CN210526908U - Frozen stock solution adding device in umbilical cord blood hematopoietic stem cell separation process - Google Patents
Frozen stock solution adding device in umbilical cord blood hematopoietic stem cell separation process Download PDFInfo
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- CN210526908U CN210526908U CN201921211802.1U CN201921211802U CN210526908U CN 210526908 U CN210526908 U CN 210526908U CN 201921211802 U CN201921211802 U CN 201921211802U CN 210526908 U CN210526908 U CN 210526908U
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Abstract
The utility model discloses a frozen stock solution adding device in a cord blood hematopoietic stem cell separation process, which comprises a base, a fixed frame fixedly connected with the base, an ice bath barrel, a blood bag shaking mechanism, an injector bracket, a push plate, a push rod and a press plate; the ice bath barrel is placed on the base, the blood bag shaking mechanism is arranged above the ice bath barrel, and two ends of a clamping frame of the blood bag shaking mechanism are fixedly connected with the fixing frame; an injector bracket is arranged above the blood bag shaking mechanism, and two ends of the injector bracket are fixedly connected with the fixed frame. The advantages are that: the utility model discloses can add the frozen stock liquid to a plurality of blood bags simultaneously, the accessible is manual presses the clamp plate for the push pedal promotes the syringe simultaneously and realizes synchronous injection, can also promote the push pedal through electric cylinder, can guarantee that the push pedal bulldozes the syringe at the uniform velocity, guarantees to add the frozen stock liquid at the uniform velocity, guarantees to add the process and satisfies the experimental requirement, ensures that cord blood hematopoietic stem cell is reliably effectively preserved.
Description
The technical field is as follows:
the utility model relates to a navel cord blood hematopoietic stem cell separation field especially relates to a frozen stock solution in navel cord blood hematopoietic stem cell separation technology adds device.
Background art:
based on the special biological characteristics of the stem cells, the stem cells have wide application prospects in the treatment of tissue and organ injuries, degenerative diseases and various intractable diseases, and the hematopoietic stem cells are derived from the following sources: bone marrow, peripheral blood and neonatal umbilical cord blood, wherein umbilical cord blood hematopoietic stem cells have the advantages of rich sources, no harm to mother and infant in collection, low matching requirement, instant taking and use and the like, and are the best way for obtaining hematopoietic stem cells.
In order to extract the cord blood hematopoietic stem cells, the cord blood needs to be subjected to a separation experiment, the stem cells finally extracted after separation need to be stored in liquid nitrogen at the temperature of-196 ℃ at a cryogenic temperature, and in order to protect the cells from being damaged in a low-temperature environment, a frozen stock solution needs to be added to the cord blood hematopoietic stem cells to serve as a cord blood hematopoietic stem cell protective agent. And the process of adding the frozen stock solution requires slow and uniform adding, and the blood bag is shaken in the adding process, so that the frozen stock solution and the umbilical cord blood hematopoietic stem cells are fully and uniformly mixed. At present, in the process of adding frozen stock solution, a blood bag is usually connected with an injector and is manually added by a worker through the injector, but the adding speed cannot be well controlled, so that the operation difficulty is high; meanwhile, the frozen stock solution is often required to be added to a plurality of blood bags, a plurality of syringes cannot be operated simultaneously by one hand, the plurality of blood bags are required to be added one by one, the adding efficiency is low, and the experiment period and the experiment cost are increased; and in the process of adding frozen stock solution one by one to a plurality of blood bags, the blood bags need to be repeatedly shaken, time and labor are wasted, the arms of workers are tired, the shaking frequency and the shaking speed can be reduced along with the addition work, the combination speed of the protective agent and the cells is uneven, the mixing is insufficient, the protective agent cannot be fully used for protection when the protective agent is stored at low temperature after separation, and the damage to the stem cells is caused.
The utility model has the following contents:
an object of the utility model is to provide a frozen stock solution adds device in labour saving and time saving, reliable effectual cord blood hematopoietic stem cell separation technology.
The utility model discloses by following technical scheme implement:
the frozen stock solution adding device in the cord blood hematopoietic stem cell separation process comprises a base, a fixing frame fixedly connected with the base, an ice bath barrel, a blood bag shaking mechanism, an injector support, a push plate, a push rod and a press plate;
the ice bath barrel is placed on the base, the blood bag shaking mechanism is arranged above the ice bath barrel, the top end of a rocker of the blood bag shaking mechanism is rotatably connected with the fixing frame, and a shell of a motor of the blood bag shaking mechanism is fixedly connected with the fixing frame; the injector support is arranged above the blood bag shaking mechanism, two ends of the injector support are fixedly connected with the fixing frame, and a plurality of placing holes matched with the injector in size are formed in the injector support; the push plate is arranged above the injector support, sliding blocks are fixedly arranged at two ends of the push plate, sliding grooves matched with the sliding blocks are formed in the opposite inner sides of the fixing frame above the injector support, and the sliding blocks are arranged in the sliding grooves in a sliding mode; a through hole is formed in the fixing frame right above the push plate, the bottom end of the push rod is fixedly connected with the push plate, and the top end of the push rod penetrates through the through hole and is fixedly connected with the pressing plate.
Further, the blood bag shaking mechanism comprises the rocker, a clamping frame, a clamping plate, a limiting clamp, the motor, a speed reducer and a cam;
the bottom end of the rocker is fixedly connected with the clamping frame, an output shaft of the motor is in transmission connection with an input end of the speed reducer, the output shaft of the speed reducer is fixedly provided with the cam, and the edge of the cam is abutted to the rocker;
the clamping frame is fixedly provided with clamping grooves in the vertical direction, the number of the clamping grooves is equal to that of the placing holes, the clamping plate is arranged on one side, provided with the clamping grooves, of the clamping frame, one end of the clamping plate is rotatably connected with the clamping frame, a limiting card is integrally arranged on the clamping frame, the position of the limiting card is opposite to that of the other end of the clamping plate, and the other end of the clamping plate is movably clamped in the limiting card.
Furthermore, the electric heating device further comprises an electric cylinder which is vertically arranged, the cylinder body of the electric cylinder is fixedly connected with the fixing frame, and the piston rod of the electric cylinder is fixedly connected with the push plate.
The utility model has the advantages that:
the utility model discloses can add frozen stock solution to a plurality of blood bags simultaneously, the clamp plate is pressed to the accessible manual pressure for the push pedal promotes the syringe simultaneously and realizes synchronous injection, can also promote the push pedal through electric cylinder, can guarantee the push pedal bulldozes the syringe at the uniform velocity, guarantees to add frozen stock solution at the uniform velocity, guarantees that the interpolation process satisfies the experimental requirement, guarantees that umbilical cord blood hematopoietic stem cell is reliably effectively preserved, can also effectively improve work efficiency; in addition, manual shaking is not needed, the workload is reduced, the shaking frequency is consistent, the shaking effect is good, the protective agent can fully play a role, and stem cells are prevented from being damaged.
Description of the drawings:
in order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a state diagram of the present embodiment;
FIG. 2 is an enlarged view of a portion of FIG. 1 at A;
in the figure: base 1, mount 2, ice bath bucket 3, blood bag rock mechanism 4, holding frame 4.1, draw-in groove 4.2, rocker 4.3, cardboard 4.4, spacing card 4.5, motor 4.6, speed reducer 4.7, cam 4.8, syringe support 5, place hole 6, push pedal 7, spout 8, slider 9, push rod 10, clamp plate 11, through-hole 12, electric cylinder 13, syringe 14, blood bag 15, blood transfusion tube 16.
The specific implementation mode is as follows:
the technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example 1:
the frozen stock solution adding device in the cord blood hematopoietic stem cell separation process shown in fig. 1 and 2 comprises a base 1, a fixing frame 2 fixedly connected with the base 1, an ice bath barrel 3, a blood bag shaking mechanism 4, an injector bracket 5, a push plate 7, a push rod 10 and a press plate 11;
the ice bath barrel 3 is placed on the base 1, the blood bag shaking mechanism 4 is arranged above the ice bath barrel 3, the top end of a rocker 4.3 of the blood bag shaking mechanism 4 is rotatably connected with the fixed frame 2, and a shell of a motor 4.6 of the blood bag shaking mechanism 4 is fixedly connected with the fixed frame 2; an injector bracket 5 is arranged above the blood bag shaking mechanism 4, two ends of the injector bracket 5 are fixedly connected with the fixed frame 2, and a plurality of placing holes 6 matched with the injector 14 in size are formed in the injector bracket 5; a push plate 7 is arranged above the injector support 5, two ends of the push plate 7 are fixedly provided with slide blocks 9, the opposite inner side of the fixed frame 2 above the injector support 5 is provided with a slide groove 8 matched with the slide block 9, and the slide block 9 is arranged in the slide groove 8 in a sliding manner; a through hole 12 is arranged on the fixing frame 2 right above the push plate 7, the bottom end of the push rod 10 is fixedly connected with the push plate 7, and the top end of the push rod 10 penetrates through the through hole 12 to be fixedly connected with the press plate 11.
The blood bag shaking mechanism 4 comprises a rocker 4.3, a clamping frame 4.1, a clamping plate 4.4, a limiting card 4.5, a motor 4.6, a speed reducer 4.7 and a cam 4.8; the bottom end of the rocker 4.3 is fixedly connected with the clamping frame 4.1, the output shaft of the motor 4.6 is in transmission connection with the input end of the speed reducer 4.7, the output shaft of the speed reducer 4.7 is fixedly provided with a cam 4.8, and the edge of the cam 4.8 is abutted against the rocker 4.3; when motor 4.6 drove cam 4.8 pivoted, cam 4.8 can drive rocker 4.3 and sway, and then drive blood bag 15 on the holding frame 4.1 and sway, play the effect of the abundant mixing of hematopoietic stem cell in freezing stock liquid in the blood bag 15 and the blood bag 15 of will injecting, it is even to need not artifical manual shaking, the work load has been alleviateed, and rock the frequency front-back unanimity, it is better to shake even effect, make the protective agent fully play a role, avoid the stem cell to receive the damage.
The clamping frame 4.1 is fixedly provided with clamping grooves 4.2 in the vertical direction, the number of the clamping grooves is equal to that of the placing holes 6, one side of the clamping frame 4.1 provided with the clamping grooves 4.2 is provided with a clamping plate 4.4, one end of the clamping plate 4.4 is rotatably connected with the clamping frame 4.1, the clamping frame 4.1 opposite to the other end of the clamping plate 4.4 is integrally provided with a limiting card 4.5, and the other end of the clamping plate 4.4 is movably connected with the limiting card 4.5 in a clamping mode.
This embodiment still includes the electric cylinder 13 of vertical setting, and electric cylinder 13's cylinder body and mount 2 fixed connection, electric cylinder 13's piston rod and push pedal 7 fixed connection.
When the device is used, firstly, the injector 14 filled with the frozen stock solution passes through the placing hole 6 and is erected on the injector bracket 5, then the self-provided fixing ring at the opening of the blood bag 15 is clamped in the clamping groove 4.2 on the clamping bracket 4.1, then the clamping plate 4.4 is clamped in the limiting clamp 4.5, and the blood bag 15 cannot be separated from the clamping bracket 4.1 under the action of the clamping plate 4.4; the needle port of the syringe 14 is connected to the blood line 16 of the blood bag 15. In this embodiment, the frozen liquid can be added to a plurality of blood bags 15 at the same time, and fig. 1 shows a use state in which the frozen liquid is added to only one blood bag 15. The pressing plate 11 can be manually pressed, so that the push plate 7 can simultaneously push the injector 14 to realize synchronous injection, the push plate 7 can also be pushed by the electric cylinder, the push plate 7 can be ensured to push the injector 14 at a constant speed, the frozen stock solution can be ensured to be added at a constant speed, the experimental requirement can be met in the adding process, and the umbilical cord blood hematopoietic stem cells can be reliably and effectively stored; in addition, the blood bag 15 is always arranged in the ice bath barrel 3, so that the blood bag 15 can be maintained at a constant temperature of about 4 ℃, and the cell activity of the umbilical cord blood hematopoietic stem cells is further ensured.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (3)
1. The frozen stock solution adding device in the cord blood hematopoietic stem cell separation process is characterized by comprising a base, a fixing frame fixedly connected with the base, an ice bath barrel, a blood bag shaking mechanism, an injector support, a push plate, a push rod and a press plate;
the ice bath barrel is placed on the base, the blood bag shaking mechanism is arranged above the ice bath barrel, the top end of a rocker of the blood bag shaking mechanism is rotatably connected with the fixing frame, and a shell of a motor of the blood bag shaking mechanism is fixedly connected with the fixing frame; the injector support is arranged above the blood bag shaking mechanism, two ends of the injector support are fixedly connected with the fixing frame, and a plurality of placing holes matched with the injector in size are formed in the injector support; the push plate is arranged above the injector support, sliding blocks are fixedly arranged at two ends of the push plate, sliding grooves matched with the sliding blocks are formed in the opposite inner sides of the fixing frame above the injector support, and the sliding blocks are arranged in the sliding grooves in a sliding mode; a through hole is formed in the fixing frame right above the push plate, the bottom end of the push rod is fixedly connected with the push plate, and the top end of the push rod penetrates through the through hole and is fixedly connected with the pressing plate.
2. The cryopreservation liquid adding device in the cord blood hematopoietic stem cell separation process according to claim 1, wherein the blood bag shaking mechanism comprises the rocker, a clamping frame, a clamping plate, a limiting card, the motor, a speed reducer and a cam;
the bottom end of the rocker is fixedly connected with the clamping frame, an output shaft of the motor is in transmission connection with an input end of the speed reducer, the output shaft of the speed reducer is fixedly provided with the cam, and the edge of the cam is abutted to the rocker;
the clamping frame is fixedly provided with clamping grooves in the vertical direction, the number of the clamping grooves is equal to that of the placing holes, the clamping plate is arranged on one side, provided with the clamping grooves, of the clamping frame, one end of the clamping plate is rotatably connected with the clamping frame, a limiting card is integrally arranged on the clamping frame, the position of the limiting card is opposite to that of the other end of the clamping plate, and the other end of the clamping plate is movably clamped in the limiting card.
3. The frozen stock solution adding device in the cord blood hematopoietic stem cell separation process according to claim 1, further comprising an electric cylinder vertically arranged, wherein a cylinder body of the electric cylinder is fixedly connected with the fixing frame, and a piston rod of the electric cylinder is fixedly connected with the push plate.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921211802.1U CN210526908U (en) | 2019-07-29 | 2019-07-29 | Frozen stock solution adding device in umbilical cord blood hematopoietic stem cell separation process |
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| CN201921211802.1U CN210526908U (en) | 2019-07-29 | 2019-07-29 | Frozen stock solution adding device in umbilical cord blood hematopoietic stem cell separation process |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111905200A (en) * | 2020-08-19 | 2020-11-10 | 南京市儿童医院 | Regulation and control type hematology department blood transfusion auxiliary device |
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- 2019-07-29 CN CN201921211802.1U patent/CN210526908U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111905200A (en) * | 2020-08-19 | 2020-11-10 | 南京市儿童医院 | Regulation and control type hematology department blood transfusion auxiliary device |
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