CN210487788U - Chromatography detection card - Google Patents
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- CN210487788U CN210487788U CN201921266361.5U CN201921266361U CN210487788U CN 210487788 U CN210487788 U CN 210487788U CN 201921266361 U CN201921266361 U CN 201921266361U CN 210487788 U CN210487788 U CN 210487788U
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Abstract
The utility model discloses a chromatography detects card, including shell, test paper card, the supporting device who is used for bearing test paper card, supporting device detachably installs in the shell, and the upper surface of shell is provided with the inspection hole, and inspection line and quality control line on the nitrocellulose membrane of the test paper card on inspection hole and the supporting device are corresponding, are provided with the patchhole that is used for supporting device business turn over shell on the side of shell. The utility model discloses a chromatography detects the card, and the test paper card is passed in and out the shell by the side of shell, and it is simple to change the test paper card like this, saves time, makes detection efficiency uprise.
Description
Technical Field
The utility model relates to a detecting system especially relates to a chromatography detects card.
Background
Foot-and-Mouth Disease (FMD) is a major animal epidemic Disease caused by FMDV (Foot-and-Mouth Disease Virus) and mainly caused by the Disease of pig, cattle and sheep. The disease has multiple propagation ways, high speed and wide epidemic areas, once the disease is attacked, the disease not only causes huge economic loss, but also can cause severe political influence, is highly concerned by all countries in the world, and is called the political economic disease.
In the prior art, the detection of the A-type foot-and-mouth disease antibody still mainly takes a qualitative/quantitative ELISA kit as a main part, and provides an important technical support for the prevention and control of the foot-and-mouth disease in China, but the technology still has a plurality of defects and shortcomings, firstly, the operation is complex, and the labor intensity is high; secondly, special laboratories and professional personnel are required for operation; moreover, the experiment time is long, and the result is slow. Although the colloidal gold test paper card for detecting the foot-and-mouth disease antibody is convenient to detect, no professional technical personnel and working environment are needed; however, the detection sensitivity is low, and the detection range is narrow, and can only be qualitative. In the prior art, a detection technology for scanning a chromatography detection card by using a chromatography scanner is available, but the conventional chromatography detection card has a complex structure, is troublesome in replacing a test paper card, and is easy to waste time, so that the detection efficiency is low.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to provide a higher chromatography detection card of detection efficiency.
The utility model discloses a chromatography detection card, which comprises a housing, a test paper card, a supporting device for supporting the test paper card, the supporting device is detachably installed in the housing, the test paper card comprises a bottom plate with pressure-sensitive adhesive, a sample pad, a marker pad, a nitrocellulose membrane and absorbent paper are sequentially pasted on the bottom plate, the marker pad is composed of a carrier base layer and a marker, foot-and-mouth disease virus epitope recombinant antigen is coated on the nitrocellulose membrane as a detection line, anti-chicken lgY antibody is coated on the nitrocellulose membrane as a quality control line, the marker is a mixture of fluorescent detection microspheres marked with the foot-and-mouth disease virus VP1 recombinant antigen and fluorescent quality control microspheres marked with the chicken lgY antibody, a sample dripping hole is arranged on the upper surface of the housing, the sample dripping hole corresponds to the sample pad of the test paper card on the supporting device, an observation hole is arranged on the upper surface of the housing, the observation hole corresponds to the cellulose membrane and the quality control line on the nitrocellulose membrane of the test paper card on the supporting device, the side of the shell is provided with an insertion hole for the supporting device to enter and exit the shell.
The utility model discloses a chromatography detects card, wherein, bearing device includes flat board, layer board and adjusting bolt, and the layer board is used for bearing test paper card, and the layer board sets up in dull and stereotyped top, and dull and stereotyped one end is passed through the connecting plate with the one end of layer board and is articulated together, and the dull and stereotyped other end is provided with the clearing hole, and adjusting bolt's double-screw bolt is passed behind the clearing hole from bottom to top and is interfered with the layer board to when adjusting bolt's double-screw bolt upwards precession, the double-screw bolt is with layer board jack-up, the.
The utility model discloses a chromatography detects card, wherein, is provided with the locking hole on bearing device's the flat board, and the inside of shell is provided with locking spring and ejector pin, and locking spring sets up in the one end of ejector pin for the other end that promotes the ejector pin stretches into in the dull and stereotyped locking hole, locks bearing device's flat board in the inside operating position of shell.
The utility model discloses a chromatography detects card, wherein, the inside of shell still is provided with the electro-magnet that is used for attracting locking spring or ejector pin and is used for making the supporting device outwards pop out the reset spring by the patchhole, and when the supporting device popped out, the electro-magnet circular telegram produced magnetic force, attracts locking spring or ejector pin, makes the ejector pin extract from the locking hole, and the supporting device pops out the shell from operating position under the pop-up force effect that reset spring applyed.
The utility model discloses a chromatography detects the card, and the test paper card is passed in and out the shell by the side of shell, and it is simple to change the test paper card like this, saves time, makes detection efficiency uprise.
Drawings
FIG. 1 is a schematic block diagram of a rapid quantitative detection system for foot-and-mouth disease virus antibodies;
FIG. 2 is a front view of a schematic view of a test card;
FIG. 3 is a front view of a schematic of the structure of the chromatography detection card;
FIG. 4 is a top view of a schematic of the structure of the chromatography detection card;
FIG. 5 is a top view of a schematic diagram of a test card;
fig. 6 is a top sectional view of the structural schematic of the housing.
Detailed Description
As shown in fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, and fig. 6, the rapid quantitative detection system for foot-and-mouth disease virus antibodies comprises:
chromatography detection card;
the chromatography scanner is used for scanning the chromatography detection card to obtain detection data;
a receiving end connected with the chromatographic scanner for receiving and analyzing the detection data of the chromatographic scanner,
the chromatography detection card comprises a shell 40, a test paper card 100 and a supporting device 20 for supporting the test paper card, wherein the supporting device is detachably installed in the shell 40, the test paper card 100 comprises a bottom plate 1 with pressure-sensitive adhesive, a sample pad 2, a marker pad 3, a nitrocellulose membrane 4 and absorbent paper 5 are sequentially adhered on the bottom plate, the marker pad consists of a carrier base layer and a marker, the nitrocellulose membrane is coated with foot-and-mouth disease virus epitope recombinant antigen to form a detection line 6, the nitrocellulose membrane is coated with rabbit anti-chicken lgY antibody to form a quality control line 7, the marker is a mixture of fluorescent detection microspheres marked with the foot-and-mouth disease virus VP1 recombinant antigen and fluorescent quality control microspheres marked with the chicken lgY antibody, a sample dripping hole 10 is formed in the upper surface of the shell and corresponds to the sample pad of the test paper card on the supporting device, an observation hole 11 is formed in the upper surface of the shell and corresponds to the detection line and the quality control line on the nitrocellulose membrane of the test paper card on the supporting device, the side of the housing is provided with an insertion hole 101 for the retainer to enter and exit the housing.
The utility model discloses a chromatography detects card, wherein, supporting device 20 includes dull and stereotyped 21, layer board 22 and adjusting bolt 23, the layer board is used for bearing test paper card 100, the layer board sets up in dull and stereotyped top and is parallel with the flat board, dull and stereotyped one end is passed through connecting plate 24 with the one end of layer board and is articulated together, dull and stereotyped other end is provided with clearing hole 25, adjusting bolt's double-screw bolt passes behind the clearing hole from bottom to top and interferes with the layer board, in order when adjusting bolt's double-screw bolt upwards precession, the double-screw bolt is with layer board jack-up, the relative dull and.
A receiving module, a data processing module, a storage module and a communication module are arranged in the receiving end, and the receiving module, the storage module and the communication module are all connected with the data processing module; the receiving module is used for receiving the detection data transmitted by the chromatographic scanner and transmitting the detection data to the data processing module; the data processing module analyzes data and transmits results to the storage module and the communication module.
The receiving end is internally provided with a display module, the display module is connected with the data processing module, and the display module is used for displaying the detection data and the detection result.
The flat plate of the supporting device is provided with a locking hole 31, the inside of the shell is provided with a locking spring 32 and an ejector rod 33, the locking spring is arranged at one end of the ejector rod and used for pushing the other end of the ejector rod to stretch into the locking hole of the flat plate so as to lock the flat plate of the supporting device at the working position inside the shell.
The housing 40 is also provided with an electromagnet 35 for attracting the locking spring or ram and a return spring 38 for ejecting the racking device from the insertion hole, and when the racking device is required to be ejected, the electromagnet is energized to generate magnetic force to attract the locking spring or ram to pull the ram out of the locking hole, and the racking device is ejected from the working position to the housing under the action of the ejection force exerted by the return spring.
The return springs 38 are three in number, the return springs 38 are disposed inside the housing 40, the return springs 38 are disposed inside the insertion holes, and the axes of the return springs are parallel to the flat plate.
The supporting device is used for supporting the test paper card, so that the test paper card can work in a more stable state, the locking spring 32, the ejector rod 33 and the electromagnet 35 form a set of locking ejection system, when the supporting device for supporting the test paper card is pushed into the shell 40 through the insertion hole 101, the supporting device is located at a working position, the electromagnet is powered off, the locking spring pushes the other end of the ejector rod to extend into the locking hole of the flat plate, so that the flat plate of the supporting device is locked at the working position in the shell, and the reset spring 38 is compressed; when the test paper card needs to be replaced, the adjusting bolt is screwed out downwards, the electromagnet is electrified to generate magnetic force to attract the locking spring or the ejector rod, the ejector rod is pulled out from the locking hole, and the supporting device is ejected out of the shell from the working position under the ejecting force applied by the reset spring.
The chromatographic scanner is used for carrying the chromatographic detection card and scanning the chromatographic detection card;
the chromatographic scanner is wirelessly connected with the receiving end;
the receiving end is connected with a cloud platform through the Internet, and the cloud platform is used for storing a standard curve and detection data of the detected A-type foot-and-mouth disease virus antibody;
the foot-and-mouth disease virus epitope recombinant antigen is a main antigen epitope on A-type foot-and-mouth disease virus VP1, and is expressed epitope recombinant protein.
The rabbit anti-chicken lgY antibody adopted by the quality control line on the nitrocellulose membrane is lgG of the rabbit anti-chicken lgY.
The receiving end is a mobile phone or a tablet computer with an internet surfing function.
Lanthanide fluorescence of lanthanide fluorescent microspheres labeled on biological materials refers to fluorescence generated by lanthanide elements Eu, Sm or Tb and β -diketone ligand.
The marker is a film formed by spraying lanthanide fluorescent detection microspheres and lanthanide fluorescent quality control microspheres on the carrier substrate.
The noun explains:
lgG of rabbit anti-chicken lgY: rabbit immunoglobulin G against chicken lgY;
chicken lgY antibody: chicken egg yolk antibodies;
coli competent cell BL21(DE3) pLysS: the most commonly used host strains for E.coli expression systems.
The rapid quantitative detection system for the foot-and-mouth disease virus antibody can realize the on-site rapid quantitative detection of the A-type foot-and-mouth disease virus antibody, provides a high-efficiency, sensitive and rapid monitoring means and technology for the prevention and control of the A-type foot-and-mouth disease in China, and has higher practical value and popularization value.
The chromatography detection card comprises a test paper card assembled in a shell 40, the test paper card comprises a bottom plate 1 made of a plastic material with a pressure-sensitive adhesive, a sample pad 2, a marker pad 3, a nitrocellulose membrane 4 and absorbent paper 5 are sequentially pasted on the bottom plate 1 from left to right, the right end of the sample pad 2 is lapped on the left end of the marker pad 3, the right end of the marker pad 3 is lapped on the left end of the nitrocellulose membrane 4, and the left end of the absorbent paper 5 is lapped on the right end of the nitrocellulose membrane 4. A detection line 6 is coated with an A-type foot-and-mouth disease virus epitope recombinant antigen on the nitrocellulose membrane 4, a quality control line 7 is coated with a rabbit anti-chicken lgY antibody, a sample dripping hole 10 is formed in the shell 10 corresponding to the sample pad 2, and an observation hole 11 is formed in the nitrocellulose membrane 4 corresponding to the detection line 6 and the quality control line 7.
The test card is held in the housing 10 with the sample pad 2 at the lower end and the bibulous paper 5 at the upper end. This kind of structure makes the sample velocity of flow that awaits measuring that drips into from sample drip injection hole 11 slow down, and the chromatography up slowly guarantees sufficient chromatography time, can effectively prevent that liquid sample from flowing too fast and influencing the testing result, avoids the sample too fast to flow into nitrocellulose membrane 4 and leads to the testing result failure. Because, if the flow rate of the sample to be detected is too high, insufficient chromatography is easily caused, and the detection result is further influenced. The utility model discloses a chromatography detects the card, when adjusting bolt's double-screw bolt upwards precession, the double-screw bolt with layer board jack-up, the relative dull and stereotyped tilt up of the other end of layer board, can adjust the angle of layer board slope through adjusting bolt promptly, strengthen the adaptability of chromatography detection card like this greatly.
The marker pad 3 is composed of a carrier substrate and a marker, the marker is a film formed by spraying lanthanide fluorescent detection microspheres and lanthanide fluorescent quality control microspheres on the carrier substrate, the marker is fluorescent detection microspheres marked with recombinant antigens of A-type foot-and-mouth disease virus VP1 protein and fluorescent quality control microspheres marked with chicken lgY antibodies, the lanthanide fluorescent of the lanthanide fluorescent microspheres marked on biological raw materials refers to the fluorescent generated by lanthanide element Eu, Sm or Tb and β -diketone ligands, the recombinant antigens of A-type foot-and-mouth disease virus epitopes are epitope antigens of A-type foot-and-mouth disease virus VP1, which are expressed epitope recombinant proteins, the rabbit anti-chicken lgY antibodies adopted by a quality control line on the cellulose nitrate film 4 are lgG of rabbit anti-chicken lgY, and the markers of A-type foot-and-mouth disease virus antibodies, A-type foot-and VP1 recombinant antigens in blood samples and the coated recombinant antigens of A-type foot-and-mouth disease virus epitopes on the cellulose nitrate film are used for detecting the pig, cattle, A-type foot-and other animal blood foot-and mouth disease viruses by a double-antigen sandwich method.
A receiving module, a data processing module, a storage module and a communication module are arranged in the receiving end, and the receiving module, the storage module and the communication module are all connected with the data processing module; the receiving module is used for receiving the data transmitted by the tomography scanner and transmitting the data to the data processing module; the data processing module analyzes data and transmits results to the storage module and the communication module; the communication module is connected with the cloud platform through the Internet. The receiving end is a mobile phone or a tablet computer with an internet surfing function.
The working principle of the rapid quantitative detection system for the foot-and-mouth disease virus antibody is as follows:
after a sample to be detected is pretreated, the sample to be detected is dripped into a sample dripping hole of the chromatography detection card, after chromatography is carried out for 15 minutes, the chromatography detection card is placed into a chromatography scanner for scanning, an excitation light source in the chromatography scanner emits excitation light to irradiate the chromatography detection card, so that fluorescent microspheres on the chromatography detection card are excited to generate fluorescence, the chromatography scanner collects fluorescence signal intensity values on the chromatography detection card point by point, and then the detection of the whole chromatography detection card is completed. The chromatography scanner transmits detection data to a receiving module of a receiving end through Bluetooth, the data processing module detects the data according to the fluorescence signal intensity of the chromatography detection card, the fluorescence signal intensity values of a quality control line and a detection line on the chromatography detection card are calculated, meanwhile, the data processing module obtains a standard curve of an A-type foot-and-mouth disease virus antibody of a detected sample from a cloud platform through the communication module and stores the standard curve in the storage module, the data processing module calculates the content of the A-type foot-and-mouth disease virus antibody in the detected sample according to the standard curve (the contrast relation between the fluorescence signal intensity values of the quality control line and the detection line and the standard curve), and displays the content, and detection results are stored in the storage module and the cloud platform.
Preparing a foot-and-mouth disease virus antibody detection standard curve:
6 parts of a calibration solution (containing A-type foot-and-mouth disease virus antibody standard) containing A-type foot-and-mouth disease virus antibodies are prepared, and the concentrations are 0, 1/4, 1/16, 1/64, 1/256 and 1/1024 respectively (A-type foot-and-mouth disease virus antibody standard dilution by multiple times). And respectively adding the calibration solutions with different concentrations into sample instillation holes of the assembled detection card, carrying out chromatography for 15 minutes, detecting by using a chromatography scanner, processing the detection results obtained 6 times by using a receiving end, calculating the fluorescence signal intensity values of a detection line and a quality control line corresponding to the standard substance by using the receiving end, carrying out linear regression according to the data to make a standard curve of the A-type foot-and-mouth disease virus antibody, and transmitting the standard curve to a cloud platform for storage by using a client.
The standard curve calculated by the receiving end forms a file and correspondingly generates a bar code, and the receiving end can extract the standard curve from the cloud platform by scanning the bar code.
The use method of the detection card comprises the following steps: a sample to be detected (taking serum as an example) and a sample diluent are diluted according to a ratio of 1:50, 80ul of the diluted sample is added into the sample dripping hole 11, and the sample moves from the sample pad 2 to the absorbent paper 5 under the action of the absorbent paper 5. Carrying out chromatography for 15min under the condition of avoiding strong light irradiation, then detecting the detection card by using a chromatography scanner, obtaining fluorescent signals of a detection line 6 and a quality control line 7 by using the chromatography scanner, transmitting detection data to a receiving end by using the chromatography scanner through Bluetooth, calculating the fluorescent signal intensity value of the detection line and the fluorescent signal intensity value of the quality control line by using a client according to the detection data, obtaining a standard curve of the A type foot and mouth disease virus antibody to be detected from a cloud platform by using the receiving end by scanning bar codes of the batch of detection cards, and calculating the titer of the A type foot and mouth disease virus antibody in the liquid to be detected by using the receiving end according to the contrast relation between the standard curve and the fluorescent signal intensity values of the quality control line and the detection line. The receiving end can be a mobile phone or a tablet computer.
The preparation method of the test paper card comprises the following steps:
firstly, sticking a nitrocellulose membrane on a PVC (polyvinyl chloride) bottom plate, spraying lgG of goat anti-chicken lgY diluted to 0.25mg/ml and A-type foot-and-mouth disease virus epitope recombinant antigen diluted to 0.5mg/ml on the nitrocellulose membrane by adopting a special machine for spotting and gold spraying to form a quality control line and a detection line, wherein the spraying amount is 1 mu l/cm, and then drying at the temperature of 37 ℃ for 8 hours;
step two, preparing lanthanide fluorescent microspheres marked with chicken lgY and lanthanide fluorescent microspheres marked with A-type foot-and-mouth disease virus VP1 recombinant antigen, adding 1mL of lanthanide fluorescent microspheres into 50mg of MES (2- (N-morpholine) ethanesulfonic acid) buffer solution (0.1M, pH7.0), adding 10mg of carbodiimide (EDC) and 10mg of N-hydroxysuccinimide sulfonic acid sodium salt, stirring and dissolving, reacting at room temperature for 30 minutes, then carrying out centrifugal operation, redissolving the centrifugal precipitate with 50mM boric acid buffer solution (pH8.2), adding 2mg of dialyzed chicken lgY, stirring and reacting at room temperature for 24 hours, then centrifuging, sealing, and then preserving in a dilution solution (the preservation environment temperature is 2-8 ℃), thus obtaining the lanthanide fluorescent microspheres marked with chicken lgY; marking lanthanide series fluorescent microspheres of the A type foot-and-mouth disease virus VP1 recombinant antigen by the same method;
thirdly, respectively diluting lanthanide fluorescent microspheres marked with chicken lgY and A-type foot-and-mouth disease virus VP1 recombinant antigen to the concentrations of 0.1 mu g/ml and 3 mu g/ml, spraying the lanthanide fluorescent microspheres on a carrier base layer by adopting a spot-film gold spraying machine to form a marking pad, wherein the spraying amount is 2.5 mu l/cm, and then drying at the temperature of 37 ℃ for 8 hours;
and step four, sequentially adhering the sample pad, the marking pad and the absorbent paper on a PVC base plate to assemble a large card, cutting the large card into a test paper card with the width of 5mm by using a shearing machine, and assembling the test paper card in a shell to obtain the A-type foot-and-mouth disease virus antibody detection card.
The rapid quantitative detection system for the foot-and-mouth disease virus antibody is based on an early-stage field rapid detection and remote online monitoring, early warning, prevention and control system platform, an internet card is accessed into an immunofluorescence instrument, real-time data detection is achieved, real-time uploading monitoring is achieved, risks of introduction of serious animal epidemic viruses such as the foot-and-mouth disease virus 3ABC antibody can be monitored and early warned in a real-time and remote mode, and data support and technical support are provided for purification, prevention and control and monitoring of animal epidemic diseases in China.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (4)
1. A chromatography detection card is characterized by comprising a shell, a test paper card and a supporting device for supporting the test paper card, wherein the supporting device is detachably arranged in the shell, the test paper card comprises a bottom plate with a pressure-sensitive adhesive, a sample pad, a marker pad, a nitrocellulose membrane and absorbent paper are sequentially adhered to the bottom plate, the marker pad comprises a carrier base layer and a marker, the nitrocellulose membrane is coated with a foot-and-mouth disease virus epitope recombinant antigen as a detection line, the nitrocellulose membrane is coated with a rabbit anti-chicken lgY antibody as a quality control line, the marker is a mixture of fluorescent detection microspheres marked with the foot-and-mouth disease virus VP1 recombinant antigen and fluorescent quality control microspheres marked with the chicken lgY antibody, a sample dripping hole is formed in the upper surface of the shell and corresponds to the sample pad of the test paper card on the supporting device, an observation hole is formed in the upper surface of the shell and corresponds to the detection line and the quality control line on the nitrocellulose membrane of the test paper card on the supporting device, the side of the shell is provided with an insertion hole for the supporting device to enter and exit the shell.
2. The chromatography detection card according to claim 1, wherein the supporting device comprises a flat plate, a supporting plate and an adjusting bolt, the supporting plate is used for supporting the test paper card, the supporting plate is arranged above the flat plate, one end of the flat plate is hinged with one end of the supporting plate through a connecting plate, the other end of the flat plate is provided with a through hole, a stud of the adjusting bolt penetrates through the through hole from bottom to top and then interferes with the supporting plate, so that when the stud of the adjusting bolt is screwed upwards, the stud jacks up the supporting plate, and the other end of the supporting plate is inclined upwards relative to the flat plate.
3. The chromatography detection card of claim 2, wherein the plate of the supporting device is provided with a locking hole, the housing is internally provided with a locking spring and a push rod, the locking spring is arranged at one end of the push rod and is used for pushing the other end of the push rod to extend into the locking hole of the plate so as to lock the plate of the supporting device at the working position in the housing.
4. The chromatography detection card according to claim 3, wherein an electromagnet for attracting the lock spring or the lift pin and a return spring for causing the holding means to eject outwardly from the insertion hole are further provided in the housing, and when the holding means is ejected, the electromagnet is energized to generate a magnetic force for attracting the lock spring or the lift pin to cause the lift pin to be pulled out from the lock hole, and the holding means is ejected from the housing from the operating position by an ejecting force applied from the return spring.
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| CN201921266361.5U CN210487788U (en) | 2019-08-07 | 2019-08-07 | Chromatography detection card |
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| CN201921266361.5U CN210487788U (en) | 2019-08-07 | 2019-08-07 | Chromatography detection card |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112379086A (en) * | 2020-11-11 | 2021-02-19 | 中国农业科学院烟草研究所 | A test paper box for detecting remaining acetamiprid |
| CN112881696A (en) * | 2021-01-13 | 2021-06-01 | 北京中检葆泰生物技术有限公司 | Detection method and system for stably detecting aflatoxin content |
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2019
- 2019-08-07 CN CN201921266361.5U patent/CN210487788U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112379086A (en) * | 2020-11-11 | 2021-02-19 | 中国农业科学院烟草研究所 | A test paper box for detecting remaining acetamiprid |
| CN112379086B (en) * | 2020-11-11 | 2024-04-23 | 中国农业科学院烟草研究所 | Test paper box for detecting acetamiprid residues |
| CN112881696A (en) * | 2021-01-13 | 2021-06-01 | 北京中检葆泰生物技术有限公司 | Detection method and system for stably detecting aflatoxin content |
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