CN210458222U - Trace amount DNA extraction capsule - Google Patents

Trace amount DNA extraction capsule Download PDF

Info

Publication number
CN210458222U
CN210458222U CN201921366688.XU CN201921366688U CN210458222U CN 210458222 U CN210458222 U CN 210458222U CN 201921366688 U CN201921366688 U CN 201921366688U CN 210458222 U CN210458222 U CN 210458222U
Authority
CN
China
Prior art keywords
inner tube
push rod
cavity
wall
dna extraction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201921366688.XU
Other languages
Chinese (zh)
Inventor
余家昌
徐泉
张佳
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aoran Technology Biology (shenzhen) Co Ltd
Original Assignee
Aoran Technology Biology (shenzhen) Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aoran Technology Biology (shenzhen) Co Ltd filed Critical Aoran Technology Biology (shenzhen) Co Ltd
Priority to CN201921366688.XU priority Critical patent/CN210458222U/en
Application granted granted Critical
Publication of CN210458222U publication Critical patent/CN210458222U/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Landscapes

  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The utility model relates to a trace DNA extraction capsule, which comprises an inner tube, an outer tube and a top cover, wherein the inner tube is arranged on the upper part of the outer tube in a piston-type sliding manner, the top cover can be detachably covered on the top of the inner tube, the middle part of the top cover is provided with a containing cavity, a reaction reagent cavity is arranged in the containing cavity, the upper part of the containing cavity is provided with a T-shaped push rod in a sliding manner, a separable push rod supporting sleeve is arranged between the push rod and the top of the top cover, a solid reaction reagent is arranged in the reaction reagent cavity, the top of the reaction reagent cavity is provided with a through; and the top cover is provided with a heat sealing film, and the heat sealing film is arranged below the tip end. The utility model discloses an extract the capsule and place solid reactant in the reactant intracavity, then seal up the reactant chamber through the heat-seal membrane and deposit inside the top cap, liquid draws reagent and places in the inner tube, seals through the top cap, therefore solid reactant with draw reagent all can not be polluted before using, improve the inspection accuracy.

Description

Trace amount DNA extraction capsule
Technical Field
The utility model relates to a DNA draws technical field, concretely relates to trace DNA draws capsule.
Background
With the progress of DNA inspection technology, DNA extraction has become a key link influencing the success of inspection. How to extract enough high-quality DNA from trace amount of test material is a major problem in the field of DNA inspection in forensic science.
Patent CN201420415812.8 discloses a single-tube centrifugal casing and extraction is performed, the extraction method is: firstly, the cotton swab wiper/adhesive/adsorbate mixed with the exfoliated cells is put into a filter tube with a filter membrane, the filter tube is directly inserted into a tube cavity of a collecting tube, and then cell lysis solution is added into the filter tube and is placed in a temperature environment of 60-99 ℃ for lysis. And then, centrifuging, collecting cell lysate into a collection tube after centrifugation, and extracting DNA. However, such an extraction device may cause contamination during transportation and use, resulting in errors in the test results.
SUMMERY OF THE UTILITY MODEL
The purpose of the utility model is to provide an extraction capsule which can improve the accuracy of trace DNA test results in order to overcome the defects of the prior art.
In order to achieve the purpose of the present invention, the present application provides the following technical solutions.
In a first aspect, the application provides a trace DNA extraction capsule, which comprises an inner tube, an outer tube and a top cover, wherein the inner tube is arranged on the upper part of the outer tube in a piston-type sliding manner, and the top cover is detachably covered on the top of the inner tube; and the top cover is provided with a heat sealing film, and the heat sealing film is arranged below the tip end.
In an embodiment of the first aspect, the accommodating cavity comprises an upper cavity and a lower cavity which are coaxially arranged, the diameter of the lower cavity is larger than that of the upper cavity, the inner wall of the lower cavity is matched with the outer wall of the reaction reagent cavity, the inner wall of the upper cavity is matched with the outer wall of the push rod, the lower end of the push rod is provided with a push rod sealing ring, and the push rod sealing ring is arranged between the push rod and the upper cavity in an interference manner in an initial state.
In one embodiment of the first aspect, the push rod is provided with an air vent groove in a side wall of an upper middle portion.
In one embodiment of the first aspect, the push rod support sleeve is an annular sleeve with an open side wall.
In an embodiment of the first aspect, the push rod supporting sleeve is provided with a connecting band, one end of the connecting band is fixed on the outer wall of the top cover, and the other end of the connecting band is fixed with the outer wall of the push rod supporting sleeve.
In an embodiment of the first aspect, the top of the outer tube and the upper portion of the inner tube are provided with support tables opposite to each other, the outer wall of the upper portion of the inner tube is provided with a separable inner tube support sleeve, and the top end and the bottom end of the inner tube support sleeve are respectively abutted against the two support tables.
In an implementation manner of the first aspect, an inner tube sealing head is arranged at the bottom of the inner tube, the inner tube sealing head is in a reverse taper shape, an inner tube liquid outlet is arranged at the bottom of the inner tube sealing head, a filter membrane is arranged at the upper end of the inner tube liquid outlet, the filter membrane is pressed at the upper end of the inner tube liquid outlet through an annular pressing ring, and an inner tube sealing ring is arranged between the pressing ring and the filter membrane.
In one embodiment of the first aspect, the pore size of the filter membrane is 0.2 to 0.5 μm.
In an implementation manner of the first aspect, a liquid storage cavity is arranged at the bottom inside the outer tube, the top of the liquid storage cavity is communicated with the liquid outlet of the inner tube, an outer tube liquid outlet is arranged at the bottom of the liquid storage cavity, and the bottom of the outer tube liquid outlet is encapsulated by an outer tube heat sealing film.
In an embodiment of the first aspect, a sheath is sleeved outside the outer tube liquid outlet, a plurality of O-ring seals are disposed between the sheath and the outer wall of the outer tube liquid outlet, and the outer tube heat-sealing film abuts against the bottom of the sheath.
Compared with the prior art, the beneficial effects of the utility model reside in that:
(1) the solid reaction reagent is placed in the reaction reagent cavity, the reaction reagent cavity is sealed inside the top cover through the heat sealing film, the liquid extraction reagent is placed in the inner tube, and the inner tube is sealed through the top cover, so that the solid reaction reagent and the extraction reagent cannot be polluted before use, and the inspection accuracy is improved;
(2) in the storage and transportation stage, the inner pipe, the outer pipe and the top cover are fixed through the push rod supporting sleeve and the inner pipe supporting sleeve, so that the damage is avoided;
(3) the operation is simple and convenient, and other complex tools are not needed.
Drawings
Fig. 1 is a schematic view of the three-dimensional structure of the extraction capsule of the present invention in an unused state;
FIG. 2 is a schematic cross-sectional view of the extraction capsule of the present invention in an unused state;
FIG. 3 is an enlarged schematic view of the reaction reagent chamber of FIG. 2;
fig. 4 is a schematic view of the three-dimensional structure of the extraction capsule of the present invention in the use state;
fig. 5 is a schematic view of the sectional structure of the extraction capsule of the present invention in use.
In the drawing, 1 is a top cover, 11 is a push rod, 111 is an exhaust groove, 12 is a push rod support sleeve, 13 is a connecting belt, 14 is a reaction reagent cavity, 141 is a through hole, 142 is a tip, 143 is a solid reaction reagent, 15 is a heat-sealing film, 16 is a lower cavity, 17 is an upper cavity, 18 is a push rod sealing ring, 19 is a top cover sealing ring, 2 is an inner tube, 21 is an inner tube support sleeve, 22 is an inner tube sealing head, 23 is a filter film, 24 is a pressing ring, 25 is an inner tube sealing ring, 26 is an inner tube liquid outlet, 27 is a support table, 3 is an outer tube, 31 is a liquid storage cavity, 32 is an outer tube liquid outlet, 33 is an outer tube heat-sealing film, 34 is a support table, 4 is a sheath.
Detailed Description
Unless otherwise defined, technical or scientific terms used herein shall have the ordinary meaning as is understood by those of ordinary skill in the art to which the invention belongs. All numerical values recited herein as between the lowest value and the highest value are intended to mean all values between the lowest value and the highest value in increments of one unit when there is more than two units difference between the lowest value and the highest value.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the invention, unless otherwise specified, "a plurality" means two or more.
In the description of the present invention, it should be noted that unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the creation of the present invention can be understood by those of ordinary skill in the art through specific situations.
The application aims to provide a trace DNA extraction capsule, which comprises an inner tube, an outer tube and a top cover, wherein the inner tube is arranged on the upper part of the outer tube in a piston-type sliding manner, the top cover is detachably covered at the top of the inner tube, the extraction capsule is characterized in that a containing cavity is arranged in the middle of the top cover, a reaction reagent cavity is arranged in the containing cavity, a T-shaped push rod is arranged on the upper part of the containing cavity in a sliding manner, a separable push rod supporting sleeve is arranged between the push rod and the top of the top cover, a solid reaction reagent is arranged in the reaction reagent cavity, a through hole is formed in the top of the reaction reagent cavity, and the bottom of the reaction; and the top cover is provided with a heat sealing film, the heat sealing film is arranged below the tip, and meanwhile, a liquid extraction reagent is prestored in the inner tube. In a specific embodiment, the bottom of the top cover is provided with an internal thread, and the heat sealing film is arranged on the upper edge of the internal thread. In an unused state, due to the existence of the push rod supporting sleeve, the push rod cannot move downwards, namely the push rod cannot apply pressure to the reaction reagent cavity, and therefore the reaction reagent cavity is stored in the accommodating cavity under the action of the heat sealing film. Preferably, the solid reaction reagent placed in the reaction reagent cavity is granular, and the particle size of the solid reaction reagent is larger than the diameter of the through hole, so that the solid reaction reagent cannot fall out of the reaction reagent cavity. On the other hand, because the top cap is sealed with the inner tube top, the extraction reagent that exists in the inner tube in advance can not contact with the environment, and the setting of heat-seal membrane for liquid extraction reagent can not get into the top cap inside yet, can not contact with solid reactant promptly. When the extraction capsule is used, the push rod supporting sleeve is removed, then the push rod is pushed downwards, the push rod gives pressure to the reaction reagent cavity, the tip at the bottom of the reaction reagent cavity punctures the heat sealing membrane, and then the whole reaction reagent cavity falls into the inner tube. In the storage process, the reaction reagent cavity is sealed inside the top cover through the heat sealing film, so that the solid reaction reagent cannot be polluted before use, and the inspection accuracy is improved.
In an embodiment of the first aspect, the accommodating cavity comprises an upper cavity and a lower cavity which are coaxially arranged, the diameter of the lower cavity is larger than that of the upper cavity, the inner wall of the lower cavity is matched with the outer wall of the reaction reagent cavity, the inner wall of the upper cavity is matched with the outer wall of the push rod, the lower end of the push rod is provided with a push rod sealing ring, and the push rod sealing ring is arranged between the push rod and the upper cavity in an interference manner in an initial state. Under the unused state, because the existence of push rod sealing washer, cavity of resorption is isolated with external, therefore the solid reactant in the reactant intracavity can not receive the pollution.
In one embodiment of the first aspect, the push rod is provided with an air vent groove in a side wall of an upper middle portion. When extracting the capsule and using, the push rod moves down, until the reaction reagent chamber falls into the inner tube, at this moment, the push rod sealing washer has been in the intracavity down, can't play sealed effect. Meanwhile, due to the existence of the exhaust groove, a gap is reserved between the push rod and the inner wall of the upper cavity, and therefore the inner tube is communicated with the atmosphere. This is because the solid reaction reagent usually needs to be heated and cracked to extract the trace amount of DNA, and then centrifugal separation is performed, a certain amount of gas is generated during the heating process, if the inner tube is in a closed state, the inner tube has a large air pressure, which is not only easy to burst, but also more important, the inner tube may break through the filter membrane, and the solid-liquid separation cannot be performed, thereby affecting the extraction of the trace amount of DNA. The device enables the inner pipe to be communicated with the atmosphere through the exhaust groove, so that the condition can be effectively avoided.
In one embodiment of the first aspect, the push rod support sleeve is an annular sleeve with an open side wall. This arrangement makes it more convenient for the push rod support sleeve to be removed.
In an embodiment of the first aspect, the push rod supporting sleeve is provided with a connecting band, one end of the connecting band is fixed on the outer wall of the top cover, and the other end of the connecting band is fixed with the outer wall of the push rod supporting sleeve.
In an embodiment of the first aspect, the top of the outer tube and the upper portion of the inner tube are provided with support tables opposite to each other, the outer wall of the upper portion of the inner tube is provided with a separable inner tube support sleeve, and the top end and the bottom end of the inner tube support sleeve are respectively abutted against the two support tables. This setting can guarantee the utility model discloses an extract capsule inner tube can not the downstream when not using, guarantees that the outer tube bottom has the stock solution chamber in enough space.
In an implementation manner of the first aspect, an inner tube sealing head is arranged at the bottom of the inner tube, the inner tube sealing head is in a reverse taper shape, an inner tube liquid outlet is arranged at the bottom of the inner tube sealing head, a filter membrane is arranged at the upper end of the inner tube liquid outlet, the filter membrane is pressed at the upper end of the inner tube liquid outlet through an annular pressing ring, and an inner tube sealing ring is arranged between the pressing ring and the filter membrane.
In one embodiment of the first aspect, the pore size of the filter membrane is 0.2 to 0.5 μm. Under the centrifugal action, the liquid with trace DNA is extracted and enters the liquid storage cavity through the filter membrane, and the solid stays in the inner tube, so that the solid-liquid separation is realized.
In an implementation manner of the first aspect, a liquid storage cavity is arranged at the bottom inside the outer tube, the top of the liquid storage cavity is communicated with the liquid outlet of the inner tube, an outer tube liquid outlet is arranged at the bottom of the liquid storage cavity, and the bottom of the outer tube liquid outlet is encapsulated by an outer tube heat sealing film. The outer tube heat-sealing film is arranged to avoid the influence on the inspection accuracy caused by the fact that pollutants enter the liquid storage cavity when the outer tube heat-sealing film is not used. After the liquid dissolved with DNA enters the liquid storage cavity, the subsequent inspection device punctures the outer tube heat-sealing film, so that the liquid directly enters the inspection device, and no additional pollution is caused.
In an embodiment of the first aspect, a sheath is sleeved outside the outer tube liquid outlet, a plurality of O-ring seals are disposed between the sheath and the outer wall of the outer tube liquid outlet, and the outer tube heat-sealing film abuts against the bottom of the sheath. The sheath is provided to protect the outer tube heat seal film from being damaged.
Examples
The embodiments of the present invention will be described in detail with reference to the accompanying drawings, which are implemented on the premise of the technical solution of the present invention, and the detailed embodiments and the specific operation processes are given, but the scope of the present invention is not limited to the following embodiments.
Example 1
The structure of the trace DNA extraction capsule is shown in figures 1, 2, 4 and 5, and comprises an inner tube 2, an outer tube 3 and a top cover 1, wherein the inner tube 2 is arranged on the upper part of the outer tube 3 in a piston-type sliding manner, the top cover 1 is detachably covered on the top of the inner tube 2, the inner wall of the bottom of the top cover 1 is provided with an internal thread, the upper edge of the internal thread is provided with a heat sealing film 15, a top cover sealing ring 19 is arranged between the heat sealing film 15 and the top cover 1, the middle part of the top cover 1 is provided with a containing cavity, a reaction reagent cavity 14 is arranged in the containing cavity, the upper part of the containing cavity is provided with a T-shaped push rod 11 in a sliding manner, a detachable push rod supporting sleeve 12 is arranged between the push rod 11 and the top of the top cover 1, a solid reaction reagent 143 is arranged in the reaction reagent cavity 14, the top of the.
In this embodiment, in order to avoid the solid reactive agent 143 from sending leaks during capsule transport, the particle size of the solid reactive agent 143 is larger than the maximum size of the through-hole 141. Further, in this embodiment, in order to ensure that the solid reaction reagent 143 in the reaction reagent chamber 14 can be sufficiently dissolved in the liquid extraction reagent, the reaction reagent chamber 14 is made of a material having a density greater than that of the liquid extraction reagent. So that it will sink to the bottom of the inner tube rather than float above the liquid extraction reagent level as the reagent chamber 14 drops into the inner tube.
The accommodating cavity comprises an upper cavity 17 and a lower cavity 16 which are coaxially arranged, the diameter of the lower cavity 16 is larger than that of the upper cavity 17, the inner wall of the lower cavity 16 is matched with the outer wall of the reaction reagent cavity 14, the inner wall of the upper cavity 17 is matched with the outer wall of the push rod 11, a push rod sealing ring 18 is arranged at the lower end of the push rod 11, and in an initial state, the push rod sealing ring 18 is arranged between the push rod 11 and the upper cavity 17 in an interference mode. The side wall of the middle upper part of the push rod 11 is provided with an exhaust groove.
The push rod support sleeve 12 is an annular sleeve with an opening on the side wall. The push rod supporting sleeve 12 is provided with a connecting belt 13, one end of the connecting belt 13 is fixed on the outer wall of the top cover 1, and the other end of the connecting belt 13 is fixed with the outer wall of the push rod supporting sleeve 12.
The top of outer tube 3 and the upper portion of inner tube 2 set up the brace table relatively, and the outer wall on inner tube 2 upper portion sets up separable inner tube support cover 21, and the top and the bottom of inner tube support cover 21 respectively with two brace tables butt.
The bottom of inner tube 2 is equipped with inner tube sealing head 22, and inner tube sealing head 22 is the back taper, and the bottom of inner tube sealing head 22 is equipped with inner tube liquid outlet 26, and the upper end of inner tube liquid outlet 26 is equipped with filter membrane 23, and filter membrane 23 is pressed in inner tube liquid outlet 26 upper end through annular clamping ring 24, and is equipped with inner tube sealing washer 25 between clamping ring 24 and the filter membrane 23. The aperture of the filter membrane 23 is 0.2-0.5 μm.
The bottom in the outer tube 3 is equipped with liquid storage cavity 31, and the top and the inner tube liquid outlet 26 intercommunication in liquid storage cavity 31, and the bottom in liquid storage cavity 31 is equipped with outer tube liquid outlet 32, and outer tube heat-seal membrane 33 encapsulation is passed through to the bottom of outer tube liquid outlet 32.
The outer sleeve of outer tube liquid outlet 32 is equipped with sheath 4, is equipped with a plurality of O type sealing washers 41 between sheath 4 and the outer wall of outer tube liquid outlet 32, and outer tube heat-seal membrane 33 is with the bottom butt of sheath 4.
The working principle of the extraction capsule is as follows:
when the extraction capsule is not in use, the solid reagent 143 in the form of granules is placed in the reagent chamber 14, and the reagent chamber 14 is placed in the lower chamber 16 and supported by the heat-sealing film 15. The liquid extraction reagent is pre-stored in the inner tube 2, and the top cap 1 is screwed on the top of the inner tube 2. The push rod support sleeve 12 is sleeved outside the push rod 11, so that the push rod 11 cannot move downwards. An inner tube support sleeve 21 is provided between the support platform 27 of the inner tube 2 and the support platform 34 of the outer tube 3 such that the inner tube 2 cannot move downward, as shown in fig. 1 and 2.
When the trace DNA is required to be extracted, the top cover 1 is rotated to be separated from the inner tube 2, an object with the trace DNA is placed in the inner tube 2, and then the top cover 1 is covered on the top of the inner tube 2. Then the push rod 11 is pushed downwards, the push rod 11 presses the reaction reagent chamber 14 downwards, the tip 142 at the bottom of the reaction reagent chamber 14 pierces the heat seal film 15, and finally falls into the inner tube 2. The extraction reagent enters the reaction reagent chamber 14 from the through hole 141 at the top of the reaction reagent chamber 14, and dissolves the solid reaction reagent 143 therein. The extraction capsule is cracked at the temperature of 60-99 ℃, then the extraction capsule is centrifuged, in the process, the solid reaction reagent 143 is uniformly dissolved in the solvent, and the trace amount of DNA in the object is extracted. At the same time, the solution penetrates the filter membrane 23 and enters the reservoir chamber 31 due to the centrifugal force. After the centrifugation is finished, the sheath 4 is removed, a subsequent inspection device punctures the outer tube heat sealing film 33 and is communicated with the liquid storage cavity 31, then the inner tube supporting sleeve 21 is removed, the top cover 1 is pushed downwards, the inner tube 2 moves downwards along with the inner tube, the volume of the liquid storage cavity 31 gradually changes downwards until the supporting platform 27 of the inner tube 2 is contacted with the supporting platform 34 of the outer tube 3, the inner tube sealing head 22 is indicated to be contacted with the bottom of the outer tube 3, the volume of the liquid storage cavity 31 is almost 0, all the solution enters the subsequent inspection device, and the capsule extraction process is finished as shown in fig. 4 and 5.
The embodiments described above are intended to facilitate the understanding and appreciation of the application by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present application is not limited to the embodiments herein, and those skilled in the art who have the benefit of this disclosure will appreciate that many modifications and variations are possible within the scope of the present application without departing from the scope and spirit of the present application.

Claims (10)

1. A trace DNA extraction capsule comprises an inner tube, an outer tube and a top cover, wherein the inner tube is arranged on the upper portion of the outer tube in a piston-type sliding mode, and the top cover is detachably covered on the top of the inner tube; and the top cover is provided with a heat sealing film, and the heat sealing film is arranged below the tip end.
2. The trace DNA extraction capsule according to claim 1, wherein the containing cavity comprises an upper cavity and a lower cavity which are coaxially arranged, the diameter of the lower cavity is larger than that of the upper cavity, the inner wall of the lower cavity is matched with the outer wall of the reaction reagent cavity, the inner wall of the upper cavity is matched with the outer wall of the push rod, a push rod sealing ring is arranged at the lower end of the push rod, and the push rod sealing ring is arranged between the push rod and the upper cavity in an interference manner in an initial state.
3. The trace DNA extraction capsule according to claim 2, wherein the push rod has a vent groove in a side wall of an upper middle portion thereof.
4. The trace DNA extraction capsule according to claim 1, wherein the push rod support sleeve is an annular sleeve with an open side wall.
5. The trace DNA extraction capsule according to claim 1 or 4, wherein the push rod support sleeve is provided with a connecting band, one end of the connecting band is fixed on the outer wall of the top cover, and the other end of the connecting band is fixed with the outer wall of the push rod support sleeve.
6. The trace DNA extraction capsule according to claim 1, wherein support platforms are disposed opposite to the top of the outer tube and the upper portion of the inner tube, a separable inner tube support sleeve is disposed on the outer wall of the upper portion of the inner tube, and the top end and the bottom end of the inner tube support sleeve are respectively abutted against the two support platforms.
7. The trace DNA extraction capsule according to claim 1, wherein the inner tube has a bottom portion provided with an inner tube sealing head, the inner tube sealing head has an inverted cone shape, the bottom portion of the inner tube sealing head is provided with an inner tube liquid outlet, the upper end of the inner tube liquid outlet is provided with a filter membrane, the filter membrane is pressed against the upper end of the inner tube liquid outlet by an annular pressing ring, and an inner tube sealing ring is provided between the pressing ring and the filter membrane.
8. The trace DNA extraction capsule according to claim 7, wherein the pore size of the filter membrane is 0.2 to 0.5 μm.
9. The trace DNA extraction capsule according to claim 7, wherein a liquid storage cavity is formed in the bottom of the outer tube, the top of the liquid storage cavity is communicated with the liquid outlet of the inner tube, an outer tube liquid outlet is formed in the bottom of the liquid storage cavity, and the bottom of the outer tube liquid outlet is sealed by an outer tube heat sealing film.
10. The trace DNA extraction capsule according to claim 9, wherein a sheath is sleeved outside the outer tube liquid outlet, a plurality of O-ring seals are disposed between the sheath and the outer wall of the outer tube liquid outlet, and the outer tube heat sealing film abuts against the bottom of the sheath.
CN201921366688.XU 2019-08-22 2019-08-22 Trace amount DNA extraction capsule Active CN210458222U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201921366688.XU CN210458222U (en) 2019-08-22 2019-08-22 Trace amount DNA extraction capsule

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201921366688.XU CN210458222U (en) 2019-08-22 2019-08-22 Trace amount DNA extraction capsule

Publications (1)

Publication Number Publication Date
CN210458222U true CN210458222U (en) 2020-05-05

Family

ID=70452751

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201921366688.XU Active CN210458222U (en) 2019-08-22 2019-08-22 Trace amount DNA extraction capsule

Country Status (1)

Country Link
CN (1) CN210458222U (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110373411A (en) * 2019-08-22 2019-10-25 奥然科技生物(深圳)有限责任公司 A kind of trace amount DNA extraction capsule
CN114870924A (en) * 2022-04-29 2022-08-09 宁波中盛产品检测有限公司 Combined centrifugal tube for QuEChERS extraction method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110373411A (en) * 2019-08-22 2019-10-25 奥然科技生物(深圳)有限责任公司 A kind of trace amount DNA extraction capsule
CN114870924A (en) * 2022-04-29 2022-08-09 宁波中盛产品检测有限公司 Combined centrifugal tube for QuEChERS extraction method
CN114870924B (en) * 2022-04-29 2023-05-26 宁波中盛产品检测有限公司 Combined centrifuge tube for QuEChERS extraction method

Similar Documents

Publication Publication Date Title
CN210458222U (en) Trace amount DNA extraction capsule
CN203894092U (en) Filtering membrane interlayer cup
CN101992136A (en) Combined centrifuge tube for preparing cell wax blocks by using exfoliated cells
CN107308706A (en) Multistage filter device for water quality analysis sample in positive and negative pressure laboratory
CN204073506U (en) A kind of miniature decontaminating column of impurity absorption type of pipettor direct injected
CN106289396A (en) Detection and analysis device for measuring total gas production rate and gas production components in battery
CN110373411A (en) A kind of trace amount DNA extraction capsule
CN109738237B (en) Liquid collecting device
CN105651554A (en) Collection device and collection method of concrete pore solution
WO2023160091A1 (en) Purification column
CN217438172U (en) Cell sample draws subassembly
CN115078348B (en) Accurate and rapid detection device and detection method thereof
CN106769346B (en) Method for analyzing hydrogen isotopes in water
CN206676374U (en) A kind of jacketed reaction bottle
CN212779745U (en) Pipe sealing performance testing machine
CN209241569U (en) Cytological sample collection bottle
CN209791572U (en) Biological sample save set
CN215877983U (en) Saturated absorption reaction vessel
CN216604247U (en) Liquid phase solvent filtering device
CN220104515U (en) Quantitative fecal sampling device
CN220926777U (en) Integrated nucleic acid detection kit
CN213180728U (en) Online sampler
CN216082226U (en) Gas sampling pipe convenient to collect absorption liquid
CN221286952U (en) Extraction device
CN217505396U (en) Multifunctional vacuum quantitative concentrator

Legal Events

Date Code Title Description
GR01 Patent grant
GR01 Patent grant