior art takes up the technical problem of more storage space, and avoided purchasing too much import storage liquid nitrogen container, its cost also obtains control when liquid nitrogen regularly adds the maintenance at ordinary times, has better practical value.

2. In the embodiment of the present application, the front end of the elastic sheet 21 is inserted into the cavity and abutted against the inner side wall of the cavity, the elastic sheet 21 along the radial direction of the outer tube can play a role in effectively connecting the bearing part 1 with the clamping part 2, and the clamping part 2 can be effectively held by a screw joint between the bearing part 1 and the protective cap sleeve 3.

3. In the embodiment of this application the through-hole in the outer tube with the inner chamber intercommunication of base 13, push rod 22 can the through-hole with slide in the base 13, when needs bearing part 1 with during the separation of clamping part 2, will push rod 22 promotes to in the base 23, promote push rod 22, push rod 22 can with base 13 is followed clamping part 2 pushes up down, thereby makes bearing part 1 with the separation of clamping part 2, and the operation is very convenient and fast.

4. The connecting piece 12 in the embodiment of the application is cylindrical, so that the bearing part 1 and the protective cap sleeve 3 can be reliably screwed, and the bearing part 1 and the protective cap sleeve 3 are prevented from being separated due to loose connection.

5. In the embodiment of the present application, the base 13 is in the shape of a hollow regular prism, the cross-sectional shape of the base 13 has n sides, and the number of the elastic pieces 21 is

And n is a positive integer, so that the elastic sheet can be tightly propped against the inner wall of the cavity, and the handheld part 2 and the bearing part 1 are effectively connected.

6. The label sticking or information marking area 131 in the embodiment of the present application is used for marking the relevant information of the patient, for example, the information such as the name, the patient's case number, etc. of the patient is marked on the label sticking or information marking area 131 by using a special liquid nitrogen-proof marker or a printed information label paper. The base is provided with the label sticking or information marking area, so that each bearing part can effectively mark the information of the embryo or the oocyte, and the identity confusion before and after the embryo or the oocyte is frozen and thawed is prevented.

7. In the embodiment of the present application, the elastic sheets 21 form a circle on the outer tube along the circumferential direction of the outer tube, and the projection centers of the elastic sheets 21 on the cross section of the base 13 are symmetrical, so as to ensure that the outer tube and the base 13 can be stably connected.

8. In the embodiment of the present application, the inner end surface of the front end of the protective cap sleeve 3 is provided with the balancing weight 32, and the balancing weight 32 may be a metal block with a large density, such as an iron block, so as to prevent the slide 11 or the protective cap sleeve 3 from floating out of the liquid nitrogen surface due to an excessively light mass, thereby causing death of an embryo or an oocyte.

9. The slide 11 in the embodiment of the present application is a sterile polypropylene sheet, the slide 11 has front and back surfaces, the front surface of the front surface is coated with a first mark 111, the first mark 111 may be an indicating color (e.g., red), and the back surface 112 is colorless. The front end of the front surface of the slide 11 is coated with the indicating color, so that an operator can identify which surface of the slide the embryo or the oocyte is positioned on by naked eyes, and the damage to the downward physical extrusion of the embryo caused by the fact that the embryo is positioned below the slide in the unfreezing process can be avoided.

10. In this application embodiment the lid 42 is transparent and be compiled with the sequence number of holding tank room 41 one-to-one, then make when depositing and take out can discern embryo and oocyte position fast, avoid the seek time overlength and produce the adverse effect to embryo or oocyte, the setting up of serial number makes the management to embryo or oocyte cryopreserving more orderly and high-efficient in addition.

11. In the embodiment of the present application, one of the storage tank chambers 411 on the cryopreservation box is provided with a third mark, which can help a user to distinguish the start and stop positions of the storage tank chambers.

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the examples, those skilled in the art should understand that the technical solutions of the present invention can be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the scope of the claims of the present invention.

Claims

1. The utility model provides an embryo and oocyte cryopreserving device which characterized in that: the cryopreservation device comprises a carrier and a cryopreservation box, wherein the carrier comprises a bearing part, a clamping part and a protective cap sleeve, the rear end of the bearing part is detachably connected with the front end of the clamping part, and the front end of the bearing part is detachably connected with the rear end of the protective cap sleeve;

the clamping part comprises an outer tube and a push rod which is arranged in the outer tube in a sliding mode, and the push rod coaxially penetrates through the outer tube; the outer pipe is provided with a plurality of elastic sheets at intervals, the rear ends of the elastic sheets are fixed on the outer wall of the outer pipe, and the front ends of the elastic sheets extend rightwards along the axial direction and are opened towards the outside of the outer pipe;

2. The embryo and oocyte cryopreservation device of claim 1 wherein: the connecting piece is cylindrical, an external thread is arranged on the outer wall of the connecting piece, an internal thread is arranged on the inner wall of the protective cap sleeve, and the connecting piece and the protective cap sleeve are detachably connected through the threaded connection matching of the internal thread and the external thread.

3. The embryo and oocyte cryopreservation device of claim 1 wherein: the base is in the shape of a hollow regular prism, the cross section of the base is provided with n edges, and the number of the elastic sheets is

And n is a positive integer.

4. The embryo and oocyte cryopreservation device of claim 1 wherein: and a label sticking or information marking area is arranged on the outer wall of the base.

5. The embryo and oocyte cryopreservation device of claim 1 wherein: the elastic sheets are arranged on the outer pipe at equal intervals, and the projection centers of the elastic sheets on the cross section of the base are symmetrical.

6. The embryo and oocyte cryopreservation device of claim 1 wherein: the storage tank chambers are distributed on the box body in a matrix manner.

7. The embryo and oocyte cryopreservation device of claim 1 wherein: and a balancing weight is arranged on the inner end surface of the front end of the protective cap sleeve.

8. The embryo and oocyte cryopreservation device of claim 1 wherein: one side of the slide is provided with a first mark.

9. The embryo and oocyte cryopreservation device of claim 1 wherein: the cover body is transparent.

10. The embryo and oocyte cryopreservation device of claim 1 wherein: and a second mark is arranged on the cover body corresponding to each storage groove chamber.