CN210506341U - Device for changing culture solution in liquid culture of edible fungi - Google Patents
Device for changing culture solution in liquid culture of edible fungi Download PDFInfo
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- CN210506341U CN210506341U CN201920928584.7U CN201920928584U CN210506341U CN 210506341 U CN210506341 U CN 210506341U CN 201920928584 U CN201920928584 U CN 201920928584U CN 210506341 U CN210506341 U CN 210506341U
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Abstract
The utility model relates to a trade device of culture solution in domestic fungus liquid culture, including a erlenmeyer flask, the opening has been seted up at this erlenmeyer flask top, and inlet and liquid outlet have been seted up respectively to upper portion position in the erlenmeyer flask outer peripheral face, and inlet and liquid outlet department are connected with feed liquor pipe and drain pipe respectively, feed liquor pipe, drain pipe and erlenmeyer flask be integrated into one piece setting, and the device that trades culture solution in this domestic fungus liquid culture simple structure is reasonable, and is safe convenient.
Description
Technical Field
The utility model relates to a device for changing culture solution in edible fungus liquid culture.
Background
When the liquid strain of the edible fungi in the laboratory is fermented, different culture media are often required to be replaced in the fermentation process. Previously, the medium was changed by filtering the mycelium pellets from a flask and then washing them before inoculating them into the new medium. The operation has high pollution rate and complex operation.
SUMMERY OF THE UTILITY MODEL
In view of the not enough of prior art, the utility model aims to solve the technical problem that a device of trading culture solution in domestic fungus liquid culture is provided, not only rational in infrastructure, the simple operation moreover.
In order to solve the technical problem, the technical scheme of the utility model is that: the utility model provides a device of trading culture solution in domestic fungus liquid culture, includes a erlenmeyer flask, and the opening has been seted up at this erlenmeyer flask top, and inlet and liquid outlet have been seted up respectively to upper portion position in the erlenmeyer flask outer peripheral face, and inlet and liquid outlet department are connected with feed liquor pipe and drain pipe respectively, feed liquor pipe, drain pipe and erlenmeyer flask set up for integrated into one piece.
Furthermore, feed liquor pipe, drain pipe and erlenmeyer flask all are in on same vertical plane, and the feed liquor pipe is located the opposite one side of drain pipe promptly.
Furthermore, the liquid inlet pipe and the liquid outlet pipe both deviate outwards along the central axis of the conical flask by 45 degrees and extend upwards in an inclined manner.
Furthermore, the aperture of the opening is 3cm, the aperture of the liquid inlet is 2cm, the aperture of the liquid outlet is 1cm, and the horizontal positions of the top ends of the liquid inlet pipe and the liquid outlet pipe are lower than the horizontal position of the top of the conical flask.
Furthermore, the opening is plugged with a ventilation plug, and the upper ends of the liquid inlet pipe and the liquid outlet pipe are plugged with silica gel plugs.
Furthermore, a filter screen is arranged at the position of the liquid outlet.
Compared with the prior art, the utility model discloses following beneficial effect has: through the design of feed liquor pipe and drain pipe for it can go on only to change the culture solution in this isolated plant, and whole process mycelium ball need not to leave the erlenmeyer flask, and easy operation, convenient and fast, and difficult pollution, and do not influence the fermentation of shaking the bottle normally, trades the device of culture solution in this edible mushroom liquid culture, easy operation, effectual reduction is to the pollution of mycelium ball.
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
Drawings
Fig. 1 is a schematic structural diagram of an embodiment of the present invention.
In the figure: 1-conical flask, 2-opening, 3-liquid inlet, 4-liquid outlet, 5-liquid inlet pipe, 6-liquid outlet pipe, 7-air-permeable plug, 8-silica gel plug and 9-filter screen.
Detailed Description
As shown in figure 1, a device for changing culture solution in liquid culture of edible fungi comprises a conical flask 1, wherein an opening 2 is formed in the top of the conical flask, a liquid inlet 3 and a liquid outlet 4 are respectively formed in the middle upper part of the outer peripheral surface of the conical flask, a liquid inlet pipe 5 and a liquid outlet pipe 6 are respectively connected to the liquid inlet and the liquid outlet, and the liquid inlet pipe, the liquid outlet pipe and the conical flask are integrally formed.
The embodiment of the utility model provides an in, feed liquor pipe, drain pipe and erlenmeyer flask all are in on the same vertical plane, and the feed liquor pipe is located opposite one side of drain pipe promptly.
In the embodiment of the utility model, feed liquor pipe and drain pipe all extend up along the outside skew 45 degrees slants of erlenmeyer flask axis.
The embodiment of the utility model provides an in, the opening bore is 3cm, and the inlet bore is 2cm, and the liquid outlet bore is 1cm, feed liquor pipe and drain pipe top place horizontal position all are less than erlenmeyer flask top horizontal plane position.
In the embodiment of the utility model, the opening part stopper has ventilative stopper 7, and feed liquor pipe and drain pipe upper end all are filled with silica gel stopper 8.
In the embodiment of the utility model, the liquid outlet position is provided with filter screen 9.
The utility model discloses theory of operation of embodiment: when the inoculation for the first time, with the edible mushroom bacterial follow the opening part and insert, because the opening is great and adopt ventilative stopper, oxygen can get into the erlenmeyer flask and carry out aerobic fermentation, in the culture solution is changed to needs, in superclean bench, open the silica gel stopper of drain pipe earlier, pour out old culture solution through the drain pipe, the mycelium ball then is stayed in the erlenmeyer flask because of the filter screen. At the moment, the silica gel plug of the liquid outlet pipe is plugged, then the silica gel plug of the liquid inlet pipe is opened, the new culture solution is added through the liquid inlet pipe, and then the silica gel plug of the liquid inlet pipe is plugged. The mycelium pellet does not need to leave the conical flask in the whole process, and the method is simple to operate, convenient and fast, is not easy to pollute and does not influence normal flask shaking fermentation. The device bottle has the characteristics of simple operation, pollution reduction and rapid culture solution replacement midway, and is suitable for the research of liquid fermentation of edible fungi and the like in a laboratory.
The utility model is not limited to the above best mode, and any person can obtain the device for changing the culture solution in the liquid culture of other various edible fungi under the teaching of the utility model. All the equivalent changes and modifications made according to the claims of the present invention shall fall within the scope of the present invention.
Claims (5)
1. The utility model provides a trade device of culture solution in domestic fungus liquid culture which characterized in that: the liquid inlet and the liquid outlet are respectively connected with a liquid inlet pipe and a liquid outlet pipe, and the liquid inlet pipe, the liquid outlet pipe and the conical bottle are integrally formed; and a filter screen is arranged at the liquid outlet.
2. The apparatus for changing culture solution in liquid culture of edible fungi according to claim 1, wherein: the liquid inlet pipe, the liquid outlet pipe and the conical bottle are all located on the same vertical plane, namely the liquid inlet pipe is located on one side opposite to the liquid outlet pipe.
3. The apparatus for changing culture solution in liquid culture of edible fungi according to claim 1, wherein: the liquid inlet pipe and the liquid outlet pipe both deviate outwards along the central axis of the conical flask by 45 degrees and extend upwards in an inclined manner.
4. The apparatus for changing culture solution in liquid culture of edible fungi according to claim 1, wherein: the aperture of the opening is 3cm, the aperture of the liquid inlet is 2cm, the aperture of the liquid outlet is 1cm, and the horizontal positions of the top ends of the liquid inlet pipe and the liquid outlet pipe are lower than the horizontal position of the top of the conical flask.
5. The apparatus for changing culture solution in liquid culture of edible fungi according to claim 4, wherein: the opening part is plugged with a ventilation plug, and the upper ends of the liquid inlet pipe and the liquid outlet pipe are plugged with silica gel plugs.
Priority Applications (1)
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CN201920928584.7U CN210506341U (en) | 2019-06-20 | 2019-06-20 | Device for changing culture solution in liquid culture of edible fungi |
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CN201920928584.7U CN210506341U (en) | 2019-06-20 | 2019-06-20 | Device for changing culture solution in liquid culture of edible fungi |
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CN210506341U true CN210506341U (en) | 2020-05-12 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113667601A (en) * | 2021-07-28 | 2021-11-19 | 江苏谱新生物医药有限公司 | Device and method for continuous culture and sampling of cells |
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2019
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113667601A (en) * | 2021-07-28 | 2021-11-19 | 江苏谱新生物医药有限公司 | Device and method for continuous culture and sampling of cells |
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