CN210514083U - Rapid detection device of up-conversion luminescence immunoassay analyzer - Google Patents
Rapid detection device of up-conversion luminescence immunoassay analyzer Download PDFInfo
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- CN210514083U CN210514083U CN201921408969.7U CN201921408969U CN210514083U CN 210514083 U CN210514083 U CN 210514083U CN 201921408969 U CN201921408969 U CN 201921408969U CN 210514083 U CN210514083 U CN 210514083U
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Abstract
The utility model relates to a fast detection device of an up-conversion luminescence immunoassay analyzer, a light path system of the fast detection device comprises an excitation light source, a shaping lens, a first dichroic mirror, a light homogenizing reflection device, a narrow-band light filter and a signal collector, wherein the centers of the excitation light source, the shaping lens and the first dichroic mirror are all on the same horizontal plane; and a reagent card horizontally arranged is arranged right below the center of the light uniformizing reflection device, and the light uniformizing reflection device and the reagent card form a third optical axis. The light path system arranges the excitation light source and the signal collector on the same horizontal plane, and the whole height of the light path is reduced. The utility model discloses furthest improves and detects the flux, accords with the needs of the short-term test's of instantaneous inspection, uses the static scheme of shooing, furthest's improvement detection speed.
Description
Technical Field
The utility model relates to a medical treatment check out test set technical field especially relates to a go up quick detection device of luminous immunoassay appearance.
Background
In the prior art, the up-conversion luminescence immunoassay analyzer generally adopts motor motion to position the position of a reagent card, and belongs to the field of dynamic scanning image detection. The up-conversion luminescence immunoassay analyzer adopts a plurality of LED light sources as excitation light sources, or adopts a scheme of fixing the excitation light sources, the reagent card and the image acquisition system. The image detection is dynamically scanned, the movement time of a movement mechanism during scanning influences the detection flux, and the flux is generally calculated according to the Test quantity per hour (Test/h). The multiple LED light sources are used because the energy of a single LED light source is insufficient, but the multiple LED light sources are complex in structure and high in positioning accuracy requirement, and the detection result is influenced when one LED light source fails. The scheme of fixing an excitation light source, a reagent card and an image acquisition system is adopted, although no movement mechanism exists, the method belongs to static photographing, laser spots are not shaped, the coverage area of the spots is small, and the spots are not enough to cover the detection of a multi-band chromatographic strip (a reagent strip of a general immunochromatography method is a T-band and a C-band, and the detection of a plurality of T-bands and a C-band is carried out in the market).
Therefore, it is desirable to provide a rapid detection device for an up-conversion luminescence immunoassay analyzer, which can improve the detection speed and realize rapid detection.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model aims at providing a go up quick detection device of luminescence immunoassay appearance of revolving, save the motion, avoid the trouble that the motion probably brought, through optical path system's design, use the static scheme of shooing, improve detection speed, realize short-term test.
In order to solve the technical problem, the utility model adopts the following technical proposal:
a rapid detection device of an up-conversion luminescence immunoassay analyzer comprises a light path system, wherein the light path system comprises an excitation light source, a shaping lens, a first dichroic mirror, a light homogenizing reflecting device, a narrow-band filter and a signal collector, the centers of the excitation light source, the shaping lens and the first dichroic mirror are all in the same horizontal plane, the excitation light source, the shaping lens and the first dichroic mirror are sequentially arranged to form a first optical axis, the light homogenizing reflecting device, the first dichroic mirror, the narrow-band filter and the signal collector are sequentially arranged to form a second optical axis, and the first optical axis is vertical to the second optical axis; the first dichroic mirror, the first optical axis and the second optical axis form an angle of 45 degrees respectively, the light uniformization reflecting device and the horizontal plane form an angle of 45 degrees, and the projection of the vertical central line of the light uniformization reflecting device on the horizontal plane and the second optical axis are on the same straight line; and a reagent card horizontally arranged is arranged right below the center of the light uniformizing reflection device, and the light uniformizing reflection device and the reagent card form a third optical axis.
Preferably, the light homogenizing reflecting device comprises a second dichroic mirror, a light homogenizing plate and a total reflecting mirror which are sequentially combined.
Preferably, the first dichroic mirror is totally reflective for light having a wavelength of 980nm and totally transmissive for light having a wavelength of 540 nm.
Preferably, the second dichroic mirror is fully transmissive for light having a wavelength of 980nm and fully reflective for light having a wavelength of 540 nm.
Preferably, the material of the light homogenizing plate is an engineering plastic film, an organic glass plate or a quartz glass plate, and the outer surface of the light homogenizing plate is provided with an atomizing layer. The principle of the light homogenizing plate is diffuse reflection, and the function of the light homogenizing plate is to homogenize light passing through the light homogenizing plate.
Preferably, the excitation light source is a semiconductor laser diode or a high-power light emitting diode, and the wavelength of the emergent light of the excitation light source is 980 nm. The semiconductor laser diode has the characteristics of good monochromaticity and small divergence angle compared with the light-emitting diode. The use of leds is generally powerful and there is sufficient margin of power that can be allocated to the homogenizing plate to achieve spot homogenization. The shaping lens is used for amplifying and shaping the banded facula of the emergent light of the excitation light source.
Preferably, the signal collector is a cmos or CCD sensor. Either cmos or CCD sensors are used. The photoelectric diode has the advantages of high sensitivity and higher practicability compared with a photomultiplier by replacing the traditional photodiode and a traditional photocell. The narrow-band filter is used for eliminating stray light with other wavelengths.
Preferably, the reagent card is provided with up-conversion luminescent particles capable of exciting light with the wavelength of 540 nm. Using the principle of up-conversion luminescence, 980nm light having a longer wavelength is used to excite 540nm light having a shorter wavelength.
The light path shaping method of the rapid detection device of the up-conversion luminescence immunoassay analyzer comprises the following steps:
an excitation light source is used for providing emergent light with the wavelength of 980nm, the emergent light is primarily shaped on a first optical axis through a shaping lens to amplify and shape a banded light spot of the emergent light, the 980nm light is totally reflected through a first dichroic mirror and is totally transmitted at 540nm, the 980nm light is homogenized through a light homogenizing plate after being totally transmitted through a second dichroic mirror on a second optical axis, and is secondarily homogenized through a light homogenizing plate after being secondarily reflected by a total reflecting mirror and then is secondarily transmitted through the second dichroic mirror to form a light spot with a certain shape;
the shaped light spot vertically irradiates the reagent card downwards on a third optical axis to excite a 540nm visible light, the 540nm visible light vertically irradiates the second dichroic mirror upwards, is totally reflected by the second dichroic mirror on the second optical axis, is totally transmitted by the first dichroic mirror, and is received by the signal collector after being filtered by the narrow-band filter.
The utility model has the advantages as follows:
the utility model discloses owing to adopted above technical scheme, light path system will excite light source and signal collector overall arrangement at same horizontal plane, and the light path is whole highly reduces, can reduce the height of the luminous immunoassay appearance of revolving, and the light path is whole more flat. The utility model discloses a but the high-speed checking device furthest improves and detects the flux, accords with the needs of the high-speed check of real-time supervision, has saved motion, has avoided the trouble that motion probably brought, uses the static scheme of shooing, furthest's improvement detection speed.
The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical means of the present invention more clearly understood and to make the technical means more comprehensible, and to make the above and other objects, technical features, and advantages of the present invention easier to understand, one or more preferred embodiments are listed below, and the following detailed description is given with reference to the accompanying drawings.
Drawings
One or more embodiments are illustrated by way of example in the accompanying drawings, which correspond to the figures in which like reference numerals refer to similar elements and which are not to scale unless otherwise specified.
Fig. 1 shows a schematic structural diagram of a rapid detection device of an up-conversion luminescence immunoassay analyzer according to the present invention.
Fig. 2 shows a schematic diagram of an optical path system of a rapid detection device of an up-conversion luminescence immunoassay analyzer according to the present invention.
Fig. 3 shows a schematic diagram of a light path shaping method of a rapid detection device of an up-conversion luminescence immunoassay analyzer according to the present invention.
Description of the main reference numerals:
1-excitation light source, 2-shaping lens, 3-first dichroic mirror, 4-second dichroic mirror, 5-light homogenizing plate, 6-total reflection mirror, 7-reagent card, 8-narrow band filter and 9-signal collector.
Detailed Description
The following detailed description of the present invention is provided in conjunction with the accompanying drawings, but it should be understood that the scope of the present invention is not limited by the following detailed description.
Throughout the specification and claims, unless explicitly stated otherwise, the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element or component but not the exclusion of any other element or component.
Spatially relative terms, such as "below," "lower," "upper," "above," "upper," and the like, may be used herein for ease of description to describe one element or feature's relationship to another element or feature in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the object in use or operation in addition to the orientation depicted in the figures. For example, if the items in the figures are turned over, elements described as "below" or "beneath" other elements or features would then be oriented "above" the elements or features. Thus, the exemplary term "below" can encompass both an orientation of below and above. The article may be otherwise oriented (rotated 90 degrees or at other orientations) and the spatially relative terms used herein should be interpreted accordingly.
As shown in fig. 1 and fig. 2, a rapid detection device of an up-conversion luminescence immunoassay analyzer includes a light path system, where the light path system includes an excitation light source 1, a shaping lens 2, a first dichroic mirror 3, a uniform light reflection device, a narrow-band optical filter 8, and a signal collector 9, where the centers of the excitation light source 1, the shaping lens 2, and the first dichroic mirror 3 are all on the same horizontal plane, and the first optical axis is formed by sequentially arranging the excitation light source 1, the shaping lens 2, and the first dichroic mirror 3.
The excitation light source 1 is a semiconductor laser diode or a high-power light emitting diode, and the wavelength of emergent light of the excitation light source 1 is 980 nm. The semiconductor laser diode has the characteristics of good monochromaticity and small divergence angle compared with the light-emitting diode. The use of leds is generally powerful and has sufficient margin to allow for distribution to the homogenizing plate 5 to achieve spot homogenization. The shaping lens 2 is used for enlarging and shaping the band-shaped light spot of the outgoing light of the excitation light source 1. The first dichroic mirror 3 totally reflects light with a wavelength of 980nm and totally transmits light with a wavelength of 540 nm.
The uniform light reflection device, the first dichroic mirror 3, the narrow-band optical filter 8 and the signal collector 9 are sequentially arranged to form a second optical axis.
The uniform light reflection device comprises a second dichroic mirror 4, a uniform light plate 5 and a total reflection mirror 6 which are sequentially compounded. The material of the light homogenizing plate 5 is an engineering plastic film, an organic glass plate or a quartz glass plate, and the outer surface of the light homogenizing plate 5 is provided with an atomizing layer. The principle of the light homogenizing plate 5 is diffuse reflection, and the function is to homogenize the light passing through the light homogenizing plate 5. The light homogenizing plate 5 may also be placed on the excitation light source 1, the shaping lens 2, or the first beam splitter 3. The second dichroic mirror 4 is totally transmissive for light with a wavelength of 980nm and totally reflective for light with a wavelength of 540 nm. The narrow-band filter 8 functions to eliminate stray light of other wavelengths. The signal collector 9 is a cmos or CCD sensor. Either cmos or CCD sensors are used. The photoelectric diode has the advantages of high sensitivity and higher practicability compared with a photomultiplier by replacing the traditional photodiode and a traditional photocell.
The first optical axis and the second optical axis are perpendicular.
The first dichroic mirror 3 forms an angle of 45 degrees with the first optical axis and the second optical axis respectively. The first dichroic mirror 3 is perpendicular to the horizontal plane.
The even light reflection device and the horizontal plane form an angle of 45 degrees, and the projection of the vertical central line of the even light reflection device on the horizontal plane and the second optical axis are on the same straight line.
And a reagent card 7 which is horizontally arranged is arranged right below the center of the light uniformizing reflection device, and the light uniformizing reflection device and the reagent card 7 form a third optical axis. The reagent card 7 is provided with up-conversion luminescent particles capable of exciting light with the wavelength of 540 nm. Using the principle of up-conversion luminescence, 980nm light having a longer wavelength is used to excite 540nm light having a shorter wavelength.
As shown in fig. 3, the optical path shaping method of the rapid detection device using the above-mentioned up-conversion luminescence immunoassay analyzer includes:
an excitation light source 1 is used for providing emergent light with the wavelength of 980nm, the emergent light is primarily shaped on a first optical axis through a shaping lens 2 to amplify and shape a banded light spot of the emergent light, the 980nm light is totally reflected through a first dichroic mirror 3, the 540nm light is totally transmitted, the 980nm light is homogenized through a light homogenizing plate 5 after being totally transmitted through a second dichroic mirror 4 on a second optical axis, and then is secondarily homogenized through a light homogenizing plate 5 after being secondarily reflected through a total reflecting mirror 6, and then is secondarily transmitted through the second dichroic mirror 4 to form a light spot with a certain shape;
the shaped light spot vertically irradiates the reagent card 7 downwards on a third optical axis to excite a 540nm visible light, the 540nm visible light vertically irradiates the second dichroic mirror 4 upwards, is totally reflected by the second dichroic mirror 4 on the second optical axis, is totally transmitted by the first dichroic mirror 3, is filtered by the narrow-band filter 8 and is received by the signal collector 9.
The utility model discloses a fast detection device of up-conversion luminescence immunoassay appearance can furthest's improvement detection speed, gets rid of the reaction time of outer reagent, and the plug-in card after possible reacting is surveyed promptly.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. Any simple modifications, equivalent changes and modifications made to the above exemplary embodiments shall fall within the scope of the present invention.
Claims (8)
1. A rapid detection device of an up-conversion luminescence immunoassay analyzer is characterized by comprising a light path system, wherein the light path system comprises an excitation light source (1), a shaping lens (2), a first dichroic mirror (3), a light homogenizing reflecting device, a narrow-band filter (8) and a signal collector (9), the centers of the excitation light source (1), the shaping lens (2) and the first dichroic mirror (3) are all on the same horizontal plane, the excitation light source (1), the shaping lens (2) and the first dichroic mirror (3) are sequentially arranged to form a first optical axis, the light homogenizing reflecting device, the first dichroic mirror (3), the narrow-band filter (8) and the signal collector (9) are sequentially arranged to form a second optical axis, and the first optical axis is vertical to the second optical;
the first dichroic mirror (3) forms an angle of 45 degrees with the first optical axis and the second optical axis respectively, the uniform light reflecting device forms an angle of 45 degrees with the horizontal plane, and the projection of the vertical central line of the uniform light reflecting device on the horizontal plane is on the same straight line with the second optical axis;
and a reagent card (7) horizontally arranged is arranged right below the center of the light uniformizing reflection device, and the light uniformizing reflection device and the reagent card (7) form a third optical axis.
2. The rapid detection device of the up-conversion luminescence immunoassay analyzer according to claim 1, wherein the light homogenizing reflecting device comprises a second dichroic mirror (4), a light homogenizing plate (5) and a total reflection mirror (6) which are combined in sequence.
3. The rapid detection device of the up-conversion luminescence immunoassay analyzer according to claim 1, wherein the first dichroic mirror (3) is totally reflective for 980nm wavelength light and totally transmissive for 540nm wavelength light.
4. The rapid detection device of the up-conversion luminescence immunoassay analyzer according to claim 2, wherein the second dichroic mirror (4) is totally transmissive to the light with the wavelength of 980nm and totally reflective to the light with the wavelength of 540 nm.
5. The rapid detection device of the up-conversion luminescence immunoassay analyzer according to claim 2, wherein the light homogenizing plate (5) is made of an engineering plastic film, an organic glass plate or a quartz glass plate, and the outer surface of the light homogenizing plate (5) is provided with an atomizing layer.
6. The rapid detection device of the up-conversion luminescence immunoassay analyzer according to claim 1, wherein the excitation light source (1) is a semiconductor laser diode or a high-power light emitting diode, and the wavelength of the emergent light of the excitation light source (1) is 980 nm.
7. The rapid detection device of the up-conversion luminescence immunoassay analyzer of claim 1, wherein the signal collector (9) is a COMS or CCD sensor.
8. The rapid detection device of the up-conversion luminescence immunoassay analyzer according to claim 1, wherein the reagent card (7) is provided with up-conversion luminescence particles capable of exciting light with a wavelength of 540 nm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921408969.7U CN210514083U (en) | 2019-08-28 | 2019-08-28 | Rapid detection device of up-conversion luminescence immunoassay analyzer |
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| CN201921408969.7U CN210514083U (en) | 2019-08-28 | 2019-08-28 | Rapid detection device of up-conversion luminescence immunoassay analyzer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110411989A (en) * | 2019-08-28 | 2019-11-05 | 热景（廊坊）生物技术有限公司 | A kind of device for fast detecting its optical path shaping methods of upper forwarding light immunity analysis instrument |
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2019
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110411989A (en) * | 2019-08-28 | 2019-11-05 | 热景（廊坊）生物技术有限公司 | A kind of device for fast detecting its optical path shaping methods of upper forwarding light immunity analysis instrument |
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Address after: 065000 No. 70, Guandong Road, Langfang Development Zone, Langfang City, Hebei Province Patentee after: HOTGEN (LANGFANG) BIOTECH Co.,Ltd. Address before: 065000 6th floor, No. 6 plant of Wantong Industrial Co., Ltd., West Chuangye Road, Langfang Development Zone, Langfang City, Hebei Province Patentee before: HOTGEN (LANGFANG) BIOTECH Co.,Ltd. |